CN1274429C - Two-stage method of applying white rot fungus to degrade hard-to-degrade environment pollutant - Google Patents

Abstract

The present invention relates to a two-stage method which uses white rot fungi to degrade environmental pollutants that are hard to degrade and belongs to the technical field of processing environmental pollutants. In order to solve the problem that the conditions of applying white rot fungi are very severe, the present invention researches the physiological characteristics and the biochemical characteristics of white rot fungi and raises the two-stage method which adopts an intermittent operation mode to separate the stages of growth and degradation. In the method, energy consumption can be reduced by ways of high temperature cultivation and normal temperature degradation; cost is reduced by using waste corn cobs and lumber as the nutrient source; high nutrient concentration promotes growth, and low nutrient concentration promotes degradation; energy consumption can be reduced in growth phase by way of natural ventilation; the addition of carriers can maintain high concentration exocellular enzyme, quicken the reaction rate, maintain high decolorizing efficiency for a long period and solve the problem of muddy water separation; the mixing use of non-inert carriers and inert carriers is beneficial for supplying oxygen and maintaining good hydraulic characteristics. In addition, the present invention has strong capability of resisting the pollution of sundry bacteria.

Description

Use the dual stage process of white rot fungus degrading difficult degradation environmental contaminants

Technical field

The invention belongs to the environmental contaminants processing technology field.

Background technology

White-rot fungi Phanerochaete chrysosporium is subjected to extensive concern because of it has to the very strong degradation capability of hardly degraded organic substance.Big quantity research adopts various reactors that different target compounds is degraded, and has obtained degradation effect preferably mostly, but the comparatively harsh problem of ubiquity reaction condition is the major obstacle of large-scale practical application white rot fungus degrading environmental contaminants.

Existing white rot fungi reactor adopts growth and degradation process unified method more: higher nutrient concentrations can promote the growth of white-rot fungi, but can suppress the startup of secondary metabolism; The optimum growth temp of white-rot fungi is 35 ℃-39 ℃, and most reactors adopt this temperature conditions, and whole process keeps higher temperature, waste water is heated up will bring huge energy consumption; All the time need the aeration intensity that keeps higher, the subject matter that need solve when also being practical application in the reactor.

Summary of the invention

In order to solve the too harsh problem of white-rot fungi application conditions, the present invention studies the physio-biochemical characteristics of white-rot fungi, has compared the influence of environmental factor to white rot conk and decolorization, has proposed the operation process of suggestion.See table 1 for details.

Table 1 environmental factor is to the operation process of the influence and the suggestion of white rot conk and decolorization

Influence factor	Growth	Decolouring	Technology
				The natural lixivium oxygen-supplying amounts such as temperature carbon source nitrogenous source phosphorus source timber corncob add carrier	+ + + + + 0 +	0 - - 0 0 + +	High growth temperature, normal temperature decolours and grows under the higher carbon source condition, grow under the higher nitrogenous source condition of decolouring under the low carbon source condition, grow under the condition of decolouring higher phosphorous source under the low nitrogenous source condition, timber is added in decolouring under the condition of more low-phosphorous source, corncob dry or leachate incubation need not stir, and degradation process adopts to stir adds the carrier cultivation

Annotate :+-positive correlation--negative correlation 0-is irrelevant

The factor that pair influences white-rot fungi growth and degradation process by table 1 compares, and factors such as discovery temperature, nutrient, natural leachate, stirring and interpolation carrier have bigger difference to the influence degree of white rot conk and decoloring ability.This different problem that influences, in treatment process, can solve by mode stage by stage, adopt two-stage process, to grow and decolorization separate, the various influence factors of artificial regulatory make growth and decoloring ability all reach optimized purpose, avoid overall process to adopt very harsh condition to bring too high operating cost simultaneously.

According to above-mentioned research, the present invention proposes following technical scheme.

Use the dual stage process of white rot fungus degrading difficult degradation environmental contaminants, it is characterized in that: described dual stage process adopts intermittent operation mode, will grow and degradation period is separately carried out, and may further comprise the steps in regular turn:

1) the white-rot fungi spore is put into the deionized water vibration and shaken up, make spore liquid;

2) select for use timber and corncob as nutrient source and non-inert carrier, it is mixed with inert carrier commonly used, be immersed in then and finish inoculation in the spore liquid;

3) will inoculate good carrier and place reactor, the gravity-flow ventilation oxygen supply is cultivated under 35～39 ℃ condition, finishes biofilm and starts;

4) in degradation period, the waste water that will contain the difficult degradation environmental contaminants feeds in the reactor, the operation of normal temperature aeration, treat degraded finish after draining;

5) in growth phase, the reactor that drains water is put the gravity-flow ventilation oxygen supply, cultivate under 35～39 ℃ the condition, finish growth phase;

6) degradation period and the 5th of step continuation repetition alternate run the 4th)) growth phase of step is realized effective degraded of difficult degradation environmental contaminants.

The present invention has following significant technique effect:

1) normal temperature operation can be avoided high energy consumption problem that waste water containing dye is heated up and brings.

2) utilize agricultural wastes corncob and timber can reduce the feed supplement cost for nutrient source;

3) cultivate the promotion growth under the condition of high-nutrient concentration, degraded promotes degraded under the condition of low concentration nutrient concentrations;

4) system has stronger anti-living contaminants ability;

5) growth phase gravity-flow ventilation cuts down the consumption of energy significantly.

6) add the ectoenzyme that carrier can be kept higher concentration, accelerate reaction rate; Keep higher decolorizing efficiency over a long time; Solve the mud-water separation problem.

7) non-inert carrier is mixed mutually with inert carrier, and the blockage problem that can avoid corncob to subside and cause helps oxygen supply and keeps hydraulic characteristic preferably.

The specific embodiment

Below in conjunction with concrete example the present invention is described.

Running example: the dye activity bright red with difficult degradation is a target compound, adopts two-stage process to use white rot fungus degrading difficult degradation pollutant.The hardly degraded organic substance kind is a lot, the difficult degradation pollutant that dyestuff belongs to is more common, discharge capacity is big, harm is bigger, reactive dye belong to one of kind that use amount and turnover rate are the highest in the dyestuff, reactive brilliant red belongs to more common type in reactive dye, so the most representative reactive brilliant red of this example employing is a target compound.

Filler: add polyurethane foam, wood particle and corncob fritter, be cut into 1 * 1 * 1 centimeter square size, mixed in equal amounts is even, is filled into diameter and is in 12 centimetres the cylindrical filter post, and packing volume is 0.6L.Polyurethane foam belongs to one of inert carrier commonly used, selects for use dissimilar carriers that the operational effect of reactor is not had special influence.

1) white-rot fungi in the plating medium is scraped with oese, put into the deionized water vibration and shake up, make spore liquid;

2) select for use timber and corncob as nutrient source and non-inert carrier, it is mixed with inert carrier commonly used, be immersed in then and finish inoculation in the spore liquid;

3) will inoculate good carrier and place reactor, the gravity-flow ventilation oxygen supply was cultivated 3 days under 35～39 ℃ condition, finished biofilm and started; The fate that biofilm starts was generally selected for use 3-5 days, and the time is to the not special influence of reactor effect.

4) in degradation period, the waste water that will contain difficult degradation environmental contaminants reactive brilliant red 40mg/L feeds in the reactor, the operation of normal temperature aeration, hydraulic load is 0.42L/Lh, 2 hours time of staying, treat degraded finish after draining; The waste water that will contain difficult degradation environmental contaminants reactive brilliant red 40mg/L once more feeds in the reactor, the operation of normal temperature aeration, hydraulic load is 0.42L/Lh, 2 hours time of staying, treat degraded finish after draining.

5) in growth phase, the reactor that drains water is put the gravity-flow ventilation oxygen supply, cultivated 1 day under 35～39 ℃ the condition, finish growth phase;

6) the 4th) degradation period of step and the 5th) the growth phase alternate run of step is realized effective degraded of difficult degradation environmental contaminants.

This example has moved 7 cycles altogether, and concrete operational factor sees Table 2.

Table 2 two-stage process result of trial operation

Stage	Water inlet reactive brilliant red concentration (mg/L)	Water outlet reactive brilliant red concentration (mg/L)	Clearance (%)	Hydraulic detention time (h)	Hydraulic load (L/Lh)	Running temperature
							The decolouring decolouring is cultivated the decolouring decolouring and is cultivated the decolouring decolouring	40mg/L 40mg/L - 40mg/L 40mg/L - 40mg/L 40mg/L	3.02mg/L 5.23mg/L - 3.1mg/L 4.68mg/L - 4.8mg/L 7.55mg/L	92.3 86.9 92.3 88.3 88 81.1	22 incubation time 24h, 22 incubation time 24h 22	0.42 0.42 - 0.42 0.42 - 0.42 0.42	16℃ 16℃ 35℃ 14℃ 14℃ 35℃ 12.5℃ 12.5℃

Cultivation decolouring decolouring is cultivated to decolour and is cultivated decolouring decolouring cultivation decolouring

- 40mg/L 40mg/L - 40mg/L 40mg/L - 40mg/L 40mg/L - 40mg/L 40mg/L

- 3.52mg/L 6.32mg/L - 3.02mg/L 4.8mg/L - 4.25mg/L 6.53mg/L - 5.6mg/L 8.95mg/L

91.2 84.2 92.5 88 89.4 83.7 86 77.6

Incubation time 24h 22 incubation time 24h 22 incubation time 24h 22 incubation time 24h 22

- 0.42 0.42 - 0.42 0.42 - 0.42 0.42 - 0.42 0.42

35℃ 16℃ 16℃ 35℃ 14℃ 14℃ 35℃ 13.5℃ 13.5℃ 35℃ 13℃ 13℃

Cultivation, twice decolouring are one-period, have carried out 7 cycles altogether.In 14 decolorizations, 2 hours average percent of decolourization can reach 87.25%, and decolorizing efficiency is higher, and bleaching time is shorter.

Test in addition shows, two-phase method is used other difficult degradation environmental contaminants of white rot fungus degrading, such as polycyclic aromatic hydrocarbon, chlorophenol etc. good effect is arranged also.

Claims (1)

1. use the dual stage process of white rot fungus degrading difficult degradation environmental contaminants, it is characterized in that: described dual stage process adopts intermittent operation mode, will grow and degradation period is separately carried out, and may further comprise the steps in regular turn:

1) the white-rot fungi spore is put into the deionized water vibration and shaken up, make spore liquid;

2) select for use timber and corncob as nutrient source and non-inert carrier, it is mixed with inert carrier commonly used, be immersed in then and finish inoculation in the spore liquid;

3) will inoculate good carrier and place reactor, the gravity-flow ventilation oxygen supply is cultivated under 35～39 ℃ condition, finishes biofilm and starts;

4) in degradation period, the waste water that will contain the difficult degradation environmental contaminants feeds in the reactor, the operation of normal temperature aeration, treat degraded finish after draining;

5) in growth phase, the reactor that drains water is put the gravity-flow ventilation oxygen supply, cultivate under 35～39 ℃ the condition, finish growth phase;

6) degradation period and the 5th of step continuation repetition alternate run the 4th)) growth phase of step is realized effective degraded of difficult degradation environmental contaminants.