CN1267561C - Method of bological transforming selenium using bacillus bifidus and its product - Google Patents
Method of bological transforming selenium using bacillus bifidus and its product Download PDFInfo
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- CN1267561C CN1267561C CNB2004100023813A CN200410002381A CN1267561C CN 1267561 C CN1267561 C CN 1267561C CN B2004100023813 A CNB2004100023813 A CN B2004100023813A CN 200410002381 A CN200410002381 A CN 200410002381A CN 1267561 C CN1267561 C CN 1267561C
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Abstract
The present invention discloses a method for biotransformation of selenium by utilizing bacillus bifidus and a product produced by the method. The method for biotransformation of selenium by utilizing bacillus bifidus is characterized in that bacillus bifidus is cultured in a selenium-enriched culture solution to prepare a bacillus bifidus selenium-enriched viable bacterium preparation. The method aims to utilize the bacillus bifidus with physiological character to convert inorganic selenium in the culture solution into low-poisonous thallus cells and organic selenium, and the anticancer function of the thallus cells and the organic selenium are superimposed with the micro ecological regulation function of the bacillus bifidus to produce a selenium-enriched bacillus bifidus viable bacterium preparation with single strain. The product produced by the method has the functions of cancer resistance and cancer prevention, and a selenium carrier of the preparation adopts the bacillus bifidus which is a normal physiological bacterium for human bodies. The product has an important function in the aspect of enhancing the immunity of a body.
Description
Technical field:
The present invention relates to utilize bifidus bacillus that inorganic elements is carried out the method and the product of biological organic transformation, especially relate to a kind of bifidus bacillus carries out bio-transformation to selenium method and products thereof of utilizing.
Background technology:
Selenium is the trace element of needed by human, is a kind of multi-functional life nutritive element, its character between metal and nonmetal between.An amount of selenium have remove interior free yl, anti-oxidant, suppress that lipid peroxide produces, the function of enhancing immunity, protect cytolemma in addition, strengthen that prostate gland is regulated, prevented blood clot, removes cholesterol, promotes cellular uptake sugar, stimulates circulation, prevents skin aging, reduces that luring of carcinogenic substance is carcinous, the cancer cells of removing vivo mutations, the functions such as merisis that prevent cancer cells.Have in the world in more than 40 the national soil to lack selenium, there is 3/4 the scarce selenium area of area genus in China, is northeast---and southwest is moved towards.He result of investigation shows that the crowd of low selenium area life, cancer morbidity is apparently higher than other areas.
For bifidus bacillus, from suckling infant's ight soil, isolate in the nearly century of such bacterium from the Tissier of Pasteur Institut in 1899, to the characteristic of bifidus bacillus, especially carried out a large amount of research work with HUMAN HEALTH relation and application thereof.Since the fifties in last century, because the frequent use of microbiotic and immunosuppressor, impelling scientist is main many quite successful researchs of having done with the intestinal microflora.Wherein bifidus bacillus is as the important microorganism of a class in human intestine's normal microflora, and they comprise that to the beneficial effect of human body anti-tumor function all done abundant affirmation in many works and article.
Summary of the invention:
The object of the present invention is to provide the method for utilizing bifidus bacillus selenium to be carried out bio-transformation.
Another object of the present invention is to provide the product that utilizes bifidus bacillus selenium to be carried out bio-transformation.
First purpose of the present invention is implemented by following technical scheme: utilize bifidus bacillus to the method that selenium carries out bio-transformation, include the seed liquor preparation that contains selenium; Fermentation; Centrifugal collection bacterium; The bacterium mud lyophilize that centrifugal collection bacterium is obtained obtains the viable bacteria powder; Pulverize above gained viable bacteria powder, sieve, above gained viable bacteria powder and auxiliary material mixed preparing and packing;
(1), contain the seed liquor preparation of selenium: bifidobacterium strains is inoculated in the TPY liquid nutrient medium, and wherein the volume ratio of inoculum size is 0.5%-12%, and 37 ± 1 ℃ of anaerobism are cultivated 8-16h, are the one-level kind; Again it is carried out enlarged culturing and become secondary kind, three grades of kinds, the volume ratio of inoculum size is 5%-12%, and wherein the volume ratio of inoculum size promptly: upper level seed liquor and the volume ratio that is access in substratum; The Na of every grade of seed liquor wherein
2SeO
3Content is 5-15 μ g/ml;
Wherein said TPY substratum consisting of by weight:
Lactose 0.2-5.0
Casein peptone 0.2-5.0
Soy peptone 0.01-5.0
Extractum carnis 0.01-5.0
Yeast powder 0.01-5.0
Na
2HPO
4 0.01-1.0
KH
2PO
4 0.01-1.0
MgSO
4 0.005-0.4
L-Cys 0.005-0.2
Tween80 0.001-0.1；
(2) preparation of fermentation liquid: the volume ratio by inoculum size is 5%-12%, and wherein the volume ratio of inoculum size is the volume ratio of three grades of seed liquor and TPY fermented liquid, three grades of seeds is inoculated in contain Na
2SeO
3In the TPY fermented liquid of 5-15g/ml, 37 ± 1 ℃ of anaerobism cultivate 7---15h;
(3), centrifugal collection bacterium: medium centrifugal concentrates, and collects bacterium mud;
(4), bacterium mud lyophilize that centrifugal collection bacterium is obtained obtains the viable bacteria powder, contains selenium and is higher than 50 μ g/g, the bifidobacteria viable bacteria number is higher than 1,000 hundred million/g; Wherein lyophilize: by volume with lyophilized vaccine and bacterium mud:
Bacterium mud: protective material=10-30: 1 (V/V) adds lyophilized vaccine, and thorough mixing is even, carries out vacuum lyophilization, is lower than taking-up in 3% o'clock to bacterium powder moisture content; Pulverize the freeze-dried vaccine powder, cross 40 mesh sieves;
Lyophilized vaccine consisting of by weight wherein:
Vc 0.1-4.0
Monosodium glutamate 0.1-4.0
Skimming milk 5-20
Lactose 5-20;
Most preferably value is Vc: monosodium glutamate: skimming milk: lactose: purified water is 1: 1: 12: 8: 78.
(5), pulverize to live, sieve, medicinal powder preparation and packing.
Described bifidus bacillus can be bifidobacterium adolescentis, bifidumbacterium bifidum, bifidobacteria infantis and bifidus longum bb.
Described TPY substratum composition by weight most preferably is worth:
Lactose 1
Casein peptone 1
Soy peptone 0.5
Extractum carnis 0.5
Yeast powder 0.5
Na
2HPO
4 0.25
KH
2PO
4 0.25
MgSO
4 0.01
L-Cys 0.05
Tween80 0.025。
Contain in the seed liquor preparation of selenium, wherein the Na of every grade of seed liquor
2SeO
3It is 10 μ g/ml that content most preferably is worth.
Utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with capsule preparations, by weight, its component is:
The rich selenium bacterium powder of bifidus bacillus (viable bacteria powder) is more than 2
Magnesium Stearate 0.2-0.5
Lactose 20-40
Starch 40-60
Oligomeric isomaltose 10-30.
Utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with capsule preparations, by weight, its component that most preferably is worth is:
The rich selenium bacterium powder of bifidus bacillus (viable bacteria powder) 5
Magnesium Stearate 0.4
Lactose 30
Starch 50
Oligomeric isomaltose 20.
Utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with tablet formulation, by weight, its component is:
The rich selenium bacterium powder of bifidus bacillus (viable bacteria powder) is more than 5
Lactose 20-30
Glucose 15-25
Magnesium Stearate 0.2-0.6
Microcrystalline Cellulose 5-15
Sodium starch glycolate 4-8
Micropowder silica gel 0.01-0.1.
Utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with tablet formulation, by weight, its component that most preferably is worth is:
The rich selenium bacterium powder of bifidus bacillus (viable bacteria powder) 10
Lactose 25
Glucose 22
Magnesium Stearate 0.48
Microcrystalline Cellulose 10
Sodium starch glycolate 6
Micropowder silica gel 0.07.
Utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with powder or granule, by weight, its component is:
The rich selenium bacterium powder of bifidus bacillus (viable bacteria powder) is more than 2
Lactose 75-95
Micropowder silica gel 0.01-0.1.
Utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with powder or granule, by weight, its component that most preferably is worth is:
The rich selenium bacterium powder of bifidus bacillus (viable bacteria powder) 2
Lactose 90
Micropowder silica gel 0.07.
Advantage of the present invention is: bifidus bacillus is carried out rich selenium cultivate, make the rich selenium active bacteria formulation of bifidus bacillus, be intended to utilize the physiological bifidus bacillus that the inorganic selenium in the nutrient solution is converted into nontoxic somatic cells organoselenium, the two anti-cancer function and the little ecological regulatory function of bifidus bacillus is superimposed, produce rich selenium bifidus bacillus single strain active bacteria formulation, the product of being produced by this method not only has the cancer-resisting function, and the selenium carrier of said preparation is selected bifidus bacillus, this bacterium is a human body normal physiological bacterium, has important function aspect the enhancement immunity of organisms.
Embodiment:
Embodiment 1:
At first with the bifidus longum bb inoculation in the TPY liquid nutrient medium, the volume ratio of inoculum size is that 0.5%, 37 ± 1 ℃ of anaerobism is cultivated 8h, is the one-level kind; Again it is carried out enlarged culturing and becomes secondary kind, three grades of kinds, the inoculum size volume ratio promptly: the upper level seed liquor is 5% with the volume ratio that is access in substratum; The Na of every grade of seed liquor wherein
2SeO
3Content is 5 μ g/ml; TPY substratum consisting of by weight wherein: 0.2 part of lactose, 0.2 part of casein peptone, 0.01 part of soy peptone, 0.01 part of extractum carnis, 0.01 part of yeast powder, Na
2HPO
40.01 part, KH
2PO
40.01 part, MgSO
40.005 part, 0.005 part of L-Cys, 0.001 part of Tween80.Carry out preparation of fermentation liquid: by the inoculum size volume ratio, i.e. the volume ratio of three grades of seed liquor and TPY fermented liquid: be 5%, three grades of seed liquor are inoculated in contain Na
2SeO
3In the TPY fermented liquid of 5 μ g/ml, 37 ± 1 ℃ of anaerobism are cultivated 7h; Medium centrifugal is concentrated, collect bacterium mud.Press bacterium mud: protective material=10: 1 (V/V) adds lyophilized vaccine, and thorough mixing is even.Place vacuum freeze drier then, pre-freeze starts vacuum to-40 ℃, carries out vacuum lyophilization under the vacuum condition of 0.1mmHg.Be lower than taking-up in 3% o'clock to bacterium powder moisture content, discharge vacuum, take out the viable bacteria powder that makes, pulverize the freeze-dried vaccine powder, cross 40 mesh sieves.Lyophilized vaccine consisting of by weight wherein: Vc is 0.1 part, and monosodium glutamate is 0.1 part, and skimming milk is 5 parts, and lactose is 5 parts.Pulverize the viable bacteria powder, sieve, medicinal powder preparation and packing.
The above-mentioned bifidus longum bb that utilizes is mixed with capsule preparations, by weight to the bacterium powder that method obtained that selenium carries out bio-transformation, its component is: the rich selenium bacterium of bifidus bacillus powder viable bacteria powder is more than 2 parts, and Magnesium Stearate is 0.2 part, and lactose is 20 parts, starch is 40 parts, and oligomeric isomaltose is 10 parts.
With the above-mentioned bacterium powder that method obtained that utilizes bifidus longum bb selenium to be carried out bio-transformation, be mixed with tablet formulation, by weight, its component is: the rich selenium bacterium powder of bifidus bacillus (viable bacteria powder) is more than 5 parts, and lactose is 20 parts, and glucose is 15 parts, Magnesium Stearate is 0.2 part, Microcrystalline Cellulose is 5 parts, and sodium starch glycolate is 4 parts, and micropowder silica gel is 0.01 part.
The above-mentioned bifidus longum bb that utilizes to the bacterium powder that method obtained that selenium carries out bio-transformation, is mixed with powder or granule, and by weight, its component is: (the viable bacteria powder is more than 2 parts, and lactose is 75 parts, and micropowder silica gel is 0.01 part for the rich selenium bacterium of bifidus bacillus powder.
Embodiment 2: at first with the bifidobacterium adolescentis inoculation in the TPY liquid nutrient medium, the volume ratio of inoculum size is that 12%, 37 ± 1 ℃ of anaerobism is cultivated 16h, is the one-level kind; Again it is carried out enlarged culturing and becomes secondary kind, three grades of kinds, the inoculum size volume ratio promptly: the upper level seed liquor is 12% with the volume ratio that is access in substratum; The Na of every grade of seed liquor wherein
2SeO
3Content is 15 μ g/ml; TPY substratum consisting of by weight wherein: 5 parts of lactose, 5 parts of casein peptones, 5 parts of soy peptones, 5 parts of extractum carniss, 5 parts of yeast powders, Na
2HPO
41.0 part, KH
2PO
41.0 part, MgSO
40.4 part, 0.2 part of L-Cys, 0.1 part of Tween80.Carry out preparation of fermentation liquid: by the inoculum size volume ratio, i.e. the volume ratio of three grades of seed liquor and TPY fermented liquid: be 12%, three grades of seed liquor are inoculated in contain Na
2SeO
3In the TPY fermented liquid of 15 μ g/ml, 37 ± 1 ℃ of anaerobism are cultivated 15h; Medium centrifugal is concentrated, collect bacterium mud.Press bacterium mud: protective material=30: 1 (V/V) adds lyophilized vaccine, and thorough mixing is even.Place vacuum freeze drier then, pre-freeze starts vacuum to-40 ℃, carries out vacuum lyophilization under the vacuum condition of 0.1mmHg.Be lower than taking-up in 3% o'clock to bacterium powder moisture content, discharge vacuum, take out the viable bacteria powder that makes, pulverize the freeze-dried vaccine powder, cross 40 mesh sieves.Lyophilized vaccine consisting of by weight wherein: Vc is 4 parts, and monosodium glutamate is 4 parts, and skimming milk is 20 parts, and lactose is 20 parts.Pulverize the viable bacteria powder, sieve, medicinal powder preparation and packing.
The above-mentioned bifidobacterium adolescentis that utilizes is mixed with capsule preparations, by weight to the bacterium powder that method obtained that selenium carries out bio-transformation, its component is: the rich selenium bacterium of bifidus bacillus powder viable bacteria powder is more than 2 parts, and Magnesium Stearate is 0.5 part, and lactose is 40 parts, starch is 60 parts, and oligomeric isomaltose is 30 parts.
With the above-mentioned bacterium powder that method obtained that utilizes bifidobacterium adolescentis selenium to be carried out bio-transformation, be mixed with tablet formulation, by weight, its component is: the rich selenium bacterium powder of bifidobacterium adolescentis (viable bacteria powder) is more than 5 parts, and lactose is 30 parts, and glucose is 25 parts, Magnesium Stearate is 0.6 part, Microcrystalline Cellulose is 15 parts, and sodium starch glycolate is 8 parts, and micropowder silica gel is 0.1 part.
With the above-mentioned bacterium powder that method obtained that utilizes bifidobacterium adolescentis selenium to be carried out bio-transformation, be mixed with powder or granule, by weight, its component is: the rich selenium bacterium powder of bifidobacterium adolescentis (viable bacteria powder) is more than 2 parts, lactose is 95 parts, and micropowder silica gel is 0.1 part.
Embodiment 3: at first with the bifidumbacterium bifidum inoculation in the TPY liquid nutrient medium, the volume ratio of inoculum size is that 6.5%, 37 ± 1 ℃ of anaerobism is cultivated 12h, is the one-level kind; Again it is carried out enlarged culturing and becomes secondary kind, three grades of kinds, the inoculum size volume ratio promptly: the upper level seed liquor is 8.5% with the volume ratio that is access in substratum; The Na of every grade of seed liquor wherein
2SeO
3Content is 10 μ g/ml; TPY substratum consisting of by weight wherein: 1.25 parts of lactose, 1.25 parts of casein peptones, 1 part of soy peptone, 0.4 part of extractum carnis, 0.4 part of yeast powder, Na
2HPO
40.35 part, KH
2PO
40.35 part, MgSO
40.015 part, 0.0125 part of L-Cys, 0.01 part of Tween80.Carry out preparation of fermentation liquid: by the inoculum size volume ratio, i.e. the volume ratio of three grades of seed liquor and TPY fermented liquid: be 8.5%, three grades of seed liquor are inoculated in contain Na
2SeO
3In the TPY fermented liquid of 10 μ g/ml, 37 ± 1 ℃ of anaerobism are cultivated 11h; Medium centrifugal is concentrated, collect bacterium mud.Press bacterium mud: protective material=20: 1 (V/V) adds lyophilized vaccine, and thorough mixing is even.Place vacuum freeze drier then, pre-freeze starts vacuum to-40 ℃, carries out vacuum lyophilization under the vacuum condition of 0.1mmHg.Be lower than taking-up in 3% o'clock to bacterium powder moisture content, discharge vacuum, take out the viable bacteria powder that makes; Pulverize the freeze-dried vaccine powder, cross 40 mesh sieves.Lyophilized vaccine consisting of by weight wherein: Vc is 1 part, and monosodium glutamate is 1 part, and skimming milk is 11.5 parts, and lactose is 10 parts.Pulverize the viable bacteria powder, sieve, medicinal powder preparation and packing.
The above-mentioned bacterium powder that method obtained that utilizes bifidumbacterium bifidum selenium to be carried out bio-transformation, be mixed with capsule preparations, by weight, its component is: the rich selenium bacterium of bifidumbacterium bifidum powder viable bacteria powder is more than 2 parts, Magnesium Stearate is 0.35 part, lactose is 30 parts, and starch is 50 parts, and oligomeric isomaltose is 20 parts.
With the above-mentioned bacterium powder that method obtained that utilizes not tally bifidus longum bb selenium to be carried out bio-transformation, be mixed with tablet formulation, by weight, its component is: the rich selenium bacterium powder of bifidumbacterium bifidum (viable bacteria powder) is more than 5 parts, and lactose is 25 parts, and glucose is 20 parts, Magnesium Stearate is 0.4 part, Microcrystalline Cellulose is 10 parts, and sodium starch glycolate is 6 parts, and micropowder silica gel is 0.05 part.
With the above-mentioned bacterium powder that method obtained that utilizes bifidumbacterium bifidum selenium to be carried out bio-transformation, be mixed with powder or granule, by weight, its component is: the rich selenium bacterium powder of bifidumbacterium bifidum (viable bacteria powder) is more than 2 parts, lactose is 85 parts, and micropowder silica gel is 0.055 part.
Embodiment 4: at first with the bifidobacteria infantis inoculation in the TPY liquid nutrient medium, the volume ratio of inoculum size is that 3%, 37 ± 1 ℃ of anaerobism is cultivated 10h, is the one-level kind; Again it is carried out enlarged culturing and becomes secondary kind, three grades of kinds, the inoculum size volume ratio promptly: the upper level seed liquor is 7% with the volume ratio that is access in substratum; The Na of every grade of seed liquor wherein
2SeO
3Content is 8 μ g/ml; TPY substratum consisting of by weight wherein: 0.8 part of lactose, 0.8 part of casein peptone, 0.8 part of soy peptone, 0.3 part of extractum carnis, 0.3 part of yeast powder, Na
2HPO
40.3 part, KH
2PO
40.3 part, MgSO
40.01 part, 0.01 part of L-Cys, 0.01 part of Tween80.Carry out preparation of fermentation liquid: by the inoculum size volume ratio, i.e. the volume ratio of three grades of seed liquor and TPY fermented liquid: be 8%, three grades of seed liquor are inoculated in contain Na
2SeO
3In the TPY fermented liquid of 8 μ g/ml, 37 ± 1 ℃ of anaerobism are cultivated 15h; Medium centrifugal is concentrated, collect bacterium mud.Press bacterium mud: protective material=18: 1 (V/V) adds lyophilized vaccine, and thorough mixing is even.Place vacuum freeze drier then, pre-freeze starts vacuum to-40 ℃, carries out vacuum lyophilization under the vacuum condition of 0.1mmHg.Be lower than taking-up in 3% o'clock to bacterium powder moisture content, discharge vacuum, take out the viable bacteria powder that makes, pulverize the freeze-dried vaccine powder, cross 40 mesh sieves.Lyophilized vaccine consisting of by weight wherein: Vc is 0.8 part, and monosodium glutamate is 0.8 part, and skimming milk is 10 parts, and lactose is 12 parts.Pulverize the viable bacteria powder, sieve, medicinal powder preparation and packing.
The above-mentioned bacterium powder that method obtained that utilizes bifidobacteria infantis selenium to be carried out bio-transformation, be mixed with capsule preparations, by weight, its component is: the rich selenium bacterium of bifidobacteria infantis powder viable bacteria powder is more than 5 parts, Magnesium Stearate is 0.3 part, lactose is 25 parts, and starch is 55 parts, and oligomeric isomaltose is 25 parts.
With the above-mentioned bacterium powder that method obtained that utilizes bifidobacteria infantis selenium to be carried out bio-transformation, be mixed with tablet formulation, by weight, its component is: the rich selenium bacterium powder of bifidobacteria infantis (viable bacteria powder) is more than 5 parts, and lactose is 25 parts, and glucose is 18 parts, Magnesium Stearate is 0.35 part, Microcrystalline Cellulose is 8.5 parts, and sodium starch glycolate is 7 parts, and micropowder silica gel is 0.06 part.
With the above-mentioned bacterium powder that method obtained that utilizes bifidobacteria infantis selenium to be carried out bio-transformation, be mixed with powder or granule, by weight, its component is: the rich selenium bacterium powder of bifidobacteria infantis (viable bacteria powder) is more than 2 parts, lactose is 80 parts, and micropowder silica gel is 0.08 part.
Embodiment 5: at first with the bifidus longum bb inoculation in the TPY liquid nutrient medium, the volume ratio of inoculum size is that 7%, 37 ± 1 ℃ of anaerobism is cultivated 16h, is the one-level kind; Again it is carried out enlarged culturing and becomes secondary kind, three grades of kinds, the inoculum size volume ratio promptly: the upper level seed liquor is 8% with the volume ratio that is access in substratum; The Na of every grade of seed liquor wherein
2SeO
3Content is 10 μ g/ml; TPY substratum consisting of by weight wherein: 1 part of lactose, 1 part of casein peptone, 0.5 part of soy peptone, 0.5 part of extractum carnis, 0.5 part of yeast powder, Na
2HPO
40.25 part, KH
2PO
40.25 part, MgSO
40.01 part, 0.05 part of L-Cys, 0.025 part of Tween80.Carry out preparation of fermentation liquid: by the inoculum size volume ratio, i.e. the volume ratio of three grades of seed liquor and TPY fermented liquid: be 9%, three grades of seed liquor are inoculated in contain Na
2SeO
3In the TPY fermented liquid of 10 μ g/ml, 37 ± 1 ℃ of anaerobism are cultivated 12h; Medium centrifugal is concentrated, collect bacterium mud.Press bacterium mud: protective material=20: 1 (V/V) adds lyophilized vaccine, and thorough mixing is even.Place vacuum freeze drier then, pre-freeze starts vacuum to-40 ℃, carries out vacuum lyophilization under the vacuum condition of 0.1mmHg.Be lower than taking-up in 3% o'clock to bacterium powder moisture content, discharge vacuum, take out the viable bacteria powder that makes, pulverize the freeze-dried vaccine powder, cross 40 mesh sieves.Lyophilized vaccine consisting of by weight wherein: Vc is 1 part, and monosodium glutamate is 1 part, and skimming milk is 12 parts, and lactose is 8 parts.Pulverize the viable bacteria powder, sieve, medicinal powder preparation and packing.
The above-mentioned bifidus longum bb that utilizes is mixed with capsule preparations, by weight to the bacterium powder that method obtained that selenium carries out bio-transformation, its component is: the rich selenium bacterium of bifidus bacillus powder viable bacteria powder is 5 parts, and Magnesium Stearate is 0.4 part, and lactose is 30 parts, starch is 50 parts, and oligomeric isomaltose is 20 parts.
With the above-mentioned bacterium powder that method obtained that utilizes bifidus longum bb selenium to be carried out bio-transformation, be mixed with tablet formulation, by weight, its component is: the rich selenium bacterium powder of bifidus bacillus (viable bacteria powder) is 10 parts, and lactose is 25 parts, and glucose is 22 parts, Magnesium Stearate is 0.48 part, Microcrystalline Cellulose is 10 parts, and sodium starch glycolate is 6 parts, and micropowder silica gel is 0.07 part.
The above-mentioned bifidus longum bb that utilizes to the bacterium powder that method obtained that selenium carries out bio-transformation, is mixed with powder or granule, and by weight, its component is: rich selenium bacterium powder (the viable bacteria powder) 2 parts of bifidus bacillus, and lactose is 90 parts, micropowder silica gel is 0.07 part.
Embodiment 6: at first with the bifidus longum bb inoculation in the TPY liquid nutrient medium, the volume ratio of inoculum size is that 0.5%, 37 ± 1 ℃ of anaerobism is cultivated 8h, is the one-level kind; Again it is carried out enlarged culturing and becomes secondary kind, three grades of kinds, the inoculum size volume ratio promptly: the upper level seed liquor is 5% with the volume ratio that is access in substratum; The Na of every grade of seed liquor wherein
2SeO
3Content is 5 μ g/ml; TPY substratum consisting of by weight wherein: 2.6 parts of lactose, 2.6 parts of casein peptones, 2.5 parts of soy peptones, 1.3 parts of extractum carniss, 0.4 part of yeast powder, Na
2HPO
40.35 part, KH
2PO
40.35 part, MgSO
40.015 part, 0.0125 part of L-Cys, 0.05 part of Tween80.Carry out preparation of fermentation liquid: by the inoculum size volume ratio, i.e. the volume ratio of three grades of seed liquor and TPY fermented liquid: be 10%, three grades of seed liquor are inoculated in contain Na
2SeO
3In the TPY fermented liquid of 5 μ g/ml, 37 ± 1 ℃ of anaerobism are cultivated 7h; Medium centrifugal is concentrated, collect bacterium mud.Press bacterium mud: protective material=20: 1 (V/V) adds lyophilized vaccine, and thorough mixing is even.Place vacuum freeze drier then, pre-freeze starts vacuum to-40 ℃, carries out vacuum lyophilization under the vacuum condition of 0.1mmHg.Be lower than taking-up in 3% o'clock to bacterium powder moisture content, discharge vacuum, take out the viable bacteria powder that makes, pulverize the freeze-dried vaccine powder, cross 40 mesh sieves.Lyophilized vaccine consisting of by weight wherein: Vc is 2.05 parts, and monosodium glutamate is 2.05 parts, and skimming milk is 12.5 parts, and lactose is 12.5 parts.Pulverize the viable bacteria powder, sieve, medicinal powder preparation and packing.
The above-mentioned bifidus longum bb that utilizes is mixed with capsule preparations, by weight to the bacterium powder that method obtained that selenium carries out bio-transformation, its component is: the rich selenium bacterium of bifidus bacillus powder viable bacteria powder is more than 2 parts, and Magnesium Stearate is 0.2 part, and lactose is 20 parts, starch is 40 parts, and oligomeric isomaltose is 10 parts.
With the above-mentioned bacterium powder that method obtained that utilizes bifidus longum bb selenium to be carried out bio-transformation, be mixed with tablet formulation, by weight, its component is: the rich selenium bacterium powder of bifidus bacillus (viable bacteria powder) is more than 5 parts, and lactose is 20 parts, and glucose is 15 parts, Magnesium Stearate is 0.2 part, Microcrystalline Cellulose is 5 parts, and sodium starch glycolate is 4 parts, and micropowder silica gel is 0.01 part.
The above-mentioned bifidus longum bb that utilizes to the bacterium powder that method obtained that selenium carries out bio-transformation, is mixed with powder or granule, and by weight, its component is: (the viable bacteria powder is more than 2 parts, and lactose is 75 parts, and micropowder silica gel is 0.01 part for the rich selenium bacterium of bifidus bacillus powder.
Embodiment 7:
As follows by the effect assessment of the rich selenium probiotics of the bifidus bacillus of the foregoing description gained after the cancer patients is edible: one, the effect assessment of the rich selenium probiotics of bifidus bacillus after 100 many cases Patients with Primary are edible:
The rich selenium probiotics of bifidus bacillus was taken 1---2 month as the food of Patients with Primary, compare with the patient who does not eat this preparation, that mostly the patient is showed is weak, apocleisis, abdominal distension, feel sick, big belly is painful, symptoms such as gastrointestinal dysfunction be improved significantly, the mental status takes a turn for the better.Wherein about 70% edible for patients said preparation after 2 months alpha-fetoprotein AFP obviously reduce, the knurl body is in steady state and no longer increases; About 10% edible for patients is after 4 months, and abdominal distension, symptom such as weak, nauseating disappear substantially, and TTPV disappears substantially.
Two: the one hundred rich selenium probiotics of the edible bifidus bacillus of many cases patients with lung cancer observation on effect:
As the food of patients with lung cancer, 100 many cases patients with lung cancer are after edible above-mentioned preparation 1--2 month, and first symptoms such as the cough that most patient is showed, bloody sputum, pectoralgia, heating all obtain obvious improvement, are emotionally stable, and the mental status is good.The edible said preparation of the patients with lung cancer that amynologic index CEA increases is after 2 months, and about 90% patient CEA drops near normal or normal level, and cancer cells is in the dormant stage, and the knurl body is in steady state, no longer increases.About 25% edible for patients said preparation is after 3 months, and first symptom of lung cancer such as cough, bloody sputum, pectoralgia, heating disappears substantially, and hydrothorax obviously reduces or disappears, and expiratory dyspnea alleviates, and the knurl body dwindles, blur center, sharpness of border.After more being apparent that part edible for patients said preparation, lump disappears substantially in the lung, reaches trivial bone superior gluteal lymph node in the lung and disappears.
Three, about tens routine cancer of the stomach volunteers ate the rich selenium probiotics of bifidus bacillus 1---2 month before the art first, and gastroscopic biopsy before the art and postoperative Pathologic specimen are carried out HE dyeing and CD34 monoclonal antibody immunohistochemical study, and set up edible the group to contrast.The result shows: edible group postoperative Pathologic specimen CD34 capillary blood vessel counting significantly is lower than the preceding gastroscope biopsy specimen of art, equally also is lower than edible group gastroscopic biopsy sample.
Four, the rich selenium probiotics of 50 many cases esophagus cancer edible for patients bifidus bacilluss is after February, and symptoms such as patient's malaise, poor appetite, abdominal distension are felt sick, gastrointestinal dysfunction are obviously improved, and the mental status takes a turn for the better.Wherein about 70% edible for patients said preparation blocked sense and alleviates after 2 months, and it is more smooth to take food.About 10% edible for patients blocked sense and all disappears after 3 months, and feed is normal.
Claims (11)
1, utilizes bifidus bacillus to the method that selenium carries out bio-transformation, it is characterized in that, include the seed liquor preparation that contains selenium; Fermentation; Centrifugal collection bacterium; The bacterium mud lyophilize that centrifugal collection bacterium is obtained obtains the viable bacteria powder; Pulverize above gained viable bacteria powder, sieve, above gained viable bacteria powder and auxiliary material mixed preparing and packing;
(1), contain the seed liquor preparation of selenium: bifidobacterium strains is inoculated in the TPY liquid nutrient medium, and wherein the volume ratio of inoculum size is 0.5%-12%, and 37 ± 1 ℃ of anaerobism are cultivated 8-16h, are the one-level kind; Again it is carried out enlarged culturing and become secondary kind, three grades of kinds, the volume ratio of inoculum size is 5%-12%, and wherein the volume ratio of inoculum size promptly: upper level seed liquor and the volume ratio that is access in substratum; The Na of every grade of seed liquor wherein
2SeO
3Content is 5-15 μ g/ml;
Wherein said TPY substratum consisting of by weight:
Lactose 0.2-5.0
Casein peptone 0.2-5.0
Soy peptone 0.01-5.0
Extractum carnis 0.01-5.0
Yeast powder 0.01-5.0
Na
2HPO
4 0.01-1.0
KH
2PO
4 0.01-1.0
MgSO
4 0.005-0.4
L-Cys 0.005-0.2
Tween80 0.001-0.1；
(2) preparation of fermentation liquid: the volume ratio by inoculum size is 5%-12%, and wherein the volume ratio of inoculum size is the volume ratio of three grades of seed liquor and TPY fermented liquid, three grades of seeds is inoculated in contain Na
2SeO
3In the TPY fermented liquid of 5-15 μ g/ml, 37 ± 1 ℃ of anaerobism are cultivated 7-15h;
(3), centrifugal collection bacterium: medium centrifugal concentrates, and collects bacterium mud;
(4), bacterium mud lyophilize that centrifugal collection bacterium is obtained obtains the viable bacteria powder, contains selenium and is higher than 50 μ g/g, the bifidobacteria viable bacteria number is higher than 1,000 hundred million/g; Wherein lyophilize: by volume with lyophilized vaccine and bacterium mud:
Bacterium mud: protective material=10-30: 1V/V adds lyophilized vaccine, and thorough mixing is even, carries out vacuum lyophilization, is lower than taking-up in 3% o'clock to bacterium powder moisture content; Pulverize the freeze-dried vaccine powder, cross 40 mesh sieves;
Lyophilized vaccine consisting of by weight wherein:
Vc 0.1-4.0
Monosodium glutamate 0.1-4.0
Skimming milk 5-20
Lactose 5-20;
(5), pulverize the viable bacteria powder, sieve, medicinal powder preparation and packing.
2, according to claim 1ly utilize bifidus bacillus, it is characterized in that described bifidus bacillus is bifidobacterium adolescentis, bifidumbacterium bifidum, bifidobacteria infantis or bifidus longum bb the method that selenium carries out bio-transformation.
3, according to claim 1ly a kind ofly utilize bifidus bacillus, it is characterized in that described TPY substratum composition by weight is the method that selenium carries out bio-transformation:
Lactose 1
Casein peptone 1
Soy peptone 0.5
Extractum carnis 0.5
Yeast powder 0.5
Na
2HPO
4 0.25
KH
2PO
4 0.25
MgSO
4 0.01
L-Cys 0.05
Tween80 0.025。
4, according to claim 1ly a kind ofly utilize bifidus bacillus, it is characterized in that, contain in the seed liquor preparation of selenium, wherein the Na of every grade of seed liquor the method that selenium carries out bio-transformation
2SeO
3Content is 10 μ g/ml.
5, according to claim 1ly a kind ofly utilize bifidus bacillus to the method that selenium carries out bio-transformation, it is characterized in that described lyophilized vaccine is Vc by weight: monosodium glutamate: skimming milk: lactose is 1: 1: 12: 8.
6, according to claim 1ly utilize bifidus bacillus to the product that selenium carries out bio-transformation, it is characterized in that, utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with capsule preparations, by weight, its component is:
The rich selenium bacterium of bifidus bacillus powder-viable bacteria powder is more than 2
Magnesium Stearate 0.2-0.5
Lactose 20-40
Starch 40-60
Oligomeric isomaltose 10-30.
7, according to claim 6ly utilize bifidus bacillus to the product that selenium carries out bio-transformation, it is characterized in that, utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with capsule preparations, by weight, component is:
The rich selenium bacterium of bifidus bacillus powder-viable bacteria powder 5
Magnesium Stearate 0.4
Lactose 30
Starch 50
Oligomeric isomaltose 20.
8, according to claim 1ly utilize bifidus bacillus to the product that selenium carries out bio-transformation, it is characterized in that, utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with tablet formulation, by weight, its component is:
The rich selenium bacterium of bifidus bacillus powder-viable bacteria powder is more than 5
Lactose 20-30
Glucose 15-25
Magnesium Stearate 0.2-0.6
Microcrystalline Cellulose 5-15
Sodium starch glycolate 4-8
Micropowder silica gel 0.01-0.1.
9, according to claim 8ly utilize bifidus bacillus to the product that selenium carries out bio-transformation, it is characterized in that, utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation with above-mentioned, be mixed with tablet formulation, by weight, its component is:
The rich selenium bacterium of bifidus bacillus powder-viable bacteria powder 10
Lactose 25
Glucose 22
Magnesium Stearate 0.48
Microcrystalline Cellulose 10
Sodium starch glycolate 6
Micropowder silica gel 0.07.
10, the product that utilizes bifidus bacillus selenium to be carried out bio-transformation according to claim 1, it is characterized in that, utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation, be mixed with powder or granule above-mentioned, by weight, its component is:
The rich selenium bacterium of bifidus bacillus powder-viable bacteria powder is more than 2
Lactose 75-95
Micropowder silica gel 0.01-0.1.
11, the product that utilizes bifidus bacillus selenium to be carried out bio-transformation according to claim 10, it is characterized in that, utilize bifidus bacillus to the bacterium powder that method obtained that selenium carries out bio-transformation, be mixed with powder or granule above-mentioned, by weight, its component is:
The rich selenium bacterium of bifidus bacillus powder-viable bacteria powder 2
Lactose 90
Micropowder silica gel 0.07.
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| CN109757730A (en) * | 2019-01-31 | 2019-05-17 | 山东环亿生物科技有限公司 | A kind of composition and preparation method thereof with reducing blood lipid, blood pressure and blood glucose |
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| CN100400649C (en) * | 2006-05-12 | 2008-07-09 | 河北农业大学 | Composite protectant for freeze dried fermenting prepn of directly thrown bifidobacterium |
| CN101606552B (en) * | 2009-05-06 | 2013-09-11 | 任发政 | Bifidobacterium deep freezing direct vat starter culture and composite cryoprotectant thereof |
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| CN107772477A (en) * | 2017-11-27 | 2018-03-09 | 内蒙古普泽生物制品有限责任公司 | A kind of compound probiotic powder preparation rich in selenium and preparation method thereof |
| CN109576176A (en) * | 2018-12-07 | 2019-04-05 | 江苏靶标生物医药研究所有限公司 | A kind of nanometer selenium bifidobacterium longum and its preparation method and application |
| CN109486715B (en) * | 2018-12-07 | 2022-07-15 | 江苏靶标生物医药研究所有限公司 | Selenium-enriched bifidobacterium longum and preparation method and application thereof |
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| CN112195147A (en) * | 2020-11-05 | 2021-01-08 | 苏州微克生活科技有限公司 | Method for cultivating high-activity bifidobacterium bifidum |
| CN112391318B (en) * | 2020-11-25 | 2022-07-01 | 广州态和生物科技有限公司 | Preparation method of bifidobacterium longum and selenium-enriched microorganism and preparation method of freeze-dried preparation thereof |
| CN116059256B (en) * | 2022-08-19 | 2023-11-14 | 广州态和生物科技有限公司 | Application of bifidobacterium longum and selenium-enriched microorganism in preparation of tumor prevention and treatment composition |
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| CN109757730A (en) * | 2019-01-31 | 2019-05-17 | 山东环亿生物科技有限公司 | A kind of composition and preparation method thereof with reducing blood lipid, blood pressure and blood glucose |
| CN109757730B (en) * | 2019-01-31 | 2021-06-04 | 山东环亿生物科技有限公司 | Composition with functions of reducing blood fat, blood pressure and blood sugar and preparation method thereof |
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