CN1267107C - Extraction method of scorpion active protein liquid - Google Patents
Extraction method of scorpion active protein liquid Download PDFInfo
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- CN1267107C CN1267107C CNB031264557A CN03126455A CN1267107C CN 1267107 C CN1267107 C CN 1267107C CN B031264557 A CNB031264557 A CN B031264557A CN 03126455 A CN03126455 A CN 03126455A CN 1267107 C CN1267107 C CN 1267107C
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Abstract
The present invention relates to an extraction method of an active protein secretion liquid of living scorpion, which is applied to protein extraction in the production of medicines. Firstly, living scorpion is disinfected; the needle head of an injector is inserted into the protein bag at the middle position of the fifth section of the post abdomen in the scorpion to extract raw liquids before protein secretion, during protein secretion and after protein secretion of the living scorpion; then, the digestive juice extracted from the self body, the internal body and the external body of the living scorpion is added into the raw liquids of protein secretion of the living scorpion for separation and extraction. The protein secretion liquid obtained by the method is high in activity, and the operation is simple and convenient. The protein secretion liquid contains various amino acids, crude proteins and rich trace elements required by human bodies. The protein secretion liquid can be prepared into a wine base or water base oral liquid of a certain proportion so that the shelf life of active protein can be prolonged. The protein secretion liquid can also be directly used for treating various diseases without toxic or side effect.
Description
Technical field
The present invention relates to a kind of extracting method of active animal protein juice, particularly a kind of production and extracting method of the scorpion activated protein juice of living.
Background technology
Scorpio is the traditional rare Chinese medicine of China, and people just recognize that Scorpio is the good medicine of curing the disease before bimillennium, and have put into clinical practice.Song's " Kaibao Bencao " beginning is with the title of " Scorpio ", in the bright Compendium of Material Medica form of Scorpio, purposes etc. all done detailed description.In " Traditional Chinese Medicine dictionary " the record Scorpio have endogenous wind stopping pain relieving, dredge the meridian passage, activating blood circulation to dissipate blood stasis, dispersing pathogen accumulation, logical muscle deep, by the wet effect such as wind of removing.Chinese patent medicine with the Scorpio preparation has reached kind more than 30, as treat " nantong sheyao " and some specific drugs of prosopoplegic " QIANZHENGSAN ", treatment snakebite, as " reproduce nine, DAHUOLUO DAN, seven precious red, Zhijing San ... " Scorpio can also make more than 70 kind of prescription with other Chinese medicine.Treat diseases such as acute and chronic infantile convulsion, migraine, arthralgia, tetanus, hypertension, toothache, arteriosclerosis.In recent years, Scorpio was developed into edible health, put forward frontier such as poison and became one of China's outlet medical material from simple medication.
By discovering, nutrition that Scorpio is real and medical value are the intravital activated protein of Scorpio, and its effect is tens times of traditional Scorpio medical material.But China all is the mode of taking Scorpio to be used as medicine to Scorpio for a long time, and this mode is low to the utilization rate of Scorpio medical value, effect is slow.Such as, the common way of people is to become medicated wine to take the Scorpio infusion, this method can only leaching goes out the activated protein about 3%, so the utilization rate of effective ingredient is very low.Scorpion live simultaneously not only bad for transportation and processing, because of life habit and the toxicity of Scorpio also can produce certain danger to the people.As the activated protein juice that provides a kind of method can directly extract Scorpio, just can solve the problem of above-mentioned existence, improve the medical value of Scorpio.
Discover that simultaneously the intravital pharmaceutical protein overwhelming majority of Scorpio concentrates in its intravital several albumen sacs, those albumen sacs connect blood circulation, digestive system and excretory system etc. by pipeline.Protein liquid in those albumen sacs is the place of a centralized stores of Scorpio body protein excretion liquid, and then is transported to whole body thus.The method of traditional extraction Scorpio protein liquid is that Scorpio is dissected, and dissects the back and seeks secreting gland, and obtain this albumen sac from secreting gland, obtains protein liquid stock solution.But this extracting method is too complicated, and extracted amount is few and protein liquid activity that obtain is lower.
The activity of scorpion activated protein is very strong though live, and after leaving the Scorpio body, memory time is very short, general only within several hrs its activity will lose.And after its active extracting section of tool come out, be mixed with proportion of liquid with other base fluids, just can realize keeping for a long time its activity.
Summary of the invention
In order to improve the utilization rate of this rare medicinal herbs of Scorpio, the invention provides a kind of extracting method of the scorpion activated protein of living, it is not only easy and simple to handle, and the activity of protein liquid is also higher.
Through discovering, the position of protein excretion capsule is fixed in the body of Scorpio live body adult, there is an albumen sac the 5th 60% position of saving, centre that wherein is positioned at the postabdomen of Scorpio live body, protein liquid after the secretion, protein liquid and the not excretory protein liquid in the secretion are arranged in this albumen sac, and this albumen sac is Long Circle, and front end has three secreting glands, there are two secreting glands the rear end, use syringe just can directly insert in this albumen sac, extract inner protein excretion liquid out, exempt the trouble of dissecting one by one.
The extracting method of scorpion activated protein juice alive of the present invention, the protein liquid that is applied in medicine and the food production extracts, and comprises the following steps:
(1) live scorpion sterilization: the scorpion spraying disinfection alives that at first adopts alcohol content desire to be extracted greater than 50% Chinese liquor, reuse wiping disinfectant method is wiped postabdomen the work scorpion with alcohol content greater than 50% Chinese liquor, and sterilization carries out disinfection;
(2) extract to live in the scorpion protein excretion capsule in the protein excretion, before the secretion and the protein excretion stock solution after the secretion: the syringe needle of small syringe is inserted the body protein excretion capsule in the centre position that is positioned at scorpion postabdomen the 5th joint alive, extracts protein excretion stock solution out;
(3) isolate ABC type protein excretion liquid: in the above-mentioned scorpion activated protein alive secretion stock solution that obtains, add the scorpion alive of extracting in body, body, the external digestion juice, isolate the protein liquid of different levels after cold preservation is static respectively, layering is extracted and is obtained A, B, C type activated protein juice.
After syringe needle inserts the Scorpio postabdomen, observe the reflection of the back tailpin of its afterbody, when the tailpin of its postabdomen trembles fast, just begin to extract protein excretion stock solution, the liquid of extraction is sky blue translucent.
During stock solution in the scorpion activated protein secretory sac of extract living, compel the preabdomen of scorpion alive to weight, then push scorpion abdominal part alive more from front to back, push while extracting by light.
Separate in A type protein excretion liquid 2 hours after extracting activated protein secretion stock solution out and carry out, in the activated protein secretion stock solution of extracting out, add the scorpion alive of extracting in body, body, the external digestion juice, fully shake up, static 2 hours of 0～3 degree centigrade of cold preservation, activated protein secretion stock solution begins layering, extract supernatant, obtain A type scorpion protein excretion alive liquid, obtain Type B and C type scorpion protein excretion alive liquid at other layer simultaneously.
Above-mentioned scorpion alive in body, body, the external digestion juice is to extract like this: the 3rd joint centre position before the scorpion gonopore of living, with the direct inserting needle of syringe, parallel backward insertion extract inner in body, body, the external digestion juice, during extraction while extracting from after regular forward pushing.
Above-mentioned albumen stock solution extracts the back and separates and precedingly can also comprise concentrated, the preservation of protein excretion liquid and the processing procedure of thawing, and may further comprise the steps:
(1) concentrate quick-freezing: the scorpion protein excretion stock solution alive that will extract concentrates the scanning frequency of going forward side by side to freeze 24 hours time;
(2) cold preservation preservation: with protein excretion stock solution cold preservation preservation in the time of-5～-10 degrees centigrade of quick-freezing, the preservation time can reach several years;
(3) thaw: wash away the container that holds protein excretion stock solution or it is thawed 0～3 degree centigrade the time with cold water, the albumen stock solution after thawing just can be carried out separating treatment.
The using method of A that above-mentioned separation obtains or Type B protein excretion is mixed with wine base or water base oral liquid with it, can be directly used in the treatment various diseases.
The present invention utilizes the method for direct extraction Scorpio live body protein excretion liquid to extract activated protein, and protein excretion stock solution productive rate height, active strong can be extracted the activated protein in the proteic secretory sac of Scorpio live body out fully; Owing to be the protein excretion liquid of directly extracting out from live body, it is active strong, produces the protein excretion liquid with greater activity through after the cryogenic separation again.Easy and simple to handle, improved the utilization rate of labor productivity and Scorpio activated protein greatly.The secretion stock solution of extracting in this albumen sac is the medicinal elite part of Scorpio.
The scorpion alive of extracting in body, body, the external digestion juice is the enzyme reactive protein, has higher pharmaceutical value, be that the treatment stomach rises and dyspeptic specially good effect activated protein, it has the effect of separating clarifying voluntarily with after the interior juice of scorpion albumen sac of living mixes.
The activated protein juice that utilizes said method to obtain can be mixed with the water base or wine base oral liquid of certain proportion with base fluids such as distilled water or Chinese liquor, this manner of formulation can make it still have higher activity after long-term storage, this oral liquid can directly be taken, with the treatment various diseases.
With said method make scorpion activated protein juice alive, contain the necessary several amino acids of multiple human body, crude protein and abundant trace element, wherein containing human body self can not synthetic eight kinds of essential amino acids.Contain a large amount of trace element-selenium, it has the function of anticancer growth.Through clinical trial, the scorpion albumen of finding to live has good reparation, activation and protective effect to human body cell; It can keep a weakly alkaline body fluid environment, and is favourable to the existence of probiotics, has destroyed the acid body fluid environment that harmful bacterium depends on for existence simultaneously, thereby has caused a kind of sick body constitution that is difficult for; It has integration, can integrate the intravital heavy metal ion of people, metabolic waste and other poisonous substances, and excrete; Its effective object is not limited to a certain organ of human body, but human body is had comprehensive regulating action.Take through a large amount of patients, prove that this product has comprehensive comprehensive function of the function of active cell, slow down aging, vessel softening, microcirculation improvement and enhancing human body immunity power and nutrition, adjusting, reparation to human body.
The specific embodiment
Embodiment: get 1000 of Scorpio live body adults, prepare Chinese liquor, the disinfecting cotton swab of one of 1 milliliter of microsyringe, spirituosity 55%, 150 milliliters of transparent vessels and refrigerator.
Chinese liquor with 55% is to the scorpion spraying disinfection of living, with same Chinese liquor the postabdomen of the scorpion that lives is carried out the wiping sterilization then, find the centre position of Scorpio postabdomen the 5th joint accurately, syringe needle with 1 milliliter microsyringe inserts appropriate depth in this position then, note observing the reaction of the tailpin that is positioned at the Scorpio afterbody during insertion, when the tailpin of finding Scorpio trembles fast, illustrate that syringe needle has inserted albumen sac.At this moment begin to extract the albumen stock solution in the albumen sac, during stock solution in extracting the scorpion activated protein secretory sac of living, compel the preabdomen of scorpion alive to weight, then push scorpion abdominal part alive more from front to back, push while extracting by light.It is sky blue that the protein excretion stock solution of extracting out is.
After finishing the protein excretion stock solution extraction of a Scorpio, carry out the extraction of the protein excretion stock solution of second Scorpio, finish until extraction by above-mentioned step.
The syringe volume is put into transparent vessel with protein excretion stock solution after taking out and expiring, and this transparent vessel put into refrigerator cold preservation, once more protein excretion stock solution is put into transparent vessel after taking out full syringe, finish the extraction of protein excretion stock solution one by one, above-mentioned process probably obtains about 100 milliliters sky blue protein excretion stock solution.
Because this protein excretion stock solution contains multiple material, ingress of air is extremely apt to deteriorate and lose activity in non-living body, therefore the protein excretion stock solution that obtains can't directly be carried out the routine preservation and be taken, and also must further purify and preparation, could preserve at normal temperatures than the long time.
The main effective ingredient of the activated protein of Scorpio secretion stock solution is a water-solubility protein, and separations of therefore can water purifying has water-disintegrablely owing to activated protein simultaneously, should avoid making water to extract as far as possible.
100 milliliters of above-mentioned protein excretion stock solutions are separated, at first the scorpion alive of in this protein excretion stock solution of 100 milliliters, add extracting in body, body, the external digestion juice, this digestive fluid is to extract like this: the 3rd joint centre position before the scorpion gonopore of living, with the direct inserting needle of syringe, parallel backward insertion extract inner in body, body, the external digestion juice, during extraction while extracting from after regular forward pushing.And use the Digestive system of the scorpion alive of equal number to come the protein isolate juice.
Shake up after adding above-mentioned digestive fluid, static 2 hours of 0～3 degree centigrade of following cold preservation, at this moment the protein excretion stock solution in the container just is divided into two-layer, the upper strata is clarifying transparency liquid, lower floor is an emulsion, extract the supernatant liquid on top, this liquid is the highest active Scorpio protein excretion liquid, and we are called A type protein excretion liquid.
Also contain some activated protein in the emulsion of above-mentioned lower floor, the scorpion alive that in this emulsion, adds extracting section again in body, body, external digestive fluid, extract supernatant once more by above-mentioned steps, we are called Type B protein excretion liquid.Remainder can be called C type protein excretion liquid.
The activity of above-mentioned A type that obtains and Type B protein excretion liquid is stronger, but preservation is also difficult relatively, must low temperature or freezing preservation.Therefore, be necessary above-mentioned protein excretion liquid is processed into oral liquid or other medicines that contains base fluid.In Chinese liquor with 92 milliliters of 8 milliliters of addings of above-mentioned A type protein excretion liquid, make the oral liquid of content 8%, also Chinese liquor can be changed into distilled water and make water base oral liquid.Certainly, can prepare the oral liquid of variable concentrations according to the difference of consumption.As:
The wine base or the water base oral liquid that contain the activated protein juice 8%～10% that the inventive method produces can be treated cancerous pain, and its analgesic effect is 20 times of dolantin, 10 times of morphine.
The wine base or the water base oral liquid that contain the activated protein juice 5%～6% that the inventive method produces can be treated the asthma that is difficult to capture, and take behind this product all produce effects immediately, alleviate fully.
The wine base or the water base oral liquid that contain the activated protein juice 4%～5% of the inventive method production can be treated scapulohumeral periarthritis, and pain can be alleviated fully, do not produce into the interruption reaction.
The wine base or the water base oral liquid that contain the activated protein juice that the inventive method produces can be treated tumor, have the leukocyte killed after cancerous cell, the rising chemotherapy, improve immunity.
The activated protein juice that the inventive method is produced all has fabulous restitution for the limited and disease itself of limb function that the fracture equivalent damage causes; For the visual disturbance that optic atrophy, optical fundus blood vessel sclerosis etc. causes, very fast improvement effect is arranged at short notice after taking this product.
Through Beijing nutrient research institute Analysis and Identification, contain various trace elements such as several amino acids such as aspartic acid, threonine, serine, glutamic acid, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline and potassium, sodium, calcium, magnesium, ferrum, copper, zinc, selenium in this protein excretion liquid.
Through efficacy analysis to the oral liquid of this protein excretion liquid preparation, the total effective rate of various symptoms is reached 95%, take personnel 41 people of this product through investigation, wherein, scapulohumeral periarthritis 4 examples, cancer 5 examples, visual disorder 15 examples, psoriasis 3 examples, Bai Dianfeng 1 example, hypertension 3 examples, dermatosis 5 examples, lumbar muscle strain, fracture sequelae 5 examples, nervous system disease 5 examples, be reflected in 38 examples that took effect the same day after taking, account for 40 examples that take effect in 92.7%, 10 day, account for 97.6%.
By method of the present invention, can take out the activated protein of Scorpio live body very easily, and after purifying, be made into various oral liquids, be used to prolong the holding time of activated protein, or directly take with the treatment various diseases.
Claims (5)
1. the extracting method of scorpion activated protein juice of living is applied to the extraction of the scorpion activated protein juice alive in the medicine production, it is characterized in that: comprise the following steps:
(1) live scorpion sterilization: the scorpion spraying disinfection alives that at first adopts alcohol content desire to be extracted greater than 50% Chinese liquor, reuse wiping disinfectant method is wiped postabdomen the work scorpion with alcohol content greater than 50% Chinese liquor, and sterilization carries out disinfection;
(2) extract to live in the scorpion protein excretion capsule in the protein excretion, before the secretion and the protein excretion stock solution after the secretion: the syringe needle of small syringe is inserted the body protein excretion capsule in the centre position that is positioned at scorpion abdominal part the 5th joint alive, extracts protein excretion stock solution out;
(3) isolate ABC type protein excretion liquid: in the above-mentioned scorpion activated protein secretion stock solution alive that obtains, add the scorpion alive of extraction from body, in the body, external digestion liquid, described extraction is lived scorpion from body, in the body, the method of external digestion liquid is: the 3rd joint centre position before the scorpion gonopore of living, with the direct inserting needle of syringe, parallel backward insertion is extracted inner from body, in the body, external consumption juice, during extraction while extracting from after regular forward pushing, isolate the protein excretion liquid of different levels after cold preservation is static respectively, layering is extracted and is obtained A, B, C type activated protein juice.
2. the extracting method of scorpion activated protein juice alive according to claim 1, it is characterized in that: after syringe needle inserts the Scorpio postabdomen, observe the reaction of the back tailpin of its afterbody, when the tailpin of its postabdomen trembles fast, just begin to extract protein excretion stock solution, the liquid of extraction is sky blue translucent.
3. the extracting method of scorpion activated protein juice alive according to claim 1, it is characterized in that: during stock solution in the scorpion activated protein secretory sac of extract living, to then push scorpion abdominal part alive more from front to back by gently to the preabdomen of the urgent scorpion that lives of weight, push while extracting.
4. the extracting method of scorpion alive according to claim 1 scorpion protein excretion alive liquid, it is characterized in that: separate A type protein excretion liquid and be in 2 hours after extracting activated protein secretion stock solution out and carry out, in the activated protein secretion stock solution of extracting out, add the activity extracted in body, body, external digestion liquid, fully shake up, static 2 hours of 0-3 degree centigrade of cold preservation, activated protein secretion stock solution begins layering, extract supernatant, obtain A type scorpion protein excretion alive liquid, obtain Type B and C type scorpion protein excretion alive liquid at other layer simultaneously.
5. the extracting method of scorpion activated protein juice alive according to claim 1 is characterized in that: albumen stock solution extracts the back and separates and precedingly can also comprise concentrated, the preservation of protein excretion liquid and the processing procedure of thawing, and may further comprise the steps:
(1) concentrate quick-freezing: the scorpion protein excretion stock solution alive that will extract concentrates the scanning frequency of going forward side by side to freeze 24 hours time;
(2) cold preservation preservation: will be protein excretion stock solution cold preservation preservation in the time of-5--10 degree centigrade of quick-freezing, the preservation time can reach several years;
(3) thaw: wash away the container that holds protein excretion stock solution or it is thawed 0-3 degree centigrade the time with cold water, the albumen stock solution after thawing just can be carried out separating treatment.
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| CNB031264557A CN1267107C (en) | 2003-09-28 | 2003-09-28 | Extraction method of scorpion active protein liquid |
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| CNB031264557A CN1267107C (en) | 2003-09-28 | 2003-09-28 | Extraction method of scorpion active protein liquid |
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| CN1267107C true CN1267107C (en) | 2006-08-02 |
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| CN107501403A (en) * | 2017-10-09 | 2017-12-22 | 南京图艾生物医药科技有限公司 | A kind of extraction of scorpion toxin and purifying process |
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Granted publication date: 20060802 Termination date: 20190928 |