CN100548315C - Silkworm health-care product and preparation method thereof - Google Patents
Silkworm health-care product and preparation method thereof Download PDFInfo
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- CN100548315C CN100548315C CNB2006100247014A CN200610024701A CN100548315C CN 100548315 C CN100548315 C CN 100548315C CN B2006100247014 A CNB2006100247014 A CN B2006100247014A CN 200610024701 A CN200610024701 A CN 200610024701A CN 100548315 C CN100548315 C CN 100548315C
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Abstract
The invention provides with 5 health product that age, the silkworm preparation had treatment and prevent diabetes effect.Be specifically related to 5 age silkworm with preparation method such as protease digestion and by the related preparations of this method preparation.Said preparation is rich in the abundant nutrition material, contains necessary for human body aminoacid and effective blood sugar reducing component, and the therapeutic effect that the preventive effect and the hyperglycemia of hyperglycemia produced back (type ii diabetes) is good, is ideal blood sugar regulator.It is low, effective that the inventive method has a production cost, safety, taking convenience, advantage such as quality controllable.
Description
Technical field
The present invention relates to health product, be specifically related to have health product of treatment and prevent diabetes effect and preparation method thereof with silkworm preparation in 5 ages.
Background technology
Diabetes (Diabetes Mellitus, DM) be a kind of common chronic endocrine metabolism disease, principal character is hyperglycemia and glycosuria, the symptoms such as " three-many-one-littles " that shows as polydipsia, polyphagia, polyuria clinically and lose weight, and the traditional Chinese medical science is referred to as to quench one's thirst.
Diabetes are a kind of affluenza.In recent years, along with the raising of living standards of the people, the onset diabetes rate raises year by year.Type ii diabetes accounts for the 85%-95% of whole diabetes ratio in the world, and the sickness rate of China [is being seen Guo Xiao China etc., the type 2 diabetes mellitus rat model of high glucose and high fat diet induced and nephropathy characteristics thereof more than 90%, China's diabetes magazine, 2002,10 5 phases of volume, 290-294].Diabetes are the treatment of type ii diabetes and the important topic that prevention becomes the medical worker particularly.The folkd therapy of utilizing silkworm treatment diabetes is all arranged in China, Japan, Korea S.The record of " Pupa bombycis is fried in shallow oil the juice drink, only quenches one's thirst " is arranged in Compendium of Material Medica.It is the preparation method of healthy accesary foods of blood sugar lowering and silkworm powder with the silkworm powder that the effect that Korea S has treatment diabetes at silkworm has proposed, and applies for a patent (Chinese patent notification number 1127637) simultaneously in China, Japan, Korea S.Subsequently, state-owned how tame unit has also applied for the relevant patent of relevant silkworm powder preparation method in.But taking convenience is not arranged so far as yet, effective silkworm health-care product.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of and prepares taking convenience, safe, quality controllable silkworm health-care product with silkworm.
The invention provides a kind of silkworm health-care product with blood sugar regulation function.
The present invention seeks to realize like this:, use protease digestion, the separating digesting product with after 3 to 7 the 5 ages live body silkworm quick freezing.
The present invention has announced a kind ofly have hyperglycemia that the high sugared high protein diet of the high fat of prevention causes and the preparation method that type ii diabetes is had the health nutrient of therapeutic efficiency with protease digestion silkworm in 5 age preparation.
Material therefor of the present invention is as follows:
Silkworm: 871 * 872 He Su town * spring scenery, provide silkworm egg by Inst. of Silkworm, Chinese Academy of Agricultural Sciences, raise to 5 ages with Folium Mori.Pepsin (1: 250), trypsin 1: 250) protease, neutral protease, animal proteolytic enzyme (compound enzyme), cathepsin, papain, subtilisin etc. is market and sells product.Testing uric acid test kit (oxidation enzyme process) is available from Shanghai Rongsheng Bioisystech Co., Ltd.The glucose assays test kit is available from Shanghai Vaccine and Serum Institute.The laboratory animal kunming mouse is bought the Shanghai Experimental Animal Center in the Chinese Academy of Sciences.Other all chemical drugss are analytical pure.Tangential flow ultrafilter Vivaflow 50 is a vivascience company product, molecular cut off 5000.
Method of the present invention comprises the following steps:
The processing of silkworm (3 to 75 ages that will raise, the live body silkworm was packed in the plastic bag, and-20 ℃ of quick freezing are preserved) → pulverize (tissue pulverizer) → protease digestion → boil enzyme denaturing work → water phase separated (removal solid matter) → ultrafiltration (removal macromolecular substances) → quality testing → product product of the present invention to can be oral formulations such as oral liquid, capsule, tablet or granule.
The inventive method specifically has the following step:
(1) processing of silkworm:
3 days 5 ages silkworm to 7 day silkworm all can, the live body silkworm of raising is packed in the plastic bag ,-20 ℃ of quick freezing are preserved;
(2) protease digestion:
The silkworm of freezing preservation is taken out, weigh, by weight adding the deionization purified water for the ratio of 0.5-2, with organizing pulverizer to pulverize, add protease (pepsin, trypsin, protease, neutral protease, animal proteolytic enzyme (compound enzyme), cathepsin, papain, subtilisin etc. all can) digestion by the heavy 0.1-2% of silkworm, boil enzyme denaturing and live, cooling, pH2-5 is with 2 layers of filtered through gauze under acid condition, and extracts, and collects water;
(3) ultrafiltration
Supernatant after the above-mentioned filtration is transferred pH to 6-7 with NaOH, filter with tangential flow ultrafilter by peristaltic pump, remove indigested protein, fatty macromolecular substances, filtered solution is standby;
(4) drying:
The filtered solution obtained by freeze drying powder of above-mentioned steps (3) is standby;
(5) preparation:
The filtered solution of above-mentioned steps (3) or the powder of step (4) are made the silkworm health-care product preparation according to a conventional method.
Quality control
1), to the inhibition effect measuring of alpha-glucosaccharase enzyme activity
1. alpha-glucosidase preparation: put to death mice, get small intestinal then and extrude the food elk, intestinal wall is clean with normal saline flushing, scrape with slide and to get mucosa, pulverize behind the ice bath 5Min, be resuspended in (0.02M PBSPh7.2) in the phosphate buffer, 4 ℃ of centrifugal 10Min of 4000RPM are to remove big cell debris, continuation is got supernatant and is made alpha-glucosidase with 4 ℃ of centrifugal 20Min of 10000RPM.After ultraviolet method is surveyed protein concentration, standby in-20 ℃ of preservations.Add the phosphate buffer of 200 μ L 0.0067mol/L pH6.8 during use, be made into the 0.1mg/ml enzymatic solution.
2. alpha-glucosaccharase enzyme activity determination: 50 μ L enzymatic solution and 50 μ L water are mixed, 37 ℃ of pre-warm water enzymatic mixtures 5 minutes, add 100 μ L substrates (0.028M maltose/0.1M maleic acid pH6.8), 37 ℃ of water-baths 10 minutes add 40 μ lNa2CO3 (0.1mol/L) stopped reactions.Get 20 μ l solution glucose assays test kit survey glucose content wherein.With at pH6.8, temperature is that it is the unit of alpha-glucosaccharase enzyme activity that per minute hydrolysis maltose discharges the required enzyme amount of 1 μ mol glucose, calculates by following formula under 37 ℃ the condition:
Alpha-glucosaccharase enzyme activity (I/mg)=concentration of glucose (μ M) * 0.00024 ÷ 0.005 ÷ 10
When alpha-glucosaccharase enzyme activity (I/mg) 〉=10, can be used for the inhibition effect measuring of alpha-glucosaccharase enzyme activity
3. the inhibition effect measuring of alpha-glucosaccharase enzyme activity: 50 μ L enzymatic solution and 50 μ L water (in contrast) or 50 μ L health care liquid are mixed, 37 ℃ of pre-warm water enzymatic mixtures 5 minutes, add 100 μ L substrates (0.028M maltose/0.1M maleic acid pH6.8), 37 ℃ of water-baths 30 minutes add 40 μ lNa2CO3 (0.1mol/L) stopped reactions.Get 20 μ l solution glucose assays test kit survey glucose content wherein.Calculate suppression ratio by following formula:
Suppression ratio=(control tube glucose content-experiment tube glucose content) ÷ control tube glucose content * 100%
Quality control requirement: suppression ratio 〉=80%
2), aminoacid (polypeptide) assay
To keep healthy 100 times of liquid dilute with waters are blank with water, and ultraviolet scanner is measured the absorbance value of 260nm and 280nm, calculates aminoacid (polypeptide) content by following formula:
Content (mg/mL)=(1.47 * 280nm absorption value-0.47 * 260nm absorption value) * 100
Quality control requirement: content 〉=50mg/m L.
3), urate assay
Measure urate content with the testing uric acid test kit (oxidation enzyme process) that Shanghai Rongsheng Bioisystech Co., Ltd produces, also adding change (be about to reaction system and change 2mL into by 1mL) approximately with reference to description carries out: after promptly according to the form below adds related reagent, mixing, 37C water-bath 5min, in wavelength 520nm, with the blank zeroing, measure light absorption value.Uric acid content=(sample light absorption value/calibration tube light absorption value) * 6mg/L.，
| Sample tube (S) | Calibration tube (AL) | Blank (B) | |
| Distilled water | 50μL | ||
| Sample | 50μL | ||
| Calibration solution | 50μL |
| Reagent | 2.0mL | 2.0mL | 2.0mL |
Quality control requirement: uric acid content≤50mg/L
Product
Two kinds of products, one is the health care liquid of adjusting concentration after quality determination, two freeze-dried powders for health care liquid.
Health care liquid: sample is adjusted concentration after quality determination, behind the aseptic membrane filtration of 0.22 μ m, and packing, room temperature preservation.
Freeze-dried powder: through sample freezer dryer lyophilizing, packing, room temperature or the 4 ℃ of preservations after the quality testing.
With the lyophilizing health product of the inventive method preparation send Chinese Academy of Sciences Shanghai biochemical with the used acid cleavage of RESEARCH ON CELL-BIOLOGY after measure amino acid content with automatic amino acid analyzer, amino acid content is 36.64% in these health product as a result, and various amino acid whose compositions see the following form:
| Aminoacid | Millimole | ％ | Aminoacid | Millimole | ％ |
| Taurine TAU | Different bright Ile | 1.73 | |||
| Radix Asparagi Asp | 4.33 | Bright Leu | 2.60 | ||
| The Thr of Soviet Union | 1.59 | Cheese Tyr | 1.56 | ||
| Silk Ser | 1.49 | Phenylpropyl alcohol Phe | 1.93 | ||
| Paddy Glu | 5.41 | Bird ORN | 0.30 | ||
| Dried meat Pro | 0.70 | Rely Lys | 2.61 | ||
| Sweet Gly | 2.59 | Ammonia NH 3 | |||
| Third Ala | 2.32 | Group His | 1.54 | ||
| Guang Cys | 0.41 | Color Try | |||
| Figured silk fabrics Val | 2.36 | Smart Arg | 2.45 | ||
| First sulfur Met | 0.72 |
The silkworm health nutrient device that the inventive method makes is as follows through the zoopery test result:
Blood sugar lowering zoopery result with the health product of the inventive method preparation
One, to the blood sugar decreasing effect of type-II diabetes animal model Mus
Material: laboratory animal: male mouse of kunming (20g ± 2g), available from Medical Center of Fudan University.The glucose assays test kit is available from Shanghai Vaccine and Serum Institute; Alloxan (Alloxan) takes by weighing 0.210g available from Fluka company during use, use the 10ml physiological saline solution.The Remedies for diabetes glucobay (acarbose) is available from first pharmacy.The high glucose and high fat high protein feed: provide prescription by this laboratory, mouse feed (cookies) is made by INM animal feed factory.The prescription of this feedstuff is as follows: conventional feed 112.5kg, sugar 30kg, yolk powder: 4.5kg, Adeps Sus domestica 3kg.Health care liquid: with reference to the inventive method description preparation, its aminoacid (polypeptide) content is 60mg/mL, is 85.61% to the suppression ratio of glycosidase enzyme activity, and uric acid content is 29mg/L.
Method: 100 μ l prepare the alloxan diabetes mouse model by the normal mouse tail vein injection, behind injection 48h, survey fasting glucose, choose blood glucose value and divide into groups the above person of 200mg/dL, the difference of each treated animal average blood sugar value is not more than 10mg/dL during grouping, 9 every group.Be divided into 3 groups, the liquid experimental group that promptly keeps healthy grouping, medicine (glucobay (acarbose)) matched group, normal saline matched group.Raise with the high glucose and high fat high protein feed grouping back.Health care liquid and medicine are all used the drinking method feed, promptly observe earlier each treated animal amount of drinking water (about 200mL) of every day, health care liquid, medicine, normal saline with used dosage is diluted with water to 200mL respectively again, put into the drinking-water bottle, drink for mice, carry out scalding to used drinking-water bottle every day, changes liquid once.The dosage of health care liquid is 0.5mL/ Mus/sky, and the dosage of glucobay (acarbose) is 0.5mg/ Mus/sky.Behind the feed 28 days, overnight fasting, caudal vein blood is on an empty stomach gathered in docking, and feeding then behind feeding 2 hours, is plucked eyeball and is got blood.Treat centrifugalize serum behind the blood coagulation, with glucose assays kit measurement blood sugar concentration.
The result: the health care liquid of the inventive method preparation has good blood sugar decreasing effect (seeing the following form) in type-II diabetes animal model Mus.
| Group | Number of animals | Fasting glucose concentration (mg/dL) | Post-prandial glycemia concentration (mg/dL) |
| Experimental group | 9 | 127.97±9.96 | 154.79±32.26 |
| The medicine matched group | 9 | 113.77±12,56 | 133.72±19.57 |
| The normal saline matched group | 9 | 151.86±10.52 | 262.83±25.26 |
Two, to the blood sugar decreasing effect of slight type-II diabetes animal model Mus
Materials and methods: agents useful for same, experimental technique are with the blood sugar decreasing effect experiment to type-II diabetes animal model Mus.Laboratory animal: blood sugar concentration does not meet the requirements of kunming mouse behind the injection alloxan for to the experiment of the blood sugar decreasing effect of type-II diabetes animal model Mus the time, with the conventional mouse forage feed.Animal is divided into 2 groups, promptly keep healthy liquid experimental group and normal saline matched group, two groups of blood glucose values before feed experiment beginning are respectively 168.98 ± 29.23 and 163.51 ± 31.36.By above-mentioned blood sugar decreasing effect experimental technique feed health care liquid and normal saline to type-II diabetes animal model Mus, feed is 40 days altogether.
The result: two treated animals feed after 40 days on an empty stomach the blood sugar concentration of 6 hours (being fasting 6 hours) and empty stomach 16 hours (being fasting 16 hours) see the following form.This result shows that the health care liquid of the inventive method preparation can significantly reduce the blood sugar concentration of slight type-II diabetes animal model Mus (6 hours on an empty stomach), prevent that the blood sugar concentration that long-time hunger (16 hours) is caused from reducing, and shows that this health care liquid has the blood sugar regulation effect.
Three, to the sugared high-protein high-fat food of height to the preventive effect of hyperglycemia
Material: laboratory animal, male mouse of kunming (20g ± 2g), available from Medical Center of Fudan University.Health care liquid, by the inventive method description preparation, its aminoacid (polypeptide) content is 60mg/mL, is 85.61% to the suppression ratio of glycosidase enzyme activity, uric acid content is 29mg/L.High sugared high-protein high-fat feedstuff is with testing with the blood sugar decreasing effect to type-II diabetes animal model Mus.
Method: laboratory animal is raised with high sugared high-protein high-fat feedstuff.Animal is divided into two groups, and by above-mentioned blood sugar decreasing effect experimental technique feed health care liquid and normal saline to type-II diabetes animal model Mus, feed is 40 days altogether.
The result: two treated animals feed after 40 days on an empty stomach the blood sugar concentration of 6 hours (being fasting 6 hours) and empty stomach 16 hours (being fasting 16 hours) see the following form.This result shows that the health care liquid of the inventive method preparation has good preventive effect to the sugared high-protein high-fat food of height institute to the living height of blood glucose.
The health product that the inventive method makes have following characteristics:
1, effectiveness, the health product that prepare with this method had both kept the abundant nutrition composition of silkworm, contained effective blood sugar reducing component again, had blood sugar lowering, nutrition is provided, prevented long-time effects such as hypoglycemia on an empty stomach;
The macromolecular substances and the major part in the silkworm (80%) urate (urate is the main cause that causes gout) that may cause allergic reaction have effectively been removed in 2 safeties, and it is safer that it is taken;
3, convenience has been removed the non-active substance in the silkworm, takes more convenient;
4, quality controllable, by to aminoacid (polypeptide) assay, the glycosidase vigor is suppressed effect measuring and uric acid content measure and carry out quality testing.
The specific embodiment:
Embodiment one:
1, preparation example 1
The freezing preservation silkworm of 52 grams adds 150mL water, with organizing pulverizer to pulverize, boils 5 minutes, and pH to 8.0 is transferred with NaOH in the cooling back, and by adding trypsin, mixing digested 3 hours, boils 2 minutes enzyme denaturing and lives.PH to 4.0-5.0 is transferred with 10NHCl in the cooling back, adds pepsin, digests 3 hours, boils 2 minutes enzyme denaturing and lives.With 2 layers of filtered through gauze, and extract, collect water, the reuse qualitative filter paper filters, and removes all granules.Transfer pH to 7.0, ultrafiltration.The 150mL product, its aminoacid (polypeptide) content is 57mg/mL, is 84.81% to the suppression ratio of glycosidase enzyme activity, uric acid content is 27mg/L.
Get 50 μ L samples in correlation step, 1 times of dilute with water carries out the inhibition experiment of glycosidase, and the result shows that this method has fully kept the effective ingredient (seeing the following form) that can suppress glycosidase in the silkworm.
| Sample | Suppression ratio (%) |
| Silkworm is pulverized the back sample | 92.12 |
| Sample behind the trypsinization | 91.51 |
| Sample behind the pepsin digestion | 84.81 |
| Sample after the ultrafiltration | 86.65 |
Get the lyophilizing of 50mL health care liquid, dry matter weight is 2.4g, and both the 50g silkworm can obtain 7.2g lyophilizing health product.
With the lyophilizing health product of the inventive method preparation send Chinese Academy of Sciences Shanghai biochemical with the used acid cleavage of RESEARCH ON CELL-BIOLOGY after measure amino acid content with automatic amino acid analyzer, amino acid content is 36.64% in these health product as a result, and various amino acid whose compositions see the following form:
| Aminoacid | Millimole | ％ | Aminoacid | Millimole | ％ |
| Taurine TAU | Different bright Ile | 1.73 | |||
| Radix Asparagi Asp | 4.33 | Bright Leu | 2.60 | ||
| The Thr of Soviet Union | 1.59 | Cheese Tyr | 1.56 | ||
| Silk Ser | 1.49 | Phenylpropyl alcohol Phe | 1.93 | ||
| Paddy Glu | 5.41 | Bird ORN | 0.30 | ||
| Dried meat Pro | 0.70 | Rely Lys | 2.61 | ||
| Sweet Gly | 2.59 | Ammonia NH 3 | |||
| Third Ala | 2.32 | Group His | 1.54 | ||
| Guang Cys | 0.41 | Color Try | |||
| Figured silk fabrics Val | 2.36 | Smart Arg | 2.45 | ||
| First sulfur Met | 0.72 |
2, preparation example 2
Freezing the preservations silkworm of 156 grams adds 150mL water, digest as stated above, after the ultrafiltration thin up to 330mL, its, its aminoacid (polypeptide) content is 54mg/mL, is 85.61% to the suppression ratio of glycosidase enzyme activity, uric acid content is 29mg/L.
Embodiment two:
The uric acid reduction is observed in the health product
Because silkworm does not have the organ of urinating, uric acid content is higher in the silkworm, and urate is the main cause that causes gout, thereby we measure the reduction that the inventive method prepares the uric acid of sample: get 2 silkworms (6.3g), add 15mL 0.05M NaOH, the homogenizer cracking, 80 ℃ of water bath heat preservations 1 hour, 10000 rev/mins centrifugal 20 minutes, get supernatant, be diluted to the health care liquid long-pending (20mL) that is equivalent to the preparation of 6.3g silkworm with 0.05M NaOH, measure this sample and the uric acid content of health care in the liquid with testing uric acid test kit (available from Shanghai Rongsheng Bioisystech Co., Ltd), uric acid content is 139.42mg/L in this sample as a result, and the uric acid content in the inventive method preparation health care liquid is 27.20mg/L, explanation can reduce by 80.5% uric acid with the inventive method preparation health care liquid, makes taking of this health care liquid safer.
Claims (4)
1. silkworm health-care product with blood sugar regulation function is characterized in that this silkworm health-care product makes by following method:
(1) processing of silkworm:
3 days 5 ages, silkworm to 7 day silkworm was packed the live body silkworm of raising in the plastic bag into, and 20 ℃ of quick freezing are preserved;
(2) protease digestion:
The silkworm of freezing preservation is taken out, weigh, by weight for the ratio of 0.5-2 adds the deionization purified water, with organizing pulverizer to pulverize, the 0.1-2% heavy by silkworm adds protease digestion, boils enzyme denaturing and lives, cooling, pH2-5 is with 2 layers of filtered through gauze under acid condition, and extracts, and collects water;
(3) ultrafiltration
Supernatant after the above-mentioned filtration is transferred pH to 6-7 with NaOH, filter with tangential flow ultrafilter by peristaltic pump, remove indigested protein, fatty macromolecular substances, filtered solution is standby;
(4) drying:
Above-mentioned steps (3) filtered solution obtained by freeze drying powder is standby;
(5) preparation:
The powder of step (3) filtered solution or step (4) is made the silkworm health-care product preparation according to a conventional method.
2, a kind of preparation method with silkworm health-care product of blood sugar regulation function as claimed in claim 1 is characterized in that this method comprises the following steps:
(1) processing of silkworm:
3 days 5 ages, silkworm to 7 day silkworm was packed the live body silkworm of raising in the plastic bag into, and 20 ℃ of quick freezing are preserved;
(2) protease digestion:
The silkworm of freezing preservation is taken out, weigh, by weight for the ratio of 0.5-2 adds the deionization purified water, with organizing pulverizer to pulverize, the 0.1-2% heavy by silkworm adds protease digestion, boils enzyme denaturing and lives, cooling, pH2-5 is with 2 layers of filtered through gauze under acid condition, and extracts, and collects water;
(3) ultrafiltration
Supernatant after the above-mentioned filtration is transferred pH to 6-7 with NaOH, filter with tangential flow ultrafilter by peristaltic pump, remove indigested protein, fatty macromolecular substances, filtered solution is standby;
(4) drying:
The filtered solution obtained by freeze drying powder art of above-mentioned steps (3) is standby;
(5) preparation:
The filtered solution of above-mentioned steps (3) or the powder of step (4) are made the silkworm health-care product preparation according to a conventional method.
3, the silkworm health-care product preparation method with blood sugar regulation function according to claim 2 is characterized in that the protease in the wherein said step (2) is pepsin, trypsin, neutral protease, animal proteinum hydrolysis compound enzyme, cathepsin, papain or subtilisin.
4, the silkworm health-care product preparation method with blood sugar regulation function according to claim 2 is characterized in that the silkworm health-care product preparation that wherein said step (5) makes is oral liquid, capsule, tablet or granule.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100247014A CN100548315C (en) | 2006-03-15 | 2006-03-15 | Silkworm health-care product and preparation method thereof |
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| CN101036671A CN101036671A (en) | 2007-09-19 |
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| CN110754660A (en) * | 2019-11-22 | 2020-02-07 | 山东寡糖谷生物科技有限公司 | Compound silkworm powder oligosaccharide meal replacement powder capable of reducing blood sugar and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1127637A (en) * | 1995-01-23 | 1996-07-31 | 农村振兴厅 | Agent for reducing blood suger containing powder of silkworm as effective ingredient and making of same |
| CN1258538A (en) * | 1999-12-23 | 2000-07-05 | 蔡双立 | Health food made of silkworm for treating diabetes and hyperlipemia and its production process |
-
2006
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1127637A (en) * | 1995-01-23 | 1996-07-31 | 农村振兴厅 | Agent for reducing blood suger containing powder of silkworm as effective ingredient and making of same |
| CN1258538A (en) * | 1999-12-23 | 2000-07-05 | 蔡双立 | Health food made of silkworm for treating diabetes and hyperlipemia and its production process |
Non-Patent Citations (1)
| Title |
|---|
| 全蚕粉复合物降血糖及降血脂效果的比较. 肖辉等.蚕业科学，第31卷第2期. 2005 * |
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