CN1263695A - Artificially synthetic apomictic allotriploid and monomeric addition line of beet - Google Patents
Artificially synthetic apomictic allotriploid and monomeric addition line of beet Download PDFInfo
- Publication number
- CN1263695A CN1263695A CN99112692A CN99112692A CN1263695A CN 1263695 A CN1263695 A CN 1263695A CN 99112692 A CN99112692 A CN 99112692A CN 99112692 A CN99112692 A CN 99112692A CN 1263695 A CN1263695 A CN 1263695A
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- CN
- China
- Prior art keywords
- beet
- allotriploid
- apomixis
- addition line
- apomictic
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 241000335053 Beta vulgaris Species 0.000 title claims abstract description 31
- 235000016068 Berberis vulgaris Nutrition 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 4
- 241000792395 Patellifolia procumbens Species 0.000 claims description 11
- 238000009396 hybridization Methods 0.000 claims description 9
- 108010044467 Isoenzymes Proteins 0.000 abstract description 2
- 240000007124 Brassica oleracea Species 0.000 abstract 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 abstract 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 abstract 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 abstract 1
- 230000000394 mitotic effect Effects 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
- 210000000349 chromosome Anatomy 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 4
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 4
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 4
- 230000002759 chromosomal effect Effects 0.000 description 3
- 208000020584 Polyploidy Diseases 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 235000021533 Beta vulgaris Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000031864 metaphase Effects 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
An artificially synthesizing method for cultivating apomictic allotriploid beet (VVC) and monomeric addition line beet (VV+1C) includes such steps as cultivating beet, hybridizing beet with cabbage and backcross. After the morphologial and cytologial observation to mitotic interchromosome caryotype, isoenzyme analysis and intergeneration transfer rate analysis, it is proved that the apomitic generation transfer rate is up to 80% for VVC and near 100% for the VV+1C.
Description
The present invention relates to a kind of new germ plasm breeding method, i.e. synthetic apomixis allotriploid beet (VVC) and apomixis monosomic addition line beet (VV+1C) of new synthetic.
Apomixis is meant without spermiovum merges, by the special reproduction phenomenon (clone naturally) of kind of breeding.Apomixis extensively is present in vegetative kingdom, but probability is extremely low to be difficult to utilize because of it occurs, and becomes biological world-famous puzzle.Report is generally limited to observe the apomixis phenomenon both at home and abroad, does not find as yet to solve apomictic Basic Ways.
The objective of the invention is to propose the apomictic Basic Ways of a kind of synthetic.Apomixis allotriploid beet and monosomic addition line beet are cultivated in artificial hybridization.Exist the apomixis phenomenon spending in vain in the beet group polyploid wild species of beet, for the syngamy gene is introduced cultivated beet by cultivation and the distant hybridization of Chinese cabbage beet, synthetic is provided the allotriploid beet (VVC) of apomixis characteristic and is had the chromosomal cultivated beet monosomic addition line of single Chinese cabbage beet (VV+1C).
To the effect that of the present invention: by cultivated beet (Beta vulgaris L) chromosome set VV 2n=18 and Chinese cabbage beet (Beta corolifeora zoss) chromosome set CCCC 2n=36 (V or C represent 9 chromosomes), distant hybridization, cross method is as follows:
1., apomixis allotriploid
Transport is more than 80%
2., apomixis monosomic addition line VV+1C 2n=19 (18+1), have 9 types, one of them be the 100% apomixis monosomic addition line that transmits promptly: VV+1C → VV+1C.Be referred to as the apomixis monosomic addition line.
Allotriploid beet (VVC) has the apomixis characteristic.
Morphological feature shows as open and flat bottle green blade.Plant is in great numbers, obviously tends to wild parent, and root shape is irregular, branches off root more.
Through morphology, the observation of cytology karyotype mitosis metaphase, Isozyme Analysis and transport analytical proof between the generation, apomictic allotriploid beet (VVC) transport that obtains reaches more than 80%, what obtain has a single chromosomal cultivated beet monosomic addition line of beet of spending in vain, and the apomixis transport is bordering on 100%.
Technique effect of the present invention is, by cultivated beet with spend the beet interspecific cross in vain and backcross and obtained transport and reach 80% apomictic allotriploid beet (VVC), the apomixis monosomic addition line that has obtained transport 100% illustrates that we are through distant hybridization, the offspring has selected the successful synthetic of the chromosome engineering new special material that does not have in the world, successful the apomixis gene location of Chinese cabbage beet on individual chromosome, the gene of wild species is guided among the cultivated beet, provided essential condition for cloning first apomixis gene in the world.
In case the apomixis gene clone is come out, just it can be transformed in the cultivated beet, so just can make polyploid hybridization beet, or other hybridization beet and other possible hybrid crop directly carry out the apomixis transmission, as long as such seed is given after the peasant, just do not needed the production of hybrid seeds again, because the hybrid vigour of crossbreed is fixing, as long as peasant oneself results seed just can be as the seed of next year production, they are constant, so will cause the seed institution reforms.
Realize embodiments of the invention, carry out cross experiment in Heilongjiang University bioengineering dept experiment garden:
Embodiment 1: cultivated beet with spend beet hybridization in vain, obtained allotriploid beet (VVC), the apomixis transport reaches 80%.
Embodiment 2: backcrossed again with spending beet hybridization in vain by cultivated beet, obtained totally 9 types of complete added monomer series, spend the specific chromosomal cultivated beet monosomic addition line of beet wherein a kind of having in vain, and the apomixis transport reaches 100%.
Claims (1)
1, synthetic apomixis allotriploid and monosomic addition line beet is characterized in that cross method is by cultivated beet and spend the beet distant hybridization in vain:
1., apomixis allotriploid
Transport is more than 80%
2., apomixis monosomic addition line VV+1C 2n=19 (18+1), have 9 types, one of them be the 100% apomixis monosomic addition line that transmits promptly: VV+1C → VV+1C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99112692A CN1263695A (en) | 1999-02-05 | 1999-02-05 | Artificially synthetic apomictic allotriploid and monomeric addition line of beet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99112692A CN1263695A (en) | 1999-02-05 | 1999-02-05 | Artificially synthetic apomictic allotriploid and monomeric addition line of beet |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1263695A true CN1263695A (en) | 2000-08-23 |
Family
ID=5275974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99112692A Pending CN1263695A (en) | 1999-02-05 | 1999-02-05 | Artificially synthetic apomictic allotriploid and monomeric addition line of beet |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1263695A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103460038A (en) * | 2011-02-23 | 2013-12-18 | 里兰斯坦福初级大学理事会 | Methods of detecting aneuploidy in human embryos |
-
1999
- 1999-02-05 CN CN99112692A patent/CN1263695A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103460038A (en) * | 2011-02-23 | 2013-12-18 | 里兰斯坦福初级大学理事会 | Methods of detecting aneuploidy in human embryos |
| US9879307B2 (en) | 2011-02-23 | 2018-01-30 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of detecting aneuploidy in human embryos |
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| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |