CN1260248C - Active lactoprotein extracted from yak milk and its extraction method and use - Google Patents
Active lactoprotein extracted from yak milk and its extraction method and use Download PDFInfo
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- CN1260248C CN1260248C CNB2004100405964A CN200410040596A CN1260248C CN 1260248 C CN1260248 C CN 1260248C CN B2004100405964 A CNB2004100405964 A CN B2004100405964A CN 200410040596 A CN200410040596 A CN 200410040596A CN 1260248 C CN1260248 C CN 1260248C
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Abstract
The present invention discloses activity milk proteins extracted from yak milk, a method thereof and an application thereof. The activity milk proteins comprise the active components of the following proportion: 51 to 54 of beta-lactoglobulin, 20 to 23 of alpha-lactalbumin, 9.0 to 11 of bovine serum albumin, 1.0 to 2.0 of lactoferrin, 11 to 14 of immune globulin and 1.0 to 2.0 of other micronutrient element. The method comprises the processing steps: selecting raw materials, removing impurities and degreasing by centrifugation, sterilizing by a Pasteur low temperature, coagulating milk, removing bacteria and concentrating by centrifugal filtration, ultra-filtrating, purifying proteins by ion exchanges, filtering in an aseptic mode, evaporating by films, drying in a freezing mode and packaging in aseptic conditions and vacuum. The extracted activity milk proteins processed at the low temperature by a biological technique not only have the obvious functions of enhancing cell activities and organism defence, but also have the positive functions of postponing cell senescence and helping to prevent and cure various diseases; therefore, the present invention can be widely applied to multifunctional food therapeutic products.
Description
Technical field:
The present invention relates to a kind of active milk-protein and biological extraction process and application, particularly a kind of active milk-protein and methods and applications thereof that from yak milk, extract.
Background technology:
Yak is the distinctive domestic animal in high altitude localities, it is unique original poultry kind that does not have breed structure in the Bos domestic animal, galactopoiesis is the important production performance of yak, and yak milk is pastoral area people's important foodstuffs, and milk and dairy products thereof, butter and milk slag are the plateau people's animal food and source of nutrition.Yak has milk fat content and protein content height; be rich in the advantage of nutritive ingredients such as amino acid, multivitamin, trace element, lactose; because yak is raised by feeding and grazing on the All Pure Nature grassland; do not add any artificial diet or additive; so the not only nutrition but also pollution-free of its milk and milk preparation is the high quality raw material of producing the active milk-protein of yak.
Yak can live well on the grassland more than 5500 meters, and other domestic animal is difficult to adapt to.That this grassland has is highly suitable, height above sea level, air pressure is low, oxygen content is few, temperature difference per day is big, the herbage growth phase lacks characteristics, belong to the alpine steppe ecotope that other ox kind is difficult to survive, its inherent factors such as performance and milk-quality characteristic and high and cold grassland ecology environment, feeding and management, herbage nutritional status of giving milk are closely related.The annual production of good monoid yak breast does not wait at 147.00kg-485.00kg, so the raw materials for production of the active milk-protein of yak are very abundant.
Contain beta-lactoglobulin in the yak whey, components such as ALA, lactoferrin, immunoglobulin (Ig) and bovine serum albumin, every kind of albumen composition all has the unique biological effect.But these natural milk-proteins are heat labile, and general milk-product all will pass through high-temperature disinfection treatment in production technique, so just destroyed proteinic structure, make protein lose biological activity.Through consistent proof of animal and human's body research: only activated milk-protein just has diseases prevention and disease-resistant characteristic, and denaturated protein is only had a nutritive value.Up to the present, active milk-protein and the method thereof of utilizing yak milk to extract yet there are no report.
Summary of the invention:
The present invention is intended to overcome above-mentioned defective, and a kind of active milk-protein and methods and applications thereof that extract from yak milk are provided.This method has made full use of the yak peculiar rich nutrient contents of suckling, machining at low temperature through biotechnology, refinement concentrates out composite reactive albumen, and every kind of protein ingredient has not only kept its original biological action, and content is also than the active milk-protein height that extracts from ordinary fresh milk, thereby it has remarkable enhancing cell viability and body defense function, and delaying cell aging and the multiple disease of auxiliary prophylactic treatment are had positive effect.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of active milk-protein that from yak milk, extracts, contained active ingredient and weight proportion are as follows:
Beta-lactoglobulin 51-54
ALA 20-23
Bovine serum albumin 9.0-11
Lactoferrin 1.0-2.0
Immunoglobulin (Ig) 11-14
Other micronutrient element 1.0-2.0
Described other micronutrient element comprises newborn peroxidase, lysosome, multiple amino acids and VITAMIN etc.
A kind of method of the active milk-protein that from yak milk, extracts, its processing step comprises: the choosing of raw material, centrifugal removal of impurities degrease, pasteur cold sterilization, curdled milk, centrifuging degerming concentrate, ultrafiltration, ion-exchange purification albumen, sterile filtration, thin film evaporation, lyophilize and vacuum packaging, and concrete operation is as follows:
Choosing of A, raw material
Choose pure bright natural and pollution-free green yak milk emulsion, the content of its internal protein is 4.5～6.5%.
B, centrifugal removal of impurities degrease
Pure bright natural and pollution-free green yak milk emulsion is positioned over centrifugal stirring in the tophan pot, under 8～10 ℃ of temperature, remove earlier cell and impurity in the yak milk emulsion, slough the fat in the yak milk emulsion again under being lower than 40 ℃ temperature, the removal of impurities degreasing time was controlled at respectively 10 minutes and 20 minutes.
C, pasteur cold sterilization
Adopting the pasteur low-temperature sterilization, the milk of the yak after removal of impurities degreasing emulsion is carried out cold sterilization, promptly is the gamma source irradiation yak emulsion of 5-15KGY with dosage.
D, curdled milk
To place in the storage tank through the milk of the yak behind pasteur cold sterilization emulsion, interpolation rennin or acid curd carry out curdled milk under 35-37 ℃ of temperature, remove casein, promptly get byproduct cheese.
E, centrifuging degerming concentrate
After yak after curdled milk milk emulsion carried out sterile filtration and remove trickle casein particle and bacterium, under 30-35 ℃ of low temperature, concentrate again, obtain the yak whey of crude protein.
F, ultrafiltration
Is that 10000 polymeric membrane carry out the first time filter with molecular weight with the yak whey of crude protein under 30-35 ℃ of low temperature, remove lactose and soluble substance in the yak whey, under 30-35 ℃ of low temperature, be that 15000 polymeric membrane is carried out the second time and filtered again with molecular weight, concentrated milk protein liquid.
G, ion-exchange purification albumen
With pH value is that 5.4 milk-protein liquid carries out ion-exchange purification with electronegative resin anion(R.A), all positively charged protein combine with resin anion(R.A), uncharged fat and lactic acid then are rinsed by resin storage tank, adjust pH value to 7.6, this moment, the protein with resin-bonded was released, and obtained yak milk-protein liquid raw product.
H, sterile filtration
Yak milk-protein liquid behind the purifying is carried out the sterile filtration degerming.
I, thin film evaporation
Be lower than under 40 ℃ of temperature with divided thin film from and remove purifying after milk-protein liquid in moisture, obtain purity and be the active milk-protein more than 96%.
J, lyophilize
After above-mentioned active milk-protein carried out lyophilize, can obtain the active milk-protein finished product of milky powdery yak.
K, asepsis vacuum packing
A kind of active milk-protein that extracts from yak milk is as the application of multifunctional dietotherapeutic healthcare products.
The active milk-protein finished product of described yak can be made into oral granule, chewing lozenge and soft capsule.Its daily dosage is: the adult takes the 10-20 gram every day, and children take the 1-5 gram every day, and the crowd who suffers from various diseases and be in sub-health state is dosage suitably.
The suitable stored refrigerated of finished product of the present invention, the stored refrigerated temperature is 2-8 ℃, the quality guaranteed period is 2-3.
The invention has the advantages that:
1, adopts low temperature pasteurization (gamma method), its sterilisation effect is equal to high temperature instantaneous sterilization method, different is that common milk-product are processed the structure that the high-temperature sterilization method that adopts has been destroyed milk-protein, protein is lost activity, and the low temperature pasteurization can keep the natural radioactivity state of milk-protein effectively, makes its biologically active nourishing function.
2, adopt the low temperature microfiltration method, can further extract and concentrate the yak milk-protein, remove lactose and soluble substance by ultrafiltration and secondary filtration method; A kind of special film is then adopted in the filtration of fat, can pass through protein, and Oil globule is stayed on the film.Using molecular weight is about 10000 polymeric film ultrafiltration protein again, and molecular weight is remained more than or equal to 15000 albumen.By micro-filtration repeatedly yak milk-protein height is concentrated, about 5000 milliliters of pure bright yak breasts can be produced 50 grams and concentrate the yak activated protein, and this product does not need to add any auxiliary material.
3, adopt freeze-drying, can keep yak milk-protein biological activity effectively for a long time, can make the quality guaranteed period of the active milk-protein product of yak reach 2-3.
4, active yak milk-protein finished product have broad-spectrum antimicrobial, antiviral, anti-oxidant, anticancer, regulate organism immune response, regulate gi tract to the absorption of iron and with the association of medicine with effect.
Description of drawings:
Fig. 1 is a process flow sheet of the present invention
Embodiment:
Embodiment 1:
A kind of active milk-protein that from yak milk, extracts, contained active ingredient and weight proportion are as follows:
Beta-lactoglobulin 52.0 grams
ALA 21.5 grams
Bovine serum albumin 11.0 grams
Lactoferrin 1.5 grams
Immunoglobulin (Ig) 12.0 grams
Other micronutrient element 2.0 grams
A kind of method of the active milk-protein that extracts from yak milk, its processing step comprises
Choosing of A, raw material
Choose pure bright natural and pollution-free green yak milk emulsion, the content of its internal protein is 4.5～6.5%.
B, centrifugal removal of impurities degrease
Pure bright natural and pollution-free green yak milk emulsion is positioned over centrifugal stirring in the tophan pot, under 8 ℃ of temperature, remove earlier cell and impurity in the yak milk emulsion, slough the fat in the yak milk emulsion again under 25 ℃ temperature, the removal of impurities degreasing time was controlled at respectively 10 minutes and 20 minutes.
C, pasteur cold sterilization
With the milk of the yak after removal of impurities degreasing emulsion, be the gamma source irradiation yak emulsion of 10KGY with dosage.
D, curdled milk
To place in the storage tank through the milk of the yak behind pasteur cold sterilization emulsion, interpolation rennin or acid curd carry out curdled milk under 37 ℃ of temperature, remove casein, promptly get byproduct cheese.
E, centrifuging degerming concentrate
After yak after curdled milk milk emulsion carried out sterile filtration and remove trickle casein particle and bacterium, under 30 ℃ of low temperature, concentrate again, obtain the yak whey of crude protein.
F, ultrafiltration
Is that 10000 polymeric membrane carry out the first time filter with molecular weight with the yak whey of crude protein under 35 ℃ of low temperature, remove lactose and soluble substance in the yak whey, under 35 ℃ of low temperature, be that 15000 polymeric membrane is carried out the second time and filtered again with molecular weight, concentrated milk protein liquid.
G, ion-exchange purification albumen
With pH value is that 5.4 milk-protein liquid carries out ion-exchange purification with electronegative resin anion(R.A), all positively charged protein combine with resin anion(R.A), uncharged fat and lactic acid then are rinsed by resin storage tank, adjust pH value to 7.6, this moment, the protein with resin-bonded was released, and obtained yak milk-protein liquid raw product.
H, sterile filtration
Yak milk-protein liquid behind the purifying is carried out the sterile filtration degerming.
I, thin film evaporation
Under 20 ℃ of temperature with divided thin film from and remove purifying after milk-protein liquid in moisture, obtain purity and be 98% active milk-protein.
J, lyophilize
After above-mentioned active milk-protein carried out lyophilize, can obtain the active milk-protein finished product of milky powdery yak.
K, asepsis vacuum packing
Embodiment 2:
A kind of active milk-protein that from yak milk, extracts, contained active ingredient and weight proportion are as follows:
See Table 1;
Table 1 yak breast and milk cow's milk main component content contrast (%)
10 yaks breast sample shows that with 10 cow heads breast sample content of mineral substances detected result yak breast phosphorus, iron, magnesium, sodium, calcium, selenium content are apparently higher than the cow's milk of suckling, and potassium is lower than milk cow's milk, significant difference, and P＜0.05 sees Table 2;
Table 2 yak breast and milk cow's milk content of mineral substances contrast (mg/kg)
10 yaks breast sample shows that with 10 cow heads breast sample aminoacids content detected result the yak breast is compared with the cow's milk of suckling, and 17 seed amino acid content are all apparently higher than the cow's milk of suckling, significant difference, P＜0.01; See Table 3
Beta-lactoglobulin 53.1 grams
ALA 21.8 grams
Bovine serum albumin 10.1 grams
Lactoferrin 1.5 grams
Immunoglobulin (Ig) 12.2 grams
Other micronutrient element 1.3 grams
The preparation method is the same.
Embodiment 3:
A kind of active milk-protein that from yak milk, extracts, contained active ingredient and weight proportion are as follows:
Beta-lactoglobulin 52.0 grams
ALA 21.0 grams
Bovine serum albumin 10.0 grams
Lactoferrin 2.0 grams
Immunoglobulin (Ig) 11.0 grams
Other micronutrient element 4.0 grams
The preparation method is the same.
Embodiment 4: pure bright yak breast and pure fresh-milk cow's milk nutrient composition content comparative study result
10 yaks breast sample main component average content shows that with 10 cow heads breast sample main component average content detected result yak breast total protein content and lipid content are apparently higher than the cow's milk of suckling, difference highly significant, P＜0.01; Lactose and ash oontent are close, no significant difference,
Table 3 yak breast and milk cow's milk aminoacids content contrast (%)
Embodiment 5: the active milk-protein quality of yak detects
Adopt poly-propionic acid amide gel electrophoresis detection of active protein content.
Adopt radioimmunodiffusion to detect lactoferrin content.
Embodiment 6: the active milk-protein formulation of yak dosage
A, oral granule type, powdery, every bag 10 gram is taken after mixing it with water with cold boiling water, and the adult obeys 2 bags every day.
B, chewing buccal lozenge type, every 5 gram, the adult contains 4 of clothes every day.
C, soft capsule type, every 3 gram, the adult obeys 6 every day.
Claims (7)
1, a kind of active milk-protein that extracts from yak milk is characterized in that contained active ingredient and weight proportion are as follows:
Beta-lactoglobulin 51-54
ALA 20-23
Bovine serum albumin 9.0-11
Lactoferrin 1.0-2.0
Immunoglobulin (Ig) 11-14
Other micronutrient element 1.0-2.0
Described other micronutrient element comprises newborn peroxidase, lysosome, multiple amino acids and VITAMIN.
2, the extracting method of active milk-protein as claimed in claim 1 is characterized in that processing step is as follows: the choosing of raw material, centrifugal removal of impurities degrease, pasteur cold sterilization, curdled milk, centrifuging degerming concentrate, ultrafiltration, ion-exchange purification albumen, sterile filtration, thin film evaporation, lyophilize and asepsis vacuum packing;
Described pasteur cold sterilization is meant: be the gamma source irradiation yak emulsion of 5-15KGY with the milk of the yak after removal of impurities degreasing emulsion dosage;
Described ultrafiltration is meant: is that 10000 polymeric membrane carry out the first time filter with molecular weight with the yak whey of crude protein under 30-35 ℃ of temperature, remove lactose and soluble substance in the yak whey, under 30-35 ℃ of temperature, be that 15000 polymeric membrane is carried out the second time and filtered again with molecular weight, concentrated milk protein liquid;
Described thin film evaporation and lyophilize are meant: under the 20-40 ℃ of temperature with divided thin film from and remove purifying after milk-protein liquid in moisture after, carry out lyophilize again, can obtain the active milk-protein finished product of milky powdery yak.
3, method as claimed in claim 2, it is characterized in that described centrifugal removal of impurities degrease is meant: pure bright natural and pollution-free green yak milk emulsion is positioned over centrifugal stirring in the tophan pot, under 8-10 ℃ of temperature, remove earlier cell and impurity in the yak milk emulsion, slough the fat in the yak milk emulsion again under 25-40 ℃ temperature, the removal of impurities degreasing time was controlled at respectively 10 minutes and 20 minutes.
4, method as claimed in claim 2 is characterized in that described curdled milk is meant will be positioned in the storage tank through the milk of the yak behind pasteur cold sterilization emulsion, adds rennin or acid curd carries out curdled milk under 35-37 ℃ of temperature, removes casein.
5, method as claimed in claim 2, it is characterized in that described centrifuging degerming concentrates is meant: after the milk of the yak after curdled milk emulsion is carried out sterile filtration and removed trickle casein particle and bacterium, under 30-35 ℃ of low temperature, concentrate again, obtain the yak whey of crude protein.
6, method as claimed in claim 2, it is characterized in that described ion-exchange purification albumen is meant: with pH value is that 5.4 milk-protein liquid carries out ion-exchange purification with electronegative resin anion(R.A), all positively charged protein combine with resin anion(R.A), uncharged fat and lactic acid then are rinsed by resin storage tank, adjust pH value to 7.6, this moment, the protein with resin-bonded was released, and obtained yak milk-protein liquid raw product.
7, the application of a kind of active milk-protein that from yak milk, extracts as claimed in claim 1 in preparation multifunctional dietotherapeutic healthcare products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100405964A CN1260248C (en) | 2004-09-03 | 2004-09-03 | Active lactoprotein extracted from yak milk and its extraction method and use |
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| CNB2004100405964A CN1260248C (en) | 2004-09-03 | 2004-09-03 | Active lactoprotein extracted from yak milk and its extraction method and use |
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| CN1629183A CN1629183A (en) | 2005-06-22 |
| CN1260248C true CN1260248C (en) | 2006-06-21 |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2901796A1 (en) * | 2006-05-31 | 2007-12-07 | Lab Francais Du Fractionnement | PROCESS FOR EXTRACTING ONE OR MORE PROTEINS PRESENT IN MILK |
| CN101248816B (en) * | 2008-03-28 | 2010-07-21 | 新希望乳业控股有限公司 | Low lactose pasteurizing sterilizing milk and preparation thereof |
| CN101362792B (en) * | 2008-09-25 | 2011-07-20 | 上海交通大学 | Affinity separation polymer of lactoferrin and affinity purification method of lactoferrin |
| WO2011130799A1 (en) * | 2010-04-23 | 2011-10-27 | Probiotec Limited | Pharmaceutical compositions |
| CN102150741B (en) * | 2011-03-07 | 2013-08-21 | 马忠仁 | Method for preparing lactalbumin hydrolysate by utilizing yak milk |
| CN102551073B (en) * | 2012-02-22 | 2013-11-20 | 浙江康恩贝健康产品有限公司 | Nutritional composition containing alpha-lactoalbumin and lactoferrin and application of nutritional composition |
| CN102898516A (en) * | 2012-10-26 | 2013-01-30 | 浙江大学 | Method for purifying lactoferrin from milk serum by using an expanded bed adsorption technology |
| CN103271091B (en) * | 2013-05-17 | 2015-04-29 | 北京依科曼生物技术有限公司 | Plant bactericide containing milk protein composition |
| CN106509536A (en) * | 2016-11-10 | 2017-03-22 | 临夏州华安生物制品有限责任公司 | Lily whey beverage and preparation method thereof |
| CN109776666A (en) * | 2019-02-15 | 2019-05-21 | 红原牦牛乳业有限责任公司 | The extracting method of active lactoprotein in yak milk |
| CN110981951B (en) * | 2019-12-26 | 2023-03-28 | 吉林大学 | Preparation method for enriching immunoglobulin and lactoferrin in colostrum |
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2004
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