CN1256317A - Vital nucleus dyeing method - Google Patents
Vital nucleus dyeing method Download PDFInfo
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- CN1256317A CN1256317A CN 99117617 CN99117617A CN1256317A CN 1256317 A CN1256317 A CN 1256317A CN 99117617 CN99117617 CN 99117617 CN 99117617 A CN99117617 A CN 99117617A CN 1256317 A CN1256317 A CN 1256317A
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Abstract
The vital nucleolus dyeing method mainly for medical laboratory features that after vital dyeing of cell with brilliant tar blue, no nucleus and its membrane structure is displayed and the nucleus has the same dyeing effect as cytoplasm. The matter (RNA) inside nucleolus is dyed into blue grains in compact grain array or block mass structure with greater compactness in the center. In the area of the cell outside the nucleolus, there are only sparse blue grains. Therefore, the nucleolus structure is clearly recognizable. The dyeing method is mainly used in recognizing the ripeness of cells and identifying malignant tumor cells and the type of acute leukemia.
Description
The invention belongs to vital nucleus dyeing method, be used for the observation that the microscopically entoblast is made in each medical institutions laboratory.
Usually biological cell contains cytoplasm and nucleus, and nucleus contains kernel.Constituting nuclear major ingredient is thymus nucleic acid (DNA), and the major ingredient that constitutes kernel is Yeast Nucleic Acid (RNA).Kernel have or not and the change of quantity and form has great importance to identification of cell character and maturity.
In order to diagnose the illness by observing kernel under opticmicroscope, nineteen twenty-four Fenlgen etc. has invented the DNA staining, and according to the nuclear dna coloration result, negative person belongs to the intensive place of RNA, promptly is judged as kernel.Cook in 1974 etc. have invented the RNA staining, and the red colouration person is the intensive place of RNA, promptly is judged as kernel.Crocker had founded argyrophilic nucleolar organi-zer regions (AgNOR) staining in 1987, judged kernel, was used to differentiate innocent and malignant tumour.The kernel staining that these are direct or indirect, the agents useful for same kind is many, valency is expensive, difficulty is purchased, operate numerous, requirement for experiment condition height such as PH, temperature, time, coloration result is easily failed, and is difficult in clinical popularization and application.Wright and Giemsa staining are usually used in observing the morphological structure of hemocyte nuclear and slurry.But poor to the kernel Color, be difficult to identification.In fact so far also there is not a kind of simple and easy to do painted good method of entoblast that is specifically designed to.
The objective of the invention is to provide a kind of easy vital nucleus dyeing method, it can clearly tell kernel in cell, for the diagnosis of relative disease provides foundation.
The object of the present invention is achieved like this: cell mix with the Brilliantcresyl Blue dyestuff make vital staining after, the structure of showed cell nuclear and film thereof not, the caryoplasm coloration result is similar.Basophilia material (RNA) in the kernel is dyed blue particle, and it is granular or be overlapped into circular crumb structure that its particle is gathered into fine and close point again, the place near more agglomerate center, and blue particle is overlapping must be intensive more.And in the cell beyond the kernel, blue particle is sparse to be dispersed in distribution, or is linked to each other by blue silk.Set off out obviously the kernel of giving prominence to, being easy to differentiate thus.
The present invention only needs cell and the dyed blended 5-10min of Brilliantcresyl Blue can be laminated and carry out oily sem observation.Easy and simple to handle, condition is easy to control, and kernel obviously easily distinguishes to have application value.
The present invention makes dyestuff with Brilliantcresyl Blue, is mixed with 10g/L Brilliantcresyl Blue ethanolic soln, promptly gets Brilliantcresyl Blue 1g and grinds, and is dissolved among the 95% ethanol 100ml, filters standby.Or be mixed with the Brilliantcresyl Blue salt brine solution, and promptly get Brilliantcresyl Blue 1g, be dissolved among the 109mmol Chinese holly rubber acid sodium 20ml, add 8.5g/L NaCl to 100ml, mixing filters standby.Also available new methylene blue N or reddish black B solution-dyed.
The present invention is in operating process, drip earlier 1 of Brilliantcresyl Blue ethanolic soln on slide glass, after the seasoning; get 1 in tested cell and be placed on mixing on the exsiccant dyestuff; place 5-10min (available two slide glasss overlap cell and dyestuff are clipped in central authorities; to prevent drying), laminate then, under oily mirror, observe.Or on fresh tissue slices, dripping Brilliantcresyl Blue salt brine solution dyeing 10-15min, the oily sem observation in back is done in washing.As shown in Figure 1, cell 1 is after kernel vital staining of the present invention, and the structure of showed cell nuclear and nuclear membrane is not examined similarly to pulp look result, sparse, as to be dispersed in distribution blue particle 2 all occurred.Blue particle 2 be a little granular dense arrangement or overlapping, be fused into crumb structure and be kernel 3.
The present invention is through clinical observation, known particularly hemopathic diagnosis of malignant tumour or differential diagnosis had great importance: the maturity that 1. helps recognizing cells.As initiating cell kernel is arranged, middle late children or mature cell do not have kernel.2. help to differentiate the type of acute leukemia.As myeloblast 2-5 less kernel is arranged, former lymphocyte has 1-2 bigger kernel, former unicellular often be 1 bigger kernel, protoplasmic cell has 2-5 kernel, megakaryoblast has 2-3 kernel, pronormoblast has the individual kernel that differs in size of 1-2.3. judge cellularity.Vigorous or the malignant cell of hyperplasia, blue particle engrain in the caryoplasm, thick, increase.
Claims (3)
1, a kind of vital nucleus dyeing method, it is characterized in that cell is with Brilliantcresyl Blue or new Yamamoto Methylene Blue ZF N or azure B vital staining after, the structure of showed cell nuclear and film thereof is not examined similar to pulp look result.Material (RNA) in the kernel is dyed blue particle, and its particle is a little granular fine and close arrangement or is fused into circular crumb structure, the place near more agglomerate center, and blue particle is overlapping must be intensive more.And in the cell beyond the kernel, blue particle is sparse to be dispersed in distribution, or is connected to thin-cord type by blue silk.
2, vital nucleus dyeing method according to claim 1 is characterized in that doing in marrow, peripheral blood, the dropsy of serous cavity sample vital nucleus dyeing in the vital nucleus dyeing and fresh tissue slices.
3, vital nucleus dyeing method according to claim 1 is characterized in that the prescription of Brilliantcresyl Blue dyestuff is: be mixed with 10g/L Brilliantcresyl Blue ethanolic soln, promptly get Brilliantcresyl Blue 1g and grind, be dissolved among the 95% ethanol 100ml, filter standby.Or be mixed with the Brilliantcresyl Blue salt brine solution, and promptly get Brilliantcresyl Blue 1g, be dissolved among the 109mmol Chinese holly rubber acid sodium 20ml, add 8.5g/L NaCl to 100ml, mixing filters standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991176170A CN1150334C (en) | 1999-08-03 | 1999-08-03 | Vital nucleus dyeing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991176170A CN1150334C (en) | 1999-08-03 | 1999-08-03 | Vital nucleus dyeing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1256317A true CN1256317A (en) | 2000-06-14 |
| CN1150334C CN1150334C (en) | 2004-05-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB991176170A Expired - Fee Related CN1150334C (en) | 1999-08-03 | 1999-08-03 | Vital nucleus dyeing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1150334C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105143847A (en) * | 2013-02-05 | 2015-12-09 | 三路影像公司 | Cytological staining compositions and uses thereof |
-
1999
- 1999-08-03 CN CNB991176170A patent/CN1150334C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105143847A (en) * | 2013-02-05 | 2015-12-09 | 三路影像公司 | Cytological staining compositions and uses thereof |
| CN105143847B (en) * | 2013-02-05 | 2019-05-28 | 三路影像公司 | Cell dyeing composition and application thereof |
| US11274997B2 (en) | 2013-02-05 | 2022-03-15 | Tripath Imaging, Inc. | Cytological staining compositions and uses thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1150334C (en) | 2004-05-19 |
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