CN1244334C - Novel use of traditional Chinese medicine rabdosia rubeseens and Isodon longitubus - Google Patents
Novel use of traditional Chinese medicine rabdosia rubeseens and Isodon longitubus Download PDFInfo
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- CN1244334C CN1244334C CN 03112870 CN03112870A CN1244334C CN 1244334 C CN1244334 C CN 1244334C CN 03112870 CN03112870 CN 03112870 CN 03112870 A CN03112870 A CN 03112870A CN 1244334 C CN1244334 C CN 1244334C
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Abstract
The present invention relates to a new medicine purpose of Chinese herbal medicines-common rabdosia and common rabdosia leaves, namely application of the common rabdosia and the common rabdosia leaves to the preparation of medicine for treating autoimmune diseases. In-vivo and out-of-body experiments are made to a small mouse in terms of immunological function influence by using the water decoction liquid or the alcohol extraction liquid of common rabdosia or the common rabdosia leaf, the results of the experiments prove that the water decoction liquid or the alcohol extraction liquid has immunosuppressive action, and therefore, the present invention can be used for clinically treating the autoimmune diseases. Compared with congener western medicines, the present invention has the advantages of long-term taking, no toxic or side effect, prevention of other opportunistic infections and avoidance of complications.
Description
One, technical field
The present invention relates to a kind of new purposes of Chinese herbal medicine, the new medical use of Chinese herbal medicine Isodon amethystoides (Benth.) C.Y.Wuet Hsuen specifically, its application in preparation treatment autoimmune medicine that Here it is.
Two, background technology
Isodon amethystoides (Benth.) C.Y.Wuet Hsuen (Rabdosia amethytoides) belongs to a kind of in the Labiatae Rabdosia plant.According to the record of " Chinese Higher plant " the 9th volume, this platymiscium has kind more than 100 approximately, fecund in the torrid zone, Asia, subtropical zone and African torrid areas.China has 77 kinds, is distributed in southwest and East China more.At be used for the treatment of impairment caused by overstrain, skin infection and venoms among the people more.From the seventies in 20th century, it is found that Herba Rabdosiae glaucocalycis, Herba Rabdosiae Lophanthoidis and the Rabdosia rubescens etc. in this genus have extremely strong antitumor action, and be made into patent medicine such as tablet, electuary and be used for multiple cancer, and obtain significant curative effect.
Rabdosia Study on plants present situation summarized in " Rabdosia plant research progress " literary composition, and with its pharmacologically active conclude have following several: antibiotic, antitumor, antiinflammatory, protect the liver, antioxidation, protection cardiovascular, suppress platelet aggregation etc. (p.91～92 " Liaoning Chinese medicine and clinical " rolled up for the 2nd phase in 2002 the 5th).
Herba Rabdosiae glaucocalycis is simply arranged, the clinical treatment liver cirrhosis in the disclosed Jingan shierkang capsule compound medicine of CN1315195A.
Regulate the effect of immunologic function about Rabdosia class plant, mostly disclosed be enhancing body's immunological function.Point out that as " the largesepal rabdosia leaf total diterpene is to the regulating action of immune function of mice " literary composition total diterpene all has remarkable facilitation (" Chinese crude drug " 1999.22 (7) p348～350) to humoral immunization and the cellular immunization function of normal and immunologic hypofunction mice.The antitumor mechanism and the cellular immune function relevant (" Chinese Chinese medicine science and technology " 2000.7 (2) .p89～89) that improves tumor mice of Rabdosia excisa pointed out in " Rabdosia excisa is to the experimentation of tumor-bearing mice Immune Function " literary composition.
Under normal circumstances, the immune system of human body protection body has the effect of three aspects, promptly resists the defense reaction of adventitious agents invasion, keeps self balance and stable effect, eliminates the supervision effect of mutant cell.In case immunoreaction abnormity all can cause histologic lesion and causes a disease, claim immune disease.
Immune disease has two kinds, and a kind of is that immunologic hypofunction causes, claims immunodeficiency; To be that immunologic function is extraordinary cause another kind, claims autoimmune disease (autoimmune disease, allergy etc.).Divide two kinds of organ specificity and systemic autoimmune diseases again, the former pathological changes is limited to a certain certain organs, and latter's pathological changes jeopardizes multiple organ and tissue, as systemic lupus erythematosus (sle), rheumatoid arthritis, scleroderma, polymyositis etc.(" pathology ", the third edition, Wu Zhongbi chief editor, the People's Health Publisher publishes April nineteen ninety-five).
(Autoimmunity disease AID) is one of the common refractory disease of serious harm human health to autoimmune disease, does not still have the medicine of being satisfied with at present.For a long time, the standard care that modern medicine is implemented is that the hormone nonspecific immunity suppresses therapy, but can only improve symptom, but can't eliminate the cause of disease, and side effect is bigger.
In recent years, the correlational study of relevant natural drug treatment autoimmune disease is the research focus of pharmacy circle.From at present natural drug being treated the relevant pharmaceutical research of autoimmune disease, natural drug has remarkable advantages than the Western medicine of present clinical use, and it mainly is the effect of bringing into play the treatment autoimmune disease by immunosuppressant, immunomodulating, antioxidation, cell death inducing and the mediator that affects the nerves.The immune pharmacological action of natural drug is not the single effect that is independent of other functions, but the tight unified function system that interrelates with nerve, endocrine, immunomodulating network (NIM).Therefore, both at home and abroad seek the prefered method of the medicine of treatment autoimmune disease from occurring in nature as this type of drug research.
Three, summary of the invention
The applicant utilizes Isodon amethystoides (Benth.) C.Y.Wuet Hsuen (herb, dry product or bright product) decocting liquid or alcohol extract to do in the body and experiment in vitro to mice aspect Immune Function.
In the body experimental selection detect 2 of cellular immune function in the body, the animal model of 4-dinitrofluorobenzene (DNFB) induced mice delayed hypersensitivity, observed the effect of Isodon amethystoides (Benth.) C.Y.Wuet Hsuen decocting liquid or alcohol extract, the result shows that decocting liquid or alcohol extract all can significantly suppress 2, mice delayed hypersensitivity due to the 4-dinitrofluorobenzene (DNFB), i.e. pair cell immunity has inhibitory action.Experiment in vitro is an object of study with the BALB/c mouse spleen lymphocyte, with mitogen concanavalin A, Con A (ConA) activated lymphocyte, adopts thiazole salt (MTT) experimental technique, and vitro detection is subjected to the influence of reagent thing to lymphproliferation response.The pastille serum of having observed Isodon amethystoides (Benth.) C.Y.Wuet Hsuen decocting liquid or alcohol extract is induced the influence of spleen lymphocyte propagation to ConA, and the result shows: the pastille serum of decocting liquid or alcohol extract all can suppress lymphopoiesis, has immunosuppressive action.Isodon amethystoides (Benth.) C.Y.Wuet Hsuen decocting liquid or alcohol extract all have immunosuppressive action, and do not find the atrophy of immune organ.
The immunosuppressive action of Isodon amethystoides (Benth.) C.Y.Wuet Hsuen decocting liquid or alcohol extract can be used for the clinical treatment autoimmune disease, compares with similar Western medicine, has no side effect, and the patient can adhere to taking for a long time, and can prevent other opportunistic infection, avoids complication.
Four, the specific embodiment
1, get the bright product (or dry product) of Isodon amethystoides (Benth.) C.Y.Wuet Hsuen herb, remove rotted leaf, clean back airing, chopping, add water or ethanol, decoct or refluxed 1～3 hour, the separation of cooling back promptly getting decocting liquid or alcohol extract.
2, alcohol extract should not be taken clinically, though that decocting liquid can be taken is more loaded down with trivial details, and suitable electuary or capsule.Soon decocting liquid or alcohol extract precipitation concentrate and are extractum, and it is encapsulated that extractum becomes electuary or extract dry to be powder with adjuvant mixing post-treatment.
The 100kg dry product can obtain 15～16kg extractum, can process to obtain 90～100kg electuary, and Coming-of-Age Day obeys three times, each 10～20g.
3, make the experimentation of anti-delayed hypersensitivity during the electuary (the anti-furuncle halberd of trade name electuary) that obtains with Isodon amethystoides (Benth.) C.Y.Wuet Hsuen processing is studied by Shanghai Univ. of Traditional Chinese Medicine's new drug pharmacological toxicology, the result is as follows:
(1) purpose
Observe the effect of the anti-delayed hypersensitivity of anti-furuncle halberd electuary, and compare with the dexamethasone injection.
(2) material
1, medicine:
(1) anti-furuncle halberd electuary, brown particle, 20 gram/bags, the each bag of human, is provided lot number: 950422 at day three times by Su County, Anhui.Time spent is not diluted to desired concn with physiology salt.
(2) dexamethasone injection, colourless transparent liquid, 2 milligrams/1 milliliter, Shanghai the 9th pharmaceutical factory produces, lot number: 940503.Time spent is made into the suspension of desired concn with normal saline.
2, sensitizing agent: 2,4-dinitrofluorobenzene (DNFB), AR level, Wujiang high official position Fine Chemical Works product, lot number: 931215.
3, animal: kunming mouse, male and female half and half, body weight 30 ± 2g is provided by this school Experimental Animal Center, the quality certification number: the moving word 02-49-1 of doctor.This school laboratory (quality certification number: the moving word 02-24 of doctor) raise one day after, enter experiment.
(3) method
With mice body weight, sex equilibrium, be divided into six groups at random: normal control group (normal saline 10ml/Kg/ time, 2 times/day, 1 time/day), model control group (normal saline 10ml/Kg/ time, 2 times/day), anti-furuncle halberd electuary high dose group (4.8g/Kg/ time, 1 time/day), dosage group (2.4g/Kg/ time, 1 time/day) in the anti-furuncle halberd electuary, anti-furuncle halberd electuary low dose group (1.2g/Kg/ time, 1 time/day) and dexamethasone matched group (30ug/Kg/ time, 1 time/day), 10 every group.Medication the previous day, every Mus abdominal part depilation (3 * 3cm).Second day, every Mus abdominal part was smeared 1%DNFB acetone Oleum Sesami (1: 1) solution 50ul sensitization, the administration of dividing into groups immediately, continuous seven days.Administration the 6th day, every Mus is applied in left ear with above-mentioned 1%DNFB solution 10ul and attacks.After 24 hours (last administration one hour), put to death animal, lay left and right auricle with card punch, analytical balance is weighed, with the difference of left and right auricle weight as inflammation swelling index; Take out thymus, spleen, analytical balance is weighed, and obtains its organ index.Data are checked the analysis that takes statistics with T-, relatively the significance of difference between each group.The significance of difference that compares the body weight change (forward and backward difference) of each treated animal simultaneously.
(4) result
See Table
Medicine to the influence of rat delayed hypersensitivity and immune organ index thereof (X ± SD, n=10)
| Group | Normal control group (10ml/Kg/ day) | Model control group (10ml/Kg/ day) | Dexamethasone matched group (30ug/Kg/ day) | The heavy dose of group of anti-furuncle halberd (4.8g/Kg/ day) | Dosage group in the anti-furuncle halberd (2.4g/Kg/ day) | Anti-furuncle halberd small dose group (1.2g/Kg/ day) |
| Swelling degree (mg) body weight change (g) thymus index index and spleen index | -0.70±1.16 5.68±3.31 0.284±0.075 0.592±0.202 | 9.10+2.85 4.79±3.62 0.299±0.100 0.604±0.131 | 2.30±1.77** 2.94±3.83 0.230±0.064 0.375±0.186* | 2.70±1.95** 2.98±3.43 0.268±0.054 0.4.34±0.105* | 2.50±2.17** 2.72±3.03 0.219±0.058* 0.519±0.105 | 7.00±2.88 3.69±2.68 0.306±0.06* 0.565±0.13* |
Annotate: 1. compare * P＜0.05 with model group; * P＜0.01
(5) conclusion
Anti-furuncle halberd electuary has definite inhibitory action to delayed hypersensitivity, and (P＜0.01=is similar to the effect of dexamethasone, and the trend that suppresses growth is also arranged, but statistical test difference not significantly (P＞0.05).
Claims (1)
1, a kind of new medical use of Chinese herbal medicine Isodon amethystoides (Benth.) C.Y.Wuet Hsuen is characterized in that: the application of Isodon amethystoides (Benth.) C.Y.Wuet Hsuen in preparation treatment autoimmune medicine.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03112870 CN1244334C (en) | 2003-02-21 | 2003-02-21 | Novel use of traditional Chinese medicine rabdosia rubeseens and Isodon longitubus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 03112870 CN1244334C (en) | 2003-02-21 | 2003-02-21 | Novel use of traditional Chinese medicine rabdosia rubeseens and Isodon longitubus |
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| CN1522723A CN1522723A (en) | 2004-08-25 |
| CN1244334C true CN1244334C (en) | 2006-03-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1951430B (en) * | 2006-09-29 | 2010-05-12 | 宿州绿源中医药科技有限公司 | Compound Chinese medicine for treating psoriasis and lupus erythematosus |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530164A (en) * | 2009-04-20 | 2009-09-16 | 上海慈翔生物医学研究所（普通合伙） | Feed additive for beasts and birds replacing antibiotics and preparation method thereof |
| CN104171206A (en) * | 2014-08-25 | 2014-12-03 | 宿州绿源中医药科技有限公司 | Preparation method of common rabdosia leaf tea |
| CN104435095B (en) * | 2014-11-14 | 2017-12-26 | 宿州学院 | The total terpene active component of common rabdosia leaf and its extracting method, application, composition |
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2003
- 2003-02-21 CN CN 03112870 patent/CN1244334C/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1951430B (en) * | 2006-09-29 | 2010-05-12 | 宿州绿源中医药科技有限公司 | Compound Chinese medicine for treating psoriasis and lupus erythematosus |
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| Publication number | Publication date |
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| CN1522723A (en) | 2004-08-25 |
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