CN1237636A - Bioconversion method of selenium and zinc by utilizing beneficial bacterium - Google Patents
Bioconversion method of selenium and zinc by utilizing beneficial bacterium Download PDFInfo
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- CN1237636A CN1237636A CN 98101995 CN98101995A CN1237636A CN 1237636 A CN1237636 A CN 1237636A CN 98101995 CN98101995 CN 98101995 CN 98101995 A CN98101995 A CN 98101995A CN 1237636 A CN1237636 A CN 1237636A
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- substratum
- zinc
- selenium
- hours
- transformation
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- 239000011669 selenium Substances 0.000 title claims abstract description 24
- 239000011701 zinc Substances 0.000 title claims abstract description 24
- 229910052725 zinc Inorganic materials 0.000 title claims abstract description 22
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 21
- 241000894006 Bacteria Species 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 18
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 title claims abstract description 17
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 16
- 230000009286 beneficial effect Effects 0.000 title abstract 2
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 229940091258 selenium supplement Drugs 0.000 claims abstract description 15
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 9
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 9
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 9
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 8
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 8
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 8
- 239000011781 sodium selenite Substances 0.000 claims abstract description 8
- 230000001580 bacterial effect Effects 0.000 claims abstract description 7
- 235000018291 probiotics Nutrition 0.000 claims description 16
- 239000006041 probiotic Substances 0.000 claims description 15
- 230000036983 biotransformation Effects 0.000 claims description 13
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 8
- 230000009466 transformation Effects 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 238000009792 diffusion process Methods 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 210000001161 mammalian embryo Anatomy 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 239000011573 trace mineral Substances 0.000 abstract description 19
- 235000013619 trace mineral Nutrition 0.000 abstract description 19
- 239000001963 growth medium Substances 0.000 abstract description 2
- 241000186000 Bifidobacterium Species 0.000 abstract 1
- 241000186660 Lactobacillus Species 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 230000000144 pharmacologic effect Effects 0.000 abstract 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 11
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 240000001929 Lactobacillus brevis Species 0.000 description 1
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000003321 atomic absorption spectrophotometry Methods 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 230000007661 gastrointestinal function Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 description 1
- MCAHWIHFGHIESP-UHFFFAOYSA-N selenous acid Chemical compound O[Se](O)=O MCAHWIHFGHIESP-UHFFFAOYSA-N 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The biological conversion method of inorganic elements selenium and zinc into organic trace elements by utilizing beneficial bacterial strain includes the following steps: the activated bifidobacterium, lactobacillus and LD bacterium each 0.5% according to their volume weight ratio respectively are added into 1000 ml culture medium, then a certain quantity of sodium selenite and zinc sulfate are respectively added, symbiotically-fermented at 38 plus or minus 1, deg.C. and fermentation conversion time is about 24 hr, so that the inorganic trace elements can be converted into bio-state organic trace elements so as to raise the biological utilization of trace element, at the same time the pharmacologic activity of benficial bacterial strain is raised.
Description
The present invention relates to utilize bacterial classification inorganic elements to be carried out the method for organic transformation, specifically be meant a kind of method of utilizing probiotics selenium and zinc to be carried out bio-transformation, make inorganic trace element be converted into the organic trace element of biological attitude by this conversion, improve micro-bioavailability, improved the pharmacologically active of probiotics simultaneously.
Selenium and zinc are indispensable trace elements among the human life activity, they for human body grow and the cancer-resisting aspect has play a part important.Though inorganic trace element can synthesize the required biologically active substance of body, can not improve the level of stocking of the intravital trace element of machine.
As everyone knows, the organic trace element of biological attitude is a human body trace element supplement valid approach.We find through years of researches, and bifidus bacillus, Bacterium lacticum and DL bacterium are the probioticss to human body, and its thalline and fermentating metabolism product can improve the gastrointestinal function of human body, improve the immunologic function of human body.Probiotics is in the growth metabolism process, enter cell by the special absorption trace element of the transfer system of cytoplasmic membrane, make inorganic trace element turn to the organic trace element of biological attitude, improved micro-bioavailability, improved the pharmacologically active of probiotics simultaneously.
Provided by the present inventionly utilize probiotics that selenium and zinc are carried out bacterial classification related in the method for bio-transformation to comprise bifidus bacillus B.breve, Bacterium lacticum L.delbrueckii, LD bacterium E.faecalis.
The method of utilizing probiotics selenium and zinc to be carried out bio-transformation provided by the present invention: with activatory bifidus bacillus, Bacterium lacticum and LD bacterium each by volume the ratio of weight ratio 0.5% join in 1000 milliliters the substratum, add Sodium Selenite and zinc sulfate respectively, at 38 ± 1 ℃ of symbiotic fermentations, the fermentation transformation time is about 24 hours.
Consisting of (parts by weight are represented) of wherein said substratum
Soybean embryo tooth albumen conversion fluid 90-110
Protein enzyme solution 3-7
Glucose 0.5-2.5
The female diffusion juice 0.3-0.9 of acid
Sucrose 1-5
The preferred group of wherein said substratum becomes (parts by weight are represented)
Soybean embryo tooth albumen conversion fluid 100
Protein enzyme solution 5
Glucose 1.5
The female diffusion juice 0.6 of acid
Sucrose 3
The probiotics that utilizes provided by the present invention carries out in the method for bio-transformation selenium and zinc, and the activatory process of related bacterial classification is respectively: bifidus bacillus is in the BS substratum, in 38 ± 1 ℃ of cultivations 18 hours, by the inoculation of 0.5% inoculum size; Bacterium lacticum was cultivated 5 hours for 38 ± 1 ℃ in the LC substratum, by the inoculation of 0.5% inoculum size; The DL bacterium was cultivated 12 hours for 38 ± 1 ℃ in the EC substratum, by the inoculation of 0.5% inoculum size.
The probiotics that utilizes provided by the present invention carries out in the method for bio-transformation selenium and zinc, and the add-on of related Sodium Selenite is the substratum of 0.001-0.01g/1000mL, and the add-on of zinc sulfate is the substratum of 0.01-0.04g/1000mL; At 38 ± 1 ℃ of symbiotic fermentations, the fermentation transformation time is about 24 hours.
The probiotics that utilizes provided by the present invention carries out in the method for bio-transformation selenium and zinc, and the optimal addn of related Sodium Selenite is the substratum of 0.0024g/1000mL, and the add-on of zinc sulfate is the substratum of 0.024g/1000mL; At 38 ± 1 ℃ of symbiotic fermentations, the fermentation transformation time is about 24 hours.
By the method that selenium and zinc are carried out bio-transformation of the present invention, make inorganic trace element can be converted into the organic trace element of biological attitude, can improve the bioavailability of trace element, improved the pharmacologically active of probiotics simultaneously.This can be illustrated by following embodiment.
Embodiment 1
At first with bifidus bacillus in the BS substratum, cultivated 18 hours in 38 ± 1 ℃; Bacterium lacticum was cultivated 5 hours for 38 ± 1 ℃ in the LC substratum; The DL bacterium was cultivated 12 hours for 38 ± 1 ℃ in the EC substratum; Three strain bacterium are in 38 ± 1 ℃ of environment, consisting of (parts by weight are represented) 100 portions of soybean embryo tooth albumen conversion fluid: 5 parts of protein enzyme solutions: 1.5 parts of glucose: 0.6 part of female diffusion juice of acid: symbiotic fermentation in the substratum of 3 portions of sucrose, the amount of the bifidus bacillus that adds in wherein every 1000mL substratum, Bacterium lacticum, DL bacterium is respectively 5g; Do not add the group in contrast of Sodium Selenite and zinc sulfate, 0.0024g selenous acid that adds in the 1000mL substratum and 0.024g zinc sulfate are as experimental group, and the fermentation transformation time is about 24 hours.
The result who transforms through above-mentioned method is:
(1) contains the collection and the pre-treatment of Se and Zn thalline.
A certain amount of nutrient solution 2000vpm of accurate absorption, 10 minutes centrifugal, uses distilled water wash four times, collects thalline, and be settled to the volume of getting with distilled water.
(2) assay of Se, Zn element
Adopt atomic absorption spectrophotometry and atomic fluorescence spectrum.
To not planting in the bacterium culture medium, Se and Zn content are measured result such as following table in bacterial classification experimental group that washing is collected and the control group solution:
| The adding Se and the Zn that do not plant bacterium cultivate blank | The thalline of in adding Se and Zn element substratum, growing | The thalline of in not adding Se and Zn element substratum, growing | |
| ?Se(mg/ml) | ????1.18 | ????0.38 | ????0.032 |
| ?Zn(mg/ml) | ????13.2 | ????0.34 | ????0.22 |
From last table result, in containing the culture environment of Se, the trace element that thalline contains is than not containing high nearly 12 times of the thalline of growing in the environment of selenium; As if in zinciferous culture environment, the thalline of growing in the environment of the trace element that thalline contains than zinc selenium not is also high more than 1 times, and the bio-transformation of zinc is not obvious, this may be higher with the thalline zinc content of blank and thalline to absorb degree of conversion relevant.
Related instrument, equipment comprises incubator, whizzer, P-E5000 type atomic absorption spectrophotometer in the present embodiment, and the U.S. produces multi-functional atomic spectrophotofluorometer, Beijing product etc.
Claims (4)
1, a kind of method of utilizing probiotics selenium and zinc to be carried out bio-transformation, comprising with activatory bifidus bacillus, Bacterium lacticum and LD bacterium each by volume the ratio of weight ratio 0.5% join in 1000 milliliters the substratum, add a certain amount of Sodium Selenite and zinc sulfate respectively, at 38 ± 1 ℃ of symbiotic fermentations, the fermentation transformation time is about 24 hours; Consisting of (parts by weight are represented) of wherein said substratum
Soybean embryo tooth albumen conversion fluid 100;
Protein enzyme solution 5;
Glucose 1.5;
The female diffusion juice 0.6 of acid;
Sucrose 3.
2, the described method of utilizing probiotics that selenium and zinc are carried out bio-transformation of claim 1, the activatory process of wherein related bacterial classification is respectively: bifidus bacillus is in the BS substratum, in 38 ± 1 ℃ of cultivations 18 hours, by the inoculation of 0.5% inoculum size; Bacterium lacticum was cultivated 5 hours for 38 ± 1 ℃ in the LC substratum, by the inoculation of 0.5% inoculum size; The DL bacterium was cultivated 12 hours for 38 ± 1 ℃ in the EC substratum, by the inoculation of 0.5% inoculum size.
3, the described method of utilizing probiotics that selenium and zinc are carried out bio-transformation of claim 1, wherein the add-on of related Sodium Selenite is the substratum of 0.001-0.01g/1000mL, the add-on of zinc sulfate is the substratum of 0.01-0.04g/1000mL; At 38 ± 1 ℃ of symbiotic fermentations, the fermentation transformation time is about 24 hours.
4, the described method of utilizing probiotics that selenium and zinc are carried out bio-transformation of claim 1, wherein the add-on of related Sodium Selenite is the substratum of 0.0024g/1000mL, the add-on of zinc sulfate is the substratum of 0.024g/1000mL; At 38 ± 1 symbiotic fermentations, the fermentation transformation time is about 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98101995A CN1087781C (en) | 1998-05-28 | 1998-05-28 | Bioconversion method of selenium and zinc by utilizing beneficial bacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98101995A CN1087781C (en) | 1998-05-28 | 1998-05-28 | Bioconversion method of selenium and zinc by utilizing beneficial bacterium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1237636A true CN1237636A (en) | 1999-12-08 |
| CN1087781C CN1087781C (en) | 2002-07-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98101995A Expired - Lifetime CN1087781C (en) | 1998-05-28 | 1998-05-28 | Bioconversion method of selenium and zinc by utilizing beneficial bacterium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1087781C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416681B (en) * | 2008-11-25 | 2011-09-07 | 丁岚峰 | Selenium-rich Enterococcus faecium cernelle three-layer microcapsule and preparation method and use thereof |
| WO2018192501A1 (en) * | 2017-04-17 | 2018-10-25 | 江苏德禧生物科技有限公司 | Microbial agent for prevention of intestinal toxicity due to tumor chemotherapy |
| WO2019010813A1 (en) * | 2017-07-13 | 2019-01-17 | 吴炳新 | Microbial fermentation composition and use thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094152C (en) * | 1994-06-21 | 2002-11-13 | 太原康强药业有限公司 | Microbiological medicine containing rich selenium for health-care and therapy and its preparation |
| CN1053471C (en) * | 1994-09-18 | 2000-06-14 | 中山大学 | Production of zinc gluconate by fermenting glucose using Aspergillus niger |
-
1998
- 1998-05-28 CN CN98101995A patent/CN1087781C/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416681B (en) * | 2008-11-25 | 2011-09-07 | 丁岚峰 | Selenium-rich Enterococcus faecium cernelle three-layer microcapsule and preparation method and use thereof |
| WO2018192501A1 (en) * | 2017-04-17 | 2018-10-25 | 江苏德禧生物科技有限公司 | Microbial agent for prevention of intestinal toxicity due to tumor chemotherapy |
| WO2019010813A1 (en) * | 2017-07-13 | 2019-01-17 | 吴炳新 | Microbial fermentation composition and use thereof |
| US11376292B2 (en) * | 2017-07-13 | 2022-07-05 | Bingxin WU | Microbial fermentation composition subjected to enzymolysis, microbial fermentation and microbial transformation and use thereof |
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| Publication number | Publication date |
|---|---|
| CN1087781C (en) | 2002-07-17 |
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Owner name: FENG SHUQIN Free format text: FORMER OWNER: SANZHU PHARMACEUTICAL INDUSTRY CO., LTD., JINAN CITY Effective date: 20130628 Owner name: FENG SHUXIA FENG SHUQING Effective date: 20130628 |
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Free format text: CORRECT: ADDRESS; FROM: 250100 JINAN, SHANDONG PROVINCE TO: 250001 JINAN, SHANDONG PROVINCE |
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| TR01 | Transfer of patent right |
Effective date of registration: 20130628 Address after: 301, room 1, unit 1, building 39-1, 250001 Shandong Road, Lixia Road, Lixia District, Shandong, Ji'nan Patentee after: Feng Shuqin Patentee after: Feng Shuxia Patentee after: Feng Shuqing Address before: 250100 No. 77 North Grand Road, Shandong, Ji'nan Patentee before: Sanzhu Pharmaceutical Industry Co., ltd., Jinan City |
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Granted publication date: 20020717 |
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