CN1236813A - 葡萄球菌快速分离鉴定培养基 - Google Patents
葡萄球菌快速分离鉴定培养基 Download PDFInfo
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Abstract
本发明涉及一种葡萄球菌快速分离鉴定培养基。主要解决现有的用于葡萄球菌分离培养的血平板易生杂菌、检验程序复杂的问题。技术方案:一种葡萄球菌快速分离鉴定培养基,含有的成分及重量百分比为,蛋白胨0.85—1.50、酵母浸膏0.10—0.25、氯化钠7.30—7.60、甘露醇0.70—1.80、明胶2.80—3.20、磷酸氢二钾0.40—0.60、琼脂1.50—2.00、蒸馏水100。本发明不易生长杂菌、检验程序简单。
Description
本发明涉及葡萄球菌快速分离鉴定培养基。
现在我国临床、预防、药检及商检等机构对金黄色葡萄球菌的检验方法为先培菌、再分离培养于血平板，然后做生化反应和血浆凝固酶等繁锁复杂的试验。用血平板培养葡萄球菌容易生长杂菌，发生变形菌弥漫生长而无法观察的现象，并且用血平板做鉴定试验程序复杂，检测不便，尤其不适应基层检验工作的需要。
本发明的目的在于提供一种培养葡萄球菌不易生长杂菌、检验程序简单的葡萄球菌快速分离鉴定培养基。
本发明的技术方案；一种葡萄球菌快速分离鉴定培养基，其含有的成分及重量百分比为，蛋白胨0.85-1.50、酵母浸膏0.10-0.25、氯化钠7.30-7.60、甘露醇0.70-1.80、明胶2.80-3.20、磷酸氢二钾0.40-0.60、琼脂1.50-2.00、蒸馏水100。
本发明的有益效果：用本发明分离培养葡萄球菌与血平板培养葡萄球菌相比，不易生杂菌。做实验，将105株金黄色葡萄球菌分别接种于本发明和血平板，经培养有32株葡萄球菌在血平板上因变形菌弥漫生长而无法观察，而在本发明生长的葡萄球菌状况良好，无任何杂菌生长。对66株葡萄球菌重复性试验结果表明，本发明对葡萄球菌分离鉴定重复性好，本发明于4℃冰箱中保存，6个月后质量稳定，使用效果良好。用本发明从接种到初步鉴定报告葡萄球菌检查结果在43小时内即可完成，有助于临床感染及食物中毒病原菌的快速诊断，应用方便价格低廉，改变了过去分离鉴定需时间长、试剂消耗高、程序繁杂、结果不够准确的弊端。
下面叙述本发明的实施例。
制备本发明时，蛋白胨可选用牛肉胨或多胨。还可加入重量百分比不大于0.2的乳糖。取牛肉胨10克、酵母浸膏2.5克、氯化钠75克、明胶30克、甘露醇10克、磷酸氢二钾5克、乳糖2克、琼脂15克，加蒸馏水1000ml，将前述物质溶于水，溶解后调PH7.0±0.1，分装三角烧瓶中121℃灭菌150分钟，冷却后冰箱保存备用。
当需用本发明检验时，将本发明表面充分干燥，以白金耳划线接种置37℃,36-43小时培养后观察菌落形态、耐盐性色素形成、甘露醇发酵和明胶液化。观察甘露醇发酵时，直接滴0.04％BTB于菌落，立即观察结果菌落及周围呈黄色为阳性；观察明胶液化时，需滴加20％磺胺硫酸水溶液或硫酸饱和溶液，2分钟内，在白浊培养基上的菌落周围呈现明显透明环为阳性。
Claims (3)
1、一种葡萄球菌快速分离鉴定培养基，其含有的成分及重量百分比为，蛋白胨0.85-1.50、酵母浸膏0.10-0.25、氯化钠7.30-7.60、甘露醇0.70-1.80、明胶2.80-3.20、磷酸氢二钾0.40-0.60、琼脂1.50-2.00、蒸馏水100。
2、根据权利要求1所述的葡萄球菌快速分离鉴定培养基，其特征在于：蛋白胨可以为牛肉胨或多胨。
3、根据权利要求1或2所述的葡萄球菌快速分离鉴定培养基，其特征在于：还可加入重量百分比不大于0.2的乳糖。
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| Application Number | Priority Date | Filing Date | Title |
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| CN 98113951 CN1236813A (zh) | 1998-04-27 | 1998-04-27 | 葡萄球菌快速分离鉴定培养基 |
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| CN 98113951 CN1236813A (zh) | 1998-04-27 | 1998-04-27 | 葡萄球菌快速分离鉴定培养基 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100345977C (zh) * | 2006-03-14 | 2007-10-31 | 南京天邦生物科技有限公司 | 一种致奶牛乳房炎葡萄球菌培养液的配制方法及其用途 |
| WO2021044205A1 (en) * | 2019-09-03 | 2021-03-11 | Naveen Kumar Arora | A process for preparing a vegan media for cultivation of bacteria, fungi and plant seedling and composition thereof |
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1998
- 1998-04-27 CN CN 98113951 patent/CN1236813A/zh active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100345977C (zh) * | 2006-03-14 | 2007-10-31 | 南京天邦生物科技有限公司 | 一种致奶牛乳房炎葡萄球菌培养液的配制方法及其用途 |
| WO2021044205A1 (en) * | 2019-09-03 | 2021-03-11 | Naveen Kumar Arora | A process for preparing a vegan media for cultivation of bacteria, fungi and plant seedling and composition thereof |
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