CN1234848C - Bacterium culture medium and preparation method therefor - Google Patents
Bacterium culture medium and preparation method therefor Download PDFInfo
- Publication number
- CN1234848C CN1234848C CN 200410012136 CN200410012136A CN1234848C CN 1234848 C CN1234848 C CN 1234848C CN 200410012136 CN200410012136 CN 200410012136 CN 200410012136 A CN200410012136 A CN 200410012136A CN 1234848 C CN1234848 C CN 1234848C
- Authority
- CN
- China
- Prior art keywords
- water
- culture medium
- nutrient agar
- agent
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a bacterium culture medium and a preparation method thereof, particularly to a bacterium culture medium using a nutrient agar culture medium as a basal culture medium and a preparation method thereof. The nutrient agar medium is used as the basal culture medium; water preserving agents and coloring agents are added in the basal culture medium; the weight ratio of the nutrient agar to water to the water preserving agents to the coloring agents is equal to 36 to 40: 1000: 0.1 to 5: 0.01 to 0.1; the components weight according to the weight ratio are mixed and sterilized in a preparation process. The present invention overcomes the defects of difficult observation, complicated calculation and measured results, and complicated measure processes in the prior art; the present invention has the characteristics of maintenance in the water content of the culture medium, convenient observation of measured results, reasonable preparation method and technological design, and simple operation.
Description
Technical field
The present invention relates to a kind of bacteria culture medium and preparation method thereof, particularly a kind of is the bacteria culture medium and preparation method thereof of basic medium with the nutrient agar.
Background technology
At present, the bacteria culture medium major part is matrix with agar in the prior art, and adds the required nutrition of some bacterial growths, according to the strict aseptic manipulation program bacterium is cultivated, observes and measures in the aseptic experiment chamber then.Exist in the above-mentioned background technology trace routine loaded down with trivial details, can not direct viewing and calculate the shortcoming of measurement result.
Summary of the invention
The object of the present invention is to provide a kind of bacterium colony situation that is easy to observe, avoid the substratum dehydration simultaneously, influence bacteria culture medium of measurement result and preparation method thereof.
General idea of the present invention is:
It is a basic medium with the nutrient agar, also is added with water-holding agent and staining agent in the basic medium, and wherein, nutrient agar medium: water: water-holding agent: the weight ratio of staining agent is 36-40: 1000: 0.1-5: 0.01-0.1.
The preparation method of bacteria culture medium comprises following step:
A, earlier with nutrient agar medium: water: water-holding agent: staining agent is by 36-40: 1000: 0.1-5: after the weight ratio of 0.01-0.1 is weighed, placed apart;
B, load weighted nutrient agar medium and water are mixed with nutrient agar;
Add load weighted water-holding agent in c, the nutrient agar that in above-mentioned steps, prepares;
D, the substratum that contains water-holding agent for preparing in the c step is sterilized;
After the staining agent sterilization of e, weighing, add in the substratum of d step preparation, promptly make bacteria culture medium.
Other designs of the present invention are:
Nutrient agar medium in the bacteria culture medium: water: water-holding agent: the weight ratio of staining agent is 36-40: 1000: 0.1-1: 0.01-0.1.
Water-holding agent selects for use starch graft copolymer vinylformic acid super strength water absorbent, starch graft copolymer acrylate super strength water absorbent, modified-cellulose class High hydrophilous resin or acryloyl derivative and inorganics to be combined into High hydrophilous resin, staining agent select for use the PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine, Congo red, the PHENOL 99.8 MIN ((CARBOLIC ACID)) sargassun is red, Viola crystallina, methyl orchid or TCC.
Preparation is during bacteria culture medium, and with nutrient agar medium: water: water-holding agent: staining agent is pressed 36-40: 1000: 0.1-1: the weight ratio weighing of 0.01-0.1.
Nutrient agar medium is mixed placement 8-12 minute with water, heat then, nutrient agar medium is fully dissolved.
When adding water-holding agent, elder generation adds in the nutrient agar after water-holding agent is ground into 80-100 purpose particle again, stirs 8-12 minute.
When containing the medium sterilization of water-holding agent, adopted autoclaving 12-18 minute, pressure 1.385 * 10
5Pa, temperature 115-230 ℃.
When adding staining agent in the substratum that contains water-holding agent, elder generation sterilizes after staining agent is mixed with 5% aqueous solution, adds the nutrient agar that contains water-holding agent again.
The technical progress that the present invention obtains is:
Water-holding agent in the bacteria culture medium can be kept the moisture of substratum, avoids the dry accuracy that influences measurement result of culturing gene dehydration; Staining agent can be dyed redness or pink with most of bacterium colony, is convenient to observe detected result; Its preparation method's technological design is reasonable, simple to operation simultaneously.
Embodiment
Below embodiments of the invention are further described:
In the present embodiment, bacteria culture medium is a basic medium with nutrient agar (GB4789.28-1994), also be added with water-holding agent and staining agent in the basic medium, wherein, nutrient agar medium: water: water-holding agent: the weight ratio of staining agent is 38: 1000: 3: 0.01.
Preparation is during bacteria culture medium, should be according to the following steps:
A, preparation nutrient agar;
B, with the nutrient agar for preparing: water: water-holding agent: staining agent was by 38: 1000: 3: after 0.01 weight ratio is weighed, placed apart;
C, load weighted nutrient agar and water are mixed, places after 10 minutes, boil, make fully and dissolve every the asbestos wire net low baking temperature;
D, water-holding agent is ground into 100 purpose particles after, add in the nutrient agar for preparing in the above-mentioned steps, stirred 10 minutes;
E, the substratum that contains water-holding agent to preparing in the d step adopted autoclaving 15 minutes, pressure 1.385 * 10
5Pa, 125 ℃ of temperature.
F, sterilize after load weighted staining agent is mixed with 5% aqueous solution, add in the substratum that contains water-holding agent for preparing in the e step, promptly obtain bacteria culture medium.
The modified-cellulose class High hydrophilous resin that water-holding agent can select for use Beijing Institute of Technology to produce, staining agent can be selected the PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine for use.
The prepared bacteria culture medium of the present invention can place check-out console, plane culture dish or other culture medium carrier.
Claims (9)
1, a kind of bacteria culture medium, it is basic medium with the nutrient agar, it is characterized in that also being added with in the basic medium water-holding agent and staining agent, wherein, nutrient agar medium: water: water-holding agent: the weight ratio of staining agent is 36-40: 1000: 0.1-5: 0.01-0.1.
2, bacteria culture medium according to claim 1 is characterized in that nutrient agar medium: water: water-holding agent: the weight ratio of staining agent is 36-40: 1000: 0.1-1: 0.01-0.1.
3, bacteria culture medium according to claim 1 and 2, it is characterized in that water-holding agent selects for use starch graft copolymer vinylformic acid super strength water absorbent, starch graft copolymer acrylate super strength water absorbent, modified-cellulose class High hydrophilous resin or acryloyl derivative and inorganics to be combined into High hydrophilous resin, staining agent select for use the PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine, Congo red, the PHENOL 99.8 MIN ((CARBOLIC ACID)) sargassun is red, Viola crystallina, methyl orchid or TCC.
4, the preparation method of bacteria culture medium according to claim 1 is characterized in that it comprises following step:
A, earlier with nutrient agar medium: water: water-holding agent: staining agent is by 36-40: 1000: 0.1-5: after the weight ratio of 0.01-0.1 is weighed, placed apart;
B, load weighted nutrient agar medium and water are mixed with nutrient agar;
Add load weighted water-holding agent in c, the nutrient agar that in above-mentioned steps, prepares;
D, the substratum that contains water-holding agent for preparing in the c step is sterilized;
After the staining agent sterilization of e, weighing, add in the substratum of d step preparation, promptly make bacteria culture medium.
5, the preparation method of bacteria culture medium according to claim 4, it is characterized in that nutrient agar medium: water: water-holding agent: staining agent is pressed 36-40: 1000: 0.1-1: the weight ratio weighing of 0.01-0.1.
6, the preparation method of bacteria culture medium according to claim 4 is characterized in that earlier nutrient agar medium being mixed with water placing 8-12 minute, heats then, and nutrient agar medium is fully dissolved.
7, the preparation method of bacteria culture medium according to claim 4, when it is characterized in that adding water-holding agent, elder generation adds in the nutrient agar after water-holding agent is ground into 80-100 purpose particle again, stirs 8-12 minute.
8, the preparation method of bacteria culture medium according to claim 4 is characterized in that when containing the medium sterilization of water-holding agent, adopts autoclaving 12-18 minute, pressure 1.385 * 10
5Pa, temperature 115-230 ℃.
9, the preparation method of bacteria culture medium according to claim 4, when it is characterized in that adding staining agent in the substratum that contains water-holding agent, elder generation sterilizes after staining agent is mixed with 5% aqueous solution, adds the nutrient agar that contains water-holding agent again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012136 CN1234848C (en) | 2004-01-29 | 2004-01-29 | Bacterium culture medium and preparation method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012136 CN1234848C (en) | 2004-01-29 | 2004-01-29 | Bacterium culture medium and preparation method therefor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1557943A CN1557943A (en) | 2004-12-29 |
| CN1234848C true CN1234848C (en) | 2006-01-04 |
Family
ID=34350967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410012136 Expired - Fee Related CN1234848C (en) | 2004-01-29 | 2004-01-29 | Bacterium culture medium and preparation method therefor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1234848C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492765A (en) * | 2011-12-13 | 2012-06-13 | 江门市凯林贸易有限公司 | Agar culture medium and preparation method thereof |
| CN103091145B (en) * | 2013-01-30 | 2015-04-29 | 中国人民解放军军事医学科学院生物工程研究所 | Method for staining microorganisms |
| WO2021211845A1 (en) * | 2020-04-15 | 2021-10-21 | Rapid Micro Biosystems, Inc. | Attenuated-background microbiological nutrient media and methods of using the same |
-
2004
- 2004-01-29 CN CN 200410012136 patent/CN1234848C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1557943A (en) | 2004-12-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102091329B (en) | Preparation method of inactivated porcine parvovirus vaccine and product thereof | |
| CN113234791B (en) | Count detection method of bacillus coagulans | |
| CN1234848C (en) | Bacterium culture medium and preparation method therefor | |
| CN115261262B (en) | Lactobacillus plantarum HSF-LAB-1303 and application thereof | |
| Yin et al. | Economic production of probiotics from kitchen waste | |
| CN104694421A (en) | Bacteria culture medium and preparation method thereof | |
| CN114250196A (en) | Exosome amplification and storage method | |
| CN110124104B (en) | Composition for preparing myocardial patch and application thereof | |
| CN102516473A (en) | Copolymerized nano composite hydrogel for intelligent separation of cell sheets as well as preparation method and application thereof | |
| CN110358801A (en) | A kind of fermented by yeast obtains the method and its application of tridecanoic peptide | |
| CN106190898A (en) | A kind of industrialization liquid fermentation method of the bacillus cereus JZBC1 dissolving pond dinoflagellate | |
| CN106148479B (en) | The detection method of microorganism in grease | |
| CN113637635B (en) | Method for dissolving microcarrier agglomeration state in microcarrier cell culture system | |
| CN106418127B (en) | A kind of method of beta-conglycinin in elimination fermented bean dregs | |
| Wu et al. | Assessment of various carbon sources and nutrient feeding strategies for Panax ginseng cell culture | |
| CN112695018B (en) | Method for improving output of transglutaminase produced by fermentation | |
| CN113350332B (en) | Medicine for preventing and treating diabetes and application thereof | |
| CN106191209B (en) | A method of detection Starch in Tobacco degrading microorganism | |
| CN110201233B (en) | Method for preparing myocardial patch and application thereof | |
| CN107242026A (en) | A kind of cultural method and its culture medium of white Antrodia camphorata fructification | |
| CN104277977B (en) | A kind of preparation method of aspergillus niger spore suspension and the storage method of aspergillus niger spore | |
| CN109627104B (en) | Water-soluble fertilizer for fruit tree planting and preparation method and application thereof | |
| CN107326016B (en) | Method for producing siniperca chuatsi infectious spleen and kidney necrosis virus and mandarin fish rhabdovirus by microcarrier suspension culture of CPB cells | |
| CN104529661B (en) | A kind of vegetable transplanting root dipping microorganism formulation | |
| CN105749308B (en) | A kind of sterilizing methods of edible fungus species fluid nutrient medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |