CN1228348C - Method for producing pumpkin polysaccharide through membrane isolation technique - Google Patents
Method for producing pumpkin polysaccharide through membrane isolation technique Download PDFInfo
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- CN1228348C CN1228348C CN 200310111322 CN200310111322A CN1228348C CN 1228348 C CN1228348 C CN 1228348C CN 200310111322 CN200310111322 CN 200310111322 CN 200310111322 A CN200310111322 A CN 200310111322A CN 1228348 C CN1228348 C CN 1228348C
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Abstract
The present invention relates to a method for preparing pumpkin polysaccharide through a membrane isolation technology. A membrane isolation technology and a freeze drying technology are adopted to prepare and process pumpkin polysaccharide. Pumpkins are crushed and milled, juice is obtained, the pumpkin juice is filtered, pumpkin polysaccharide is separated from the pumpkin juice with a micro-filtration membrane, membranes which have different molecular weight, and a reverse osmosis concentration membrane, and through freeze drying operation, pumpkin polysaccharide powder products are obtained. Polysaccharide parts whose molecular weight is from 30000 to 10000 are collected. The pumpkin polysaccharide has the obvious activity of hypoglycemic effect. The present invention does not use any heating mode for extraction, concentration and dryness. The operation is carried out at the normal temperature and the low temperature. The original flavor and the biological activity of pumpkins are integrally kept. According to the magnitude of the molecular weight of the pumpkin polysaccharide, membranes which have different molecular interception are selected for separation. Simultaneously, the freeze drying technology provides the reliable guarantee for realizing the separation technology of biologically active material. The product of the present invention has the characteristics that the flavor is fragrant, the cold water solubility is good, and the biological activity of the pumpkin polysaccharide can not be destroyed. The present invention is suitable for industrial scale preparation.
Description
Technical field:
The present invention relates to a kind of preparation method of polyose, especially a kind of membrane separation technique production method of squash polyoses.
Background technology:
The laboratory uses separation method to be the poach extraction at present, and is concentrated, alcohol precipitation, and throw out is dissolved in water, and removes protein with chemical method, isolates water-soluble squash polyoses with Portugal's coagel post then.
Present industrial method.Extract with poach, be concentrated into thickly, add Zulkovsky starch, heat drying can get the keen-witted and capable powder of solubility pumpkin.
More than two kinds of methods, all pass through the heating poach and extract, the active substance of the destructible that is heated in the pumpkin is had infringement, reduced its biological activity.And the application cost of laboratory method is higher, and the product specification of industrial process is lower.But more supporting with the method that poach extracts with the equipment of Chinese medicine production at present, less investment.
Summary of the invention:
Purpose of the present invention is exactly to solve the problem that the biologically active substance that keeps pumpkin aborning is not damaged and can be carried out large-scale production.
The technical solution used in the present invention is: adopt membrane separation technique, freeze drying process carries out the process for processing of squash polyoses.Be about to pumpkin fragmentation, mill juice, Sucus Cucurbitae moschatae is filtered the back with the film of microfiltration membrane, different molecular weight, and reverse osmosis concentration film, squash polyoses is separated, lyophilize promptly gets the squash polyoses power-product then, whole process of production need not heat, avoided amylaceous gelatinization reaction, be easy to squash polyoses is separated.The polysaccharide of collecting molecular weight 30000～10000 partly has remarkable hypoglycemic activity.
Advantage of the present invention and positively effect:
(1) the present invention need not go extraction, concentrated, dry by any type of heating, and the normal temperature cold condition is operation down.Integral body has kept original local flavor of pumpkin and biological activity.
(2) the present invention adopt have international most advanced level the molecular separation technology---membrane separation technique is separated, according to squash polyoses molecular weight size, select the different film of molecular retention amount to separate, advanced advantage possesses skills.
(3) the present invention adopts Freeze Drying Technique to carry out product drying, organically combines with membrane separation technique, for the isolation technique that realizes biologically active substance provides reliable assurance.
(4) this product characteristics is a local flavor delicate fragrance, and cold water solubility is better.Can not destroy the biological activity of squash polyoses.
Embodiment:
1, the squash polyoses technological process of production:
Pumpkin → fragmentation → add water mill slurry → coarse filtration → supercentrifuge filtration (or Plate Filtration) → micro-filtration or ultrafiltration → nanofiltration or reverse osmosis to concentrate → lyophilize → preparation.
2, technological operation requires:
(1) pumpkin fragmentation: because the pumpkin shape is inconsistent, and individuality is bigger, therefore needs with just descending the operation in step after the pumpkin fragmentation pumpkin to be broken seed, chopping.
(2) add the water mill slurry: squash polyoses is contained in the pumpkin fruit, needs by defibrination it to be extracted, and therefore adds an amount of water, and to grind pumpkin to such an extent that more carefully be as the criterion, general amount of water is about pumpkin: water=1: 1～1.5 are milled to juice with pumpkin fruit.
(3) coarse filtration: with filter cloth or strainer filtering, remove pulp fraction, fruit juice is waited until next step processing.
(4) whizzer filters or Plate Filtration: the rapid fruit juice of previous step filters through supercentrifuge again, to remove thinner fiber or pulp particle, for next step micro-filtration is got ready.
(5) micro-filtration: the previous step Sucus Cucurbitae moschatae is removed bacterium by micro-filtration, makes product reach hygienic standard.
(6) membrane sepn: through the Sucus Cucurbitae moschatae of micro-filtration, with the separable squash polyoses of the film of different molecular weight to different size.
1. earlier with the ultra-filtration membrane ultrafiltration of 30000 molecular weight, and then with the ultra-filtration membrane ultrafiltration of 10000 molecular weight, the collection molecular weight is the ultrafiltrated of 30000～10000 parts, and concentrate drying gets squash polyoses content greater than 50%, the yield of product about 0.5 ‰.
2. earlier with the ultra-filtration membrane of 30000 molecular weight, use the membrane ultrafiltration of 1000 molecular weight then, collecting molecular weight is 30000～1000 parts, and concentrate drying gets squash polyoses content greater than 30%, the yield of product about 0.1 ‰.
3. first micro-filtration carries out nanofiltration then and concentrates, and microfiltration membrane is 0.02～0.045 μ m, and the polysaccharide part of micro-filtration to solution between the nanofiltration collected in nanofiltration membrane molecular weight 〉=150, and concentrate drying gets squash polyoses content greater than 20%, product yield about 0.2%.
4. filter with micro-filtration earlier, use the ultra-filtration membrane ultrafiltration of 20000 molecular weight then, use the ultra-filtration membrane ultrafiltration of 10000 molecular weight at last, collect molecular weight 20000～10000 parts, squash polyoses content is greater than 70% (about 80%), product yield about 0.2 ‰.
(7) lyophilize: freezing dry process is to reach earlier below the chill point-45 ℃, distillation then, and sublimation temperature is no more than 40 ℃.Lyophilize obtains work in-process.
(8) preparation: what obtain after the lyophilize is the squash polyoses power-product, it can be mixed with other types of functionality food.As squash polyoses oral liquid, squash polyoses capsule, squash polyoses lozenge etc.
Embodiment:
Example 1: charging capacity pumpkin 310kg, shred, remove seed, add water 350kg, defibrination, filtration, micro-filtration, last nanofiltration is to soluble solid 11Brix °, microfiltration membrane is 0.02～0.045 μ m, nanofiltration membrane molecular weight 〉=150, lyophilize, freezing dry process is to reach earlier below the chill point-45 ℃, distillation then, sublimation temperature is no more than 40 ℃.
Example 2: charging capacity pumpkin 230kg, shred, go seed, add water 350kg, defibrination, filtration, supercentrifuge filter, then the film by 30000 molecular weight, after the film of 10000 molecular weight.Collect the ultrafiltrated of 30000～10000 molecular weight, reverse osmosis concentration, the concentrated solution lyophilize, can get squash polyoses content is 57.1%, yield is 0.5 ‰ product.
Micro-filtration, last nanofiltration is to soluble solid 11Brix °, and microfiltration membrane is 0.02～0.045 μ m, nanofiltration membrane molecular weight 〉=150, lyophilize, freezing dry process is to reach earlier below the chill point-45 ℃, distillation then, sublimation temperature is no more than 40 ℃.
Product can be made the squash polyoses oral liquid.With micro-filtration then the spissated concentrated solution Brix of nanofiltration ° be 10 °, carry out the micro-filtration can again, 10ml/ props up, the check, product squash polyoses oral liquid.
Claims (5)
1, a kind of preparation method of squash polyoses is characterized in that:
With pumpkin break seed, shred, add water mill slurry, filter cloth or strainer filtering, centrifuging, employing elder generation's micro-filtration or ultrafiltration, nanofiltration reverse osmosis concentration again, process need not heat before this; Collect the polysaccharide filtrate of molecular weight 30000～10000, lyophilize obtains the squash polyoses powder-product;
Described lyophilize is meant and reaches earlier below the chill point-45 ℃ that resublime is no more than under 40 ℃ of conditions dry.
2, the preparation method of squash polyoses according to claim 1 is characterized in that: first micro-filtration carries out nanofiltration then and concentrates, and collects the polysaccharide part of micro-filtration to solution between the nanofiltration; The microfiltration membrane aperture is 0.02～0.045 μ m, nanofiltration membrane molecular weight 〉=150.
3, the preparation method of squash polyoses according to claim 1 is characterized in that: earlier with the ultra-filtration membrane ultrafiltration of 30000 molecular weight, and then with the ultra-filtration membrane ultrafiltration of 10000 molecular weight, the collection molecular weight is the ultrafiltrated of 30000～10000 parts.
4, the preparation method of squash polyoses according to claim 1, it is characterized in that: filter with micro-filtration earlier, use the ultra-filtration membrane ultrafiltration of 20000 molecular weight then, use the ultra-filtration membrane ultrafiltration of 10000 molecular weight at last, collecting molecular weight is the ultrafiltrated of 20000～10000 parts.
5, the preparation method of squash polyoses according to claim 1 is characterized in that: with the ultra-filtration membrane ultrafiltration of 30000 molecular weight, use the ultra-filtration membrane ultrafiltration of 1000 molecular weight then earlier, collecting molecular weight is the ultrafiltrated of 30000～1000 parts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310111322 CN1228348C (en) | 2003-10-31 | 2003-10-31 | Method for producing pumpkin polysaccharide through membrane isolation technique |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310111322 CN1228348C (en) | 2003-10-31 | 2003-10-31 | Method for producing pumpkin polysaccharide through membrane isolation technique |
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| Publication Number | Publication Date |
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| CN1539852A CN1539852A (en) | 2004-10-27 |
| CN1228348C true CN1228348C (en) | 2005-11-23 |
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| CN 200310111322 Expired - Fee Related CN1228348C (en) | 2003-10-31 | 2003-10-31 | Method for producing pumpkin polysaccharide through membrane isolation technique |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101220100B (en) * | 2007-01-10 | 2010-05-12 | 兰州大学 | Separation and purification method for squash polyoses and use of obtained component |
| CN104762356B (en) * | 2015-04-08 | 2018-07-17 | 西北农林科技大学 | A kind of pumpkin leaf polyose and polypeptide and preparation method thereof |
| CN107383228A (en) * | 2017-08-18 | 2017-11-24 | 合肥丰洁生物科技有限公司 | A kind of method for extracting high purity of tremella polysaccharides |
| CN107927473A (en) * | 2017-11-24 | 2018-04-20 | 王成江 | A kind of efficient multi items deep working method of fresh burdock |
| CN112294691A (en) * | 2020-11-04 | 2021-02-02 | 赵玉龙 | Pumpkin-extracted firming anti-wrinkle molecular mask and preparation method thereof |
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Granted publication date: 20051123 Termination date: 20091130 |