CN1221110A - Quick test method for adulteration of propolis - Google Patents
Quick test method for adulteration of propolis Download PDFInfo
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- CN1221110A CN1221110A CN 98124545 CN98124545A CN1221110A CN 1221110 A CN1221110 A CN 1221110A CN 98124545 CN98124545 CN 98124545 CN 98124545 A CN98124545 A CN 98124545A CN 1221110 A CN1221110 A CN 1221110A
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- propolis
- adulteration
- test method
- quick test
- alcoholic
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Abstract
A method for quickly detecting the adulterated propolis with a simple analytic instrument features that hydrochloric acid and magnesium powder are added to alcoholic solution of propolis product to be detected, after shaking it shows red, which is positive reaction, then add sulfuric acid and potassium permanganate to the alcoholic solutionof the propolis product, the time needed for decolouring it is recorded, and the result is obtained by looking up table.
Description
The present invention relates to the method that the fast detecting natural propolis mixes foreign matter.
The present invention is after the finder presides over the NSFC's project of bearing " bee product chemistry and development and use research " end, draft for the Ministry of Commerce and invent on the basis of " People's Republic of China's propolis industry standard ", the method that does not adopt fast measuring propolis of the present invention to mingle at home as yet, the reference paper of relevant this technology has:
1, People's Republic of China's industry standard " propolis " SB/F 10096-92.
2, CRITERIA FOR QUALITY OF PROPOLIS, THEXXXLLLrd INTERNATIONAL APICULTURAL CONGRESS.APIMONDIA-PUBLISHING HOUSE BUCHAREST.ROMANIA. (Beijing international bee-keeping conference collection of thesis in 1993).
Propolis is that the resin on the honeybee herborization body mixes the solid jelly with viscosity with formation such as its mandibular gland secretion and beeswaxs, it is natural broad-spectrum antibiotic and innate immunity reinforcing agent, and numerous diseases such as diabetes, enterogastritis, mouth disease, herpes zoster are all had peculiar effect.Aspects such as medicine, health products, cosmetics and food preservative now have been widely used in.China is bee-keeping big country, and the output of propolis and export volume all belong to the No. 1 in the world.But propolis constantly was exploited in recent years, and requirement is increasing, and procurement price is also more and more higher, however mingle but more and more in vogue, almost have more than 3/4 propolis all by the people for mingling, What is more mixes the material of pitch etc. and so on, no small harm is done.The international market is also extruded by states such as Brazil.Conventional method testing amount is big, and the time is long, can't differentiate that to those a small amount of, propolis wide in variety therefore, it is imperative doing a method easy, that whether the fast measuring propolis is mingled when purchase.
Purpose of the present invention just provide a kind of easy, whether method detects propolis and mingles fast.
The content of quick test method for adulteration of propolis:
Qualitative reaction: will be in refrigerator a little frozen propolis grind, after taking by weighing 100 milliliter of 95% dissolve with ethanol of 1 gram adding, get 2 milliliters in test tube, drip with the salt acid number, add 0.1 gram magnesium powder, shake test tube, mixed liquor takes on a red color, positive (+) reaction, show flavonoid content 〉=4.5%: do not develop the color, negative (-) reaction shows its content＜4.5%, has and mingles.
Oxidization time: get and grind propolis 1 gram, add 25 milliliter of 95% ethanol, after the dissolving,, fully shake up, filter with 500 milliliters of distilled water dilutings.Get 10 milliliters of filtrates in test tube, add 1 milliliter of 20% sulfuric acid, shook 1 minute, add 1 0.1moL/L liquor potassic permanganate (aubergine) again, start stopwatch simultaneously, write down the time of fading.Reduzate fades soon more, otherwise opposite, but must not＜22 seconds.
, and do artificial simulation and mingle test from national different regions or 120 parts in the propolis sample gathered of areal Various Seasonal, different seeds according to us, record and the results are shown in Table, tabling look-up just comes into plain view.
| Qualitative reaction (+) (-) | Oxidization time (second) | Conclusion |
| ????(+) ????(+) ????(-) | ????≤22 ????＞22 ????＞22 | The certified products off standard is mingled |
The inventive method is simplified all samples and instrument, is loaded in the little case, is easy to carry.
The inventive method is simpler than existing method, easily grasps, and the time is short, accurately.Its method uses instrument simple after simplifying, and only spends 400～500 yuan just can purchase together, is convenient to basic unit's purchase and uses.
Claims (2)
1, the method for quick mingled of a kind of propolis, its predicting relation is as follows:
Qualitative reaction (+) (one) Oxidization time (second) Conclusion
????(+) ????(+) ????(-) ????≤22 ????＞22 ????＞22 The certified products off standard is mingled
2, according to claim 1, wherein amount of samples is 1 gram.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98124545 CN1221110A (en) | 1998-11-12 | 1998-11-12 | Quick test method for adulteration of propolis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98124545 CN1221110A (en) | 1998-11-12 | 1998-11-12 | Quick test method for adulteration of propolis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1221110A true CN1221110A (en) | 1999-06-30 |
Family
ID=5228697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 98124545 Pending CN1221110A (en) | 1998-11-12 | 1998-11-12 | Quick test method for adulteration of propolis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1221110A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100351628C (en) * | 2005-07-29 | 2007-11-28 | 浙江蜂之语蜂业集团有限公司 | Method of identifying true and false of propolis using liquid phase finger print atlas |
-
1998
- 1998-11-12 CN CN 98124545 patent/CN1221110A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100351628C (en) * | 2005-07-29 | 2007-11-28 | 浙江蜂之语蜂业集团有限公司 | Method of identifying true and false of propolis using liquid phase finger print atlas |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |