CN1645114A - Nitrate and nitrite rapid testing paper and use thereof - Google Patents
Nitrate and nitrite rapid testing paper and use thereof Download PDFInfo
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- CN1645114A CN1645114A CN 200510048918 CN200510048918A CN1645114A CN 1645114 A CN1645114 A CN 1645114A CN 200510048918 CN200510048918 CN 200510048918 CN 200510048918 A CN200510048918 A CN 200510048918A CN 1645114 A CN1645114 A CN 1645114A
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Abstract
A test paper for fast measuring out nitrate and nitrite is composed of colour display test paper soaked with mixture in the same volume of diazotization reagent and coupling reagent, and reaction test paper coated with nitrate reducer, the test paper can provide quick detection of nitrate and nitrite contained in food or vegetables.
Description
Technical field
The invention belongs to detection technique, relate to the technology that detects nitrate, content of nitrite, relate in particular to the fast measuring test paper and the application thereof that detect nitrate, content of nitrite in the food (vegetables).
Background technology
Along with growth in the living standard, people no longer only pay attention to consumption quantity to the demand of vegetables, and more pay attention to quality.Production is at present gone up a large amount of applied nitrogens and is caused the soil nitrate nitrogen content generally higher, is very easily accumulated in its body after being absorbed by crop.There are some researches show, vegetables are crops of a kind of easy enrichment nitrate, the nitrate major part (accounting for 70%-80%) that human body is taken in is from vegetables, reducible one-tenth nitrite under the effect of nitrate bacterium in human body, nitrite can make the oxygen carrying capability of blood descend, thereby causes methemoglobinemia.On the other hand, nitrite also can react with the secondary amine (secondary amine, tertiary amine, acid amides and amino acid) in the compositions such as other food of human intake, medical product, remains of pesticide, (pH=3) forms powerful carcinogenic substance---nitrosamine in gastral cavity, thereby bring out the digestive system canceration, this has constituted potential threat to human health.Therefore, how to reduce nitrate in the vegetables, nitrite accumulation, how allowing numerous citizen be able to eat " quality-assured vegetables " has early become government agencies at all levels and agricultural science and technology worker key subjects anxious to be solved.
The main direction that current vegetables produce is the development pollution-free vegetable, limit the quantity of (GB 19338-2003) and agricultural product security quality pollution-free vegetable safety requirements (GB18406.1-2001) requirement according to nitrate in the up-to-date vegetables of China, several main vegetables that China's consumption of resident amount is bigger (especially leaf vegetables, root vegetables) nitrate content exceeds standard greatly, this phenomenon does not attract great attention so far as yet, does not more have vegetable nitrate testing tool fast and effectively.Therefore, must seek fast, accurately, detecting instrument and technology cheaply, be used for detecting the content of nitrate in the vegetables, nitrite.
The method of nitrate and nitrite of measuring at present in the vegetables has multiple, as spectroscopic methodology, chromatography, electrophoresis, polarography etc., but needs specialized equipment mostly, and mensuration process complexity, and the time is long.By retrieval as can be known: " quick detection test paper of nitrite, nitrate and detection method thereof in the vegetables " (application number: 0311666.6 of invention such as Xie Zenghong, publication number: CN 1445531A) though also be nitrate and method of rapid determination for nitrite in a kind of vegetables, but it only is to judge whether content of nitrite surpasses 8mg/kg in the vegetables, whether nitrate content surpasses 10mg/kg, can't judge in view of the above whether nitrate and content of nitrite in the vegetables surpass the limit standard of national regulation.In addition, when detecting nitrate, also need reaction vessel and add reductive agent, more loaded down with trivial details, not too be fit to numerous crowds and use at any time.
Summary of the invention
The objective of the invention is to overcome above-mentioned the deficiencies in the prior art, provide cheap, easy to use, without any need for the fast measuring test paper of instrument, can determine in the vegetables roughly content of nitrate, nitrite simultaneously, and judge whether to surpass the limit standard of national regulation.
Nitrate provided by the invention, nitrite rapid testing paper are by colour developing test paper and the combination of reaction test paper, the colour developing test paper is the mixed liquor that is soaked with isopyknic diazo reagent and coupling reagent, and the reaction test paper scribbles nitrate metallic reducing agents such as zinc, copper, magnesium, cadmium.
Mensuration test paper provided by the invention prepares by the following method:
(1) preparation of reaction test paper: will open the filter paper acidifying greatly earlier, and divide tow sides, and coat the nitrate metallic reducing agent at the reverse side of filter paper then, the test paper of making is white in color, and the lucifuge cryopreservation is cut into the fritter of 1 * 0.8 centimetre (long * wide) during use.
(2) preparation of colour developing test paper: will open greatly in the mixed liquor that filter paper immerses isopyknic diazo reagent and coupling reagent.The baking oven of sending into 60 ℃-80 ℃ after wetting through 3-5 minute immediately is interior with its oven dry.The test paper of making is white in color, and the lucifuge cryopreservation is cut into the fritter of 1 * 0.8 centimetre (long * wide) during use.
(3) nitrate, nitrite are measured the preparation of test paper: with double faced adhesive tape colour developing test paper fritter is sticked on the lower end of 7.5 * 1.0 centimetres white plastic bar, interval 0.5 centimeters reverse side is pasted the reaction test paper downwards thereon.With nitrate, the nitrite test paper for preparing, referring to accompanying drawing 1, put into polybag, brown bottle is put in sealing, and low-temperature dark is preserved in refrigerator.
Mensuration test paper provided by the invention, the applications of nitrate, content of nitrite in fast detecting food (vegetables).
Nitrate provided by the invention, nitrite rapid testing paper are furnished with nitrate standard color range, nitrite standard color range, wherein nitrate standard color range is 6 color lumps, represent 6 nitrate content grades respectively, promptly 440,750,1200,2000,2500,3000mg kg
-1Fresh weight; Nitrite standard color range is 3 color lumps, represents 3 content of nitrite grades respectively, promptly 2,4,6, mg kg
-1Fresh weight.
The principle that the present invention detects nitrite is: under acid condition, after nitrite and the sulfanilamide (SN) diazotising, generate the aubergine compound with two hydrochloric acid-1-naphthodiamide generation coupling reaction again in the vegetables.
The principle that the present invention detects nitrate is: under acid condition, nitrate is reduced agent and is reduced into nitrite, and the principle by above-mentioned detection nitrite detects nitrate then.
Description of drawings
Fig. 1 is test paper synoptic diagram of the present invention (1. 2. representative colour developing test paper represents the reaction test paper).
Fig. 2 is nitrate standard color range (mg kg
-1Fresh weight).
Fig. 3 is nitrite standard color range (mg kg
-1Fresh weight).
Embodiment
The present invention is described further in conjunction with the embodiments.
The preparation method of embodiment 1 test paper of the present invention
The preparation of reaction test paper: will open the filter paper acidifying greatly earlier, divide tow sides, coat nitrate metallic reducing agents such as zinc, copper, magnesium, cadmium then at the reverse side of filter paper, the test paper front of making is white in color, the lucifuge cryopreservation is cut into the fritter of 1 * 0.8 centimetre (long * wide) during use.
The preparation of colour developing test paper: will open greatly in the mixed liquor that filter paper immerses isopyknic diazo reagent and coupling reagent, and send into immediately after wetting through 3-5 minute in 60 ℃-80 ℃ the baking oven with its oven dry.The test paper of making is white in color, and the lucifuge cryopreservation is cut into the fritter of 1 * 0.8 centimetre (long * wide) during use.
Nitrate, nitrite are measured the preparation of test paper: with double faced adhesive tape colour developing test paper fritter is sticked on the lower end of 7.5 * 0.8 centimetres white plastic bar, interval 0.5 centimeters reverse side is pasted the reaction test paper downwards thereon.With nitrate, the nitrite test paper for preparing, referring to accompanying drawing 1, put into polybag, brown bottle is put in sealing, and low-temperature dark is preserved in refrigerator.
Main agents of the present invention preparation is: diazo reagent: take by weighing sulfanilamide (SN) 0.5-1.5g and 30-50g tartrate is dissolved in the 100mL distilled water, store in the brown reagent bottle, put into 4 ℃ of refrigerators and preserve; Coupling reagent: take by weighing 0.1-0.3g two hydrochloric acid-1-naphthodiamide and 10-20g tartrate and be dissolved in the 100mL distilled water, store in the brown reagent bottle, put into 4 ℃ of refrigerators and preserve.
(1) content of nitrite: get vegetables juice number droplet (1-2 drips and is advisable) and drop on the colour developing test paper, observe the test paper change color after 1-2 minute, compare with the standard color range, estimate content of nitrite, judge whether to exceed standard, the standard that exceeds standard is limited the quantity of (with NaNO according to nitrite in agricultural product security quality pollution-free vegetable safety requirements (GB18406.1-2001) vegetables of implementing on the issue in 6 days Augusts calendar year 2001 of The People's Republic of China's national quality supervision quarantine general bureau, October 1 calendar year 2001
2Meter) :≤4
(2) nitrate content: taking juice number droplet (1-2 drips and is advisable) drops on the reaction test paper, erect test paper after 1-3 minute, allow the reactant liquor following current to lower end colour developing test paper, carry out chromogenic reaction, observe its change color, compare with the standard color range after 1-2 minute, estimate the nitrate content scope, judge whether to exceed standard, the standard that exceeds standard is according to the issue on October 9th, 2003 of The People's Republic of China's national quality supervision quarantine general bureau, nitrate is limited the quantity of (GB 19338-2003) in the vegetables of implementing on May 1st, 2004, sees table 1 for details.
Nitrate limit the quantity of (GB 19338-2003) in table 1 vegetables
Vegetables classification nitrate is (with NO
3 -Meter)/(mg kg
-1Fresh weight)
Solanberry class, melon, beans≤440
Stem dish class≤1200
Root vegetables≤2500
Leaf vegetables≤3000
Embodiment 3 application examples
Compare with laboratory conventional method of analysis (standard law), all kinds of nitrate salts content of vegetable are (with NO
3 -The meter) with content of nitrite (with NaNO
2Meter) test paper method and standard law are measured, and measurement result is referring to table 2.
Table 2 test paper method and standard law comparative unit (mg kg
-1)
Vegetable variety standard law measured value test paper method measured value standard law measured value test paper method measured value
(NO
3 -) (NaNO
2)
Pakchoi 2,597 2,300 1.27 2
Pakchoi 4250＞3000 (exceeding standard) 4.27＞4 (exceeding standard)
Wild cabbage 2,035 2000
Rape 854 800
Spinach 2,914 3,000 3.35 3
Cucumber 645 500 (exceeding standard)
Asparagus lettuce 1,769 1,600 3.07 3
Press reaction test paper preparation employing nitrate zinc powder reduction agent among the embodiment 1, record the pakchoi nitrate content (with NO
3 -Meter) as table 3.
Table 3 nitrate zinc powder reduction test paper (the mg kg of measurement result unit
-1)
Sample number estimated value sample number estimated value
1 3000 6 2500
2 2000 7 2725
3＞3000 (exceeding standard) 8 600
4 3,000 9＞3000 (exceeding standard)
5 1200 10 2225
Embodiment 5 nitrate copper powder reduction tests
Press reaction test paper preparation employing nitrate copper powder reductive agent among the embodiment 1, record the wild cabbage nitrate content (with NO
3 -Meter) as table 4.
Table 4 nitrate copper powder reduction test paper (the mg kg of measurement result unit
-1)
Sample number estimated value sample number estimated value
1＞3000 (exceeding standard) 6 750
2 3000 7 3000
3 2500 8 2725
4 1,200 9＞3000 (exceeding standard)
5 750 10 1600
Press reaction test paper preparation employing nitrate magnesium powder reductive agent among the embodiment 1, record the pakchoi nitrate content (with NO
3 -Meter) as table 5.
Table 5 nitrate magnesium powder reduction test paper (the mg kg of measurement result unit
-1)
Sample number estimated value sample number estimated value
1 2000 6 2500
2 3,000 7＞3000 (exceeding standard)
3 1200 8 2500
4 1,200 9＞3000 (exceeding standard)
5＞3000 (exceeding standard) 10 1200
Embodiment 7 nitrate cadmium powder reduction tests
Press reaction test paper preparation employing nitrate cadmium powder reductive agent among the embodiment 1, record the pakchoi nitrate content (with NO
3 -Meter) as table 6.
Table 6 nitrate cadmium powder reduction test paper (the mg kg of measurement result unit
-1)
Sample number estimated value sample number estimated value
1 3000 6 1200
2 2000 7 2000
3 1,200 8＞3000 (exceeding standard)
4 2,000 9＞3000 (exceeding standard)
5 2000 10 750
Embodiment 8 nitrate zinc-magnesium powder mixing reduction tests
Press reaction test paper preparation employing nitrate zinc-magnesium powder mixed in equal amounts reductive agent among the embodiment 1, record the pakchoi nitrate content (with NO
3 -Meter) as table 7.
Table 7 nitrate cadmium powder reduction test paper (the mg kg of measurement result unit
-1)
Sample number estimated value sample number estimated value
1＞3000 (exceeding standard) 6 2225
2 1200 7 2500
3 2000 8 2000
4 2,500 9＞3000 (exceeding standard)
5 2500 10 1200
Embodiment 9 nitrate zinc-copper powder mixing reduction tests
Press reaction test paper preparation employing nitrate zinc-copper powder mixed in equal amounts reductive agent among the embodiment 1, record the pakchoi nitrate content (with NO
3 -Meter) as table 8.
Table 8 nitrate cadmium powder reduction test paper (the mg kg of measurement result unit
-1)
Sample number estimated value sample number estimated value
1 2000 6 2000
2＞3000 (exceeding standard) 7 3000
3 2500 8 750
4 2000 9 2000
5 2,500 10＞3000 (exceeding standard)
Embodiment 10 nitrate zinc, copper, magnesium, cadmium powder mixing reduction test
Press reaction test paper preparation employing nitrate zinc, copper, magnesium, cadmium powder mixed in equal amounts reductive agent among the embodiment 1, record the pakchoi nitrate content (with NO
3 -Meter) as table 9.
Table 9 nitrate cadmium powder reduction test paper (the mg kg of measurement result unit
-1)
Sample number estimated value sample number estimated value
1 3,000 6＞3000 (exceeding standard)
2 1200 7 2000
3 2000 8 2500
4 2500 9 2225
5＞3000 (exceeding standard) 10 2500
Need not further to elaborate, believe and adopt the disclosed content in front, those skilled in the art can use the present invention to greatest extent.Therefore, the preferred specific embodiments of front should be understood that only to illustrate, but not limits the scope of the invention by any way.
Claims (8)
1. nitrate, nitrite rapid testing paper, it is characterized in that: by colour developing test paper and the combination of reaction test paper, the colour developing test paper is the mixed liquor that is soaked with isopyknic diazo reagent and coupling reagent, and the reaction test paper scribbles nitrate metallic reducing agents such as zinc, copper, magnesium, cadmium.
2. nitrate according to claim 1, nitrite rapid testing paper is characterized in that: preparation by the following method:
(1) preparation of reaction test paper: will open the filter paper acidifying greatly earlier, and divide tow sides, and coat nitrate metallic reducing agents such as zinc, copper, magnesium, cadmium then at the reverse side of filter paper, the test paper of making is white in color, and is cut into long 1 centimetre during use, wide 0.8 centimetre fritter;
(2) preparation of colour developing test paper: will open greatly in the mixed liquor that filter paper immerses isopyknic diazo reagent and coupling reagent, the baking oven of sending into 60 ℃-80 ℃ after wetting through 3-5 minute immediately is interior with its oven dry, the test paper of making is white in color, and is cut into long 1 centimetre during use, wide 0.8 centimetre fritter;
(3) preparation of mensuration test paper: the lower end that colour developing test paper fritter is sticked on 7.5 * 1.0 centimetres white plastic bar with glue, 0.5 centimeters reverse side is pasted the reaction test paper downwards at the interval, with nitrate, the nitrite test paper for preparing, put into polybag, sealing, put brown bottle, low-temperature dark is preserved in refrigerator.
3. nitrate according to claim 1 and 2, nitrite rapid testing paper is characterized in that: diazo reagent is dissolved in the 100mL distilled water by 0.5-1.5g sulfanilamide (SN) and 30-50g tartrate, stores in the brown reagent bottle, puts into 4 ℃ of refrigerators and preserves.
4. nitrate according to claim 1 and 2, nitrite rapid testing paper, it is characterized in that: coupling reagent is dissolved in the 100mL distilled water by 0.1-0.3g two hydrochloric acid-1-naphthodiamide and 10-20g tartrate, store in the brown reagent bottle, put into 4 ℃ of refrigerators and preserve.
5. nitrate according to claim 1 and 2, nitrite rapid testing paper are used in fast detecting Nitrate Content in Food, content of nitrite.
6. nitrate according to claim 1 and 2, nitrite rapid testing paper are used in nitrate, the content of nitrite in the fast detecting vegetables.
7. nitrate according to claim 1 and 2, nitrite rapid testing paper is characterized in that: supporting nitrate standard color range is 6 color lumps, represents 6 nitrate content grades respectively, promptly 440,750,1200,2000,2500,3000mg kg
-1Fresh weight.
8. nitrate according to claim 1 and 2, nitrite rapid testing paper is characterized in that: supporting nitrite standard color range is 3 color lumps, represents 3 content of nitrite grades respectively, promptly 2,4,6, mg kg
-1Fresh weight.
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|---|---|---|---|
| CNB2005100489184A CN100427928C (en) | 2005-01-17 | 2005-01-17 | Nitrate and nitrite rapid testing paper and use thereof |
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|---|---|---|---|
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007041923A1 (en) * | 2005-10-07 | 2007-04-19 | The University Of Hong Kong | Reagents for highly specific detection of peroxynitrite |
| CN102472746A (en) * | 2009-08-04 | 2012-05-23 | 3M创新有限公司 | Method of detecting oxides of nitrogen |
| CN103534590A (en) * | 2011-03-15 | 2014-01-22 | 哈克兰格有限责任公司 | Method of determining a concentration of nitrate |
| CN104330367A (en) * | 2013-11-19 | 2015-02-04 | 上海绿帝环保科技有限公司 | Rapid detection agent for nitrate |
| CN105004714A (en) * | 2015-06-10 | 2015-10-28 | 苏州东辰林达检测技术有限公司 | Detection reagent for nitrate in hot pickled tuber mustard and detection method thereof |
| CN105628634A (en) * | 2016-03-02 | 2016-06-01 | 山东安博仪器股份有限公司 | Reagent for detecting nitrite in food and nitrite detection method |
| CN106525830A (en) * | 2016-11-03 | 2017-03-22 | 百奥森（江苏）食品安全科技有限公司 | Nitrite test paper |
| CN109856123A (en) * | 2018-12-11 | 2019-06-07 | 广东环凯微生物科技有限公司 | A kind of nitrate quick detection reagent and detection method |
| CN110749591A (en) * | 2019-10-26 | 2020-02-04 | 中国科学院新疆理化技术研究所 | Colorimetric detection method for nitrate in explosive |
| CN113418913A (en) * | 2021-06-24 | 2021-09-21 | 神美科技有限公司 | Stable nitrite test paper and preparation method thereof |
| CN113670902A (en) * | 2021-07-02 | 2021-11-19 | 四川师范大学 | Detection agent for rapidly and quantitatively determining nitrite and detection method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3645853A (en) * | 1969-06-24 | 1972-02-29 | Warner Lambert Co | Diagnostic composition and method for the detection of nitrate reduction |
| JPH0619326B2 (en) * | 1987-02-05 | 1994-03-16 | 環境エンジニアリング株式会社 | Method for quantifying inorganic nitrogen |
| CN1040141C (en) * | 1993-04-29 | 1998-10-07 | 付广录 | Reagent paper for monitoring food sanitation and producing process thereof |
| CN1445531A (en) * | 2003-04-26 | 2003-10-01 | 福州大学 | Test paper for fast testing nitrite, azotate in vegetables and its testing method |
| CN1458514A (en) * | 2003-06-11 | 2003-11-26 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Nitrite detection test paper and its preparing method |
-
2005
- 2005-01-17 CN CNB2005100489184A patent/CN100427928C/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007041923A1 (en) * | 2005-10-07 | 2007-04-19 | The University Of Hong Kong | Reagents for highly specific detection of peroxynitrite |
| CN102472746B (en) * | 2009-08-04 | 2014-08-13 | 3M创新有限公司 | Method of detecting oxides of nitrogen |
| CN102472746A (en) * | 2009-08-04 | 2012-05-23 | 3M创新有限公司 | Method of detecting oxides of nitrogen |
| CN103534590B (en) * | 2011-03-15 | 2015-11-25 | 哈克兰格有限责任公司 | Measure the method for nitrate concentration |
| CN103534590A (en) * | 2011-03-15 | 2014-01-22 | 哈克兰格有限责任公司 | Method of determining a concentration of nitrate |
| CN104330367A (en) * | 2013-11-19 | 2015-02-04 | 上海绿帝环保科技有限公司 | Rapid detection agent for nitrate |
| CN104330367B (en) * | 2013-11-19 | 2017-01-18 | 上海绿帝环保科技有限公司 | Rapid detection agent for nitrate |
| CN105004714A (en) * | 2015-06-10 | 2015-10-28 | 苏州东辰林达检测技术有限公司 | Detection reagent for nitrate in hot pickled tuber mustard and detection method thereof |
| CN105628634A (en) * | 2016-03-02 | 2016-06-01 | 山东安博仪器股份有限公司 | Reagent for detecting nitrite in food and nitrite detection method |
| CN106525830A (en) * | 2016-11-03 | 2017-03-22 | 百奥森（江苏）食品安全科技有限公司 | Nitrite test paper |
| CN109856123A (en) * | 2018-12-11 | 2019-06-07 | 广东环凯微生物科技有限公司 | A kind of nitrate quick detection reagent and detection method |
| CN110749591A (en) * | 2019-10-26 | 2020-02-04 | 中国科学院新疆理化技术研究所 | Colorimetric detection method for nitrate in explosive |
| CN113418913A (en) * | 2021-06-24 | 2021-09-21 | 神美科技有限公司 | Stable nitrite test paper and preparation method thereof |
| CN113670902A (en) * | 2021-07-02 | 2021-11-19 | 四川师范大学 | Detection agent for rapidly and quantitatively determining nitrite and detection method thereof |
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|---|---|
| CN100427928C (en) | 2008-10-22 |
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