CN1220169A - Artificial joint coated with bone morphogenesis protein and its making method - Google Patents
Artificial joint coated with bone morphogenesis protein and its making method Download PDFInfo
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- CN1220169A CN1220169A CN 98125674 CN98125674A CN1220169A CN 1220169 A CN1220169 A CN 1220169A CN 98125674 CN98125674 CN 98125674 CN 98125674 A CN98125674 A CN 98125674A CN 1220169 A CN1220169 A CN 1220169A
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- artificial joint
- morphogenetic protein
- bone morphogenetic
- hydroxyapatite
- bone
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Abstract
The present invention relates to an artificial joint coated with bone morphogenetic protein and its preparation method, belonging to the field of biomedical material. In particular, it is an artificial joint on which a layer of hydroxyapatite with thickness of 50-100 micrometers is sprayed, then a layer of bone morphogenetic protein is combined on the hydroxyapatite coating layer. The advantage of the artificial joint made up by adopting said invented method lies in that after the artificial joint is implanted in the human body, the physiological fixation of said artificial joint can be implemented by means of osteogenic effect induced by bone morphogenetic protein so as to effectively solve the difficult problem of that the existent artificial joint implanted in human body is easy to loosen.
Description
The present invention relates to field of biomedical materials, be applicable to that the physiology of artificial joint is fixed.
Artificial joint and functional prosthese implant significant to recovering limb function, and the artificial joint of annual implant into body has reached 1,200,000 examples, has obtained extensive clinical practice.But, the artificial joint that implants, some is improper because of performing the operation, and is fixing not good, and osteoporosis or activity take place loosening greatly, cause the prosthetic replacement to fail, and finally have to implement second operation.Implant the loosening problem in back in order to solve artificial joint, people have adopted at the artificial joint handle and have made the Margarita surface, increase contact surface with body internal skeleton and tissue, improve interface bond strength, in addition, filling bone cement carries out fixing (the seeing " idea that prevents the whole coxa substitution prosthetic loosening ", Chinese journal of orthopedics the 16th the 12nd phase of volume of December in 1996) of artificial joint etc.But these methods are not very effective.
The object of the present invention is to provide a kind of artificial joint and manufacture method thereof that scribbles bone morphogenetic protein, this artificial joint implants the back by the effect of bone morphogenetic protein induced osteogenesis, area of new bone is linked to each other with the body internal skeleton, the physiology of realization artificial joint is fixed, and solves effectively after artificial joint implants in the prior art loosening problem to take place.
Based on above-mentioned purpose, the characteristics in the artificial joint of the present invention are:
At artificial joint surface spraying one deck hydroxyapatite, thickness is 50-100 μ m, and then at hydroxyapatite coating layer surface recombination last layer bone morphogenetic protein, thickness is 0.1-1 μ m.
Above-mentioned artificial joint obtains by following production stage.
(1) process artificial joint with titanium-base alloy or vitallium,
(2) spraying one deck hydroxyapatite is stand-by on described artificial joint, and thickness is 50-100 μ m,
(3) add the phosphate buffer of 1-10% (percent by volume) 10mM in the carbamide of 5-7M, the control acid-base value is PH6-8,
(4) then bone morphogenetic protein is dissolved in the above-mentioned urea liquid, addition is the bone morphogenetic protein or 10 of 0.01-0.05mg/ml natural extract
-5-10
-3The bone morphogenetic protein of mg/ml gene recombinaton forms the urea liquid that contains bone morphogenetic protein,
(5) will be coated with the artificial joint of hydroxyapatite surface coating again, put into the above-mentioned urea liquid that contains bone morphogenetic protein, soak 10-14 hour down at 20-30 ℃, every 2 hours slow agitating solution 1-3 minute, then with the artificial joint taking-up,
(6) be the artificial joint temperature of above-mentioned taking-up that 1-10 ℃, concentration are 85% the careful drip washing of alcoholic solution 2 times, the reuse temperature is that 1-10 ℃, concentration are 100% alcoholic solution drip washing one time, airing, aseptic preservation, stand-by, promptly make the artificial joint of the present invention.
BMP is the english abbreviation of bone morphogenetic protein (Bone Morphogenetic Protein).This albumen has unique induced osteogenesis function, and knochenbruch is connect fast again, lacks the quick Regeneration and Repair of bone, is with a wide range of applications at the orthopaedics and the department of stomatology.
At present, preparation BMP has two kinds of methods: a kind of is to utilize biochemical extraction method directly to extract from animal bone such as cattle, horse, pig, sheep, rabbit, Mus or osteosarcoma, and a kind of is to utilize method of gene recombination directly to prepare.The activation of the induced osteogenesis of the BMP of gene recombinaton is strong, than biochemical extraction method extract high by 10
3-10
6Doubly, though BMP has very strong induced osteogenesis activity, because of the absorption that implants, metabolism is fast, and action time is short.Be difficult to give full play to its induced osteogenesis effect.In addition, BMP does not have the support effect when reparation large scale bone is damaged.Therefore, BMP needs with some carrier material compound in clinical practice, himself obtains slow release and support so that make.
Hydroxyapatite (Hydroxyapatite is called for short HA) is a kind of of calcium orthophosphate base bioceramic, and its chemical formula is Ca
10(PO
4)
6(OH)
2Be that the main inanimate matter that constitutes bone and tooth, the back that implants can form chemical bond with bone and soft tissue on its surface.So have good biological activity and biocompatibility, be the ideal alternative repair materials of human body hard tissue (bone, tooth), be one of ideal carrier material of BMP.BMP and HA is compound, both helped giving full play to of BMP induced osteogenesis effect, the bone repair of HA is strengthened.
Artificial joint is processed by titanium-base alloy or vitallium usually, can obviously improve its biocompatibility at artificial joint surface spraying one deck hydroxyapatite.If at the compound BMP of hydroxyapatite surface, can realize that by its induced osteogenesis effect the physiology of artificial joint fixes.
Advantage of the present invention just is to use BMP induced osteogenesis effect, makes synosteosis in area of new bone and the body, realizes that the physiology of artificial joint is fixed, and solves the loosening problem after artificial joint is implanted effectively.
Embodiment
Do the animal contrast experiment with Canis familiaris L., two batches of hip joints have been prepared respectively, a collection of is the Margarita face joint that is not coated with bone morphogenetic protein, a collection of is the Margarita face joint that scribbles bone morphogenetic protein with the inventive method manufacturing, be installed on the Canis familiaris L. hipbone, after 3 months, the Margarita face recess that the dissection discovery does not contain the Margarita face joint of bone morphogenetic protein has only the 40-60% space to be filled by fibrous tissue; And the Margarita face recess that scribbles the joint of bone morphogenetic protein is all filled by the inductive area of new bone of BMP more than 95%.Show by animal experiment, owing to the induced osteogenesis effect of bone morphogenetic protein, can make the Margarita face recess of the quick filling Margarita of area of new bone face hip joint, and interconnect, effectively improve bond strength, prevent the loosening of joint with the body internal skeleton.
Claims (2)
1, a kind of artificial joint that scribbles bone morphogenetic protein, be characterised in that: at artificial joint surface spraying one deck hydroxyapatite, thickness is 50-100 μ m, and then at hydroxyapatite coating layer surface recombination last layer bone morphogenetic protein, thickness is 0.1-1 μ m.
2, the manufacture method of the described artificial joint of a kind of claim 1 is characterized in that: concrete production stage is as follows:
(1) process artificial joint with titanium-base alloy or vitallium,
(2) spraying one deck hydroxyapatite is stand-by on described artificial joint,
(3) add the phosphate buffer of 1-10% (percent by volume) 10mM in the carbamide of 5-7M, the control acid-base value is PH6-8,
(4) then bone morphogenetic protein is dissolved in the above-mentioned urea liquid, addition is the bone morphogenetic protein or 10 of 0.01-0.05mg/ml natural extract
-5-10
-3The bone morphogenetic protein of mg/ml gene recombinaton forms the urea liquid that contains bone morphogenetic protein,
(5) will be coated with the artificial joint of hydroxyapatite surface coating again, put into the above-mentioned urea liquid that contains bone morphogenetic protein, soak 10-14 hour down at 20-30 ℃, every 2 hours slow agitating solution 1-3 minute, then with the artificial joint taking-up,
(6) be the artificial joint temperature of above-mentioned taking-up that 1-10 ℃, concentration are 85% the careful drip washing of alcoholic solution 2 times, the reuse temperature is that 1-10 ℃, concentration are 100% alcoholic solution drip washing one time, airing, aseptic preservation, stand-by, promptly make the artificial joint of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98125674A CN1086591C (en) | 1998-12-21 | 1998-12-21 | Artificial joint coated with bone morphogenesis protein and its making method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98125674A CN1086591C (en) | 1998-12-21 | 1998-12-21 | Artificial joint coated with bone morphogenesis protein and its making method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1220169A true CN1220169A (en) | 1999-06-23 |
| CN1086591C CN1086591C (en) | 2002-06-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98125674A Expired - Fee Related CN1086591C (en) | 1998-12-21 | 1998-12-21 | Artificial joint coated with bone morphogenesis protein and its making method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101732762A (en) * | 2010-01-01 | 2010-06-16 | 东南大学 | Bioactive artificial joint capable of slowly releasing trace element selenium |
| CN101721743B (en) * | 2009-12-24 | 2012-09-05 | 曲彦隆 | Method for strengthening joint stability by using rhBMP-2 release coating on surface of artificial joint |
| CN115068689A (en) * | 2022-06-17 | 2022-09-20 | 北京邦塞科技有限公司 | Bone filling capsular bag and preparation method and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1031040C (en) * | 1989-11-16 | 1996-02-21 | 齐齐哈尔轻工学院 | Biological active coating-Ti alloy man-made bone, joint and its preparation |
| CN2183774Y (en) * | 1994-05-03 | 1994-11-30 | 北京京航生物医学工程公司 | Artificial joint with hydroxy apatite surface |
| CN1081072C (en) * | 1997-11-19 | 2002-03-20 | 华南理工大学 | Method for preparing artificial joint with bio-active gradient coating |
-
1998
- 1998-12-21 CN CN98125674A patent/CN1086591C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101721743B (en) * | 2009-12-24 | 2012-09-05 | 曲彦隆 | Method for strengthening joint stability by using rhBMP-2 release coating on surface of artificial joint |
| CN101732762A (en) * | 2010-01-01 | 2010-06-16 | 东南大学 | Bioactive artificial joint capable of slowly releasing trace element selenium |
| CN101732762B (en) * | 2010-01-01 | 2013-04-03 | 东南大学 | Bioactive artificial joint capable of slowly releasing trace element selenium |
| CN115068689A (en) * | 2022-06-17 | 2022-09-20 | 北京邦塞科技有限公司 | Bone filling capsular bag and preparation method and application thereof |
| CN115068689B (en) * | 2022-06-17 | 2024-01-19 | 北京邦塞科技有限公司 | Bone filling bag, and preparation method and application thereof |
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| Publication number | Publication date |
|---|---|
| CN1086591C (en) | 2002-06-26 |
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