CN1205078A - Liquid treatment method for samples for microscopy - Google Patents
Liquid treatment method for samples for microscopy Download PDFInfo
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- CN1205078A CN1205078A CN 97191264 CN97191264A CN1205078A CN 1205078 A CN1205078 A CN 1205078A CN 97191264 CN97191264 CN 97191264 CN 97191264 A CN97191264 A CN 97191264A CN 1205078 A CN1205078 A CN 1205078A
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Abstract
Liquid treatment method for samples for microscopy. An apparatus for automatically dyeing samples for microscopy, wherein slide glasses (12) supported on a dyeing cage (6) are immersed completely in the cleaning water (11) stored in a liquid storage vessel (2), and thereafter pull up the slide glasses completely from the cleaning water (11). These actions are made repeatedly to promote the replacement of the cleaning water in the clearances (13) between adjacent slide glasses (12) by new cleaning water. Thus, an operation for washing away the chemicals deposited on the slide glasses (12) can be carried out effectively for conducting a sample dyeing treatment.
Description
Technical field
The present invention relates to a kind of will be with the method for the liquid handling of fractographic biological tissue sheet or cell (hereinafter being called sample) in clinic, hospital or various research institute, particularly a kind of in the automatic staining device of the samples for microscopy that utilizes dyeing to handle, flush away is attached to the improvement of the method for soup on the microslide.
Background technology
In clinic and hospital, usually will be from the sample under the affected part excision with microscopic examination to carry out the diagnosis of disease.In order to be easy to microscopic examination, to diagnose the illness, the various automatic staining devices that carry out such dyeing operation are automatically provided at present with the test dyeing that is attached on the microslide.
Fig. 2 shows an example of such automatic staining device, and it is documented in, and Japan is real to open in the flat 5-66543 communique.Have above top in casing 1 is being provided with a row state opening, store a plurality of storage liquid containers 2 having dyeing to handle necessary various soups.Parts in these storage liquid containers 2 are freely to supply with the washing container of rinse water such as tap water, and remaining is the medical liquid container that storage has the necessary various soups of operation that dye.The top of these storage liquid containers 2 is provided with three-dimensional drive unit, by the trave lling girder 3 that can move freely, by moving freely along trave lling girder 3 in horizontal (a direction of Fig. 2) level of casing 1, the mobile post 4 that can move freely and move freely and the cantilever 5 of free lifting constitutes by the vertical direction that moves post 4 along this along vertical (the b direction of Fig. 2) level of casing 1.
As shown in Figure 3, the dyeing cage 6 on this cantilever 5 can freely load and unload.That is, above-mentioned dyeing cage 6 has microslide receiving portion 7 and handle 8, in addition, is provided with joint fastener 9 in the central authorities of handle 8.The front end of above-mentioned cantilever 5 freely engages with joint fastener 9 by inserting the joint fixed orifice 10 that is provided with on the joint fastener 9.So under cantilever 5 and joint fastener 9 engagement states, by falling cantilever 5, dyeing cage 6 just freely is immersed in the above-mentioned storage liquid container 2.
When carrying out the dyeing processing of sample with above-mentioned automatic staining device, at first, dyeing cage 6 is engaged with cantilever 5, dyeing cage 6 contains and sticks the microslide of wanting dyeing sample.Then, drive above-mentioned three-dimensional drive unit, the trave lling girder 3, mobile post 4 and the cantilever 5 that constitute this three-dimensional drive unit are moved according to the signal that comes from not shown controller.So above-mentioned dyeing cage 6 order according to the rules is in official hour is immersed in storage liquid container 2.Carry out such operation repeatedly by number of times, with to test dyeing with regulation.In addition, adopt above-mentioned automatic staining device, handle a plurality of dyeing cages 6 side by side, handle also record to some extent in the special fair 6-100522 communique of Japan with the multiple staining that efficient dyes to more samples well, this is that conventional art is known.
That is, the dyeing cage 6 of accommodating the microslide that posts sample is immersed in official hour in the soup, in when dipping, and the disengaging that engages of cantilever 5 and this dyeing cage 6, and engage with other cages 6 that dye, and other cages 6 that dye are sent in the storage liquid container 2 of regulation.Equally, with the bond-off of cantilever 5, other dyeing cages 6 only are immersed in the soup at the appointed time.A plurality of dyeing cages 6 are carried out above-mentioned such joint concurrently, and if any the dyeing cage 6 that the stipulated time dipping finishes, then cantilever 5 engages with this dyeing cage 6, and it is moved in the next storage liquid container 2.By carrying out such action repeatedly, because can a plurality of dyeing cages 6 of parallel processing, compare with aforesaid common processing, can dye to more samples at short notice.Such multiple staining handle can be in above-mentioned controller storage processing program and realizing in advance.
As above-mentioned to being attached to when sample on the microslide dyes, after proposing in first soup of above-mentioned dyeing cage 6 from the storage liquid container 2 that is stored in regulation, before this dyeing cage 6 being immersed in second soup of being stored in another storage liquid container 2, reach the first accompanying soup on the microslide in this dyeing cage 6 on this dyeing cage 6 of necessary flush away.Reason is, stops the chemical reaction of first soup, prevents perhaps that first soup from sneaking in second soup and deterioration second soup.
Thus, conventional art is shown in Fig. 4 A and Fig. 4 B, and after above-mentioned dyeing cage 6 was proposed from first soup, flush away was somebody's turn to do the first accompanying soup on dyeing cage 6 and this dyeing cage 6 interior microslides 12.That is, the cantilever 5 of hanging dyeing cage 6 moved to the top of the storage liquid container (washing container) 2 of storing rinse water 11 after, above-mentioned cantilever 5 is fallen so that above-mentioned dyeing cage 6 is immersed in the rinse water 11.Then, by this cantilever 5 of lifting slightly, make the displacement up and down between the state of the state of Fig. 4 A and Fig. 4 B of above-mentioned dyeing cage 6, with the first accompanying soup on the microslides 12 in the above-mentioned dyeing cage 6 of flush away and this dyeing cage 6.
Under situation in the past, the dyeing lifting of cage 6 in rinse water 11 carried out in the following cases, and all or part of that is contained in promptly that microslide 12 surfaces in this dyeing cage 6 go up accompanying sample is immersed in the above-mentioned rinse water 11.Thus, enter in the rinse water 11 in the gap 13 between adjacent microslide 12 lip-deep samples, existing old rinse water is difficult to replace with new rinse water in the bottom in these each gaps 13.As a result, on above-mentioned each microslide 12 in accompanying first soup, be difficult to flush away with first soup accompanying on the mutually opposed part in the bottom in these each gaps 13.
Therefore, washing operation required time in the past is long, promptly needs repeatedly lifting, could the first above-mentioned soup of flush away.Therefore make dyeing operation required time elongated.And, during a plurality of dyeing of parallel processing cage 6,, can not carry out the processing of other dyeing cages 6 at the same time because of washing operation uses the time of cantilever 5 elongated.
The objective of the invention is to solve the above problems, provide a kind of can be than accompanying method for treating liquids soup, samples for microscopy on the flush away microslide more effectively in the past.
Disclosure of an invention
A kind of method for treating liquids provided by the present invention is, cage is impregnated in the liquid in a plurality of storage liquid containers successively to handle sample, wherein posting will be as a plurality of microslides of samples for microscopy sample to exist the gap between adjacent microslide, the upright mode of above-below direction is contained in the described cage, this method comprises, the last ora terminalis that described cage is dropped to microslide relatively is arranged in till the subsurface plane of storage liquid container to be immersed in the step of liquid, with then, described cage is risen to step till the plane more than the plane on the liquid level that ora terminalis under the sample on the slide surface is positioned at the storage liquid container relatively.Usually, carry out aforesaid step repeatedly.
The present invention also provides a kind of method for treating liquids to be, cage is impregnated into successively in the liquid in a plurality of storage liquid containers that comprise medical liquid container and soda liquor container to handle sample, wherein posting will be as a plurality of microslides of samples for microscopy sample to exist the gap between adjacent microslide, the upright mode of above-below direction is contained in the described cage, this method comprises, described cage is immersed in the soup in the described medical liquid container, handle the step of sample on the microslide with soup, with then, with drop to relatively in the cleaning fluid of described cage in described soda liquor container microslide on ora terminalis be arranged in and clean till the subsurface plane to be immersed in the step of cleaning fluid, afterwards, described cage is risen to relatively step till ora terminalis under the sample on the slide surface is positioned at plane more than the plane of cleaning on the liquid level.Usually, be impregnated into the step and the step that rises on the described cleaning liquid level of described cleaning fluid repeatedly.
The abovementioned steps of carrying out can be carried out in same soda liquor container repeatedly, also can carry out in different a plurality of soda liquor containers.The step of handling with aforementioned soup is typical staining procedure.In addition, aforementioned cleaning fluid typically is water.
In the present invention, by adopting above-mentioned steps, in adjacent microslide gap the major part of cleaning fluid that exist, that contain the soup of wanting flush away by the gap towards below discharge.So under microslide is immersed in state in the cleaning fluid once more, can sneak into soup or this soup mixed volume cleaning fluid seldom, new of wanting flush away and enter in the above-mentioned gap.As a result, can carry out effectively in the flush away microslide part, with the opposed part in above-mentioned gap on the operation of accompanying soup.
The simple declaration of drawing
Figure 1A and Figure 1B schematically illustrate the longitudinal diagram by accompanying soup state on method for treating liquids of the present invention, the usefulness cleaning fluid flush away microslide.
Fig. 2 illustrates the skeleton view of implementing automatic staining device one example method for treating liquids of the present invention, samples for microscopy.
Fig. 3 illustrates the skeleton view of dyeing cage one example.
Fig. 4 A and Fig. 4 B schematically illustrate the longitudinal diagram by accompanying soup state on previous methods, the usefulness rinse water flush away microslide.
The optimised form that carries out an invention
Fig. 1 illustrates one embodiment of the present of invention.The feature of method for treating liquids of the present invention is, in the automatic staining device of Fig. 2 samples for microscopy illustrated, known forms, from storage have the dyeing cage 6 that promotes the medical liquid container of regulation soup enter store in the medical liquid container that other soups are arranged before, effectively on the flush away dyeing cage 6 and be contained in accompanying afore mentioned rules soup on the microslide 12 in the dyeing cage 6.The structure of the automatic staining device of samples for microscopy and effect are not limited to the structure in the past of earlier figures 2, therefore, and for the purpose of omitting explanation, below, characteristic of the present invention only is described.
From storage have the dyeing cage 6 that promotes the medical liquid container of afore mentioned rules soup enter store in the medical liquid container that other soups are arranged before, for on the flush away dyeing cage 6 and be contained in the soup of afore mentioned rules accompanying on the microslide 12 in the dyeing cage 6, make the cantilever 5 of hanging this dyeing cage 6 move to top as the storage liquid container 2 of the washing container of storage rinse water 11.Then, fall cantilever 5, make the dyeing cage of hanging by cantilever 56 shown in Figure 1A, be immersed in the rinse water 11 that above-mentioned storage liquid container 2 stored, till the last ora terminalis of the microslide 12 in being contained in this dyeing cage 6 is positioned under the surface level.Afterwards, cantilever 5 is risen, shown in Figure 1B, the following ora terminalis of above-mentioned each microslide 12 is positioned on the surface level.At this moment, the following ora terminalis part of microslide 12 is under the surface level, and the sample on the slide surface self is on the surface level fully and also is fine.Then, by falling cantilever 5, carry out the state of Figure 1A and the state of Figure 1B repeatedly with the number of times of stipulating.
Adopt above-mentioned structure, when carrying out the method for treating liquids of samples for microscopy of the present invention, shown in Figure 1B, be positioned under the state more than the plane on the surface level cantilever 5 being risen to the following ora terminalis that is contained in sample on microslide 12 surfaces of dyeing in the cage 6, adjacent microslide 12 to each other or and slide surface on the major part of rinse water that exist in the gap 13 between the adjacent slide surface of sample, that sneaked into the soup of wanting flush away under action of gravity, below being discharged to from this gap 13.For the rinse water that promotes to sneak into soup from this gap 13 discharge downwards, also can vibrate dyeing cage 6 and its inner microslide 12.Then, under the state that microslide 12 is immersed in once more in the rinse water 11, less, the new rinse water of soup mixed volume of flush away to flow in the gap 13.As a result, effectively in the part of flush away microslide 12, with gap 13 mutually opposed parts on accompanying soup.
In addition, in illustrated example, dyeing cage 6 is immersed in back in the rinse water 11, the operation of cantilever 5 risings is carried out at same storage liquid container 2.Corresponding with it, the storage liquid container 2 of storing rinse water 11 as the washing container can adopt a plurality of, after being immersed in above-mentioned dyeing cage 6 in first rinse water 11 of storing in the liquid container, from this storage liquid container 2, propose dyeing cage 6, and the cage 6 that should dye is immersed in second, third ... in the rinse water 11 in the storage liquid container.In addition, can also can make the upper-lower position of dyeing cage 6 motionless dyeing cage 6 and the 2 relative liftings of storage liquid container, and lifting storage liquid container 2.But, preferably, making 6 liftings of dyeing cage, the institute of lifting like this energy requirement is less, and the liquid in the storage liquid container 2 is difficult for overflowing.
The method for treating liquids of samples for microscopy of the present invention can shorten the dyeing processing and wait sample to handle the required time owing to above-mentioned structure function, can carry out the operation of soup accompanying on the flush away microslide effectively.In addition, at the same time during a plurality of dyeing cage of parallel processing, use the time of the cantilever that clean-out operation uses to be shortened, thereby be easy to carry out the processing of other dyeing cages.
The method for treating liquids of the application of the invention, effectively the result of flush away soup is, it is typical uneven dyeing phenomenon that no sample is handled irregular, has improved dyeability.And, when dyeing, can be attached to the remaining dyeing liquor on microslide and the dyeing cage at short notice with a spot of cleaning fluid flush away.In addition, during cleaning, and when cleaning when flooding in the extroversion storage liquid container, can reduce the stirring action frequency of microslide cage, when a plurality of microslide cage of parallel processing, this effect is more remarkable.Although in the past when being immersed in the microslide cage in soda liquor container and the medical liquid container, be stirring and washing liquid and soup continuously, reduce that to stir number of times also be very favorable.In addition, the result who adopts method for treating liquids of the present invention can replace the liquid between microslide effectively is, can reduce the spacing between microslide, reduces the configured length of microslide.
The possibility of utilizing on the industry
Method for treating liquids of the present invention is not limited to biological tissue's sheet or the such sample of cell are attached to On the slide surface and to by washing behind the glass slide dyeing that supports of dyeing cage, also can In the storage liquid container situation beyond the washing container, the liquid in the displacement slide gap.
Claims (8)
1, a kind of method for treating liquids of samples for microscopy, be that cage is impregnated in the liquid in a plurality of storage liquid containers successively to handle sample, wherein post and to be contained in the described cage to have gap, the upright mode of above-below direction between adjacent microslide as a plurality of microslides of the sample of samples for microscopy, this method comprises
The last ora terminalis that described cage is dropped to microslide relatively is arranged in till the subsurface plane of storage liquid container to be immersed in the step of liquid, then,
Described cage is risen to step till the plane more than the plane on the liquid level that ora terminalis under the sample on the slide surface is positioned at the storage liquid container relatively.
2, according to the described method for treating liquids of claim 1, it is characterized in that, carry out described step repeatedly.
3, a kind of method for treating liquids of samples for microscopy, cage is impregnated into successively in the liquid in a plurality of storage liquid containers that comprise medical liquid container and soda liquor container to handle sample, wherein post and to be contained in the described cage to have gap, the upright mode of above-below direction between adjacent microslide as a plurality of microslides of the sample of samples for microscopy, this method comprises
Described cage is immersed in the soup in the described medical liquid container, handles the step of sample on the microslide with soup, then,
With drop to relatively in the cleaning fluid of described cage in described soda liquor container microslide on ora terminalis be arranged in and clean till the subsurface plane to be immersed in the step of cleaning fluid, afterwards,
Described cage is risen to step till ora terminalis under the sample on the slide surface is positioned at plane more than the plane of cleaning on the liquid level relatively.
4, according to the described method for treating liquids of claim 3, it is characterized in that, be impregnated into the step and the step that rises on described cleaning liquid level of described cleaning fluid repeatedly.
According to the described method for treating liquids of claim 4, it is characterized in that 5, the described step of carrying out is repeatedly carried out with same soda liquor container.
According to the described method for treating liquids of claim 4, it is characterized in that 6, the described step of carrying out is repeatedly carried out with different a plurality of soda liquor containers.
According to the described method for treating liquids of claim 3, it is characterized in that 7, the step of handling with described soup is a staining procedure.
According to the described method for treating liquids of claim 3, it is characterized in that 8, described cleaning fluid is a water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97191264 CN1205078A (en) | 1996-09-18 | 1997-09-18 | Liquid treatment method for samples for microscopy |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP246602/96 | 1996-09-18 | ||
| CN 97191264 CN1205078A (en) | 1996-09-18 | 1997-09-18 | Liquid treatment method for samples for microscopy |
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| Publication Number | Publication Date |
|---|---|
| CN1205078A true CN1205078A (en) | 1999-01-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 97191264 Pending CN1205078A (en) | 1996-09-18 | 1997-09-18 | Liquid treatment method for samples for microscopy |
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| CN (1) | CN1205078A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102251024A (en) * | 2011-04-21 | 2011-11-23 | 中国海洋大学 | Chromosome specimen preparation liquid for laser microdissection and application thereof |
| WO2013152697A1 (en) * | 2012-04-10 | 2013-10-17 | 深圳迈瑞生物医疗电子股份有限公司 | Method and device for cleaning applicator paddle of paddle dyeing machine |
| CN103487308A (en) * | 2013-09-27 | 2014-01-01 | 上海海洋大学 | Staining jar cover |
| CN114323883A (en) * | 2021-12-31 | 2022-04-12 | 迈克医疗电子有限公司 | Slide glass processing device |
| CN114761128A (en) * | 2019-11-20 | 2022-07-15 | 三菱重工业株式会社 | Catalyst cleaning method, catalyst cleaning device, and program |
-
1997
- 1997-09-18 CN CN 97191264 patent/CN1205078A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102251024A (en) * | 2011-04-21 | 2011-11-23 | 中国海洋大学 | Chromosome specimen preparation liquid for laser microdissection and application thereof |
| CN102251024B (en) * | 2011-04-21 | 2013-01-09 | 中国海洋大学 | Chromosome specimen preparation liquid for laser microdissection and application thereof |
| WO2013152697A1 (en) * | 2012-04-10 | 2013-10-17 | 深圳迈瑞生物医疗电子股份有限公司 | Method and device for cleaning applicator paddle of paddle dyeing machine |
| CN103487308A (en) * | 2013-09-27 | 2014-01-01 | 上海海洋大学 | Staining jar cover |
| CN114761128A (en) * | 2019-11-20 | 2022-07-15 | 三菱重工业株式会社 | Catalyst cleaning method, catalyst cleaning device, and program |
| CN114323883A (en) * | 2021-12-31 | 2022-04-12 | 迈克医疗电子有限公司 | Slide glass processing device |
| CN114323883B (en) * | 2021-12-31 | 2023-11-14 | 迈克医疗电子有限公司 | Slide processing apparatus |
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