CN1204393C - Inductive coupling plasma detection microcurent control chip - Google Patents
Inductive coupling plasma detection microcurent control chip Download PDFInfo
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- CN1204393C CN1204393C CN 02130721 CN02130721A CN1204393C CN 1204393 C CN1204393 C CN 1204393C CN 02130721 CN02130721 CN 02130721 CN 02130721 A CN02130721 A CN 02130721A CN 1204393 C CN1204393 C CN 1204393C
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- microcurent
- coupling plasma
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Abstract
The present invention relates to a micro current control chip for detecting an inductive coupling plasma, which is characterized in that the chip is provided with a detection interface for the inductive coupling plasma; the interface is composed of an atomizing chamber placed in the position of a detection opening of the chip and an air loading pipe; the detection opening is of a through hole structure; a detection electrode is introduced from the interior of the chip to the exterior of the chip; one surface of the detection opening on the chip is connected with the atomizing chamber in a sealing mode, and the other surface is connected with the air loading pipe in the sealing mode. The present invention can be connected with an inductively coupled plasma spectrum/mass spectrometer to be used to realize analysis detection to element forms.
Description
Technical field:
The present invention relates to the micro-total analysis technology, a kind of micro-fluidic chip that carries out check and analysis with inductively coupled plasma is provided especially.
Background technology:
Micro-total analysis system (Micro Total Analysis System, μ-TAS) is a frontier interdisciplinary, its final purpose is by the microminiaturization of chemical analysis apparatus and integrated, to greatest extent the function of assay laboratory is transferred to (as all kinds of chips) in the portable analytical equipment, realize " microminiaturization " of assay laboratory, therefore, micro-total analysis system also generically is called " chip lab " (Lab-On-A-Chip).Can be divided into two big classes again according to chip structure and working mechanism: micro-array chip (Microarray Chip), be called " biochip " again, and micro-fluidic chip (MicrofluidicChip), the former development is quite ripe, degree of depth industrialization abroad; The latter is mainly based on analytical chemistry and biological chemistry, utilize micro electronmechanical process technology (MEMS), process the microchannel network of 10-100 micron at silicon, glass, quartz, frosting, be driving force mainly with electroosmotic flow and electrophoresis stream, by changing driving voltage, control flow direction and the speed of fluid in the network of microchannel, thus realize to target analytes sampling, dilute, add steps such as reagent, enrichment, extraction, mixing, reaction, separation, detection.In other words, micro-fluidic chip can be regarded as that of process is analyzed in sample introduction, sample preparation, separation, detection etc. is integrated, so detection technique is a big problem that must emphasis in the micro-fluidic chip development will solve obviously.The most frequently used detecting device is fluorescence, absorption and electrochemical detector at present.
Because the different shape of element has different chemistry and biological behavior, so the morphological research of element causes people's attention gradually in fields such as environment, biology, geology and pharmacy.Inductively coupled plasma (ICP) is as a kind of efficient atomic emissions light source, in geochemistry, life science and environmental science, obtained aspect the mensuration of various elements using widely with spectrum/mass spectrometric coupling, but can't carry out Elemental Speciation Analysis.,, behind ICP spectrum/mass spectrometry, the Elemental Speciation Analysis of various samples is appeared in the newspapers as a kind of separation means with Capillary Electrophoresis, document 1B ' Hymer C, Day AJ, Caruso JA, Applied Spectroscopy, 2000,54 (7): 1040-1046, document 2Stewart II, Olesik JW, J.Chromatogr., 2000,872:227-246, document 3Michalke B, J.Anal.At.Spectrom., 1999,14:1297-1302.But in micro-fluidic chip, use ICP spectrum/mass spectrum not appear in the newspapers so far.
The invention technology contents:
The purpose of this invention is to provide a kind of inductive coupling plasma detection microcurent control chip, its can with inductively coupled plasma-spectrum/mass spectrometer coupling, realize analyzing and testing to element morphology.
The invention provides a kind of inductive coupling plasma detection microcurent control chip, it is characterized in that this chip has inductively coupled plasma and detects interface.
In the inductive coupling plasma detection microcurent control chip of the present invention, described interface is made of spray chamber that is positioned at chip detection mouth place and carrier gas pipe, and detecting mouth is through-hole structure, and detecting electrode is drawn out to outside the chip from chip, detect the one side sealing-in spray chamber of mouth on the chip, another side sealing-in carrier gas pipe.
In the inductive coupling plasma detection microcurent control chip of the present invention, the material of spray chamber can be quartz or glass, and the material of carrier gas pipe can be PTFE or various plastics, and chip material is quartz, glass or PMMA, PDMS polymkeric substance.
The present invention combines chip design and Interface design together, has solved the required requirement of guaranteeing current return of capillary electrophoresis chip, simultaneously can be simply, easily realizes being connected with instrument such as ICP spectrum/mass spectrum.Can carry out morphological analysis to the element in the samples such as environment, life science, and required sample size is few, it is rapid to analyze.
Description of drawings:
Fig. 1 inductive coupling plasma detection microcurent control chip floor layout;
Fig. 2 inductively coupled plasma detects the partial enlarged drawing that the micro flow chip electrode connects;
The Interface design of Fig. 3 inductive coupling plasma detection microcurent control chip and ICP.
Fig. 4 is the separation spectrogram of embodiment 1 metallothionein;
Fig. 5 is the separation spectrogram of the different shape of embodiment 2Cr.
Embodiment:
Fig. 1 is the inductive coupling plasma detection microcurent control chip design drawing, and the microchannel c that chip edge is arrived in its below is used for the installation of electrode.Fig. 2 is the electrode connection diagram that detects mouth 4 and microchannel c.When chip connects with ICP, the design drawing of interface is as shown in Figure 3: in Fig. 3, atomization gas is from the inductive coupling plasma detection microcurent control chip below, blow to the chip top by detecting mouth 4, the sample solution that will move out from the microchannel blows in the glass atomization chamber and atomizes, and enters into ICP afterwards again.The present invention utilizes the passage of inductive coupling plasma detection microcurent control chip side to insert electrode, forms current return.At the sample export end, atomization gas feeds from the inductive coupling plasma detection microcurent control chip below, utilizes high velocity carrier gas that sample solution is atomized, and is brought in the plasma.
Embodiment 1:
In chip as shown in Figure 1,1 is buffer pool, and 2 is sample cell, and 3 is waste liquid pool, and 4 for detecting mouthful (being the passage of carrier gas).With concentration is that the metallothionein of 1mg/ml is placed in the sample cell 2, puts into pH 4.0 in buffer pool 1, and concentration is the sodium acetate solution of 14mmol/L, afterwards, makes respectively among the passage b to be full of sample solution, is full of damping fluid among the split tunnel a.Between 2 and 3, apply the voltage of 1kV, carry out the sample introduction process.Then 1 and c between apply the voltage of 1.2kV, the flow velocity of adjusting atomization gas simultaneously is 1.6L/min, begins to separate.By resulting spectrogram as can be known, two kinds of form: MT1 of metallothionein (MT) have obtained separating with MT2, and are respond well.
Embodiment 2:
In chip as shown in Figure 1,1 is buffer pool, and 2 is sample cell, and 3 is waste liquid pool, and 4 for detecting mouthful (being the passage of carrier gas).In sample cell 2, put into the Cr3+ that contains 50 μ g/ml and the Cr2O72-sample solution of 50 μ g/l, put into the sodium acetate solution of 20mmol/L in the buffer pool 1, make respectively among the passage b to be full of sample solution, be full of damping fluid among the split tunnel a.Between 2 and 3, apply the voltage of 1kV, carry out the sample introduction process.Then 1 and c between apply the voltage of 1.2kV, the flow velocity of atomization gas is 1.6L/min, begins to separate.By resulting spectrogram as can be known, the Cr of two kinds of different shapes has obtained baseline separation substantially.
Claims (3)
1, a kind of inductive coupling plasma detection microcurent control chip is characterized in that this chip has inductively coupled plasma and detects interface; Described interface is made of spray chamber that is positioned at chip detection mouth place and carrier gas pipe, and detecting mouth is through-hole structure, and detecting electrode is drawn out to from chip outside the chip, detects the one side sealing-in spray chamber of mouth on the chip, another side sealing-in carrier gas pipe.
2, according to the described inductive coupling plasma detection microcurent control chip of claim 1, it is characterized in that: the material of described spray chamber is quartz or glass, and the carrier gas tube material is teflon or various plastic tube.
3, according to claim 1 or 2 described inductive coupling plasma detection microcurent control chips, it is characterized in that: described chip material is quartz, glass, polymethylmethacrylate or dimethyl silicone polymer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02130721 CN1204393C (en) | 2002-09-18 | 2002-09-18 | Inductive coupling plasma detection microcurent control chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02130721 CN1204393C (en) | 2002-09-18 | 2002-09-18 | Inductive coupling plasma detection microcurent control chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1484016A CN1484016A (en) | 2004-03-24 |
| CN1204393C true CN1204393C (en) | 2005-06-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 02130721 Expired - Fee Related CN1204393C (en) | 2002-09-18 | 2002-09-18 | Inductive coupling plasma detection microcurent control chip |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101551357B (en) * | 2009-02-26 | 2013-02-06 | 中国兵器工业集团第五三研究所 | ICP-MS measuring method of trace metal impurities in high purity lead |
| CN106442476A (en) * | 2016-11-09 | 2017-02-22 | 中国科学院广州地球化学研究所 | Inductively coupled plasma light source sharing light-spectrum and mass-spectrum measuring device and method |
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- 2002-09-18 CN CN 02130721 patent/CN1204393C/en not_active Expired - Fee Related
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| CN1484016A (en) | 2004-03-24 |
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