CN120092083A - 包含Cas蛋白和细菌毒素的融合蛋白及其用途 - Google Patents

包含Cas蛋白和细菌毒素的融合蛋白及其用途 Download PDF

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Publication number
CN120092083A
CN120092083A CN202380073912.9A CN202380073912A CN120092083A CN 120092083 A CN120092083 A CN 120092083A CN 202380073912 A CN202380073912 A CN 202380073912A CN 120092083 A CN120092083 A CN 120092083A
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China
Prior art keywords
fusion protein
ssda
protein
toxin
cas
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Pending
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CN202380073912.9A
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English (en)
Chinese (zh)
Inventor
金容涉
权志妍
金大植
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Sungkyunkwan University School Industry Cooperation
Asan Foundation
University of Ulsan
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Sungkyunkwan University School Industry Cooperation
Asan Foundation
University of Ulsan
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Publication of CN120092083A publication Critical patent/CN120092083A/zh
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
    • C—CHEMISTRY; METALLURGY
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    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04001—Cytosine deaminase (3.5.4.1)
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    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07K2319/00—Fusion polypeptide
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202380073912.9A 2022-10-19 2023-10-19 包含Cas蛋白和细菌毒素的融合蛋白及其用途 Pending CN120092083A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20220134734 2022-10-19
KR10-2022-0134734 2022-10-19
PCT/KR2023/016230 WO2024085674A1 (ko) 2022-10-19 2023-10-19 Cas 단백질 및 박테리아 톡신을 포함하는 융합 단백질 및 이의 용도

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CN120092083A true CN120092083A (zh) 2025-06-03

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CN202380073912.9A Pending CN120092083A (zh) 2022-10-19 2023-10-19 包含Cas蛋白和细菌毒素的融合蛋白及其用途

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Country Link
US (1) US20260139237A1 (enExample)
EP (1) EP4606894A4 (enExample)
JP (1) JP2025535373A (enExample)
KR (1) KR20240055677A (enExample)
CN (1) CN120092083A (enExample)
WO (1) WO2024085674A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20250148471A (ko) * 2024-04-02 2025-10-14 재단법인 아산사회복지재단 핵산 결합 단백질 및 SsdA를 포함하는 융합단백질 및 이의 용도

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017070632A2 (en) * 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
CN109312386B (zh) * 2016-06-15 2022-10-25 株式会社图尔金 使用中靶靶标和脱靶靶标的多重靶标系统筛选靶特异性核酸酶的方法及其用途
CN112135640A (zh) * 2018-04-30 2020-12-25 俄勒冈健康科学大学 基因疗法的方法
GB201913060D0 (en) * 2019-09-10 2019-10-23 Innes John Centre Methods of increasing biotic stress resistance in plants
WO2022212584A1 (en) * 2021-04-01 2022-10-06 University Of Washington Bacterial dna cytosine deaminases for mapping dna methylation sites

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KR20240055677A (ko) 2024-04-29
US20260139237A1 (en) 2026-05-21
EP4606894A1 (en) 2025-08-27
EP4606894A4 (en) 2026-08-19
WO2024085674A1 (ko) 2024-04-25
JP2025535373A (ja) 2025-10-24

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