CN119907855A - 用于长dna分子测序的溶液中位置协同条形码化的方法 - Google Patents
用于长dna分子测序的溶液中位置协同条形码化的方法 Download PDFInfo
- Publication number
- CN119907855A CN119907855A CN202380055820.8A CN202380055820A CN119907855A CN 119907855 A CN119907855 A CN 119907855A CN 202380055820 A CN202380055820 A CN 202380055820A CN 119907855 A CN119907855 A CN 119907855A
- Authority
- CN
- China
- Prior art keywords
- sequence
- primer
- adapter
- fragments
- adaptor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263369346P | 2022-07-25 | 2022-07-25 | |
| US63/369,346 | 2022-07-25 | ||
| PCT/CN2023/108314 WO2024022207A1 (en) | 2022-07-25 | 2023-07-20 | Methods of in-solution positional co-barcoding for sequencing long dna molecules |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN119907855A true CN119907855A (zh) | 2025-04-29 |
Family
ID=89705400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202380055820.8A Pending CN119907855A (zh) | 2022-07-25 | 2023-07-20 | 用于长dna分子测序的溶液中位置协同条形码化的方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20260009071A1 (https=) |
| EP (1) | EP4562143A1 (https=) |
| JP (1) | JP2025526393A (https=) |
| CN (1) | CN119907855A (https=) |
| AU (1) | AU2023316513A1 (https=) |
| CA (1) | CA3262390A1 (https=) |
| WO (1) | WO2024022207A1 (https=) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010534474A (ja) * | 2007-07-26 | 2010-11-11 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | 分子冗長配列決定 |
| US20120003657A1 (en) * | 2010-07-02 | 2012-01-05 | Samuel Myllykangas | Targeted sequencing library preparation by genomic dna circularization |
| CA3006792A1 (en) * | 2015-12-08 | 2017-06-15 | Twinstrand Biosciences, Inc. | Improved adapters, methods, and compositions for duplex sequencing |
| GB2581599B8 (en) * | 2017-08-10 | 2023-09-20 | Element Biosciences Inc | Tagging nucleic acid molecules from single cells for phased sequencing |
| EP4245861B1 (en) * | 2018-05-08 | 2025-02-26 | MGI Tech Co., Ltd. | Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly |
-
2023
- 2023-07-20 WO PCT/CN2023/108314 patent/WO2024022207A1/en not_active Ceased
- 2023-07-20 CA CA3262390A patent/CA3262390A1/en active Pending
- 2023-07-20 CN CN202380055820.8A patent/CN119907855A/zh active Pending
- 2023-07-20 EP EP23845418.5A patent/EP4562143A1/en active Pending
- 2023-07-20 US US18/998,483 patent/US20260009071A1/en active Pending
- 2023-07-20 AU AU2023316513A patent/AU2023316513A1/en active Pending
- 2023-07-20 JP JP2025504308A patent/JP2025526393A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20260009071A1 (en) | 2026-01-08 |
| AU2023316513A1 (en) | 2025-02-13 |
| JP2025526393A (ja) | 2025-08-13 |
| EP4562143A1 (en) | 2025-06-04 |
| CA3262390A1 (en) | 2024-02-01 |
| WO2024022207A1 (en) | 2024-02-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
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