CA3262390A1 - POSITIONAL JOINT BARCODE SOLUTION METHODS FOR SEQUENCING LONG DNA MOLECULES - Google Patents

POSITIONAL JOINT BARCODE SOLUTION METHODS FOR SEQUENCING LONG DNA MOLECULES

Info

Publication number
CA3262390A1
CA3262390A1 CA3262390A CA3262390A CA3262390A1 CA 3262390 A1 CA3262390 A1 CA 3262390A1 CA 3262390 A CA3262390 A CA 3262390A CA 3262390 A CA3262390 A CA 3262390A CA 3262390 A1 CA3262390 A1 CA 3262390A1
Authority
CA
Canada
Prior art keywords
barcoding
positional
methods
solution
dna molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3262390A
Other languages
English (en)
French (fr)
Inventor
Brock A. Peters
Radoje T. Drmanac
Andrei Alexeev
Original Assignee
MGI Tech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MGI Tech Co Ltd filed Critical MGI Tech Co Ltd
Publication of CA3262390A1 publication Critical patent/CA3262390A1/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3262390A 2022-07-25 2023-07-20 POSITIONAL JOINT BARCODE SOLUTION METHODS FOR SEQUENCING LONG DNA MOLECULES Pending CA3262390A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202263369346P 2022-07-25 2022-07-25
US63/369,346 2022-07-25
PCT/CN2023/108314 WO2024022207A1 (en) 2022-07-25 2023-07-20 Methods of in-solution positional co-barcoding for sequencing long dna molecules

Publications (1)

Publication Number Publication Date
CA3262390A1 true CA3262390A1 (en) 2024-02-01

Family

ID=89705400

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3262390A Pending CA3262390A1 (en) 2022-07-25 2023-07-20 POSITIONAL JOINT BARCODE SOLUTION METHODS FOR SEQUENCING LONG DNA MOLECULES

Country Status (7)

Country Link
US (1) US20260009071A1 (https=)
EP (1) EP4562143A1 (https=)
JP (1) JP2025526393A (https=)
CN (1) CN119907855A (https=)
AU (1) AU2023316513A1 (https=)
CA (1) CA3262390A1 (https=)
WO (1) WO2024022207A1 (https=)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010534474A (ja) * 2007-07-26 2010-11-11 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 分子冗長配列決定
US20120003657A1 (en) * 2010-07-02 2012-01-05 Samuel Myllykangas Targeted sequencing library preparation by genomic dna circularization
CA3006792A1 (en) * 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
GB2581599B8 (en) * 2017-08-10 2023-09-20 Element Biosciences Inc Tagging nucleic acid molecules from single cells for phased sequencing
EP4245861B1 (en) * 2018-05-08 2025-02-26 MGI Tech Co., Ltd. Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly

Also Published As

Publication number Publication date
US20260009071A1 (en) 2026-01-08
AU2023316513A1 (en) 2025-02-13
JP2025526393A (ja) 2025-08-13
CN119907855A (zh) 2025-04-29
EP4562143A1 (en) 2025-06-04
WO2024022207A1 (en) 2024-02-01

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