CN119698464A - 从灵长类的胚胎卵巢细胞诱导卵泡的体外培养法 - Google Patents

从灵长类的胚胎卵巢细胞诱导卵泡的体外培养法 Download PDF

Info

Publication number
CN119698464A
CN119698464A CN202380056350.7A CN202380056350A CN119698464A CN 119698464 A CN119698464 A CN 119698464A CN 202380056350 A CN202380056350 A CN 202380056350A CN 119698464 A CN119698464 A CN 119698464A
Authority
CN
China
Prior art keywords
cells
cell
ovaries
culture
germ
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202380056350.7A
Other languages
English (en)
Chinese (zh)
Inventor
斋藤通纪
大田浩
水田贤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyoto University NUC
Original Assignee
Kyoto University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyoto University NUC filed Critical Kyoto University NUC
Publication of CN119698464A publication Critical patent/CN119698464A/zh
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0608Germ cells
    • C12N5/0609Oocytes, oogonia
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/32Amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/38Hormones with nuclear receptors
    • C12N2501/385Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/38Hormones with nuclear receptors
    • C12N2501/39Steroid hormones
    • C12N2501/392Sexual steroids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202380056350.7A 2022-07-25 2023-07-24 从灵长类的胚胎卵巢细胞诱导卵泡的体外培养法 Pending CN119698464A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022117665 2022-07-25
JP2022-117665 2022-07-25
PCT/JP2023/027054 WO2024024742A1 (ja) 2022-07-25 2023-07-24 霊長類の胎児卵巣細胞から卵胞を誘導する体外培養法

Publications (1)

Publication Number Publication Date
CN119698464A true CN119698464A (zh) 2025-03-25

Family

ID=89706644

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380056350.7A Pending CN119698464A (zh) 2022-07-25 2023-07-24 从灵长类的胚胎卵巢细胞诱导卵泡的体外培养法

Country Status (4)

Country Link
EP (1) EP4563697A4 (https=)
JP (1) JPWO2024024742A1 (https=)
CN (1) CN119698464A (https=)
WO (1) WO2024024742A1 (https=)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2277278A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
EP1215280A1 (en) * 2000-12-13 2002-06-19 Vrije Universiteit Brussel Method for in vitro culture of ovarian follicles
WO2004035766A2 (en) * 2002-10-15 2004-04-29 Novo Nordisk A/S In vitro synchronisation of nuclear and cytoplasmatic maturation of oocytes involving addition and removal of phosphodiesterase type 3 inhibitor
BRPI0418732A (pt) 2004-04-10 2007-09-11 Henkel Kgaa dispositivo para enrolar cabelos
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
US7661738B2 (en) 2006-11-28 2010-02-16 Veritainer Corporation Radiation detection unit for mounting a radiation sensor to a container crane
EP2626416A3 (en) 2007-04-07 2013-12-18 The Whitehead Institute for Biomedical Research Reprogramming of somatic cells
CN101802172A (zh) 2007-05-30 2010-08-11 通用医疗公司 由体细胞产生多能细胞的方法
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
JP5234535B2 (ja) * 2007-06-25 2013-07-10 株式会社ニデック 哺乳動物未成熟卵子の体外成熟培養用添加剤及びそれを用いた成熟卵子の作出方法
WO2017002888A1 (ja) 2015-06-29 2017-01-05 国立大学法人京都大学 多能性幹細胞から生殖細胞への分化誘導方法
US20240271088A1 (en) 2020-08-18 2024-08-15 Kyoto University Method for maintaining and amplifying human primordial germ cells / human primordial germ cell-like cells
JP7649961B2 (ja) 2021-02-01 2025-03-24 パナソニックIpマネジメント株式会社 機器制御システム

Also Published As

Publication number Publication date
EP4563697A4 (en) 2025-11-12
EP4563697A1 (en) 2025-06-04
WO2024024742A1 (ja) 2024-02-01
JPWO2024024742A1 (https=) 2024-02-01

Similar Documents

Publication Publication Date Title
AU2022275469B2 (en) Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells
US12241083B2 (en) Macs-based purification of stem cell-derived retinal pigment epithelium
JP7088496B2 (ja) 網膜組織の製造方法
JP6995314B2 (ja) 網膜組織の製造法
EP3365428B1 (en) Methods of generating human inner ear sensory epithelia and sensory neurons
US10100283B2 (en) Efficient chondrocyte induction method
WO2007002210A2 (en) Embryonic stem cell culture compositions and methods of use thereof
WO2015125926A1 (ja) 栄養膜幹細胞の樹立及び維持方法
JP2022538066A (ja) 網膜色素上皮細胞の自動化生産方法
CN119698464A (zh) 从灵长类的胚胎卵巢细胞诱导卵泡的体外培养法
CN106085951B (zh) 一种建立可持续传代的树鼩精原干细胞细胞系的方法
Hosain et al. Validation of pluripotency of human induced pluripotent stem cells
US20250340836A1 (en) Methods for production of ipscs
HK40109085A (en) Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells
HK40074568A (en) Macs-based purification of stem cell-derived retinal pigment epithelium
JP2024057510A (ja) 紐状構造物の製造方法
CN121712884A (zh) 卵原细胞或前精原细胞的制造方法
Rajala Development of Human Stem Cell Culture Conditions for Clinical Cell Therapy
OJALA Establishing and optimizing feeder cell-free culture methods for human

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination