CN119563023A - 用于crispr指导rna的保护性寡核苷酸 - Google Patents

用于crispr指导rna的保护性寡核苷酸 Download PDF

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Publication number
CN119563023A
CN119563023A CN202380043499.1A CN202380043499A CN119563023A CN 119563023 A CN119563023 A CN 119563023A CN 202380043499 A CN202380043499 A CN 202380043499A CN 119563023 A CN119563023 A CN 119563023A
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Prior art keywords
oligonucleotide
protective
crrna
moiety
double stranded
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CN202380043499.1A
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Chinese (zh)
Inventor
张晗
埃里克·约瑟夫·桑特海默
乔纳森·肯尼斯·沃茨
阿纳斯塔西娅·赫沃罗娃
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University of Massachusetts Amherst
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University of Massachusetts Amherst
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Publication of CN119563023A publication Critical patent/CN119563023A/zh
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance

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  • Crystallography & Structural Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN202380043499.1A 2022-04-29 2023-04-28 用于crispr指导rna的保护性寡核苷酸 Pending CN119563023A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202263336340P 2022-04-29 2022-04-29
US63/336,340 2022-04-29
US202363438842P 2023-01-13 2023-01-13
US63/438,842 2023-01-13
PCT/US2023/020418 WO2023212327A1 (en) 2022-04-29 2023-04-28 Protecting oligonucleotides for crispr guide rna

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CN119563023A true CN119563023A (zh) 2025-03-04

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CN202380043499.1A Pending CN119563023A (zh) 2022-04-29 2023-04-28 用于crispr指导rna的保护性寡核苷酸

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US (1) US20230407295A1 (enExample)
EP (1) EP4514972A1 (enExample)
JP (1) JP2025514327A (enExample)
CN (1) CN119563023A (enExample)
AU (1) AU2023262033A1 (enExample)
CA (1) CA3250967A1 (enExample)
WO (1) WO2023212327A1 (enExample)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6312894B1 (en) * 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US9340799B2 (en) * 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
EP3835419A1 (en) * 2013-12-12 2021-06-16 The Regents of The University of California Methods and compositions for modifying a single stranded target nucleic acid
EP3985115A1 (en) * 2014-12-12 2022-04-20 The Broad Institute, Inc. Protected guide rnas (pgrnas)
WO2016130943A1 (en) * 2015-02-13 2016-08-18 Rana Therapeutics, Inc. Hybrid oligonucleotides and uses thereof
US11845929B2 (en) * 2016-07-08 2023-12-19 Ohio State Innovation Foundation Modified nucleic acids, hybrid guide RNAs, and uses thereof
EP3781677A4 (en) * 2018-04-16 2022-01-19 University of Massachusetts COMPOSITIONS AND METHODS FOR IMPROVED GENE EDITTING

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CA3250967A1 (en) 2023-11-02
JP2025514327A (ja) 2025-05-02
WO2023212327A1 (en) 2023-11-02
AU2023262033A1 (en) 2024-12-12
EP4514972A1 (en) 2025-03-05
US20230407295A1 (en) 2023-12-21

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