CN119432744A - 使神经细胞分化的方法及相关组合物和使用方法 - Google Patents

使神经细胞分化的方法及相关组合物和使用方法 Download PDF

Info

Publication number
CN119432744A
CN119432744A CN202411614157.3A CN202411614157A CN119432744A CN 119432744 A CN119432744 A CN 119432744A CN 202411614157 A CN202411614157 A CN 202411614157A CN 119432744 A CN119432744 A CN 119432744A
Authority
CN
China
Prior art keywords
cells
day
cpm
pluripotent stem
therapeutic composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202411614157.3A
Other languages
English (en)
Chinese (zh)
Inventor
A·布拉特-莱尔
J·洛林
H·特伦
R·威廉姆斯
J·莫斯曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aspen Neuroscience
Original Assignee
Aspen Neuroscience
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aspen Neuroscience filed Critical Aspen Neuroscience
Publication of CN119432744A publication Critical patent/CN119432744A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Neurosurgery (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Psychology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
CN202411614157.3A 2020-01-13 2021-01-13 使神经细胞分化的方法及相关组合物和使用方法 Pending CN119432744A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202062960669P 2020-01-13 2020-01-13
US62/960,669 2020-01-13
CN202180020647.9A CN115335511A (zh) 2020-01-13 2021-01-13 使神经细胞分化的方法及相关组合物和使用方法
PCT/US2021/013324 WO2021146349A1 (en) 2020-01-13 2021-01-13 Method of differentiating neural cells and related compositions and methods of use

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN202180020647.9A Division CN115335511A (zh) 2020-01-13 2021-01-13 使神经细胞分化的方法及相关组合物和使用方法

Publications (1)

Publication Number Publication Date
CN119432744A true CN119432744A (zh) 2025-02-14

Family

ID=74557262

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202411614157.3A Pending CN119432744A (zh) 2020-01-13 2021-01-13 使神经细胞分化的方法及相关组合物和使用方法
CN202180020647.9A Pending CN115335511A (zh) 2020-01-13 2021-01-13 使神经细胞分化的方法及相关组合物和使用方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202180020647.9A Pending CN115335511A (zh) 2020-01-13 2021-01-13 使神经细胞分化的方法及相关组合物和使用方法

Country Status (12)

Country Link
US (1) US20230059010A1 (https=)
EP (1) EP4090734B1 (https=)
JP (2) JP7669048B2 (https=)
CN (2) CN119432744A (https=)
AU (2) AU2021209068B2 (https=)
BR (1) BR112022013843A2 (https=)
CA (1) CA3167713A1 (https=)
ES (1) ES3008210T3 (https=)
IL (1) IL294565B2 (https=)
MX (1) MX2022008625A (https=)
SA (1) SA522433282B1 (https=)
WO (1) WO2021146349A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023004366A1 (en) * 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Transposon-based modulation of gba1 and related compositions and uses thereof
WO2023004370A1 (en) * 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Aav-based modulation of gba1 and related compositions and uses thereof
WO2023137200A2 (en) * 2022-01-13 2023-07-20 The Board Of Trustees Of The Leland Stanford Junior University Generation of neural organizer organoids and midline assembloids from human pluripotent stem cells
JP2025512359A (ja) * 2022-04-11 2025-04-17 リサーチ ディベロップメント ファウンデーション 分化した神経細胞を作出および使用するための方法
WO2023201361A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
US12565639B2 (en) 2023-06-13 2026-03-03 Aspen Neuroscience, Inc. Methods for differentiating dopaminergic neurons from stem cells
US20250157580A1 (en) 2023-11-13 2025-05-15 Aspen Neuroscience, Inc. Methods of predicting dopamine production capability of differentiated neuronal cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104379732A (zh) * 2012-04-24 2015-02-25 国际干细胞公司 由人多能干细胞衍生神经干细胞和多巴胺能神经元
CN105849255A (zh) * 2013-09-05 2016-08-10 国立大学法人京都大学 新的产多巴胺神经前体细胞诱导方法
US20190112575A1 (en) * 2016-04-22 2019-04-18 Kyoto University Method for producing dopaminergic neuron progenitor cell

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5033252A (en) 1987-12-23 1991-07-23 Entravision, Inc. Method of packaging and sterilizing a pharmaceutical product
US5052558A (en) 1987-12-23 1991-10-01 Entravision, Inc. Packaged pharmaceutical product
US5323907A (en) 1992-06-23 1994-06-28 Multi-Comp, Inc. Child resistant package assembly for dispensing pharmaceutical medications
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
WO2002014555A2 (en) 2000-08-11 2002-02-21 University Of Utah Research Foundation Single-labeled oligonucleotide probes
ITCZ20020002A1 (it) 2002-04-11 2003-10-13 Parco Scient E Tecnologico Del Dispositivo e metodo per il rilevamento simultaneo di differenti anticorpi e antigeni in campioni clinici, alimentari ed ambientali
WO2013187416A1 (ja) * 2012-06-12 2013-12-19 学校法人 慶應義塾 神経系分化に適したiPS細胞の増幅方法、及び神経幹細胞の誘導方法
AU2014352773B2 (en) * 2013-11-21 2021-04-29 Memorial Sloan-Kettering Cancer Center Specification of functional cranial placode derivatives from human pluripotent stem cells
ES2939807T3 (es) * 2014-03-21 2023-04-27 Fujifilm Cellular Dynamics Inc Producción de neuronas dopaminérgicas del mesencéfalo y métodos para su utilización
WO2017160234A1 (en) * 2016-03-14 2017-09-21 Agency For Science, Technology And Research Generation of midbrain-specific organoids from human pluripotent stem cells
US20210123017A1 (en) * 2017-04-19 2021-04-29 National University Corporation Nagoya University Method for producing dopaminergic neurons
EP3715450A4 (en) * 2017-11-24 2021-08-25 Sumitomo Chemical Company, Limited METHOD OF PREPARING CELL MASS, INCLUDING HYPOPHYSICAL TISSUE, AND CELL MASS THEREOF
ES2968737T3 (es) * 2018-11-06 2024-05-13 Juno Therapeutics Inc Proceso para producir células T manipuladas genéticamente

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104379732A (zh) * 2012-04-24 2015-02-25 国际干细胞公司 由人多能干细胞衍生神经干细胞和多巴胺能神经元
CN105849255A (zh) * 2013-09-05 2016-08-10 国立大学法人京都大学 新的产多巴胺神经前体细胞诱导方法
US20190112575A1 (en) * 2016-04-22 2019-04-18 Kyoto University Method for producing dopaminergic neuron progenitor cell

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
徐如祥: "神经干细胞", 31 December 2006, 军事医学科学出版社, pages: 52 *

Also Published As

Publication number Publication date
MX2022008625A (es) 2022-09-09
EP4090734B1 (en) 2024-11-06
AU2021209068A1 (en) 2022-07-28
CA3167713A1 (en) 2021-07-22
JP2025118628A (ja) 2025-08-13
IL294565A (en) 2022-09-01
EP4090734C0 (en) 2024-11-06
CN115335511A (zh) 2022-11-11
EP4090734A1 (en) 2022-11-23
JP2023512453A (ja) 2023-03-27
IL294565B2 (en) 2025-04-01
BR112022013843A2 (pt) 2022-09-13
AU2024278587A1 (en) 2025-01-16
IL294565B1 (en) 2024-12-01
ES3008210T3 (en) 2025-03-21
SA522433282B1 (ar) 2025-04-21
AU2021209068B2 (en) 2024-09-19
WO2021146349A1 (en) 2021-07-22
JP7669048B2 (ja) 2025-04-28
US20230059010A1 (en) 2023-02-23

Similar Documents

Publication Publication Date Title
AU2021209068B2 (en) Method of differentiating neural cells and related compositions and methods of use
US20240263134A1 (en) Midbrain dopamine (da) neurons for engraftment
WO2006091766A2 (en) Human trophoblast stem cells and use thereof
AU2017214749B2 (en) Methods of differentiating stem cell-derived ectodermal lineage precursors
CN112601814A (zh) 包含嗅神经细胞或其前体细胞的细胞团块及其制备方法
JP2023075336A (ja) 幹細胞由来外胚葉系統前駆体を分化する方法
US20250283034A1 (en) Methods for differentiating dopaminergic neurons from stem cells
US20240329032A1 (en) Methods of differentiating neural cells and predicting engraftment thereof and related compositions
US20230165909A1 (en) Gene editing of lrrk2 in stem cells and method of use of cells differentiated therefrom
CN119384700A (zh) 细胞分化状态的分类方法和分化细胞的相关组合物
WO2021016496A2 (en) Methods to reprogram somatic cells to alternative cell fates or primitive cell states
US20230081881A1 (en) Transposon-based modulation of gba1 and related compositions and uses thereof
HK40082138B (en) Method of differentiating neural cells and related compositions and methods of use
HK40082138A (en) Method of differentiating neural cells and related compositions and methods of use
US20250382572A1 (en) Automated methods for differentiating dopaminergic neurons from stem cells
CN117769591A (zh) 源自人多能干细胞的大脑皮质细胞制剂的制造方法
US20250340837A1 (en) Cells

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination