CN1189189A - Method for liquefying starch - Google Patents
Method for liquefying starch Download PDFInfo
- Publication number
- CN1189189A CN1189189A CN96195006A CN96195006A CN1189189A CN 1189189 A CN1189189 A CN 1189189A CN 96195006 A CN96195006 A CN 96195006A CN 96195006 A CN96195006 A CN 96195006A CN 1189189 A CN1189189 A CN 1189189A
- Authority
- CN
- China
- Prior art keywords
- sodium
- starch
- starch solution
- αdian fenmei
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
According to the invention a method is provided for liquefying starch comprising the steps of adding a sodium composition to the starch prior to or simultaneously with liquefying the starch; adding alpha -amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Preferred sodium compositions comprise sodium chloride, sodium bicarbonate, sodium benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, sodium nitrate, sodium tartrate, sodium tetraborate, sodium propionate, sodium citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or a mixture thereof.
Description
The present invention relates to improve αDian Fenmei in the application that cereal starch is converted in derived product such as glucose, fructose and the alcohol.Specifically, the present invention relates to before the liquefaction or during the liquefaction sodium ion is being added starch mixture to improve at low pH, i.e. the efficient that when≤about pH5.9, liquefies.
Cereal such as corn are used as a kind of starch source for a long time.Known separation of people and purifying starch are wet milling processs with one of method of being used for the industrial processes process, this method has developed into highly narrow spectrum and comprehensive system, be designed to as far as possible fully separate main component in the grain (referring to Stanley A.Watson, Starch:Chemistry ﹠amp; Technology, Vol.II, IndustrialAspects, Academic Press, New York, 1967, pp.30-51).
In wet milling process commonly used, be used to produce the first immersion treatment process that is called as dipping that experiences of dried grains of starchbased product.During dipping, cereal is subjected to the adverse current flow action to separate multiple solute, comprises phytate (phytate) and phytic acid, carbohydrate, salt and the protein of cereal-granules.From soak water (steep water), separate the grain that soaked then, carry out Mechanical Crushing and grind operation.Use the plumule in flotation and the centrifuging separating starch then, fiber and protein; Again gained endosperm (starch), fiber and proteinic slurry are further ground and screen to isolate fiber.At last, pass through back flushing and isolated protein and endosperm related component, by centrifugation separating starch from protein/gluten stream based on density.Fully the isolating starch flow of flushing comprises solute such as inorganic salt and compound such as phytate and phytate to remove the relevant solute of any on-granulated starch again.Products therefrom is the insoluble granular starch slurry of highly purified mistake, and it is used as the starting product of saccharification to fructose.
Usually, the course of processing from starch to fructose was made of four steps: the liquefaction granular starch, the starch that saccharification has been liquefied forms glucose, purifying and be isomerizated into fructose.The purpose of starch liquefacation processing treatment is that spissated starch polymer particle suspension liquid is changed into low viscosity, the shorter soluble dextrins solution of chain length.This step becomes glucose or other sugar quite important for processing easily with standard equipment with saccharification effectively.For the granular starch that liquefies, the temperature that require to improve granular starch to be higher than about 72 ℃ with the gelatinization particle, destroy the insoluble starch particle immediately during heat treated and produce water soluble starch solution.Use αDian Fenmei (E3.2.1.1.) the dissolved starch solution that liquefies again.
Common enzymatic liquefaction processing process comprises: pH to 6.0～6.5 of regulating the granular starch slurry, this pH is suitable for deriving from the αDian Fenmei of Bacillus licheniformis (Bacillus licheniformis), regulates pH by adding calcium hydroxide, sodium hydroxide or yellow soda ash.The advantage that adds calcium hydroxide is also to provide calcium ion, and known calcium ion energy stable alpha amylase is with anti-inactivation.When adding αDian Fenmei, with the suspension pumping by vapor-nozzle so that be warming up to 80 °～115 ℃ immediately.Starch is by rapidly gelatinization, and owing to there is αDian Fenmei, makes the starch depolymerization and must be easy to the flowing material of pumping by the random hydrolyzing alpha of αDian Fenmei (1-4) glycosidic link.
In second kind of version of liquefaction processing, αDian Fenmei is added starch suspension, this suspension is maintained under 80-100 ℃ the temperature with hydrolyzed starch particle partly, again the starch suspension pumping after the partial hydrolysis is higher than about 105 ℃ nozzle with any residual granular texture of complete gelatinization by temperature.After treating the pasted starch cooling, can add αDian Fenmei for the second time with further hydrolyzed starch.
The third version of this treating processes is called the processing of dry method abrasive dust.In the dry method abrasive dust, will put in order that grain grinds and mix with water.Can remove degerming by flotation separation or suitable technology arbitrarily; With αDian Fenmei liquefaction gained mixture, other composition of wherein starch-containing, fiber, protein and cereal.When adopting the dry method milling process, common actually operating is to carry out enzymatic liquefaction at a lower temperature in this area.It is generally acknowledged that when Dian Fentang was changed into soluble dextrins, low-temperature liquefaction was effective not as high temperature liquefaction.
Normally, in the presence of αDian Fenmei, the starch solution after the gelatinization is remained on higher temperature until the DE that reaches 10-20, generally need 1～3 hour.Dextrose equivalent value (DE) is an industrial standards of weighing total reducing sugars concentration, is based on that dry weight calculates with D-glucose.The DE of unhydrolysed granular starch is actually zero, and the DE of definition D-glucose is 100.
The retainable top temperature of starch solution that contains αDian Fenmei is decided by the microbial source that obtains enzyme and the molecular structure of αDian Fenmei molecule.Usually be not higher than about 90 ℃ from the αDian Fenmei use temperature of wild-type subtilis (B.Subtilis) bacterial strain or the production of bacillus amyloliquefaciens (B.amyloliquefaciens) bacterial strain, because can cause too fast heat inactivation when being higher than this temperature; And can under up to about 110 ℃ temperature, use from the αDian Fenmei of wild-type lichem bacillus strain production.
But the existence stable alpha amylase of known starch and calcium ion is with anti-inactivation.Yet αDian Fenmei uses in case too fast inactivation under greater than 6 pH.At low temperatures, the known αDian Fenmei that derives from Bacillus licheniformis demonstrates good hydrolyzed starch substrate activity low under 5 pH.But, when this enzyme was used in the i.e. hydrolysis of 102 ℃～109 ℃ of following starch of common nozzle temperature, pH must maintain at least greater than pH5.7 to avoid too fast inactivation.Regrettably this pH requires to provide the processing machine meeting of close limit, because the pH value can produce unwanted by product such as Fructus Hordei Germinatus oligose class greater than 6.0.Therefore, the pH that in fact liquefies must remain on 5.9～6.0 to obtain the hydrolyzed starches of desirable yield.
In relevant when liquefaction,, another problem of pH was requirement with the pH of starch suspension from about 4, and it is the pH that derives from the W-Gum suspension in wet-milling stage, brings up to 5.9～6.0.Need in this pH regulator to add expensive sour neutralizing chemicals, but also require extra ion-exchange to make with extra care last mashing product to remove pharmaceutical chemicals.In addition, next step processing after the liquefaction generally is that liquefying starch saccharification is become glucose, and requiring pH is 4～4.5; Therefore, pH must be lowered to 4～4.5 from 5.9～6.0; Need to add in addition pharmaceutical chemicals and extra purification step.
In U.S. Patent No. 5,322, in 778, time finish liquefaction in pH4.0～6.0 by in liquefaction slurry, adding following substances: antioxidant such as hydrosulphite, xitix or its salt, saccharosonic acid, or phenol antioxidant such as butylated hydroxyanisol, Yoshinox BHT or alpha-tocopherol.According to the claim of this invention, must add the antioxidant of concentration greater than 5mM.
In U.S. Patent No. 5,180, in 669, be to realize the liquefaction of between pH5.0～6.0 by in the ground starch slurry, adding the carbanion that is in excess in this solution aequum of buffering.Cause that pH increases owing to add carbanion, can generate hydrionic material for example mineral acid example hydrochloric acid or sulfuric acid neutralize so will add usually in the slurry.
In PCT Publication No.WO94/02597, the improved mutant ' alpha '-amylase of oxidative stability has been described, wherein one or more methionine(Met) are replaced by the arbitrary amino acid except that halfcystine or methionine(Met).
In PCT Publication No.94/18314, the improved mutant ' alpha '-amylase of oxidative stability has been described, wherein one or more methionine(Met), tryptophane, halfcystine, Histidine or tyrosine residues are by non-oxidizable aminoacid replacement.
In PCT Publication No.WO91/00353, studied the related problems that solve when liquefaction by genetically engineered processing αDian Fenmei, comprise that characteristic is as higher thermostability, acid acceptance and alkaline stability.
In U.S. Patent No. 4,914, in 029, in the concentrated solution of corn soaking, add phytase with the phytic acid amount in the concentrated solution that reduces corn soaking, so more effectively the corn steeping mother liquor is used for animal-feed.
Although obtained progress in the prior art, but still need under low pH value, use the effective ways that commercially available αDian Fenmei carries out starch liquefacation.
An object of the present invention is, use facile mutant or wild-type αDian Fenmei and provide better efficient for low pH liquefying starch.
Further purpose of the present invention is to provide and need not add expensive antioxidant and the method for liquefying starch.
According to the present invention, the method for liquefied starch soln comprises following a few step: (a) in the liquid of starch shallow lake, add composition of sodium, wherein in the starch solution final na concn greater than 20ppm; (b) in starch solution, add αDian Fenmei; (c) will comprise the starch solution insulation certain hour of composition of sodium and αDian Fenmei, and be under the condition of the starch solution that is suitable for starch solution is liquefied as liquefaction, to be incubated; The condition of carrying out step (c) makes and is present in the ultimate density of antioxidant in the starch solution less than 2.5mM.According to the preferred embodiment of the invention, the ultimate density of composition of sodium is about 20ppm or bigger; Preferred about 20ppm～about 10,000ppm; 50ppm～about 1000ppm more preferably from about; And 100～500ppm most preferably from about.Step (c) is also preferably carried out being lower than under about 5.9 the pH; More preferably less than about 5.7; And most preferably be lower than about 5.5.
As more detailed pointing out hereinafter, implement the present invention and can give commercialization starch liquefacation method with important advantage.Although do not think bound by theory, the applicant thinks that still adding composition of sodium can eliminate the related problems that hang down the pH liquefying starch with αDian Fenmei, and this is owing to make the effect of unsettled one or more compositions of starch in the raising of enzyme stability itself or sodium and the starch solution.The applicant is surprised to find, and the sodium of adding gives αDian Fenmei bigger low pH activity, and this just can be lower than the granular starch that liquefies effectively under about 5.9 the pH value with commercially available αDian Fenmei.
With reference to following detailed description also in conjunction with the accompanying drawings, just can understand the present invention and further purpose and attendant advantages better.
" liquification " or " liquefaction " expression starch is converted to the course of processing of the dextrin that chain length is shorter, viscosity is littler.When this process generally comprises starch pasting or add αDian Fenmei subsequently.In industrial processes, granular starch preferably derives from the source that comprises the broken wheat of corn, wheat, milo, jowar, rye or parch (bulgher).But, the present invention can be applicable to be suitable for any cereal starch source of liquification, for example known other any cereal or plant origin that can produce the starch that is suitable for liquification.
The liquid that therefrom extracts during " immersion concentrated solution " expression dipping paddy particle.Soak the cereal soluble components that concentrated solution contains significant quantity.
The water-msoluble ingredients of " granular starch " or " starch granules " expression edible cereal, be remove by cereal wet-millings such as dipping, Mechanical Crushing, separation, screening, back flushing and centrifugation processing exemplary process steps shell, fiber, protein, fat, plumule and solute resistates afterwards.Granular starch comprises complete starch granules, and it almost only contains the starch molecule (being amylopectin and amylose starch) of filling.For corn, the granular starch component comprises about 99% starch, remaining 1% contain protein, fat, ash content, fiber and with the closely-related trace ingredients of particle.The packing structure of granular starch seriously blocks the amylatic ability of αDian Fenmei.The gelatinization of starch can destroy particle and form Zulkovsky starch solution and help enzymically hydrolyse.
" starch solution " expression heating granular starch and the water-soluble pasted starch that produces.When heated particle after be higher than about 70 ℃, granular starch decomposes and forms the water soluble mixt of loose starch molecule.For example, for yellow dent corn, comprise that this mixture of about 75% amylopectin and 25% amylose starch forms viscous solution in water.In the commercialization course of processing that generates glucose or fructose, be that starch solution liquefaction is formed soluble dextrins solution.
" αDian Fenmei " expression is decomposed or the hydrolysis enzymic activity of α (1-4) glycosidic link in starch, amylopectin or the amylose starch polymkeric substance for example.Suitable αDian Fenmei have natural αDian Fenmei and be applicable to starch liquefacation reorganization or mutant amylase.Preferred amylase has and derives from bacillus and specifically be the αDian Fenmei that derives from Bacillus licheniformis, bacillus amyloliquefaciens or bacstearothermophilus among the present invention.
" composition of sodium " or " sodium " expression contains the composition or the mixture of sodium, when its water-soluble solution, causes existing free sodium ion.Composition of sodium can be free sodium ion, sodium metal, contain the salt of sodium or can introduce other art-recognized any means of free sodium ion in the aqueous solution.Suitable sodium salt comprises sodium-chlor, sodium bicarbonate, Sodium Benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, SODIUMNITRATE, sodium tartrate, sodium tetraborate, Sodium Propionate, Trisodium Citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or its mixture.
The ultimate density of composition of sodium in starch solution should be the concentration that is enough to efficient liquefaction starch according to the present invention usually.The ultimate density that contains composition of sodium in the adding starch is 20ppm or bigger normally; Preferably about 20ppm～about 10,000ppm; 50ppm～about 1000ppm more preferably from about; And 100～500ppm most preferably from about.
Starch liquefacation of the present invention makes liquefaction reaction, and promptly the enzymically hydrolyse of starch, amylopectin or amylose starch is compared with the prior art liquifying method, less than 5.9 or even less than 5.5 pH under carry out effectively.Liquefaction reaction preferably carries out under less than 5.9 pH; More preferably between about 4.5～about 5.7; And most preferably between about 4.5～about 5.5.
The temperature range of liquification generally is known to the effectively any liquefaction temperature of starch liquefacation.The temperature of starch preferably between about 80 ℃～about 115 ℃, more preferably from about 100 ℃～about 110 ℃, and most preferably from about 105 ℃～about 108 ℃.
In embodiment preferred of the present invention, before adding αDian Fenmei and liquefying starch or in granular starch or starch solution, add compounds containing sodium simultaneously.Press known technology then, under suitable pH and suitable temperature, slurry is incubated reasonable time to carry out starch liquefacation.According to the present invention, by promptly adding composition of sodium, the ability of the efficient liquefaction starch that has been greatly improved prior to adding αDian Fenmei prior to liquefaction.
Following embodiment is illustrative rather than restrictive, and application of the present invention has some advantages.Yet those of ordinary skills should be able to replace condition in the above-mentioned open explanation, cereal, temperature, enzyme or the like.
Embodiment
Embodiment 1
The assay determination alpha-amylase activity
By the activity of assay determination αDian Fenmei, this analysis forms the ability of blue complex based on starch and iodine and the disappearance of this color after starch is hydrolyzed into shorter dextrin molecule.To produce the required digestion time definition alpha-amylase activity of colour-change, certain state that colour-change indication starch paste is wherein refined.
Agents useful for same is as follows: be diluted to about 2 liters after phosphate buffered saline buffer-with potassium primary phosphate (340g) and sodium hydroxide (25.3g) is water-soluble.This damping fluid is cooled to room temperature, regulates pH to 6.2 ± 0.1.Dilution buffer liquid to 2 liter in volumetric flask.Starch substrates-10 gram (dry-matter) solubility Lin Tena starch suspensions are washed about 300ml boiling water behind 50ml water.Again this suspension is boiled and under ceaselessly stirring, boiled 5 minutes.This starch solution of cooling adds the 125ml phosphate buffered saline buffer to room temperature under constantly stirring.This solution of dilute with water is to 500ml.Stock iodine solution-with iodine crystal (5.5g) and potassiumiodide (11.0g) water-soluble after, in volumetric flask, be diluted to 250ml.This solution is kept in Dark Place.Liquor iodi mitis-with potassiumiodide (20g) and 2ml stock iodine solution water-soluble after, in volumetric flask, be diluted to 500ml.Enzyme diluting soln-calcium chloride (11.1g) is dissolved in 4 premium on currency.Water of using in all reagent or distilled water or deionized water.
With the enzyme diluting soln active αDian Fenmei sample to be measured is diluted to 10-15LU/ml (as following definition).To many commercialization αDian Fenmei preparations, suitable extent of dilution is 2000 times.5 ml aliquots liquor iodi mitises are dispensed into 13 * 100mm test tube, and get the 10ml starch substrates and place 23 * 200mm test tube.All test tubes all place 30 ℃ of water-baths.Carry out reading with the Hellige colorimetric card that special αDian Fenmei colour table (catalog number (Cat.No.) 620-s5) is housed.5 milliliters of enzymes that diluted (also under 30 ℃) are mixed with starch substrates and pick up counting.With reasonable time at interval, for example be spaced apart 1 minute in early days and the reaction later stage is spaced apart 15 seconds, change branch enzyme-substrate mixtures such as 1ml over to contain the liquor iodi mitis of regulating test tube in reaction.This starch iodine solution mixes the rectangular pipe (squaretube) that the back is changed over to the 13mm precision, and with the standard αDian Fenmei colour table colorimetric in the Hellige colorimetric card.When near terminal point, with sampling at interval in 0.25 minute.
Record sample of color and quite required time of colour table color, and be calculated as follows activity (the liquefon numerical table with every gram or every ml shows):
Wherein: the LU=liquefon
V=enzyme volume (5ml)
T=dextrinizing time (branch)
The D=dilution factor: the dilution volume is divided by the milliliter number or the gram number of oneself dilution enzyme.
Embodiment 2
Starch liquefacation condition-mensuration liquefying starch DE (dextrose equivalent value)
Utilize this reactor to carry out starch liquefacation: with 50 ft diams is that 0.24 inch (0.21 inch i.d.) tubular material of stainless steel curves diameter and is about 10 inches high about 5.5 inches coils.This coil is equipped with one 11.5 inches online static mixer (Cole-Parmer#G-04669-60), is fixed on from the about 4 feet places of front end.An online adjustable pressure-reducing valve of Swagelok (#SS-4CA-3) is housed in the coil rear end, sets opening pressure and be about 20psi.With piston type dosing pump with the speed of about 70ml/min to the coil starch size of packing into.The coil immersion is heated to glycerine-water-bath of 105.5 ℃ and heats usefulness recirculation heater/temperature regulator (Fisher Scientificmodel 7305) maintenance bath temperature.
Granular starch derives from wet milling of corn and used in two days.Another starch source LO-DEX
TX10 (by the water-soluble purifying dextrin of W-Gum limit hydrolysis generation) are available from AmericanMaize-Products Company, Hammond, Indiana.LO-DEX used herein
TM10 initial DE is about 9.5.
With deionized water thinned starch or Star Dri 5 required solid content to about 30-35% dry-matter, and with 2.5%NaOH or 6%HCl adjusting pH to desirable value.With CaCl
22H
2The form of O adds calcium.Typical liquefaction condition is:
Starch or LO-DEX
TM10 32%～35% solidss
Calcium 40～60ppm (30ppm is what add)
pH?????????????????????????5.0～6.0
αDian Fenmei 12～14LU/g sugar (butt)
Add in the reactor with about 70ml/min and to contain enzyme and CaCl
22H
2The starch of the calcium of O form or LO-DEX
TM10.Reactor is immersed the temperature of maintenance reactor in glycerine-water-bath at 105.5 ℃.Changing amyloid over to 95 ℃ the second step liquefaction from reactor bathes and kept 90 minutes.Measure the starch liquefacation degree immediately after the liquefaction of second step, this is by the Standard Analytical Methods of the Member Companies ofthe Corn Refiners Association, Inc., sixth ed., method described in the AnalyticalProcedure Committee (1980) is measured by the dextrose equivalent value (DE) of measuring sample.
The liquefaction of starch also can be used water heater M103-M perforated steam spray, and (Hydro-Thermal Corp., Milwaukee WI), are equipped with 2.5 liters of delay coils (delay coil), and terminal vacuum breaker are housed behind mixing section.By the Moyno pump starch is pumped into perforated steam spray with about 360ml/min, provide steam by the 150psi steam-pipe, be decompressed to 90～100psi and the controlled slurry temperature that makes to about 105.5 ℃, this can be by the temperature sensor measurement at rear portion.Temperature sensor is installed in the about 5cm of about 10cm in water heater perforated steam spray back and vacuum breaker front place.
Embodiment 3
Positively charged ion under the low pH by the influence of αDian Fenmei liquefaction W-Gum
(Clinton lowa) starches with deionized water (about 23kg is in about 50 liters) furnishing dried corn starch for Clinton Brand 106-B Pearl corn starch, ADM CornProcessing, allows its aquation 16 hours.Filtration, resuspending wash starch 2 times in deionized water, to remove dissolved matter, be diluted to 35% solid substance with deionized water then.In starch, add calcium (with CaCl
22H
2O adds, and 50ppm), regulates pH to about 5.5 with 2.5%NaOH.Add about 5mM positively charged ion to be evaluated to starch size, and add αDian Fenmei (SPEZYME AA20 with the ratio of 12LU/g sugar, produce by lichem bacillus strain, can be from GenencorInternational, Inc. be purchased), add 2.5%NaOH or 6%HCl regulator solution pH to pH5.5 more as required.With the reactor assembly described in the embodiment 2 and this solution of method hydrolysis.The insulation back is measured the degree of hydrolysis of starch size immediately by measuring dextrose equivalent value (DE) for the second time.
Following table shows, during the W-Gum liquefaction, is stoped the situation of αDian Fenmei inactivation by the sodium salt that adds rather than other positively charged ion when pH5.5.
Table 1
Positively charged ion is to the influence of W-Gum liquefaction under the pH5.5
| Compound to be evaluated | ???????DE |
| Do not have | ??????4.9 |
| ?????????NaCl | ??????9.7 |
| ?????????LiCl | ??????3.0 |
| ?????????KCl | ??????6.5 |
| ????????NH 4Cl | ??????5.3 |
| ??????(NH 4) 2SO 4 | ??????5.4 |
| ????????CaCl 2 | ??????5.1 |
The applicant confirms that some positively charged ion (is K
+And NH
4 +) less effect with to add the less change amount of NaOH be used for pH regulator consistent.Yet the applicant thinks that less effect also can be provided by potassium ion, for example is approximately the 10-25% that composition of sodium causes effect.
Embodiment 4
The change of balance anion is to the influence of stable sodium αDian Fenmei
(Clinton lowa) starches with deionized water (about 23kg is in about 50 liters) furnishing dried corn starch for Clinton Brand 106-B Pearl corn starch, ADM CornProcessing, allows its aquation 16 hours.Filtration, resuspending wash starch 2 times in deionized water, to remove dissolved matter, be diluted to 35% solid substance with deionized water then.In starch, add calcium (with CaCl
22H
2O adds, and 50ppm), regulates pH to about 5.5 with 5%KOH.Adding various sodium salts extremely final na concn in starch size is 5mM.Add αDian Fenmei (SPEZYME AA20 is produced by lichem bacillus strain, can be, Inc. is purchased) with the ratio of 12LU/g sugar, add 5%KOH or 6%HCl regulator solution pH to pH5.5 more as required from Genencor International.With the reactor assembly described in the embodiment 2 and this solution of method hydrolysis.The insulation back is measured the degree of hydrolysis of starch size immediately by measuring dextrose equivalent value (DE) for the second time.
As shown in table 2, the inactivation of αDian Fenmei during the W-Gum liquefaction when sodium ion of adding different sources can stop pH5.5.But sodium bromate is obviously invalid, and this is because the intensive oxygenizement takes place between bromate and the protein.
Table 2
Sodium ion is the influence of αDian Fenmei stability during the time liquefaction to pH5.5
| Sodium salt to be evaluated | ?????DE |
| Do not have | ????2.3 |
| ?????NaH 2PO 4 | ????8.9 |
| Sodium Benzoate | ???11.7 |
| ?????Na 2SO 4 | ???10.7 |
| ?????NaNO 3 | ???10.8 |
| Seignette salt | ????9.8 |
| Sodium tetraborate | ???10.7 |
| Sodium Propionate | ???11.2 |
| Trisodium Citrate | ????6.6 |
| Sodium succinate | ???10.4 |
| Monosodium glutamate | ???10.6 |
| Sodium bromate | ????～0 |
Embodiment 5 sodium ions and αDian Fenmei concentration are to the influence of W-Gum liquefaction
To derive from the makers' corn starch liquid of wet method W-Gum with deionized water and be diluted to 35% solid substance, with Ca (OH)
2Form adds 30ppm calcium.In starch slurry, add various Na ion concentrations to required Na ion concentration.To each αDian Fenmei concentration, evaluate two Na ion concentrations at least, Na ion concentration is measured by sodium ion electrode (Corning#476138) in the starch slurry.With the starch slurry of the known solid substance of Whatman#3 filter paper filtering, press the na concn of the described mensuration of sodium electrode specification sheets filtrate.The Na ion concentration of report is represented with the umber in per 1,000,000 parts of whole starch slurries.
According to the required extremely about required pH (between 5.5 and 5.9) of pH that regulates starch slurry with 5%KOH or 6%HCl.With 12,15 or the ratio of the dried solid substance of 18LU/g sugar add αDian Fenmei (SPEZYME AA20, produce by lichem bacillus strain, can be from Genencor International, Inc. is purchased), the pH that looks required adding 5%KOH or 6%HCl regulator solution is to required pH.With the vapor-nozzle described in the embodiment 2 and this solution of method hydrolysis.After insulation for the second time, measure the degree of hydrolysis of starch slurry immediately by measuring dextrose equivalent value (DE).
For each αDian Fenmei consumption to be evaluated, regulate the Na ion concentration be used to liquefy, obtain greater than with less than 10 DE value.Measure then under each enzyme dosage and to produce the required Na ion concentration of DE 10 liquefying starchs, to the Na ion concentration described point, and between data point, use interpolation technique with the DE value.
As shown in table 3, pH reduces or along with the reduction of αDian Fenmei concentration, needs more sodium ions to produce the liquefying starch of DE10 along with when liquefaction.
Table 3
Produce the required na concn of DE10 liquification
| ????pH | Na concn (ppm) | ||
| ????12LU/g | ????15LU/g | ????18LU/g | |
| ????5.9 | ?????100 | ??????28 | ??????5 |
| ????5.7 | ?????155 | ??????95 | ?????22 |
| ????5.5 | ?????322 | ?????200 | ?????63 |
Certainly, should understand and to make large-scale changes and improvements to above-mentioned embodiment preferred.Therefore should treat above-mentioned detailed description like this, be intended to following claim, comprise that all equivalents limit scope of the present invention.
Claims (10)
1. the method for liquefied starch soln, it comprises the steps:
(a) add composition of sodium in described starch solution, the final na concn in the wherein said starch solution is greater than 20ppm;
(b) in described starch solution, add αDian Fenmei; With
(c) insulation contains this composition of sodium and this αDian Fenmei under the condition of described starch solution that is suitable for liquefying described starch solution reaches the starch solution that certain hour has liquefied with formation;
Carry out step (c) under certain condition, make that the ultimate density of the antioxidant that exists in the described starch solution is to contain to be less than the 2.5mM antioxidant.
2. the process of claim 1 wherein that described composition of sodium comprises: sodium-chlor, sodium bicarbonate, Sodium Benzoate, sodium sulfate, sodium bisulfite, sodium ascorbate, sodium acetate, SODIUMNITRATE, sodium tartrate, sodium tetraborate, Sodium Propionate, Trisodium Citrate, sodium succinate, monosodium glutamate, trisodium citrate, sodium phosphate or its mixture.
3. the process of claim 1 wherein that the described composition of sodium ultimate density that exists is about 20ppm～about 10,000ppm.
4. the process of claim 1 wherein that the described composition of sodium ultimate density that exists is about 50ppm～about 1000ppm.
5. the process of claim 1 wherein that the described composition of sodium ultimate density that exists is about 100ppm～about 500ppm.
6. the process of claim 1 wherein that described step (c) is to carry out under less than about 5.9 pH.
7. the process of claim 1 wherein that described step (c) is to carry out under about pH of 4.5～about 5.7.
8. the process of claim 1 wherein that described step (c) is to carry out under about pH of 4.5～about 5.5.
9. the process of claim 1 wherein that described step (a) is prior to described step (b).
10. the process of claim 1 wherein that described step (a) and described step (b) carry out simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96195006A CN1189189A (en) | 1995-06-02 | 1996-05-30 | Method for liquefying starch |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/459,984 | 1995-06-02 | ||
| CN96195006A CN1189189A (en) | 1995-06-02 | 1996-05-30 | Method for liquefying starch |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1189189A true CN1189189A (en) | 1998-07-29 |
Family
ID=5128947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96195006A Pending CN1189189A (en) | 1995-06-02 | 1996-05-30 | Method for liquefying starch |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1189189A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104520333A (en) * | 2012-08-09 | 2015-04-15 | 卡吉尔公司 | Process for starch liquefaction |
| CN113508142A (en) * | 2019-02-01 | 2021-10-15 | 瑞典淀粉生产者协会 | Process for preparing inhibited starch |
-
1996
- 1996-05-30 CN CN96195006A patent/CN1189189A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104520333A (en) * | 2012-08-09 | 2015-04-15 | 卡吉尔公司 | Process for starch liquefaction |
| CN113508142A (en) * | 2019-02-01 | 2021-10-15 | 瑞典淀粉生产者协会 | Process for preparing inhibited starch |
| CN113508142B (en) * | 2019-02-01 | 2023-03-17 | 瑞典淀粉生产者协会 | Process for preparing inhibited starch |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6184002B1 (en) | Method for liquefying starch | |
| EP0813607B2 (en) | Method for liquefying starch | |
| CN1111601C (en) | Mutant ' alpha '-amylase | |
| US20020022252A1 (en) | Use of enzymes to reduce steep time and SO2 requirements in a maize wet-milling process | |
| EP0579762A4 (en) | Liquefaction of granular-starch slurries using alpha-amylase in the presence of carbonate ion. | |
| US6586213B2 (en) | Process for clarification of xanthan solutions and xanthan gum produced thereby | |
| EP2152756B1 (en) | Method and composition for starch extraction and modification | |
| CN1189189A (en) | Method for liquefying starch | |
| Li et al. | Purification and characterisation of arabinoxylan arabinofuranohydrolase I responsible for the filterability of barley malt | |
| PL204435B1 (en) | ENZYMATIC TREATMENT OF CEREALS AND AN OAT PRODUCT WITH INCREASED GLUCOSE AND ß-GLUCAN CONTENT | |
| JP4964710B2 (en) | Process for producing β-glucan-containing cereal saccharified product | |
| Pérez‐Carrillo et al. | Cell wall degrading enzymes and proteases improve starch yields of sorghum and maize | |
| EP0610358A1 (en) | Liquefaction of dry milled starch grain slurries. | |
| CN1228444C (en) | Process for extraction of beta-amylase | |
| RU2443783C2 (en) | Method for production of feed hydrolysates of wheat grains | |
| Dicko et al. | Indigenous West African plants as novel sources of polysaccharide degrading enzymes: application in the reduction of the viscosity of cereal porridges | |
| Furegon et al. | Immobilization of rice limit dextrinase on γ-alumina beads and its possible use in starch processing | |
| Bertoft et al. | Characterisation of Dextrins Solubilised by α‐Amylase from Barley Starch Granules | |
| EP0129559A1 (en) | PREPARATION OF THE ENZYME $g(b)-GLUCANASE BY FERMENTATION OF FUNGI | |
| Brumm et al. | Isolation and Characterization of Starch‐Iodine Positive Material Formed During Saccharification of Corn Starch | |
| AU710097B2 (en) | Oat extract and process for producing it | |
| Tripathi et al. | Purification and partial characterization of α-amylase from soybean (Glycine max) | |
| MXPA97006706A (en) | Method for liquiding almi | |
| JP2012034582A (en) | Method for producing syrup | |
| AU2013202225A1 (en) | Method and composition for starch extraction and modification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| AD01 | Patent right deemed abandoned | ||
| C20 | Patent right or utility model deemed to be abandoned or is abandoned |