CN118591625A - 用于位点特异性基因编辑的丝氨酸重组酶系统 - Google Patents

用于位点特异性基因编辑的丝氨酸重组酶系统 Download PDF

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Publication number
CN118591625A
CN118591625A CN202380018612.0A CN202380018612A CN118591625A CN 118591625 A CN118591625 A CN 118591625A CN 202380018612 A CN202380018612 A CN 202380018612A CN 118591625 A CN118591625 A CN 118591625A
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CN
China
Prior art keywords
recombinase
variant
cell
bxb1
dna
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Pending
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CN202380018612.0A
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English (en)
Chinese (zh)
Inventor
S·阿朗根迪
F·A·福瑟
J·C·米勒
N·斯卡洛特
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Sangamo Therapeutics Inc
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Sangamo Therapeutics Inc
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Application filed by Sangamo Therapeutics Inc filed Critical Sangamo Therapeutics Inc
Publication of CN118591625A publication Critical patent/CN118591625A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/22—Endodeoxyribonucleases producing 3'-phosphomonoesters (3.1.22)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202380018612.0A 2022-01-28 2023-01-27 用于位点特异性基因编辑的丝氨酸重组酶系统 Pending CN118591625A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202263304565P 2022-01-28 2022-01-28
US63/304,565 2022-01-28
PCT/US2023/061500 WO2023147507A1 (en) 2022-01-28 2023-01-27 Serine recombinase systems for site-specific gene editing

Publications (1)

Publication Number Publication Date
CN118591625A true CN118591625A (zh) 2024-09-03

Family

ID=85328719

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380018612.0A Pending CN118591625A (zh) 2022-01-28 2023-01-27 用于位点特异性基因编辑的丝氨酸重组酶系统

Country Status (9)

Country Link
US (1) US20250243512A1 (https=)
EP (1) EP4469569A1 (https=)
JP (1) JP2025504019A (https=)
KR (1) KR20240135617A (https=)
CN (1) CN118591625A (https=)
AU (1) AU2023214005A1 (https=)
CA (1) CA3243345A1 (https=)
IL (1) IL314478A (https=)
WO (1) WO2023147507A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121693564A (zh) 2023-06-26 2026-03-17 夏威夷大学 进化的整合酶和其用于基因组编辑的方法
WO2025160202A1 (en) * 2024-01-22 2025-07-31 Arc Researrch Institute Engineered large serine recombinases
WO2025212976A2 (en) * 2024-04-03 2025-10-09 Sangamo Therapeutics, Inc. Engineered modular recombinase compositions, methods, and systems for site-specific dna recombination

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202006462D0 (en) * 2020-05-04 2020-06-17 Mote Res Limited Modifying genomes with integrase

Also Published As

Publication number Publication date
IL314478A (en) 2024-09-01
CA3243345A1 (en) 2023-08-03
JP2025504019A (ja) 2025-02-06
EP4469569A1 (en) 2024-12-04
WO2023147507A1 (en) 2023-08-03
AU2023214005A1 (en) 2024-08-15
KR20240135617A (ko) 2024-09-11
US20250243512A1 (en) 2025-07-31

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