CN118541493A - 在单一工作流程中检测遗传和表观遗传信息 - Google Patents
在单一工作流程中检测遗传和表观遗传信息 Download PDFInfo
- Publication number
- CN118541493A CN118541493A CN202280087109.6A CN202280087109A CN118541493A CN 118541493 A CN118541493 A CN 118541493A CN 202280087109 A CN202280087109 A CN 202280087109A CN 118541493 A CN118541493 A CN 118541493A
- Authority
- CN
- China
- Prior art keywords
- strands
- nucleic acid
- cytosine
- stranded dna
- cytosine conversion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163294640P | 2021-12-29 | 2021-12-29 | |
| US63/294,640 | 2021-12-29 | ||
| PCT/US2022/082476 WO2023129965A2 (en) | 2021-12-29 | 2022-12-28 | Detection of genetic and epigenetic information in a single workflow |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118541493A true CN118541493A (zh) | 2024-08-23 |
Family
ID=87000276
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202280087109.6A Pending CN118541493A (zh) | 2021-12-29 | 2022-12-28 | 在单一工作流程中检测遗传和表观遗传信息 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20250051840A1 (https=) |
| EP (1) | EP4457357A4 (https=) |
| JP (1) | JP2025502811A (https=) |
| CN (1) | CN118541493A (https=) |
| WO (1) | WO2023129965A2 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12509733B1 (en) | 2025-04-07 | 2025-12-30 | Inivata Ltd. | Methods and systems for detecting a primary disease |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025104430A1 (en) * | 2023-11-14 | 2025-05-22 | Tagomics Ltd | Profiling method for determining epigenetic modifications |
| WO2025128727A1 (en) * | 2023-12-12 | 2025-06-19 | Foundation Medicine, Inc. | Methods and systems for preparing a sequencing library |
| WO2025160495A1 (en) * | 2024-01-26 | 2025-07-31 | Cornell University | Hematopoietic stem cells and uses thereof in treating cancer |
| WO2025235365A1 (en) * | 2024-05-08 | 2025-11-13 | Freenome Holdings, Inc. | Methods and systems for improved methylation sequencing |
| WO2026022118A1 (en) | 2024-07-23 | 2026-01-29 | Qiagen Gmbh | Method of detecting methylated nucleic acids |
| WO2026043738A1 (en) * | 2024-08-20 | 2026-02-26 | Natera, Inc. | Improved method for methylation biomarker generation and analysis |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009132315A1 (en) * | 2008-04-24 | 2009-10-29 | Life Technologies Corporation | Method of sequencing and mapping target nucleic acids |
| WO2010048337A2 (en) * | 2008-10-22 | 2010-04-29 | Illumina, Inc. | Preservation of information related to genomic dna methylation |
| US20150011396A1 (en) * | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| NZ765943A (en) * | 2018-01-08 | 2022-10-28 | Ludwig Inst For Cancer Res Ltd | Bisulfite-free, base-resolution identification of cytosine modifications |
| CA3225385A1 (en) * | 2021-07-12 | 2023-01-19 | The Trustees Of The University Of Pennsylvania | Modified adapters for enzymatic dna deamination and methods of use thereof for epigenetic sequencing of free and immobilized dna |
-
2022
- 2022-12-28 EP EP22917534.4A patent/EP4457357A4/en active Pending
- 2022-12-28 JP JP2024539661A patent/JP2025502811A/ja active Pending
- 2022-12-28 CN CN202280087109.6A patent/CN118541493A/zh active Pending
- 2022-12-28 WO PCT/US2022/082476 patent/WO2023129965A2/en not_active Ceased
- 2022-12-28 US US18/722,508 patent/US20250051840A1/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12509733B1 (en) | 2025-04-07 | 2025-12-30 | Inivata Ltd. | Methods and systems for detecting a primary disease |
Also Published As
| Publication number | Publication date |
|---|---|
| US20250051840A1 (en) | 2025-02-13 |
| EP4457357A2 (en) | 2024-11-06 |
| EP4457357A4 (en) | 2026-01-07 |
| WO2023129965A3 (en) | 2023-10-05 |
| WO2023129965A2 (en) | 2023-07-06 |
| JP2025502811A (ja) | 2025-01-28 |
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