CN118339285A - 工程化Cas12b效应子蛋白及其使用方法 - Google Patents

工程化Cas12b效应子蛋白及其使用方法 Download PDF

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Publication number
CN118339285A
CN118339285A CN202280079229.1A CN202280079229A CN118339285A CN 118339285 A CN118339285 A CN 118339285A CN 202280079229 A CN202280079229 A CN 202280079229A CN 118339285 A CN118339285 A CN 118339285A
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China
Prior art keywords
cas12b
engineered
amino acid
nuclease
acid residues
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CN202280079229.1A
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Chinese (zh)
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李伟
周琪
陈阳灿
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Beijing Institute Of Stem Cell And Regenerative Medicine
Institute of Zoology of CAS
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Beijing Institute Of Stem Cell And Regenerative Medicine
Institute of Zoology of CAS
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    • C12N9/14—Hydrolases (3)
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    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
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CN202280079229.1A 2021-12-09 2022-12-09 工程化Cas12b效应子蛋白及其使用方法 Pending CN118339285A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2021136761 2021-12-09
CNPCT/CN2021/136761 2021-12-09
PCT/CN2022/137920 WO2023104185A1 (en) 2021-12-09 2022-12-09 Engineered cas12b effector proteins and methods of use thereof

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CN118339285A true CN118339285A (zh) 2024-07-12

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CN202211581644.5A Active CN116254246B (zh) 2021-12-09 2022-12-09 工程化cas12b效应蛋白及其使用方法

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US (1) US20250051742A1 (https=)
EP (1) EP4444875A4 (https=)
JP (1) JP2025501473A (https=)
CN (2) CN118339285A (https=)
WO (1) WO2023104185A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025067403A1 (zh) * 2023-09-28 2025-04-03 上海天泽云泰生物医药有限公司 用于治疗血红蛋白疾病的组合物和方法
TW202521691A (zh) * 2023-10-06 2025-06-01 美商藍岩醫療公司 經工程化之v型rna可程式核酸內切酶及其用途
WO2025096367A1 (en) * 2023-11-03 2025-05-08 University Of Florida Research Foundation, Inc. Thermostable and/or charge-engineered cas12a variants for nucleic acid detection
CN120330162A (zh) * 2023-12-06 2025-07-18 尧唐(上海)生物科技有限公司 一种Cas蛋白、包含其的CRISPR-Cas系统及其应用
WO2026060637A1 (en) * 2024-09-20 2026-03-26 Shanghai Vitalgen Biopharma Co., Ltd. Engineered mrna for gene editing using crispr/cas12b
CN120796267A (zh) * 2024-07-05 2025-10-17 上海天泽云泰生物医药有限公司 用于使用CRISPR/Cas系统进行基因编辑的经修饰的gRNA

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019127087A1 (zh) * 2017-12-27 2019-07-04 中国科学院动物研究所 基因组编辑系统和方法
PL3765615T3 (pl) * 2018-03-14 2023-11-13 Arbor Biotechnologies, Inc. Nowe enzymy i układy crispr ukierunkowane na dna
US20210292747A1 (en) * 2018-07-27 2021-09-23 The Regents Of The University Of Colorado, A Body Corporate Engineered chimeric nucleic acid guided nucleases compositions, methods for making, and systems for gene editing
CN113286884A (zh) * 2018-08-07 2021-08-20 博德研究所 新型cas12b酶和系统
EP3861112A4 (en) * 2018-10-04 2022-09-21 The Regents of the University of Colorado, A Body Corporate ENGINEERED CHIMERIC NUCLEIC ACID-GUIDED NUCLEAR CONSTRUCTS AND THEIR USES
BR112022007125A2 (pt) * 2019-10-17 2022-07-05 Pairwise Plants Services Inc Variantes de nucleases cas12a e métodos de produção e uso das mesmas
WO2021178934A1 (en) * 2020-03-06 2021-09-10 Metagenomi Ip Technologies, Llc Class ii, type v crispr systems
CN113308451B (zh) * 2020-12-07 2023-07-25 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
CN115851665B (zh) * 2021-05-27 2025-01-24 中国科学院动物研究所 工程化的Cas12i核酸酶及其效应蛋白以及用途
WO2022247873A1 (zh) * 2021-05-27 2022-12-01 中国科学院动物研究所 工程化的Cas12i核酸酶、效应蛋白及其用途

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Publication number Publication date
CN116254246A (zh) 2023-06-13
US20250051742A1 (en) 2025-02-13
EP4444875A4 (en) 2025-07-02
EP4444875A1 (en) 2024-10-16
WO2023104185A1 (en) 2023-06-15
CN116254246B (zh) 2025-09-23
JP2025501473A (ja) 2025-01-22

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