CN118330206A - Automatic operation instrument for poultry hemagglutination antibody experiment - Google Patents

Abstract

The invention relates to the technical field of detection experimental instruments, in particular to an automatic operation instrument for poultry hemagglutination antibody experiments, which solves the problem of low automation degree in the whole experimental process caused by the lack of automatic operation detection instruments for poultry hemagglutination antibody experiments in the existing market.

Description

Automatic operation instrument for poultry hemagglutination antibody experiment

Technical Field

The invention relates to the technical field of detection experimental instruments, in particular to an automatic operation instrument for an avian hemagglutination antibody experiment.

Background

Newcastle disease is a highly contagious disease caused by paramyxovirus, also called Asian fowl plague or pseudo fowl plague, and is often in acute septicemia, and is mainly characterized by dyspnea, loose stool, neurological disorder, mucous membrane and serosa bleeding, so that the newcastle disease is seriously harmful to chicken industry, and in order to detect pathogens of chickens, a hemagglutination antibody experiment is usually carried out on chickens, and the experimental steps comprise: sucking the Pbs buffer solution by a micropipette, sucking the newcastle disease standard antigen by the micropipette, sucking the unit virus by the micropipette, sucking the chicken erythrocyte suspension by the micropipette, dripping the solution into a miniature hemagglutination reaction plate according to the specified steps for reaction, beating the miniature hemagglutination reaction plate and a test tube to ensure that the solution is more uniformly mixed for reaction, and the like;

As can be seen from the above, a plurality of operation steps are required in the experiment of the poultry hemagglutination antibody, but the above steps are usually completed by manual operation, and the lack of automatic operation detecting instruments in the existing market results in low automation degree and high manual labor intensity in the whole experimental process.

Therefore, the invention provides an automatic operation instrument for poultry hemagglutination antibody experiments, so as to solve the problems.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides an automatic operation instrument for poultry hemagglutination antibody experiments, which solves the problems.

In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:

an automatic operation instrument for poultry hemagglutination antibody experiments comprises a base, an equipment box and a suspension arm;

And (2) base: the upper end of the base is sequentially provided with a liquid suction nozzle supply assembly, a reaction liquid storage assembly, a blood coagulation plate assembly and a residual liquid storage box along the length direction, a rectangular array in the liquid suction nozzle supply assembly is penetrated with disposable liquid suction nozzles, the liquid suction nozzle supply assembly, the reaction liquid storage assembly and the residual liquid storage box are all two and are arranged side by side from front to back, the blood coagulation plate assembly is divided into four and two rows, one end of the front and back two reaction liquid storage assemblies, which are close to each other, and one end of the front and back two blood coagulation plate assemblies, which are close to each other, are respectively fixed with a collision block, the collision blocks are staggered with each other, the base is internally provided with a first power component, the output end of the first power component is fixedly provided with a vibration arm component, the first power component drives the vibration arm component to move between the front liquid suction nozzle supply component and the rear liquid suction nozzle supply component, between the front reaction liquid storage component and the rear reaction liquid storage component and between the front blood coagulation plate component and the rear blood coagulation plate component, the vibration arm component knocks against the reaction liquid storage component and the front blood coagulation plate component and the rear blood coagulation plate component, the vibration arm component knocks against the liquid suction nozzle supply component, and a second sliding rail is fixed in the base along the length direction;

Equipment box: the equipment box moves on a second sliding rail, a second power assembly is arranged between the equipment box and the second sliding rail, the second power assembly drives the equipment box to move, a vacuum pump is fixed in the equipment box, and a lifting assembly is arranged in the equipment box;

Boom arm: the lifting arm is fixed at the output end of the lifting assembly, the lifting assembly drives the lifting arm to move up and down, the lifting arm moves above the liquid suction nozzle supply assembly, the reaction liquid storage assembly, the blood coagulation plate assembly and the residual liquid storage box, two groups of pipe penetrating holes are formed in the lower side of the lifting arm, each group of pipe penetrating holes respectively pass through the upper parts of the liquid suction nozzle supply assembly, the reaction liquid storage assembly, the blood coagulation plate assemblies and the residual liquid storage box, a clamping nozzle pipe vertically moves in each pipe penetrating hole, the upper end of the clamping nozzle pipe is communicated with the vacuum pump through a plastic pipe, a disposable liquid suction nozzle is movably sleeved at the lower end of the clamping nozzle pipe, the outer diameter of the upper end of the disposable liquid suction nozzle is larger than the diameter of each pipe penetrating hole, a nozzle pipe control assembly is arranged in the lifting arm, and the output end of the nozzle pipe control assembly is fixedly provided with the clamping nozzle pipe and drives the clamping nozzle pipe to move up and down and transversely.

Through the technical proposal, the mouth tube control component drives the mouth tube to move up and down, the mouth tube is inserted into the disposable liquid suction mouth in the liquid suction mouth supply component when moving downwards, the disposable liquid suction mouth is taken out when moving upwards, then the second power component drives the equipment box to move, the equipment box drives the suspension arm to move transversely, so that the disposable liquid suction mouth is respectively positioned above the reaction liquid storage component, the blood coagulation plate component and the residual liquid storage box, when the disposable liquid suction mouth is inserted downwards into the reaction liquid storage component, the vacuum pump works to form negative pressure in the mouth tube and the disposable liquid suction mouth, the pbs buffer solution, newcastle disease standard antigen, unit virus or chicken erythrocyte suspension in the reaction liquid storage component can be sucked, namely, the four steps of manually completing the suction of the pbs buffer solution by a micropipette, the suction of the newcastle disease standard antigen by the micropipette, the suction of the unit virus by the micropipette and the suction of the chicken erythrocyte suspension by the micropipette are replaced, when the disposable liquid suction nozzle is downwards inserted into the blood coagulation plate component, the vacuum pump is operated to form positive pressure in the mouth clamping pipe and the disposable liquid suction nozzle, so that the pbs buffer solution, newcastle disease standard antigen, unit virus or chicken erythrocyte suspension in the disposable liquid suction nozzle can be pushed out, namely, the step of dripping the solution into the blood coagulation plate is replaced by manual operation, when the disposable liquid suction nozzle is downwards inserted into or upwards pulled out of the blood coagulation plate component, the vacuum pump is operated to enable the mouth clamping pipe and the disposable liquid suction nozzle to circularly form positive pressure and negative pressure, the pbs buffer solution, newcastle disease standard antigen, unit virus or chicken erythrocyte suspension in the blood coagulation plate can be circularly sucked, the purpose of performing multiple dilution on the solution in the blood coagulation plate is realized, when the disposable liquid suction nozzle is positioned above the residual liquid storage box, the clamping nozzle pipe is completely retracted into the suspension arm to enable the disposable liquid suction nozzle to be forcedly separated from the clamping nozzle pipe, the disposable liquid suction nozzle after use falls into the residual liquid storage box to be temporarily stored, and the waste solution in the disposable liquid suction nozzle falls into the residual liquid storage box to be stored, so that the purpose of storing the waste disposable liquid suction nozzle and the residual liquid is achieved, the first power assembly drives the vibration arm assembly to move among the two liquid suction nozzle supply assemblies, the two reaction liquid storage assemblies and the two blood coagulation plate assemblies, and the vibration arm assembly can knock the reaction liquid storage assemblies and the blood coagulation plate assemblies, so that the solution mixing reaction in the reaction liquid storage assemblies and the blood coagulation plate assemblies is more uniform.

Preferably, the first power assembly comprises a fifth motor and a fifth screw, the fifth motor is fixed in the upper end of the base, the output end of the fifth motor is fixed with the fifth screw, the fifth screw rotates in the base, and the fifth screw is positioned between the front liquid suction nozzle supply assembly and the rear liquid suction nozzle supply assembly, between the front reaction liquid storage assembly and the rear reaction liquid storage assembly and between the front blood coagulation plate assembly and the rear blood coagulation plate assembly;

The vibration arm assembly comprises a toothed plate, a transverse moving seat, a gear and a seventh motor, wherein the fifth screw thread penetrates through the transverse moving seat, the transverse moving seat moves along the length direction of the base, the seventh motor is fixed on the side surface of the transverse moving seat, the gear is fixed after the output end of the seventh motor rotates to penetrate into the transverse moving seat, the gear is meshed with the middle end teeth of the toothed plate, and two ends of the toothed plate respectively penetrate out of two ends of the transverse moving seat in a movable mode;

through the technical scheme, the controller controls the fifth motor to work in an electrifying way, so that the fifth motor drives the fifth screw rod to rotate positively and negatively, the fifth screw rod drives the transverse moving seat to move left and right, the controller controls the seventh motor to work in an electrifying way, so that the seventh motor drives the gear to rotate positively and negatively, the gear drives the toothed plate to move back and forth through tooth meshing, the front liquid suction nozzle supply assembly, the front reaction liquid storage assembly and the front blood coagulation plate assembly are knocked when the toothed plate moves forward, the rear liquid suction nozzle supply assembly, the rear reaction liquid storage assembly and the rear blood coagulation plate assembly are knocked when the toothed plate moves backward, and therefore the purposes of knocking the liquid suction nozzle supply assembly, the reaction liquid storage assembly and the blood coagulation plate assembly are achieved.

Preferably, the blood coagulation plate assembly comprises blood coagulation plates, a limiting rod, a second spring, an inner supporting block, a third spring, a chassis and a mounting column, wherein long strip holes are formed in four corners of the chassis, the mounting column is transversely movably inserted in the long strip holes, the upper end of the mounting column supports the chassis, the third spring is fixed between the upper end of the mounting column and the side surface of the long strip holes, the lower end of the mounting column is fixed on the inner bottom side of the base, the limiting rod is transversely movably inserted in four corners of the upper end of the chassis, the moving directions of the limiting rod and the chassis are the same, the inner supporting block is fixed at the other end of the limiting rod, the second spring is fixed between the inner supporting block and the chassis, and the four inner supporting blocks are vertically movably clamped in the lower end of the blood coagulation plates;

an abutting block is fixed on the side surface of the chassis, and the toothed plate pushes the chassis to move transversely when pushing the abutting block on the chassis;

through above-mentioned technical scheme, with the downward cover of blood coagulation board outside four interior supporting shoe, the supporting shoe is in the second spring promotes for four interior supporting shoe block blood coagulation board, when the rack board in vibrations arm assembly strikes the conflict piece on the chassis, chassis lateral shifting, the chassis drives interior supporting shoe lateral shifting through the second spring, interior supporting shoe drives blood coagulation board lateral shifting, shakes the curdling to blood coagulation board promptly, makes the solution in the blood coagulation board carry out intensive mixing.

Preferably, the reaction liquid storage component comprises a first separation plate, a second separation plate, a reaction liquid storage box, a first supporting seat, a fourth spring, a residual liquid discharge pipe and a water pump, wherein two first supporting seats are arranged at the lower ends of the first supporting seats, the reaction liquid storage box is transversely movably inserted between the upper ends of the two first supporting seats, the fourth spring is fixed between the reaction liquid storage box and the first supporting seat, the lower side of the reaction liquid storage box passes through the residual liquid discharge pipe, the end part of the residual liquid discharge pipe is fixedly provided with the water pump, the second separation plate and the two first separation plates are vertically movably inserted in the reaction liquid storage box, and the second separation plate and the two first separation plates are integrally formed;

The side surface of the reaction liquid storage box is fixedly provided with a collision block, and the toothed plate pushes the reaction liquid storage box to transversely move when pushing the collision block on the reaction liquid storage box;

Through the technical scheme, when the toothed plate in the vibration arm assembly collides with the collision block on the reaction liquid storage box, the fourth spring is matched to stretch, so that the reaction liquid storage box transversely circulates and moves, the solution in a single inner cavity in the reaction liquid storage box is guaranteed to be fully mixed, when the inside of the reaction liquid storage box needs to be cleaned, the first partition plate and the second partition plate are pulled out upwards, then the controller controls the water pump to work so as to suck out waste liquid in the reaction liquid storage box, and accordingly the reaction liquid storage box is matched with the knocking of the vibration arm assembly to shake, and various solutions in the reaction liquid storage box are shaken.

Preferably, the liquid suction nozzle supply assembly comprises a bearing plate, lifting frames, a second supporting seat, first screw rods, unidirectional supporting pieces, a first motor and supporting columns, wherein four second supporting seats are arranged on the second supporting seat, the lower ends of the second supporting seats are respectively fixed on the inner bottom side of the base, the first motor is fixedly arranged at the lower ends of the second supporting seats, the output ends of the first motors are upwards fixedly provided with the first screw rods, the upper ends of the first screw rods are rotated at the upper ends of the second supporting seats, the lifting frames are two, the first screw rods in the two second supporting seats in the same direction penetrate through one lifting frame in a threaded manner, unidirectional supporting pieces are arranged on one side, close to each other, of the two lifting frames, two bearing plates are inserted between the two lifting frames in an up-down moving manner, disposable liquid suction nozzles are inserted in the bearing plates in a downward moving manner, the lower ends of the supporting columns are fixedly arranged on the inner bottom side of the base, and the upper ends of the supporting columns support the lowest bearing plates when the lifting frames move upwards;

The unidirectional support piece comprises a rotating plate and a lower support frame, the lower support frame is fixed at the upper end of the lifting frame, the rotating plate rotates in the upper end of the lower support frame through a rotating shaft, the lower side of the rotating plate is abutted against the upper side of the lower support frame, the width of the rotating plate is larger than that of the lower support frame, and one end of the rotating plate, which is positioned outside the lower support frame, is movably inserted below the bearing plate;

the uppermost bearing plate is knocked when the toothed plate moves out of the transverse moving seat;

according to the technical scheme, the controller controls the first motor to electrify, so that the first motor drives the first screw rod to rotate, the first screw rod drives the lifting frame to move up and down, when the lifting frame moves down, the rotating plate rotates upwards due to shielding of the bearing plate, the rotating plate skips over the upper bearing plate until the rotating plate moves below the upper bearing plate, the rotating plate rotates downwards below the bearing plate, at the moment, the lifting frame moves upwards, the lifting frame drives the lower support frame to move upwards, the lower support frame drives the rotating plate to move upwards, the rotating plate drives the upper bearing plate to move upwards, the upper bearing plate moves out of the base, the lower bearing plate moves upwards in a moving mode of the upper bearing plate, two layers of disposable liquid suction nozzles can be placed in at one time, one layer of disposable liquid suction nozzles can be lifted upwards, the two disposable liquid suction nozzles in the two bearing plates can be taken out by the clamping nozzle pipe, and the use is more convenient;

In addition, when the upper bearing plate moves upwards, the disposable liquid suction nozzle in the upper bearing plate is downwards inserted into the disposable liquid suction nozzle in the lower bearing plate, and the upper disposable liquid suction nozzle can be driven by friction force to move upwards by the lower disposable liquid suction nozzle, so that the toothed plate in the vibration arm assembly can strike the upper bearing plate to vibrate the upper bearing plate and the disposable liquid suction nozzle, and the upper disposable liquid suction nozzle vibrates relative to the lower disposable liquid suction nozzle to avoid adhesion between the upper disposable liquid suction nozzle and the lower disposable liquid suction nozzle.

Preferably, the mouth tube control assembly comprises a second lifting plate, an electric sliding table, a thread sleeve, a third lifting plate, a fourth screw rod and a fourth motor, wherein the fourth motor is fixed in a suspension arm, the output end of the fourth motor is fixedly provided with the fourth screw rod, the other end of the fourth screw rod rotates in the suspension arm, the external thread of the fourth screw rod is sleeved with two thread sleeves, the side surface of the thread sleeve is fixedly provided with the electric sliding table, the electric sliding table slides back and forth, the output ends of the two electric sliding tables are respectively fixedly provided with the second lifting plate and the third lifting plate, the two electric sliding tables respectively drive the second lifting plate and the third lifting plate to move up and down, a mouth clamping pipe penetrates through the second lifting plate, the linear array penetrates through the mouth clamping pipe in the third lifting plate, and the sum of the number of the mouth clamping pipes in the second lifting plate and the mouth clamping pipe in the third lifting plate is equal to the number of single-row disposable liquid suction nozzles in the receiving plate;

According to the technical scheme, the controller controls the fourth motor to be electrified to work, the fourth motor drives the fourth screw rod to rotate positively and negatively, the fourth screw rod drives the threaded sleeve to move forwards and backwards, the threaded sleeve drives the electric sliding table to move forwards and backwards, the electric sliding table drives the second lifting plate and the third lifting plate to move forwards and backwards, the second lifting plate and the third lifting plate drive the mouth clamping pipe to move forwards and backwards, when the mouth clamping pipe moves forwards, the mouth clamping pipe is aligned with the front group of pipe penetrating holes, and when the mouth clamping pipe moves backwards, the mouth clamping pipe is aligned with the rear group of pipe penetrating holes, so that the mouth clamping pipe can be respectively aligned with the front liquid suction nozzle supply assembly, the rear liquid suction nozzle supply assembly, the reaction liquid storage assembly, the blood coagulation plate assembly and the residual liquid storage box through controlling the forward and backward movement of the mouth clamping pipe, two groups of experiments can be alternately performed, and multiple test items can be alternately ensured;

The controller controls the two electric sliding tables to drive the second lifting plate and the third lifting plate to move up and down respectively, when the second lifting plate and the third lifting plate move down, the mouth clamping pipe moves down to be inserted into the disposable liquid suction nozzle in the bearing plate, when the second lifting plate and the third lifting plate move up, the mouth clamping pipe drives the disposable liquid suction nozzle to move up to pull out the disposable liquid suction nozzle from the bearing plate, and when the mouth clamping pipe moves up into the suspension arm completely, the lower side of the suspension arm pushes down the disposable liquid suction nozzle to separate the disposable liquid suction nozzle from the mouth clamping pipe.

Preferably, the lifting assembly comprises a second motor, four second screws and a first lifting plate, wherein the second motor is fixed in the equipment box, the second screws are fixed at the output end of the second motor, the upper ends of the second screws rotate at the upper end of the equipment box, the four second screws respectively penetrate through four corners of the first lifting plate in a threaded manner, the first lifting plate moves up and down in the equipment box, and the end parts of the first lifting plate are fixed on the side surfaces of the suspension arms;

through above-mentioned technical scheme, the second motor circular telegram work of controller control, second motor drive second screw rod positive and negative rotation, and the second screw rod drives the first lifter plate and reciprocates, and first lifter plate drives the davit and reciprocates to this drives the davit through lifting assembly and reciprocates.

Preferably, the second power assembly comprises a roller and a sixth motor, the sixth motor is fixed in the equipment box, the roller is fixed at the output end of the sixth motor, and the roller rolls on the second sliding rail;

through the technical scheme, the controller controls the sixth motor to work in a power-on mode, the sixth motor drives the idler wheels to rotate positively and negatively, the idler wheels roll on the second sliding rail, the equipment box moves along the second sliding rail, and the equipment box is driven to move transversely through the second power assembly.

The beneficial effects of the invention are as follows:

The mouth tube control assembly drives the mouth tube to move up and down, the mouth tube is inserted into the disposable liquid suction mouth in the liquid suction mouth supply assembly when moving downwards, and the disposable liquid suction mouth is taken out when the mouth tube moves upwards, so that the function of automatically taking and placing the disposable liquid suction mouth is realized;

When the disposable liquid suction nozzle is downwards inserted into the reaction liquid storage component, the pbs buffer liquid, the newcastle disease standard antigen, the unit virus or the chicken erythrocyte suspension in the reaction liquid storage component can be sucked, namely, four steps of manually sucking the pbs buffer liquid by a micropipette, sucking the newcastle disease standard antigen by the micropipette, sucking the unit virus by the micropipette and sucking the chicken erythrocyte suspension by the micropipette are replaced, so that the labor intensity of manual experiments is reduced;

When the disposable liquid suction nozzle is downwards inserted into the blood coagulation plate component, the vacuum pump works to enable positive pressure to be formed in the mouth clamping pipe and the disposable liquid suction nozzle, and a pbs buffer solution, a newcastle disease standard antigen, a unit virus or chicken erythrocyte suspension in the disposable liquid suction nozzle can be pushed out, namely, the step of manually dropping the solution into the blood coagulation plate is replaced, and when the disposable liquid suction nozzle is downwards inserted into or upwards pulled out of the blood coagulation plate component, the vacuum pump works to enable the mouth clamping pipe and the disposable liquid suction nozzle to circularly form positive pressure and negative pressure, and the pbs buffer solution, the newcastle disease standard antigen, the unit virus or chicken erythrocyte suspension in the blood coagulation plate can be circularly sucked, so that the purpose of performing multiple dilution on the solution in the blood coagulation plate is realized;

when the disposable liquid suction nozzle is positioned above the residual liquid storage box, the clamping nozzle pipe is completely retracted into the suspension arm, so that the disposable liquid suction nozzle is forcibly separated from the clamping nozzle pipe, the disposable liquid suction nozzle after use falls into the residual liquid storage box for temporary storage, and the waste solution in the disposable liquid suction nozzle falls into the residual liquid storage box for storage, thereby realizing the purpose of storing the waste disposable liquid suction nozzle and residual liquid;

The first power assembly drives the vibration arm assembly to move among the two liquid suction nozzle supply assemblies, the two reaction liquid storage assemblies and the two blood coagulation plate assemblies, and the vibration arm assembly can strike the reaction liquid storage assemblies and the blood coagulation plate assemblies, so that the solution in the reaction liquid storage assemblies and the blood coagulation plate assemblies is more uniformly mixed and reacted;

In conclusion, the device utilizes the second power component to drive the equipment box to move, the equipment box drives the suspension arm to transversely move, and the equipment box moves to the upper parts of the liquid suction nozzle supply component, the reaction liquid storage component, the blood coagulation plate component and the residual liquid storage box respectively, so that the purposes of automatically taking and placing the disposable liquid suction nozzle, automatically sucking solution, automatically discharging solution, automatically diluting solution and recycling the waste disposable liquid suction nozzle and residual liquid are respectively realized, when the vibration arm component knocks the vibration liquid suction nozzle supply component, the reaction liquid storage component and the blood coagulation plate component, the upper and lower layer disposable liquid suction nozzles can be separated and the reaction liquid can be rocked, the whole experimental process is automatically completed, the operation is convenient, the labor intensity of manual experiment is smaller,

Drawings

Fig. 1 is a schematic view of a partially sectioned perspective structure of the present invention.

Fig. 2 is a partially enlarged schematic view of the portion a of fig. 1.

Fig. 3 is a top view of the present invention.

Fig. 4 is a schematic cross-sectional view of the structure at B-B of fig. 3.

Fig. 5 is a schematic view of a cross-sectional structure at C-C of fig. 3.

FIG. 6 is a schematic perspective view, partially in section, of a platelet assembly of the present invention.

FIG. 7 is a schematic perspective view of a reaction solution storage assembly according to the present invention, partially in section.

Fig. 8 is a schematic perspective view of the first power assembly and the shock arm assembly of the present invention when mated.

FIG. 9 is a schematic perspective view of the invention with the combination of the platelet assembly, the reaction fluid reservoir assembly, the first power assembly and the vibration arm assembly.

FIG. 10 is a bottom view of the invention with the combination of the platelet assembly, the reactant reservoir assembly, the first power assembly, and the vibration arm assembly.

FIG. 11 is a side view of the invention showing the assembly of the hemagglutination plate, the reaction solution reservoir assembly, the first power assembly and the vibration arm assembly.

Fig. 12 is a perspective view of the nozzle supply assembly, the first power assembly and the vibration arm assembly of the present invention in combination.

Fig. 13 is a partially enlarged schematic view of the portion D of fig. 12.

In the figure: 1. an equipment box; 2. a wire receiving assembly; 201. a first spring; 202. a wire carrying frame; 203. a spring support rod; 204. a first slide rail; 3. a controller; 4. a residual liquid storage tank; 5. a hemagglutination plate assembly; 501. a blood coagulation plate; 502. a limit rod; 503. a second spring; 504. an inner support block; 505. a third spring; 506. a chassis; 507. a mounting column; 6. a reaction liquid storage assembly; 601. a first partition plate; 602. a second partition plate; 603. a reaction liquid storage tank; 604. a first support base; 605. a fourth spring; 606. a residual liquid discharge pipe; 607. a water pump; 7. a pipette tip supply assembly; 701. a receiving plate; 702. a lifting frame; 703. a second support base; 704. a first screw; 705. a unidirectional support; 7051. a rotating plate; 7052. a lower support frame; 706. a first motor; 707. a support column; 8. a base; 9. an upper plate; 10. a lower plate; 11. a vacuum pump; 12. a lifting assembly; 1201. a second motor; 1202. a second screw; 1203. a first lifting plate; 13. disposable liquid suction nozzle; 14. a mouth clamping pipe; 15. penetrating the pipe hole; 16. a suspension arm; 17. a mouthpiece control assembly; 1701. a second lifting plate; 1702. an electric sliding table; 17021. a third motor; 17022. a sliding frame; 17023. a third screw; 1703. a thread sleeve; 1704. a third lifting plate; 1705. a fourth screw; 1706. a fourth motor; 18. a first power assembly; 1801. a fifth motor; 1802. a fifth screw; 19. a second slide rail; 20. a second power assembly; 2001. a roller; 2002. a sixth motor; 21. a collision block; 22. a vibrating arm assembly; 2201. a toothed plate; 2202. a traversing seat; 2203. a gear; 2204. and a seventh motor.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to fig. 1 to 13. It should be understood by those skilled in the art that these embodiments are merely for explaining the technical principles of the present invention, and are not intended to limit the scope of the present invention.

As shown in fig. 1-5, 9 and 12, an automatic operation instrument for poultry hemagglutination antibody experiment comprises a base 8, a device box 1 and a suspension arm 16;

Base 8: the upper end of the base 8 is fixedly provided with a controller 3, the input end of the controller 3 is electrically connected with an external power supply, the upper end of the base 8 is fixedly provided with an upper layer plate 9 and a lower layer plate 10, the upper layer plate 9 is positioned above the lower layer plate 10, the upper layer plate 9 and the lower layer plate 10 are internally and sequentially provided with a liquid suction nozzle supply assembly 7, a reaction liquid storage assembly 6, a blood coagulation plate assembly 5 and a residual liquid storage box 4 along the length direction of the base 8, a disposable liquid suction nozzle 13 is inserted in a rectangular array in the liquid suction nozzle supply assembly 7, the reaction liquid storage assembly 6 and the residual liquid storage box 4 are two and are arranged side by side from front to back, the blood coagulation plate assembly 5 is four and is divided into two rows, the end, close to each other, of the front reaction liquid storage component 6 and the rear reaction liquid storage component 6 and the end, close to each other, of the front blood coagulation plate component 5 are both fixed with a collision block 21, the collision blocks 21 are staggered with each other, a first power component 18 is installed in the base 8, the output end of the first power component 18 is fixed with a vibration arm component 22, the first power component 18 drives the vibration arm component 22 to move between the front liquid suction nozzle supply component 7 and the rear liquid suction nozzle supply component 7, between the front reaction liquid storage component 6 and the rear reaction liquid storage component 6 and between the front blood coagulation plate component 5 and between the rear blood coagulation plate component 5, the vibration arm component 22 knocks the collision blocks 21 on the reaction liquid storage component 6 and the front blood coagulation plate component 5 and the rear blood coagulation plate component 5, the vibration arm component 22 knocks the liquid suction nozzle supply component 7, and a second slide rail 19 is fixed in the base 8 along the length direction;

Equipment box 1: the equipment box 1 moves on a second sliding rail 19, namely the equipment box 1 moves left and right along the length direction of the base 8, a second power assembly 20 is arranged between the equipment box 1 and the second sliding rail 19, the second power assembly 20 drives the equipment box 1 to move, a vacuum pump 11 is fixed in the equipment box 1, the output end of the controller 3 is electrically connected with the input end of the vacuum pump 11, and a lifting assembly 12 is arranged in the equipment box 1;

Boom 16: the boom 16 is fixed at the output end of the lifting assembly 12, the lifting assembly 12 drives the boom 16 to move up and down, the boom 16 moves above the liquid suction nozzle supply assembly 7, the reaction liquid storage assembly 6, the blood coagulation plate assembly 5 and the residual liquid storage box 4, front and back two groups of through holes 15 are formed in the lower side of the boom 16, each group of through holes 15 respectively pass through the upper parts of the liquid suction nozzle supply assembly 7, the reaction liquid storage assembly 6, the transverse two groups of blood coagulation plate assemblies 5 and the residual liquid storage box 4, the inside of each through hole 15 moves up and down to pass through a clamping nozzle pipe 14, the upper end of the clamping nozzle pipe 14 is communicated with the vacuum pump 11 through a soft plastic pipe, a disposable liquid suction nozzle 13 is movably sleeved at the lower end of the clamping nozzle pipe 14, the material of the clamping nozzle pipe 14 is made of hard plastic, the lower end of the clamping nozzle pipe 14 is clamped into the upper end of the disposable liquid suction nozzle 13 downwards, the outer diameter of the upper end of the disposable liquid suction nozzle 13 is larger than the diameter of each through hole 15, when the clamping nozzle 14 moves up, the disposable liquid suction nozzle 13 can not pass through the through holes 15 in a forced mode, the pipe 13 is forcibly, the upper end of the clamping nozzle 14 is controlled to move up and down, and the upper end of the clamping nozzle 14 is controlled to move down to move up and down, and the upper end of the clamping nozzle 14 is aligned with the upper end of the clamping nozzle 14 through the pipe 14, and the upper end of the pipe 14 is controlled to move forward and the pipe 17 when the clamping nozzle 17 is controlled to move up and the upper end of the clamping nozzle 14 is aligned with the upper end of the pipe 15.

As shown in fig. 1, fig. 4, fig. 12 and fig. 13, the first power assembly 18 includes a fifth motor 1801 and a fifth screw 1802, the fifth motor 1801 is fixed in the upper end of the base 8, the input end of the fifth motor 1801 is electrically connected with the output end of the controller 3, the output end of the fifth motor 1801 is fixed with the fifth screw 1802, the fifth screw 1802 rotates in the base 8, the inner side of the base 8 and the output end of the fifth motor 1801 support the fifth screw 1802 together, and the fifth screw 1802 is located between the front and rear liquid suction nozzle supply assemblies 7, between the front and rear reaction liquid storage assemblies 6 and between the front and rear blood coagulation plate assemblies 5;

The vibration arm assembly 22 comprises a toothed plate 2201, a transverse moving seat 2202, a gear 2203 and a seventh motor 2204, wherein a fifth screw 1802 thread penetrates through the transverse moving seat 2202, the middle of the lower layer plate 10 is hollow to form a track, the upper end of the transverse moving seat 2202 is movably inserted in the middle of the lower layer plate 10, so that the transverse moving seat 2202 moves left and right along the middle of the lower layer plate 10, namely the transverse moving seat 2202 moves along the length direction of the base 8, the side surface of the transverse moving seat 2202 is fixedly provided with the seventh motor 2204, the input end of the seventh motor 2204 is electrically connected with the output end of the controller 3, the output end of the seventh motor 2204 is fixedly provided with the gear 2203 after penetrating into the transverse moving seat 2202 in a rotating manner, the gear 2203 is meshed with the middle end teeth of the toothed plate 2201, and two ends of the toothed plate 2201 respectively movably penetrate out of two ends of the transverse moving seat 2202;

The movement steps of the first power assembly 18 and the vibration arm assembly 22 are: the controller 3 controls the fifth motor 1801 to be electrified to work, so that the fifth motor 1801 drives the fifth screw 1802 to rotate positively and negatively, the fifth screw 1802 drives the traversing seat 2202 to move left and right, the controller 3 controls the seventh motor 2204 to be electrified to work, so that the seventh motor 2204 drives the gear 2203 to rotate positively and negatively, the gear 2203 drives the toothed plate 2201 to move forwards and backwards through inter-tooth meshing, when the toothed plate 2201 moves forwards, the front liquid suction nozzle supply assembly 7, the front conflict block 21 on the reaction liquid storage assembly 6 and the front conflict block 21 on the blood coagulation plate assembly 5 are knocked, and when the toothed plate 2201 moves backwards, the rear liquid suction nozzle supply assembly 7, the rear conflict block 21 on the reaction liquid storage assembly 6 and the rear conflict block 21 on the blood coagulation plate assembly 5 are knocked.

As shown in fig. 4 and fig. 6, the blood coagulation plate assembly 5 comprises a blood coagulation plate 501, a limit rod 502, a second spring 503, an inner supporting block 504, a third spring 505, a chassis 506 and a mounting column 507, wherein long holes are formed in four corners of the chassis 506, the mounting column 507 is transversely movably inserted in the long holes, the upper end of the mounting column 507 supports the lower side of the chassis 506, the third spring 505 is fixed between the upper end of the mounting column 507 and the side surface of the long holes, the lower end of the mounting column 507 is fixed on the inner bottom side of the base 8, the limit rod 502 is transversely movably inserted in four corners of the upper end of the chassis 506, the moving directions of the limit rod 502 and the chassis 506 are the same, the other end of the limit rod 502 is fixedly provided with the inner supporting block 504, the second spring 503 is fixed between the inner supporting block 504 and the chassis 506, and the four inner supporting blocks 504 are vertically movably clamped in the lower end of the blood coagulation plate 501;

the side surface of the chassis 506 is fixed with a collision block 21, and the toothed plate 2201 pushes the chassis 506 to move transversely when pushing the collision block 21 on the chassis 506;

the movement steps of the blood coagulation plate assembly 5 are: the blood coagulation plate 501 is downwards sleeved outside the four inner supporting blocks 504, the second springs 503 push the inner supporting blocks 504, so that the four inner supporting blocks 504 clamp the blood coagulation plate 501, when the toothed plate 2201 in the vibration arm assembly 22 knocks against the supporting blocks 21 on the chassis 506, the chassis 506 moves transversely, the chassis 506 drives the inner supporting blocks 504 to move transversely through the second springs 503, and the inner supporting blocks 504 drive the blood coagulation plate 501 to move transversely, so that the solution in the blood coagulation plate 501 is fully mixed.

As shown in fig. 4 and fig. 7, the reaction solution storage assembly 6 includes a first partition plate 601, a second partition plate 602, a reaction solution storage tank 603, a first support seat 604, a fourth spring 605, a residual solution discharge pipe 606 and a water pump 607, wherein two first support seats 604 are respectively fixed at the inner lower side of the base 8 by screws, the reaction solution storage tank 603 is transversely and movably inserted between the upper ends of the two first support seats 604, the fourth spring 605 is fixed between the reaction solution storage tank 603 and the first support seats 604, the lower side of the reaction solution storage tank 603 passes through the residual solution discharge pipe 606, the end part of the residual solution discharge pipe 606 is fixed at the water inlet end of the water pump 607, the input end of the water pump 607 is electrically connected with the output end of the controller 3, the water outlet end of the water pump 607 is connected with an external waste liquid collecting tank, the second partition plate 602 and the two first partition plates 601 are inserted in the reaction solution storage tank 603 in an up-down movable manner, the two first partition plates 601 are integrally formed, the two first partition plates 601 are perpendicular to the second partition plate 602, and the two first partition plates 602 and the two first partition plates 601 divide the reaction solution storage tank 603 into four cavities;

The side surface of the reaction liquid storage box 603 is fixed with a collision block 21, and when the toothed plate 2201 pushes the collision block 21 on the reaction liquid storage box 603, the reaction liquid storage box 603 is pushed to move transversely;

The movement steps of the reaction liquid storage component 6 are as follows: when the toothed plate 2201 in the vibration arm assembly 22 hits the abutting block 21 on the reaction liquid storage box 603, the fourth spring 605 is matched to stretch and retract, so that the reaction liquid storage box 603 transversely and circularly moves, the solution in a single inner cavity in the reaction liquid storage box 603 is guaranteed to be fully mixed, when the inside of the reaction liquid storage box 603 needs to be cleaned, the first partition plate 601 and the second partition plate 602 are pulled out upwards, and then the controller 3 controls the water pump 607 to work so as to suck out waste liquid in the reaction liquid storage box 603.

As shown in fig. 4, 12 and 13, the nozzle feeding assembly 7 includes a receiving plate 701, a lifting frame 702, a second supporting seat 703, a first screw 704, a unidirectional supporting member 705, a first motor 706 and a supporting column 707, the second supporting seat 703 has four lower ends and is fixed at the inner bottom side of the base 8, the first motor 706 is fixed at the lower end of the second supporting seat 703, the input end of the first motor 706 is electrically connected with the output end of the controller 3, the output end of the first motor 706 is upwardly fixed with the first screw 704, the upper end of the first screw 704 is rotated at the upper end of the second supporting seat 703, so that the output end of the first motor 706 and the upper end of the second supporting seat 703 together support the first screw 704, the two lifting frames 702 are arranged, first screw rods 704 in two second supporting seats 703 in the same direction penetrate through one lifting frame 702 in a threaded manner, one-way supporting pieces 705 are arranged on one side, close to each other, of the two lifting frames 702, two bearing plates 701 are movably inserted between the two lifting frames 702 up and down, disposable liquid suction nozzles 13 are movably inserted in the bearing plates 701 down, the disposable liquid suction nozzles 13 are in a rectangular array, the one-way supporting pieces 705 push the bearing plates 701 to move up when the lifting frames 702 move up, the lower ends of the supporting columns 707 are fixed on the inner bottom side of the base 8, and the upper ends of the supporting columns 707 support the lowest bearing plates 701;

The unidirectional support 705 comprises a rotating plate 7051 and a lower support frame 7052, the lower support frame 7052 is fixed at the upper end of the lifting frame 702, the rotating plate 7051 rotates in the upper end of the lower support frame 7052 through a rotating shaft, the lower side of the rotating plate 7051 abuts against the upper side of the lower support frame 7052, the width of the rotating plate 7051 is larger than that of the lower support frame 7052, and one end of the rotating plate 7051, which is positioned outside the lower support frame 7052, is movably inserted into the lower part of the bearing plate 701;

Striking the uppermost receiving plate 701 when the toothed plate 2201 moves out of the traversing seat 2202;

The movement steps of the mouthpiece supply assembly 7 are: the controller 3 controls the first motor 706 to be electrified, so that the first motor 706 drives the first screw 704 to rotate, the first screw 704 drives the lifting frame 702 to move up and down, when the lifting frame 702 moves down, the rotating plate 7051 rotates upwards due to shielding of the bearing plate 701, so that the rotating plate 7051 skips over the upper bearing plate 701 until the rotating plate 7051 moves below the upper bearing plate 701, the rotating plate 7051 rotates downwards below the bearing plate 701, at the moment, the lifting frame 702 moves upwards, the lifting frame 702 drives the lower supporting frame 7052 to move upwards, the lower supporting frame 7052 drives the rotating plate 7051 to move upwards, the rotating plate 7051 drives the upper bearing plate 701 to move upwards, the upper bearing plate 701 moves out of the base 8, and the upward movement mode of the lower bearing plate 701 is performed according to the movement mode of the upper bearing plate 701;

In addition, when the upper receiving plate 701 moves upwards, since the disposable pipette tip 13 in the upper receiving plate 701 is inserted downwards into the disposable pipette tip 13 in the lower receiving plate 701, the upper disposable pipette tip 13 may be moved upwards by friction force to drive the lower disposable pipette tip 13, so that the toothed plate 2201 in the vibration arm assembly 22 may strike the upper receiving plate 701 to vibrate the upper receiving plate 701 and the disposable pipette tip 13, and the upper disposable pipette tip 13 vibrates relative to the lower disposable pipette tip 13 to avoid adhesion between the upper disposable pipette tip 13 and the lower disposable pipette tip 13.

As shown in fig. 1, fig. 4 and fig. 5, the nozzle control assembly 17 comprises a second lifting plate 1701, an electric sliding table 1702, a threaded sleeve 1703, a third lifting plate 1704, a fourth screw 1705 and a fourth motor 1706, wherein the fourth motor 1706 is fixed in the suspension arm 16, the input end of the fourth motor 1706 is electrically connected with the output end of the controller 3, the output end of the fourth motor 1706 is fixed with the fourth screw 1705, the other end of the fourth screw 1705 rotates in the suspension arm 16, the external thread of the fourth screw 1705 is sleeved with two threaded sleeves 1703, the side surface of the threaded sleeve 1703 is fixed with the electric sliding table 1702, the input end of the electric sliding table 1702 is electrically connected with the output end of the controller 3, the output ends of the electric sliding table 1702 are respectively fixed with the second lifting plate 1701 and the third lifting plate 1704, the two electric sliding tables 1702 respectively drive the second lifting plate 1701 and the third lifting plate 1704 to move up and down, the second lifting plate 1701 passes through a nozzle pipe 14, the number of the third lifting plate 1701 passes through the nozzle pipe 14 in a straight line and the nozzle array 14 in the third lifting plate, and the number of the nozzle pipe 1704 is equal to the number of the nozzles 13 in the single nozzle pipe 1701;

the movement mode of the mouth tube control assembly 17 is as follows: the controller 3 controls the fourth motor 1706 to be electrified to work, the fourth motor 1706 drives the fourth screw 1705 to rotate positively and negatively, the fourth screw 1705 drives the threaded sleeve 1703 to move forwards and backwards, the threaded sleeve 1703 drives the electric sliding table 1702 to move forwards and backwards, the electric sliding table 1702 drives the second lifting plate 1701 and the third lifting plate 1704 to move forwards and backwards, the second lifting plate 1701 and the third lifting plate 1704 drive the mouth clamping pipe 14 to move forwards and backwards, when the mouth clamping pipe 14 moves forwards, the mouth clamping pipe 14 is aligned with the front group of through pipe holes 15, and when the mouth clamping pipe 14 moves backwards, the mouth clamping pipe 14 is aligned with the rear group of through pipe holes 15;

The controller 3 controls the two electric sliding tables 1702 to drive the second lifting plate 1701 and the third lifting plate 1704 to move up and down respectively, when the second lifting plate 1701 and the third lifting plate 1704 move down, the nozzle clamping tube 14 moves down to be inserted into the disposable nozzle 13 in the bearing plate 701, when the second lifting plate 1701 and the third lifting plate 1704 move up, the nozzle clamping tube 14 drives the disposable nozzle 13 to move up to pull the disposable nozzle 13 out of the bearing plate 701, and when the nozzle clamping tube 14 moves up completely into the suspension arm 16, the lower side of the suspension arm 16 pushes down the disposable nozzle 13 to separate the disposable nozzle 13 from the nozzle clamping tube 14.

As shown in fig. 1 and fig. 4, the electric sliding table 1702 includes a third motor 17021, a sliding frame 17022 and a third screw rod 17023, the sliding frame 17022 is inserted into the boom 16 in a front-back movable manner, the upper end of the sliding frame 17022 is fixed with the third motor 17021, the input end of the third motor 17021 is electrically connected with the output end of the controller 3, the output end of the third motor 17021 is fixed with the third screw rod 17023 after penetrating into the sliding frame 17022 in a rotating manner, the lower end of the third screw rod 17023 is rotated at the lower end of the sliding frame 17022, and the third screw rods 17023 in the two electric sliding tables 1702 respectively pass through the second lifting plate 1701 and the third lifting plate 1704 in a threaded manner.

As shown in fig. 1 and fig. 5, the lifting assembly 12 includes a second motor 1201, a second screw 1202 and a first lifting plate 1203, the second motor 1201 is four and is fixed in the equipment box 1, the input end of the second motor 1201 is electrically connected with the output end of the controller 3, the output end of the second motor 1201 is fixed with the second screw 1202, the upper end of the second screw 1202 rotates at the upper end of the equipment box 1, the four second screws 1202 respectively pass through four corners of the first lifting plate 1203 in a threaded manner, the first lifting plate 1203 moves up and down in the equipment box 1, and the end part of the first lifting plate 1203 is fixed at the side surface of the boom 16;

The lifting assembly 12 moves in the following manner: the controller 3 controls the second motor 1201 to be electrified, the second motor 1201 drives the second screw 1202 to rotate positively and negatively, the second screw 1202 drives the first lifting plate 1203 to move up and down, and the first lifting plate 1203 drives the boom 16 to move up and down.

As shown in fig. 5, the second power assembly 20 includes a roller 2001 and a sixth motor 2002, the sixth motor 2002 is fixed in the equipment box 1, an input end of the sixth motor 2002 is electrically connected with an output end of the controller 3, the roller 2001 is fixed at an output end of the sixth motor 2002, and the roller 2001 rolls on the second sliding rail 19;

The movement mode of the second power assembly 20 is as follows: the controller 3 controls the sixth motor 2002 to be electrified, the sixth motor 2002 drives the roller 2001 to rotate positively and negatively, and the roller 2001 rolls on the second sliding rail 19, so that the equipment box 1 moves along the second sliding rail 19.

As shown in fig. 1 and fig. 2, a wire storage assembly 2 is installed in the rear end of a base 8, the wire storage assembly 2 comprises a first spring 201, a wire carrying frame 202, a spring supporting rod 203 and a first sliding rail 204, the first sliding rail 204 is fixed in the rear end of the base 8 along the length direction of the base 8, the first sliding rail 204 slides with at least 2 wire carrying frames 202, a first spring 201 is fixed between two adjacent wire carrying frames 202, the side surface of each wire carrying frame 202 is fixed with the spring supporting rod 203, the spring supporting rod 203 is positioned in the first spring 201, a through hole is arranged in each wire carrying frame 202, and the spring supporting rod 203 on each wire carrying frame 202 transversely moves through the through hole in each adjacent wire carrying frame 202;

The movement steps of the electric wire containing assembly 2 are as follows: wires between the controller 3 and the vacuum pump 11, wires between the controller 3 and the second motor 1201, wires between the controller 3 and the third motor 17021, wires between the controller 3 and the fourth motor 1706, wires between the controller 3 and the sixth motor 2002, and wires between the controller 3 and the seventh motor 2204 are laid and fixed on the wire carrying frame 202 to prevent the wires from being tangled, when the apparatus box 1 moves rightward, the left end of the wires moves rightward, the distance between the two wire carrying frames 202 becomes small, the first spring 201 is compressed, when the apparatus box 1 moves leftward, the left end of the wires moves leftward, the first spring 201 stretches, and the distance between the two wire carrying frames 202 becomes large, thereby receiving the wires at the moment to prevent the wires from being tangled.

The whole motion mode of the device is as follows:

The second power assembly 20 drives the equipment box 1 to move left and right, the equipment box 1 drives the suspension arm 16 to move transversely, so that the suspension arm 16 can move above the liquid suction nozzle supply assembly 7, the reaction liquid storage assembly 6, the blood coagulation plate assembly 5 and the residual liquid storage box 4 respectively, when the equipment box moves to a designated position, the lifting assembly 12 drives the suspension arm 16 to move up and down, and meanwhile, the mouth pipe control assembly 17 drives the mouth pipe 14 to move up and down;

When the boom 16 moves above the pipette tip supply assembly 7, the disposable pipette tip 13 in the pipette tip supply assembly 7 is inserted when the pipette tip 14 moves downward, and the disposable pipette tip 13 is taken out when the pipette tip 14 moves upward;

When the suspension arm 16 moves to the upper part of the reaction liquid storage component 6, the disposable liquid suction nozzle 13 is downwards inserted into each inner cavity in the reaction liquid storage box 603, the vacuum pump 11 works to enable the clamping nozzle pipe 14 and the disposable liquid suction nozzle 13 to form negative pressure, and the abs buffer solution, newcastle disease standard antigen, unit virus or chicken erythrocyte suspension of the reaction liquid storage box 603 can be sucked in batches;

When the suspension arm 16 moves to the upper part of the blood coagulation plate assembly 5, the disposable liquid suction nozzle 13 is inserted downwards into the blood coagulation plate 501 of the blood coagulation plate assembly 5, the vacuum pump 11 works to enable the inside of the clamping nozzle pipe 14 and the disposable liquid suction nozzle 13 to form positive pressure, and the abs buffer solution, newcastle disease standard antigen, unit virus or chicken erythrocyte suspension in the disposable liquid suction nozzle can be pushed out;

Similarly, when the disposable liquid suction nozzle 13 is inserted downwards or pulled upwards into the hemagglutination plate 501, the vacuum pump 11 works to enable the inside of the clamping nozzle pipe 14 and the disposable liquid suction nozzle 13 to form positive pressure and negative pressure, so that the pbs buffer solution, newcastle disease standard antigen, unit virus or chicken erythrocyte suspension in the hemagglutination plate can be sucked and discharged circularly, and the purpose of carrying out double dilution on the solution in the hemagglutination plate 501 is realized;

When the boom 16 moves to the upper part of the residual liquid storage box 4, the clamping nozzle pipe 14 is completely retracted into the boom 16, so that the disposable liquid suction nozzle 13 is forcibly separated from the clamping nozzle pipe 14, the disposable liquid suction nozzle 13 after use falls into the residual liquid storage box 4 for temporary storage, and the waste solution in the disposable liquid suction nozzle 13 falls into the residual liquid storage box 4 for storage.

It should be noted that, in the description of the present invention, terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like, which indicate directions or positional relationships, are based on the directions or positional relationships shown in the drawings, are merely for convenience of description, and do not indicate or imply that the apparatus or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Furthermore, it should be noted that, in the description of the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those skilled in the art according to the specific circumstances.

Thus far, the technical solution of the present invention has been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of protection of the present invention is not limited to these specific embodiments. Equivalent modifications and substitutions for related technical features may be made by those skilled in the art without departing from the principles of the present invention, and such modifications and substitutions will fall within the scope of the present invention.

Claims (8)

1. An automatic operation instrument for poultry hemagglutination antibody experiments is characterized by comprising a base (8), an equipment box (1) and a suspension arm (16);

Base (8): the utility model discloses a blood coagulation device, including base (8) and base, wherein imbibition mouth supply assembly (7), reaction liquid storage assembly (6), blood coagulation plate assembly (5) and raffinate storage box (4) are installed in proper order along its length direction in the upper end of base (8), rectangular array alternates in imbibition mouth supply assembly (7) has disposable imbibition mouth (13), imbibition mouth supply assembly (7), reaction liquid storage assembly (6) and raffinate storage box (4) all have two and back side by side, blood coagulation plate assembly (5) have four and divide into two rows, the one end that two reaction liquid storage assembly (6) are close to each other and the one end that two blood coagulation plate assembly (5) are close to each other all are fixed with conflict piece (21) and conflict piece (21) are misplaced each other, install first power component (18) in base (8), the output of first power component (18) is fixed with vibrations arm assembly (22), first power component (18) drive vibrations arm assembly (22) and are supplied with two liquid absorption mouth assemblies (7) and two back side by side after and two reaction liquid storage assembly (6) and shake between two preceding blood coagulation plate assembly (5) and between two reaction liquid storage assembly (7) and two preceding vibration arm assembly (22) are beaten in advance between two blood coagulation plate assembly (5), a second sliding rail (19) is fixed in the base (8) along the length direction;

Equipment box (1): the equipment box (1) moves on a second sliding rail (19), a second power assembly (20) is arranged between the equipment box (1) and the second sliding rail (19), the second power assembly (20) drives the equipment box (1) to move, a vacuum pump (11) is fixed in the equipment box (1), and a lifting assembly (12) is arranged in the equipment box (1);

Boom (16): the lifting device is characterized in that the lifting arm (16) is fixed at the output end of the lifting assembly (12), the lifting assembly (12) drives the lifting arm (16) to move up and down, the lifting arm (16) moves above the liquid suction nozzle supply assembly (7), the reaction liquid storage assembly (6), the blood coagulation plate assembly (5) and the residual liquid storage box (4), two groups of pipe penetrating holes (15) are formed in the lower side of the lifting arm (16), each group of pipe penetrating holes (15) respectively pass through the upper parts of the liquid suction nozzle supply assembly (7), the reaction liquid storage assembly (6), the two groups of blood coagulation plate assemblies (5) and the residual liquid storage box (4), a clamping nozzle pipe (14) is movably arranged in the pipe penetrating holes (15) up and down, the upper end of the clamping nozzle pipe (14) is communicated with a vacuum pump (11) through a plastic pipe, the lower end of the clamping nozzle pipe (14) is movably sleeved with a disposable liquid suction nozzle (13), the outer diameter of the upper end of the disposable liquid suction nozzle (13) is larger than the diameter of the pipe penetrating hole (15), the lifting arm (16) is internally provided with a control assembly (17), and the clamping nozzle (14) is fixedly arranged at the upper end of the lifting arm (14) and the upper end of the clamping nozzle (17).

2. The automatic poultry hemagglutination antibody experimental operation instrument according to claim 1, wherein the first power component (18) comprises a fifth motor (1801) and a fifth screw rod (1802), the fifth motor (1801) is fixed in the upper end of the base (8), the output end of the fifth motor (1801) is fixed with the fifth screw rod (1802), the fifth screw rod (1802) rotates in the base (8), and the fifth screw rod (1802) is positioned between the front and rear liquid suction nozzle supply components (7), between the front and rear reaction liquid storage components (6) and between the front and rear hemagglutination plate components (5);

the vibration arm assembly (22) comprises a toothed plate (2201), a transverse moving seat (2202), a gear (2203) and a seventh motor (2204), wherein threads of a fifth screw (1802) penetrate through the transverse moving seat (2202), the transverse moving seat (2202) moves along the length direction of a base (8), the seventh motor (2204) is fixed on the side face of the transverse moving seat (2202), the gear (2203) is fixed in the transverse moving seat (2202) after the output end of the seventh motor (2204) rotates and penetrates into the transverse moving seat (2202), the gear (2203) is meshed with middle-end teeth of the toothed plate (2201), and two ends of the toothed plate (2201) respectively movably penetrate out of two ends of the transverse moving seat (2202).

3. The automatic poultry hemagglutination antibody experimental operation instrument according to claim 2, wherein the hemagglutination plate component (5) comprises a hemagglutination plate (501), a limit rod (502), a second spring (503), an inner supporting block (504), a third spring (505), a chassis (506) and a mounting column (507), wherein long strip holes are respectively arranged at four corners of the chassis (506) and are transversely movably penetrated with the mounting column (507), the upper end of the mounting column (507) supports the chassis (506), the third spring (505) is fixed between the upper end of the mounting column (507) and the side surface of the long strip holes, the lower end of the mounting column (507) is fixed at the inner bottom side of the base (8), the limit rod (502) is transversely movably penetrated at four corners of the upper end of the chassis (506), the movement direction of the limit rod (502) is the same as that of the chassis (506), the inner supporting block (504) is fixed at the other end of the limit rod (502), the second spring (504) is fixed between the inner supporting block (504) and the chassis (506), and the upper end of the four inner supporting blocks (503) are movably penetrated in the base (8);

The side of chassis (506) is fixed with conflict piece (21), when the conflict piece (21) on chassis (506) is moved in pinion rack (2201) top, promote chassis (506) lateral shifting.

4. The automatic poultry hemagglutination antibody experimental operation instrument according to claim 2, wherein the reaction solution storage component (6) comprises a first partition plate (601), a second partition plate (602), a reaction solution storage box (603), a first support seat (604), a fourth spring (605), a residual solution discharge pipe (606) and a water pump (607), wherein the two first support seats (604) are respectively fixed at the inner lower side of the base (8), the reaction solution storage box (603) is transversely and movably inserted between the upper ends of the two first support seats (604), a fourth spring (605) is fixed between the reaction solution storage box (603) and the first support seat (604), the lower side of the reaction solution storage box (603) is penetrated by the residual solution discharge pipe (606), the end part of the residual solution discharge pipe (606) is fixed with a water pump (607), the second partition plate (602) and the two first partition plates (601) are inserted in the reaction solution storage box (603) in an up-down movable manner, and the second partition plate (602) and the two first partition plates (601) are integrally formed;

The side of reaction liquid storage box (603) is fixed with conflict piece (21), pinion rack (2201) pushes reaction liquid storage box (603) lateral shifting when pushing conflict piece (21) on reaction liquid storage box (603).

5. The automated avian hemagglutination antibody assay operating apparatus according to claim 2, wherein the pipette tip supply assembly (7) comprises a receiving plate (701), a lifting frame (702), a second support base (703), a first screw (704), a unidirectional support (705), a first motor (706) and a support column (707), wherein the second support base (703) has four lower ends and is fixed at the inner bottom side of the base (8), the first motor (706) is fixed at the lower end of the second support base (703), the first screw (704) is fixed at the output end of the first motor (706) upwards, the upper end of the first screw rod (704) rotates at the upper end of the second supporting seat (703), the two lifting frames (702) are arranged, the first screw rods (704) in the two second supporting seats (703) in the same direction are threaded through one lifting frame (702), one side, close to each other, of the two lifting frames (702) is provided with a one-way supporting piece (705), two bearing plates (701) are inserted between the two lifting frames (702) in an up-down movable way, disposable liquid suction nozzles (13) are inserted in the bearing plates (701) in a downward movable way, the disposable liquid suction nozzles (13) are in a rectangular array, the one-way supporting piece (705) pushes the bearing plates (701) to move upwards when the lifting frames (702) move upwards, the lower ends of the support columns (707) are fixed on the inner bottom side of the base (8), and the upper ends of the support columns (707) support the lowest bearing plate (701);

The unidirectional support (705) comprises a rotating plate (7051) and a lower support frame (7052), the lower support frame (7052) is fixed at the upper end of the lifting frame (702), the rotating plate (7051) rotates in the upper end of the lower support frame (7052) through a rotating shaft, the lower side of the rotating plate (7051) is abutted against the upper side of the lower support frame (7052), the width of the rotating plate (7051) is larger than that of the lower support frame (7052), and one end of the rotating plate (7051) outside the lower support frame (7052) is movably inserted into the lower part of the bearing plate (701);

the toothed plate (2201) strikes the uppermost bearing plate (701) when moving out of the traversing seat (2202).

6. The automated avian hemagglutination antibody assay instrument of claim 5, wherein the nozzle control assembly (17) comprises a second lifter plate (1701), an electric sliding table (1702), a threaded sleeve (1703), a third lifter plate (1704), a fourth screw (1705) and a fourth motor (1706), wherein the fourth motor (1706) is fixed in the boom (16), the fourth screw (1705) is fixed at the output end of the fourth motor (1706), the other end of the fourth screw (1705) is rotated in the boom (16), two threaded sleeves (1703) are externally threaded on the fourth screw (1705), the side surface of the threaded sleeve (1703) is fixed with the electric sliding table (1702), the electric sliding table (1702) slides back and forth, the output ends of the two electric sliding tables (1702) are respectively fixed with the second lifter plate (1701) and the third lifter plate (1704), the two electric sliding tables (1702) respectively drive the second lifter plate (1701) and the third lifter plate (1705) to move up and down, the second lifter plate (1701) passes through the inner nozzle (14) and the inner nozzle (14), the sum of the number of the clamping mouth pipes (14) in the second lifting plate (1701) and the number of the clamping mouth pipes (14) in the third lifting plate (1704) is equal to the number of the single-row disposable liquid suction nozzles (13) in the bearing plate (701).

7. The automatic poultry hemagglutination antibody experimental operation instrument according to claim 1, wherein the lifting assembly (12) comprises a second motor (1201), a second screw rod (1202) and a first lifting plate (1203), the second motor (1201) is four and is fixed in the equipment box (1), the second screw rod (1202) is fixed at the output end of the second motor (1201), the upper end of the second screw rod (1202) rotates at the upper end of the equipment box (1), the four second screw rods (1202) respectively penetrate through four corners of the first lifting plate (1203) in a threaded manner, the first lifting plate (1203) moves up and down in the equipment box (1), and the end part of the first lifting plate (1203) is fixed on the side surface of the suspension arm (16).

8. An automated avian hemagglutination antibody assay instrument according to claim 1, wherein the second power assembly (20) comprises a roller (2001) and a sixth motor (2002), wherein the sixth motor (2002) is fixed within the device box (1), wherein the roller (2001) is fixed to the output of the sixth motor (2002), and wherein the roller (2001) rolls on the second rail (19).