CN118330059A - Method for simultaneously measuring contents of 6 volatile components in jasmine flower liquid by adopting rapid gas chromatography - Google Patents
Method for simultaneously measuring contents of 6 volatile components in jasmine flower liquid by adopting rapid gas chromatography Download PDFInfo
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Abstract
The invention discloses a method for simultaneously measuring the content of 6 volatile components in jasmine flower liquid by adopting a rapid gas chromatography, wherein in the method, a sample is diluted by absolute ethyl alcohol and is fixed to a scale, a 0.45 mu m filter membrane is used for filtration, a gas chromatograph is adopted for measurement, a capillary chromatographic column DB-WAX is selected as an analysis column, N 2 is used as carrier gas, the flow is 2.0 mL/min, the split ratio is 10:1, the sample injection volume is 1 mu L, the temperature of a sample injection port is 250 ℃, a detector is a hydrogen Flame Ionization Detector (FID), the temperature of the detector is 260 ℃, the temperature is programmed, and the external standard method is used for quantification. The method is simple and convenient to operate, quick and accurate and reliable in result, can be used for quickly and accurately measuring the contents of 6 characteristic aroma volatile components of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole in the jasmine liquid, can be used for quality control and evaluation of the jasmine liquid, and is beneficial to quality control of the jasmine liquid.
Description
Technical Field
The invention relates to the technical field of chemical detection, in particular to a method for simultaneously measuring the contents of 6 volatile components of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole in jasmine flower liquid by adopting a rapid gas chromatography method.
Background
Jasmine flower (school name: jasminum sambac (l.) Aiton) is a plant of the genus jasminum of the family luteaceae. The jasmine flower has elegant fragrance, and is fragrant and overflowed, contains volatile components such as linalool, phyllitol acetate, methyl benzoate, benzyl acetate, cis-3-hexenyl benzoate, alpha-farnesene, methyl anthranilate, indole and the like, and is an important spice and also a raw material for manufacturing jasmine tea. The jasmine flower is more precious in essential oil extracted by taking the jasmine flower as a raw material except for scenting tea leaves. The jasmine essential oil has low yield, elegant smell and various biological activities, has the effects of relieving depressed emotion, stimulating spirit, improving self-confidence, nursing and improving dry skin, water deficiency, oil passing and sensitive conditions, fading striae of pregnancy, scars and the like.
The traditional extraction method of jasmine essential oil mainly comprises an adsorption method, a steam distillation method, a reduced pressure steam distillation method, an organic solvent extraction method, a supercritical extraction method and a subcritical extraction method. Along with the development of industrial technology, some enterprises develop a new extraction method of jasmine essential oil, and the oil-water mixture rich in jasmine volatile components is obtained by utilizing the steps of cultivating flowers, extracting air containing the jasmine volatile components and condensing, wherein the upper layer is the jasmine essential oil after separation, and the lower layer is aromatic water solution containing the jasmine aromatic gas component (the application is called jasmine solution). The jasmine flower liquid is as follows: when the jasmine essential oil is extracted, the jasmine is subjected to flower cultivation, air containing volatile components of the jasmine is extracted, and condensed to obtain an oil-water mixture, and the aromatic water solution containing the jasmine aroma components is obtained after the jasmine essential oil is separated by oil-water separation; or extracting flos Jasmini sambac essential oil by steam distillation, condensing, oil-water separating, and collecting the aqueous solution containing flos Jasmini sambac aroma component. The jasmine flower liquid contains jasmine flower fragrance components, is mild in property as compared with essential oil, does not irritate skin, and can be used for skin care, such as cosmetic water, preparation mask and the like; in order to further develop the jasmine flower liquid and improve the added value, the inventor analyzes the volatile components of the jasmine flower liquid, and discovers that the main volatile components are phyllitol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole.
The content and variety of volatile components of jasmine are influenced by factors such as variety, production place, environment, climate conditions and the like, and the release of aroma components is influenced by factors such as physiological and biochemical reactions of the jasmine, so that in order to further control the quality of the jasmine liquid and provide basis for optimizing the production process, it is necessary to establish a method for measuring the content of various aroma components in the jasmine liquid. At present, related research reports on qualitative analysis and content measurement of aroma components in jasmine flower and jasmine essential oil and jasmine absolute oil exist, but the content measurement of volatile components in jasmine liquid is hardly reported. The application discloses a high performance liquid chromatography method for simultaneously measuring 5 aroma components in jasmine flower hydrolat, which can simultaneously measure the contents of 5 aroma components of benzyl alcohol, methyl anthranilate, indole, benzyl acetate and linalool in the jasmine flower hydrolat, but has long analysis and measurement time. Therefore, the method which is simpler, more convenient, quicker, shorter in measurement time, accurate in result and suitable for measuring the content of various volatile components in the jasmine flower liquid is further developed, and the quality of the jasmine flower liquid is better controlled.
Disclosure of Invention
The invention aims to provide a method for simultaneously measuring the contents of 6 volatile components in jasmine flower liquid by adopting a rapid gas chromatography, which is accurate and reliable, simple and convenient to operate and short in measuring time, can rapidly and accurately measure the contents of 6 volatile components in the jasmine flower liquid, namely, leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole, can be used for quality control and evaluation of the jasmine flower liquid, and provides accurate and reliable scientific basis for the establishment of quality standards of the jasmine flower liquid.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for simultaneously measuring the contents of 6 volatile components in jasmine flower liquid by adopting a rapid gas chromatography method comprises the following steps of:
(1) Chromatographic conditions
Chromatographic column: DB-WAX chromatographic column [10 m X0.18 mm X0.18 μm ], sample inlet temperature: 250. DEG C, detector: FID, detector temperature: 260. c, sample injection mode: split sample introduction, wherein the split ratio is 10:1; sample injection amount: 1 μl; carrier gas species: high-purity nitrogen; carrier gas flow rate: 2mL/min; programming temperature: the initial temperature is 80 ℃, the temperature is kept for 1 min, and the temperature is increased to 130 ℃ at 23 ℃/min; 20. raising the temperature to 220 ℃ per min; 80. raising the temperature to 250 ℃ per min, and keeping the temperature at 1 min;
(2) Solution preparation
Standard stock solution: respectively weighing appropriate amounts of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole standard substances, precisely weighing, dissolving with absolute ethyl alcohol, diluting, and fixing volume to prepare standard stock solution; the mass concentrations of the leaf alcohol, the linalool, the benzyl acetate, the benzyl alcohol, the methyl anthranilate and the indole in the standard stock solution are 0.9325 mg/mL, 0.9640 mg/mL, 1.067 mg/mL, 1.049 mg/mL, 1.185 mg/mL and 1.014 mg/mL respectively;
Series of mixed standard working solutions: respectively precisely absorbing a proper amount of standard stock solution, and gradually diluting with absolute ethyl alcohol to prepare a series of mixed standard working solutions;
(3) Preparation of sample solution: accurately sucking jasmine flower liquid 2 mL-10 mL in a volumetric flask, diluting with absolute ethanol, fixing volume to scale, mixing, filtering with 0.45 μm microporous membrane, and measuring with gas chromatograph;
(4) And (3) measuring: respectively taking a sample solution and a series of mixed standard working solutions, injecting the sample solutions into a gas chromatograph, carrying out sample injection measurement according to the chromatographic conditions, and quantitatively calculating the content of 6 volatile components in a jasmine flower liquid sample by adopting an external standard curve method;
The jasmine flower liquid is as follows: when the jasmine essential oil is extracted, the jasmine is subjected to flower cultivation, air containing volatile components of the jasmine is extracted, and condensed to obtain an oil-water mixture rich in the volatile components of the jasmine, and the aromatic water solution containing the jasmine aroma components is obtained after the oil-water separation and the separation of the jasmine essential oil; or extracting flos Jasmini sambac essential oil by steam distillation, condensing, oil-water separating, and collecting the aqueous solution containing flos Jasmini sambac aroma component.
In the method for simultaneously measuring the contents of 6 volatile components in the jasmine flower liquid by adopting the rapid gas chromatography, the external standard curve method is adopted for quantification, wherein the standard curve is drawn: and (3) injecting a series of mixed standard working solutions into a gas chromatograph, carrying out sample injection measurement according to the chromatographic conditions, recording the chromatographic peak area of each volatile component, carrying out linear regression by taking the mass concentration of the solution as an abscissa and the peak area as an ordinate, and drawing a standard curve to obtain a linear regression equation.
The beneficial effects of the invention are as follows:
The invention adopts a rapid gas chromatography to establish a method for simultaneously measuring the contents of 6 volatile components of phyllitol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole in jasmine flower liquid. The method has the advantages of simple and rapid operation, short measurement time, accurate and reliable result, good precision and stability, good separation degree among all target components, good linear relation between 6 target objects and chromatographic peak area in the corresponding mass concentration range, and high practicability. The method can be used for rapidly and accurately measuring the contents of 6 characteristic aroma volatile components of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole in the jasmine liquid, can be used for quality control and evaluation of the jasmine liquid, is favorable for quality control of the jasmine liquid, provides a basis for optimization of the production process of the jasmine liquid, and provides an accurate and reliable scientific basis for establishment of the quality standard of the jasmine liquid. Compared with the method (Zhang Ronglin, lv Min, xue Yaxin, and the like) established before the inventor, the method disclosed by the invention has the advantages that the analysis time is saved, the method is simpler and quicker, the measurement time is short, meanwhile, the gas chromatography uses nitrogen as carrier gas, no organic reagent is needed, and the method meets the requirements of green chemistry.
Drawings
FIG. 1 is a gas chromatogram of a mixed standard working solution, 1-leaf alcohol, 2-linalool, benzyl 3-acetate, 4-benzyl alcohol; 5-methyl anthranilate; 6-indole;
FIG. 2 is a gas chromatogram of a sample solution of jasmine flower liquid, 1-leaf alcohol; 2-linalool; 3-benzyl acetate; 4-benzyl alcohol; 5-methyl anthranilate; 6-indole.
Detailed Description
Example 1
1. Experimental part
1.1 Main instrument and reagent
Gas chromatograph: GC-2010Plus type, equipped with Labsolution workstation, hydrogen Flame Ionization Detector (FID), shimadzu corporation. An electronic balance: XP-26, feeling of 0.001 mg, switzerland Metrele Toli.
Leaf alcohol standard: the purity (mass fraction) was 98.6%, dr. Linalool standard: the purity (mass fraction) was 97.8%, beijing Man Ha Ge Biotechnology Co. Benzyl alcohol standard: the purity (mass fraction) was 99.9%, dr. Benzyl acetate standard: the purity (mass fraction) was 99.4%, dr. Methyl anthranilate standard: the purity (mass fraction) was 99.9%, company CATO in U.S. Indole standard: the purity (mass fraction) was 99.5%, company CATO in U.S. Absolute ethyl alcohol: chromatographic purity, merck company, germany.
Jasmine flower liquid sample: the method is provided by the biological technology limited company of Ding Tianxiang in Guangxi Guangzhou city, and is characterized in that when jasmine essential oil is extracted, the jasmine is subjected to flower culture, air containing volatile components of the jasmine is extracted, and condensed to obtain an oil-water mixture rich in the volatile components of the jasmine, and the aromatic water solution containing the jasmine aroma components is obtained after the oil-water separation and the separation of the jasmine essential oil.
Laboratory water was prepared from Millipore pure water.
1.2 Chromatographic conditions
Chromatographic column: DB-WAX column [10 m X0.18 mm X0.18 μm, agilent company, U.S. ]; sample inlet temperature: 250. the temperature is lower than the temperature; a detector: FID; detector temperature: 260. the temperature is lower than the temperature; sample injection mode: split sample introduction, wherein the split ratio is 10:1; sample injection amount: 1 mul. Carrier gas species: high-purity nitrogen; carrier gas flow rate: 2mL/min; programming temperature: the initial temperature is 80 ℃, the temperature is kept for 1 min, and the temperature is increased to 130 ℃ at 23 ℃/min; 20. raising the temperature to 220 ℃ per min; 80. the temperature is raised to 250 ℃ per minute, and the temperature is kept at 1 min.
1.3 Solution preparation
1.3.1 Standard stock solution
Respectively weighing appropriate amounts of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole standard substances, precisely weighing, dissolving with absolute ethyl alcohol, diluting, and fixing volume to prepare standard stock solution. The mass concentrations of the leaf alcohol, the linalool, the benzyl acetate, the benzyl alcohol, the methyl anthranilate and the indole in the standard stock solution are 0.9325 mg/mL, 0.9640 mg/mL, 1.067 mg/mL, 1.049 mg/mL, 1.185 mg/mL and 1.014 mg/mL respectively.
1.3.2 Series of mixed standard working solutions
Respectively precisely sucking a proper amount of standard stock solution, and gradually diluting with absolute ethyl alcohol to prepare a series of mixed standard working solutions, wherein the specific concentration is shown in table 1.
1.3.3 Preparation of sample solutions
Accurately sucking jasmine liquid 2 mL-10 mL in a volumetric flask, diluting with absolute ethanol, fixing volume to scale, mixing, filtering with 0.45 μm microporous membrane, and measuring with gas chromatograph.
2. Results and analysis
2.1 Optimization of chromatographic conditions and sampling volume
The peak pattern of the sample solution on the HP-5 and DB-WAX capillary chromatographic columns was compared. The results show that linalool and phyllitol peak tailing on the HP-5 capillary chromatography column and improved peak profile when using the WAX column. The conventional gas chromatography analysis uses a multi-purpose 30m capillary chromatographic column, the analysis time is long, and in order to save the analysis time and improve the analysis efficiency, the invention selects a 10m capillary chromatographic column for analysis, and simultaneously optimizes the temperature rise program. Under the temperature programming condition, the analysis time is shortened, and the peak types of the chromatographic peaks of 6 target components are symmetrical and all reach baseline separation.
The jasmine flower liquid is an aqueous solution, so that a direct dilution method is adopted to extract a sample. The excessive water content of the sample can affect the chromatographic peak type of the analytical component, and the long-time use of the sample can cause the reduction of the column efficiency of the chromatographic column. The influence of different sampling amounts on the peak type was examined by using absolute ethyl alcohol as a solvent, and when the sampling amount was more than 5.00mL, the tailing of the chromatographic peak was serious, and when the sampling amount was 2.00 mL, the peak type of all components was optimal, so that the sampling amount was determined to be 2.00 mL.
2.2 Chromatographic separation effect
And respectively precisely sucking the mixed standard working solution under the item "1.3.2" and the sample solution under the item "1.3.3", and carrying out sample injection measurement according to the chromatographic condition under the item "1.2". The retention times of the leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole are respectively 1.565, 2.225, 2.925, 3.545, 4.924 and 5.692 min, the chromatographic peak shapes are good, and the chromatograms are shown in figures 1-2.
2.3 Linear relation, detection limit and quantitative limit
Taking a series of mixed standard working solutions under the item "1.3.2", carrying out sample injection measurement according to chromatographic conditions under the item "1.2", recording the chromatographic peak areas of all components, carrying out linear regression by taking the mass concentration (x, mug/mL) of the solution as an abscissa and the peak area (y) as an ordinate, and calculating a regression equation and a correlation coefficient. The mixed standard working solution was diluted step by step, the mass concentration of the target peak 3 times of the signal to noise ratio was taken as a detection limit, and the mass concentration of the target peak 10 times of the signal to noise ratio was taken as a quantitative limit, and the results are shown in Table 2.
2.4 Precision test
Taking the same batch of jasmine flower liquid samples, preparing 6 parts of sample solution according to the method under the item of 1.3.2, carrying out sample injection measurement according to the chromatographic condition under the item of 1.2, and calculating the content of 6 characteristic aroma components, wherein the measurement result is shown in Table 3. The relative standard deviations of the target contents were 0.85%, 1.27%, 1.13%, 1.46%, 1.20% and 1.30%, respectively, indicating that the method was excellent in precision.
2.5 Stability test
The sample solution under item "1.3.2" was measured under the chromatographic conditions under item "1.2" at intervals of 0, 5, 10, 16, 20, and 24 h, and the chromatographic peak areas of 6 characteristic aroma components were recorded, and the results are shown in Table 4. The relative standard deviations of the target chromatographic peak area measurements were 0.83%, 1.07%, 1.31%, 1.40%, 1.48%, 1.94%, respectively, indicating good stability of the sample solution within 24 h.
2.6 Label recovery test
9 Parts of a sample (number S1) with known content is precisely sucked, each part is 2.00 mL parts and placed in a 10mL volumetric flask, mixed standard solutions (the mass concentration of the leaf alcohol is 0.2803 mg/mL, the mass concentration of the linalool is 1.138 mg/mL, the mass concentration of the benzyl acetate is 1.136 mg/mL, the mass concentration of the benzyl alcohol is 0.9821 mg/mL, the mass concentration of the methyl anthranilate is 0.2108 mg/mL, the mass concentration of the indole is 0.2217 mg/mL) are respectively added into the mixed standard solutions, 100, 200 and 400 mu L of the mixed standard solutions are fixed to volume to marked lines by absolute ethyl alcohol, shaking is carried out, and the low, medium and high 3 concentration levels of standard recovery solutions are obtained, and sample feeding measurement is carried out according to the chromatographic condition under the item "1.2", and the results are shown in Table 5. As shown in Table 5, the method has the advantages that the labeling recovery rate of 6 target objects is 93.96% -110.45%, the accuracy is good, and the quantitative analysis requirement is met.
3. Idioms of the knot
The method for measuring the contents of 6 characteristic aroma components of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole in jasmine flower liquid is established by adopting a rapid gas chromatography. Compared with the method (Zhang Ronglin, lv Min, xue Yaxin, and the like) for simultaneously measuring 5 aroma components in the jasmine pure dew by using the high-performance liquid chromatography established before the inventor, the method saves analysis time, uses nitrogen as carrier gas by using the gas chromatography without using an organic reagent, and meets the requirement of green chemistry better. The method is accurate and reliable, and can rapidly and accurately detect the content of 6 characteristic aroma components in the jasmine flower liquid.
Example 2
A method for simultaneously measuring the contents of 6 volatile components of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole in jasmine flower liquid by adopting a rapid gas chromatography method specifically comprises the following steps:
(1) Chromatographic conditions
Chromatographic column: DB-WAX chromatographic column [10 m X0.18 mm X0.18 μm ], sample inlet temperature: 250. DEG C, detector: FID, detector temperature: 260. c, sample injection mode: split sample introduction, wherein the split ratio is 10:1; sample injection amount: 1 μl; carrier gas species: high-purity nitrogen; carrier gas flow rate: 2mL/min; programming temperature: the initial temperature is 80 ℃, the temperature is kept for 1 min, and the temperature is increased to 130 ℃ at 23 ℃/min; 20. raising the temperature to 220 ℃ per min; 80. raising the temperature to 250 ℃ per min, and keeping the temperature at 1 min;
(2) Solution preparation
Standard stock solution: respectively weighing appropriate amounts of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole standard substances, precisely weighing, dissolving with absolute ethyl alcohol, diluting, and fixing volume to prepare standard stock solution; the mass concentrations of the leaf alcohol, the linalool, the benzyl acetate, the benzyl alcohol, the methyl anthranilate and the indole in the standard stock solution are 0.9325 mg/mL, 0.9640 mg/mL, 1.067 mg/mL, 1.049 mg/mL, 1.185 mg/mL and 1.014 mg/mL respectively;
Series of mixed standard working solutions: respectively precisely absorbing a proper amount of standard stock solution, and gradually diluting with absolute ethyl alcohol to prepare a series of mixed standard working solutions;
(3) Preparation of sample solution: accurately sucking jasmine flower liquid 2 mL-10 mL in a volumetric flask, diluting with absolute ethanol, fixing volume to scale, mixing, filtering with 0.45 μm microporous membrane, and measuring with gas chromatograph;
(4) And (3) measuring: respectively taking a sample solution and a series of mixed standard working solutions, injecting the sample solutions into a gas chromatograph, carrying out sample injection measurement according to the chromatographic conditions, and quantitatively calculating the content of 6 volatile components in a jasmine flower liquid sample by adopting an external standard curve method; wherein the standard curve is drawn: taking a series of mixed standard working solutions, injecting the mixed standard working solutions into a gas chromatograph, carrying out sample injection measurement according to the chromatographic conditions, recording the chromatographic peak areas of all volatile components, carrying out linear regression by taking the mass concentration of the solutions as an abscissa and the peak areas as an ordinate, and drawing a standard curve to obtain a linear regression equation;
The jasmine flower liquid is as follows: when the jasmine essential oil is extracted, the jasmine is subjected to flower cultivation, air containing volatile components of the jasmine is extracted, and condensed to obtain an oil-water mixture rich in the volatile components of the jasmine, and the aromatic water solution containing the jasmine aroma components is obtained after the oil-water separation and the separation of the jasmine essential oil; or extracting flos Jasmini sambac essential oil by steam distillation, condensing, oil-water separating, and collecting the aqueous solution containing flos Jasmini sambac aroma component.
Claims (2)
1. A method for simultaneously measuring the contents of 6 volatile components in jasmine flower liquid by adopting a rapid gas chromatography is characterized by simultaneously measuring the contents of 6 volatile components of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole in the jasmine flower liquid by adopting the rapid gas chromatography, and specifically comprises the following steps:
(1) Chromatographic conditions
Chromatographic column: DB-WAX chromatographic column, sample inlet temperature: 250 ℃, detector: FID, detector temperature: 260 ℃, sample injection mode: split sample introduction, wherein the split ratio is 10:1; sample injection amount: 1 μl; carrier gas species: high-purity nitrogen; carrier gas flow rate: 2mL/min; programming temperature: the initial temperature is 80 ℃, the temperature is kept for 1 min, and the temperature is increased to 130 ℃ at 23 ℃/min; 20. raising the temperature to 220 ℃ per min; raising the temperature to 250 ℃ at 80 ℃/min, and keeping the temperature at 1 min;
(2) Solution preparation
Standard stock solution: respectively weighing appropriate amounts of leaf alcohol, linalool, benzyl acetate, benzyl alcohol, methyl anthranilate and indole standard substances, precisely weighing, dissolving with absolute ethyl alcohol, diluting, and fixing volume to prepare standard stock solution; the mass concentrations of the leaf alcohol, the linalool, the benzyl acetate, the benzyl alcohol, the methyl anthranilate and the indole in the standard stock solution are 0.9325 mg/mL, 0.9640 mg/mL, 1.067 mg/mL, 1.049 mg/mL, 1.185 mg/mL and 1.014 mg/mL respectively;
Series of mixed standard working solutions: respectively precisely absorbing a proper amount of standard stock solution, and gradually diluting with absolute ethyl alcohol to prepare a series of mixed standard working solutions;
(3) Preparation of sample solution: accurately sucking jasmine flower liquid 2 mL-10 mL in a volumetric flask, diluting with absolute ethanol, fixing volume to scale, mixing, filtering with 0.45 μm microporous membrane, and measuring with gas chromatograph;
(4) And (3) measuring: respectively taking a sample solution and a series of mixed standard working solutions, injecting the sample solutions into a gas chromatograph, carrying out sample injection measurement according to the chromatographic conditions, and quantitatively calculating the content of 6 volatile components in a jasmine flower liquid sample by adopting an external standard curve method;
The jasmine flower liquid is as follows: when the jasmine essential oil is extracted, the jasmine is subjected to flower cultivation, air containing volatile components of the jasmine is extracted, and condensed to obtain an oil-water mixture, and the aromatic water solution containing the jasmine aroma components is obtained after the jasmine essential oil is separated by oil-water separation; or extracting flos Jasmini sambac essential oil by steam distillation, condensing, oil-water separating, and collecting the aqueous solution containing flos Jasmini sambac aroma component.
2. The method for simultaneously determining the contents of 6 volatile components in jasmine flower liquid by adopting a rapid gas chromatography method according to claim 1, wherein the determination is carried out by adopting an external standard curve method, wherein a standard curve is drawn: and (3) injecting a series of mixed standard working solutions into a gas chromatograph, carrying out sample injection measurement according to the chromatographic conditions, recording the chromatographic peak area of each volatile component, carrying out linear regression by taking the mass concentration of the solution as an abscissa and the peak area as an ordinate, and drawing a standard curve to obtain a linear regression equation.
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