CN114034797A - Method for measuring content of flower components of dendrobium nobile lindl - Google Patents

Method for measuring content of flower components of dendrobium nobile lindl Download PDF

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CN114034797A
CN114034797A CN202111361335.2A CN202111361335A CN114034797A CN 114034797 A CN114034797 A CN 114034797A CN 202111361335 A CN202111361335 A CN 202111361335A CN 114034797 A CN114034797 A CN 114034797A
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段灿灿
田鑫
张建永
李尧
冯文忠
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Zunyi Medical University
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Abstract

The invention discloses a method for measuring the content of dendrobium nobile flower components in red water, which comprises the following steps: s1, preparation of a reference substance solution: precisely weighing liquiritin, kaempferol-3-glucose rhamnoside and isoquercitrin standard product respectively, and dissolving in solvent to obtain mixed reference solution; s2, preparing a test solution of the dendrobium nobile flowers in the red water; and S3, measuring the content by adopting a high performance liquid chromatography. The determination method determines the content of 3 chemical components, namely liquiritin, kaempferol-3-glucose rhamnoside and isoquercitrin, in the dendrobium nobile flower, and can better control the quality of the dendrobium nobile flower.

Description

Method for measuring content of flower components of dendrobium nobile lindl
Technical Field
The invention relates to the technical field of pharmacy, in particular to a method for measuring the content of dendrobium nobile flower components in red water.
Background
Herba Dendrobii belonging to the genus Dendrobium of OrchidaceaeDendrobium nobileLindl.) is one of 3 main varieties collected in dendrobium nobile in the 2020 edition of chinese pharmacopoeia. The Shennong herbal classic records that dendrobium stem is mainly used for treating injuries, removing arthralgia, descending qi, tonifying internal organs, wasting and strengthening yin. It can be taken for a long time to nourish intestines and stomach. Reducing weight and prolonging life. "think it is beneficial to stomach and promote the production of body fluidAnd nourishing yin and clearing heat.
Dendrobium nobile is mainly distributed in Guizhou, Sichuan, Yunnan, Guangxi, Hainan and Taiwan in China, and the best produced by Guizhou red water is the genuine medicinal material. The general survey results of Chinese medicinal material planting resources show that only Guizhou red water accords with the wild planting conditions of dendrobium nobile at the present stage in China, and the dendrobium nobile is also the main production area of dendrobium nobile in China.
At present, the main application part of dendrobium nobile lindl is stem, but the development of flowers and other parts is insufficient, a large amount of resources are wasted in an idle mode, and the development of the dendrobium nobile lindl industry is hindered. The dendrobium nobile flower is mostly used as tea drink and is listed as the Guizhou food local standard, DBS 52/049-. The standard comprises physicochemical detection of properties, sensory requirements, pollutant limit, moisture, gray and the like, and the content of dendrobine in the dendrobium candidum is not less than 0.4 percent. However, the research on the specific chemical components contained in the dendrobium stem flowers is less at present, the quality control method only has a fingerprint patent at present, only one component of D-anhydrous glucose is recognized, the characteristic is not strong, the whole is not yet complete, and the deep research is necessary. Therefore, the invention develops and establishes a detection method which is simple, convenient and easy to implement, has high accuracy and good repeatability and can reflect various chemical components contained in the dendrobium nobile flowers as much as possible.
Disclosure of Invention
The invention aims to overcome the difficulties in the background technology and provides a method for measuring the content of the flower components of dendrobium nobile lindl.
The three chemical components of the invention are: liquiritin, kaempferol-3-glucose rhamnoside, and isoquercitrin.
In order to achieve the purpose, the technical scheme is as follows: the invention discloses a method for measuring the content of dendrobium nobile flower components, which comprises the following steps:
s1, preparation of a reference substance solution:
precisely weighing liquiritin, kaempferol-3-glucose rhamnoside and isoquercitrin standard product respectively, and dissolving in solvent to obtain mixed reference solution;
s2, preparing a red water dendrobium stem flower test sample solution:
accurately weighing dried dendrobium nobile flowers in a conical flask, adding a proper amount of 70% methanol, weighing, shaking up, performing ultrasonic treatment, cooling to room temperature, then weighing again, complementing lost mass with 70% methanol, shaking up, filtering, and taking a subsequent filtrate to obtain a test sample solution;
s3, content determination:
respectively measuring chromatograms of the reference solution and the test solution by high performance liquid chromatography under the same detection conditions; and calculating the content of the component corresponding to the reference solution in the red water dendrobium nobile test solution according to the concentration of the reference solution, the peak area of the reference solution in the chromatogram and the peak area of the component corresponding to the reference solution in the test solution in the chromatogram.
Further, in the step S1, an appropriate amount of each standard substance is precisely weighed and respectively placed in a 5 mL volumetric flask, the volume is fixed to a scale with 70% methanol, and the mixture is ultrasonically oscillated and shaken up to prepare a mixed reference substance solution containing liquiritin 0.042 mg/mL, kaempferol-3-glucose rhamnoside 0.132 mg/mL and isoquercitrin 0.040 mg/mL.
Further, in the step S2, accurately weighing 0.5 g of dried dendrobium nobile flowers in a conical flask, adding 35 mL of 70% methanol, weighing, shaking up, performing ultrasonic treatment for 60 min (45 kHz, 90%), cooling to room temperature, weighing again, complementing lost mass with 70% methanol, shaking up, filtering, and taking a subsequent filtrate to obtain a sample solution.
Further, in step S3, the detecting condition includes: chromatography column Agilent Eclipse Plus C18 (4.6 mm x 150 mm, 5 μm); the flow rate is 1.0 mL/min; the detection wavelength is 280 nm; the column temperature is 30 ℃; gradient elution was performed with 0.06% acetic acid-water solution as mobile phase a and acetonitrile as mobile phase B.
Further, in step S3, during the gradient elution, the changes of the mobile phase a and the mobile phase B are: according to the volume fraction, the time is 0-4 min, 95% A → 87.5A; 4-14 min, 87.5% A → 83% A; 14-21 min, 83% A → 73% A; 21-25 min, 73% A → 0% A.
The determination method comprises the following steps: sucking the mixed reference solution and the test solution, injecting the mixed reference solution and the test solution into a high performance liquid chromatograph to obtain a chromatogram, and calculating the content of chemical components in the test solution according to the chromatogram;
chromatographic conditions are as follows: chromatography column Agilent Eclipse Plus C18(4.6 mm. times.150 mm, 5 μm); the flow rate is 1.0 mL/min; the column temperature is 30 ℃; gradient elution was performed with 0.06% acetic acid-water solution as mobile phase a and acetonitrile as mobile phase B.
The gradient elution procedure was as follows:
Figure 315609DEST_PATH_IMAGE001
the detection wavelength was 280 nm.
Further, in the step S3, the content measurement is performed by precisely measuring the mixed control solution 1, 2, 5, 10, 15, 20 μ L prepared in the step S1, injecting into a high performance liquid chromatograph, and measuring; and drawing a standard curve by taking the sample amount as a horizontal coordinate (X) and the peak area integral value as a vertical coordinate (Y), and calculating a regression equation, a linear range and a regression coefficient of each mixed reference substance.
Further, in the step S3, in the precision test of the content measuring instrument, the mixed reference solution prepared in the step S1 is continuously injected for 6 times under the chromatographic condition, 3 reference peak areas are measured, and the retention time and the RSD value of the peak area are calculated respectively.
Further, in the step S3, in the content determination repeatability test, 6 parts of the same red water dendrobium stem flower sample is taken to prepare a test sample solution, sample injection is performed under the same chromatographic condition, and the retention time and the RSD value of the peak area are calculated respectively.
Further, in the step S3, in the content determination stability test, the same red water dendrobium stem flower sample is taken, the sample solution is prepared according to the method in the step S2, sample injection is performed for 0 hour, 4 hours, 8 hours, 12 hours, 16 hours, 20 hours and 24 hours under the same chromatographic condition, the peak areas of 3 reference samples are measured, and the retention time and the RSD value of the peak area are calculated respectively.
Further, in the content measurement sample application recovery rate test in step S3, the same sample powder of which the content is known is precisely weighed to be 0.25 g, 9 parts in total, the mixed reference solution is precisely added according to the ratio of 1:0.8, 1:1, and 1:1.2, the sample solution is prepared according to the sample solution preparation method in step S2, the sample solution is measured 3 times under the chromatographic condition, the peak area of each component is recorded, and the recovery rate is calculated.
The invention has the beneficial effects that: the method for determining the content of 3 chemical components in the red water dendrobium nobile flowers by using the high performance liquid chromatography, which is established by the invention, meets the requirement of methodology verification, is simple and convenient to operate, high in sensitivity, accurate in determination and strong in applicability, and can be used for quality control of the product. The quality condition of the product can be reflected more comprehensively, and the quality stability of the product is ensured.
Drawings
FIG. 1 shows a chromatogram of a sample solution of Dendrobium nobile.
FIG. 2, mixed control solution chromatogram.
In the figure, 1, liquiritin; 2. kaempferol-3-glucose rhamnoside; 3. isoquercitrin.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to the specific embodiments of the present invention. The described embodiments are only some, not all embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 HPLC content determination method of Dendrobium Nobile flower
1 materials and methods
1.1 instruments
Agilent 1260 Infinity high performance liquid chromatograph equipped with G1315D variable wavelength detector (Agilent Corp., USA); KQ-300VDE model double frequency digital control ultrasonic cleaner (Kunshan ultrasonic instruments Co., Ltd.); waterpu ultrapure water plant (Watter Water treatment plant, Sichuan); SpectraMax Plus 384 microplate reader (milar instruments ltd); one-tenth-ten-thousandth electronic balance (sidoris scientific instruments ltd); electronic balance (shanghai cyanine sea instruments ltd); constant temperature water bath (hang zhou youning instruments limited).
1.2 reagent
Rutin (mass percent > 98%, batch No. AF 9022006), liquiritin (mass percent > 98%, batch No. AF 8111195), kaempferol-3-glucose rhamnoside (mass percent > 98%, batch No. AF 20050831), all purchased from Chengdou method biotechnology limited company; isoquercitrin (mass fraction > 98%, batch No. Y-076-180517) was purchased from Douglas Biotech Ltd.
The red water dendrobium flower sample was collected from Xintiankaikuan base, lot number 200505.
1.3 reagents
Methanol (chromatographically pure, alatin reagent), acetonitrile (chromatographically pure, alatin reagent), methanol (analytically pure, chengdu jinshan chemical reagent ltd), ethanol (analytically pure, chongqing wan-dong chemical reagent ltd), acetic acid (analytically pure, chengdu jinshan chemical reagent ltd).
1.4 methods
1.4.1 chromatographic conditions
The chromatography column Agilent Eclipse Plus C is adopted18(4.6 mm. times.150 mm, 5 μm); the flow rate is 1.0 mL/min; the column temperature is 30 ℃; the wavelength is 280 nm; the influence of 0.06% acetic acid-water solution as a mobile phase A and acetonitrile as a mobile phase B on the separation of each component of the red water dendrobium nobile flower is examined under different proportions of the system. The final gradient elution procedure was determined as shown in table 1 below.
TABLE 1 mobile phase gradient elution conditions
Figure 415152DEST_PATH_IMAGE002
1.4.2 preparation of control solutions
Taking a proper amount of each standard substance, precisely weighing, respectively placing in a 5 mL volumetric flask, fixing the volume to the scale with 70% methanol, ultrasonically oscillating, shaking up, and preparing into a mixed reference substance solution containing liquiritin 0.042 mg/mL, kaempferol-3-glucose rhamnoside 0.132 mg/mL, and isoquercitrin 0.040 mg/mL.
1.4.3 preparation of test solutions
Accurately weighing 0.5 g of dried dendrobium nobile flower in an erlenmeyer flask, adding 35 mL of 70% methanol, weighing, shaking uniformly, performing ultrasonic treatment for 60 min (45 kHz, 90%), cooling to room temperature, weighing again, compensating lost mass with 70% methanol, shaking uniformly, filtering, and taking a subsequent filtrate to obtain a sample solution.
2 methodology study
2.1 Linear relationship investigation
Precisely measuring 1, 2, 5, 10, 15, and 20 μ L of the mixed reference solution under 1.4.2, injecting into high performance liquid chromatograph, and measuring. Taking the sample amount as a horizontal coordinate (X) and the peak area integral value as a vertical coordinate (Y), drawing a standard curve, calculating a regression equation, a linear range and a regression coefficient of each mixed reference substance, and displaying that the linear relation of each component in a certain concentration range is good by the result shown in Table 2.
TABLE 2 Linear regression equation and correlation coefficient for three control samples
Figure 631370DEST_PATH_IMAGE003
2.2 precision test
Mixing the reference substance solution under item 1.4.2, continuously injecting sample for 6 times under chromatographic condition, measuring the peak areas of 3 reference substances, and respectively calculating the retention time and RSD value of the peak area. As a result, the RSD values of the retention times of the liquiritin, the kaempferol-3-glucose rhamnoside and the isoquercitrin are respectively as follows: 0.240%, 0.253%, and 0.241%; the RSD values of the peak areas were: 1.808%, 0.529% and 2.978%. Indicating that the precision of the instrument is good.
2.3 repeatability test
Taking 6 parts of the same red water dendrobium stem flower sample, preparing a sample solution, injecting the sample under the same chromatographic condition, and respectively calculating the retention time and the RSD value of the peak area. As a result, the RSD values of the retention times of the liquiritin, the kaempferol-3-glucose rhamnoside and the isoquercitrin are respectively as follows: 0.220%, 0.249% and 0.239%; the RSD values of the corresponding component contents of the peak areas are respectively as follows: 3.747%, 3.599% and 1.907%. Indicating that the method has good repeatability.
2.4 stability test
Taking the same red water dendrobium stem flower sample, preparing a sample solution according to the method under '1.4.3', respectively injecting samples for 0, 4, 8, 12, 16, 20 and 24 hours under the same chromatographic condition, measuring the peak areas of 3 reference samples, and respectively calculating the RSD values of retention time and peak areas. As a result, the RSD values of the retention times of the liquiritin, the kaempferol-3-glucose rhamnoside and the isoquercitrin are respectively as follows: 0.294%, 0.263% and 0.434%; the RSD values of the peak areas were: 2.845%, 2.898% and 4.530%. The detection quality of the test solution is stable within 24 h.
2.5 sample application recovery test
Precisely weighing 0.25 g of the same sample powder with known content, precisely adding 9 parts of the powder into a mixed reference solution according to the proportion of 1:0.8, 1:1 and 1:1.2 respectively, preparing a test solution according to the method under item 1.4.3, measuring 3 times under chromatographic conditions respectively, recording the peak area of each component, and calculating the recovery rate. Recovery = (C-a)/B × 100%, where a is the amount of the component to be measured contained in the sample, B is the amount of the added control, and C is the measured value. The results are shown in Table 3, indicating that the process yields are good.
TABLE 3 results of sample recovery rate of 3 chemical components in Dendrobium Nobile flower
Figure 844701DEST_PATH_IMAGE004
Systematic methodology research shows that the linear relation of 3 compounds is good, and the method has good precision, repeatability and stability, meets the requirements of related methodologies, and can be used for content determination of samples.
Example 2 optimization of chromatography conditions and sample treatment method for Dendrobium Nobile flower
Agilent Eclipse Plus C was investigated in the experiment18Column (150 mm. times.4.6 mm, 5 μm), Thermo AcclaimTM 120 C18Column (150 mm. times.4.6 mm, 5 μm) and Agilent Eclipse XDB-C18Three chromatographic columns (150 mm × 4.6 mm, 5 μm) and three detection wavelengths of 254, 280 and 360 nm, the result isIndicating that Agilent Eclipse Plus C18The column (150 mm. times.4.6 mm, 5 μm) was more efficient and the sample solution had a better peak shape at 280 nm. In addition, different mobile phase systems such as acetic acid water-methanol, acetic acid water-acetonitrile, phosphoric acid water-methanol, phosphoric acid water-acetonitrile and the like are investigated in the experiment, and the result shows that the peak emergence time is most suitable under the 0.06 percent acetic acid water-acetonitrile mobile phase system, the characteristic peak can meet the requirement of the separation degree, the base line of the chromatogram is relatively stable, and the peak symmetry is good.
Under the same conditions, the extraction of 40%, 70%, 100% ethanol samples was examined first in this experiment, and the optimum extraction solvent percentage was selected according to the peak shape and peak area. The percentage of methanol is used again to compare the result with the percentage of ethanol. Finally, when 70% methanol is used as an extraction solvent, the peak shape of the sample is the best, and the total area of the characteristic peaks is the largest.
Under the condition of the same other conditions, the extraction conditions of the samples after 30 min, 60 min and 90 min of ultrasound are considered in the experiment, and the total area of the characteristic peaks of the samples is the largest when the samples are extracted for 60 min.
Under the condition that other conditions are the same, the extraction conditions of samples subjected to ultrasonic extraction, cold soaking and water bath reflux at 65 ℃ for 60 min are respectively considered in experiments, the total area of characteristic peaks of the samples subjected to water bath reflux extraction is only slightly higher than that of the samples subjected to ultrasonic extraction, and the ultrasonic extraction is selected as an optimal method in the aspects of easy operation, efficiency and the like.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (10)

1. The method for measuring the content of the components of the dendrobium nobile lindl flower is characterized by comprising the following steps:
s1, preparation of a reference substance solution:
precisely weighing liquiritin, kaempferol-3-glucose rhamnoside and isoquercitrin standard product respectively, and dissolving in solvent to obtain mixed reference solution;
s2, preparing a red water dendrobium stem flower test sample solution:
accurately weighing dried dendrobium nobile flowers in a conical flask, adding a proper amount of 70% methanol, weighing, shaking up, performing ultrasonic treatment, cooling to room temperature, then weighing again, complementing lost mass with 70% methanol, shaking up, filtering, and taking a subsequent filtrate to obtain a test sample solution;
s3, content determination:
respectively measuring chromatograms of the reference solution and the test solution by high performance liquid chromatography under the same detection conditions; and calculating the content of the component corresponding to the reference solution in the red water dendrobium nobile test solution according to the concentration of the reference solution, the peak area of the reference solution in the chromatogram and the peak area of the component corresponding to the reference solution in the test solution in the chromatogram.
2. The method for measuring the content of the components of the red-water dendrobium nobile lindl according to claim 1, wherein in step S1, a proper amount of each standard substance is precisely weighed and respectively placed in a 5 mL volumetric flask, the volume is determined to the scale by 70% methanol, and the mixed reference substance solution containing liquiritin 0.042 mg/mL, kaempferol-3-glucose rhamnoside 0.132 mg/mL and isoquercitrin 0.040 mg/mL is prepared by ultrasonic oscillation and shaking up.
3. The method for measuring the content of red water dendrobium nobile lindl flower components according to claim 1, wherein in step S2, dried dendrobium nobile lindl flower 0.5 g is accurately weighed into a conical flask, 35 mL of 70% methanol is added, weighing and shaking are performed uniformly, ultrasound is performed for 60 min (45 kHz, 90%), the dendrobium nobile lindl flower is weighed after being cooled to room temperature, lost mass is complemented with 70% methanol, shaking is performed uniformly, and filtration is performed to obtain a subsequent filtrate to obtain a sample solution.
4. The method for measuring the content of the flower components of dendrobium nobile lindl according to claim 1, wherein in step S3, the detection conditions comprise: chromatography column Agilent Eclipse Plus C18(4.6 mm. times.150 mm, 5 μm); the flow rate is 1.0 mL/min; the detection wavelength is 280 nm; the column temperature is 30 ℃; gradient elution was performed with 0.06% acetic acid-water solution as mobile phase a and acetonitrile as mobile phase B.
5. The method for measuring the content of the flower components of dendrobium nobile lindl according to claim 1, wherein in step S3, the changes of the mobile phase a and the mobile phase B during the gradient elution are as follows: according to the volume fraction, the time is 0-4 min, 95% A → 87.5A; 4-14 min, 87.5% A → 83% A; 14-21 min, 83% A → 73% A; 21-25 min, 73% A → 0% A.
6. The method of claim 1, wherein in step S3, the mixed control solution 1, 2, 5, 10, 15, 20 μ L prepared in step S1 is precisely measured and injected into a high performance liquid chromatograph for measurement; and drawing a standard curve by taking the sample amount as a horizontal coordinate (X) and the peak area integral value as a vertical coordinate (Y), and calculating a regression equation, a linear range and a regression coefficient of each mixed reference substance.
7. The method for measuring the content of the flower components of dendrobium nobile lindl according to claim 1, wherein in step S3, the content measurement instrument performs a precision test, the mixed reference solution prepared in step S1 is continuously injected for 6 times under the chromatographic condition, 3 reference peak areas are measured, and the retention time and the RSD value of the peak area are calculated respectively.
8. The method for measuring the content of dendrobium nobile lindl flower components according to claim 1, wherein in step S3, in the content measurement repeatability test, 6 parts of the same dendrobium nobile lindl flower sample is taken to prepare a sample solution, the sample is injected under the same chromatographic condition, and the RSD values of retention time and peak area are calculated respectively.
9. The method of claim 1, wherein in step S3, the content determination stability test is performed by taking the same sample of Dendrobium nobile flower, preparing a test solution according to the method in step S2, injecting samples at 0, 4, 8, 12, 16, 20, and 24 hours respectively under the same chromatographic conditions, measuring the peak areas of 3 reference samples, and calculating the RSD values of retention time and peak areas respectively.
10. The method for measuring the content of the dendrobium nobile lindl flower components according to claim 1, wherein in the step S3, the same sample powder with known content is precisely weighed to be 0.25 g, 9 parts in total is precisely added according to the ratio of 1:0.8, 1:1 and 1:1.2 in the content measurement sample-adding recovery test, the sample solution is prepared according to the method for preparing the sample solution in the step S2, the sample solution is respectively measured for 3 times under chromatographic conditions, the peak area of each component is recorded, and the recovery is calculated.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115508472A (en) * 2022-09-30 2022-12-23 江苏集萃新型药物制剂技术研究所有限公司 Multi-component quantitative detection method for dendrobium huoshanense

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101726547A (en) * 2008-10-15 2010-06-09 中山市中智药业集团有限公司 Dendrobe chromatogram finger print measuring method
US20150017734A1 (en) * 2013-07-12 2015-01-15 Hong Kong Baptist University Quality control marker and its use in herbs authentication
CN111650308A (en) * 2020-07-13 2020-09-11 贵州中医药大学 HPLC fingerprint spectrum construction method of dendrobium nobile flowers

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101726547A (en) * 2008-10-15 2010-06-09 中山市中智药业集团有限公司 Dendrobe chromatogram finger print measuring method
US20150017734A1 (en) * 2013-07-12 2015-01-15 Hong Kong Baptist University Quality control marker and its use in herbs authentication
CN111650308A (en) * 2020-07-13 2020-09-11 贵州中医药大学 HPLC fingerprint spectrum construction method of dendrobium nobile flowers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SHIGANG ZHENG 等: "Quantitative assessment of secondary metabolites and cancer cell inhibiting activity by high performance liquid chromatography fingerprinting in Dendrobium nobile" *
李杰 等: "石斛属植物种质资源鉴定及指纹图谱应用研究进展" *
欧焕娇 等: "金钗石斛中黄酮类和酚类成分HPLC指纹图谱研究" *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115508472A (en) * 2022-09-30 2022-12-23 江苏集萃新型药物制剂技术研究所有限公司 Multi-component quantitative detection method for dendrobium huoshanense

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