CN118310967A - Multimode ultrafast optical microscopic imaging system - Google Patents
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Abstract
The invention provides a multi-mode ultrafast optical microscopic imaging system, which comprises a laser output module, a microscopic imaging module and a light path adjusting component, wherein the laser output module is connected with the microscopic imaging module; the laser output module is used for generating and outputting pump light and detection light with optical path difference; the microscopic imaging module comprises a sample placing platform, an objective lens, a cage-type cube, a wavelength selection module and a detector which are sequentially arranged along a Y axis; the cage-type cube is arranged on the pump light emergent light path, and the sample placing platform and the objective lens are arranged between the pump light emergent light path and the detection light emergent light path; the light path adjusting component is used for adjusting the irradiation modes of the pump light and the detection light to the sample to be detected so as to enable the system to work in different optical imaging modes. The invention integrates three ultra-fast optical imaging modes of reflection, transmission and fluorescence, and can realize the fast switching among the three working modes by carrying out simple light path fine adjustment through the light path adjusting component.
Description
Technical Field
The invention relates to the technical field of optical microscopic imaging, in particular to a multi-mode ultrafast optical microscopic imaging system.
Background
Ultrafast spectroscopy techniques are widely used to study ultrafast kinetic processes of system excited states, which can provide rich spectral features and ultrafast time kinetic information in both time and frequency dimensions. The most widely used ultra-fast spectroscopy technology is the transient absorption (also called pump-detection) spectroscopy technology at present, and the principle is that a substance is pumped (excited) by using femtosecond laser pulses, so that the substance enters an excited state, and the detailed dynamics process of transition of molecules of the substance from the excited state to other low energy levels or ground states is recorded through the interaction of the laser pulses and the substance in a femtosecond time scale. The technology provides a powerful tool for researching material science and is widely applied to researches in aspects of material science, physical chemistry, biology and the like.
In conventional ultrafast spectroscopy techniques, spatially resolved spectral information is missing. In recent years, with the rapid development of the front-end technological fields such as perovskite photovoltaics, two-dimensional materials, quantum devices, high-temperature superconductivity and the like, deep disclosure of migration and evolution transport mechanisms of microscopic carriers in a space dimension is urgently needed, and heterogeneity of physical states of micro-nano materials in space distribution is explored. The ultra-fast optical microscopic imaging technology combines the traditional ultra-fast spectroscopy technology with the optical microscopy technology, is an important tool for researching the space-time evolution of microscopic particles and energy and explaining a microscopic mechanism, can research the motion and evolution of the microscopic particles and the energy in two dimensions of space and time, and meets the requirement of deep research on the microscopic particles such as carriers in the space dimension.
However, conventional ultrafast optical microscopes are typically capable of testing only a single ultrafast spectroscopic signal, such as a transient absorption microscope, confocal fluorescence lifetime imaging microscope, and the like. To perform multidimensional ultrafast optical signal microscopic characterization on a sample, switching among multiple sets of test systems is often required; the test flow is tedious and time-consuming, in-situ test cannot be realized, and high requirements are provided for stability and uniformity of the sample; moreover, the different excitation conditions in different test systems increase the difficulty of data analysis.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a multi-mode ultrafast optical microscopic imaging system, which integrates three ultrafast optical imaging modes of reflection, transmission and fluorescence into a whole and realizes the rapid switching among the three working modes through simple light path fine adjustment.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
A multimode ultrafast optical microscopic imaging system comprises a laser output module, a microscopic imaging module and a light path adjusting component;
the laser output module is used for generating and outputting pump light and detection light with optical path difference; the pump light emergent light path and the probe light emergent light path are parallel to the X axis and are spaced a certain distance from each other;
The microscopic imaging module comprises a sample placing platform, an objective lens, a cage-type cube, a wavelength selection module and a detector which are sequentially arranged along a Y axis; the cage-type cube is arranged on the pump light emergent light path, and the sample placing platform and the objective lens are arranged between the pump light emergent light path and the detection light emergent light path;
the sample placing platform is used for placing and fixing a sample to be tested;
a dichroic mirror or a polarization selection prism is arranged in the cage-type cube and is used for reflecting and turning a light beam projected in the X-axis direction and then projecting the light beam onto a sample to be detected in the Y-axis direction;
the objective lens is used for focusing the light beam projected by the cage cube on the sample to be detected;
After the sample to be detected on the sample placing platform is irradiated by the pump light and/or the detection light, the reflected or transmitted light beam is a feedback light beam, and the feedback light beam sequentially passes through the objective lens, the cage-type cube and the wavelength selection module along the Y axis and then is projected onto the detector;
The wavelength selection module is composed of a bandpass filter and/or a polaroid and is used for filtering pump light in the feedback light beam, and only detection light or fluorescence with specified wavelength is reserved to enter the detector;
the detector is used for carrying out optical imaging on the feedback light beam after the pump light is filtered;
The light path adjusting component is used for adjusting the irradiation modes of the pump light and the detection light to the sample to be detected so as to enable the system to work in different optical imaging modes; the light path adjusting component comprises a light shielding plate, a first lens, a second lens, a beam combining module and a first reflecting mirror;
When the system works in a reflection mode, the beam combination module is arranged on a pump light emergent light path between the laser output module and the cage cube, and the first lens and the first reflector are sequentially arranged on a detection light emergent light path; the first reflecting mirror is arranged opposite to the beam combining module on the Y axis so as to project the detection light to the beam combining module; the beam combination module is used for combining the pump light and the detection light to enable the pump light and the detection light to be overlapped in space, and then the pump light and the detection light are projected to the cage-type cube along the X-axis direction; the cage-type cube reflects the combined pump light and the probe light together, turns the pump light and the probe light to the same side of the sample to be measured along the Y axis and projects the pump light and the probe light to the same side of the sample to be measured;
when the system works in a transmission mode, the first lens and the first reflecting mirror are sequentially arranged on the emergent light path of the detection light; the pump light is projected to one side of the sample to be detected through the cage cube and the objective lens in sequence; the first reflecting mirror is arranged opposite to the sample placing platform on the Y axis so as to project detection light to the opposite side of the sample to be detected;
when the system works in a fluorescence mode, the light shielding plate is arranged in a detection light generation light path of the laser output module so as to block the generation and output of the detection light, and the second lens is arranged on a pump light emergent light path between the laser output module and the cage cube; the pump light is projected to one side of the sample to be measured through the second lens, the cage cube and the objective lens in sequence.
Further, the first lens and the second lens are convex lenses or concave lenses; the first lens is used for enabling the focus of the detection light to deviate from the surface of the sample to be detected, and ensuring that the detection light spots on the surface of the sample to be detected are larger than the pumping light spots so as to realize wide-field imaging; the second lens is used for adjusting the size of the pump light on the surface of the sample to be tested so as to realize wide-field excitation.
Further, the laser output module comprises a laser, a beam splitter, a pump light generation module, an optical delay line and a detection light generation module;
the laser, the beam splitter and the pump light generation module are sequentially arranged along a first straight line; the optical delay line and the detection light generation module are sequentially arranged along a second straight line; the first straight line and the second straight line are parallel to the X axis and are spaced a certain distance from each other;
the laser is used for generating ultrashort pulse laser;
The beam splitter is arranged at the output end of the laser and is used for dividing ultra-short pulse laser emitted by the laser into transmitted light and reflected light with a certain light intensity ratio; the transmission light passes through the beam splitter along a first straight line and then is input to the pump light generation module, the reflection light is reflected by the beam splitter, turned to be input to the optical delay line along a Y axis, and the reflection light is input to the detection light generation module along a second straight line by the optical delay line;
the pump light generation module is used for performing wavelength tuning on the transmitted light so as to generate pump light;
the optical delay line is used for adjusting the optical delay of the reflected light so as to change the optical path difference of the pump light and the detection light reaching the sample to be detected, thereby realizing time resolution;
The detection light generation module is used for wavelength tuning of the reflected light to generate white light as detection light.
Further, the laser output module further comprises a pulse modulation module arranged between the beam splitter and the pump light generation module, wherein the pulse modulation module is a chopper or an electronic shutter and is used for periodically shielding the transmission light input to the pump light generation module by the beam splitter when the system works in a reflection mode or a transmission mode so as to realize the pulse modulation of the pump light.
Further, the light shielding plate is connected to the first mounting platform in a sliding manner, and the first mounting platform extends along the X-axis direction and is intersected with a reflected light path between the beam splitter and the optical delay line; the light shielding plate is used for shielding the reflected light path or removing the reflected light path by sliding on the first mounting platform along the X-axis direction;
the second lens is connected to the second mounting platform in a sliding manner, and the beam combination module is connected to the third mounting platform in a sliding manner; the second mounting platform and the third mounting platform extend along the Y-axis direction and are crossed with a pump light emergent light path between the pump light generating module and the cage cube; the second lens and the beam combining module are respectively added into or removed from the pump light emergent light path by sliding on the second mounting platform and the third mounting platform along the Y-axis direction;
The first reflecting mirror is connected to a fourth mounting platform in a sliding manner, and the fourth mounting platform is arranged on the detection light emergent light path and extends along the X-axis direction; the first reflecting mirror slides on the fourth mounting platform along the X-axis direction to reflect and turn the detection light and then project the detection light to the beam combination module or the sample placing platform along the Y-axis.
Further, the optical delay line comprises an electric translation stage and a hollow retroreflector arranged on the electric translation stage; the motorized translation stage is controlled by software programming.
Further, the laser is a femtosecond pulse laser or a picosecond pulse laser.
Further, the pump light generating module is an optical parametric amplifier, a nonlinear crystal frequency multiplier or a hollow optical fiber spectrum stretcher;
The detection light generating module is an optical parametric amplifier, a super-continuous white light generating nonlinear crystal or a hollow optical fiber spectrum stretcher.
Further, the wavelength selection module is a long-pass filter, a band-pass filter, a linear polarizer or a monochromator.
Further, the detector is a high frame rate area camera or a time resolved single photon area camera.
The multimode ultra-fast optical microscopic imaging system integrates three ultra-fast optical imaging modes of reflection, transmission and fluorescence, and can realize the fast switching among the three working modes under the condition of not moving a sample by carrying out simple light path fine adjustment through the light path adjusting component. Through the structure, the invention simplifies the flow of carrying out multidimensional ultrafast optical signal microscopic characterization test on the sample to be tested, realizes in-situ test on the sample to be tested, ensures the stability and uniformity of the sample to be tested under different test modes, and reduces the difficulty of data analysis.
The invention adopts modularized design, thus being applicable to various experimental scenes; by introducing the light path adjusting component and the microscopic imaging module, the light path adjusting component and the microscopic imaging module can be rapidly switched among three wide-field microscopic imaging modes of reflection, transmission and fluorescence on the basis of not changing the original ultra-fast spectrum test light path, and a sample to be tested does not need to be moved in the mode switching process. The invention is suitable for the traditional low-repetition-frequency titanium sapphire laser system and the high-repetition-frequency ultrafast optical fiber laser system, and provides a reliable solution for in-situ multi-mode ultrafast optical wide-field microscopic imaging.
Drawings
Fig. 1 is a schematic structural diagram of a multi-mode ultrafast optical microscopic imaging system according to an embodiment of the present invention when the system is operated in a reflection mode.
Fig. 2 is a schematic structural diagram of a multi-mode ultrafast optical microscopic imaging system according to an embodiment of the present invention when the system is operated in a transmission mode.
Fig. 3 is a schematic structural diagram of a multi-mode ultrafast optical microscopic imaging system according to an embodiment of the present invention when the system is operated in a fluorescence mode.
Detailed Description
The technical scheme of the invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
As shown in fig. 1 to 3, the multi-mode ultrafast optical microscopic imaging system provided by the embodiment of the invention comprises a laser output module, a microscopic imaging module and an optical path adjusting component;
the laser output module is used for generating and outputting pump light and detection light with optical path difference; the pump light emergent light path and the probe light emergent light path are parallel to the X axis and are spaced a certain distance from each other;
the microscopic imaging module comprises a sample placing platform 15, an objective lens 8, a cage-type cube 9, a wavelength selection module 10 and a detector 11 which are sequentially arranged along a Y axis; the cage-type cube 9 is arranged on the pump light emergent light path, and the sample placement platform 15 and the objective lens 8 are arranged between the pump light emergent light path and the probe light emergent light path;
the sample placing platform 15 is used for placing and fixing a sample to be tested;
An optical element with wavelength or polarization selection, such as a dichroic mirror or a polarization selection prism, is arranged in the cage-type cube 9, and is used for reflecting and turning a light beam projected in the X-axis direction and then projecting the light beam onto a sample to be detected in the Y-axis direction;
the objective lens 8 is used for focusing the light beam projected by the cage cube 9 on the sample to be detected;
After the sample to be detected on the sample placing platform 15 is irradiated by the pump light and/or the detection light, the reflected or transmitted light beam is a feedback light beam, and the feedback light beam sequentially passes through the objective lens 8, the cage cube 9 and the wavelength selection module 10 along the Y axis and then is projected onto the detector 11;
the wavelength selection module 10 is composed of a bandpass filter and/or a polaroid, and is used for filtering pump light in the feedback light beam, and only the detection light or fluorescence with a specified wavelength is reserved to enter the detector 11;
the detector 11 is used for optical imaging of the feedback beam after filtering the pump light;
the light path adjusting component is used for adjusting the irradiation modes of the pump light and the detection light to the sample to be detected so as to enable the system to work in different optical imaging modes; the light path adjusting component comprises a light shielding plate, a first lens 7, a second lens 13, a beam combining module 12 and a first reflecting mirror 14;
When the system works in a reflection mode, the beam combination module 12 is arranged on a pump light emergent light path between the laser output module and the cage cube 9, and the first lens 7 and the first reflecting mirror 14 are sequentially arranged on a detection light emergent light path; the first reflecting mirror 14 is disposed opposite to the beam combining module 12 on the Y axis to project the probe light to the beam combining module 12; the beam combining module 12 is composed of a reflecting mirror group and optical elements with beam combining function, such as a dichroic mirror, a prism, a grating and the like, and is used for combining the pump light and the detection light to realize the superposition in space, and then the pump light and the detection light are projected to the cage-type cube 9 along the X-axis direction; the cage cube 9 reflects the pump light and the detection light after beam combination together, turns the pump light and the detection light and projects the pump light and the detection light to the same side of the sample to be detected along the Y axis;
When the system works in a transmission mode, the first lens 7 and the first reflecting mirror 14 are sequentially arranged on the emergent light path of the detection light; the pump light is projected to one side of the sample to be detected through the cage-type cube 9 and the objective lens 8 in sequence; the first reflecting mirror 14 is arranged opposite to the sample placing platform 15 on the Y axis so as to project the detection light to the opposite side of the sample to be detected;
When the system works in a fluorescence mode, the light shielding plate is arranged in a detection light generation light path of the laser output module so as to block the generation and output of the detection light, and the second lens 13 is arranged on a pump light emergent light path between the laser output module and the cage cube 9; the pump light is projected to one side of the sample to be measured through the second lens 13, the cage cube 9 and the objective lens 8 in sequence.
Wherein the first lens 7 and the second lens 13 are convex lenses or concave lenses with long focal length; the first lens 7 is used for deviating the focus of the detection light from the surface of the sample to be detected, so that the detection light spot on the surface of the sample to be detected is far greater than the pumping light spot, and wide-field imaging is realized; the second lens 13 is used for adjusting the size of the pump light on the surface of the sample to be tested so as to realize wide-field excitation.
Specifically, the laser output module comprises a laser 1, a beam splitter 2, a pump light generation module 4, an optical delay line 5 and a detection light generation module 6;
The laser 1, the beam splitter 2 and the pump light generation module 4 are sequentially arranged along a first straight line; the optical delay line 5 and the detection light generation module 6 are sequentially arranged along a second straight line; the first straight line and the second straight line are parallel to the X axis and are spaced a certain distance from each other;
The laser 1 is used for generating ultra-short pulse laser;
The beam splitter 2 is arranged at the output end of the laser 1 and is used for splitting ultra-short pulse laser emitted by the laser 1 into transmitted light and reflected light with a certain light intensity ratio; the transmitted light passes through the beam splitter 2 along a first straight line and then is input to the pump light generation module 4, and the reflected light is reflected and turned by the beam splitter 2 and then is input to the optical delay line 5 along a Y axis and is input to the probe light generation module 6 along a second straight line by the optical delay line 5;
the pump light generation module 4 is used for wavelength tuning of the transmitted light so as to generate pump light;
The optical delay line 5 is used for adjusting the optical delay of the reflected light so as to change the optical path difference of the pump light and the detection light reaching the sample to be detected, thereby realizing time resolution;
The detection light generation module 6 is configured to wavelength tune the reflected light to generate white light as detection light.
Further, the laser output module further includes a pulse modulation module 3 disposed between the beam splitter 2 and the pump light generating module 4, where the pulse modulation module 3 is a chopper or an electronic shutter, and is configured to periodically block the transmitted light input to the pump light generating module 4 by the beam splitter 2 when the system works in a reflection mode or a transmission mode, so as to implement pulse modulation of the pump light.
As an improvement, in order to facilitate rapid adjustment of the optical path adjusting component, the light shielding plate is slidably connected to a first mounting platform, and the first mounting platform extends along the X-axis direction and intersects with the reflected light path between the beam splitter 2 and the optical delay line 5; the light shielding plate is used for shielding the reflected light path or removing the reflected light path by sliding on the first mounting platform along the X-axis direction;
The second lens 13 is slidably connected to the second mounting platform, and the beam combining module 12 is slidably connected to the third mounting platform; the second mounting platform and the third mounting platform extend along the Y-axis direction and are crossed with a pump light emergent light path between the pump light generating module 4 and the cage cube 9; the second lens 13 and the beam combining module 12 are respectively added to or removed from the pump light emergent light path by sliding along the Y-axis direction on the second mounting platform and the third mounting platform;
The first reflecting mirror 14 is slidably connected to a fourth mounting platform, and the fourth mounting platform is disposed on the detection light emitting optical path and extends along the X-axis direction; the first reflecting mirror 14 slides on the fourth mounting platform along the X-axis direction to reflect and redirect the detection light to be projected to the beam combining module 12 or the sample placing platform 15 along the Y-axis.
In this embodiment, the optical delay line 5 includes an electromotive translation stage and a hollow retro-reflective mirror disposed on the electromotive translation stage; the motorized translation stage is controlled by software programming.
The laser 1 is a femtosecond pulse laser or a picosecond pulse laser.
The pump light generating module 4 is a device with wavelength conversion and regulation functions, such as an optical parametric amplifier, a nonlinear crystal frequency multiplier or a hollow optical fiber spectrum stretcher;
The detection light generating module 6 is an optical parametric amplifier, a super-continuous white light generating nonlinear crystal or a hollow optical fiber spectrum stretcher and other devices with wavelength conversion and regulation functions.
The wavelength selection module 10 is a component or device having a spectrum or polarization filtering function, such as a long-pass filter, a band-pass filter, a linear polarizer, or a monochromator.
The detector 11 is a high frame rate area camera or a time resolved single photon area camera.
The multimode ultra-fast optical microscopic imaging system provided by the invention has the greatest advantages that three ultra-fast optical imaging modes including reflection, transmission and fluorescence are integrated, and the rapid switching among the three working modes can be realized under the condition that a sample does not need to be moved by carrying out simple light path fine adjustment through the light path adjusting component.
Specifically, as shown in fig. 1, when the system is operated in the reflection mode, the light shielding plate is removed from the probe light generation optical path, the second lens 13 is removed from the pump light exit optical path, the beam combining module 12 is added to the pump light exit optical path, and the first reflecting mirror 14 is moved to be opposite to the beam combining module 12 in the Y-axis direction, so that the setting can be completed. During operation, the probe light enters the beam combining module 12 through the reflecting mirror to combine with the pump light, and the pump light and the probe light are overlapped in space. The pump light and the detection light emitted from the beam combination module 12 are reflected and turned by a dichroic mirror or a polarization selection prism in the cage type cube 9, focused by the objective lens 8 and incident on the surface of the sample to be detected. The feedback light beam reflected by the sample to be detected (namely, the reflected light beam of the sample to be detected to the pump light and the detection light) is collected by the objective lens 8 and is incident to the cage cube 9, the pump light reflected by the surface of the sample to be detected is filtered by the wavelength selection module 10 after the feedback light beam penetrates through the cage cube 9, only the detection light with a specified wavelength is reserved to enter the detector 11, and the transient reflection signal is calculated in real time through computer programming, so that the multi-mode high-sensitivity time-resolved ultrafast optical reflection signal collection is realized, and the wide-field microscopic imaging is realized.
Referring to fig. 2, when the system is operated in the transmission mode, the light shielding plate is removed from the probe light generating optical path, the second lens 13 and the beam combining module 12 are removed from the pump light emitting optical path, and the first reflecting mirror 14 is moved to face the sample to be measured in the Y-axis direction, so that the setting can be completed. During operation, the pump light sequentially passes through the cage cube 9 and the objective lens 8 and then is projected on one side of the Y-axis direction of the sample to be detected, and the detection light sequentially passes through the first lens 7 and the first reflecting mirror 14 and then is projected on the other side of the Y-axis direction of the sample to be detected. The detection light transmitted through the sample to be detected further passes through the objective lens 8, the cage cube 9 and the wavelength selection module 10 and then reaches the detector 11, and transient transmission or absorption signals are calculated in real time through computer programming, so that multi-mode high-sensitivity time-resolved ultra-fast optical transmission or absorption signal acquisition is realized, and wide-field microscopic imaging is realized.
As shown in fig. 3, when the system is operated in the fluorescence mode, a light shielding plate is added to the probe light generation optical path to block the generation and output of the probe light, the beam combining module 12 is removed from the pump light exit optical path, and the second lens 13 is added to the pump light exit optical path, so that the setting can be completed. During operation, the light shielding plate blocks the reflected light beam of the beam splitter 2, so as to inhibit the generation of detection light, the pulse modulation module 3 is turned off or removed to continuously output pumping light, and the pumping light sequentially passes through the second lens 13, the cage cube 9 and the objective lens 8 and then is projected onto the surface of the sample to be detected. The second lens 13 is used for adjusting the size of a light spot formed by the pump light on the surface of the sample to be tested, so as to realize wide-field excitation. The fluorescence emitted by the surface of the sample to be measured after being irradiated by the pumping light sequentially passes through the objective lens 8, the cage cube 9 and the wavelength selection module 10 and then reaches the detector 11, so that the fluorescence wide-field microscopic imaging function is realized. Further, if time-resolved fluorescence wide-field imaging needs to be achieved, the detector 11 is replaced by an area-array camera with a time-resolved single photon counting function, so that multi-mode high-sensitivity time-resolved ultrafast optical fluorescence signal acquisition and wide-field microscopic imaging are achieved.
The multimode ultra-fast optical microscopic imaging system integrates three ultra-fast optical imaging modes of reflection, transmission and fluorescence, and can realize the fast switching among the three working modes under the condition of not moving a sample by carrying out simple light path fine adjustment through the light path adjusting component. Through the structure, the invention simplifies the flow of carrying out multidimensional ultrafast optical signal microscopic characterization test on the sample to be tested, realizes in-situ test on the sample to be tested, ensures the stability and uniformity of the sample to be tested under different test modes, and reduces the difficulty of data analysis.
The invention adopts modularized design, thus being applicable to various experimental scenes; by introducing the light path adjusting component and the microscopic imaging module, the light path adjusting component and the microscopic imaging module can be rapidly switched among three wide-field microscopic imaging modes of reflection, transmission and fluorescence on the basis of not changing the original ultra-fast spectrum test light path, and a sample to be tested does not need to be moved in the mode switching process. The invention is suitable for the traditional low-repetition-frequency titanium sapphire laser system and the high-repetition-frequency ultrafast optical fiber laser system, and provides a reliable solution for in-situ multi-mode ultrafast optical wide-field microscopic imaging.
Furthermore, in the reflection mode and the transmission mode, the invention adopts the high-frame-rate area array camera as the detector for ultra-fast optical imaging, the highest frame rate is more than 1000 frames per second, and for the traditional 1kHz heavy-frequency laser system, single exposure plane imaging can be realized, and the imaging time is greatly shortened; the high-speed ultrafast optical signal plane imaging is realized, and the imaging efficiency is improved; by shortening the data acquisition time, the requirements on the stability of the laser light source and the sample are reduced. For the weak signal and time-resolved fluorescence mode, the time-resolved single-photon area array camera is used as an imaging detector, so that the sensitivity is improved to the single photon level, and the signal-to-noise ratio of the weak signal is greatly improved.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (10)
1. The multimode ultrafast optical microscopic imaging system is characterized by comprising a laser output module, a microscopic imaging module and an optical path adjusting component;
the laser output module is used for generating and outputting pump light and detection light with optical path difference; the pump light emergent light path and the probe light emergent light path are parallel to the X axis and are spaced a certain distance from each other;
The microscopic imaging module comprises a sample placing platform, an objective lens, a cage-type cube, a wavelength selection module and a detector which are sequentially arranged along a Y axis; the cage-type cube is arranged on the pump light emergent light path, and the sample placing platform and the objective lens are arranged between the pump light emergent light path and the detection light emergent light path;
the sample placing platform is used for placing and fixing a sample to be tested;
a dichroic mirror or a polarization selection prism is arranged in the cage-type cube and is used for reflecting and turning a light beam projected in the X-axis direction and then projecting the light beam onto a sample to be detected in the Y-axis direction;
the objective lens is used for focusing the light beam projected by the cage cube on the sample to be detected;
After the sample to be detected on the sample placing platform is irradiated by the pump light and/or the detection light, the reflected or transmitted light beam is a feedback light beam, and the feedback light beam sequentially passes through the objective lens, the cage-type cube and the wavelength selection module along the Y axis and then is projected onto the detector;
The wavelength selection module is composed of a bandpass filter and/or a polaroid and is used for filtering pump light in the feedback light beam, and only detection light or fluorescence with specified wavelength is reserved to enter the detector;
the detector is used for carrying out optical imaging on the feedback light beam after the pump light is filtered;
The light path adjusting component is used for adjusting the irradiation modes of the pump light and the detection light to the sample to be detected so as to enable the system to work in different optical imaging modes; the light path adjusting component comprises a light shielding plate, a first lens, a second lens, a beam combining module and a first reflecting mirror;
When the system works in a reflection mode, the beam combination module is arranged on a pump light emergent light path between the laser output module and the cage cube, and the first lens and the first reflector are sequentially arranged on a detection light emergent light path; the first reflecting mirror is arranged opposite to the beam combining module on the Y axis so as to project the detection light to the beam combining module; the beam combination module is used for combining the pump light and the detection light to enable the pump light and the detection light to be overlapped in space, and then the pump light and the detection light are projected to the cage-type cube along the X-axis direction; the cage-type cube reflects the combined pump light and the probe light together, turns the pump light and the probe light to the same side of the sample to be measured along the Y axis and projects the pump light and the probe light to the same side of the sample to be measured;
when the system works in a transmission mode, the first lens and the first reflecting mirror are sequentially arranged on the emergent light path of the detection light; the pump light is projected to one side of the sample to be detected through the cage cube and the objective lens in sequence; the first reflecting mirror is arranged opposite to the sample placing platform on the Y axis so as to project detection light to the opposite side of the sample to be detected;
when the system works in a fluorescence mode, the light shielding plate is arranged in a detection light generation light path of the laser output module so as to block the generation and output of the detection light, and the second lens is arranged on a pump light emergent light path between the laser output module and the cage cube; the pump light is projected to one side of the sample to be measured through the second lens, the cage cube and the objective lens in sequence.
2. The multi-modality ultrafast optical microimaging system of claim 1, wherein the first and second lenses are convex or concave lenses; the first lens is used for enabling the focus of the detection light to deviate from the surface of the sample to be detected, and ensuring that the detection light spots on the surface of the sample to be detected are larger than the pumping light spots so as to realize wide-field imaging; the second lens is used for adjusting the size of the pump light on the surface of the sample to be tested so as to realize wide-field excitation.
3. The multi-mode ultrafast optical microscopic imaging system of claim 1, wherein the laser output module comprises a laser, a beam splitter, a pump light generation module, an optical delay line, and a probe light generation module;
the laser, the beam splitter and the pump light generation module are sequentially arranged along a first straight line; the optical delay line and the detection light generation module are sequentially arranged along a second straight line; the first straight line and the second straight line are parallel to the X axis and are spaced a certain distance from each other;
the laser is used for generating ultrashort pulse laser;
The beam splitter is arranged at the output end of the laser and is used for dividing ultra-short pulse laser emitted by the laser into transmitted light and reflected light with a certain light intensity ratio; the transmission light passes through the beam splitter along a first straight line and then is input to the pump light generation module, the reflection light is reflected by the beam splitter, turned to be input to the optical delay line along a Y axis, and the reflection light is input to the detection light generation module along a second straight line by the optical delay line;
the pump light generation module is used for performing wavelength tuning on the transmitted light so as to generate pump light;
the optical delay line is used for adjusting the optical delay of the reflected light so as to change the optical path difference of the pump light and the detection light reaching the sample to be detected, thereby realizing time resolution;
The detection light generation module is used for wavelength tuning of the reflected light to generate white light as detection light.
4. The multi-mode ultrafast optical microscopic imaging system of claim 3, wherein the laser output module further comprises a pulse modulation module arranged between the beam splitter and the pump light generation module, the pulse modulation module is a chopper or an electronic shutter, and is used for periodically shielding the transmitted light input to the pump light generation module by the beam splitter when the system works in a reflection mode or a transmission mode so as to realize pulse modulation of the pump light.
5. The multi-mode ultrafast optical microscopic imaging system according to claim 3, wherein the light shielding plate is slidably connected to a first mounting platform, and the first mounting platform extends along the X-axis direction and intersects with a reflected light path between the beam splitter and the optical delay line; the light shielding plate is used for shielding the reflected light path or removing the reflected light path by sliding on the first mounting platform along the X-axis direction;
the second lens is connected to the second mounting platform in a sliding manner, and the beam combination module is connected to the third mounting platform in a sliding manner; the second mounting platform and the third mounting platform extend along the Y-axis direction and are crossed with a pump light emergent light path between the pump light generating module and the cage cube; the second lens and the beam combining module are respectively added into or removed from the pump light emergent light path by sliding on the second mounting platform and the third mounting platform along the Y-axis direction;
The first reflecting mirror is connected to a fourth mounting platform in a sliding manner, and the fourth mounting platform is arranged on the detection light emergent light path and extends along the X-axis direction; the first reflecting mirror slides on the fourth mounting platform along the X-axis direction to reflect and turn the detection light and then project the detection light to the beam combination module or the sample placing platform along the Y-axis.
6. The multi-modality ultrafast optical microscopic imaging system of claim 3, wherein the optical delay line includes an motorized translation stage and a hollow retro-reflective mirror disposed on the motorized translation stage; the motorized translation stage is controlled by software programming.
7. A multi-mode ultrafast optical microimaging system as in claim 3, wherein the laser is a femtosecond pulse laser or a picosecond pulse laser.
8. The multi-mode ultrafast optical microscopic imaging system of claim 3, wherein the pump light generating module is an optical parametric amplifier, a nonlinear crystal frequency multiplier, or a hollow fiber spectral stretcher;
The detection light generating module is an optical parametric amplifier, a super-continuous white light generating nonlinear crystal or a hollow optical fiber spectrum stretcher.
9. The multi-mode ultrafast optical microscopic imaging system of claim 3, wherein the wavelength selection module is a long pass filter, a band pass filter, a linear polarizer, or a monochromator.
10. The multi-modality ultrafast optical microscopic imaging system of claim 3, wherein the detector is a high frame rate area array camera or a time resolved single photon area array camera.
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| Publication Number | Publication Date |
|---|---|
| CN118310967A true CN118310967A (en) | 2024-07-09 |
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