CN118191070A - Biological index concentration transient detection system based on human body fluid - Google Patents
Biological index concentration transient detection system based on human body fluid Download PDFInfo
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Abstract
The invention provides a biological index concentration transient detection system based on human body fluid, and relates to the technical field of detection systems. The biological index concentration transient detection system based on the human body fluid comprises a high-sensitivity electrochemical detector, an enzyme-free kit and a sample injection pen; the enzyme-free kit comprises an enzyme-free three-electrode solid sensor; the high-sensitivity electrochemical detector is provided with a self-priming base, the self-priming base is provided with a three-electrode elastic contact, and the three-electrode elastic contact is in signal connection with the enzyme-free three-electrode solid sensor; the sample injection pen is used for controlling the sample injection pen to quantitatively absorb or inject the human body fluid sample to the surface of the enzyme-free three-electrode solid sensor at a constant speed, and the high-sensitivity electrochemical detector is used for collecting the instantaneous electric signals so as to detect the concentration of the biological index. The detection system provided by the invention solves the problems that the household detector in the prior art needs alcohol disinfection for blood taking, needs microneedle wound, is easy to cause wound infection and the like.
Description
Technical Field
The invention relates to the technical field of detection systems, in particular to a biological index concentration transient detection system based on human body fluid.
Background
Diabetes is a hidden disease and blood glucose monitoring is a guarantee of blood glucose control for patients. The blood glucose detection method popular in the market at present is to take blood by using a blood glucose meter through fingertips and measure by using test paper, wherein serious diabetics need to take blood by the fingertips for 6 times or more a day; frequent collection of blood samples may result in pain, wound infection, or other complications; the readings of the test strip may be affected by user skill, environmental conditions, and the quality of the test strip, resulting in low accuracy of blood glucose measurements, thereby affecting patient treatment decisions and management.
Uric acid is the final product of purine breakdown in nucleic acids in the body, mostly excreted by the kidneys. The diseases such as gout and the like can be caused by elevated uric acid content in blood due to diet, life style, heredity, medicine and diseases. In the uric acid detection method popular in the market at present, a detector is used for collecting blood through fingertips, and test paper is used for measuring the blood; frequent blood sample collection as described above can lead to pain, wound infection, or other complications; the readings of the test strip may be affected by user skill, environmental conditions, and the quality of the test strip, resulting in low accuracy of blood glucose measurements, thereby affecting patient treatment decisions and management.
Disclosure of Invention
The invention aims to provide a biological index concentration transient detection system based on human body fluid, which aims to solve the technical problems of inconvenient use, low accuracy, poor repeatability and need of minimally invasive in a chemical detection method household detector in the prior similar product technology.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
the biological index concentration transient detection system based on human body fluid provided by the invention comprises a high-sensitivity electrochemical detector, an enzyme-free kit and a sample injection pen;
The enzyme-free kit comprises a bottle body and a bottle bottom, wherein the bottle bottom is arranged at the bottom of the bottle body, an enzyme-free three-electrode solid sensor is arranged on the surface, close to the bottle body, of the bottle bottom, and a three-electrode conductive contact is arranged on the other surface of the bottle bottom;
The high-sensitivity electrochemical detector is provided with a self-priming base, the self-priming base is provided with a three-electrode elastic contact, the self-priming base is in limit fit with the enzyme-free kit, and the three-electrode elastic contact is in signal connection with the enzyme-free three-electrode solid sensor;
The sample injection pen comprises a shell, a control mechanism and a constant-speed and quantitative plunger mechanism, wherein the control mechanism is arranged on the shell and is used for controlling the sample injection pen to quantitatively absorb or inject a human body fluid sample to the surface of the enzyme-free three-electrode solid sensor at a constant speed, and the high-sensitivity electrochemical detector is used for collecting instant electric signals so as to detect the concentration of biological indexes; the fixed-speed quantitative plunger mechanism is arranged at the tail end of the sample injection pen.
Still further, the enzyme-free three-electrode solid state sensor comprises a counter electrode, a working electrode, and a reference electrode;
The counter electrode, the working electrode and the reference electrode are arranged at intervals.
Further, the working electrode material is conductive mixed carbon paste; the counter electrode material is conductive carbon paste; the reference electrode material is conductive silver paste.
Still further, the bottle bottom is installed the first magnetic part, from inhaling formula base and install the second magnetic part, first magnetic part with second magnetic part magnetism is opposite.
Still further, the bottle bottom is equipped with first axial location structure, from inhaling formula base and be equipped with second rotation location structure, first axial location structure with second rotation location structure cooperatees.
Still further, the control mechanism comprises a push switch rod, a liquid injection spring, a piston rod and a piston sleeve;
The pressing switch rod is arranged at the top of the shell, one end of the pressing switch rod stretches into the shell, and the piston sleeve is arranged in the shell and is arranged at intervals with the pressing switch rod;
the bottom of push switch pole is equipped with the spout, penetrate the liquid spring and be located in the spout, the one end of piston rod with penetrate liquid spring connection and with spout sliding fit, the piston sleeve cover is located the piston rod, and install the end constant speed ration plunger mechanism.
Further, the control mechanism further comprises a return spring and an outer sleeve, wherein the outer sleeve is positioned in the shell and sleeved on the piston sleeve;
the end, extending into the sliding groove, of the piston rod is provided with a bulge, the outer sleeve is provided with a 7-shaped limit groove, and the bulge is inserted into the limit groove;
One end of the return spring is abutted with the limiting protrusion of the push switch rod, and the other end of the return spring is abutted with the outer sleeve.
Still further, the bottle includes body and bottle lid, the one end of body with the bottle lid is connected, the other end with the bottle bottom is connected.
Further, an installation protrusion is arranged on the inner wall of the bottle body, and an installation groove is arranged on the lower surface of the installation protrusion;
The bottle bottom is adhered to the lower surface of the mounting boss, and the first magnetic piece penetrates through the bottle bottom and is inserted into the mounting groove.
Still further, the body is provided with a third axial positioning structure, and the first axial positioning structure is matched with the third axial positioning structure.
In summary, the technical effects achieved by the invention are analyzed as follows:
The biological index concentration transient detection system based on human body fluid provided by the invention comprises a high-sensitivity electrochemical detector, an enzyme-free kit and a sample injection pen; the enzyme-free kit comprises a bottle body and a bottle bottom, wherein the bottle bottom is arranged at the bottom of the bottle body, an enzyme-free three-electrode solid sensor is arranged on the surface, close to the bottle body, of the bottle bottom, and a three-electrode conductive contact is arranged on the other surface of the bottle bottom; the high-sensitivity electrochemical detector is provided with a self-priming base, the self-priming base is provided with a three-electrode elastic contact, the self-priming base is in limit fit with the enzyme-free kit, and the three-electrode elastic contact is in signal connection with the enzyme-free three-electrode solid sensor; the sample injection pen comprises a shell, a control mechanism and a constant-speed and quantitative plunger mechanism, wherein the control mechanism is arranged on the shell and is used for controlling the sample injection pen to quantitatively absorb or inject a human body fluid sample to the surface of the enzyme-free three-electrode solid sensor at a constant speed, and the high-sensitivity electrochemical detector is used for collecting instantaneous electric signals so as to detect the concentration of biological indexes; the fixed-speed quantitative plunger mechanism is arranged at the tail end of the sample injection pen.
The transient detection is that the spray pen is used to spray the body fluid sample to be detected onto the sensor surface fast in fixed speed, fixed amount and fast, and to collect instantaneous electric signal to obtain corresponding biological index concentration.
The principle of the detection system is as follows: under a certain electrode potential, when liquid flow containing an object to be detected (such as blood sugar, uric acid or vitamins and the like) is sprayed onto the surface of the electrode at a constant speed and in a quantitative manner, the object to be detected can instantaneously generate oxidation-reduction reaction on the surface of the electrode to generate an electric signal, and when the environmental conditions are consistent (the liquid flow spraying speed, the spraying dosage, the spraying distance and the concentration of the solution in the reagent bottle are kept unchanged), the electric signal value and the concentration of the object to be detected are in a linear relationship, so that quantitative analysis can be performed.
The following is exemplified by the use of saliva and the detection of blood glucose and uric acid: and automatically taking a certain amount of saliva by using an injection pen, quantitatively injecting the saliva into a quantitatively self-made sample diluent at a constant speed, and simultaneously applying a certain potential to the electrode to enable the blood sugar/uric acid in the saliva to instantaneously perform oxidation-reduction reaction on the enzyme-free three-electrode solid sensor to generate an electric signal. Under the condition that the liquid flow injection speed, the injection dosage, the injection distance and the solution concentration in the reagent bottle are kept unchanged, the detection system makes a standard curve by comparing the electrical signal values of blood sugar and uric acid standard samples with different concentrations, and obtains the contents of blood sugar and uric acid in the sample to be detected according to the electrical signal values generated by the sample to be detected. The detection system can be used for detecting the concentration of blood sugar and uric acid independently, or detecting the concentration of blood sugar and uric acid independently or simultaneously.
The enzyme-free kit in the detection system is a disposable, portable and pre-packaged kit, and the enzyme-free three-electrode solid sensor is utilized for detecting the concentration of biological indexes, so that the detection system is easy to use, simple to operate and capable of avoiding cross contamination; the method has the advantages of avoiding complex enzyme reaction steps in the traditional method and overcoming the defects of the microneedle wound detection method while realizing high sensitivity and convenience.
The sample injection pen in the detection system has a simple and small structure, can sample trace by one hand, and can rapidly, quantitatively and uniformly inject and sample at a fixed speed, so as to realize transient detection; compared with the existing static detection, the transient detection has high sensitivity and good reproducibility.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of a biological index concentration transient detection system based on human body fluid according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a high-sensitivity electrochemical detector in a detection system according to an embodiment of the present invention;
FIG. 3 is an enlarged view of a portion of FIG. 2 at A;
FIG. 4 is a schematic structural diagram of an enzyme-free kit in a detection system according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of the internal structure of an enzyme-free kit in a detection system according to an embodiment of the present invention;
FIG. 6 is a schematic diagram showing a structure of a bottle bottom in the detecting system according to the embodiment of the present invention;
FIG. 7 is a schematic diagram II of the structure of the bottle bottom in the detection system according to the embodiment of the invention;
fig. 8 is a schematic structural diagram of a bottle body in the detection system according to the embodiment of the present invention;
Fig. 9 is a schematic structural diagram of a bottle cap in the detection system according to the embodiment of the present invention;
FIG. 10 is a schematic diagram of a sample injection pen in a detection system according to an embodiment of the present invention;
FIG. 11 is a schematic diagram showing a sample injection pen in a detection system according to an embodiment of the present invention;
fig. 12 is a schematic diagram of a sample injection pen in a detection system according to an embodiment of the present invention.
Icon:
100-high sensitivity electrochemical detector; 110-self-priming base; 111-three-electrode elastic contacts; 112-a second magnetic member; 113-a second positioning plane; 120-a display unit; 130-a switch; 140-switching keys; 150-charging port;
200-enzyme-free kit; 210-bottle bottom; 211-enzyme-free three-electrode solid sensor; 212-a counter electrode; 213-working electrode; 214-a reference electrode; 215-three electrode conductive contacts; 216—a first magnetic member; 217-a first positioning plane; 220-bottle body; 221-mounting projections; 222-mounting groove; 223-a third positioning plane; 230-bottle cap; 231-an operation section; 232-an anti-slip structure; 233-a connection; 240-centralizer;
300-sample injection pen; 310-a housing; 320-a constant-speed and quantitative plunger mechanism; 330-pressing the switch lever; 331-a chute; 332-limit protrusions; 340-a fluid ejection spring; 350-a piston rod; 351-bump; 360-piston sleeve; 370-return spring; 380-an outer sleeve; 381-limit groove.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal," "vertical," "overhang," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Some embodiments of the present invention are described in detail below with reference to the accompanying drawings. The following embodiments and features of the embodiments may be combined with each other without conflict.
The biological index concentration transient detection system based on the human body fluid provided by the embodiment of the invention comprises a high-sensitivity electrochemical detector 100, an enzyme-free kit 200 and a sample injection pen 300; the enzyme-free kit 200 comprises a bottle body and a bottle bottom 210, wherein the bottle bottom 210 is arranged at the bottom of the bottle body, an enzyme-free three-electrode solid sensor 211 is arranged on the surface close to the bottle body, and a three-electrode conductive contact 215 is arranged on the other surface; the high-sensitivity electrochemical detector 100 is provided with a self-priming base 110, the self-priming base 110 is provided with a three-electrode elastic contact 111, the self-priming base 110 is in limit fit with the enzyme-free kit 200, and the three-electrode elastic contact 111 is in signal connection with an enzyme-free three-electrode solid sensor 211; the sample injection pen 300 comprises a shell 310, a control mechanism and a constant-speed and quantitative plunger mechanism 320, wherein the control mechanism is arranged on the shell 310 and is used for controlling the sample injection pen 300 to quantitatively absorb or inject saliva samples to the surface of the enzyme-free three-electrode solid sensor 211 at a constant speed, and the high-sensitivity electrochemical detector 100 collects instantaneous electric signals so as to detect the concentration of biological indexes; a constant-speed and quantitative plunger mechanism 320 is mounted at the tip of the sample injection pen 300.
Specifically, referring to fig. 1 to 3, the high-sensitivity electrochemical detector 100 includes a housing, an electrical signal processing unit, a display unit 120, a switch 130, a switching key 140 and a charging port 150, wherein the electrical signal processing unit is located in the housing and is in signal connection with the display unit 120, the switch 130, the switching key 140 and the three-electrode elastic contact 111 to send a start signal or receive information. The electrical signal processing unit comprises a microprocessor chip, the microprocessor chip can be provided with a memory unit or is matched with an external memory unit for implementation, the electrical signal processing unit stores a saliva blood sugar/uric acid reference table in advance, the saliva blood sugar/uric acid reference table records the comparison relation between a plurality of blood sugar/uric acid values and electrochemical parameters obtained by the electrical signal processing unit, the electrical signal processing unit can provide the blood sugar/uric acid values based on the saliva blood sugar/uric acid reference table when receiving the electrochemical parameters, and the electrical signal processing unit can correct the saliva blood sugar/uric acid reference table based on the saliva blood sugar/uric acid values and the electrochemical parameters when receiving the correction blood sugar values and the electrochemical parameters. The display unit 120 is embedded in the housing and connected to the electrical signal processing unit, for displaying the detection value. The power supply mode of the electric signal processing unit is that the 5Vusb port supplies power, and the power can be supplied by the usb port of a common computer; and the charging port 150 may be used to communicate information with an external device connection.
The transient detection is that the spray pen is used to spray the body fluid sample to be detected onto the sensor surface fast in fixed speed, fixed amount and fast, and to collect instantaneous electric signal to obtain corresponding biological index concentration.
The principle of the detection system is as follows: under a certain electrode potential, when liquid flow containing an object to be detected (such as blood sugar, uric acid or vitamins and the like) is sprayed onto the surface of the electrode at a constant speed and in a quantitative manner, the object to be detected can instantaneously generate oxidation-reduction reaction on the surface of the electrode to generate an electric signal, and when the environmental conditions are consistent (the liquid flow spraying speed, the spraying dosage, the spraying distance and the concentration of the solution in the reagent bottle are kept unchanged), the electric signal value and the concentration of the object to be detected are in a linear relationship, so that quantitative analysis can be performed.
The enzyme-free kit 200 in the detection system is a disposable, portable and pre-packaged kit, and the enzyme-free three-electrode solid sensor 211 is utilized for detecting the concentration of biological indexes, so that the detection system is easy to use, simple to operate and capable of avoiding cross contamination; the method has the advantages of avoiding complex enzyme reaction steps in the traditional method and overcoming the defects of the microneedle wound detection method while realizing high sensitivity and convenience. The sample injection pen 300 in the detection system has a simple and small structure, can be used for sampling trace by a single hand, and can be used for rapidly, quantitatively and uniformly injecting and sampling at a constant speed so as to realize transient detection; compared with the existing static detection, the transient detection has high sensitivity and good reproducibility.
The following illustrates the differences between transient detection and static detection:
1) Minimum measurable concentration test: glucose standard with the concentration of 5umol/L is taken and measured by a static detection method and a transient detection method respectively, and the measurement is repeated for 3 times, and the result is shown in Table 1:
TABLE 1
2) Sample analysis linear test: glucose standard substances with concentrations of 5, 10, 20, 50, 100 and 200umol/L are respectively taken, the glucose standard substances are respectively measured by a static detection method and a transient detection method, each concentration is tested for 1 time, a linear regression equation is obtained by taking a theoretical concentration value as an independent variable and a current value as a dependent variable, a linear correlation coefficient r is calculated, and the results are shown in Table 2:
TABLE 2
3) Repeatability test: glucose standard with the concentration of 50umol/L is taken and measured by a static detection method and a transient detection method respectively, and the measurement is repeated for 10 times, and the result is shown in Table 3:
TABLE 3 Table 3
From the above test results, it can be seen that:
1) The minimum measurable concentration of the transient detection is 5umol/L and is smaller than the minimum measurable concentration of the static detection by 10umol/L, which indicates that the sensitivity of the transient detection is higher than that of the static detection.
2) The linear correlation coefficient of transient detection is 0.9978, which is larger than the linear correlation coefficient 0.9955 of static detection, indicating that the linear correlation of transient detection is better.
3) The reproducibility in transient detection is 3.88% and is less than 5.42% in static detection, which indicates that the reproducibility in transient detection is good.
The shape and structure of the high-sensitivity electrochemical detector 100 are described in detail below:
In an alternative embodiment of the present invention, the self-priming base 110 is equipped with a second magnetic element 112 that is magnetically opposite to the first magnetic element 216 of the bottle bottom 210.
Specifically, in the present embodiment, two second magnetic members 112 are provided, and correspondingly, two first magnetic members 216 are provided; because the first magnetic member 216 is magnetically opposite to the second magnetic member 112, the first magnetic member 216 is in attractive engagement with the second magnetic member 112, thereby allowing the bottle bottom 210 to be stably mounted within the self-priming base 110. Further, the first magnetic member 216 and the second magnetic member 112 are each configured as a magnetic iron pillar, and have equal diameters.
The first magnetic member 216 and the second magnetic member 112 are magnetically coupled to each other, so that the bottom of the enzyme-free kit 200 can be stably mounted on the high-sensitivity electrochemical detector 100.
In an alternative embodiment of the present invention, the bottle bottom 210 is provided with a first axial positioning structure, the self-priming base 110 is provided with a second rotational positioning structure, and the first axial positioning structure is matched with the second rotational positioning structure.
Specifically, the bottle bottom 210 is configured as a circle, and the first axial positioning structure is disposed on a sidewall of the bottle bottom 210 and is configured as a first positioning plane 217; the self-priming base 110 is correspondingly arranged to be round, the second rotary positioning structure is arranged on the inner wall of the self-priming base 110 and is arranged to be a second positioning plane 113, and the first positioning plane 217 is attached to the second positioning plane 113 to position the enzyme-free kit 200.
The first positioning plane 217 and the second positioning plane 113 avoid an error in the installation direction of the enzyme-free kit 200, and improve the installation efficiency.
In an alternative scheme of the embodiment of the invention, the high-sensitivity electrochemical detector 100 is arranged like a rectangle, and the upper surface is provided with a self-priming base 110, a display unit 120, a switch 130 and a switching key 140 at intervals; the sidewall is provided with a charging port 150. The whole structure is simple and small, and the use is convenient.
The structure and shape of the enzyme-free kit 200 are described in detail below:
in an alternative to the embodiment of the present invention, referring to fig. 4 to 9, the enzyme-free three-electrode solid sensor 211 includes a counter electrode 212, a working electrode 213, and a reference electrode 214; the counter electrode 212, the working electrode 213 and the reference electrode 214 are arranged at intervals.
Specifically, the enzyme-free kit 200 may detect the blood glucose concentration alone, the uric acid concentration alone, or both the blood glucose concentration and the uric acid concentration; of course, the kit can also detect other biological index concentrations. Still further, the bottle bottom 210 is provided as a circuit board substrate of a printed electrode, of course the bottle bottom 210 is not limited to the circuit board substrate; the circuit board is provided with an enzyme-free three-electrode solid sensor 211, and a three-electrode conductive contact 215 at the bottom of the bottle bottom 210 is used for connecting the high-sensitivity electrochemical detector 100 and outputting signals to the high-sensitivity electrochemical detector 100. In this embodiment, the thickness of the bottle bottom 210 is set to 1.6mm. Preferably, the body 220 is made of medical PP. The preparation method of the enzyme-free three-electrode solid sensor 211 comprises the following steps: first, the nano-particles with the proper size and surface modification are prepared, and then the nano-particles are combined with an electrochemical sensor to form the enzyme-free three-electrode solid sensor 211 capable of measuring blood sugar or uric acid. Further, the nanoparticles may be selected from metal nanoparticles (Au, ag, pt nanoparticles), carbon-based nanomaterials (graphene, carbon nanotubes, carbon dots, fullerenes), metal oxide semiconductor materials (black TiO2, fe3O 4), metal sulfides (MoS 2), etc.; modification of nanoparticles may be selected from: amino acid-mediated surface modification, thiol-compound-mediated surface modification, ligand exchange-based surface modification, and the like; or replacing the nanoparticles with an MXene material including Ti3C2, ti2C, nb2C, V2C, mo C, etc.; or magnetic particles or beads, such as silica gel, may be used to coat the beads. Of course, other materials are matched and modified to meet the requirement of excellent electrical performance, and the like, so long as the size meets the requirement, and the materials are also within the protection scope of the embodiment of the invention. In this embodiment, the working electrode 213 is made of conductive mixed carbon paste; the material of the counter electrode 212 is conductive carbon paste; the reference electrode 214 material is a conductive silver paste. Further, the counter electrode 212 is provided in an arc shape, the working electrode 213 and the reference electrode 214 are both provided in a circular shape, and the diameter of the working electrode 213 is larger than that of the reference electrode 214; of course, the shape and arrangement of the counter electrode 212, the working electrode 213, and the reference electrode 214 are not limited to the above-described forms, and other shapes and arrangements should be within the scope of the embodiments of the present invention.
The counter electrode 212, the working electrode 213 and the reference electrode 214 realize the redox action of the enzyme-free three-electrode solid sensor 211 on the human body fluid sample.
In an alternative embodiment of the present invention, the bottle body includes a bottle body 220 and a bottle cap 230, wherein one end of the bottle body 220 is connected to the bottle cap 230, and the other end is connected to the bottle bottom 210.
Specifically, referring to fig. 8, the body 220 is provided in a hollow cylindrical structure, and the cap 230 and the bottom 210 are respectively connected to both ends of the body 220.
In an alternative scheme of the embodiment of the invention, an installation protrusion 221 is arranged on the inner wall of the bottle body 220, and an installation groove 222 is arranged on the lower surface of the installation protrusion 221; the bottle bottom 210 is adhered to the lower surface of the mounting boss 221, and the first magnetic member 216 is inserted into the mounting groove 222 through the bottle bottom 210.
Specifically, referring to fig. 8, the mounting protrusion 221 protrudes toward the inside of the body 220; the first magnetic member 216 and the mounting groove 222 are provided with two.
The adhesion of the bottom 210 to the lower surface of the mounting protrusion 221 increases the contact area between the bottom 210 and the body 220, enhances the connection strength between the two, and prevents the bottom 210 from falling off the body 220. The two first magnetic pieces 216 are respectively inserted into the two mounting grooves 222, so that the first magnetic pieces 216 are connected with the bottle body 220.
In an alternative embodiment of the present invention, the bottle 220 is provided with a third axial positioning structure, and the first axial positioning structure is matched with the third axial positioning structure.
Specifically, the body 220 is configured as a cylinder, and the third axial positioning structure is disposed on the body 220 and is configured as a first positioning plane 217; the bottle bottom 210 is set to be circular, and the outer wall of the bottle bottom 210 is located to first axial location structure and is equipped with second locating plane 113, and first locating plane 217 laminating with third locating plane 223 realizes the positioning action to the bottle bottom 210, avoids bottle bottom 210 to be in the direction mistake when installing to the body 220.
The first and third positioning planes 217 and 223 prevent the bottom 210 from being misdirected when being mounted to the body 220, improving the mounting efficiency.
In an alternative embodiment of the present invention, the two mounting slots 222 are symmetrically disposed.
Specifically, the symmetry line of the two mounting slots 222 is perpendicular to the third positioning plane 223.
The two mounting grooves 222 are symmetrically arranged to realize the symmetrical arrangement of the two first magnetic members 216, which is helpful for stably mounting the bottom of the enzyme-free kit 200 on the high-sensitivity electrochemical detector 100.
In an alternative embodiment of the present invention, the cap 230 is screwed to the body 220.
Specifically, the bottle cap 230 is provided with external threads, the inner wall of the top of the bottle body 220 is provided with internal threads, and the external threads of the bottle cap 230 are in threaded connection with the internal threads of the bottle body 220.
The bottle cap 230 is screw-coupled with the body 220, and the bottle cap 230 is conveniently installed and removed.
In an alternative of the embodiment of the present invention, the bottle cap 230 includes an operation part 231 and a connection part 233, the operation part 231 is connected with the connection part 233, and the connection part 233 is in threaded connection with the bottle body 220; the outer wall of the operating part 231 is provided with an anti-slip structure 232.
Specifically, referring to fig. 9, the anti-slip structure 232 includes a plurality of grooves spaced apart along the circumferential direction of the operation portion 231, and each groove extends in the axial direction of the operation portion 231. Further, a sealing gasket is provided at the middle of the operation part 231, and the cap 230 is made of PVC material. The outer circumference of the connection part 233 is provided with a sealing ring to prevent liquid from leaking from the bottle cap 230.
The anti-slip structure 232 helps to increase friction between the bottle cap 230 and the operator's hand when the bottle cap 230 is mounted or dismounted, thereby facilitating unscrewing or tightening of the bottle cap 230.
In an alternative embodiment of the present invention, a centralizer 240 is mounted within the body 220.
Specifically, the centralizer 240 is fixed to the middle of the body 220, and the axis of the centralizer 240 is collinear with the axis of the body 220. Preferably, the centralizer 240 is made of medical PP material.
The centralizer 240 serves to accurately center the sample injection pen 300 after it is inserted into the body 220.
The structure and shape of the sample injection pen 300 are described in detail below:
In an alternative to the embodiment of the present invention, referring to fig. 10 to 12, the control mechanism includes a push switch lever 330, a liquid injection spring 340, a piston rod 350, and a piston sleeve 360; the push switch lever 330 is mounted on the top of the housing 310, and one end thereof extends into the housing 310, and the piston sleeve 360 is mounted in the housing 310 and spaced apart from the push switch lever 330; the bottom of push switch rod 330 is equipped with spout 331, and penetrate liquid spring 340 and be located spout 331, and the one end of piston rod 350 is connected with penetrating liquid spring 340 and with spout 331 sliding fit, piston sleeve 360 cover locate piston rod 350, and the end is installed fixed speed ration plunger mechanism 320. The control mechanism further comprises a return spring 370 and an outer sleeve 380, wherein the outer sleeve 380 is positioned in the housing 310 and sleeved on the piston sleeve 360; one end of the piston rod 350 extending into the sliding groove 331 is provided with a protrusion 351, the outer sleeve 380 is provided with a 7-shaped limit groove 381, and the protrusion 351 is inserted into the limit groove 381; one end of the return spring 370 abuts against the stopper protrusion 332 of the push switch lever 330, and the other end abuts against the outer sleeve 380.
Specifically, the outer wall of the push switch lever 330 is provided with a limit protrusion 332, and the bottom is provided with a chute 331. The push switch lever 330 has a liquid-absorbing or liquid-spraying switching function, and when the push switch lever 330 is pushed down for the first time and lifted up, a sample is sucked into the sample injection pen 300, and when the push switch lever 330 is pushed down for the second time, the sample is sprayed and covered on the electrode plate rapidly, at a constant speed and uniformly through the constant-speed and constant-quantity plunger mechanism 320. Quantitative control of the amount of suction can be achieved by modifying the inner diameter of the piston rod 350 and the piston sleeve 360 that mates with the piston rod 350. Quantitative control of the injection quantity can be achieved by changing the size of the limit groove 381. The injection speed is determined by the elastic potential energy of the injection spring 340, and the injection speed of the sample can be changed by changing the elastic potential energy of the injection speed. The housing 310 is provided as a pen for ease of use, portability and storage.
The sample injection pen 300 achieves the effects of micro-quantification, constant speed and rapid injection while achieving the function of injecting biological samples. And provides a guarantee for the consistency of the detection amount of the extracted biological samples.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (10)
1. A human body fluid-based biological indicator concentration transient detection system, comprising: a high-sensitivity electrochemical detector (100), an enzyme-free kit (200) and a sample injection pen (300);
The enzyme-free kit (200) comprises a bottle body and a bottle bottom (210), wherein the bottle bottom (210) is arranged at the bottom of the bottle body, an enzyme-free three-electrode solid sensor (211) is arranged on the surface, close to the bottle body, of the bottle body, and a three-electrode conductive contact (215) is arranged on the other surface of the bottle body;
The high-sensitivity electrochemical detector (100) is provided with a self-priming base (110), the self-priming base (110) is provided with a three-electrode elastic contact (111), the self-priming base (110) is in limit fit with the enzyme-free kit (200), and the three-electrode elastic contact (111) is connected with the three-electrode conductive contact (215);
The sample injection pen (300) comprises a shell (310), a control mechanism and a constant-speed and quantitative plunger mechanism (320), wherein the control mechanism is arranged on the shell (310) and is used for controlling the sample injection pen (300) to quantitatively absorb or inject a human body fluid sample to the surface of the enzyme-free three-electrode solid sensor (211) at a constant speed, and the high-sensitivity electrochemical detector (100) collects instantaneous electric signals so as to detect the concentration of biological indexes; the constant-speed and quantitative plunger mechanism (320) is mounted at the tail end of the sample injection pen (300).
2. The human body fluid based bio-index concentration transient detection system of claim 1, wherein the enzyme-free three-electrode solid state sensor (211) comprises a counter electrode (212), a working electrode (213) and a reference electrode (214);
The counter electrode (212), the working electrode (213) and the reference electrode (214) are arranged at intervals.
3. The human body fluid-based biological indicator concentration transient detection system of claim 2, wherein the working electrode (213) material is a conductive mixed carbon paste; the material of the counter electrode (212) is conductive carbon paste; the reference electrode (214) is made of conductive silver paste.
4. The human body fluid-based biological indicator concentration transient detection system of claim 1, wherein the bottle bottom (210) is provided with a first magnetic element (216), the self-priming base (110) is provided with a second magnetic element (112), and the first magnetic element (216) is magnetically opposite to the second magnetic element (112).
5. The human body fluid-based biological indicator concentration transient detection system of claim 1, wherein the bottle bottom (210) is provided with a first axial positioning structure, the self-priming base (110) is provided with a second rotational positioning structure, and the first axial positioning structure is matched with the second rotational positioning structure.
6. The human body fluid based bio-indicator concentration transient detection system of claim 1, wherein the control mechanism comprises a push switch lever (330), a fluid ejection spring (340), a piston rod (350), and a piston sleeve (360);
The push switch rod (330) is arranged at the top of the shell (310), one end of the push switch rod extends into the shell (310), and the piston sleeve (360) is arranged in the shell (310) and is arranged at intervals with the push switch rod (330);
The bottom of push switch pole (330) is equipped with spout (331), penetrate liquid spring (340) and be located in spout (331), one end of piston rod (350) with penetrate liquid spring (340) be connected and with spout (331) sliding fit, piston sleeve (360) cover is located piston rod (350), and the end is installed fixed speed ration plunger mechanism (320).
7. The human body fluid-based biological indicator concentration transient detection system of claim 6, wherein the control mechanism further comprises a return spring (370) and an outer sleeve (380), the outer sleeve (380) being located within the housing (310) and being sleeved on a piston sleeve (360);
One end of the piston rod (350) extending into the sliding groove (331) is provided with a protrusion (351), the outer sleeve (380) is provided with a 7-shaped limit groove (381), and the protrusion (351) is inserted into the limit groove (381);
One end of the return spring (370) is abutted with the limit protrusion (332) of the push switch lever (330), and the other end is abutted with the outer sleeve (380).
8. The human body fluid-based biological indicator concentration transient detection system according to claim 1, wherein the bottle body comprises a bottle body (220) and a bottle cap (230), one end of the bottle body (220) is connected with the bottle cap (230), and the other end is connected with the bottle bottom (210).
9. The human body fluid-based biological index concentration transient detection system according to claim 8, wherein an inner wall of the bottle body (220) is provided with a mounting protrusion (221), and a lower surface of the mounting protrusion (221) is provided with a mounting groove (222);
the bottle bottom (210) is adhered to the lower surface of the mounting protrusion (221), and the first magnetic member (216) is inserted into the mounting groove (222) through the bottle bottom (210).
10. The human body fluid-based biological indicator concentration transient detection system of claim 8, wherein the body (220) is provided with a third axial positioning structure, the first axial positioning structure cooperating with the third axial positioning structure.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410405992.XA CN118191070A (en) | 2024-04-07 | 2024-04-07 | Biological index concentration transient detection system based on human body fluid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410405992.XA CN118191070A (en) | 2024-04-07 | 2024-04-07 | Biological index concentration transient detection system based on human body fluid |
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| CN118191070A true CN118191070A (en) | 2024-06-14 |
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| CN202410405992.XA Pending CN118191070A (en) | 2024-04-07 | 2024-04-07 | Biological index concentration transient detection system based on human body fluid |
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| CN (1) | CN118191070A (en) |
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- 2024-04-07 CN CN202410405992.XA patent/CN118191070A/en active Pending
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