CN118179619A - Microfluidic slide and microfluidic slide clamp - Google Patents

Abstract

The invention discloses a microfluidic slide and a microfluidic slide clamp, wherein the microfluidic slide comprises a main body with a microfluidic fluid channel, and a sample injection cup 11 and a liquid drop collecting cup which are respectively connected with the main body; the sample injection cup is communicated with the liquid drop collecting cup through the microfluidic fluid channel; a spacer extending in a fluid direction within the microfluidic fluid channel is disposed within the microfluidic fluid channel. According to the invention, the separator is arranged in the microfluidic fluid channel, so that the blocking impurities in the microfluidic fluid channel are difficult to block the microfluidic slide, the quality of generated liquid drops is improved, and meanwhile, the internal damage rate of the microfluidic fluid channel is reduced.

Description

Microfluidic slide and microfluidic slide clamp

Technical Field

The disclosure relates to the technical field of microfluidics, in particular to a microfluidic slide and a microfluidic slide clamp.

Background

The droplet microfluidic technology presents obvious advantages in the single cell field, namely low reagent consumption, and can generate nano-liter or even picoliter droplets; and secondly, independent reaction space can be provided for single cells so as to reduce cross contamination. Therefore, the droplet microfluidic technology has important application in biology and biomedicine. An indispensable device in droplet microfluidic technology is a microfluidic slide. However, due to the special structure of the microfluidic slide, defects of reagent blocking impurities blocking the microfluidic slide often occur at the position of the microfluidic slide where the channel is narrower. When a microfluidic slide becomes clogged, the quality of the generated droplets will be affected and the internal channels of the microfluidic fluidic channels will be damaged.

Disclosure of Invention

The invention aims to overcome the defects that the blocking of a microfluidic slide in the prior art affects the quality of generated liquid drops and damages an internal channel of the microfluidic slide, and provides the microfluidic slide and a microfluidic slide clamp.

The invention solves the technical problems by the following technical scheme:

In a first aspect, the present invention provides a microfluidic slide comprising a body having a microfluidic fluidic channel, and a sample introduction cup and a droplet collection cup respectively connected to the body;

The sample introduction cup is communicated with the liquid drop collecting cup through the microfluidic fluid channel;

and a spacer extending along the fluid direction in the microfluidic fluid channel is arranged in the microfluidic fluid channel.

Optionally, the shape of the spacer is a bar.

Optionally, the number of the spacers is plural, and the plural spacers are disposed at predetermined intervals along the fluid direction.

Optionally, the spacer is disposed on an inner wall of the microfluidic fluid channel and is convex.

Optionally, the sample introduction cup comprises a continuous phase sample introduction cup, and the separator is disposed within a branch conduit of the microfluidic fluid channel in communication with the continuous phase sample introduction cup.

Optionally, the sample feeding cup and the liquid drop collecting cup comprise an upper cup body and a lower cup body, the upper cup body is provided with an opening facing to the outside of the microfluidic slide, and the lower cup body is connected between the upper cup body and the microfluidic fluid channel;

the upper cup body is connected with the lower cup body through a round corner part;

and/or the lower cup body is connected with the microfluidic fluid channel through a round corner part.

Optionally, the sample injection cup comprises an injection port communicated with the outside of the microfluidic slide and a sample outlet connected between the injection port and the microfluidic fluid channel, and the aperture of the injection port monotonically decreases along the direction towards the sample outlet;

And/or the liquid drop collecting cup comprises a connecting port connected with one end of the microfluidic fluid channel far away from the sample injection cup and a collecting port communicated with the outside of the microfluidic slide, wherein the connecting port is connected between the microfluidic fluid channel and the collecting port, and the aperture of the collecting port monotonically increases along the direction far away from the connecting port.

Optionally, the sample introduction cup further comprises at least one disperse phase sample introduction cup spaced between the droplet collection cup and the continuous phase sample introduction cup; the microfluidic fluid channel comprises a first branch pipeline connected with the continuous phase sample sampling cup, a second branch pipeline communicated with the disperse phase sample sampling cup, and a liquid drop generating pipeline communicated with the first branch pipeline and the second branch pipeline; the first branch pipeline and one end of the second branch pipeline, which is far away from the sample introduction cup, are intersected in a liquid drop generation area, and the liquid drop generation pipeline is connected between the liquid drop generation area and the liquid drop collection cup.

Optionally, the first branch pipes are symmetrically arranged on opposite sides of the second branch pipe and surround the second branch pipe.

Optionally, the main body is further provided with a counter bore connected between the sample outlet and the microfluidic fluid channel, and the aperture of the counter bore is larger than the aperture of one end of the sample outlet, which is close to the counter bore. In a second aspect, the present invention provides a microfluidic slide holder, comprising: a bracket with a clamping groove and a cover plate;

The clamping groove is used for bearing any microfluidic slide;

a sealing gasket is arranged on one side, close to the clamping groove, of the cover plate and is used for sealing a collecting opening of the liquid drop collecting cup of the microfluidic slide, and an opening is formed in the middle of the sealing gasket and communicated with the collecting opening;

The cover plate is provided with a plate hole which is communicated with the open hole;

the plate holes are used for being externally connected with a negative pressure device.

Optionally, the plate hole is connected with a negative pressure device through a dispensing needle, and the negative pressure device is used for providing a pressure difference between a sample outlet of the sample introduction cup and a sample introduction port of the liquid drop collecting cup.

Optionally, an observation port communicated with the clamping groove is formed in one side, away from the clamping groove, of the support, the microfluidic slide mounted in the clamping groove at least partially covers the observation port, and one side, close to the observation port, of the microfluidic slide is made of transparent materials; the observation port is used for docking the optical detection device.

The invention has the beneficial effects that: by arranging the separator in the microfluidic fluid channel, the blocking impurities in the microfluidic fluid channel are difficult to block the microfluidic slide, the quality of generated liquid drops is improved, and meanwhile, the internal damage rate of the microfluidic fluid channel is reduced.

Drawings

Fig. 1 is a schematic diagram of an assembly structure of a microfluidic chip and a fixture thereof according to an embodiment of the present invention;

Fig. 2 is a top view of an assembly structure of a microfluidic chip and a fixture thereof according to an embodiment of the present invention;

FIG. 3 is a cross-sectional view taken along line A-A of the microfluidic slide of FIG. 2 assembled with a fixture;

Fig. 4 is an exploded view of the assembly structure of the microfluidic slide and the fixture shown in fig. 2;

Fig. 5 is a top view of a microfluidic slide according to an embodiment of the present invention;

fig. 6 is an enlarged schematic view of a portion of the structure of the microfluidic slide of fig. 5;

FIG. 7 is a schematic diagram of a microfluidic fluidic channel of the microfluidic slide of FIG. 5;

FIG. 8 is a cross-sectional view of the microfluidic slide of FIG. 5 taken along line B-B;

FIG. 9 is an enlarged schematic cross-sectional view of the microfluidic slide of FIG. 5 taken along line C-C;

Fig. 10 is a schematic diagram of a fixture for mating with a microfluidic slide according to an embodiment of the present invention.

Detailed Description

The invention is further illustrated by means of the following examples, which are not intended to limit the scope of the invention.

The present invention provides a microfluidic slide 1, see fig. 1, 3, 6 and 7, the microfluidic slide 1 comprising: a main body 10 having a microfluidic fluid channel 100, and a sample introduction cup 11 and a droplet collection cup 12 connected to the main body 10, respectively. The sample introduction cup 11 communicates with the droplet collection cup 12 through the microfluidic fluid channel 100. The microfluidic fluid channel is provided therein with spacers 3 extending in the direction of the fluid in the microfluidic fluid channel.

The microfluidic slide 1 may be a hard microfluidic slide or a soft microfluidic slide. In the present application, a hard microfluidic chip such as a polymer chip, a glass chip, a silicon substrate chip, etc. is mainly used. The number of sample introduction cups 11 is not limited to 3 shown in the figure, but may be 1,2, 4 or more. It will be appreciated by those skilled in the art that the size and shape of each sample introduction cup 11 may be the same or different. Referring to fig. 2, three sample cups 11 of the figure are shown with larger dimensions for the oil phase and the remaining two smaller dimensions for the continuous phase sample. The height of the drip-collecting cup 12 does not have to be identical to the height of the sample introduction cup 11. Referring to fig. 3-7, a sample introduction cup 11 communicates with a droplet collection cup 12 through a microfluidic fluid channel 100 in a body 10, and a spacer 3 is disposed within the microfluidic fluid channel 100. The material of the spacer 3 may be selected according to the requirement, and may be a metal material, and the selected material of the spacer 3 does not physically/chemically react with the sample or the oil phase in the microfluidic fluid channel 100. Referring to fig. 8, the microfluidic fluid channel 100 is formed at the bottom of the main body 10, and the bottom of the main body 10 is covered with the film 13 to seal the microfluidic fluid channel 100. The microfluidic fluidic channel 100 is bonded to the membrane layer 13 in two general ways: one is to bond the microfluidic fluidic channel 100 and the membrane layer 13 by a glue layer (such as PSA, etc.); and secondly, bonding the microfluidic fluid channel 100 and the membrane layer 13 through a thermocompression bonding process. In addition, the embodiment of the application has no specific requirements on the appearance, the size, the material and the shape and the size of the micro-flow channel of the microfluidic slide 1, so that the droplet generation function can be realized.

In this embodiment, by providing the spacer 3 in the microfluidic fluid channel 100, the size of the fluid flowing in the microfluidic fluid channel 100 can be limited, that is, the middle in the microfluidic fluid channel 100 is properly blocked by the spacer 3 to introduce air or external impurities, so as to prevent the air or impurities from directly flowing into the narrowest part in the microfluidic fluid channel 100 to block the microfluidic slide 1. And the spacer 3 can also effectively prevent the channel collapse of the microfluidic fluid channel 100.

Preferably, as shown in fig. 6, the spacer 3 has a strip shape.

The shape of the spacer 3 is a strip, so that air or external impurities introduced through the spacer 3 in the middle of the microfluidic fluid channel 100 can be effectively blocked, the quality of generated liquid drops is improved, and meanwhile, the damage rate of the microfluidic fluid channel 100 is reduced.

The spacers 3 may be continuous strips as shown in fig. 6, or a plurality of spacers 3 may be provided so as to extend at intervals, and the plurality of spacers 3 may extend in the same direction as the fluid and preferably at equal intervals.

Wherein the flow direction of the air or the impurities flowing into the microfluidic fluid channel 100 is difficult to judge, and in some cases, one separator 3 is difficult to effectively block more air or impurities, and does not cause clogging or damage in a short time, but is easily caused under a long time of use. Therefore, the plurality of spacers 3 are provided, and the plurality of spacers 3 are provided at predetermined intervals in the fluid direction, so that more air or impurities can be effectively flowed into the narrowest place in the microfluidic fluid channel 100 to block the microfluidic chip 1. The quality of the generated droplets is improved while the damage rate of the microfluidic fluid channel 100 is reduced.

Preferably, the spacer 3 is provided on the inner wall of the microfluidic fluid channel 100 in a convex shape.

The convex separator 3 is disposed on the inner wall of the microfluidic fluid channel 100 to block air or external impurities properly, thereby improving the quality of droplets and reducing the damage rate of the microfluidic fluid channel 100.

In one embodiment, the sample introduction cup 11 comprises a continuous phase sample introduction cup 11a, and the separator 3 is disposed within a branch conduit 100a of the microfluidic fluid channel 100 in communication with the continuous phase sample introduction cup 11 a.

For example, referring to fig. 2 and 5, continuous phase sample introduction cup 11a is used to hold a continuous phase liquid, preferably an oil phase, dispersed phase sample introduction cup 11b is used to hold a cell liquid or microbead liquid, and drop collection cup 12 is used to collect the generated drops. The oil phase reagent has the defect of easily blocking the slide glass due to the blocking impurities, and therefore, the separator 3 is arranged in the branch pipeline of the microfluidic fluid channel 100 connected with the continuous phase sample introduction cup 11a for containing the oil phase.

In addition, the tubing between the three-stream junction of sample introduction cup 11 and drop collection cup 12 may be designed as a serpentine shape.

In this embodiment, the substance (oil phase) contained in the continuous phase sample introduction cup 11a is easy to block, and the microfluidic channel 100 that is easy to block is properly blocked by the separator 3 from air or external impurities, so that the quality of the generated droplets is improved, and the damage rate of the microfluidic channel 100 is reduced.

In one embodiment, referring to fig. 8 and 9, the sample introduction cup 11 and the droplet collection cup 12 each include an upper cup (111, 121) having an opening toward the outside of the microfluidic slide 1 and a lower cup (112, 122) connected between the upper cup and the microfluidic fluid channel, the upper cup being connected to the lower cup by a rounded corner 113; and/or the lower cup is also smoothly connected with the microfluidic fluid channel 10 by chamfering.

Wherein, the upper cup body and the lower cup body can be inverted hollow round tables, the taper of the upper cup body can be designed to be 1-30 degrees, the taper of the lower cup body can be designed to be 30-150 degrees, and the joint of the upper cup body and the lower cup body is provided with a round corner part 113. In addition, the lower cup may be connected to the microfluidic fluid channel 100 through a rounded corner 113.

In this embodiment, the rounded corner 113 is provided at the joint surface, so that the liquid residue in the sample introduction cup 11 can be reduced, the reaction reagents contained in the sample introduction cup 11 can be fully reacted, and the quality of the droplets generated by the droplet collecting cup 12 can be improved.

In one embodiment, the sample introduction cup 11 includes a sample introduction port 110 in communication with the outside of the microfluidic slide 1 and a sample outlet 114 connected between the sample introduction port 110 and the microfluidic fluid channel, the aperture of the sample introduction port 110 of the sample introduction cup 11 monotonically decreases in a direction toward the sample outlet 114, and/or the droplet collection cup 12 includes a connection port 124 connected at an end of the microfluidic fluid channel remote from the sample introduction cup 11 and a collection port 120 in communication with the outside of the microfluidic slide 1, the connection port 124 being connected between the microfluidic fluid channel and the collection port 120, the aperture of the collection port 120 monotonically increasing in a direction away from the connection port 124.

The shape of the upper cup body and the lower cup body of the sample feeding cup 11 may be an inverted hollow circular truncated cone, or may be other shapes, which only needs to satisfy that the aperture of the sample feeding port 110 of the sample feeding cup 11 monotonically decreases along the direction towards the sample outlet 114. The sample introduction cup 11 includes a sample introduction port 110 communicating with the outside of the microfluidic slide 1, the sample introduction port 110 receiving an externally inflowing dispersed phase (e.g., aqueous phase) or continuous phase (e.g., oil phase) sample, and a sample outlet 114 connected between the sample introduction port 110 and the microfluidic fluid channel, the sample outlet 114 for outflowing the dispersed phase sample and connecting to the microfluidic fluid channel 100. The droplet collecting cup 12 comprises a connecting port 124 connected to the end of the microfluidic fluid channel far from the sample introduction cup 11 and a collecting port 120 communicated with the outside of the microfluidic slide 1, the connecting port 124 is used for communicating with the microfluidic fluid channel, and the connecting port 124 is connected between the microfluidic fluid channel and the collecting port 120. The collection port 120 is for flowing out the droplets to be collected, and the aperture of the collection port 120 increases monotonically in a direction away from the connection port 124, for example, in a hollow truncated cone shape.

In this embodiment, by limiting the tendency of the aperture of the sample inlet 110 of the sample cup 11 to monotonically decrease in the direction toward the sample outlet 114 and/or the aperture of the limited purchase set of openings 120 to monotonically increase in the direction away from the connection port 124, the liquid residue in the sample cup 11 can be reduced, allowing all of the reagents contained in the sample cup 11 to react, improving the quality of the droplets produced by the droplet collection cup 12 and facilitating the collection of the droplets.

In one embodiment, sample introduction cup 11 further comprises at least one dispersed phase sample introduction cup 11b disposed in spaced relation between droplet collection cup 12 and continuous phase sample introduction cup 11 a; as shown in fig. 5to 6, two disperse phase sample introduction cups 11b are provided on the slide 1, and the microfluidic fluid channel includes a first branch pipe 100a connected to the continuous phase sample introduction cup 11a, a second branch pipe 100b communicating with the disperse phase sample introduction cup 11b, and a droplet generation pipe 100c communicating with the first branch pipe 100a and the second branch pipe 100 b; the ends of the first branch conduit 100a and the second branch conduit 100b remote from the sample cup 11a/b meet at a droplet generation region P, and a droplet generation conduit 100c is connected between the droplet generation region P and the droplet collection cup 12. The first branch pipes 100a are symmetrically disposed at opposite sides of the second branch pipe 100b and surround the second branch pipe 100b.

Referring to fig. 2 and 5-6, there are shown a continuous phase sample introduction cup 11a and a disperse phase sample introduction cup 11b, wherein the oil phase liquid flows into the first branch pipe 100a through the continuous phase sample introduction cup 11a, the aqueous phase liquid flows into the second branch pipe 100b through the disperse phase sample introduction cup 11b, finally, the aqueous phase liquid is combined in the droplet generation region P, and the oil phase liquid wraps the aqueous phase sample and is sheared into droplets in the droplet generation region P. Preferably, the first branch pipes 100a are symmetrically disposed at opposite sides of the second branch pipe 100b and surround the second branch pipe 100b.

In one embodiment, referring to fig. 5, the body 10 is further provided with a counterbore 101 connected between the sample outlet 114 and the microfluidic fluid channel, the bore diameter of the counterbore 101 being larger than the bore diameter of the end of the sample outlet 114 adjacent the counterbore 101.

In this embodiment, the design can effectively fully flow the liquid contained in the sample introduction cup 11 into the flow channel, and promote the stability of the contained reaction reagent.

In one embodiment, referring to fig. 1, 3, 4 and 10, a microfluidic slide clamp 2 comprises: a bracket 20 with a clamping groove 200 and a cover plate 21, wherein the clamping groove 200 is used for bearing the microfluidic slide 1. The cover plate 21 is provided with a sealing gasket 22 near one side of the clamping groove 200, the sealing gasket 22 is used for sealing the collecting opening 120 of the liquid drop collecting cup 12 of the microfluidic slide 1, and an opening 220 is arranged in the middle of the sealing gasket 22 and communicated with the collecting opening 120. The cover 21 is provided with a plate hole 210, the plate hole 210 is aligned with the opening 220, and the plate hole 210 is used for externally connecting a negative pressure device.

Wherein, when the microfluidic slide 1 is used, it is placed on the card slot 200 for fixing the microfluidic slide 1. The collection port 120 of the drip-collecting cup 12 is preferably smooth and flat to facilitate the use of the gasket 22 to seal the collection port 120 of the drip-collecting cup 12 of the microfluidic slide 1, ensuring good air tightness. An opening 220 (which may be a hole with a diameter of 1.5 mm) is arranged in the middle of the sealing pad 22 (made of silica gel, etc.), the opening 220 is aligned with a plate hole 210 on the cover plate 21, nuts on the left and right sides of the microfluidic slide clamp 2 are screwed down by screws 26a, and the nuts are fixed with the buckles 26b, so that the microfluidic slide 1 can be sealed. After sealing is completed, plate well 210 is externally connected to a negative pressure device (peristaltic pump, syringe, negative pressure pump, etc.).

Furthermore, it should be understood by those skilled in the art that the number of microfluidic slide holders 2 that can carry a microfluidic slide 1 is not limited, and that one microfluidic slide 1 may be carried, or a plurality of microfluidic slides 1 may be carried.

Preferably, referring to fig. 2,3, 4 and 10, plate hole 210 is connected by a dispensing needle 23 to a negative pressure device for providing a pressure differential between sample outlet 114 of sample introduction cup 11 and connection port 124 of drip collection cup 12.

After the negative pressure device is started, a pressure difference between the sample outlet 114 of the sample introduction cup 11 and the connection port 124 of the droplet collecting cup 12 can be provided, and a shearing force is generated on the water phase in the droplet generation region P to generate droplets. In addition, by adjusting the parameters of the negative pressure device, the speed and volume of droplet generation are adjusted.

In this embodiment, the microfluidic chip 1 is placed on the clamping groove 200 to fix and seal the collection port 120 of the droplet collection cup 12, and the plate hole 210 is externally connected with a negative pressure device, so that droplets can be directly generated in the droplet generation region P with shearing force, no special reagent is required to be used for sealing or sucking out, no additional operation is required, and the use is convenient. The speed and volume of droplet generation can also be adjusted by adjusting the negative pressure device.

As shown in fig. 3, in one embodiment, an observation port 201 communicating with the card slot 200 is provided on a side of the bracket 20 away from the card slot 200, the microfluidic chip 1 mounted on the card slot 200 at least partially covers the observation port 201, and a side of the microfluidic chip 1 close to the observation port 201 is made of transparent material; the viewing port 201 is used to interface with an optical detection device.

Wherein a microscope is used to observe the droplet generation process of the microfluidic slide 1. When the droplet generation process fails due to a specific cause (such as a blockage of a foreign matter in the microfluidic fluid channel 100), the droplet generation of the microfluidic slide 1 can be stopped in time, and the reaction reagent is saved.

In the present embodiment, the flow state of the fluid in the microfluidic slide 1 and the investigation of the droplet generation condition can be observed under a microscope. The microfluidic slide 1 and the clamp thereof can be matched with a common inverted or upright microscope in a laboratory without customizing a special microscope. The observation and analysis of the droplet generation reaction can be performed in real time, and the droplet generation state can be observed.

While specific embodiments of the invention have been described above, it will be appreciated by those skilled in the art that this is by way of example only, and the scope of the invention is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the principles and spirit of the invention, but such changes and modifications fall within the scope of the invention.

Claims (13)

1. The microfluidic slide is characterized by comprising a main body with a microfluidic fluid channel, and a sample injection cup and a liquid drop collecting cup which are respectively connected with the main body;

The sample introduction cup is communicated with the liquid drop collecting cup through the microfluidic fluid channel;

and a spacer extending along the fluid direction in the microfluidic fluid channel is arranged in the microfluidic fluid channel.

2. The microfluidic slide of claim 1, wherein the spacer is in the shape of a bar.

3. The microfluidic slide of claim 1, wherein the number of spacers is a plurality, the plurality of spacers being arranged at predetermined intervals along the fluid direction.

4. The microfluidic slide of claim 1, wherein the spacer is disposed on an inner wall of the microfluidic fluid channel and is convex.

5. The microfluidic slide of claim 1, wherein the sample introduction cup comprises a continuous phase sample introduction cup, the spacer being disposed within a branch conduit of a microfluidic fluid channel in communication with the continuous phase sample introduction cup.

6. The microfluidic slide of claim 1, wherein the sample introduction cup and the droplet collection cup each comprise an upper cup having an opening toward the outside of the microfluidic slide and a lower cup connected between the upper cup and the microfluidic fluid channel;

the upper cup body is connected with the lower cup body through a round corner part;

and/or the lower cup body is connected with the microfluidic fluid channel through a round corner part.

7. The microfluidic slide of claim 1, wherein the sample introduction cup comprises an introduction port in communication with the exterior of the microfluidic slide and a sample outlet connected between the introduction port and the microfluidic fluid channel, the aperture of the introduction port monotonically decreasing in a direction toward the sample outlet;

And/or the liquid drop collecting cup comprises a connecting port connected with one end of the microfluidic fluid channel far away from the sample injection cup and a collecting port communicated with the outside of the microfluidic slide, wherein the connecting port is connected between the microfluidic fluid channel and the collecting port, and the aperture of the collecting port monotonically increases along the direction far away from the connecting port.

8. The microfluidic slide of claim 5, wherein the sample introduction cup further comprises at least one dispersed phase sample introduction cup spaced between the drop collection cup and the continuous phase sample introduction cup; the microfluidic fluid channel comprises a first branch pipeline connected with the continuous phase sample sampling cup, a second branch pipeline communicated with the disperse phase sample sampling cup, and a liquid drop generating pipeline communicated with the first branch pipeline and the second branch pipeline; the first branch pipeline and one end of the second branch pipeline, which is far away from the sample introduction cup, are intersected in a liquid drop generation area, and the liquid drop generation pipeline is connected between the liquid drop generation area and the liquid drop collection cup.

9. The microfluidic slide of claim 8, wherein the first branch conduit is symmetrically disposed on opposite sides of and surrounds the second branch conduit.

10. The microfluidic slide of claim 7, wherein the body is further provided with a counterbore connected between the sample outlet and the microfluidic fluid channel, the counterbore having a larger pore size than an end of the sample outlet adjacent the counterbore.

11. A microfluidic slide clamp, the microfluidic slide clamp comprising: a bracket with a clamping groove and a cover plate;

The clamping groove is used for bearing the microfluidic slide according to any one of claims 1-9;

a sealing gasket is arranged on one side, close to the clamping groove, of the cover plate and is used for sealing a collecting opening of the liquid drop collecting cup of the microfluidic slide, and an opening is formed in the middle of the sealing gasket and communicated with the collecting opening;

The cover plate is provided with a plate hole which is communicated with the open hole;

the plate holes are used for being externally connected with a negative pressure device.

12. The microfluidic slide clamp of claim 11, wherein the plate holes are connected by dispensing needles to a negative pressure device for providing a pressure differential between the sample outlet 114 of the sample introduction cup and the connection port of the droplet collection cup.

13. The microfluidic slide clamp according to claim 11 or 12, wherein an observation port communicated with the clamping groove is formed in one side of the bracket away from the clamping groove, the microfluidic slide mounted in the clamping groove at least partially covers the observation port, and one side of the microfluidic slide close to the observation port is made of transparent material; the observation port is used for docking the optical detection device.