CN118127178A - SNP marker related to antiviral property of micropterus salmoides and application thereof - Google Patents
SNP marker related to antiviral property of micropterus salmoides and application thereof Download PDFInfo
- Publication number
- CN118127178A CN118127178A CN202410311574.4A CN202410311574A CN118127178A CN 118127178 A CN118127178 A CN 118127178A CN 202410311574 A CN202410311574 A CN 202410311574A CN 118127178 A CN118127178 A CN 118127178A
- Authority
- CN
- China
- Prior art keywords
- snp
- micropterus salmoides
- molecular marker
- sequence
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001125889 Micropterus salmoides Species 0.000 title claims abstract description 32
- 239000003550 marker Substances 0.000 title abstract description 9
- 230000000840 anti-viral effect Effects 0.000 title abstract description 8
- 238000012216 screening Methods 0.000 claims abstract description 16
- 239000003147 molecular marker Substances 0.000 claims abstract description 14
- 230000002924 anti-infective effect Effects 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims description 13
- 238000002844 melting Methods 0.000 claims description 5
- 230000008018 melting Effects 0.000 claims description 5
- 238000012408 PCR amplification Methods 0.000 claims description 4
- 238000012163 sequencing technique Methods 0.000 claims description 3
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 2
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 13
- 238000009395 breeding Methods 0.000 abstract description 11
- 230000001488 breeding effect Effects 0.000 abstract description 11
- 208000035240 Disease Resistance Diseases 0.000 abstract description 7
- 208000015181 infectious disease Diseases 0.000 abstract description 6
- 230000003612 virological effect Effects 0.000 abstract description 5
- 241000321429 Epinephelus itajara Species 0.000 abstract description 4
- 238000010219 correlation analysis Methods 0.000 abstract description 3
- 238000012214 genetic breeding Methods 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 238000011161 development Methods 0.000 abstract description 2
- 241000700605 Viruses Species 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 5
- 241000813323 Maize streak Reunion virus Species 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000251468 Actinopterygii Species 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 4
- 241000711804 Infectious hematopoietic necrosis virus Species 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241001505329 Lymphocystis disease virus 1 Species 0.000 description 2
- 108091092878 Microsatellite Proteins 0.000 description 2
- 241000961010 Miichthys miiuy Species 0.000 description 2
- 241000269908 Platichthys flesus Species 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 238000000546 chi-square test Methods 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 238000011880 melting curve analysis Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 238000000197 pyrolysis Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 101150025032 13 gene Proteins 0.000 description 1
- 241000628923 Anadara sativa Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 101150027068 DEGS1 gene Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000701372 Iridovirus Species 0.000 description 1
- 241000269794 Lateolabrax Species 0.000 description 1
- 241001327682 Oncorhynchus mykiss irideus Species 0.000 description 1
- 241000269799 Perca fluviatilis Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 210000004690 animal fin Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229940096422 collagen type i Drugs 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 208000022602 disease susceptibility Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 208000010824 fish disease Diseases 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 230000008799 immune stress Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 231100000636 lethal dose Toxicity 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides an SNP marker related to the antiviral property of micropterus salmoides and application thereof, wherein the SNP molecular marker related to the rhabdovirus resistance of micropterus salmoides is positioned at the 201 st position of the sequence of SEQ ID NO. 1 and is replaced by T/C. According to the invention, the molecular marker is utilized to perform disease resistance correlation analysis, and a significant SNP locus related to the resistance to rhabdovirus infection of the micropterus salmoides is searched, so that a theoretical basis is provided for controlling the viral disease of the micropterus salmoides and molecular marker assisted breeding work, a foundation is laid for cultivating new lines with strong disease resistance, and a scientific basis is provided for realizing the healthy and sustainable development of the aquaculture industry. The SNP markers which are obtained by screening and are related to the anti-infective performance of the largehead jewfish are used for screening parents with the anti-infective performance, so that the genetic breeding and breeding speed of the largehead jewfish is increased.
Description
Technical Field
The invention belongs to the technical field of fish genetic breeding, and particularly relates to an SNP marker related to antiviral properties of micropterus salmoides and application thereof.
Background
Pathogenic microorganisms causing diseases of micropterus salmoides mainly comprise fungi, bacteria, parasites and viruses, and the diseases are serious in viral diseases. Viral diseases are usually highly infectious and destructive, with varying latency, fast onset and high mortality. Pathogens of the largemouth bass viral diseases are mainly iridovirus and rhabdovirus. Among them, severe rhabdovirus disease of micropterus salmoides caused by rhabdovirus (Micropterus salmoides rhabdovirus, MSRV) can cause high mortality rate of micropterus salmoides fries, and cause huge economic losses to fries enterprises and farmers. Therefore, research on the resistance of the micropterus salmoides to the rhabdoviruses of the micropterus salmoides is particularly important. Up to now, no effective drugs or vaccines against the virus have been developed.
At present, various molecular marker assisted breeding technologies can be adopted in fish disease-resistant breeding, and two types of molecular markers such as microsatellite markers (simple sequence repeat, SSR) and single nucleotide molecular markers (single nucleotide polymorphism, SNP) are commonly used. Shao Yanqing, et al, selected an SSR marker associated with anti-lymphocystis disease virus (lymphocystis disease virus, LCDV) suitable for use in a chinese flounder population, which may be an SSR marker associated with anti-lymphocystis disease suitable for use in a chinese flounder population; by utilizing a molecular marker technology, SSR markers related to the infectious haematopoietic necrosis virus (Infectious haematopoietic necrosis virus, IHNV) of rainbow trout and SNP loci related to immune stress-resistant genes of the Miichthys miiuy (MIICHTHYS MIIUY) are screened, and a good foundation is laid for carrying out subsequent molecular marker-assisted breeding work.
Disclosure of Invention
The invention aims to provide an SNP marker related to antiviral property of micropterus salmoides and application thereof, thereby providing a screening marker of good breeding varieties for the micropterus salmoides and further providing an effective technical means for green and healthy fish breeding.
The invention firstly provides an SNP molecular marker related to rhabdovirus resistance of micropterus salmoides, wherein the SNP molecular marker is positioned at the 201 st position of the sequence of SEQ ID NO.1 and is replaced by T/C.
AATTTTAGACTGCTTCTGGTAGTCTGGCTTTGGCTTTAGGGTTTCAGAGCAGTTCTAGTTCTAGTTAAGGTTTAGTTCACATTTTGTTACTTCCAGTTTTGGTGTGTTGGAGTTGTGGTTCAAGCTAGGGTCTAGGGTTTTTTTTAATTACTTTTGGTGGTAGATTTGTCATAGTTTCCAAAGTGTTTTTCTGGTTTTAGTTGTGAGACTGAGACTGCCATTATGAGGCAACATTCATAGCATTCATCTGAAGTTGTGTCCCTGGCCACCTGGCTAATGTAAGTCCACTATATATCCACCAATATGCACTCTCCTTTTAGCTCTGTTTTTGGTCTCAACCAACTCCTGAGGGAAATATCTGCCCGGTAGCAGCTAAATTACTATTGTTAGGTTCATTATTA(SEQ ID NO:1);
The invention also provides a primer pair for detecting SNP loci, and the sequence information of the primers is as follows:
an upstream primer: 5'-GTTCTAGTTCTAGTTAAGG-3' (SEQ ID NO: 2);
a downstream primer: 5'-AGGGACACAACTTCAGATG-3' (SEQ ID NO: 3);
In another aspect, the present invention provides a method for screening anti-infective micropterus salmoides individuals, which is to screen individuals with specific genotypes of the SNP loci;
The individuals with the specific genotype are individuals with CC type;
the method is screened by a high-resolution melting curve (high resolution melting, HRM) method,
The method is completed by screening individual nucleic acid samples through PCR primer pairs, amplifying, sequencing amplified products and analyzing sequences.
According to the invention, the molecular marker is utilized to perform disease resistance correlation analysis, and a significant SNP locus related to the resistance to rhabdovirus infection of the micropterus salmoides is searched, so that a theoretical basis is provided for controlling the viral disease of the micropterus salmoides and molecular marker assisted breeding work, a foundation is laid for cultivating new lines with strong disease resistance, and a scientific basis is provided for realizing the healthy and sustainable development of the aquaculture industry. The SNP markers which are obtained by screening and are related to the anti-infective performance of the largehead jewfish are used for screening parents with the anti-infective performance, so that the genetic breeding and breeding speed of the largehead jewfish is increased.
Drawings
Fig. 1: gel electrophoresis result diagram;
Fig. 2: SNP locus typing result diagram.
Detailed Description
The obtained SNP locus related to the virus resistance of the micropterus salmoides can be used for screening parents infected by the virus resistance of the micropterus salmoides for breeding and predicting the infection character of progeny viruses so as to improve the efficiency of obtaining good varieties.
The SNP screened by the invention has the position of NW_024040041.1 chromosome 5852138 from the 5' end in the reference genome of the arca subcrenata, namely the 201 st position of the sequence of SEQ ID NO. 1, and the base is C or T.
The present invention will be described in detail with reference to the following examples and the accompanying drawings.
Example 1: screening antiviral individuals of Lateolabrax
1. Determination of semi-lethal concentration of MSRV virus on largemouth black bass
Randomly dividing 100 healthy largemouth black weever into 5 groups, diluting the suspension with the virus copy number of 3.05X10 5 copies/mL for 10 times to obtain 4 gradient diluents, respectively soaking 4 groups of experimental group fishes in a water body containing the viruses and the diluents, and adding oxygen during the period, wherein the volume is 5L, and adding water to 2/3 of the volume (50L) of a culture box after 30 min; the control group was immersed in a water body containing the same volume of PBS solution for the same period of time. The mortality was observed for 7d and calculated (no mortality during control) and the final half-lethal concentration of MSRV on micropterus salmoides (LC 50) was 3.05X10- 4 copies/mL (100 mL).
2. Anti-infective individual screening method
200 Healthy largemouth black bass is soaked in water containing largemouth black bass rhabdovirus suspension (concentration: 3.05X10 4 copies/mL), 30 early dead tails and 30 later surviving tails are collected and respectively used as a susceptibility group and a disease-resistant group, tail fins are cut off and stored in absolute ethyl alcohol for DNA extraction.
Example 2: screening for SNP loci associated with antiviral activity
1. Screening SNP loci capable of amplifying products
Based on transcriptome data results, on the basis of DEGs, the potential SNP sites were identified using GATK software identification. For the same site, 3 results of the experimental group are the same, 3 results of the control group are the same, and the same site, the experimental group and the control group are different, namely the point is considered to be changed into a high mutation rate, and the high mutation rate is listed in the primary screening result.
SNP primers were designed using PRIMERPREMIER software, requiring: the primer length is 18-25bp, the Tm value of the primer is 55-65 ℃, and the annealing temperature is about 60 ℃; the GC content of the primer is in the range of 40-70%; the target fragment has a length of 100-300bp and contains only 1 SNP site.
Table 1: information table of SNPs site primer candidates for micropterus salmoides part
2. Extraction of genome
Selecting anti-infection groups and easy-infection groups of micropterus salmoides fin bars, placing the anti-infection groups and easy-infection groups of micropterus salmoides fin bars into 1.5mL centrifuge tubes, adding 500 mu L of lysate and 15 mu L of proteinase K into each centrifuge tube with the fin bars, and performing water bath pyrolysis for 1 hour at 55 ℃ and shaking for a plurality of times to accelerate the pyrolysis. The lysed centrifuge tube was removed and 500 μl of phenol was added: chloroform: isoamyl alcohol is reversed and is gently shaken for 10min, and is centrifuged for 10min at 12000 r.p.m., 450 mu L of supernatant is taken and added into a centrifuge tube which is prepared in advance and is filled with 500 mu L of alcohol with corresponding number, and is gently shaken for 30 times, and is centrifuged for 5min at 12000 r.p., so that white DNA is precipitated at the bottom of the centrifuge tube. The liquid in the centrifuge tube was poured off, the DNA precipitate was dried in the air, 100. Mu. LddH 2 O was added, and the DNA was dissolved sufficiently by vortexing twice.
Amplification of DNA template
Randomly selecting 6 susceptible group and resistant group samples from the template samples, using the samples as templates for PCR amplification, amplifying by using an optimized PCR amplification system, and detecting the designed and synthesized primers.
Table 2: 20 mu L System Table for PCR reaction
The PCR products were detected by 2% agarose gel electrophoresis and primers with bright and single bands were selected (FIG. 1) for subsequent typing.
Genotyping was performed by HRM: the PCR amplification procedure is that the PCR amplification is performed for 10min at 95 ℃; denaturation at 95℃for 15s, annealing at 60℃for 15s, extension at 72℃for 10s for 45 cycles; finally, the mixture is preserved at the temperature of 4 ℃ after being extended at the temperature of 72 ℃ for 10min, and the melting curve conditions are as follows: the plate is read 15 times at the temperature of 60-95 ℃ and every 1 ℃.
Melting curve analysis was performed using the LightCycler96 software, the system is shown in Table 3 below.
Table 3: melting curve analysis system table
According to the typing result of SNPs locus, counting the genotype frequency and gene frequency, calculating the indexes of observing genetic diversity such as heterozygosity (Ho), expected heterozygosity (He), effective allele (Ne), hardy-Winberg equilibrium (HardyWeinberg equiliberum, HWE) and the like by using PopGene 32 and SPSS25 software, analyzing the correlation between SNP markers and disease resistance characters by using chi-square test, wherein P <0.05 indicates that the markers are obviously correlated with the characters.
The study uses SPSS25.0 to analyze genotype frequencies and allele frequencies of 35 SNP loci in an anti-susceptibility group and a susceptibility group, and uses chi-square test to conduct correlation analysis between the genotype frequencies and the allele frequencies and the disease resistance and susceptibility of MSRV. The results showed that there was a very significant difference distribution of SNP 13 gene frequencies in the anti-susceptibility and susceptibility groups (table 4) (P < 0.01).
Table 4: genotype and allele distribution frequency table of partial SNP loci in susceptible and disease resistant groups
The SNP13 locus is positioned on a cola a gene of collagen type I (type I, alpha 1 a) and positioned at the 201 st position of the sequence of SEQ ID NO.1, and is replaced by T/C.
AATTTTAGACTGCTTCTGGTAGTCTGGCTTTGGCTTTAGGGTTTCAGAGCAGTTCTAGTTCTAGTTAAGGTTTAGTTCACATTTTGTTACTTCCAGTTTTGGTGTGTTGGAGTTGTGGTTCAAGCTAGGGTCTAGGGTTTTTTTTAATTACTTTTGGTGGTAGATTTGTCATAGTTTCCAAAGTGTTTTTCTGGTTTTAGTTGTGAGACTGAGACTGCCATTATGAGGCAACATTCATAGCATTCATCTGAAGTTGTGTCCCTGGCCACCTGGCTAATGTAAGTCCACTATATATCCACCAATATGCACTCTCCTTTTAGCTCTGTTTTTGGTCTCAACCAACTCCTGAGGGAAATATCTGCCCGGTAGCAGCTAAATTACTATTGTTAGGTTCATTATTA(SEQ ID NO:1);
Primer pairs for detecting SNP loci, the sequence information of which is as follows:
an upstream primer: 5'-GTTCTAGTTCTAGTTAAGG-3' (SEQ ID NO: 2);
a downstream primer: 5'-AGGGACACAACTTCAGATG-3' (SEQ ID NO: 3);
SNP 13 locus is located at 5852138 of NW_024040041.1 chromosome col1a1a gene from 5' end, there are three genotypes of CC, TT and TC (figure 2), the genotype frequency and the gene frequency of the locus have extremely obvious difference distribution in anti-susceptibility group and susceptibility group, and the locus accords with Hardy-Weinberg balance condition. The result shows that the CC type of SNP 13 has a very significant correlation with disease resistance, and the TT type of SNP 13 has a very significant correlation with susceptibility.
Example 3: screening of anti-infective properties of micropterus salmoides using CC genotype of SNP13 locus
Healthy, well-developed, disease-free, injury-free and deformity-free largehead bass is selected, a fish fin sample is taken, genomic DNA is extracted, the site SNP13 marker is utilized to screen largehead bass individuals with the genotype of CC, the screened largehead bass is used as male and female parents for cultivation, and when the cultivation specification of the young fish reaches about 35g, anti-infection character test experiments are carried out. The result shows that the half lethal concentration (LC 50) of offspring of the Japanese perch with genotype of CC individuals is 5.3 multiplied by 10 4 copies/mL, which is obviously higher than that of the common group, and the offspring has stronger anti-infective property.
The results show that the micropterus salmoides screened by utilizing the SNP loci provided by the invention has the characteristic of resisting MSRV virus infection, and can be used for screening antiviral breeding parents.
Claims (9)
1. A SNP molecular marker is characterized in that the SNP molecular marker is positioned at the 201 st position of the sequence of SEQ ID NO.1 and is replaced by T/C.
2. A primer pair for detecting the SNP molecular marker as set forth in claim 1.
3. The primer pair of claim 2, wherein the sequence of the upstream primer is SEQ ID NO. 2 and the sequence of the downstream primer is SEQ ID NO. 3.
4. Use of a primer pair according to claim 2 for the preparation of a molecular preparation for detecting a SNP molecular marker according to claim 1.
5. The use of claim 4, wherein the preparation is a PCR amplification sequencing kit or a high resolution melting curve detection kit.
6. A method for screening individuals with anti-infective largemouth bass, which is characterized in that the method is to screen individuals with specific genotypes for detecting the SNP molecular markers as set forth in claim 1.
7. The method of claim 6, wherein the genotype-specific subject is a CC-type subject.
8. The method of claim 6, wherein the method is a high resolution melting curve method.
9. The method of claim 6, wherein the method is performed by amplifying a nucleic acid sample from a selected individual by PCR primer pairs, sequencing the amplified product, and analyzing the sequence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410311574.4A CN118127178A (en) | 2024-03-19 | 2024-03-19 | SNP marker related to antiviral property of micropterus salmoides and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410311574.4A CN118127178A (en) | 2024-03-19 | 2024-03-19 | SNP marker related to antiviral property of micropterus salmoides and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118127178A true CN118127178A (en) | 2024-06-04 |
Family
ID=91233976
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410311574.4A Pending CN118127178A (en) | 2024-03-19 | 2024-03-19 | SNP marker related to antiviral property of micropterus salmoides and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118127178A (en) |
-
2024
- 2024-03-19 CN CN202410311574.4A patent/CN118127178A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Transcriptome analysis of the Larimichthys crocea liver in response to Cryptocaryon irritans | |
| CN113957155B (en) | SNP molecular marker related to grass carp characters and application thereof | |
| Liu et al. | The complete mitogenome of Apocheima cinerarius (Lepidoptera: Geometridae: Ennominae) and comparison with that of other lepidopteran insects | |
| CN106811540B (en) | Microsatellite marker and specific primer for identifying male and female individuals of Pseudobagrus ussuriensis and application of microsatellite marker and specific primer | |
| CN108611429B (en) | Litopenaeus vannamei disease resistance related EST-SSR molecular marker and application thereof | |
| CN116121397A (en) | Reagent for detecting SNP molecular marker combination related to anti-nerve necrosis virus character of groupers | |
| CN110129456A (en) | A kind of anti-vibrios molecular labeling combination of prawn and its application in breeding | |
| KR101499695B1 (en) | Genetic markers and methods for identifying Epinephelus septemfasciatus and Epinephelus akaara among family Serranidae | |
| CN107988385B (en) | Method for detecting marker of PLAG1 gene Indel of beef cattle and special kit thereof | |
| CN107475414B (en) | Method for screening parent oysters with high glycogen content | |
| JP2023546627A (en) | Vibrio resistance-related EST-STR markers in vannamei shrimp, their specific primers, and detection methods | |
| KR101493978B1 (en) | Indel marker for discrimination of soybean cultivar | |
| CN104450697A (en) | SNP marker associated with oyster antiviral properties and application thereof | |
| CN110029174B (en) | SSR (simple sequence repeat) marker related to quality of macrobrachium rosenbergii bodies | |
| CN113373241B (en) | Microsatellite marker of fishes in loach, and amplification primer and application thereof | |
| CN118127178A (en) | SNP marker related to antiviral property of micropterus salmoides and application thereof | |
| CN112430675B (en) | Method for identifying anti-cysticercosis trait of bee colony by using SNP marker KZ 288474.1-322717 | |
| CN112210607B (en) | Molecular marker related to buffalo white hair phenotype and application thereof | |
| CN114959056A (en) | SSR marker for identifying female procambarus clarkii and application thereof | |
| CN114875157A (en) | SNP (Single nucleotide polymorphism) marker related to individual growth traits of pelteobagrus fulvidraco and application | |
| CN110951892B (en) | SSR primer pair group for identifying several sturgeon species, kit, identification method and application | |
| CN113322328A (en) | SNP molecular marker with cryptocaryon irritans disease resistance-related traits for trachinotus ovatus and application of SNP molecular marker | |
| CN110016510B (en) | Molecular marker for genetic breeding of macrobrachium rosenbergii | |
| CN112410441A (en) | Method for identifying anti-cysticercosis trait of bee colony by using SNP marker KZ 288479.1-95621 | |
| CN107653323B (en) | Megalobrama amblycephala transferrin gene SNP molecular marker and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |