CN118045220A - Cur-PF127/SA-CS/PVA hydrogel composite material and preparation method and application thereof - Google Patents

Abstract

The invention provides a Cur-PF127/SA-CS/PVA hydrogel composite material and a preparation method thereof, wherein the hydrogel composite material is prepared from borax, sialylated chitosan (SA-CS), polyvinyl alcohol (PVA) and curcumin-loaded Pluronic F127 micelle through forming boric acid ester bonds between PVA, SA-CS and borax under physiological conditions, and the prepared Cur-PF127/SA-CS/PVA hydrogel composite material has excellent mechanical property, gel dynamics and antibacterial property. Experiments show that the hydrogel composite material shows good biocompatibility when incubated with NIH3T3 cells, which shows that the hydrogel composite material has great potential application value in the aspect of developing clinical materials for wound healing.

Description

Cur-PF127/SA-CS/PVA hydrogel composite material and preparation method and application thereof

Technical Field

The invention belongs to the technical field of hydrogel material preparation, and particularly relates to a Cur-PF127/SA-CS/PVA hydrogel composite material, and a preparation method and application thereof.

Background

It is well known that wound wounds cause skin damage, which can increase endogenous bacterial infection and affect human health. Wound healing is a complex process involving hemostasis, inflammation, proliferation and remodeling steps. Some wounds heal, such as diabetic wounds, may take longer to repair, increasing the risk of further infection and exacerbating the possibility of more severe (i.e., necrosis) or even life threatening. Therefore, it is important to synthesize a functional wound dressing that not only promotes wound healing, but also requires better antimicrobial properties. Various biomaterials have been developed, such as foams, films, nanoplatelets, and hydrogels, with injectable hydrogels being considered most promising. This is due to its tunable superior antimicrobial and self-healing properties, which will help to increase the overall efficiency of wound healing.

Thus, the preparation of the functional hydrogel can well prevent wound infection and accelerate wound healing. Hydrogels derived from natural polysaccharide-based biomaterials have received great attention in order to solve this problem. This is due to their excellent biocompatibility, making them a promising candidate for numerous applications in the biomedical industry. Among these, chitosan (CS) is a nontoxic, biodegradable and biocompatible aminopolysaccharide produced from chitin present in the outer bones (shells) of arthropods and the cell walls of fungi. As one of the most abundant natural materials, it is also widely used as a biomaterial due to its biocompatibility and antibacterial properties. However, its poor solubility in solvents (i.e., polar or nonpolar) limits the versatility of application. Thus, chitosan generally requires chemical modification with different functional groups by reaction with primary and secondary hydroxyl groups on the chitosan backbone to enhance its biological activity.

Curcumin (Cur), also known as isopropyl methane, is present in turmeric herbs and has good biological properties such as anti-inflammatory, hypoglycemic, wound healing properties, etc., making it potentially beneficial to human health. However, cur has the defects of poor solubility in water, poor bioavailability, poor light stability and the like, and related documents for preparing hydrogel by combining curcumin and chitosan are not recorded at present, so how to combine the curcumin and the chitosan to prepare a novel self-repairing hydrogel composite material is a main technical problem to be solved by the invention.

Disclosure of Invention

The invention aims to provide a Cur-PF127/SA-CS/PVA hydrogel composite material, and simultaneously provides a preparation method and application thereof, which are the second invention aim, and the hydrogel composite material prepared by the invention has excellent self-repairing and antibacterial properties, can be used for promoting wound healing and treatment, and also has the risk of preventing wound infection.

In order to achieve the aim of the invention, the invention adopts the following technical scheme:

a Cur-PF127/SA-CS/PVA hydrogel composite material is prepared from borax, sialylated chitosan SA-CS, PVA and curcumin-loaded Pluronic F127 micelle.

The invention also discloses a preparation method of the Cur-PF127/SA-CS/PVA hydrogel composite material, which comprises the steps shown in figure 1.

As a further preferred aspect of the present invention, in the above preparation method, the specific preparation process of each step is:

(a) Synthesis of sialyl chitin SA-CS: dissolving 0.8-1.2g sialic acid, 0.3-0.5g N-hydroxysuccinimide (NHS) and 0.5-0.8g 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) in 0.1M 2-morpholinoethanesulfonic acid (MES buffer solution) with pH of 5.5, stirring for 0.25-0.75h, adding into chitosan-acetic acid solution, slowly stirring at room temperature for reacting for 10-14h, dialyzing, and freeze-drying to obtain SA-CS;

(b) Preparation of curcumin micelle Cur-PF 127: according to the previous experiments, curcumin and Pluronic127 were first mixed at 1:99 Adding the material proportion into dichloromethane according to the mass ratio, stirring to dissolve the material proportion and the dichloromethane, performing rotary evaporation in a rotary evaporator at 38-45 ℃, adding deionized water into the obtained thick liquid, continuously stirring to obtain self-assembled curcumin micelle Cur-PF127, and freeze-drying to obtain Cur-PF127 yellow powder;

(c) Synthesis of Cur-PF127/SA-CS/PVA hydrogel: adding SA-CS and PVA in the step (a) into 5ml PBS with pH of 7.2 respectively, and dissolving at 37 ℃ to obtain mixed solution I of SA-CS and PVA; adding the Cur-PF127 in the step (b) into the mixed solution I to obtain a mixed solution II, and adding 900 mu L of 4wt% borax into the mixed solution II to mix so as to obtain the Cur-PF127/SA-CS/PVA composite hydrogel.

As a further preferred aspect of the present invention, in the step (a), the chitosan-acetic acid solution is obtained by dissolving 1.5-2.0g of chitosan in 220ml of 1% acetic acid aqueous solution, and adding 5M NaOH to adjust pH to 5.5-6.5; during dialysis, a 6000-8000Da dialysis bag is adopted to dialyze in distilled water for 70-74 hours.

As a further preferred aspect of the present invention, in the step (c), the content of SA-CS and PVA in the mixed liquor I is 3.5% w/v; the content of Cur-PF127 in the mixed solution II is 1-5% (w/v), preferably 1-3% (w/v).

As a further preference of the invention, the invention further discloses the application of the Cur-PF127/SA-CS/PVA hydrogel composite material in wound dressing, wherein the freeze-drying temperature is-50 ℃ in the step (a) and the step (b).

Compared with the prior art, the invention has the following beneficial effects:

The curcumin is subjected to micelle formation to enhance the delivery of the curcumin in the water-mediated hydrogel so as to promote the improvement of the bioavailability of the Cur, and the obtained Cur-PF127/SA-CS/PVA hydrogel composite material has excellent mechanical property, gel dynamics and antibacterial property. When co-incubated with NIH3T3 cells, the hydrogel composite material exhibits good biocompatibility, which indicates that the hydrogel composite material of the invention also has great potential application value in developing clinical materials for wound healing.

Drawings

FIG. 1 is a schematic diagram of a synthetic process of Cur-PF127/SA-CS/PVA hydrogels of the present invention;

FIG. 2 is a graph showing the characterization of SA-CS and Cur-PF127 micelles; wherein, (a) ¹ H NMR spectra of CS and SA-CS; (b) A comparison plot of free curcumin in water (left side) and Cur-PF127 micelles (right side); (c, d) are TEMs of different magnifications of the Cur-PF127 micelles, and (e) size distribution of the Cur-PF127 micelles calculated according to the TEMs;

FIG. 3 (a) is a photograph showing Gel-3 as an example, which is obtained by confirming the formation of a hydrogel by inversion; (b) a physical photograph showing Gel-3 with self-repair; (c) is an SEM image of Gel 1-Gel 4; (d) is a swelling property test chart of Gel 1 to Gel 3;

FIG. 4 is a graph showing rheological property tests of the prepared hydrogels Gel1 to Gel 4. (a) (b) is a plot of storage modulus (G ') and loss modulus (G') over time in a time sweep in the rheological properties of Gel1-Gel 4; (b) (d) is a frequency sweep pattern in rheological properties in which storage modulus (G') and loss modulus (G ") are plotted as a function of frequency;

FIG. 5 (a) is the ability of Gel1-Gel4 to scavenge ABTS free radicals; (b) And (c) is the inhibitory capacity of Gel1-Gel4 against E.coli and Staphylococcus aureus;

FIG. 6 shows cell surface mobility experiments on Gel1-Gel 4;

FIG. 7 (a) is a NIH-3T3 cell compatibility experiment for Gel1-Gel4 gels; (b) Cell fluorescence patterns after 1 day, 3 days and 5 days of co-culture of Gel1-Gel4 extract and NIH-3T3 cells respectively.

Detailed Description

In order to make the contents of the present invention more easily understood, the technical scheme of the present invention will be further described with reference to the specific embodiments, but the present invention is not limited thereto. The room temperature in the invention is 20-30 ℃, and the effect of the invention can be realized as long as the room temperature condition is satisfied.

Example 1

A novel self-repairing hydrogel composite material based on chitosan and curcumin is prepared from borax, sialylated chitosan, PVA and curcumin loaded Pluronic F127 micelle.

The preparation method, as shown in figure 1, comprises the following steps:

(a) Synthesis of sialylacetylpolysaccharide SA-CS (see FIG. 1 a): 1.8g of chitosan is dissolved in 220ml of 1% acetic acid aqueous solution, and 5M NaOH is added to adjust the pH to 6 to obtain chitosan-acetic acid solution; 1.0g sialic acid, 0.45g N-hydroxysuccinimide (NHS) and 0.74g 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) are dissolved in 0.1M 2-morpholinoethanesulfonic acid (MES buffer solution) with pH of 5.5, stirred for 0.5h, added into chitosan-acetic acid solution and slowly stirred at room temperature for reaction for 12h; after the reaction is finished, the preparation method comprises the steps of dialyzing and freeze-drying at minus 50 ℃; dialyzing with 7000Da dialysis bag in distilled water for 72 hr;

(b) Preparation of curcumin micelle Cur-PF127 (see FIG. 1 b): curcumin and Pluronic 127 polymer were first blended at 1:99, slowly stirring and dissolving in dichloromethane, spin-evaporating in a rotary evaporator at 40 ℃, adding the obtained thick liquid into deionized water, continuously stirring and self-assembling to obtain curcumin micelle Cur-PF127, and freeze-drying at-50 ℃ to obtain Cur-PF127 yellow powder;

(c) Synthesis of Cur-PF127/SA-CS/PVA hydrogel (see FIG. 1 c): adding SA-CS and PVA obtained in the step (a) into 5ml PBS with pH of 7.2 respectively, and dissolving at 37 ℃ to obtain mixed solution I of SA-CS and PVA; adding the Cur-PF127 powder obtained in the step (b) into the mixed solution I to obtain a mixed solution II, and adding 900 mu L of 4wt% borax into the mixed solution II to mix so as to obtain Cur-PF127/SA-CS/PVA composite hydrogel; in the step (c), the content of SA-CS and PVA in the mixed solution I is 3.5% w/v; in the mixed solution II, the content of Cur-PF127 is 3% (w/v).

Example 2

The Cur-PF127/SA-CS/PVA hydrogel composite material and the preparation method thereof in the embodiment are different from those in the embodiment 1 in that:

In step (a), synthesis of sialyl chitosan SA-CS: 1.5g of chitosan is dissolved in 220ml of 1% acetic acid aqueous solution, and 5M NaOH is added to adjust the pH to 5.5 to obtain chitosan-acetic acid solution; dissolving 0.8g sialic acid, 0.3g N-hydroxysuccinimide (NHS) and 0.5g 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) in 0.1M 2-morpholinoethanesulfonic acid (MES buffer solution) with pH of 5.5, stirring for 0.25h, adding into chitosan-acetic acid solution, slowly stirring at room temperature for reacting for 10h, dialyzing, and freeze-drying at-50 ℃ to obtain SA-CS; dialyzing in distilled water with 6000Da dialysis bag for 70 hr;

In the step (b), curcumin micelle Cur-PF127 is prepared: curcumin and Pluronic 127 were first combined at 1:99 is added into dichloromethane according to the mass ratio of the materials, and the dichloromethane and the mixture are stirred to be dissolved, then the mixture is distilled in a rotary evaporator at 38 ℃, deionized water is added into the obtained thick liquid, the mixture is continuously stirred to obtain self-assembled curcumin micelle Cur-PF127, and the self-assembled curcumin micelle Cur-PF127 yellow powder is obtained by freeze-drying at-50 ℃.

Example 3

The Cur-PF127/SA-CS/PVA hydrogel composite material and the preparation method thereof in the embodiment are different from those in the embodiment 1 in that:

In step (a), synthesis of sialyl chitosan SA-CS: 2.0g of chitosan is dissolved in 220ml of 1% acetic acid aqueous solution, and 5M NaOH is added to adjust the pH to 6.5 to obtain chitosan-acetic acid solution; dissolving 1.2g of sialic acid, 0.5g of N-hydroxysuccinimide (NHS) and 0.8g of 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) in 0.1M 2-morpholinoethanesulfonic acid (MES buffer solution) with pH of 5.5, stirring for 0.75h, adding into chitosan-acetic acid solution, slowly stirring at room temperature for reaction for 14h, dialyzing and freeze-drying at-50 ℃ to obtain SA-CS; dialyzing with 8000Da dialysis bag in distilled water for 74 hr;

In the step (b), curcumin micelle Cur-PF127 is prepared: curcumin and Pluronic 127 were first combined at 1:99 is added into dichloromethane according to the mass ratio of the materials, and the dichloromethane and the mixture are stirred simultaneously to dissolve the two materials, then the mixture is steamed in a rotary evaporator at 45 ℃, the obtained thick liquid is added into deionized water, the mixture is continuously stirred to obtain self-assembled curcumin micelle Cur-PF127, and the self-assembled curcumin micelle Cur-PF127 yellow powder is obtained by freeze-drying at-50 ℃;

Performance test:

In the performance test, the related testing methods of hydrogel morphology test, antioxidant activity test, rheology evaluation, swelling experiment, antibacterial experiment and the like are as follows:

1. Hydrogel morphology determination:

After initial freezing in liquid nitrogen and drying for 12 hours, gold was used to coat the hydrogel. The gold-plated samples were then analyzed by scanning electron microscopy (SEM; hitachi, S-4800).

2. Test of antioxidant Activity

The 7mM ABTS solution is added with 2.5mM potassium persulfate and mixed and oxidized for 12-16 hours at room temperature under the dark condition to obtain ABTS+. The antioxidant activity was then measured using an ABTS+ solution, and specifically, 0.05g of the sample to be tested was added to 5mL of the ABTS+ solution while ensuring that the absorbance of the solution at 734nm was 0.700.+ -. 0.020. The mixture was then shaken and incubated in the dark at 37℃for 30min. Finally, the absorbance of the reaction solution was measured at 734 nm. The free radical scavenging efficiency was calculated as follows:

wherein [ A ] Blank represents the absorption of the Blank, and [ A ] Sample represents the absorption of the Sample. The blank here refers only to ABTS solution, and the sample solution refers to abts+ sample solution.

3. Rheology evaluation

The composite hydrogels were rheometrically measured using a rheometer (TA, DHR, USA). The rheometer was equipped with a 20mm stainless steel upper cone and temperature controlled Peltier plate (DISCOVERYHR-2, TA, USA) at 37 ℃. 400 μl aliquots of SA-CS, cur-PF127 and PVA solution were introduced into Peltier, after which the cone was lowered to the indicated gap. Water evaporation was prevented by placing low viscosity oil, after which gelation was started using 70 μl borax (4 wt%) and the borax was injected into the gap. To determine the storage modulus and gelation kinetics, a dynamic time sweep of 1Hz was performed at 1% applied strain for a duration of 2000s. The elastic behavior of the solution (now hydrogel) after gelation is determined by dynamic frequency sweep at 1% strain of 0.1-100rad/s and strain sweep at 1Hz of 0.1% -500% (or failure).

4. Swelling test

The lyophilized hydrogel was introduced into PBS (2 mL) at room temperature and maintained for a specified time interval. At various time intervals, the hydrogel was recovered and cleaned using filter paper to remove additional surface fluid, and the Swelling Ratio (SR) was calculated as follows:

Wherein W ₀ and Wt represent the initial mass (g) of the hydrogel after freeze-drying and the final mass (g) of the hydrogel after swelling, respectively.

5. Antibacterial property test

Antibacterial activity of hydrogels was evaluated using gram positive and gram negative microorganisms of staphylococcus aureus (s.aureus) and escherichia coli (e.coli), respectively. The hydrogel was introduced into Luria Bertani (LB) medium (0.02 g/10 mL) and then diluted bacterial suspension (10. Mu.L) was added. The mixture was then incubated at 37℃for 12 hours. The mixture was then measured for O.D 60 at 600 nm. For consistency, a blank containing all the above components without hydrogel was used as a control group, so that the Antibacterial Rate (AR) was calculated by the following equation:

Where Kb represents the absorption of the blank, and Ks represents the absorption of the hydrogel-containing sample.

6 Test results:

6.1 structural characterization and Performance test of SA-CS and Cur-PF127 micelles

FIG. 2 is a graph showing the characterization of SA-CS and Cur-PF127 micelles; wherein, (a) 1HNMR spectra of CS and SA-CS; (b) A comparison plot of free curcumin in water (left side) and Cur-PF127 micelles (right side); (c, d) are TEMs of different magnifications of the Cur-PF127 micelle, and (e) are size distributions of the Cur-PF127 micelle calculated according to the TEM.

From the hydrogen nuclear magnetic resonance analysis of chitosan and modified SA-CS before unmodified as shown in FIG. 2a, the results indicate that SA was successfully modified to the CS backbone; FIG. 2b is a comparison of the free state (left side) and Cur-PF127 micelles (right side) in pure curcumin water, with macroscopic unmodified curcumin having poor solubility, whereas Cur-PF127 showed good dissolution effect, which can reach 3mg/mL. As can be seen from FIGS. 2d and 2e, the morphology of Cur-PF127 is spherical with a diameter of _～ 33.5.5.+ -. 4.2nm.

6.2 Physicochemical Properties of hydrogels

The present invention adds borax solution to PVA/SA-CS/Cur-PF127 solution to form hydrogel, and confirms the formation of hydrogel by tube inversion method, as shown in FIG. 3 a.

To investigate the effect of the Cur-PF127 content on the properties of the hydrogels, 0%,1%,3% and 5% (w/v) of the hydrogels were obtained by substituting Cur-PF127 content of 0.000g,0.005g,0.015g and 0.020g, respectively, based on example 1. The hydrogels obtained were Gel 1, gel 2, gel 3 and Gel 4, respectively, example 1 was designated as Gel 3, and Cur-PF127 content was 3% (w/v).

The morphology of the freeze-dried composites was evaluated using SEM for the resulting Gel1-Gel 4 and their porosity and interconnecting properties were determined as shown in FIG. 3 b. As can be seen from fig. 3b, the hydrogel of the present invention has a highly porous structure, which is advantageous for promoting cell migration, while the pore size and porosity of the composite hydrogel decrease as the Cur-PF127 micelle is introduced to increase. The three-dimensional (3D) structure of the lyophilized hydrogel has an interconnected pore structure, which is conducive to migration and growth of wound edges in the hydrogel, provides conditions for rapid healing of different wound tissues, and the prepared composite hydrogel exhibits a better reversible construction of self-healing dynamic covalent bonds, and in order to further illustrate the self-healing properties of the present invention, gel-3 (inventive example 1) is cut and separated, one half of which is stained with methylene blue and the other half of which is not stained, and the separated two parts remain intact for a period of time upon contact, which indicates that the hydrogels of the present invention have good self-healing properties; FIG. 3d shows the results of swelling property tests of Gel1-Gel 3, and FIG. 3 shows that all hydrogels reach an equilibrium swelling state within one hour, where it should be noted that the swelling data without Gel 4 is caused by hydrogel collapse swelling failure during the swelling experiment, and that Gel 2 and Gel3 show good swelling properties compared with Gel1, and that Gel1-Gel 3 have swelling rates of 370.+ -. 5%, 550.+ -. 10% and 600.+ -. 9%, respectively. Therefore, the content of Cur-PF127 is selected to be 1% -3% in the invention.

6.3 Rheological Properties of hydrogels

FIG. 4 is a graph showing rheological property tests of the prepared hydrogels Gel1 to Gel 4. In the time scan test, in general, both the storage modulus (G') (a) and the loss modulus (G ") (b) of the gel are increased with time, the storage modulus is always higher than the loss modulus, and the structure of the surface gel has good stability. Of the four gels (Gel 1 to Gel 4) prepared, the storage modulus of the Gel decreased as the curcumin content increased, as shown in (a). The storage modulus (c) and loss modulus (d) of the gel also remained relatively stable over the frequency range of 0.1-10Hz during the frequency sweep, which also indicated that the hydrogels prepared had good structural stability. In the stress amplitude sweep, the storage modulus (e) and the loss modulus (f) of Gel1 to Gel4 can be kept stable when the stress is small, but decrease as the stress increases, the storage modulus and the loss modulus start to decrease, and the Gel structure is destroyed. (g) The viscosity of Gel 3 is plotted against shear force, which shows that the Gel has shear thinning properties and thus injectability. (h) Is a self-healing rheological schematic of Gel 3 (inventive example 1).

6.4 Antioxidant and antibacterial Properties of hydrogels

FIG. 5 (a) shows the oxidation resistance of the prepared gels Gel 1 to Gel 4, and it can be seen from FIG. 5 that the oxidation resistance of the Gel is stronger as the curcumin content is increased. Curcumin is used as a natural flavonoid compound, and has not only an antioxidant function, but also antibacterial performance. The curcumin-coated gel can inhibit not only staphylococcus aureus, but also escherichia coli (c). In general, the prepared hydrogel has better inhibitory effect on staphylococcus aureus than on escherichia coli.

6.5 Migration experiments of cells on hydrogel surface

FIG. 6 is a migration experiment of cells on the surface of hydrogel. The prepared hydrogel is composed of natural polysaccharide, so that the hydrogel has good compatibility. As can be seen from fig. 6, an increase in the curcumin content can accelerate the migration effect of cells.

6.6 Compatibility test of cells

FIG. 7 is a cell compatibility experiment. The cell compatibility test is a necessary test means for evaluating the application of biological materials in animal experiments. As can be seen from FIG. 7 (a), the number of NIH-3T3 cells increased significantly over time, indicating good compatibility of the material. In addition, after a period of co-culture of the aqueous extract of the gel with the cells, the number of cells also tends to increase as seen under the light microscope (b). The two experiments show that the material has low toxicity, good cell compatibility and great potential for being applied to skin wound repair materials in clinic.

The foregoing description is only of the preferred embodiments of the invention, and all changes and modifications that come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims (7)

1. The Cur-PF127/SA-CS/PVA hydrogel composite material is characterized by being prepared from borax, sialylated chitosan SA-CS, PVA and curcumin-loaded Pluronic F127 micelle.

2. The method for preparing the Cur-PF127/SA-CS/PVA hydrogel composite material according to claim 1, wherein the method comprises the steps shown in FIG. 1.

3. The method for preparing the Cur-PF127/SA-CS/PVA hydrogel composite material according to claim 2, wherein the specific preparation process of each step is as follows:

(a) Synthesis of sialyl chitin SA-CS: dissolving 0.8-1.2g sialic acid, 0.3-0.5g N-hydroxysuccinimide (NHS) and 0.5-0.8g 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) in 0.1M 2-morpholinoethanesulfonic acid (MES buffer solution) with pH of 5.5, stirring for 0.25-0.75h, adding into chitosan-acetic acid solution, stirring at room temperature for reacting for 10-14h, dialyzing, and freeze-drying to obtain SA-CS;

(b) Preparation of curcumin micelle Cur-PF 127: curcumin and Pluronic 127 were first combined at 1:99 Adding the material proportion into dichloromethane according to the mass ratio, stirring to dissolve the material proportion and the dichloromethane, performing rotary evaporation at 38-45 ℃, adding deionized water into the obtained thick liquid, continuously stirring to obtain self-assembled curcumin micelle Cur-PF127, and freeze-drying to obtain Cur-PF127 yellow powder;

(c) Synthesis of Cur-PF127/SA-CS/PVA hydrogel: adding the SA-CS and PVA in the step (a) into 5ml PBS with pH7.2, and dissolving at 37 ℃ to obtain a mixed solution I of the SA-CS and the PVA; adding the Cur-PF127 in the step (b) into the mixed solution I, mixing to obtain a mixed solution II, and adding 900 mu L of 4wt% borax into the mixed solution II, mixing to obtain the Cur-PF127/SA-CS/PVA composite hydrogel.

4. The method of preparing a Cur-PF127/SA-CS/PVA hydrogel composite according to claim 3, wherein in the step (a), the chitosan-acetic acid solution is obtained by dissolving 1.5-2.0g of chitosan in 220ml of 1% acetic acid aqueous solution, and adding 5M NaOH to adjust the pH to 5.5-6.5; during dialysis, a 6000-8000Da dialysis bag is adopted to dialyze in distilled water for 70-74 hours.

5. The method of preparing a Cur-PF127/SA-CS/PVA hydrogel composite material according to claim 3, wherein in step (c), the contents of SA-CS and PVA in the mixed solution I are 3.5% w/v; in the mixed solution II, the content of Cur-PF127 is 1-3% (w/v).

6. The method of preparing a Cur-PF127/SA-CS/PVA hydrogel composite material according to claim 3, wherein in step (a) and step (b), the lyophilization temperature is-50 ℃.

7. Use of the Cur-PF127/SA-CS/PVA hydrogel composite material according to claim 1 in a wound dressing.