CN118006469A - Mucor pulmonale capable of degrading polyurethane plastic and application thereof - Google Patents
Mucor pulmonale capable of degrading polyurethane plastic and application thereof Download PDFInfo
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- 239000004814 polyurethane Substances 0.000 title claims abstract description 48
- 229920003023 plastic Polymers 0.000 title claims abstract description 43
- 239000004033 plastic Substances 0.000 title claims abstract description 43
- 229920002635 polyurethane Polymers 0.000 title claims abstract description 29
- 230000000593 degrading effect Effects 0.000 title claims description 10
- 241000235395 Mucor Species 0.000 title description 3
- 230000015556 catabolic process Effects 0.000 claims abstract description 29
- 238000006731 degradation reaction Methods 0.000 claims abstract description 29
- 229920001896 polybutyrate Polymers 0.000 claims abstract description 16
- 229920005830 Polyurethane Foam Polymers 0.000 claims abstract description 8
- 239000011496 polyurethane foam Substances 0.000 claims abstract description 8
- 241001529553 Scoparia <angiosperm> Species 0.000 claims abstract 6
- 230000001580 bacterial effect Effects 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 241001207476 Sarocladium sp. Species 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 5
- 241000222290 Cladosporium Species 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 2
- 239000002362 mulch Substances 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 239000003124 biologic agent Substances 0.000 claims 2
- 240000006394 Sorghum bicolor Species 0.000 claims 1
- 235000015505 Sorghum bicolor subsp. bicolor Nutrition 0.000 claims 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims 1
- 230000002906 microbiologic effect Effects 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract description 5
- 241000395107 Cladosporium cucumerinum Species 0.000 abstract description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052799 carbon Inorganic materials 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 238000006065 biodegradation reaction Methods 0.000 description 7
- 239000006260 foam Substances 0.000 description 6
- 108020004463 18S ribosomal RNA Proteins 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000004064 recycling Methods 0.000 description 5
- 230000004580 weight loss Effects 0.000 description 5
- 239000002689 soil Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000013585 weight reducing agent Substances 0.000 description 3
- 241000228197 Aspergillus flavus Species 0.000 description 2
- 241000800293 Sarocladium Species 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 229920001228 polyisocyanate Polymers 0.000 description 2
- 239000005056 polyisocyanate Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 150000003077 polyols Chemical class 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000458359 Brevibacillus sp. Species 0.000 description 1
- 241001478778 Cladophora Species 0.000 description 1
- 241001207508 Cladosporium sp. Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241001397809 Hakea leucoptera Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241000239226 Scorpiones Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
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- 239000008272 agar Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000002856 computational phylogenetic analysis Methods 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- -1 food-grade coatings Substances 0.000 description 1
- 238000004362 fungal culture Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000012212 insulator Substances 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000013502 plastic waste Substances 0.000 description 1
- 239000004014 plasticizer Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 238000010248 power generation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- 239000003381 stabilizer Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/60—Biochemical treatment, e.g. by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B2101/00—Type of solid waste
- B09B2101/75—Plastic waste
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B2101/00—Type of solid waste
- B09B2101/75—Plastic waste
- B09B2101/78—Plastic waste containing foamed plastics, e.g. polystyrol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
Abstract
The invention discloses a strain of cladosporium cucumerinum and application thereof in degradation of polyurethane plastics. A strain of degradable polyurethane foam plastic, the storage number of which is GDMCC No, of the scoparia viridis SC 23: 63578. the scoparia SC23 can grow by taking IMPRANIL DLN as a unique carbon source, and has wide environmental adaptability and plastic degradation potential. The degradation efficiency of the strain on 6g/L polyurethane foam reaches 94.17% within 20 days, and the degradation efficiency on 1g/L agricultural mulching film PBAT reaches 37.86% within 30 days. The volume of the plastic is obviously reduced after the plastic is degraded by the strain, and the surface of the plastic is eroded. Therefore, the invention provides important biological resources for the biological treatment of waste polyurethane plastics and agricultural mulching films.
Description
Technical Field
The invention belongs to the field of applied microbiology, and relates to a strain of Cladosporium virens and application thereof in degrading polyurethane plastics and agricultural mulching film PBAT.
Background
The plastic is a product which is formed by plasticizing an organic high molecular polymer serving as a main component and a plasticizer, a stabilizer or an additive serving as an auxiliary element according to a basic chemical method. The use of plastics was widely mined by humans in the early 20 th century, has now become a major commodity worldwide, has grown exponentially in modern society, and has penetrated almost every aspect of human life. Polyurethane (PU) is a polymer polymerized from a polyisocyanate and a polyol, wherein the polyisocyanate forms the hard segment of the polyurethane, imparting hardness and rigidity to the polyurethane material; the polyol forms the soft segment of the polyurethane and imparts elasticity to the polyurethane material. Polyurethane (PU) has wide application in household, industrial and medical fields, and its special structure makes it have high tensile strength, high hydrolysis resistance, high melting point and other characteristics. For adhesives, foams, food-grade coatings, insulators, tires, sponges and more products. The global polyurethane yield of 2016 was about 2230 ten thousand tons and the annual growth rate was 4.0%, and in europe alone, polyurethane plastics account for approximately 7.7% of the total plastic demand. In australia, about 250 ten thousand tons of plastic waste are produced in 2016-2017, of which only 12% is recycled and 87% is dumped in landfills. The waste plastic is discarded to cause serious ecological pollution in the environment and is also a waste of carbon resources.
At present, the recycling of waste plastics mainly adopts modes of classified recycling, physical and chemical treatment degradation and utilization, incineration power generation and the like. In recent years, the biodegradation of plastics and the recycling of degradation products by means of microorganisms have become alternative means for recycling plastics. In recent years, with importance attached to plastic biodegradation at home and abroad, a plurality of polyurethane plastic degrading microorganisms have been reported, including fungi such as aspergillus flavus a.flavus G10 (CN 109762744 a), aspergillus Aspergillus flavus (202010879237.7), cladosporium Cladosporium sp.p7 (20201412031. X), saccharomycetes (CN 202210403010.4) and bacteria such as bacillus Brevibacillus sp.p10 (202011442856.6). However, the problems of low degradation efficiency, few degradation biological resources, lack of degradation technology and the like are limited, and the polyurethane plastic biodegradation is still in the resource accumulation and laboratory research stage at present, so that the high-efficiency degradation microbial resources are less. Therefore, the comprehensive multiple evaluation means further excavate the high-efficiency polyurethane degrading microorganism, which has important significance for realizing the biodegradation and the recycling of the polyurethane plastics.
Disclosure of Invention
The invention provides a cladosporium cucumerinum Sarocladium sp.SC23 and application thereof in biodegradation of polyurethane plastics.
Fungus SC23 of degradable polyurethane foam, classified and named as Cladosporium virginum (Sarocladium sp.) is deposited in the microorganism strain collection center of Guangdong province, the deposit address is the institute of microorganisms of the university of No. 59 building No. 5 building, guangdong province, guangzhou City martyr, the deposit date is 2023, month 6, and 21, and the deposit number is GDMCC No:63578.
The invention discloses a method for preparing a pure culture of a bacterial strain, which is characterized in that the Scorpion SC23 is prepared by taking collected soil of needle forest suitable for spring as a material, adding 2g/L IMPRANIL DLN of an inorganic salt culture medium for culture, judging whether the culture has a polyurethane degradation function through hydrolysis of a transparent ring, and separating and purifying the culture. SC233 was initially identified as Mucor pulmonale by strain 18S rRNA sequence alignment analysis, the 18S rRNA sequence being shown in SEQ ID NO. 1.
The culture, bacterial strain liquid, bacterial strain fermentation culture liquid or filtrate of fermentation culture liquid of the cladosporium cucumerinum SC 23.
The invention relates to a biological microbial inoculum taking the cladophora scoporia SC23 as an active ingredient.
The invention relates to a method for preparing a scoporia vaginalis SC23 and application of a culture, a bacterial strain liquid, a bacterial strain fermentation culture liquid or a filtrate of the fermentation culture liquid in plastic biodegradation.
As a preference of the invention, the plastic is selected from IMPRANIL DLN, PBA-PU, real polyurethane PUR plastic, agricultural mulching film PBAT and the like.
As a further preferred aspect of the invention, the polyurethane PUR foam with a concentration of 6g/L is inoculated with a spore suspension with a spore concentration of 1X 10 6 at 2% and a weight loss rate of 94.17% within 20 days at 30℃and 180rpm under degradation conditions of inorganic salt medium; the weight loss rate of the agricultural mulching film PBAT with the concentration of 1g/L is 37.86 percent within 30 days
Advantageous effects
The invention adopts a solid culture medium containing 2g/L IMPRANIL DLN inorganic salt to successfully screen a strain SC23 with polyurethane degradation capability from the soil of needle woods in northeast China, and the 18S rRNA sequence of the strain is aligned in NCBI, so that the result shows that the homology with the Scopulariella (Sarocladium sp.) is highest, and therefore, the strain is identified as Sarocladium sp.SC23. The strain adopted by the invention can degrade the waterborne polyurethane IMPRANIL DLN and the PBA-PU in the growth process, and a transparent ring is produced on a 2g/L IMPRANIL DLN or 1g/L PBA-PU inorganic salt solid culture dish. In addition, the strain has higher degradation effect on polyurethane PUR foam and agricultural mulching film PBAT. Weight reduction experiments based on polyurethane PUR foam and agricultural mulching film PBAT and plastic property characterization after degradation show that Sarocladium sp.SC23 related to the invention has great application potential in the aspect of waste plastic biodegradation.
Drawings
FIG. 1 purification scheme of strain SC23
FIG. 2 morphological observations of strain SC23 and 18S rRNA sequence phylogenetic tree
A: morphological characteristics of strain SC23, left panel is colony morphology observation; right panel is cytoscope observation (100×) b: strain SC2318S rRNA sequence phylogenetic tree analysis
FIG. 3 determination of degradation IMPRANIL DLN of strains and of the properties of PBA-PU
FIG. 4 measurement of degradation ability of polyurethane foam by Strain
A: weight loss ratio b of strain SC23 to polyurethane foam within 20 days: SEM image of polyurethane foam
FIG. 5 determination of the degradation ability of the strain to PBAT agricultural film
Biological material preservation information
Sarocladium sp.sc23, classified under the name Sarocladium sp, deposited at the collection of microorganism strains in Guangdong province, with a deposit address of the institute of microorganisms in Guangzhou province, 30 th floor 5 building, 5 th university, martyr, a deposit date of 2023, 6 months, 21 days, and a deposit number of GDMCC No:63578.
Detailed Description
EXAMPLE 1 isolation and purification of polyurethane Plastic degrading Strain
Collecting soil sample of Yichun needle leaf forest, sealing in a sealed plastic bag, and storing at-20deg.C. 0.25g of the collected soil sample of the needle forest in northeast China is weighed and placed in 100mL of inorganic salt culture medium (0.7g·L- 1K2HPO4,0.7g·L-1KH2PO4,0.7g·L-1MgSO4·7H2O,0.005g·L-1NaCl,1.34g·L-1NH4Cl,0.002g·L-1FeSO4·7H2O,0.002g·L-1ZnSO4·7H2O,0.001g·L-1MnSO4·H2O) containing 2g/L IMPRANIL DLN, and cultured for 7d at 30 ℃ and 180 rpm. Gradient dilution is carried out on the enrichment liquid according to 10 -1、10-2、10-3、10-4、10-5、10-6、10-7、10-8, the enrichment liquid is coated on a flat plate of an LB culture medium (yeast extract 5 g.L -1,10g·L-1 tryptone, 10 g.L -1 NaCl) and an inorganic salt culture medium 2g/LIMPRANIL DLN, and the enrichment liquid is cultured for 3-4 days at 30 ℃ to observe whether transparent rings are generated around colonies; single colonies with transparent circles were picked for streak purification and IMPRANIL DLN degradation function verification was performed on a petri dish containing 2g/L IMPRANIL DLN of inorganic salt solids (FIG. 1). As a result, it was found that one of the filamentous fungi degraded DLN to produce the largest transparent ring, which was designated as SC23.
Example 2 morphological observations and identification of polyurethane Plastic degrading Strain SC23
The separated and screened strain SC23 grows in mycelium shape on a culture dish containing 2g/L IMPRANIL DLN of inorganic salt solid, white spores grow in the center of a colony, and transparent rings are arranged around white mycelia at the edge of the colony; SC23 colonies grew in white and fungal hyphae on PDA medium (200 g.L -1 potato, 20 g.L -1 glucose, 15 g.L -1 agar) suitable for fungal culture; the observation under an oil microscope found that a large amount of mycelium and part of spores were produced during the growth of SC23 (fig. 2 a).
The 18S rRNA gene sequence of the strain was obtained by obtaining a genomic sketch of the strain, the obtained sequencing results were aligned with known sequences in the database by means of the NCBI (www.ncbi.nlm.nih.govPblastP) using BLAST software in the GenBank database, and the closest relatedness to Sarocladium sp was found by sequence alignment analysis (fig. 2 b). The strain was thus initially identified as Sarocladium sp.
Example 3 degradation Capacity of Strain pair IMPRANIL DLN and PBA-PU
In order to investigate whether the strain SC23 according to the invention is capable of degrading IMPRANIL DLN as well as PBA-PU. Strain SC23 (spore suspension of 1X 10 6, 5. Mu.L) was inoculated into oligotrophic plates (mineral salts medium with 5% LB) supplemented with 2g/L IMPRANIL DLN or 1g/L of PBA-PU, placed in a constant temperature incubator at 25℃and periodically observed for the production of transparent rings. After 3 days there was a clear visible transparent circle around the colony of strain SC23, indicating that strain SC23 can degrade substrate IMPRANIL DLN (fig. 3 a) and PBA-PU (fig. 3 b).
EXAMPLE 4 degradation ability of the Strain to polyurethane foam
In order to study whether the strain SC23 can degrade the actual polyurethane plastic products, the commercial polyester PUR foam is selected as an experimental material, and the degradation performance of the polyurethane plastic of the strain SC23 is determined by a plastic weight reduction experiment and the structural property change of the plastic after the strain treatment. In an inorganic salt medium containing 5% (v/v) LB, 6g/L of PUR foam was added, and a 2% spore suspension with a spore concentration of 1X 10 6 was inoculated, and incubated at 30℃and 180rpm, and after 5 days and 20 days the weight loss rates reached 30.41% and 94.17%, respectively (FIG. 4 a). The treated plastic was selected for structural characterization for 20 days, and strain treatment was found to significantly disrupt the plastic surface structure, the outer surface was eroded (fig. 4 b), and strain SC23 was presumed to be able to produce plastic degrading enzymes to disrupt the surface structure.
Example 5 degradation ability of the Strain on PBAT agricultural mulch
In order to study whether the strain SC23 provided by the invention can have degradation capability on an actual agricultural mulching film PBAT, the commercial PBAT agricultural mulching film is selected as an experimental material, and the degradation capability of the strain PBAT is determined through a plastic weight reduction experiment. As shown in FIG. 5, in the 5% (v/v) LB inorganic salt medium, adding 1g/L PBAT film, inoculating 2% strain SC23 spore suspension with spore concentration of 1X 10 6, 30 ℃,180rpm culture, 30 days later PBAT film in chip (figure 5 a), weight loss rate reached 37.86% (figure 5 b). The strain SC23 has good degradation capability on the PBAT film.
Claims (8)
1. The strain of the Cladosporium virens Sarocladium sp.SC23 which can degrade polyurethane plastics is preserved in the microorganism strain collection center of Guangdong province, the preservation address is the microbiological institute of the Guangdong province, the university of 100 No. 59 building 5 building Guangdong province academy of sciences of Guangzhou, city martyr, the preservation date is 2023, 6 and 21 days, and the preservation number is GDMCCNo:63578.
2. The culture, bacterial strain solution, bacterial strain fermentation broth or filtrate of fermentation broth of the culture of the Scopulariella furcifera SC23 of claim 1.
3. Culture, bacterial strain broth, bacterial strain fermentation broth or filtrate of fermentation broth according to claim 2, characterized in that the culture medium of the cladosporium virens SC23 is selected from the group consisting of LB medium, PDB medium, or medium supplemented with 2g/L IMPRANIL DLN of inorganic salts.
4. The biological agent comprising as an active ingredient the scoparia SC23 of claim 1.
5. The use of the culture, bacterial strain solution, bacterial strain fermentation solution or filtrate of fermentation broth of the scoparia SC23 of claim 1, the scoparia SC23 of claim 2, or the biological agent of claim 3 for degrading plastics.
6. The method according to claim 5, wherein the plastic is selected from IMPRANIL DLN, PBA-PU, polyurethane PUR plastic or agricultural mulch PBAT.
7. The method according to claim 6, wherein the polyurethane foam has a PUR concentration of 6g/L, a degradation condition of 30℃and 180rpm, and the seed solution has a seed concentration of 1X 10 6 at 2% spores inoculated with the strain of M.broomcorn SC23, and a degradation time of 20 days.
8. The use according to claim 6, wherein the PBAT concentration is 1g/L, the degradation condition is 30 ℃,180rpm, the seed solution of the strain of the Scoparia virens SC23 with 2% of spore concentration is 1X 10 6, and the degradation time is 30 days.
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| CN202410148108.9A CN118006469A (en) | 2024-02-01 | 2024-02-01 | Mucor pulmonale capable of degrading polyurethane plastic and application thereof |
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