CN117965384A - Degradation bacterium for liquefying and degrading kitchen waste, microbial inoculum containing degradation bacterium and application of degradation bacterium - Google Patents
Degradation bacterium for liquefying and degrading kitchen waste, microbial inoculum containing degradation bacterium and application of degradation bacterium Download PDFInfo
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- 238000006731 degradation reaction Methods 0.000 title abstract description 25
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- 150000001875 compounds Chemical class 0.000 claims abstract description 47
- 241000607715 Serratia marcescens Species 0.000 claims abstract description 36
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to the field of kitchen waste treatment, in particular to a degrading bacterium compound for liquefying and degrading kitchen waste and application thereof. The compound comprises 6 bacteria, namely, pseudomonas aphanidermatum, alcaligenes faecalis, paenibacillus amyloliquefaciens, serratia marcescens, sphingobacterium aquaticum and bacillus subtilis. The compound bacteria can be used for treating kitchen waste, so that protein, starch, cellulose and grease in the kitchen waste can be effectively degraded, and the degradation rates are 35.31%, 92.64%, 72.55% and 43.90% respectively. In addition, part of kitchen waste can be liquefied, so that the volume and weight of the kitchen waste can be effectively reduced, and the waste reduction rate of 99.78% is realized. In conclusion, the culture conditions of the compound bacteria are easy to realize and control, can effectively degrade and liquefy the kitchen waste, and have great significance for efficient treatment of the kitchen waste.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a degrading bacterium compound for liquefying and degrading kitchen waste and application thereof.
Background
Due to the rapid development of the catering industry, the amount of kitchen waste generated has rapidly increased in recent years. The kitchen waste has the characteristics of high yield, high pollutant concentration and the like, and if the kitchen waste is not properly treated, serious pollution is caused to the environment, and the normal life of part of organisms is influenced. The traditional kitchen waste treatment method comprises composting, landfill, incineration, animal feed preparation and the like, but has the characteristics of low cost, high efficiency and the like, but microorganisms cannot always directly utilize macromolecular substances in the kitchen waste, and if the kitchen waste is liquefied, the utilization rate of the microorganisms on nutrient substances in sewage can be obviously improved, and the subsequent purification and resource utilization are facilitated, so that the development of a method capable of rapidly degrading the liquefied kitchen waste is a key point of high-efficiency treatment of the kitchen waste.
Disclosure of Invention
The invention aims to provide degradation bacteria for liquefying and degrading kitchen waste, a microbial inoculum containing the degradation bacteria and application of the degradation bacteria.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a compound bacterium for liquefying and degrading kitchen waste, which comprises Serratia marcescens (SERRATIA MARCESCENS).
Further, the compound bacteria include Serratia marcescens (SERRATIA MARCESCENS) and Sphingobacterium aquaticum (Sphingobacterium mizutaii).
Further, the compound bacteria include candida utilis (Pseudochrobactrum asaccharolyticum), alcaligenes faecalis (ALCALIGENESFAECALIS), paenibacillus amyloliquefaciens (Paenibacillus amylolyticus), serratia marcescens (SERRATIA MARCESCENS), sphingosine-helper (Sphingobacteriummizutaii) and bacillus subtilis (Bacillus subtilis).
The invention also provides a microbial inoculum for liquefying and degrading kitchen waste, wherein the active ingredients of the microbial inoculum are the compound bacteria.
Further, the microbial inoculum is formed by mixing single-bacteria enrichment liquid of the bacillus caldus, the alcaligenes faecalis, the bacillus amyloliquefaciens, the Serratia marcescens, the Sphingobacterium aquaticum and the bacillus subtilis.
Further, the method comprises the steps of, the biomass of the bacteria including the non-saccharogenic pseudomonas, the alcaligenes faecalis, the paenibacillus amyloliquefaciens, the serratia marcescens, the sphingobacterium aquaticum and the bacillus subtilis is 1;1:1:1:1:1.
The application of the compound bacteria or the microbial inoculum in liquefying and degrading kitchen waste is also within the protection scope of the invention.
The invention also provides a kitchen waste liquefying and degrading method, which uses the compound bacteria or the microbial inoculum to inoculate the mixed liquid of the kitchen waste.
Further, the kitchen waste mixed solution is a mixture of solid waste and tap water, wherein the mass ratio of the solid waste to the tap water is 3:10 to 1:1
Further, after inoculating into the kitchen waste mixed solution, the initial biomass of the single bacteria of the non-sugar-decomposing pseudomonas, the alcaligenes faecalis, the paenibacillus amyloliquefaciens, the serratia marcescens, the sphingosine-on-water bacillus and the bacillus subtilis is 0.01-0.03 g/L, and the total biomass in the kitchen waste mixed solution is 0.06-0.18 g/L.
The invention provides a compound bacterium for degrading and liquefying kitchen waste and application thereof, wherein the compound bacterium comprises non-saccharogenic pseudomonas, alcaligenes faecalis, bacillus amyloliquefaciens, serratia marcescens, sphingobacterium aquaticum and bacillus subtilis, and Latin Pseudochrobactrum asaccharolyticum、Alcaligenes faecalis、Paenibacillus amylolyticus、Serratia marcescens、Sphingobacterium mizutaii、Bacillus subtilis. can effectively degrade protein, starch, cellulose and grease in the kitchen waste by utilizing the compound bacterium, can reduce the weight and volume of the kitchen waste, has the function of liquefying the kitchen waste, and has great significance for the subsequent recycling of the kitchen waste.
Preservation description
Biological material 1
Classification naming of biological materials: pseudomonas saccharolytica (Pseudochrobactrum asaccharolyticum).
Strain number of biological material: HL-1.
Preservation unit name of biological material: china general microbiological culture Collection center (China Committee for culture Collection).
The preservation unit of biological materials is abbreviated as: CGMCC.
Deposit unit address of biological material: north Star Xilu 1, 3 of the Chaoyang district of Beijing, the institute of microbiology, national academy of sciences, postal code: 100101.
Preservation date of biological material: 2023, 05, 23.
Accession numbers of the collection center of biological materials: CGMCC No.27442.
Biological material 2
Classification naming of biological materials: alcaligenes faecalis (ALCALIGENES FAECALIS).
Strain number of biological material: HL-2.
Preservation unit name of biological material: china general microbiological culture Collection center (China Committee for culture Collection).
The preservation unit of biological materials is abbreviated as: CGMCC.
Deposit unit address of biological material: north Star Xilu 1, 3 of the Chaoyang district of Beijing, the institute of microbiology, national academy of sciences, postal code: 100101.
Preservation date of biological material: 2023, 05, 23.
Accession numbers of the collection center of biological materials: CGMCC No.27443.
Biological material 3
Classification naming of biological materials: bacillus amyloliquefaciens (Paenibacillus amylolyticus).
Strain number of biological material: HL-4.
Preservation unit name of biological material: china general microbiological culture Collection center (China Committee for culture Collection).
The preservation unit of biological materials is abbreviated as: CGMCC.
Deposit unit address of biological material: north Star Xilu 1, 3 of the Chaoyang district of Beijing, the institute of microbiology, national academy of sciences, postal code: 100101.
Preservation date of biological material: 2023, 05, 23.
Accession numbers of the collection center of biological materials: CGMCC No.27445.
Biological material 4
Classification naming of biological materials: serratia marcescens (SERRATIA MARCESCENS).
Strain number of biological material: HL-18.
Preservation unit name of biological material: china general microbiological culture Collection center (China Committee for culture Collection).
The preservation unit of biological materials is abbreviated as: CGMCC.
Deposit unit address of biological material: north Star Xilu 1, 3 of the Chaoyang district of Beijing, the institute of microbiology, national academy of sciences, postal code: 100101.
Preservation date of biological material: 2023, 05, 23.
Accession numbers of the collection center of biological materials: CGMCC No.27448.
Biological material 5
Classification naming of biological materials: sphingobacterium aquaticum (Sphingobacterium mizutaii).
Strain number of biological material: HL-26.
Preservation unit name of biological material: china general microbiological culture Collection center (China Committee for culture Collection).
The preservation unit of biological materials is abbreviated as: CGMCC.
Deposit unit address of biological material: north Star Xilu 1, 3 of the Chaoyang district of Beijing, the institute of microbiology, national academy of sciences, postal code: 100101.
Preservation date of biological material: 2023, 05, 23.
Accession numbers of the collection center of biological materials: CGMCC No.27451.
Biological material 6
Classification naming of biological materials: bacillus subtilis (Bacillus subtilis).
Strain number of biological material: HL-27.
Preservation unit name of biological material: china general microbiological culture Collection center (China Committee for culture Collection).
The preservation unit of biological materials is abbreviated as: CGMCC.
Deposit unit address of biological material: north Star Xilu 1, 3 of the Chaoyang district of Beijing, the institute of microbiology, national academy of sciences, postal code: 100101.
Preservation date of biological material: 2023, 05, 23.
Accession numbers of the collection center of biological materials: CGMCC No.27452.
Drawings
FIG. 1 shows the maximum degradation rates of protein (a), cellulose (b), lipid (c) and starch (d) by a single strain in example 2.
FIG. 2 shows the degradation rate of protein, starch, cellulose and grease in example 2.
Fig. 3 is a reduction rate of the weight and volume of the kitchen waste in example 2.
Fig. 4 shows the reduction rate of the height of kitchen waste after being degraded by the compound bacteria for one night (14 h) in the embodiment 3 (the abscissa of the figure 1-6 is the time point of the height of the waste respectively).
Fig. 5 shows the removal rate of COD (COD removal rate versus time) of the kitchen waste liquefaction liquid effluent after the kitchen waste is degraded by the compound bacteria for one night (14 h) in example 3.
Detailed Description
The following detailed description of the invention is provided in connection with the accompanying drawings that are presented to illustrate the invention and not to limit the scope thereof. The examples provided below are intended as guidelines for further modifications by one of ordinary skill in the art and are not to be construed as limiting the invention in any way.
The experimental methods in the following examples, unless otherwise specified, are conventional methods, and are carried out according to techniques or conditions described in the literature in the field or according to the product specifications. Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
The invention provides a compound bacterium for degrading and liquefying kitchen waste and application thereof, wherein the compound bacterium comprises pseudomonas solanacearum, alcaligenes faecalis, paenibacillus amyloliquefaciens, serratia marcescens, sphingobacterium aquaticum, bacillus subtilis and Latin Pseudochrobactrum asaccharolyticum、Alcaligenesfaecalis、Paenibacillus amylolyticus、Serratia marcescens、Sphingobacterium mizutaii、Bacillus subtilis.
The various strains in the invention can be obtained by conventional separation and screening means.
The invention also provides application of the compound bacteria in degrading liquefied kitchen waste.
In the invention, the compound bacteria are inoculated into the kitchen waste mixed liquid.
In the present invention, the initial biomass of 6 bacteria in the complex bacteria is the same, preferably 0.01 to 0.03g/L, more preferably 0.02g/L, and the total initial biomass is 0.06 to 0.18g/L, more preferably 0.12g/L.
In the invention, the mass ratio of the solid waste to tap water in the kitchen waste mixed solution is preferably 3:10 to 1:1, further preferably 3:5.
In the present invention, the degradation process is performed in a 100mL Erlenmeyer flask, which is placed in a constant temperature shaker at a rotation speed of preferably 125-135 r/min, more preferably 130r/min.
In the present invention, the degradation temperature is preferably 25 to 35 ℃, and more preferably 30 ℃.
In the present invention, the degradation period is preferably 5 to 7d, more preferably 6d.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
The materials used in the examples are as follows:
1. Enrichment medium: 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 1000mL of distilled water and pH 7.2-7.4.
2. Kitchen waste mixed solution: kitchen waste is taken from a dining hall of a college, and after bulk solid waste is removed, the kitchen waste is processed according to the following steps: tap water = 3:5 (mass ratio) mixing the kitchen waste with tap water to obtain the kitchen waste mixed solution.
Example 1
The strain with degradation effects of protein, starch, cellulose, grease and the like is sequenced on a Hiseq 2500 platform. Sequencing to obtain bacterial 16S rRNA gene sequences in each hole, performing Blast comparison at NCBI to obtain a comparison strain of each bacterium, and performing patent program preservation, wherein specific information is as follows.
Wherein the 16S rRNA gene of the bacterium having the number HL-1 has the sequence shown in the sequence 1, and is identified as the Pseudomonas saccharolytica. The bacteria are in a rod shape and are distributed. The colony is white, has smaller shape, round shape, moist, raised surface, transparent, greasy, glossy and neat edge. Bacteria were not capsular, not spore, and gram staining was negative. The registration number of the non-sugar-decomposing pseudomonas (Pseudochrobactrum asaccharolyticum) HL-1 in the common microorganism center of the China Committee for culture Collection of microorganisms is CGMCC No.27442. The strain is preserved in China general microbiological culture collection center (CGMCC) for 5 months and 23 days in 2023, and the preservation address is North Star Xiu No. 1 and No. 3 in the Korean area of Beijing city. Hereinafter referred to as sugar-free pseudo-pale Bai Ganjun HL-1.
Wherein the 16S rRNA gene of the bacterium numbered HL-2 has a sequence as shown in sequence 2 and is identified as Alcaligenes faecalis. Gram-negative bacilli, approximately 0.7-1.0 μm in diameter, and periflagellum movement. Culturing on nitrogen-free plate culture medium with benzoate as carbon source at 30deg.C for 5 days to obtain colony with round shape, smooth surface, raised edge, and 1-1.5mm diameter, and melanin production. Respiratory metabolism, oxidase positivity, and no carbohydrate growth.
Alcaligenes faecalis (ALCALIGENES FAECALIS) HL-2, which has a registration number of CGMCC No.27443 in the China general microbiological culture Collection center. The strain is preserved in China general microbiological culture collection center (CGMCC) for 5 months and 23 days in 2023, and the preservation address is North Star Xiu No. 1 and No. 3 in the Korean area of Beijing city. Hereinafter referred to as Alcaligenes faecalis HL-2.
Wherein the 16S rRNA gene of the bacterium having the number HL-4 was identified as Bacillus amyloliquefaciens as shown in the sequence 4. Gram positive bacteria, the colony is pale yellow, has smooth surface, is not moist, has irregular edges and semitransparent, and has the diameter of about 1mm.
Bacillus amyloliquefaciens (Paenibacillus amylolyticus) HL-4 has a registration number of CGMCC No.27445 in the China general microbiological culture Collection center. The strain is preserved in China general microbiological culture collection center (CGMCC) for 5 months and 23 days in 2023, and the preservation address is North Star Xiu No. 1 and No. 3 in the Korean area of Beijing city. Hereinafter, bacillus amyloliquefaciens HL-4 is abbreviated.
Wherein the 16S rRNA gene of the bacterium designated HL-18 has a sequence as shown in sequence 7, and is identified as Serratia marcescens. The colonies of the strain were essentially convex, opaque in the center, irregular in the edges, and 1 to 2.5mm in size, all producing a red pigment (Panel I-3). Particular colonies (Panel I-1) can be generated on nutrient agar plates in a dendritic state with left-hand rotation; under specific conditions, double-row colonies (plate I-2) can appear which are successively ordered from small to large. The bacterial cells are gram-negative short bacillus, the size is (1-1.3) mu m x (0.7-1.0) mu m, and the bacterial cells are periphytic flagellum, dynamic, non-capsular and non-spore.
Serratia marcescens (SERRATIA MARCESCENS) HL-18 has a registration number of CGMCC No.27448 in the China general microbiological culture Collection center. The strain is preserved in China general microbiological culture collection center (CGMCC) for 5 months and 23 days in 2023, and the preservation address is North Star Xiu No. 1 and No. 3 in the Korean area of Beijing city. Serratia marcescens HL-18 is hereinafter abbreviated.
Wherein the 16S rRNA gene of the bacterium designated HL-26 has a sequence as shown in SEQ ID No. 10, and is identified as Sphingobacterium aquaticum. The cells are in a short rod shape, the width is 0.2-0.7 mu m, and the length is 1.0-2.5 mu m; gram-negative bacteria, do not move and do not produce spores.
Sphingobacterium hydrobromide (Sphingobacterium mizutaii) HL-26, which has a registration number of CGMCC No.27451 in the China general microbiological culture Collection center. The strain is preserved in China general microbiological culture collection center (CGMCC) for 5 months and 23 days in 2023, and the preservation address is North Star Xiu No. 1 and No. 3 in the Korean area of Beijing city. The Sphingobacterium aquaticum HL-26 is hereinafter abbreviated.
Wherein the 16S rRNA gene of the bacterium designated HL-27 has a sequence as shown in sequence 11, and is identified as Bacillus subtilis. The single cell is 0.7-0.8X2-3 microns and the coloring is uniform. Without capsule, the flagellum can be grown and can move. Gram positive bacteria can form endogenous stress-resistant spores, the spores are 0.6-0.9X1.0-1.5 microns, ellipse is columnar, and the spores are positioned in the center or slightly deviated from the bacteria, so that the bacteria do not expand after the spores are formed. The growth and propagation speed is high, the colony surface is rough and opaque, the pollution is white or yellowish, and when the colony grows in a liquid culture medium, the colony surface often forms wrinkling, so the colony is an aerobic bacterium.
Bacillus subtilis (Bacillus subtilis) HL-27 with registration number of CGMCC No.27452 in China general microbiological culture Collection center. The strain is preserved in China general microbiological culture collection center (CGMCC) for 5 months and 23 days in 2023, and the preservation address is North Star Xiu No.1 and No. 3 in the Korean area of Beijing city. Hereinafter, bacillus subtilis HL-27 is abbreviated.
Example 2
1. Degradation effect of single strain
The 6 strains in example 1 were respectively expanded to a liquid medium, and the initial biomass of 0.05g/L was inoculated into a protein, starch, cellulose and lipid screening medium, and the cellulose, protein, starch and lipid contents of 0 and 6d were respectively measured, and the degradation rates of the above substances by the different strains were calculated, and the results are shown in FIG. 1. In FIG. 1, strains corresponding to different numbers on the abscissa are shown in Table 1.
Referring to FIG. 1 and Table 1, the maximum degradation rates of a single strain on protein, cellulose, oil and starch were 35.59%, 45.50%, 94.34%, 99.71%, and the corresponding strains were SERRATIA MARCESCENS (Serratia marcescens), sphingobacterium mizutaii (Sphingobacterium hydrobromide), paenibacillus amylolyticus (Bacillus amyloliquefaciens), paenibacillus amylolyticus (Bacillus amyloliquefaciens), respectively.
TABLE 1 strain numbering and name
2. Degradation effect of composite strain
Placing 30g of kitchen waste and 50g of tap water into a 100mL conical flask, uniformly mixing to obtain a single-bacteria enrichment solution of six bacteria of the non-sugar-decomposing pseudopallium HL-1, the alcaligenes faecalis HL-2, the paenibacillus amyloliquefaciens HL-4, the serratia marcescens HL-18, the sphingosine-motor HL-26 and the bacillus subtilis HL-27, and adding the single-bacteria enrichment solution into the kitchen waste mixed solution according to the initial biomass of 0.02g/L, namely 1L of the kitchen waste mixed solution inoculated with the microbial inoculum, wherein the single-bacteria enrichment solution contains 0.02g of each of the non-sugar-decomposing pseudopallium, the alcaligenes faecalis, the paenibacillus amyloliquefaciens, the serratia marcescens, the sphingosine-motor and the bacillus subtilis. The conical flask was placed in a thermostatic water bath shaker at 30℃and 130r/min for 6 days.
At 0d (before the start of the reaction) and 6d, the protein, starch, cellulose, and lipid contents measured were sampled, respectively. The results are shown in fig. 2, and it can be seen from fig. 2 that the compound bacteria can effectively remove protein, starch, cellulose and grease in the kitchen waste mixed solution, and the removal rates are 35.31%, 92.64%, 72.55% and 43.90+/-3.55% respectively.
At the 0d (before the reaction starts) and the 6d, the weight and the volume of the solid waste 2 in the 2 kitchen waste mixed solution are respectively sampled and measured. As shown in fig. 3, it can be seen from fig. 3 that the compound bacteria can effectively liquefy kitchen waste, effectively reduce volume and weight, and the weight reduction rate and the volume reduction rate before and after liquefaction are respectively 54.95% and 63.99%.
Example 3
Aiming at the problem that the kitchen waste is difficult to degrade at present, the invention provides a pilot scale process and a method for applying compound bacteria (compound bacteria consisting of six bacterial agents described in example 2) to degradation of the kitchen waste. The compound bacteria are applied to kitchen waste biochemical treatment aerobic fermentation equipment to treat kitchen waste in restaurants of municipal companies in Beijing city, and the system achieves the aim of remarkably degrading the kitchen waste. And the operation parameters of the equipment are adjusted, so that the function of the compound bacteria is exerted to the maximum extent. The total feeding amount of the kitchen waste can reach 469.18kg within 7 days, the waste is degraded by compound bacteria after entering the equipment (0.02 g of each liter of mixed liquor of the kitchen waste is inoculated with the pseudomonas solanacearum, the alcaligenes faecalis, the paenibacillus amyloliquefaciens, the serratia marcescens, the sphingosine-b and the bacillus subtilis), and the waste reduction rate can reach 99.78 percent. The calculation formula of the kitchen waste decrement rate is as follows: kitchen waste reduction rate=1- (1.05/469.18) =99.78% (note: parameter 1.05 is mass of undegraded cellulose in the waste treatment equipment after 7 days).
The compound bacteria of the invention are applied to a pilot-scale process for one week, and the specific operation steps are as follows:
(1) Firstly, performing expansion culture of a compound bacterial liquid laboratory stage, and applying the compound bacterial liquid laboratory stage to pilot plant test process equipment;
(2) Adding the compound bacterial liquid into garbage treatment equipment, adding bacterial attachment materials, and adjusting and optimizing equipment parameters (such as spraying time and the like);
(3) Measuring the feeding amount of garbage every time, observing the height change condition of kitchen garbage in the equipment, collecting the effluent before and after garbage feeding, and detecting the effluent quality.
As shown in fig. 4 and 5, after one-night degradation (14 h), the height of the garbage is obviously reduced, the maximum reduction rate of the height of the garbage can reach 21.40%, the height of the garbage shows a stable fluctuation trend in the same day, and the effective reduction of kitchen garbage is realized. Meanwhile, after the compound bacteria degrade kitchen waste, the COD removal rate of the obtained effluent liquefied liquid can reach 93.26 percent at the highest. The parameters of the garbage treatment equipment are optimized, the performance of the compound bacterial liquid is obviously improved, the viscosity change of the garbage liquefied liquid is reflected, and the degradation performance of garbage is also improved.
In conclusion, the compound bacteria can effectively degrade and liquefy the kitchen waste.
From the above examples, the invention provides a compound bacterium for degrading liquefied kitchen waste and application thereof, wherein the compound bacterium comprises 6 bacteria, namely, pseudomonas solanacearum, alcaligenes faecalis, paenibacillus amyloliquefaciens, serratia marcescens, sphingosine-b-water and bacillus subtilis, and Latin Pseudochrobactrum asaccharolyticum、Alcaligenesfaecalis、Paenibacillus amylolyticus、Serratia marcescens、Sphingobacterium mizutaii、Bacillus subtilis., and the compound bacterium can be used for treating the kitchen waste, so that protein, starch, cellulose and grease in the kitchen waste can be effectively degraded, and the degradation rates are 35.31%, 92.64%, 72.55% and 43.90% respectively. In addition, part of kitchen waste can be liquefied, so that the volume and weight of the kitchen waste can be effectively reduced, and the weight reduction rate and the volume reduction rate before and after liquefaction are respectively 54.95% and 63.99%. In practical pilot-plant application, the compound bacteria can still realize a good continuous degradation effect on kitchen waste, and the waste reduction rate in one week can reach 99.78%. The culture condition of the compound bacteria is easy to realize and control, can effectively degrade and liquefy the kitchen waste, and has great significance for the efficient treatment of the kitchen waste.
The present application is described in detail above. It will be apparent to those skilled in the art that the present application can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the application and without undue experimentation. While the application has been described with respect to specific embodiments, it will be appreciated that the application may be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the application following, in general, the principles of the application and including such departures from the present disclosure as come within known or customary practice within the art to which the application pertains. The application of some of the basic features may be done in accordance with the scope of the claims that follow.
Claims (10)
1. The compound bacteria for liquefying and degrading kitchen waste is characterized by comprising Serratia marcescens (SERRATIA MARCESCENS).
2. The compound bacterium according to claim 1, wherein the compound bacterium comprises serratia marcescens (SERRATIA MARCESCENS) and sphingobacterium aquaticum (Sphingobacterium mizutaii).
3. The compound bacterium according to claim 2, wherein the compound bacterium comprises pseudomonas saccharolytica (Pseudochrobactrum asaccharolyticum), alcaligenes faecalis (ALCALIGENESFAECALIS), paenibacillus amyloliquefaciens (Paenibacillus amylolyticus), serratia marcescens (SERRATIA MARCESCENS), sphingosine-helper (Sphingobacterium mizutaii) and bacillus subtilis (Bacillus subtilis).
4. The microbial inoculum for liquefying and degrading kitchen waste is characterized in that the active ingredient of the microbial inoculum is the compound bacteria in any one of claims 1-3.
5. The microbial inoculum according to claim 4, wherein the microbial inoculum is formed by mixing a single-bacteria-enriched liquid of pseudomonas saccharolytica, alcaligenes faecalis, paenibacillus amyloliquefaciens, serratia marcescens, sphingobacterium aquaticum and bacillus subtilis.
6. The microbial inoculum according to claim 5, wherein the biomass of the bacteria such as pseudomonas saccharolytica, alcaligenes faecalis, paenibacillus amyloliquefaciens, serratia marcescens, sphingobacterium aquaticum and bacillus subtilis is 1;1:1:1:1:1.
7. The use of the compound bacteria of any one of claims 1-3 or the microbial inoculum of any one of claims 4-6 in liquefying and degrading kitchen waste.
8. The kitchen waste liquefying and degrading method is characterized in that the compound bacteria in any one of claims 1-3 or the microbial inoculum in any one of claims 4-6 is used for inoculating into the kitchen waste mixed liquid.
9. The method according to claim 8, wherein the kitchen waste mixed liquor is a mixture of solid waste and tap water, and the mass ratio of the solid waste to the tap water is 3:10 to 1:1.
10. The method of claim 9, wherein the initial biomass of the single bacteria of the species of the genus pseudomonas, alcaligenes faecalis, paenibacillus amyloliquefaciens, serratia marcescens, sphingosine-helper and bacillus subtilis is 0.01-0.03 g/L after inoculation into the mixed liquid of kitchen waste, and the total biomass in the mixed liquid of kitchen waste is 0.06-0.18 g/L.
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