CN117965281B - Sampling device and sampling method for saline-alkali soil alfalfa rhizosphere microorganisms - Google Patents
Sampling device and sampling method for saline-alkali soil alfalfa rhizosphere microorganisms Download PDFInfo
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- CN117965281B CN117965281B CN202410387210.4A CN202410387210A CN117965281B CN 117965281 B CN117965281 B CN 117965281B CN 202410387210 A CN202410387210 A CN 202410387210A CN 117965281 B CN117965281 B CN 117965281B
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- 238000005070 sampling Methods 0.000 title claims abstract description 134
- 239000002689 soil Substances 0.000 title claims abstract description 59
- 244000005700 microbiome Species 0.000 title claims abstract description 32
- 241000219823 Medicago Species 0.000 title claims abstract description 30
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 title claims abstract description 30
- 239000003513 alkali Substances 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 11
- 241000196324 Embryophyta Species 0.000 abstract description 8
- 238000003466 welding Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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Abstract
The invention is suitable for the technical field of plant rhizosphere microorganism analysis, and provides a sampling device and a sampling method for saline-alkali soil alfalfa rhizosphere microorganisms.
Description
Technical Field
The invention belongs to the technical field of plant rhizosphere microorganism analysis, and particularly relates to a sampling device and a sampling method for saline-alkali soil alfalfa rhizosphere microorganisms.
Background
Rhizosphere microorganisms are microorganisms closely adhered to rhizosphere soil particles, bacteria are taken as a main material, moisture conditions and ventilation conditions of the rhizosphere are better than those of non-rhizosphere, a microenvironment which is favorable for growth and propagation of the rhizosphere microorganisms is formed, the rhizosphere microorganisms have profound effects on various aspects such as plant growth, nutrition and health, so that more and more rhizosphere microorganisms are researched, for example, saline-alkali soil alfalfa rhizosphere microorganisms which are researched at present are researched, and for research, the rhizosphere microorganisms need to be sampled.
At present, some sampling modes are to pull up and collect whole plants, which can destroy the whole experimental environment, and the document with the application number of CN114134020A relates to a nondestructive sampling device and a nondestructive sampling method for plant rhizosphere microorganisms. The plant material can be prevented from being damaged, so that the same plant can not be changed by rhizosphere microorganisms caused by root system damage, and the same plant can be sampled at multiple time points; and can quantitative sampling, the sample is more convenient quick.
Above-mentioned prior art is through setting up inlayer netted container and round outer netted container, takes out the outer netted container and takes out the sample, and on the one hand, outer netted container quantity is limited, and the sampling scope is limited, and on the other hand, outer netted container is vertical buries, only can carry out the ascending sample of vertical direction, and the sampling angle is comparatively single.
Disclosure of Invention
The invention provides a sampling device for saline-alkali soil alfalfa rhizosphere microorganisms, and aims to solve the problems.
The invention is realized in such a way that a sampling device for saline-alkali soil alfalfa rhizosphere microorganisms comprises:
A frame;
A planting pot arranged on the frame, which is filled with saline-alkali soil for planting alfalfa;
The sampling unit is arranged on the frame and used for acquiring the root system of the alfalfa;
The middle positions of the tops of the two opposite side walls of the planting pot are respectively provided with a crankshaft in a rotating way, the crankshafts are in rotating connection with a rack, a fourth stepping motor for driving the crankshafts to rotate is fixed on the rack, and a plurality of first through holes are formed in the side walls and the bottom of the planting pot in a circumferential spacing way;
The sampling unit includes:
The first annular plate is arranged outside the planting pot and rotatably mounted on the frame, a first stepping motor for driving the first annular plate to rotate on a horizontal plane is fixed on the frame, vertical third telescopic rods are fixed at the bottoms of the front side wall and the rear side wall of the first annular plate, and supporting plates are fixed at the telescopic ends of the third telescopic rods and used for supporting the bottoms of the planting pot;
the second annular plate is coaxially and rotatably arranged on the first annular plate, and the first annular plate is provided with a power structure for driving the second annular plate to rotate on a vertical surface;
At least one sampling member mounted on the second ring plate.
Preferably, the power structure comprises:
the first gear is rotatably arranged on the first annular plate, and a fifth stepping motor for driving the first gear to rotate is fixed on the first annular plate;
And the first gear is fixed on the second annular plate and meshed with the first gear.
Preferably, the frame comprises:
a bottom plate;
The two side plates are symmetrically fixed on the bottom plate, and the crankshaft is rotationally connected with the side plates.
Preferably, the lower part of the first annular plate is symmetrically fixed with supporting legs which are in sliding connection with an annular chute arranged on the upper surface of the bottom plate.
Preferably, the outside cover of planting the basin is equipped with the rubber sleeve, the rubber sleeve top is less than the top of planting the basin, the second through-hole has all been seted up in the rubber sleeve top that is located every first through-hole outside, the diameter of second through-hole is less than the diameter of first through-hole, and the interval of two adjacent first through-holes on the same circumference is greater than the diameter of second through-hole, the rubber sleeve outside is equipped with the frame, the frame top rotates with planting basin lateral wall to be connected, frame and rubber sleeve fixed connection, install the driving piece that is used for driving frame pivoted on the planting basin.
Preferably, the frame comprises a plurality of connecting rings which are arranged at intervals from top to bottom, and a plurality of vertical connecting rods are fixedly connected between the connecting rings at intervals in the circumferential direction.
Preferably, the driving member includes:
a third stepping motor fixed at the top of the planting pot;
and a second gear fixed on the output shaft of the third stepping motor, and a second gear ring meshed with the second gear is fixed on the frame.
Preferably, the sampling part includes:
The cylinder shell is connected with the second annular plate through a first telescopic rod and a second telescopic rod, the first telescopic rod and the second telescopic rod are symmetrically arranged on the axis of the cylinder shell, one end of the first telescopic rod is fixedly connected with the second annular plate, the other end of the first telescopic rod is hinged with a sliding block, the sliding block is in sliding connection with the side wall of the cylinder shell, one end of the second telescopic rod is fixedly connected with the second annular plate, the other end of the second telescopic rod is hinged with the side wall of the cylinder shell, and a plurality of shell doors are arranged on the cylinder shell;
the ring cutter sampling tube is coaxially arranged with the cylindrical shell and penetrates through the cylindrical shell, the other end of the ring cutter sampling tube, which is close to the opening at one end of the planting pot, is closed, the ring cutter sampling tube is rotationally connected with the cylindrical shell, and the outer diameter of the ring cutter sampling tube is smaller than the diameter of the second through hole;
the power piece is arranged on the outer side wall of the cylindrical shell and used for driving the ring cutter sampling cylinder to rotate;
the auger is rotatably arranged in the ring cutter sampling cylinder, and a second stepping motor for driving the auger to rotate is fixed at the other end of the ring cutter sampling cylinder;
The cylindrical shell is internally provided with a cavity, the cavity is obtained by rotary cutting of an isosceles trapezoid around a ring cutter sampling tube, the lower bottom of the isosceles trapezoid coincides with the outer wall of the ring cutter sampling tube, isosceles trapezoid rotating plates matched with the cavity are respectively fixed on two side walls of the ring cutter sampling tube, the angle between the two rotating plates is 180 degrees, and a soil outlet is formed between the two rotating plates on the wall of the ring cutter sampling tube.
The invention also provides a sampling method based on the sampling device for the saline-alkali soil alfalfa rhizosphere microorganisms, which comprises the following steps:
the second annular plate is driven to rotate on the vertical surface through the power structure, and the second annular plate drives the sampling component to move on the vertical surface;
The first annular plate is driven to rotate on the horizontal plane by the first stepping motor, so that the sampling component is driven to move on the horizontal plane, and the position of the sampling component is adjusted;
the sampling part enters the planting pot through the first through hole to obtain a root system.
Compared with the prior art, the embodiment of the application has the following main beneficial effects:
The sampling device for the saline-alkali soil alfalfa rhizosphere microorganisms is characterized in that a frame, a planting basin and a sampling unit are arranged, the sampling unit comprises a first annular plate, a first stepping motor, a second annular plate, a power structure and a sampling part, the second annular plate is driven to rotate on a vertical surface through the power structure, the second annular plate drives the sampling part to move on the vertical surface, the first annular plate is driven to rotate on a horizontal surface through the first stepping motor, so that the sampling part is driven to move on the horizontal surface, the position of the sampling part is adjusted, then the sampling part enters the planting basin through a first through hole, a root system is obtained, and sampling can be carried out from different directions and positions at the top, the bottom and the side edges of the planting basin, and more sampling requirements are met.
Drawings
FIG. 1 is a schematic structural diagram of a sampling device for saline-alkali soil alfalfa rhizosphere microorganisms;
FIG. 2 is a schematic installation diagram of a planting pot in a sampling device for saline-alkali soil alfalfa rhizosphere microorganisms;
FIG. 3 is a schematic structural view of a planting pot in a sampling device for saline-alkali soil alfalfa rhizosphere microorganisms;
Fig. 4 is an enlarged view at a in fig. 1;
Fig. 5 is a schematic structural view of a cylindrical shell in a sampling device for saline-alkali soil alfalfa rhizosphere microorganisms.
Reference numerals annotate: 1. a bottom plate; 2. support legs; 3. a first stepping motor; 4. a first annular plate; 5. a second annular plate; 6. a cylindrical housing; 7. a first telescopic rod; 8. a first gear; 9. a second stepping motor; 10. a power member; 11. a second telescopic rod; 12. a ring cutter sampling tube; 13. an auger; 14. a second ring gear; 15. planting pot; 16. a side plate; 17. a crankshaft; 18. a third stepper motor; 19. a second gear; 20. a frame; 21. a rubber sleeve; 22. a second through hole; 23. a first through hole; 24. a support plate; 25. a third telescopic rod; 26. a fourth stepping motor; 27. a shell door; 28. a rotating plate; 29. a soil outlet; 30. a chamber.
Detailed Description
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the description of the applications herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having" and any variations thereof in the description of the application and the claims and the description of the drawings above are intended to cover a non-exclusive inclusion. The terms first, second and the like in the description and in the claims or in the above-described figures, are used for distinguishing between different objects and not necessarily for describing a sequential or chronological order.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
The embodiment of the invention provides a sampling device for saline-alkali soil alfalfa rhizosphere microorganisms, which is shown in fig. 1-5 and comprises:
A frame;
a planting pot 15 mounted on the frame, which is filled with saline-alkali soil for planting alfalfa;
The sampling unit is arranged on the frame and used for acquiring the root system of the alfalfa;
the middle positions of the tops of the two opposite side walls of the planting pot 15 are respectively provided with a crankshaft 17 in a rotating way, the crankshafts 17 are rotationally connected with a frame, a fourth stepping motor 26 for driving the crankshafts 17 to rotate is fixed on the frame and can be fixed through bolts, and a plurality of first through holes 23 are formed in the side walls and the bottoms of the planting pot 15 in a circumferential spacing way;
The sampling unit includes:
The first annular plate 4 is arranged outside the planting pot 15, the first annular plate 4 is rotatably arranged on a frame, a first stepping motor 3 for driving the first annular plate 4 to rotate on a horizontal plane is fixed on the frame, the first annular plate 4 can be fixed through bolts, vertical third telescopic rods 25 are fixed at the bottoms of the front side wall and the rear side wall of the first annular plate 4, the telescopic ends of the third telescopic rods 25 are fixed through screws, a supporting plate 24 is fixed at the telescopic ends of the third telescopic rods 25 and used for supporting the bottom of the planting pot 15, and the rotation of the planting pot 15 can be limited through the supporting plate 24 during sampling;
The second annular plate 5 is coaxially and rotatably arranged on the first annular plate 4, the first annular plate 4 is provided with a power structure for driving the second annular plate 5 to rotate on a vertical surface, and of course, the first annular plate 4 and the second annular plate 5 can be driven to rotate in a manual driving mode without excessive limitation;
at least one sampling member mounted on the second ring plate 5.
During sampling, the second annular plate 5 is driven to rotate on the vertical surface through the power structure, the second annular plate 5 drives the sampling component to move on the vertical surface, the first annular plate 4 is driven to rotate on the horizontal surface through the first stepping motor 3, so that the sampling component is driven to move on the horizontal surface, the position of the sampling component is adjusted, then the sampling component enters the planting basin 15 through the first through hole 23, a root system is obtained, sampling can be carried out from different directions and positions of the top, the bottom and the side edges of the planting basin 15, more sampling requirements are met, when sampling is carried out on the positions of the front side wall and the rear side wall of the planting basin 15, the crankshaft 17 is driven to rotate to a horizontal state through the fourth stepping motor 26, and the bending space of the crankshaft 17 can be used for the first annular plate 4 to enter, so that sampling is not influenced.
Wherein, the power structure includes:
The first gear 8 is rotatably arranged on the first annular plate 4, a fifth stepping motor for driving the first gear 8 to rotate is fixed on the first annular plate 4, and the fifth stepping motor can be fixed through bolts;
The first gear ring fixed on the second annular plate 5 can be fixed by welding, and the first gear 8 is meshed with the first gear ring;
The first gear 8 is driven to rotate by the fifth stepping motor, and the first gear 8 drives the second annular plate 5 to rotate by the first gear ring.
Specifically, the frame includes:
A base plate 1;
Two side plates 16 symmetrically fixed on the bottom plate 1 can be fixed by welding, and the crankshaft 17 is rotationally connected with the side plates 16;
Furthermore, the lower part of the first annular plate 4 is symmetrically fixed with supporting legs 2, the supporting legs 2 are in sliding connection with an annular chute formed in the upper surface of the bottom plate 1, and the first annular plate 4 is supported by the two supporting legs 2, so that the stability of the first annular plate 4 in rotation is improved;
In this embodiment, the outer part of the planting pot 15 is sleeved with a rubber sleeve 21, the top of the rubber sleeve 21 is lower than the top of the planting pot 15, the outer side of each first through hole 23 on the rubber sleeve 21 is provided with a second through hole 22, the diameter of each second through hole 22 is smaller than that of each first through hole 23, the distance between two adjacent first through holes 23 on the same circumference is larger than that of each second through hole 22, a frame 20 is arranged outside the rubber sleeve 21, the top of the frame 20 is rotationally connected with the outer side wall of the planting pot 15, the frame 20 is rotationally connected with the rubber sleeve 21 through a bearing, and a driving piece for driving the frame 20 to rotate is arranged on the planting pot 15;
It can be understood that the driving piece drives the frame 20 to rotate by a set angle, the frame 20 drives the rubber sleeve 21 to rotate, so that the second through hole 22 on the rubber sleeve 21 is dislocated with the first through hole 23 on the planting pot 15, the first through hole 23 is closed, root systems are prevented from growing outside the planting pot 15 from the first through hole 23 in the planting process, and meanwhile, moisture in soil is prevented from directly losing from the first through hole 23;
in a specific implementation, the frame 20 includes a plurality of connection rings that are disposed at intervals from top to bottom, the connection rings can be fixedly connected with the rubber sleeve 21 through glue or screws, a plurality of vertical connection rods are fixedly connected between the plurality of connection rings at intervals in a circumferential direction, and the connection rings can be fixed through welding, so that the structure of the frame 20 does not interfere with sampling and does not have excessive limitation;
Still further, the driving member includes:
a third stepping motor 18 fixed on the top of the planting pot 15, which can be fixed by screws;
A second gear 19 fixed on the output shaft of the third stepping motor 18, and a second gear ring 14 meshed with the second gear 19 is fixed on the frame 20, and can be fixed by welding;
the third stepping motor 18 drives the second gear 19 to rotate by a set angle, the second gear 19 drives the frame 20 to rotate through the second gear ring 14, and the frame 20 drives the rubber sleeve 21 to rotate.
In this embodiment, the sampling part includes:
The cylindrical shell 6, the cylindrical shell 6 is connected with the second annular plate 5 through a first telescopic rod 7 and a second telescopic rod 11, the first telescopic rod 7 and the second telescopic rod 11 are symmetrically arranged on the axis of the cylindrical shell 6, one end of the first telescopic rod 7 is fixedly connected with the second annular plate 5 and can be fixed by screws, the other end of the first telescopic rod 7 is hinged with a sliding block, the sliding block is slidably connected with the side wall of the cylindrical shell 6, one end of the second telescopic rod 11 is fixedly connected with the second annular plate 5, the other end of the second telescopic rod 11 is hinged with the side wall of the cylindrical shell 6, preferably the cylindrical shell 6 is made of transparent materials, and a plurality of shell doors 27 are arranged on the cylindrical shell 6 to facilitate the opening and the taking out of soil or root systems;
The ring cutter sampling tube 12 is coaxially arranged with the cylindrical shell 6 and penetrates through the cylindrical shell 6, one end of the ring cutter sampling tube 12 close to the planting pot 15 is opened, the other end of the ring cutter sampling tube is closed, the ring cutter sampling tube 12 is rotationally connected with the cylindrical shell 6, and the outer diameter of the ring cutter sampling tube 12 is smaller than the diameter of the second through hole 22;
The power piece 10 is arranged on the outer side wall of the cylindrical shell 6 and used for driving the ring cutter sampling tube 12 to rotate;
the auger 13 is rotatably arranged in the ring cutter sampling cylinder 12, and a second stepping motor 9 for driving the auger 13 to rotate is fixed at the other end of the ring cutter sampling cylinder 12 and can be fixed through screws;
The cylindrical shell 6 is internally provided with a cavity 30, the cavity 30 is obtained by rotary cutting of an isosceles trapezoid around the ring cutter sampling tube 12, the lower bottom of the isosceles trapezoid coincides with the outer wall of the ring cutter sampling tube 12, isosceles trapezoid rotating plates 28 matched with the cavity 30 are respectively fixed on two side walls of the ring cutter sampling tube 12 positioned in the cavity 30, the angle between the two rotating plates 28 is 180 degrees, and a soil outlet 29 is formed between the two rotating plates 28 on the wall of the ring cutter sampling tube 12.
When sampling is needed, the frame 20 is driven to rotate by the driving piece, the frame 20 drives the rubber sleeve 21 to rotate, the second through hole 22 is coincided with the first through hole 23, then according to the position to be sampled, the position of the sampling part is adjusted by rotating the first annular plate 4 and the second annular plate 5, the sampling angle of the annular cutter sampling cylinder 12 can be adjusted by the cooperation of different elongations of the first telescopic rod 7 and the second telescopic rod 11 and the rotation of the second annular plate 5, after the angle is adjusted, the annular cutter sampling cylinder 12 is driven to pass through the second through hole 22 and the first through hole 23 and is inserted into the planting pot 15, the annular cutter sampling cylinder 12 is driven to rotate by the power piece 10, soil and root systems are cut, the auger 13 is driven to rotate by the second stepping motor 9, the rotating direction of the auger 13 and the annular cutter sampling cylinder 12 is opposite, the auger 13 conveys soil and root systems in the soil to the soil outlet 29, the soil is crushed in the conveying process, the soil and root systems enter the cylindrical shell 6 from the soil outlet 29, the soil and the root systems are separated along with the rotation of the rotating plate 28, the first telescopic rod 7 and the second telescopic rod 11 are synchronously shortened after reaching the sampling depth, the open end of the ring cutter sampling cylinder 12 is driven to move to be close to the inner wall of the planting pot 15, then the shell door 27 is opened, the root systems in the soil can be picked out by forceps, of course, the soil and the root systems can be directly taken out of the cylindrical shell 6, the soil is put back into the inner cavity 30 of the cylindrical shell 6 through the shell door 27 after the root systems are picked, excessive restriction is not carried out, the auger 13 is driven to reversely rotate through the second stepping motor 9 after the root systems are sampled, meanwhile, the ring cutter sampling cylinder 12 is driven to rotate through the power piece 10, the ring cutter sampling cylinder 12 is driven to rotate through the rotating plate 28, through rotating plate 28 and cavity 30 lateral wall with soil direction outlet 29, can set up the rubber protrusion on cavity 30 lateral wall, at rotating plate 28 and rubber protrusion relative motion in-process, rotating plate 28 and cylinder casing 6 vibrations help soil to the outlet 29 motion, rethread auger 13 is sent back the soil to planting basin 15 in former sample position, the growth of alfalfa and continuous sampling operation of being convenient for, the soil returns the back, drive the cutting ring sampling tube 12 through first telescopic link 7 and second telescopic link 11 and remove to planting basin 15 outside, can carry out the sample of next position.
In this embodiment, all the stepper motors can rotate forward and backward, and can rotate forward and backward.
In summary, the invention provides a sampling device for saline-alkali soil alfalfa rhizosphere microorganisms, which has the following working principle:
Transplanting alfalfa seedlings with soil into the planting pot 15, and continuing to cultivate until plant roots spread on the planting pot 15;
the second annular plate 5 is driven to rotate on a vertical surface through a power structure, the second annular plate 5 drives the sampling component to move on the vertical surface, the first annular plate 4 is driven to rotate on a horizontal plane through the first stepping motor 3, the sampling component is driven to move on the horizontal plane, and the position of the sampling component is adjusted;
the sampling angle of the ring cutter sampling cylinder 12 is adjusted through the cooperation of different elongations of the first telescopic rod 7 and the second telescopic rod 11 and the rotation of the second ring plate 5;
The first telescopic rod 7 and the second telescopic rod 11 are synchronously extended, the ring cutter sampling cylinder 12 is driven to penetrate through the second through hole 22 and the first through hole 23 and is inserted into the planting pot 15, meanwhile, the power piece 10 is used for driving the ring cutter sampling cylinder 12 to rotate, soil and root systems are cut, meanwhile, the second stepping motor 9 is used for driving the auger 13 to rotate, the rotation direction of the auger 13 is opposite to that of the ring cutter sampling cylinder 12, the auger 13 is used for conveying soil and root systems in the soil to the soil outlet 29, the soil is crushed in the conveying process, and the soil and the root systems enter the cylindrical shell 6 from the soil outlet 29;
after reaching the sampling depth, the first telescopic rod 7 and the second telescopic rod 11 are synchronously shortened, the open end of the ring cutter sampling tube 12 is driven to move to be close to the inner wall of the planting pot 15, and then the shell door 27 is opened to pick out the root system in the soil;
The auger 13 is driven to reversely rotate through the second stepping motor 9, meanwhile, the power piece 10 drives the ring cutter sampling tube 12 to rotate, the ring cutter sampling tube 12 drives the rotating plate 28 to rotate, soil is guided to the soil outlet 29 through the rotating plate 28 and the side wall of the cavity 30, and the soil is returned to the original sampling position in the planting pot 15 through the auger 13.
It should be noted that, for the sake of simplicity of description, the foregoing embodiments are all described as a series of combinations of actions, but it should be understood by those skilled in the art that the present invention is not limited by the order of actions described, as some steps may be performed in other order or simultaneously in accordance with the present invention. Further, those skilled in the art will also appreciate that the embodiments described in the specification are all preferred embodiments, and that the acts and modules referred to are not necessarily required for the present invention.
The above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention. It will be apparent that the described embodiments are merely some, but not all, embodiments of the invention. Based on these embodiments, all other embodiments that may be obtained by one of ordinary skill in the art without inventive effort are within the scope of the invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art may still combine, add or delete features of the embodiments of the present invention or make other adjustments according to circumstances without any conflict, so as to obtain different technical solutions without substantially departing from the spirit of the present invention, which also falls within the scope of the present invention.
Claims (7)
1. A sampling device of saline-alkali soil alfalfa rhizosphere microorganism, characterized by comprising:
A frame;
A planting pot arranged on the frame, which is filled with saline-alkali soil for planting alfalfa;
The sampling unit is arranged on the frame and used for acquiring the root system of the alfalfa;
The middle positions of the tops of the two opposite side walls of the planting pot are respectively provided with a crankshaft in a rotating way, the crankshafts are in rotating connection with a rack, a fourth stepping motor for driving the crankshafts to rotate is fixed on the rack, and a plurality of first through holes are formed in the side walls and the bottom of the planting pot in a circumferential spacing way;
The sampling unit includes:
The first annular plate is arranged outside the planting pot and rotatably mounted on the frame, a first stepping motor for driving the first annular plate to rotate on a horizontal plane is fixed on the frame, vertical third telescopic rods are fixed at the bottoms of the front side wall and the rear side wall of the first annular plate, and supporting plates are fixed at the telescopic ends of the third telescopic rods and used for supporting the bottoms of the planting pot;
the second annular plate is coaxially and rotatably arranged on the first annular plate, and the first annular plate is provided with a power structure for driving the second annular plate to rotate on a vertical surface;
at least one sampling member mounted on the second ring plate;
the sampling member includes:
The cylinder shell is connected with the second annular plate through a first telescopic rod and a second telescopic rod, the first telescopic rod and the second telescopic rod are symmetrically arranged on the axis of the cylinder shell, one end of the first telescopic rod is fixedly connected with the second annular plate, the other end of the first telescopic rod is hinged with a sliding block, the sliding block is in sliding connection with the side wall of the cylinder shell, one end of the second telescopic rod is fixedly connected with the second annular plate, the other end of the second telescopic rod is hinged with the side wall of the cylinder shell, and a plurality of shell doors are arranged on the cylinder shell;
the ring cutter sampling tube is coaxially arranged with the cylindrical shell and penetrates through the cylindrical shell, the other end of the ring cutter sampling tube, which is close to the opening at one end of the planting pot, is closed, the ring cutter sampling tube is rotationally connected with the cylindrical shell, and the outer diameter of the ring cutter sampling tube is smaller than the diameter of the second through hole;
the power piece is arranged on the outer side wall of the cylindrical shell and used for driving the ring cutter sampling cylinder to rotate;
the auger is rotatably arranged in the ring cutter sampling cylinder, and a second stepping motor for driving the auger to rotate is fixed at the other end of the ring cutter sampling cylinder;
the cylindrical shell is internally provided with a cavity, the cavity is obtained by rotary cutting of an isosceles trapezoid around a ring cutter sampling cylinder, the lower bottom of the isosceles trapezoid coincides with the outer wall of the ring cutter sampling cylinder, isosceles trapezoid rotating plates matched with the cavity are respectively fixed on two side walls of the ring cutter sampling cylinder in the cavity, an angle between the two rotating plates is 180 degrees, and a soil outlet is formed on the cylinder wall of the ring cutter sampling cylinder between the two rotating plates;
The outside cover of planting the basin is equipped with the rubber sleeve, the rubber sleeve top is less than the top of planting the basin, the second through-hole has all been seted up in the rubber sleeve top that is located every first through-hole outside, the diameter of second through-hole is less than the diameter of first through-hole, and the interval of two adjacent first through-holes on the same circumference is greater than the diameter of second through-hole, the rubber sleeve outside is equipped with the frame, the frame top rotates with planting basin lateral wall to be connected, frame and rubber sleeve fixed connection, install the driving piece that is used for driving frame pivoted on the planting basin.
2. The sampling device for alfalfa rhizosphere microorganisms in saline-alkali soil according to claim 1, wherein the motive structure comprises:
the first gear is rotatably arranged on the first annular plate, and a fifth stepping motor for driving the first gear to rotate is fixed on the first annular plate;
And the first gear is fixed on the second annular plate and meshed with the first gear.
3. The sampling device for saline-alkali soil alfalfa rhizosphere microorganisms of claim 1, wherein the frame comprises:
a bottom plate;
The two side plates are symmetrically fixed on the bottom plate, and the crankshaft is rotationally connected with the side plates.
4. The sampling device for alfalfa rhizosphere microorganisms in saline-alkali soil according to claim 3, wherein supporting legs are symmetrically fixed on the lower portion of the first annular plate, and the supporting legs are slidably connected with annular sliding grooves formed in the upper surface of the bottom plate.
5. The sampling device of saline-alkali soil alfalfa rhizosphere microorganisms according to claim 1, wherein the frame comprises a plurality of connecting rings which are arranged at intervals from top to bottom, and a plurality of vertical connecting rods are fixedly connected between the connecting rings at intervals in the circumferential direction.
6. The sampling device for alfalfa rhizosphere microorganisms in saline-alkali soil according to claim 1, wherein the driver comprises:
a third stepping motor fixed at the top of the planting pot;
and a second gear fixed on the output shaft of the third stepping motor, and a second gear ring meshed with the second gear is fixed on the frame.
7. A sampling method based on the sampling device of saline-alkali soil alfalfa rhizosphere microorganisms according to any one of claims 1 to 6, characterized by comprising the following steps:
the second annular plate is driven to rotate on the vertical surface through the power structure, and the second annular plate drives the sampling component to move on the vertical surface;
the first annular plate is driven to rotate on the horizontal plane by the first stepping motor, so that the sampling component is driven to move on the horizontal plane;
After the position of the sampling part is adjusted, the sampling part enters the planting pot through the first through hole, and the root system is obtained.
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