CN117882742A - Carbon-based composite microorganism metabolic solution rice seed soaking agent and application thereof - Google Patents
Carbon-based composite microorganism metabolic solution rice seed soaking agent and application thereof Download PDFInfo
- Publication number
- CN117882742A CN117882742A CN202311085734.XA CN202311085734A CN117882742A CN 117882742 A CN117882742 A CN 117882742A CN 202311085734 A CN202311085734 A CN 202311085734A CN 117882742 A CN117882742 A CN 117882742A
- Authority
- CN
- China
- Prior art keywords
- seed soaking
- rice
- carbon
- soaking agent
- based composite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 141
- 235000009566 rice Nutrition 0.000 title claims abstract description 141
- 238000002791 soaking Methods 0.000 title claims abstract description 93
- 230000002503 metabolic effect Effects 0.000 title claims abstract description 75
- 239000002131 composite material Substances 0.000 title claims abstract description 42
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 37
- 244000005700 microbiome Species 0.000 title claims abstract description 30
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 141
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 69
- 230000012010 growth Effects 0.000 claims abstract description 42
- 230000035784 germination Effects 0.000 claims abstract description 39
- 241000223250 Metarhizium anisopliae Species 0.000 claims abstract description 31
- 230000000813 microbial effect Effects 0.000 claims abstract description 30
- 239000002207 metabolite Substances 0.000 claims abstract description 26
- 241000223259 Trichoderma Species 0.000 claims abstract description 21
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 19
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 13
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims abstract description 13
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims abstract description 13
- 150000001875 compounds Chemical class 0.000 claims abstract description 11
- 239000011734 sodium Substances 0.000 claims abstract description 8
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 5
- 241000223218 Fusarium Species 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 244000063299 Bacillus subtilis Species 0.000 claims description 14
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 13
- 241001460073 Trichoderma asperellum Species 0.000 claims description 9
- 230000002401 inhibitory effect Effects 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 9
- 239000000725 suspension Substances 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 230000003042 antagnostic effect Effects 0.000 claims description 5
- 238000011161 development Methods 0.000 claims description 5
- 230000018109 developmental process Effects 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 241000499912 Trichoderma reesei Species 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000010902 straw Substances 0.000 claims description 3
- 241000899289 Curvularia oryzae Species 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 244000061176 Nicotiana tabacum Species 0.000 claims description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 238000009331 sowing Methods 0.000 claims description 2
- 241000223211 Curvularia lunata Species 0.000 abstract description 5
- 241000813090 Rhizoctonia solani Species 0.000 abstract description 4
- 241001330975 Magnaporthe oryzae Species 0.000 abstract description 3
- 208000015181 infectious disease Diseases 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 62
- 230000000694 effects Effects 0.000 description 20
- 201000010099 disease Diseases 0.000 description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 16
- 230000005764 inhibitory process Effects 0.000 description 11
- 244000052616 bacterial pathogen Species 0.000 description 10
- 244000053095 fungal pathogen Species 0.000 description 8
- 239000008223 sterile water Substances 0.000 description 7
- 238000009629 microbiological culture Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 241000223195 Fusarium graminearum Species 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000002349 favourable effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 230000002786 root growth Effects 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000223208 Curvularia Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241001530056 Athelia rolfsii Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 230000002053 acidogenic effect Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 230000000069 prophylactic effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 241000294569 Aphelenchoides Species 0.000 description 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241000403356 Isoplia Species 0.000 description 1
- 241001470017 Laodelphax striatella Species 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 241000724205 Rice stripe tenuivirus Species 0.000 description 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000028446 budding cell bud growth Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229920003123 carboxymethyl cellulose sodium Polymers 0.000 description 1
- 229940063834 carboxymethylcellulose sodium Drugs 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000003170 nutritional factors Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000004476 plant protection product Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 230000021217 seedling development Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Toxicology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a carbon-based composite microorganism metabolic solution rice seed soaking agent and application thereof. The seed soaking agent comprises: complex microbial metabolites of trichoderma, metarhizium anisopliae and bacillus, biochar and sodium carboxymethyl cellulose (CMC-Na); wherein the content of the compound microorganism metabolic solution is 8-12% (v/v), the content of the biochar is 2-4% (w/v), and the content of sodium carboxymethyl cellulose (CMC-Na) is 0.3% -0.5% (v/v). The rice seed soaking agent can effectively promote the germination and growth of rice, and can effectively inhibit the growth and infection of Curvularia lunata, fusarium, rhizoctonia solani and Pyricularia oryzae.
Description
Technical Field
The invention relates to the technical field of rice seed soaking agents, in particular to a carbon-based composite microorganism metabolic solution rice seed soaking agent and application thereof.
Background
The germination rate of the rice seeds can be reduced by carrying fungi, and the diseases in the seedling stage, even the adult stage, are easily caused, so that huge losses are caused for the rice production. The rice seed soaking agent has important significance in promoting seedling growth, inhibiting early transmission of pathogenic bacteria, reducing pesticide consumption and ensuring green and safe rice production.
Seed treatment is mainly applied to biological, physical and chemical methods and means to provide protection for crop seeds so as to promote healthy emergence of crops. The seed treatment can promote the growth safety of crops and enable the crops to grow healthily, and compared with plant protection products acting on plant leaf surfaces, the seed treatment agent can control the occurrence of seed-borne diseases with very low dosage and higher control efficiency, so that the crops are prevented from being damaged by germs and underground pests, and is a primary link (Dungjiu building and the like, jiangsu agricultural science, 2017, 45 (15): 57-60) with important significance in the comprehensive treatment (IPM) of the crop diseases and insect pests. Seed treatment is a physical, chemical and biological method and means, and mainly comprises seed soaking, seed dressing, seed coating, seed pelleting and the like. The chemical agent seed soaking is an economic and effective method for preventing and controlling rice seed-borne and soil-borne diseases and seedling-stage diseases and insect pests, and can effectively prevent and prevent diseases such as bakanae disease, aphelenchoides disease and the like of rice and control the toxic transmission hazard of rice stripe virus-transmitted mediator Laodelphax striatellus at the initial stage of a seedling bed (Wen Tinggang and the like, jiangsu agricultural science, 2010 (2): 69-70). However, the continuous use of these seed soaking agents has resulted in problems of soil and water pollution and pathogen resistance in paddy fields, and has restricted green, ecological and sustainable production of paddy rice. Therefore, there is a need to create a novel microbial source seed soaking agent that promotes germination of rice seeds and seedling growth by metabolites of beneficial microorganisms, enhancing resistance of rice to seed-borne diseases and abiotic stress.
At present, only the institute of microbiology (2012) of the academy of sciences of the black dragon river in China has utilized a biological seed coating agent for preventing and treating bakanae disease of rice, which is composed of a bacillus amyloliquefaciens fermentation broth, a nutritional factor and a protective agent (scientific achievement, 2012). However, the biological seed soaking agent is a single viable bacteria preparation, is difficult to cope with infection of various bacteria carried by rice seeds, is difficult to compound with other agrochemicals, and has great popularization and application difficulty. The different application amounts of the rice straw biochar have obvious influence on the organic carbon and carbon nitrogen ratio and microbial diversity of rice field soil, and the biochar fertilizer plays a great role in the morphological establishment and physiological function enhancement of the root system of rice seedling stage (Liu Wei, heilongjiang agricultural science 2020 (11): 31-33).
In the prior art, no report has been made on the use of biochar-based materials as adsorption carriers for adsorbing microbial metabolic fluids and for treating crop seeds. The biochar has the functions of adsorbing and slowly releasing various nutrient substances and mineral substances due to the porosity and the larger specific surface area.
Therefore, the invention prepares the novel carbon and the composite microorganism metabolite seed soaking agent thereof by compounding or optimizing the metabolites of biocontrol microorganisms including Trichoderma asperellum (Trichoderma asperellum), bacillus subtilis (Bacillus subtilis) and metarhizium anisopliae (Metarhiziumannis isoplia), thereby realizing the source and system prevention and control of multiple rice seed-borne diseases and later-stage diseases and providing a new technology for green production of rice.
Disclosure of Invention
In view of the above, the technical problem to be solved by the invention is to provide a carbon-based composite microorganism metabolic solution rice seed soaking agent and application thereof. The rice seed soaking agent can effectively promote the germination and growth of rice, and can effectively inhibit the growth and infection of Curvularia lunata, fusarium, rhizoctonia solani and Pyricularia oryzae.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the first aspect of the invention provides a carbon-based composite microbial metabolizing fluid rice seed soaking agent, which comprises the following components:
complex microbial metabolites of trichoderma, metarhizium anisopliae and bacillus, biochar and sodium carboxymethyl cellulose (CMC-Na);
wherein the content of the compound microorganism metabolic solution is 8-12% (v/v), the content of the biochar is 2-4% (w/v), and the content of sodium carboxymethyl cellulose (CMC-Na) is 0.3% -0.5% (v/v).
Further, the content of the compound microorganism metabolic solution is 10% (v/v), the content of the biochar is 3% (w/v), and the content of sodium carboxymethyl cellulose (CMC-Na) is 0.3% (v/v).
Further, the preparation of the complex microbial metabolic solution comprises the following steps:
1) The concentration was 1X 10 6 Culturing the spore suspension of the Trichoderma reesei and the metarhizium anisopliae in PD medium at an inoculum size of 1% at 28 ℃ for 5d;
2) OD is set to 600 Bacillus=0.8 was diluted 100-fold and cultured in PD medium at 28 ℃ for 5d at an inoculum size of 1%;
3) Centrifuging the culture solution at 8000rpm/min for 10min, and filtering with sterile bacterial filter membrane to obtain sterile metabolic solution;
4) The aseptic metabolic solution of the trichoderma, the bacillus and the metarhizium anisopliae is prepared by the following components in volume ratio of 1:1:1, and obtaining the composite microorganism metabolic solution.
Further, the trichoderma is trichoderma asperellum (Trichoderma asperelum) 10264; the Metarrhizium anisopliae is Metarrhizium anisopliae (Metarhizium anisopliae) CGMCC 3.11962; the bacillus is bacillus subtilis (Bacillus subtilis) S4-4-10.
Further, the biochar is tobacco straw source biochar.
The second aspect of the invention provides application of the carbon-based composite microorganism metabolic solution rice seed soaking agent in inhibiting growth of Curvularia oryzae.
Furthermore, the application in inhibiting the growth of rice Curvularia is realized, and the inhibition rate of Curvularia reaches 83.15 +/-1.42%.
The third aspect of the invention provides an application of the carbon-based composite microorganism metabolic solution rice seed soaking agent in inhibiting growth of Fusarium oryzae.
Furthermore, the application in inhibiting the growth of rice Curvularia is realized, and the inhibition rate of Fusarium reaches 54.43 +/-1.33%.
The fourth aspect of the invention provides application of the carbon-based composite microorganism metabolic solution rice seed soaking agent in antagonizing rice sheath blight.
Further, the application of antagonizing rice sheath blight disease comprises: mixing the rice seed soaking agent with water according to the mass ratio of 1:10-12 to obtain seed soaking liquid; mixing the seed soaking liquid with rice seeds according to the proportion of 1.6L to 1.0-1.2kg, soaking the seeds at 28 ℃ for 48 hours, taking out the seeds, draining water, preserving moisture, accelerating germination for 5 days, and sowing.
Furthermore, when the flat plate antagonizes against rice sheath blight, the rice seed soaking agent can inhibit the growth of sheath blight germ hypha, and the inhibition rate is 60.95%.
The fifth aspect of the invention provides application of the carbon-based composite microorganism metabolic solution rice seed soaking agent in antagonizing rice blast.
Furthermore, the rice seed soaking agent can inhibit the growth of rice blast fungus hypha, and the inhibition rate is 30.01+/-2.47%.
The sixth aspect of the invention provides an application of the carbon-based composite microorganism metabolic solution rice seed soaking agent in improving the development and growth of rice seedlings.
Further, the application of the rice seedling development and growth improvement method comprises the following steps: mixing the rice seed soaking agent with water according to the mass ratio of 1:10-12 to obtain seed soaking liquid; the seed soaking liquid and rice seeds are mixed according to the proportion of 1.6L to 1.0-1.2kg, then the seeds are soaked for 48 hours at 28 ℃, the seeds are fished out, the moisture is drained, the moisture is preserved, the germination is accelerated for 5 days, and then the seeds are sown.
Furthermore, the application of the rice seed soaking agent in improving the development and growth of rice seedlings improves the bud growth promotion rate of the rice germination period by 26.9-42.1% and 25.1-26.9% compared with single metabolic solution and blank control.
The invention also provides application of the carbon-based composite microorganism metabolic solution rice seed soaking agent in improving rice tillering number.
Further, the application of the method for improving the tiller number of the rice comprises the following steps: mixing the rice seed soaking agent with water according to the mass ratio of 1:10-12 to obtain seed soaking liquid; the seed soaking liquid and rice seeds are mixed according to the proportion of 1.6L to 1.0-1.2kg, then the seeds are soaked for 48 hours at 28 ℃, the seeds are fished out, the moisture is drained, the moisture is preserved, the germination is accelerated for 5 days, and then the seeds are sown.
Furthermore, the rice seed soaking agent has growth promoting effect on the fresh weight and plant height of the rice in the 5-leaf stage and the tillering number, and the growth promoting rate is improved by 2.01% -9.27% and 9.85%, 1.80% -21.3% and 18.8%, 2.12% -20.1% and 17.2% compared with single metabolic solution and blank control respectively.
Trichoderma asperellum 10264 (Trichoderma asperelum), bacillus subtilis S4-4-10 (Bacillus subtilis) and metarhizium anisopliae (Metarhizium anisopliae) used in the invention are preserved in China general microbiological culture Collection center (CGMCC) No.22404, CGMCC No.27637 and CGMCC No.3.11962 respectively.
Wherein, the trichoderma asperellum 10264 (Trichoderma asperelum) is preserved in China general microbiological culture Collection center with the preservation number of CGMCC No.22404.
Specifically, the trichoderma asperellum 10264 (Trichoderma asperelum) has been preserved in the China general microbiological culture Collection center (CGMCC) of China general microbiological culture collection center (CGMCC) at the 4 th month 12 of 2021, and the preservation number is CGMCC No.22404.
Wherein, the bacillus subtilis S4-4-10 (Bacillus subtilis) is preserved in China general microbiological culture Collection center with the preservation number of CGMCC No.27637.
Specifically, the bacillus subtilis S4-4-10 (Bacillus subtilis) has been preserved in China general microbiological culture Collection center (CGMCC) for the year 6 and month 15 of 2023, and the preservation number is CGMCC No.27637.
Wherein the Metarhizium anisopliae (Metarhizium anisopliae) is commercially available.
As the rice seed-borne pathogenic bacteria have obvious influence on the germination and emergence of rice seeds and the occurrence of rice blast, bakanae disease and spike rot, how to control rice diseases from the source by inhibiting the seed-borne pathogenic bacteria is an important way.
Compared with the prior art, the invention has the following beneficial effects:
the carbon-based composite microorganism metabolic solution rice seed soaking agent of the invention does not pollute seeds, has no pesticide residue and no pollution to the paddy field environment, and is more beneficial to seed germination and cultivation of strong seedlings than chemical seed coating agents.
The carbon-based composite microbial metabolizing liquid rice seed soaking agent can induce more various rice seedling defense reaction or stress reaction related genes and growth related gene changes by three microbial metabolizing liquids, and has the effects of systematically inducing resistance, lasting disease prevention and growth promotion.
Drawings
FIG. 1 is a graph showing the plate inhibition of 4 rice pathogenic fungi by single and mixed metabolites;
FIG. 2 is a graph showing the control effect of single and composite seed soaking agents on rice sheath blight.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present invention, but should not be construed as limiting the invention. It should be noted that several modifications and improvements can be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present invention.
The cellular materials, reagents and laboratory apparatus used in the examples below are described below, and are conventional methods unless otherwise indicated.
T in the tables of the examples below represents a single Trichoderma asperellum metabolite, B represents a single Bacillus subtilis metabolite, M represents a single Metarhizium anisopliae metabolite, TMB represents a complex microbial metabolite, and CK represents a blank.
EXAMPLE 1 preparation of Compound microorganism-sterile Metabolic solution
1. Activating the flat-plate strain: trichoderma asperellum 10264 and Metarhizium anisopliae CGMCC 3.11962 are inoculated in a PDA culture dish, and bacillus subtilis S4-4-10 is inoculated in an LB culture dish for inverted culture for 4-5 days in a 28 ℃ incubator.
PDA medium: 200g of filtered upper juice obtained after potato is steamed, 20g of glucose, 20g of agar powder and purified water are added to a volume of 1L, and each 250mL of the juice is packaged into 500mL triangular flasks, and the juice is autoclaved at 121 ℃ for 25min.
LB solid medium: 10g of tryptone, 10g of NaCl, 5g of yeast extract, 20g of agar powder, purified water to 1L, naOH to adjust the pH to 7.4, subpackaging into 500mL triangular flasks every 250mL, and sterilizing at 121 ℃ for 25min.
2. Preparation of sterile metabolic solution: concentration is 1 x 10 6 Culturing the spore suspension of the Trichoderma reesei and the metarhizium anisopliae in PD medium at an inoculum size of 1% at 28 ℃ for 5d; OD is set to 600 Bacillus=0.8 was diluted 100-fold and cultured in PD medium at 28 ℃ for 5d at an inoculum size of 1%; centrifuging the culture solution at 8000rpm/min for 10min, and filtering with sterile bacterial filter membrane to obtain sterile metabolic solution.
Example 2 inhibition of Rice pathogenic fungi by Complex microbial Metabolic acid
1. Sterile metabolic solutions were prepared as in example 1.
2. Inhibition of rice pathogenic fungi: the single metabolic solution and the mixed metabolic solution are respectively mixed with PDA according to the following formula 1:3, inoculating rice pathogenic fungi at the center of the culture dish, taking pure PDA culture medium without metabolic solution as control, placing in a constant temperature incubator at 28deg.C for culturing, banded sclerotial blight culturing for 2-3d, and rice blast culturing for 7-10d. The diameter DT of the germ covered area with the complex metabolic solution and the diameter DR of the germ covered area cultivated by pure PDA are measured. The bacteriostasis rate for each treatment was calculated:
antibacterial ratio= (colony diameter of pathogenic bacteria in control plate-colony diameter of pathogenic bacteria covered with growth part treated with complex metabolite)/colony diameter of pathogenic bacteria in control plate x 100%.
3. The results show that: the single banded sclerotial blight and rice blast have the highest bacteriostasis rate of the metarhizium anisopliae, the single crescent moon spore has the highest bacteriostasis rate of the bacillus, and the single fusarium graminearum has the highest bacteriostasis rate of the metarhizium anisopliae and the bacillus; after being mixed according to the equal proportion, the inhibition rate of the rhizoctonia solani is more than 80 percent, and the inhibition rate of the rhizoctonia solani is more than 60 percent.
TABLE 1 inhibition effect of Compound microorganism Metabolic acid on pathogenic fungi of Rice
Example 3 Effect of mixing ratio of three microorganism Metabolic liquids on germination of Rice seeds
1. Sterile metabolic solutions were prepared as in example 1.
2. The mixing ratio of the three different microorganism metabolic liquids is listed as the influence on rice germination:
the trichoderma, the metarhizium anisopliae and the bacillus metabolic solution are mixed according to different proportions (2 ml), and the volume is fixed to 20ml by using sterile water to be used as a simple seed soaking agent. Sterilizing rice seeds with 75% alcohol for 5min, accelerating germination of 0.4g rice seeds in 20ml seed soaking agent for 2 days, taking out rice seeds, germinating every 30 rice seeds on a culture dish containing a certain amount of sterile water, counting rice germination condition every day, and measuring rice growth condition in 5d, wherein the root length and bud length of rice seedlings are used as indexes.
3. The results show that: trichoderma metabolic solution: metarrhizium anisopliae metabolic solution: bacillus metabolite=1:1:3, with best promoting effect on germination rate, sprout length and root length, the above ratio is 1:1: the growth of buds is most favored in the 1-hour, and the proportion is 1:2: root growth was most favored at 1 (Table 2).
Comprehensive antibacterial effect and dilution ratio, and optimal trichoderma metabolic solution: metarrhizium anisopliae metabolic solution: bacillus metabolite = 1:1:1. it is diluted 10 times, the germination rate is 91.11%, the root length is 2.98cm, and the bud length is 1.63cm; diluted 100 times, germination rate 86.67%, root length 2.87cm, bud length 1.07cm.
TABLE 2 influence of the mixing ratio of the three microorganism metabolites on the germination of rice seeds
Example 4 Effect of biochar and carboxymethylcellulose sodium salt on germination and growth of Rice seeds
1. Sterile metabolic solutions were prepared as in example 1.
2. Screening different biochar contents and carboxymethyl cellulose sodium salt contents: respectively weighing 0.2g, 0.4g, 0.6g and 0.8g of biochar, mixing with 2ml of metabolic solution, and then fixing the volume to 20ml by using sterile water;
2ml of the metabolite was diluted to 20ml, and sodium carboxymethyl cellulose was added in a total volume of 0.1%, 0.2%, 0.3%, 0.4%, respectively. The rice seeds are sterilized by using 75% alcohol for 5min,0.4g of the rice seeds are germinated in 20ml of seed soaking agent for two days, the rice seeds are fished out, every 30 rice seeds are placed on a culture dish containing a certain amount of sterile water for germination, the germination condition of the rice is counted every day, and the growth condition of the rice is measured in the 5 th day.
3. The results show that: when the metabolic solution is 2ml and the biochar is 0.6g, the germination rate is 85.56%, the root length is 5.55cm, and the bud length is 2.75cm, which is most favorable for the growth of the root and the bud. At a carboxymethylcellulose sodium salt content of 0.3%, the germination rate was 97.78%, the root length was 6.40cm, the bud length was 2.85cm, the germination rate was significantly higher than the rest of the treatment, and the growth of the root and bud was significantly promoted (Table 3).
TABLE 3 influence of biochar and carboxymethylcellulose sodium on germination and growth of rice seeds
Example 5 Effect of a metabolite seed soaking agent with biochar as an adsorption Carrier on germination and growth of Rice seeds
1. Sterile metabolic solutions were prepared as in example 1.
2. The biological carbon, the sodium carboxymethyl cellulose salt and the composite metabolic solution are screened out in the examples 2 and 3 and combined, and the importance degree of the biological carbon, the sodium carboxymethyl cellulose salt and the composite metabolic solution in the composite microorganism seed soaking agent is verified. Wherein: the biochar content is 0.6g, the carboxymethyl cellulose sodium salt is 0.3 percent of the total volume, the composite metabolic solution content is 2ml, and finally the mixture is mixed and then fixed to 20ml.
3. The results show that:
contains metabolic solution (2 ml), biochar (0.6 g) and sodium carboxymethyl cellulose (0.3%) which are obviously beneficial to the germination of rice and the growth of roots and buds. The presence of biochar favors the growth of shoots and sodium carboxymethyl cellulose favors the growth of roots (Table 4).
TABLE 4 Effect of metabolite seed soaking agent with biochar as adsorption Carrier on germination and growth of Rice seeds
Example 6 Effect of seed soaking agent on enzymatic Activity of Rice seedlings
1. Sterile metabolic solutions were prepared as in example 1.
2. 2ml of the composite metabolic solution is taken, diluted by 10 times, and then 0.6g of biochar and 0.3% of sodium carboxymethyl cellulose are added to obtain the seed soaking agent. The rice seeds are sterilized by using 75% alcohol for 5min,0.4g of the rice seeds are germinated in 20ml of seed soaking agent for two days, the rice seeds are fished out, and after germination for 5d, the rice with better germination is planted in a pot plant, and single metabolic solution and sterile water are used as a control. The change in PAL, SOD, CAT activity was measured. The enzyme activity difference was calculated using the fresh water treatment and PD medium as controls.
3. The results show that: the single seed soaking treatment of the trichoderma metabolizing solution induces the highest phenylalanine enzyme activity of rice, the highest catalase activity induced by the metarhizium anisopliae and the highest superoxide dismutase activity induced by the bacillus metabolizing solution. After the trichoderma metabolic solution, the metarhizium anisopliae metabolic solution, the bacillus metabolic solution and the like are mixed in proportion, the activity of the induced catalase and phenylalanine enzyme is highest, namely 3692.54 +/-1513.15U/g and 25.69+/-2.08U/g, which are obviously higher than that of single treatment; superoxide dismutase activity was also higher than for trichoderma and metarhizium anisopliae alone (table 5). In addition, the composite seed soaking agent effectively promotes the growth and development of rice, and the chlorophyll content and the nitrogen content of the composite seed soaking agent are obviously higher than those of single treatment and control; at the same time, the elevation of rice plants and the accumulation of biomass are promoted, and the tillering of rice is effectively promoted (Table 6).
TABLE 5 Effect of seed soaking agents on enzymatic Activity of Rice seedlings
TABLE 6 Effect of seed soaking agents on rice growth
EXAMPLE 7 seed soaking agent controlling effect on sheath blight
1. Sterile metabolic solutions were prepared as in example 1.
2. 2ml of the composite metabolic solution is taken, diluted by 10 times, and then 0.6g of biochar and 0.3% of sodium carboxymethyl cellulose are added to obtain the seed soaking agent. The rice seeds are sterilized by using 75% alcohol for 5min,0.4g of the rice seeds are germinated in 20ml of seed soaking agent for two days, the rice seeds are fished out, and after germination for 5d, the rice with better germination is planted in a pot plant, and single metabolic solution and sterile water are used as a control.
3. Mixing wheat grains containing sheath blight bacteria with soil according to the proportion of 3% -5%, transplanting rice seedlings for 5d by utilizing single carbon-based metabolic solution and carbon-based composite metabolic solution and biochar soaking seed for germination acceleration, and measuring the growth amount, plant height and overground part weight of the rice after a period of growth. And (3) taking the soil without disease as a control, and measuring the inhibition of the carbon-containing microbial metabolite rice seed soaking agent on rice sheath blight.
4. Experimental design for inhibiting Curvularia lunata and Fusarium graminearum
Preventive group: after 24 hours of seed soaking of the grains with the carbon-based complex metabolic solution, the grains are respectively soaked in the suspensions of the crescent spore and the fusarium graminearum (10) 6 cfu/ml) for 24 hours.
Treatment group: immersing in a suspension of Curvularia lunata and Fusarium graminearum (10) 6 cfu/ml) 24 hours, the seeds were immersed in sterile water as a control at the 24-hour period of the seed-immersed charcoal-based complex metabolite.
30 rice seeds are taken and placed on a 9cm culture dish, three repetitions are arranged for each treatment, moisture preservation and germination acceleration are carried out, and the germination rate, root length and bud length of the seeds are counted.
5. The results show that: the sheath blight can be effectively prevented and treated by seed soaking, and the effect of the compound seed soaking agent is optimal and reaches 76.56 +/-1.59 (Table 7). When seeds are soaked singly, the metabolic solution of the trichoderma and the metarhizium anisopliae is beneficial to the development of rice; the Trichoderma and Bacillus metabolites were more favorable for rice embryo and root development (tables 8-10).
For root growth: the preventive treatment (seed soaking first and then seed soaking spore suspension of pathogenic bacteria) was more favorable to root growth than the therapeutic treatment (seed soaking spore suspension of pathogenic bacteria and then seed soaking).
For the growth of buds: depending on the pathogenic bacteria, the trend is different, and the treatment with Curvularia lunata appears to be a prophylactic group in favor of embryo development.
In contrast, the treatment with fusarium graminearum appears to be: the treatment group was more favorable to germ growth (table 10). Regardless of the prophylactic or therapeutic group, when the trichoderma metabolic solution, the metarhizium anisopliae metabolic solution, the bacillus metabolic solution, etc. are mixed in proportions (TMB), the germination rate, root length and bud length are generally significantly higher than those of the single metabolic solution and control.
TABLE 7 control effect of seed soaking agent on sheath blight
TABLE 8 influence of different interaction modes of Complex microorganism Metabolic acid and pathogenic fungi on Rice germination Rate
TABLE 9 Effect of different interaction modes of Complex microbial Metabolic fluids and pathogenic fungi on Rice root growth
TABLE 10 influence of different interaction patterns of Complex microbial Metabolic acid and pathogenic fungi on Rice bud Length
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (14)
1. A carbon-based composite microbial metabolite rice seed soaking agent, the seed soaking agent comprising:
complex microbial metabolites of trichoderma, metarhizium anisopliae and bacillus, biochar and sodium carboxymethyl cellulose (CMC-Na);
wherein, the culture medium prepared by the compound microorganism metabolic solution is PD culture medium: 6g/L potato extract powder, 20g/L glucose. Wherein the content of the compound microorganism metabolic solution is 8-12% (v/v), the content of the biochar is 2-4% (w/v), and the content of sodium carboxymethyl cellulose (CMC-Na) is 0.3% -0.5% (v/v).
2. The carbon-based composite microbial metabolizing fluid rice seed soaking agent of claim 1, wherein the carbon-based composite microbial metabolizing fluid rice seed soaking agent is characterized by: the content of the compound microorganism metabolic liquid is 10% (v/v), the content of the biochar is 3% (w/v), and the content of sodium carboxymethyl cellulose (CMC-Na) is 0.3% (v/v).
3. The carbon-based composite microbial metabolizing fluid rice seed soaking agent of claim 1, wherein the carbon-based composite microbial metabolizing fluid rice seed soaking agent is characterized by: the preparation of the compound microorganism metabolic solution comprises the following steps:
1) The concentration was 1X 10 6 Culturing the spore suspension of the Trichoderma reesei and the metarhizium anisopliae in PD medium at an inoculum size of 1% at 28 ℃ for 5d;
2) OD is set to 600 Bacillus=0.8 was diluted 100-fold and cultured in PD medium at 28 ℃ for 5d at an inoculum size of 1%;
3) Centrifuging the culture solution at 8000rpm/min for 10min, and filtering with sterile bacterial filter membrane to obtain sterile metabolic solution;
4) The aseptic metabolic solution of the trichoderma, the bacillus and the metarhizium anisopliae is prepared by the following components in volume ratio of 1:1:1, and obtaining the composite microorganism metabolic solution.
4. The carbon-based composite microbial metabolizing fluid rice seed soaking agent of claim 1, wherein the carbon-based composite microbial metabolizing fluid rice seed soaking agent is characterized by: the trichoderma is trichoderma asperellum (Trichoderma asperelum) 10264; the Metarrhizium anisopliae is Metarrhizium anisopliae (Metarhizium anisopliae) CGMCC 3.11962; the bacillus is bacillus subtilis (Bacillus subtilis) S4-4-10.
5. The carbon-based composite microbial metabolizing fluid rice seed soaking agent of claim 1, wherein the carbon-based composite microbial metabolizing fluid rice seed soaking agent is characterized by: the biochar is tobacco straw source biochar.
6. Use of a carbon-based composite microbial metabolite rice seed soaking agent according to any one of claims 1-4 for inhibiting growth of Curvularia oryzae.
7. Use of a carbon-based composite microbial metabolite rice seed soaking agent according to any one of claims 1-4 for inhibiting growth of fusarium oryzae.
8. The use of a carbon-based composite microbial metabolite rice seed soaking agent according to any one of claims 1-4 for antagonizing rice sheath blight.
9. The use according to claim 7, characterized in that: the application comprises: mixing the rice seed soaking agent with water according to the mass ratio of 1:10-12 to obtain seed soaking liquid; mixing the seed soaking liquid with rice seeds according to the proportion of 1.6L to 1.0-1.2kg, soaking the seeds at 28 ℃ for 48 hours, taking out the seeds, draining water, preserving moisture, accelerating germination for 5 days, and sowing.
10. The use of a carbon-based composite microbial metabolite rice seed soaking agent according to any one of claims 1-4 for antagonizing rice blast.
11. Use of a carbon-based composite microbial metabolizing fluid rice seed soaking agent according to any one of claims 1-4 for improving the development and growth of rice seedlings.
12. The use according to claim 10, characterized in that: the application comprises: mixing the rice seed soaking agent with water according to the mass ratio of 1:10-12 to obtain seed soaking liquid; the seed soaking liquid and rice seeds are mixed according to the proportion of 1.6L to 1.0-1.2kg, then the seeds are soaked for 48 hours at 28 ℃, the seeds are fished out, the moisture is drained, the moisture is preserved, the germination is accelerated for 5 days, and then the seeds are sown.
13. Use of a carbon-based composite microbial metabolizing fluid rice seed soaking agent according to any one of claims 1-4 for increasing tiller number of rice.
14. The use according to claim 12, characterized in that: the application comprises: mixing the rice seed soaking agent with water according to the mass ratio of 1:10-12 to obtain seed soaking liquid; the seed soaking liquid and rice seeds are mixed according to the proportion of 1.6L to 1.0-1.2kg, then the seeds are soaked for 48 hours at 28 ℃, the seeds are fished out, the moisture is drained, the moisture is preserved, the germination is accelerated for 5 days, and then the seeds are sown.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311085734.XA CN117882742A (en) | 2023-08-25 | 2023-08-25 | Carbon-based composite microorganism metabolic solution rice seed soaking agent and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311085734.XA CN117882742A (en) | 2023-08-25 | 2023-08-25 | Carbon-based composite microorganism metabolic solution rice seed soaking agent and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117882742A true CN117882742A (en) | 2024-04-16 |
Family
ID=90644770
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311085734.XA Pending CN117882742A (en) | 2023-08-25 | 2023-08-25 | Carbon-based composite microorganism metabolic solution rice seed soaking agent and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117882742A (en) |
-
2023
- 2023-08-25 CN CN202311085734.XA patent/CN117882742A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101473853B (en) | Application of Bacillus cereus CMCC63305 in agricultural field | |
| CN111172080A (en) | Bacillus belgii and application thereof | |
| CN109022320B (en) | Siamese bacillus and application thereof in preventing and treating root rot and southern blight | |
| Maheshwari et al. | Co-inoculation of urea and DAP tolerant Sinorhizobium meliloti and Pseudomonas aeruginosa as integrated approach for growth enhancement of Brassica juncea | |
| CN112940991B (en) | Antibacterial activity of Siamese bacillus and application thereof | |
| CN112746024A (en) | Trichoderma composite microbial agent and bio-organic fertilizer prepared from same | |
| CN115997791A (en) | Application of Burkholderia cepacia in promoting saline-alkali tolerance of plants and reducing heavy metal cadmium content | |
| CN113755408B (en) | Multifunctional bacillus subtilis, composite microbial inoculum and application thereof | |
| CN102465096A (en) | Microbial agent as well as preparation method and application thereof | |
| CN106701635B (en) | Banana endophytic streptomycete with root-knot nematode killing capability and biological seedling culture substrate developed by banana endophytic streptomycete | |
| CN116590155B (en) | Paenispira rosea strain, microbial agent, preparation method and application | |
| CN110129242B (en) | Continuous cropping resistant composite microbial preparation and preparation method thereof | |
| CN106396836B (en) | Soybean phytophthora root rot prevents and treats biological seed coating agent and preparation method thereof, application method | |
| CN113293112B (en) | Composite microbial soil remediation agent and preparation method thereof | |
| CN111517863B (en) | Bio-organic fertilizer containing bacillus methylotrophicus and preparation method thereof | |
| CN117882742A (en) | Carbon-based composite microorganism metabolic solution rice seed soaking agent and application thereof | |
| CN114149935A (en) | Lysobacter enzymogenes and application thereof | |
| CN112831430A (en) | Comamonas aquosus strain, biocontrol microbial inoculum and preparation method and application thereof | |
| CN111758742A (en) | Microbial pesticide synergist suitable for wheat and application thereof | |
| CN117887589B (en) | Trichoderma FJ059 capable of parasitizing sclerotium of southern blight and application thereof | |
| LU502648B1 (en) | Trichoderma-based seed coating agent, preparation method and application thereof | |
| CN113068716B (en) | Compound agent for promoting growth of potato seedlings and application thereof | |
| CN111019837B (en) | New application of trichoderma viride | |
| CN117821331B (en) | Composite microbial agent and preparation method and application thereof | |
| CN117511925A (en) | Microbial agent, preparation method and application thereof, and method for preventing and controlling crop pathogenic bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |