CN117866755A - Quick-temperature-changing heat accumulating type PCR instrument - Google Patents
Quick-temperature-changing heat accumulating type PCR instrument Download PDFInfo
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- CN117866755A CN117866755A CN202310316096.1A CN202310316096A CN117866755A CN 117866755 A CN117866755 A CN 117866755A CN 202310316096 A CN202310316096 A CN 202310316096A CN 117866755 A CN117866755 A CN 117866755A
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- 238000005338 heat storage Methods 0.000 claims abstract description 120
- 238000010438 heat treatment Methods 0.000 claims abstract description 40
- 230000008859 change Effects 0.000 claims abstract description 16
- 230000005540 biological transmission Effects 0.000 claims description 24
- 238000001514 detection method Methods 0.000 claims description 11
- 229910001369 Brass Inorganic materials 0.000 claims description 3
- 239000010951 brass Substances 0.000 claims description 3
- 239000007769 metal material Substances 0.000 claims description 2
- 238000009825 accumulation Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 238000001816 cooling Methods 0.000 description 4
- 230000000630 rising effect Effects 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/14—Thermal energy storage
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Abstract
The invention discloses a rapid temperature-changing heat accumulating type PCR instrument, which comprises a heated chip and a heating component, wherein the heating component comprises a first heat accumulating element and a second heat accumulating element, the temperature of the first heat accumulating element is lower than that of the heated chip, and the temperature of the second heat accumulating element 5 is higher than that of the heated chip; and the first heat storage element and the second heat storage element are respectively arranged below and above the heated chip in a vertically sliding way. When the device is used, the second heat storage element and the first heat storage element are brought into a state required for changing temperature in advance, and when the temperature needs to be raised or lowered, the second heat storage element or the first heat storage element is attached to the heated chip, so that the second heat storage element or the first heat storage element and the heated chip are fully thermally convected, the heated chip is further raised or lowered to a target temperature, the temperature change of a sample in the heated chip can be controlled more accurately in the whole process, the specificity of the PCR reaction is improved, the temperature change is realized more quickly, and the speed of the PCR reaction is improved.
Description
Technical Field
The invention belongs to the field of medical instruments, and particularly relates to a rapid temperature-changing thermal storage type PCR instrument.
Background
The polymerase chain reaction (Polymerase Chain Reaction, PCR) is a molecular biological reaction for DNA amplification. One of the key factors for success or failure of the temperature-variable PCR reaction is that the temperature cycle conditions required by the reaction entity, namely high temperature denaturation, low temperature annealing and intermediate temperature primer extension, must be satisfied, so that the temperature cycle is the basis, and the "temperature-variable" rate in the temperature cycle determines the specificity of the PCR reaction and the overall speed of the PCR operation. The conventional PCR thermal cycle system is largely classified into a water bath type, an air flow type, and a semiconductor type, and the heating method thereof is to heat a test tube by transferring heat of a heat generating source to a heating device through heat transfer by heating a resistance wire, a resistance sheet, or a semiconductor combined heat cover, etc. The temperature rising rate is 2-3 ℃/s, and 30-cycle experiments are completed once, which takes 2-3 hours.
In large scale applications where special conditions are in demand, many laboratories place high demands on the speed of detection. Meanwhile, each manufacturer and scientific research institution improve the temperature rising speed and the temperature reducing speed in PCR. The main types are two, one is to increase power and to perform temperature changing operation by means of higher energy output. Such as high power peltier, high power heating sheet and high power fan combinations, etc.; the other is to improve the efficiency and transfer the energy to the reagent more accurately by various novel heating modes. Such as infrared heating, electromagnetic heating, silicon-based consumable heating, and the like.
Technical disadvantages of the prior art: the disadvantage of the first scheme is that the number of heat transfer stages is large, the conversion efficiency is low, the utilization rate is poor, and meanwhile, the whole PCR instrument is large in machine, high in price, high in energy consumption and the like due to the intermediate equipment. The second solution has the disadvantage of high cost and complex structure of infrared heating, electromagnetic heating or silicon-based consumable heating.
Therefore, there is an urgent need for a PCR instrument with a small number of heat transfer stages, high utilization rate, low cost, and simple structure.
Disclosure of Invention
The invention aims to solve the problems of multiple heat transfer stages, low conversion efficiency, poor utilization rate, high price, high energy consumption or complex structure of the conventional PCR instrument when a sample is heated.
The technical scheme adopted by the invention is as follows:
a rapid temperature-changing thermal storage type PCR instrument comprises an instrument main body, a heated chip, an instrument cover body and a heating component;
the heating assembly comprises a first heat storage element and a second heat storage element, the temperature of the first heat storage element is lower than that of the heated chip, and the temperature of the second heat storage element is higher than that of the heated chip;
the first heat storage element and the second heat storage element are respectively movably arranged in the instrument main body and the instrument cover body;
the heated chip is arranged between the instrument main body and the instrument cover body;
when the heated chip needs to be heated, the second heat storage element is close to and in contact with the upper surface of the heated chip, the first heat storage element is far away from the heated chip, and the temperature of the heated chip after the temperature rising target temperature is equal to the temperature of the second heat storage element and the heated chip after full heat convection;
when the heated chip needs to be cooled, the second heat storage element is close to and in contact with the lower surface of the heated chip, the second heat storage element is far away from the heated chip, and the temperature of the cooled target of the heated chip is equal to the temperature of the first heat storage element and the heated chip after full heat convection.
Optionally, one or more sample bins are arranged on the heated chip, and the first heat storage element and the second heat storage element are respectively positioned right below and right above the sample bins.
Optionally, the first heat storage element, the second heat storage element and the sample bin are all made of metal materials.
Optionally, the first heat storage element, the second heat storage element and the sample bin are made of brass.
Alternatively, the first heating electrode is disposed on both sides of the first heat storage element, and the second heating electrode is disposed on both sides of the second heat storage element.
Optionally, a second driving motor is arranged on the instrument cover body, and a transmission gear is arranged on a power output shaft of the second driving motor;
one side of the second driving motor is vertically provided with a transmission rack, and the transmission rack is meshed with the transmission gear;
a position detection switch is arranged on one side of the transmission rack, which is far away from the second driving motor;
the bottom of the transmission rack is provided with a second heat storage element, and the top of the transmission rack is provided with an in-place detection sheet.
Optionally, a lifting device and a first driving motor are arranged in the inner cavity of the instrument main body; the movable end of the lifting device is provided with a first heat storage element;
the power output shaft of the first driving motor is connected with the heated chip;
one side at the top of the instrument main body is provided with a hinged connecting piece, and the instrument main body is connected with the instrument cover body through the hinged connecting piece.
Alternatively, the heating assembly replaces the first heat storage element and the second heat storage element with a plurality of circumferential heat storage elements surrounding the outer side of the heated chip, the plurality of circumferential heat storage elements have the same temperature, and the target temperature for changing the temperature of the heated chip is equal to the temperature of the heated chip and the circumferential heat storage elements after sufficient heat convection.
The beneficial effects of the invention are as follows:
the invention provides a rapid temperature-changing regenerative PCR instrument, which comprises an instrument main body, a heated chip, an instrument cover body and a heating component, wherein the heating chip is arranged on the instrument main body; the heating assembly comprises a first heat storage element and a second heat storage element, the temperature of the first heat storage element is lower than that of the heated chip, and the temperature of the second heat storage element is higher than that of the heated chip; the first heat storage element and the second heat storage element are respectively movably arranged in the instrument main body and the instrument cover body; the heated chip is mounted between the instrument body and the instrument cover.
1) When the temperature-changing device is used, the second heat storage element and the first heat storage element are brought into a state required for changing temperature in advance by calculating the energy required by the temperature-changing or temperature-reducing of the heated chip, and when the temperature-changing or temperature-reducing is required, the second heat storage element or the first heat storage element is attached to the heated chip, so that the second heat storage element or the first heat storage element and the heated chip are fully thermally convected, the heated chip is further heated or cooled to the target temperature, the temperature change of a sample in the heated chip can be controlled more accurately in the whole process, the specificity of the PCR reaction is improved, the temperature change is realized more rapidly, and the speed of the PCR reaction is improved.
2) Compared with the heating or cooling mode of the PCR instrument in the current market, the heating speed and the cooling speed of the invention are obviously accelerated; meanwhile, the first heat storage element and the second heat storage element have no complex structure, and the heat storage device is low in manufacturing cost and durable.
3) Compared with the heating or cooling mode of the PCR instrument in the current market, the first heat storage element and the second heat storage element only have two actions of attaching and leaving, and the control is simple.
Drawings
Fig. 1 is a schematic structural view of the present invention.
FIG. 2 is a schematic view of the structure of a PCR instrument equipped with the present invention.
FIG. 3 is a schematic diagram of a structure in which the PCR instrument of the present invention is mounted and a heated chip has been omitted.
Fig. 4 is a schematic view of the internal structure of the instrument cover.
Fig. 5 is a schematic diagram of the connection relationship of the second heat storage element.
Fig. 6 is a schematic view of the structure of the instrument body lumen.
Fig. 7 is a schematic diagram of the structure of a heated chip.
Fig. 8 is a schematic diagram of a structure in which the circumferential heat storage element is separated from the heated chip in the second embodiment.
Fig. 9 is a schematic structural diagram of the circumferential heat storage element and the heated chip bonded in the second embodiment.
In the figure: the device comprises a 1-instrument main body, a 11-lifting device, a 12-first driving motor, a 13-hinged connector, a 2-first heat storage element, a 21-first heating electrode, a 3-heated chip, a 31-sample bin, a 4-instrument cover body, a 41-second driving motor, a 42-transmission gear, a 43-transmission rack, a 44-in-place detection sheet, a 45-position detection switch, a 5-second heat storage element and a 51-second heating electrode.
Detailed Description
Embodiment one:
in this embodiment, as shown in fig. 1, a rapid temperature-changing thermal storage PCR apparatus includes a heated chip 3 and a heating assembly, the heating assembly includes a first thermal storage element 2 and a second thermal storage element 5, the temperature of the first thermal storage element 2 is lower than that of the heated chip 3, and the temperature of the second thermal storage element 5 is higher than that of the heated chip 3; and the first heat storage element 2 and the second heat storage element 5 are slidably mounted below and above the heated chip 3, respectively.
When the heated chip 3 needs to be heated, the second heat storage element 5 is close to and in contact with the upper surface of the heated chip 3, the first heat storage element 2 is far away from the heated chip, and the temperature of the heated chip 3 at the temperature of the heated chip is equal to the temperature of the second heat storage element 5 and the heated chip 3 after full heat convection; when the heated chip 3 needs to be cooled, the second heat storage element 5 is close to and in contact with the lower surface of the heated chip 3, the second heat storage element 5 is far away from the heated chip, and the temperature of the cooled target of the heated chip 3 is equal to the temperature of the first heat storage element 2 and the heated chip 3 after sufficient heat convection. The whole process of heating or cooling the heated chip 3 to the target temperature can be more rapid and accurate, the temperature change of the sample environment in the heated chip 3 can be controlled more accurately, the sample environment temperature in the heated chip 3 can be controlled to a specific temperature required by high-temperature denaturation, low-temperature annealing or medium-temperature primer extension accurately, the specificity of temperature circulation in the PCR reaction is improved, the temperature change is realized more rapidly, and the speed of the PCR reaction is improved.
In this embodiment, as shown in fig. 2 and 3, the present invention further includes an instrument main body 1 and an instrument cover 4. The first heat storage element 2 and the second heat storage element 5 are respectively movably arranged in the instrument main body 1 and the instrument cover body 4; the heated chip 3 is mounted between the instrument main body 1 and the instrument cover 4, and the first heat storage element 2 and the second heat storage element 5 are located directly below and directly above the sample chamber 31, respectively, when the instrument cover 4 is covered on the instrument main body 1.
In this embodiment, the first heat storage element 2, the second heat storage element 5 and the sample chamber 31 may be made of metal or other materials with high heat conduction efficiency and low cost, for example, brass materials may be used for the first heat storage element 2, the second heat storage element 5 and the sample chamber 31.
In the present embodiment, as shown in fig. 1 and 5, the first heat storage element 2 is provided with the first heating electrode 21 on both sides, the second heat storage element 5 is provided with the second heating electrode 51 on both sides, and the first heating electrode 21 and the second heating electrode 51 are respectively connected to a power supply, thereby realizing the heating of the first heat storage element 21 and the second heat storage element 51 to a temperature required for the temperature change of the heated chip 3 in advance.
In the present embodiment, as shown in fig. 4 and 5, a second drive motor 41 is provided on the instrument cover 4, and a transmission gear 42 is provided on the power output shaft of the second drive motor 41; a transmission rack 43 is vertically arranged on one side of the second driving motor 41, and the transmission rack is meshed with the transmission gear 42; a position detection switch 45 is arranged on one side of the transmission rack 43 away from the second driving motor 41; the second heat storage element 5 is installed at the bottom end of the transmission rack 43, and the in-place detection sheet 44 is installed at the top end of the transmission rack 43. The second driving motor 41 drives the driving gear 42 to rotate, so as to drive the transmission rack 43 to move up and down, and further, the transmission rack 43 can drive the second heat storage element 5 to be closely attached to or far away from the upper surface of the heated chip 3.
In the present embodiment, as shown in fig. 5, a U-shaped mounting block is provided on one side of the drive rack 43, the opening of the U-shaped mounting block faces the in-place detecting piece 44, two position detecting switches 45 are provided in the vertical direction in the U-shaped mounting block, and when the position detecting switch 45 above the inside of the U-shaped mounting block detects the in-place detecting piece 44, the second drive motor 41 stops rotating, and the second heat storage element 5 stops rising; when the transmission rack 43 moves downwards to the position detection switch 45 below the U-shaped installation block and the in-place detection sheet 44 is not detected, the second driving motor 41 stops rotating, the second heat storage element 5 stops descending, and the second heat storage element 5 is attached to the sample bin 31 on the upper surface of the heated chip 3.
In this embodiment, as shown in fig. 6, a lifting device 11 and a first driving motor 12 are installed in the inner cavity of the instrument main body 1; the movable end of the lifting device 11 is provided with a first heat storage element 2; the power output shaft of the first driving motor 12 is connected with the heated chip 3; one side at the top of the instrument main body 1 is provided with a hinged connecting piece 13, the instrument main body 1 is connected with the instrument cover body 4 through the hinged connecting piece 13, and the first driving motor 12 can drive the heated chip 3 to rotate. The lifting device 11 is used for driving the first heat storage element 2 to ascend or descend, and the lifting device 11 is a reciprocating cylinder or an electric push rod.
In this embodiment, as shown in fig. 7, one or more sample bins 31 are disposed on the heated chip 3, and the specific calculation formula is as follows by calculating the energy required to be absorbed or released by all the sample bins 31 on the heated chip 3 to change to the target temperature:
Q＝cmΔT；
wherein Q is the amount of heat absorbed (or evolved);
m is the mass of the object;
Δt is the amount of change in endothermic (or exothermic) temperature;
c is the specific heat capacity of the material;
the mass and the initial temperature of the heated chip 3 are both known or measurable, and the specific heat capacity c of the heated chip material can be queried according to the related data, so that the temperature change Δt of the heated chip 3 from the temperature to the target temperature can be obtained, and the energy Q required for the heated chip 3 to change to the target temperature can be obtained according to the above formula under the condition that the above parameters are known.
Meanwhile, the current temperature, mass m and specific heat c of the first heat storage element 2 and the second heat storage element 5 can be measured or inquired; according to the above formula, the second heat storage element 5 can be heated to a certain temperature higher than the target temperature of the heated chip 3, and the second heat storage element 5 is higher than the target temperature of the heated chip 3, and the energy of the second heat storage element is the same as the energy required by the heated chip 3 to change to the target temperature, so that after the second heat storage element 5 and the heated chip 3 are fully thermally convected, the second heat storage element 5 is cooled to the target temperature after the heated chip 3 is heated; the same principle can make the temperature of the first heat storage element 2 lower than a certain temperature of the target temperature of the heated chip 3, and the energy required to be absorbed by the first heat storage element 2 when the temperature of the first heat storage element 2 is raised to the target temperature of the heated chip 3 is the same as the energy required to be emitted by the heated chip 3 when the temperature of the first heat storage element is lowered to the target temperature, so that after the first heat storage element 2 and the heated chip 3 are subjected to heat convection, the first heat storage element 2 and the heated chip 3 are both changed to the same temperature, namely the target temperature of the heated chip 3.
Embodiment two:
the difference between this embodiment and the first embodiment is that:
in the present embodiment, as shown in fig. 8 and 9, the heating assembly replaces the first heat storage element 2 and the second heat storage element 5 with a plurality of circumferential heat storage elements surrounding the outside of the heated chip 3, the plurality of circumferential heat storage elements have the same temperature, and the target temperature at which the heated chip 3 is changed in temperature is equal to the temperature at which the heated chip 3 and the circumferential heat storage elements are sufficiently thermally convected.
When the temperature of the circumferential heat storage elements is higher than that of the heated chip 3, after the plurality of circumferential heat storage elements are simultaneously attached to the heated chip 3, the temperature of the heated chip 3 is raised; when the temperature of the circumferential heat storage elements is lower than that of the heated chip 3, the plurality of circumferential heat storage elements are simultaneously attached to the heated chip 3, and then the temperature of the heated chip 3 is reduced.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.
Claims (8)
1. A heat accumulation formula PCR appearance of quick alternating temperature, its characterized in that: comprises an instrument main body (1), a heated chip (3), an instrument cover body (4) and a heating component;
the heating assembly comprises a first heat storage element (2) and a second heat storage element (5), wherein the temperature of the first heat storage element (2) is lower than that of the heated chip (3), and the temperature of the second heat storage element (5) is higher than that of the heated chip (3);
the first heat storage element (2) and the second heat storage element (5) are respectively movably arranged in the instrument main body (1) and the instrument cover body (4);
the heated chip (3) is arranged between the instrument main body (1) and the instrument cover body (4);
when the heated chip (3) needs to be heated, the second heat storage element (5) is close to and in contact with the upper surface of the heated chip (3), the first heat storage element (2) is far away from the heated chip, and the temperature of the heated chip (3) is equal to the temperature of the second heat storage element (5) and the heated chip (3) after sufficient heat convection;
when the heated chip (3) needs to be cooled, the second heat storage element (5) is close to and in contact with the lower surface of the heated chip (3), the second heat storage element (5) is far away from the heated chip, and the temperature of the cooled target of the heated chip (3) is equal to the temperature of the first heat storage element (2) and the heated chip (3) after sufficient heat convection.
2. The rapid temperature-changing thermal storage type PCR instrument according to claim 1, wherein one or more sample bins (31) are arranged on the heated chip (3), and the first thermal storage element (2) and the second thermal storage element (5) are respectively positioned right below and right above the sample bins (31).
3. The rapid temperature change thermal storage type PCR instrument as claimed in claim 2, wherein the first thermal storage element (2), the second thermal storage element (5) and the sample bin (31) are made of metal materials.
4. A rapid temperature change thermal storage PCR instrument according to claim 3, wherein the first thermal storage element (2), the second thermal storage element (5) and the sample compartment (31) are all made of brass.
5. The rapid temperature-changing thermal storage type PCR instrument according to claim 1, wherein the first heating electrode (21) is arranged on both sides of the first thermal storage element (2), and the second heating electrode (51) is arranged on both sides of the second thermal storage element (5).
6. The rapid temperature-changing thermal storage type PCR instrument according to claim 1, wherein a second driving motor (41) is arranged on the instrument cover body (4), and a transmission gear (42) is arranged on a power output shaft of the second driving motor (41);
a transmission rack (43) is vertically arranged on one side of the second driving motor (41), and the transmission rack is meshed with the transmission gear (42);
a position detection switch (45) is arranged on one side of the transmission rack (43) away from the second driving motor (41);
the bottom end of the transmission rack (43) is provided with a second heat storage element (5), and the top end of the transmission rack (43) is provided with an in-place detection sheet (44).
7. The rapid temperature-changing thermal storage type PCR instrument according to claim 1, wherein a lifting device (11) and a first driving motor (12) are arranged in the inner cavity of the instrument main body (1); the movable end of the lifting device (11) is provided with a first heat storage element (2);
the power output shaft of the first driving motor (12) is connected with the heated chip (3);
one side at the top of the instrument main body (1) is provided with a hinged connecting piece (13), and the instrument main body (1) is connected with the instrument cover body (4) through the hinged connecting piece (13).
8. The rapid temperature change thermal storage type PCR instrument according to claim 1, wherein the heating assembly replaces the first thermal storage element (2) and the second thermal storage element (5) by a plurality of circumferential thermal storage elements which surround the outer side of the heated chip (3), the plurality of circumferential thermal storage elements have the same temperature, and the target temperature of the temperature change of the heated chip (3) is equal to the temperature after the heated chip (3) and the circumferential thermal storage elements are fully thermally convected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310316096.1A CN117866755A (en) | 2023-03-24 | 2023-03-24 | Quick-temperature-changing heat accumulating type PCR instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310316096.1A CN117866755A (en) | 2023-03-24 | 2023-03-24 | Quick-temperature-changing heat accumulating type PCR instrument |
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| CN117866755A true CN117866755A (en) | 2024-04-12 |
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|---|---|---|---|
| CN202310316096.1A Pending CN117866755A (en) | 2023-03-24 | 2023-03-24 | Quick-temperature-changing heat accumulating type PCR instrument |
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| CN (1) | CN117866755A (en) |
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- 2023-03-24 CN CN202310316096.1A patent/CN117866755A/en active Pending
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