CN117866038A - Method for purifying affinity tagged acidic proteins containing host nucleic acids - Google Patents
Method for purifying affinity tagged acidic proteins containing host nucleic acids Download PDFInfo
- Publication number
- CN117866038A CN117866038A CN202410271265.9A CN202410271265A CN117866038A CN 117866038 A CN117866038 A CN 117866038A CN 202410271265 A CN202410271265 A CN 202410271265A CN 117866038 A CN117866038 A CN 117866038A
- Authority
- CN
- China
- Prior art keywords
- pei
- affinity
- column
- acidic protein
- rinsing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 101800000263 Acidic protein Proteins 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 30
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 29
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 27
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 37
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 37
- 239000000243 solution Substances 0.000 claims abstract description 27
- 238000001042 affinity chromatography Methods 0.000 claims abstract description 20
- 238000011068 loading method Methods 0.000 claims abstract description 14
- 150000003839 salts Chemical class 0.000 claims abstract description 9
- 239000012460 protein solution Substances 0.000 claims abstract description 7
- 239000003480 eluent Substances 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 125000002091 cationic group Chemical group 0.000 claims description 3
- 239000003446 ligand Substances 0.000 claims description 3
- 125000000129 anionic group Chemical group 0.000 claims description 2
- 150000001450 anions Chemical class 0.000 abstract description 2
- 150000001768 cations Chemical class 0.000 abstract description 2
- 238000011067 equilibration Methods 0.000 abstract 1
- 229920002873 Polyethylenimine Polymers 0.000 description 56
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
- 238000004255 ion exchange chromatography Methods 0.000 description 14
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 10
- 239000007983 Tris buffer Substances 0.000 description 9
- 150000002500 ions Chemical class 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 239000011780 sodium chloride Substances 0.000 description 9
- 239000002253 acid Substances 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 230000006820 DNA synthesis Effects 0.000 description 2
- 238000005571 anion exchange chromatography Methods 0.000 description 2
- 238000005277 cation exchange chromatography Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 108091006055 affinity-tagged proteins Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229940126601 medicinal product Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
The present invention provides a method for purifying an affinity tagged acidic protein comprising a host nucleic acid, the method comprising loading an affinity tagged acidic protein solution comprising a host nucleic acid onto an affinity chromatography column, rinsing the affinity chromatography column with an equilibration solution; rinsing the affinity chromatographic column with PEI solution, and purifying with cation chromatographic column or anion chromatographic column after the eluted protein is replaced to low salt system. According to the invention, one step of PEI rinsing is added in the original affinity chromatography step, and the HCD residue problem is effectively solved by optimizing the PEI rinsing concentration and volume.
Description
Technical Field
The present invention relates to the field of protein purification, and in particular to a method for purifying an affinity tagged acidic protein containing a host nucleic acid.
Background
The recombinant protein product is widely applied to the fields of scientific research, biochemical analysis or medical treatment, a large amount of Host nucleic acid (HCD) residues are associated in the process of extracting and purifying the recombinant protein, particularly in molecular experiments, the Host nucleic acid can interfere with the analysis and judgment of experimental results, and the residual HCD in the biological medicinal product can bring cancerogenic and infection risks, so that the purified recombinant protein with low HCD residues has important significance.
To achieve high purity, low residual affinity tagged proteins, the first purification is usually affinity chromatography to enrich the protein; the second step of ion chromatography improves purity and reduces impurity residues. However, the acid protein with high requirement for HCD residue (the acid protein described in the patent refers to the protein with negative total surface net charge in the purified solution), the HCD content is difficult to be effectively reduced to below 100pg/mg after affinity-ion chromatography, and the ion chromatography method cannot effectively separate the acid protein from the HCD because the acid protein and the HCD surface are negatively charged, which greatly increases the purification difficulty and the process cost of the acid protein.
PEI (Polyethylenimine) is a molecular polymer with higher water solubility, is also currently known cationic organic high molecular material with the largest charge density, and has the advantages of high adhesiveness, high adsorptivity, high cationicity, high reactivity, low cytotoxicity and the like. In the industry, a PEI precipitation method is generally adopted to remove HCD, but the method has the problems of large protein loss, incomplete nucleic acid removal and PEI residue on acidic proteins. Thus, new techniques are needed to solve the HCD residue problem of proteins.
Disclosure of Invention
In order to solve the above technical problems, the present invention provides a method for purifying an affinity tagged acidic protein containing a host nucleic acid, the method comprising the steps of: step 1: loading an affinity tagged acidic protein solution containing host nucleic acids onto an affinity chromatography column, wherein the acidic protein specifically binds to ligands on the affinity chromatography column; step 2: rinsing the affinity chromatographic column with an equilibrium solution after loading is completed; step 3: after step 2, rinsing the affinity chromatography column with PEI solution, wherein PEI is washed with nucleic acid in the rinsing process, and the acidic protein is still bound on the affinity chromatography column; step 4: rinsing the affinity chromatographic column with an equilibrium solution, and eluting the acidic protein from the affinity chromatographic column with an eluent after rinsing; and, step 5: and after the eluted protein is replaced to a low-salt system, purifying by using a cationic chromatographic column, eluting the acidic protein with low salt, or purifying by using an anionic chromatographic column, removing PEI possibly remained with low salt, eluting the acidic protein with high salt, and separating the acidic protein and PEI according to the difference between the PEI and the acidic protein on the ion column, thereby obtaining the purified acidic protein.
In one embodiment, the PEI rinse volume in step 2 is not less than 5 volumes and PEI volume concentration is not less than 0.003%.
In one embodiment, the PEI rinse volume in step 2 is no greater than 9 column volumes and the PEI volume concentration is no greater than 0.004%.
In one embodiment, the PEI rinse volume in step 2 is 8 column volumes and the PEI volume concentration is 0.004%.
In one embodiment, the acidic protein is a molecular enzyme.
In the present invention, after the protein solution is on the affinity column, the protein is specifically bound with the affinity ligand on the chromatography column to form a ligand-protein complex; for certain specific proteins such as molecular enzymes (molecular enzymes in this application refer to enzymes whose substrates are nucleic acids and which can bind to nucleic acids), ligand-molecular enzyme-nucleic acid complexes are formed, from which nucleic acids are difficult to separate by conventional purification means, but the method of the invention can perfectly solve the separation problem. When PEI rinses the affinity column, the PEI has positive charges with high density, the nucleic acid is negatively charged, the PEI wraps the nucleic acid in the affinity column, the nucleic acid is forcedly peeled off from the protein to form PEI-nucleic acid complex, and the ionic acting force between molecules is generally smaller than the affinity, so that the protein is still combined on the chromatographic column in the rinsing process, and the nucleic acid is wrapped and eluted by the PEI.
When PEI rinses the affinity column, PEI forms PEI-nucleic acid complex by coating nucleic acid in the affinity column due to PEI with cations and nucleic acid with anions, and the PEI is rinsed with nucleic acid in the rinsing process and protein is still bound on the chromatography column due to the fact that intermolecular ionic forces are generally smaller than affinities.
The acidic protein solution eluted from the affinity column may have PEI residue, and under low salt conditions the acidic protein may flow through the cation chromatography column or the anion chromatography column, but under low salt conditions the PEI may flow through the anion chromatography column or the cation chromatography column, and the acidic protein and PEI may be separated according to the difference between the PEI and the acidic protein on the ion column.
The method has the advantages that the process is simple, the HCD in the obtained acidic protein is thoroughly removed, the HCD removal rate is more than 99%, the minimum HCD content can be reduced to 10pg/mg, and the protein loss is less than 10%. The method solves the problems of relatively complex process, overlarge protein loss and higher nucleic acid residue of the existing acid protein with the affinity tag in the HCD removal technology.
According to the invention, one step of PEI rinsing is added in the original affinity chromatography step, and the HCD residue problem is effectively solved by optimizing the PEI rinsing concentration and volume.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a contour plot of HCD residual versus PEI concentration, rinse volume in the present invention;
FIG. 2 is a contour plot of relative loss of protein versus PEI concentration, rinse volume for the present invention.
Detailed Description
In order that those skilled in the art will better understand the technical solutions in the present application, the present invention will be further described with reference to examples. It will be apparent that the described embodiments are only some, but not all, of the embodiments of the present application. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments herein without making any inventive effort, shall fall within the scope of the present application. In the following examples, unless otherwise indicated, all methods conventional in the art are described.
Experimental protocol
1. Breaking bacteria to obtain clarified protein solution;
2. loading and rinsing by affinity chromatography: respectively loading the protein solution on the 16 affinity columns, and rinsing residual supernatant with a balancing solution after loading;
3. PEI rinsing affinity chromatography column: sorting the 16 affinity columns, see table 1, and rinsing the affinity columns with the corresponding PEI rinse solution, respectively; rinsing residual PEI with an affinity chromatography equilibrium solution again;
4. eluting the target protein from the affinity column: eluting proteins on the 16 affinity chromatographic columns by using an affinity chromatographic eluent;
5. loading and rinsing an ion column: respectively diluting the eluted proteins by using ion chromatography balance liquid; loading the diluted proteins into 16 ion columns respectively, and rinsing the ion columns with balance liquid after loading;
6. eluting the target protein from the ion column: eluting target proteins from the ion column by using ion chromatography eluent, and detecting protein concentration and host nucleic acid residues of the eluted proteins.
Second, materials
Materials: 16 affinity chromatography high-flux purification gun heads with the same specification and 16 ion chromatography high-flux purification gun heads with the same specification;
the bacterial cells: a bacterial cell expressed by the His-tagged acidic protein BESN0024 in Escherichia coli (BL 21 (DE 3));
PEI: polyethylenimine, wobbe, [9002-98-6], M.W.70000.
Tris: tris (hydroxymethyl) aminomethane, hu test, lot: 20230726
NaCl: sodium chloride, hu test, lot: 20230915
Imidazole: meicolin, lot: C14739942
Comma biological DNA synthesis was performed using a sieve plate Φ4.1mm thick with a 2.5mm pore size of 20. Mu.m.
1mL pipette tip without filter element.
Affinity filler: NW Rose NiFF, nami, lot: L04D0701
Ion chromatography packing: SP Sepharose High Performance, situo vacuum, lot: 10333816.
Third, purifying the concrete steps
1. Reagent preparation: the affinity chromatography phase uses reagents: affinity chromatography equilibrium solution A is 20mM Tris,500mM NaCl,25mM imidazole, and the pH value is 8.0; PEI rinse A1 solution 20mM Tris,500mM NaCl,25mM imidazole, pH8.0+0.001% PEI; PEI rinse A2 solution 20mM Tris,500mM NaCl,25mM imidazole, pH8.0+0.005% PEI; PEI rinse A3 solution 20mM Tris,500mM NaCl,25mM imidazole, pH8.0+0.01% PEI; PEI rinse A4 solution, 20mM Tris,500mM NaCl,25mM imidazole, pH8.0+0.02% PEI; affinity chromatography eluent B, 20mM Tris,500mM NaCl,250mM imidazole, pH8.0. Ion chromatography uses reagents: ion chromatography balance liquid SPA liquid: 20mM Tris, pH8.0; ion chromatography eluent SPB liquid: 20mM Tris,100mM NaCl,pH8.0.
High-flux purification gun head preparation: taking 1mL gun head, adding a piece of screen plate for DNA synthesis as a lower plug, compacting the plug, adding 500 mu L of Ni chromatographic packing solution, and sub-packaging 16 affinity chromatographic gun head marks of 0/a1/b1/c1/a2/b2/c2/a3/b3/c3/a4/b4/c4/a5/b5/c5; and assembling 16 ion chromatography gun heads in the same method, and marking SP 1-16 respectively.
2.5 g of BESN0024 bacterial cells were added to 50mL of the affinity column A solution, and the mixture was subjected to ultrasonic disruption for 20 minutes after resuspension. After the bacterial suspension was centrifuged at 12000rpm for 30 minutes, the supernatant was filtered through a 0.22 μm filter.
3. The upper ends of 16 gun heads marked as 0/a1/b1/c1/a2/b2/c2/a3/b3/c4/a4/b4/c4/a5/b5/c5 are respectively balanced by 5mL of balance liquid affinity column A, 2mL of bacterial breaking supernatant is loaded after the balance, and 2.5mL of rinsing by the affinity column A is completed after the loading.
4. The gun head of a1/b1/c1 was rinsed with solution A in amounts of 2.5mL, 5mL and 7.5mL, respectively. The a2/b2/c2 gun head was rinsed with solution A1 in 2.5mL, 5mL, and 7.5mL, respectively. The a3/b3/c3 gun head was rinsed with 2.5mL, 5mL, and 7.5mL of A2 solution, respectively. The a4/b4/c4 gun head was rinsed with 2.5mL, 5mL and 7.5mL of A3 solution, respectively. The a5/b5/c5 gun head was rinsed with 2.5mL, 5mL, and 7.5mL of A4 solution, respectively. 2.5mL of the affinity column A solution was added to the tips of the a1/b1/c1/a2/b2/c2/a3/b3/c3/a4/b4/c4/a5/b5/c5 guns, respectively, for rinsing.
5. After rinsing, 1mL of eluent is added into a 0/a1/b1/c1/a2/b2/c2/a3/b3/c3/a4/b4/c4/a5/b5/c5 gun head, target proteins are eluted, and eluting components are respectively collected and marked as Ni-1/2/3/4/5/6/7/8/9/10/11/12/13/14/15/16.
6. And diluting Ni-1-16 with 9mL of ion chromatography balance liquid SPA respectively. And (3) respectively loading the diluted Ni-1-16 proteins to corresponding SP 1-16 ion chromatographic columns, and rinsing 2.5mL by using balance liquid SPA liquid after loading.
7. And after the ion column rinsing is finished, respectively adding 1mL of ion chromatography eluent SPB into the SP 1-16 ion chromatography columns, eluting the target protein, and respectively collecting elution components and marking as 1/2/3/4/5/6/7/8/9/10/11/12/13/14/15/16. The concentrations of the 1-16 component proteins and the residual host nucleic acids were measured, and the measurement results are shown in Table 1 below.
TABLE 1 results of different PEI volume concentrations and rinse volumes on protein loss and host nucleic acid residues
。
The data of Table 1 were processed to obtain a contour plot of FIG. 1, HCD residual versus PEI concentration, rinse volume, using PEI concentration and rinse volume as factors and host nucleic acid residual as response values. As can be seen from FIG. 1, the HCD residual is less than 100pg/mg when the PEI rinse volume is not less than 5 column volumes and the PEI volume concentration is not less than 0.003%.
The contour plot of the relative protein loss rate versus PEI concentration, rinse volume was obtained in FIG. 2 in response to the relative protein loss rate taking PEI concentration and rinse volume as factors. As can be seen from fig. 2, the relative protein loss rate was below 10% when the PEI rinse volume was no greater than 9 column volumes and the PEI volume concentration was no greater than 0.004%.
Conclusion: after the first step of Ni enrichment, it can be seen from fig. 1 and 2 that the best HCD removal is achieved when the PEI rinse volume is between 5 and 9 column volumes and the PEI volume concentration is between 0.003% and 0.004%, and the optimum PEI volume concentration is selected comprehensively to be 0.004% and the rinse volume is 8 column volumes.
It is to be understood that this invention is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.
Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are also encompassed by the appended claims.
Claims (5)
1. A method for purifying an affinity tagged acidic protein comprising a host nucleic acid, said method comprising the steps of:
step 1: loading an affinity tagged acidic protein solution containing host nucleic acids onto an affinity chromatography column, wherein the acidic protein specifically binds to ligands on the affinity chromatography column;
step 2: rinsing the affinity chromatographic column with an equilibrium solution after loading is completed;
step 3: after step 2, rinsing the affinity chromatography column with PEI solution, wherein PEI is washed with nucleic acid in the rinsing process, and the acidic protein is still bound on the affinity chromatography column;
step 4: rinsing the affinity chromatographic column with an equilibrium solution, and eluting the acidic protein from the affinity chromatographic column with an eluent after rinsing;
step 5: and after the eluted protein is replaced to a low-salt system, purifying by using a cationic chromatographic column, eluting the acidic protein with low salt, or purifying by using an anionic chromatographic column, removing PEI possibly remained with low salt, eluting the acidic protein with high salt, and separating the acidic protein and PEI according to the difference between the PEI and the acidic protein on the ion column, thereby obtaining the purified acidic protein.
2. The method of claim 1 wherein the PEI rinse volume in step 2 is not less than 5 volumes and the PEI volume concentration is not less than 0.003%.
3. The method of claim 2 wherein the PEI rinse volume in step 2 is no greater than 9 column volumes and the PEI volume concentration is no greater than 0.004%.
4. A method according to claim 3, wherein the PEI rinse volume in step 2 is 8 column volumes and the PEI volume concentration is 0.004%.
5. The method of any one of claims 1-4, wherein the acidic protein is a molecular enzyme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410271265.9A CN117866038A (en) | 2024-03-11 | 2024-03-11 | Method for purifying affinity tagged acidic proteins containing host nucleic acids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410271265.9A CN117866038A (en) | 2024-03-11 | 2024-03-11 | Method for purifying affinity tagged acidic proteins containing host nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117866038A true CN117866038A (en) | 2024-04-12 |
Family
ID=90581592
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410271265.9A Pending CN117866038A (en) | 2024-03-11 | 2024-03-11 | Method for purifying affinity tagged acidic proteins containing host nucleic acids |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117866038A (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104125963A (en) * | 2011-12-22 | 2014-10-29 | 弗·哈夫曼-拉罗切有限公司 | Ion exchange membrane chromatography |
| US20160287693A1 (en) * | 2013-11-15 | 2016-10-06 | Novartis Ag | Removal of residual cell culture impurities |
| CN110387363A (en) * | 2019-08-28 | 2019-10-29 | 莫纳(武汉)生物科技有限公司 | A kind of Restriction enzyme Sma I and its expression and purification method |
| US20200317727A1 (en) * | 2017-12-19 | 2020-10-08 | CSL Behring Lengnau AG | Protein purification and virus inactivation with alkyl glycosides |
| CN114316066A (en) * | 2021-11-11 | 2022-04-12 | 元本(珠海横琴)生物科技有限公司 | MNR2 protein purification method |
| CN114369640A (en) * | 2021-03-08 | 2022-04-19 | 南京诺唯赞生物科技股份有限公司 | Method for quickly and efficiently removing animal-derived host nucleic acid |
| CN115925890A (en) * | 2021-09-28 | 2023-04-07 | 江苏泰康生物医药有限公司 | Method for purifying anti-new coronavirus neutralizing antibody |
| CN116234619A (en) * | 2020-08-18 | 2023-06-06 | 赛多利斯比亚分离有限责任公司 | Metal affinity extraction of host cell DNA |
| US20230242939A1 (en) * | 2020-01-29 | 2023-08-03 | Voyager Therapeutics, Inc. | Methods and systems for producing aav particles |
| CN116694584A (en) * | 2023-02-27 | 2023-09-05 | 武汉瀚海新酶生物科技有限公司 | Preparation method of T4DNA ligase |
| CN117230039A (en) * | 2023-07-20 | 2023-12-15 | 武汉瀚海新酶生物科技有限公司 | Inorganic pyrophosphatase separation and purification method |
-
2024
- 2024-03-11 CN CN202410271265.9A patent/CN117866038A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104125963A (en) * | 2011-12-22 | 2014-10-29 | 弗·哈夫曼-拉罗切有限公司 | Ion exchange membrane chromatography |
| US20160287693A1 (en) * | 2013-11-15 | 2016-10-06 | Novartis Ag | Removal of residual cell culture impurities |
| US20200317727A1 (en) * | 2017-12-19 | 2020-10-08 | CSL Behring Lengnau AG | Protein purification and virus inactivation with alkyl glycosides |
| CN110387363A (en) * | 2019-08-28 | 2019-10-29 | 莫纳(武汉)生物科技有限公司 | A kind of Restriction enzyme Sma I and its expression and purification method |
| US20230242939A1 (en) * | 2020-01-29 | 2023-08-03 | Voyager Therapeutics, Inc. | Methods and systems for producing aav particles |
| CN116234619A (en) * | 2020-08-18 | 2023-06-06 | 赛多利斯比亚分离有限责任公司 | Metal affinity extraction of host cell DNA |
| CN114369640A (en) * | 2021-03-08 | 2022-04-19 | 南京诺唯赞生物科技股份有限公司 | Method for quickly and efficiently removing animal-derived host nucleic acid |
| CN115925890A (en) * | 2021-09-28 | 2023-04-07 | 江苏泰康生物医药有限公司 | Method for purifying anti-new coronavirus neutralizing antibody |
| CN114316066A (en) * | 2021-11-11 | 2022-04-12 | 元本(珠海横琴)生物科技有限公司 | MNR2 protein purification method |
| CN116694584A (en) * | 2023-02-27 | 2023-09-05 | 武汉瀚海新酶生物科技有限公司 | Preparation method of T4DNA ligase |
| CN117230039A (en) * | 2023-07-20 | 2023-12-15 | 武汉瀚海新酶生物科技有限公司 | Inorganic pyrophosphatase separation and purification method |
Non-Patent Citations (4)
| Title |
|---|
| JUNFEN MA等: "Using precipitation by polyamines as an alternative to chromatographic separation in antibody purification processes", 《JOURNAL OF CHROMATOGRAPHY B》, vol. 878, 4 February 2010 (2010-02-04), pages 798 - 806, XP026933296, DOI: 10.1016/j.jchromb.2010.01.044 * |
| RUI NIAN等: "Polyethyleneimine-Mediated Chemical Extraction of Cytoplasmic His-Tagged Inclusion Body Proteins from Escherichia coli", 《BIOTECHNOLOGY PROGRESS》, vol. 24, 24 January 2008 (2008-01-24), pages 417 - 425 * |
| 叶福强等: "宏基因组测序去除宿主核酸方法的研究进展", 《中国国境卫生检疫杂志》, 10 March 2024 (2024-03-10), pages 1 - 7 * |
| 徐梦蔚等: "实验室水平单克隆抗体制备方法及相应纯化策略的研究进展", 《中国生物制品学杂志》, vol. 35, no. 02, 20 February 2022 (2022-02-20), pages 228 - 232 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HRP20180182T1 (en) | Correctly folded etanercept in high purity and excellent yield | |
| JP6456376B2 (en) | Purification method of recombinant protein | |
| WO2007075283A2 (en) | Polishing steps used in multi-step protein purification processes | |
| US20180148712A1 (en) | Method and Test Kit For Rapid Isolation of Nucleic Acids Using Rough Surfaces | |
| EP3663401B1 (en) | Purification process for biological molecules such as plasmid dna using anionic exchange chromatography | |
| US9458452B2 (en) | Method and device for isolating and purifying double-stranded nucleic acids | |
| JP7111373B2 (en) | Amphoteric dissociation type ion exchange media, method of use and calibration of separation capacity | |
| CN117866038A (en) | Method for purifying affinity tagged acidic proteins containing host nucleic acids | |
| EP1232006A1 (en) | A method for selective removal of a substance from samples containing compounds having nucleic acid structure | |
| Harkensee et al. | Fast screening for the purification of proteins using membrane adsorber technology | |
| US20230151351A1 (en) | A method of single-stranded rna purification | |
| CN116261483A (en) | Multimode metal affinity processing capsid | |
| US20110152510A1 (en) | Simple load and elute process for purification of genomic dna | |
| CN114249793A (en) | Recombinant protein purification method | |
| US20070256976A1 (en) | Metal-coated sorbents as a separation medium for HPLC of phosphorus-containing materials | |
| WO2011060438A2 (en) | Protein separation via ion-exchange chromatography and associated methods, systems and devices | |
| WO2021044029A1 (en) | Compositions and methods for reducing chromatin content of biological preparations | |
| US8653239B2 (en) | Method for isolating polypeptides | |
| CN113388609B (en) | Purification method of large plasmid DNA | |
| EP3995196B1 (en) | Purification process for lipid-membrane-enclosed complex assemblages | |
| US20230142600A1 (en) | A method of single-stranded rna purification | |
| CN115058414A (en) | Method for purifying plasmid DNA | |
| CN116609447A (en) | Liquid chromatography analysis method for simultaneously detecting multiple proteins | |
| Wan et al. | Historical Perspectives | |
| EP4225462A1 (en) | Improved removal of rna and contaminants from dna plasmid preparations by hydrophobic interaction chromatography |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination |