CN117859769A - Salt-tolerant bacillus and biological organic fertilizer and application thereof - Google Patents
Salt-tolerant bacillus and biological organic fertilizer and application thereof Download PDFInfo
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Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to salt-tolerant bacillus and a biological organic fertilizer and application thereof, wherein the preservation number is as follows: cctccc NO: salt-tolerant bacillus of M20232276Bacillus halotolerans) The biological organic fertilizer containing salt-tolerant bacillus, mushroom fungus chaff, vetch straw, gibberellic acid and bamboo leaf extract can be used for preventing and treating crop root rot and improving organic carbon content of plant rhizosphere soil,The nitrogen absorption and utilization rate of plants is improved, and the proline content of root systems of the plants is improved. The salt-tolerant bacillus is a natural strain, is easier to colonize in soil, does not have adverse effect on environmental safety, and can promote sustainable development of agricultural production.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to salt-tolerant bacillus and a biological organic fertilizer and application thereof.
Background
In recent years, with the continuous year planting of crops with high added value and the development of protected areas, soil-borne diseases are aggravated year by year, and if the soil-borne diseases are not intervened in time, the crops are seriously ill-received. Especially fungal diseases in soil-borne diseases, can cause serious effects on plant growth, such as cell rot, leaf apoptosis, inflorescence deformation, etc., and even death. However, in disease control, when a single, especially targeted agent is used for controlling soil-borne diseases, not only is an ideal control effect difficult to achieve, but also the pathogen can generate drug resistance.
Over the last 10 years, with the attention of people to the environment, green development has become the mainstream of agricultural production. In the prevention and treatment of soil-borne diseases, the influence on the environment and ecology is reduced as much as possible, and better economic benefit is ensured. Compared with chemical pesticides, biological pesticide control is to inhibit the growth of pathogens by utilizing microbial preparations and plant preparations, and gradually becomes the current hot research field. The microbial preparation can promote the microbial diversity in soil, increase the soil fertility and the crop quality, and improve the immunity and the resistance of plants. In addition, the biological organic fertilizer contains living microorganisms, so that the growth of crops can be promoted. Compared with chemical fertilizers, the biological organic fertilizer has the characteristics of high carbon nitrogen ratio and comprehensive fertilizer efficiency, has better improvement effect on physical and chemical properties of soil, can improve the microbial activity of the soil, and improve the soil fertility and the crop yield and quality, and has important significance in environmental protection and promotion of soil material circulation.
In addition, the bio-organic fertilizer used at present usually adopts mutant or directionally modified strains, and the strains are often limited by environmental factors in practical application, so that the strains are difficult to colonize, grow and play a proper role in soil or plants. Thus, natural strain-based research into bio-organic fertilizers is becoming a hotspot in the art.
In summary, for biological prevention and control of crop diseases, it is necessary to reduce the influence on the environment and ecology as much as possible, and to ensure better economic benefits, and the bio-organic fertilizer is an emerging fertilizer type, and needs to be further researched and applied on the premise of being based on natural strains, so as to improve the preparation technology and application effect thereof.
Disclosure of Invention
In order to overcome the problems in the background technology, the invention provides salt-tolerant bacillus and a biological organic fertilizer and application thereof. Compared with genetically engineered bacteria, the salt-tolerant bacillus provided by the invention has better soil and plant rhizosphere colonization capacity, and the microbial inoculum and the bio-organic fertilizer containing the bacillus have the advantages of preventing and treating crop root rot, improving the proline content of plant root system, improving the root system activity and the like after being applied.
To achieve the above object, the present invention provides a storage number of: cctccc NO: salt-tolerant bacillus of M20232276Bacillus halotolerans) The application of the plant growth regulator in preventing and treating crop root rot, improving the organic carbon content of plant rhizosphere soil, improving the nitrogen absorption and utilization rate of plants and improving the proline content of plant root systems.
The invention also provides a bio-organic fertilizer, which comprises the following components:
component A: comprises the following components in percentage by weight: 4-6:1-5:0.5-1 of mushroom bran, vetch straw, gibberellic acid and bamboo leaf extract;
component B: the preservation number is: cctccc NO: salt-tolerant bacillus of M20232276Bacillus halotolerans) Wherein, the consumption of the salt-tolerant bacillus accounts for 0.1 to 1 weight percent of the total weight of the bio-organic fertilizer.
The invention further provides application of the biological organic fertilizer in preventing and treating crop root rot, improving organic carbon content of plant rhizosphere soil, improving nitrogen absorption and utilization rate of plants and improving proline content of plant root systems.
The invention also provides a method for preventing and treating root rot of crops, which comprises the following steps: the deposit numbers are: cctccc NO: the salt-tolerant bacillus of M20232276 and/or the above-mentioned bio-organic fertilizer is applied to the soil.
The invention has the beneficial effects that:
(1) The salt-tolerant bacillus provided by the inventionBacillus halotolerans) Is a natural strain with better field planting capability, and is more beneficial to exerting the biocontrol property of the strain compared with a genetic engineering strain or an induced mutant strain, thereby being capable of obtaining excellent disease control effect.
(2) The biological organic fertilizer provided by the invention can effectively regulate the metabolism of plants, and improve the proline content and the root activity in the root systems of the plants, so that the problems of serious disease occurrence or plant resistance reduction and the like in the growth of the plants can be effectively solved, and the organic carbon content of the rhizosphere soil of the plants can be improved.
(3) The biological organic fertilizer provided by the invention has the characteristics of wide raw material sources, low cost, environment friendliness and the like. Meanwhile, the preparation method of the fertilizer is also very simple and has high storage stability. The selection of the raw material types and the consumption of the fertilizer ensures that the viable count of the salt-tolerant bacillus is higher when the salt-tolerant bacillus is added.
(4) The method for preventing and treating the root rot of the crops is simple and easy to implement, does not have adverse effect on environmental safety, and can promote sustainable development of agricultural production.
Drawings
FIG. 1 shows the salt-tolerant bacillus of the inventionBacillus halotolerans) Is a colony morphology map of (a);
FIG. 2 is a phylogenetic tree of strain YNK-FB0022 of the invention.
Preservation of organisms
The salt-tolerant bacillus provided by the inventionBacillus halotolerans) The classification is named:Bacillus halotoleransYNK-FB0022 was deposited at the China center for type culture Collection, with the address of university of Wuhan, china, and the deposit number of CCTCC NO: m20232276.
Detailed Description
The endpoints and any values of the ranges disclosed herein are not limited to the precise range or value, and are understood to encompass values approaching those ranges or values. For numerical ranges, one or more new numerical ranges may be found between the endpoints of each range, between the endpoint of each range and the individual point value, and between the individual point value, in combination with each other, and are to be considered as specifically disclosed herein.
In the invention, salt-tolerant bacillus YNK-FB0022 and salt-tolerant bacillus CCTCC NO: m20232276 is the same strain, both of which are synonymous, and their names (numbers) are used interchangeably.
The salt-tolerant bacillus YNK-FB0022 of the invention is disclosed in journal microbiology report, and the paper subject name is: screening and identification of three phenolic acid degrading bacteria and its biological activity (https:// doi.org/10.13344/j.
The inventor of the invention separates and obtains a strain of salt-tolerant bacillus during the research processBacillus halotolerans) The strain is named as YNK-FB0022 and is preserved in China Center for Type Culture Collection (CCTCC) at the year 2023, 11 and 20, and the preservation number is CCTCC NO: m20232276. Through further research, when the salt-tolerant bacillus is applied to soil, the root rot of crops can be effectively prevented and treated, the organic carbon content of plant rhizosphere soil is improved, the nitrogen absorption and utilization rate of plants is improved, and the proline content of plant root systems is improved.
Based on this, the first aspect of the present invention provides a deposit number: cctccc NO: salt-tolerant bacillus of M20232276Bacillus halotolerans) The application of the plant growth regulator in preventing and treating crop root rot, improving the organic carbon content of plant rhizosphere soil, improving the nitrogen absorption and utilization rate of plants and improving the proline content of plant root systems.
The inventor of the invention demonstrates in research that the salt-tolerant bacillus provided by the invention can effectively prevent and treat root rot of crops when being applied to soil alone or together with certain organic fertilizer materials, improve the organic carbon content of plant rhizosphere soil, improve the nitrogen absorption and utilization rate of plants and the proline content and root activity of plant roots, thereby promoting plant growth and improving the systemic resistance of plants.
Based on this, a second aspect of the present invention provides a bio-organic fertilizer comprising:
component A: comprises the following components in percentage by weight: 4-6:1-5:0.5-1 of mushroom bran, vetch straw, gibberellic acid and bamboo leaf extract;
component B: comprises 0.1-1% of the total weight of component A and has a deposit number of: cctccc NO: salt-tolerant bacillus of M20232276Bacillus halotolerans）。
When the bio-organic fertilizer prepared by mixing mushroom fungus chaff, vetch straw, gibberellic acid, bamboo leaf extract and the like is applied to soil, the crop root rot can be effectively prevented and treated, the organic carbon content of plant rhizosphere soil is improved, the nitrogen absorption and utilization rate of plants is improved, the proline content and the root activity of plant root systems are improved, and the use amount of chemical pesticides and fertilizers is reduced. The inventor further researches that the salt-tolerant bacillus CCTCC NO provided by the invention is added on the basis of the bio-organic fertilizer prepared by the components: m20232276, the effect thereof can be further enhanced.
In the invention, the raw materials (such as lentinus edodes fungus chaff, sweet potato straw, gibberellic acid, bamboo leaf extract and the like) used as the organic fertilizer are not particularly limited, and can be any related products which can be used for preparing liquid fertilizer in the field, and can be related products obtained commercially or self-prepared according to the prior art.
According to a preferred embodiment of the present invention, wherein the moisture content of the lentinus edodes bran is 10-15 wt%; in the lentinus edodes bran, the content of crude protein is 10-15 wt%, the content of crude fiber is 8-12 wt%, the content of crude fat is 0.1-0.5 wt%, the content of nitrogen-free extract is 55-60 wt%, and the content of calcium is 2-5 wt% based on dry matter; and/or, the water content in the vetch straw is 20-30%, the cellulose content is 55-65% by weight based on dry matter, and the crude protein content is 5-10% by weight; and/or, the bamboo leaf flavone content in the bamboo leaf extract is 10-15% based on dry matter.
Preferably, the salt-tolerant bacillus is used in an amount of 0.1-1 wt% based on the total weight of the bio-organic fertilizer. Preferably 0.3 to 0.8% by weight. More preferably 0.5 to 0.8 wt%.
More preferably, the content of the salt-tolerant bacillus in the bio-organic fertilizer is not less than 1 multiplied by 10 9 cfu/mL. Preferably 1X 10 9 -5×10 12 cfu/mL. More preferably 2X 10 9 -5×10 12 cfu/mL. Further preferably 1X 10 10 -2×10 12 cfu/mL. When the salt-tolerant bacillus is added into the bio-organic fertilizer in the form of a solid bio-organic fertilizer, the content of the salt-tolerant bacillus in the bio-organic fertilizer after the addition is converted according to the proportion of 1mL of the finally obtained bio-organic fertilizer being 1 g.
The third aspect of the invention provides application of the salt-tolerant bacillus and the bio-organic fertilizer in the second aspect in preventing and controlling plant diseases, improving organic carbon content of plant rhizosphere soil, improving nitrogen absorption and utilization rate of plants and improving proline content and root activity of plant roots.
Wherein the plants are eustoma grandiflorum, tomato, okra and angelica sinensis.
Wherein the plant disease is eustoma root rot, tomato root rot, okra root rot and angelica root rot.
In the invention, preventing and controlling diseases means reducing the incidence of diseases, regulating the soil nutrient structure (for example, improving the organic carbon content of soil and the nitrogen absorption and utilization rate of plants), and improving the systemic resistance of plants (for example, improving the proline content of plant root systems, the activity of root systems and the like).
In a fourth aspect, the present invention provides a method for controlling root rot of crops, the method comprising applying the salt tolerant bacillus of the first aspect and/or the bio-organic fertilizer of the second aspect to soil.
That is, the above method includes the following means:
(1) Applying salt-tolerant bacillus or bio-organic fertilizer to soil independently;
(2) The salt-tolerant bacillus and the bio-organic fertilizer are applied together into soil.
The application method of the present invention is not particularly limited, and any method of applying fertilizer in soil, which is commonly used in the art, may be applied to the present invention.
The specific dosage of the salt-tolerant bacillus and the bio-organic fertilizer is not particularly limited, so long as the effects of reducing the disease incidence and improving the plant root system resistance can be achieved.
According to a preferred embodiment of the present invention, wherein, for the above-mentioned modes (1) and (2), the salt-tolerant bacillus and the bio-organic fertilizer are applied in an amount of not less than 1X 10 in terms of the salt-tolerant bacillus 9 cfu/strain/time. Preferably 1X 10 9 -1×10 13 cfu/strain/time. More preferably 3X 10 9 -3×10 11 cfu/strain/time. For example, it may be 3X 10 9 cfu/strain/time, 4X 10 9 cfu/strain/time, 5X 10 9 cfu/strain/time, 6X 10 9 cfu/strain/time, 8×10 9 cfu/strain/time, 1×10 10 cfu/strain/time, 5X 10 10 cfu/strain/time, 8×10 10 cfu/strain/time, 1×10 11 cfu/strain/time, 2X 10 11 cfu/strain/time, 3×10 11 cfu/strain/time, or any intermediate value between any two values mentioned above. The salt-tolerant bacillus is calculated according to the application amount of the organic fertilizer or the bio-organic fertilizer and the amount of the salt-tolerant bacillus contained in the organic fertilizer, and when the salt-tolerant bacillus is added in the form of a solid bio-organic fertilizer in the bio-organic fertilizer, the salt-tolerant bacillus content in the bio-organic fertilizer after the salt-tolerant bacillus is added according to the proportion of 1mL of the finally obtained bio-organic fertilizer which is 1 g.
Preferably, the application frequency of the salt-tolerant bacillus and the bio-organic fertilizer is 15-20 days before planting and is applied for 1 time as a base fertilizer.
In order to make the objects, technical solutions and advantageous effects of the present invention more apparent, preferred embodiments of the present invention will be described in detail below to facilitate understanding by the skilled person.
Example one isolation, screening and preservation of Strain
The embodiment is used for explaining the separation, screening and preservation of the salt-tolerant bacillus YNK-FB0022.
1. Isolation of strains
NA culture medium is adopted in the process of separating and purifying the strain, and the preparation method is as follows: 10.00 g of peptone g, 3.00 g of beef powder g, 5.00g of sodium chloride, 15.00 g of agar with the pH value of 7.30+/-0.12 and steam sterilization at 121 ℃ for 20 min for standby, and separating and purifying a strain of bacteria named YNK-FB0022 from a root soil sample of the Chinese pine collected from the natural protection area of the free mountain country by adopting a dilution coating flat plate method and a flat plate streaking method.
2. Identification of strains
2.1 identification of morphological characteristics and physiological and biochemical characteristics of bacteria
The strain YNK-FS0019 was subjected to physiological and biochemical measurement by referring to "Berger's Manual of bacteria identification" and "Manual of common bacteria System identification", and colony morphology was described.
Colony & cell morphology: FIG. 1 shows the colony morphology of strain YNK-FB0022 on NA medium, and it can be seen that the colony of the strain is nearly circular, the opaque surface is smooth, and milky.
Physiological and biochemical characteristics: YNK-FB0022 colony surface is moist and smooth, opaque, gram staining positive, can utilize maltose, glucose can hydrolyze amylase, methyl red reaction positive, can not hydrolyze fat.
2.2 molecular characterization
Total DNA of strain YNK-FB0022 was extracted by Chelex extraction as a template, 27F (5'-AGAGTTTGATCCTGGCTCAG-3') as an upstream primer, 1492R (5'-TACGGCTACCTTGTTACGACTT-3') as a downstream primer, and 16S rRNA amplification was performed using the reaction system and conditions shown in Table 1.
TABLE 1 PCR System and conditions
The amplified product was subjected to 1% agarose gel electrophoresis (using a gel recovery purification kit manufactured by Guangzhou American biosciences Co., ltd.) and then sent to Beijing Qingqike biosciences Co., ltd.) for sequencing, and the sequencing result was shown as (GenBank accession number OR 523290). Sequencing results were analyzed by comparison in NCBI database (https:// www.ncbi.nlm.nih.gov /) and EZBiocloud database (https:// www.ezbiocloud.net /). Adopting a phylogenetic analysis method, selecting a mode strain 16S rRNA sequence with higher homology as a reference object, carrying out multi-sequence comparison by using Clustal X1.8 software, and calculating the similarity of the sequences of the tested strain and the reference strain. The phylogenetic analysis was performed by excluding the base deletion site, and constructing a phylogenetic tree between the test strain and the reference strain by using MEGA 7.0 by the Neighbor-joining method (Neighbor-joining method). Wherein, the Bootstrap value is set to 1000, and the rest are default values.
FIG. 2 showsThe phylogenetic tree of the drawn strain YNK-FB0022 is shown, from which YNK-FB0022 and salt-tolerant bacillus are seenBacillus halotolerans) LPVF01000003 has the highest homology. Molecular detection results of the strain YNK-FB0022 and detection results of bacterial morphological characteristics and physiological biochemical characteristics are combined, and the strain is identified to be salt-tolerant bacillusBacillus halotolerans)。
3. Preservation of strains
Bacillus halodurans YNK-FB0022 (Bacillus halotolerans) was deposited with the China center for type culture collection, under the classification designation:Bacillus halotoleransYNK-FB0022, the preservation unit address is the university of Wuhan, china, and the preservation number is CCTCC NO: m20232276.
Example two preparation of bioorganic fertilizer
The embodiment is used for explaining the preparation process of the salt-tolerant bacillus YNK-FB0022 bio-organic fertilizer.
The method comprises the following steps:
(1) Carrying out solid culture on the salt-tolerant bacillus in a solid culture medium to obtain test tube species;
(2) Preparing a liquid seed culture medium, and inoculating test tube seeds into the liquid seed culture medium for liquid culture to obtain liquid seeds;
(3) Preparing a liquid fermentation medium, and inoculating liquid seeds into the liquid fermentation medium for fermentation.
In the step (1), the salt-tolerant bacillus is inoculated in a slant inoculation mode, and the solid culture is carried out at the temperature of 25-30 ℃ for 40-50h; the solid medium comprises: 10-12g/L of glucose, 15-20g/L of agar, 3-5g/L of beef extract, 1-3g/L of yeast extract, 10-15g/L of peptone and pH=6.5-7.5.
In the step (2), inoculating test tube seeds into a liquid seed culture medium, and treating the liquid seed culture medium for 45-50 hours under the conditions that the temperature is 25-30 ℃ and the rotating speed is 200-250 r/min; the liquid seed medium comprises: 10-15g/L peptone, 3-5g/L beef extract, 10-12g/L sodium chloride and pH=6.5-7.5.
In this step, the liquid seed medium is sterilized preferably at 120-125℃for 20-30min, cooling, and inoculating 0.5-1.5cm into 100mL liquid seed culture medium 2 Culturing in a shaker at 28-30deg.C for 45-50 hr at 220-250r/min to obtain liquid seed.
In the step (3), inoculating the liquid seeds in a liquid fermentation culture medium according to the inoculum size of 0.05-0.1:1 by volume ratio, and treating for 45-50h under the conditions of 25-30 ℃ and 200-250r/min of rotating speed; the liquid fermentation medium comprises: 20-23g/L of sucrose, 10-15g/L of peptone, 5-8g/L of yeast extract, 3-5g/L of monopotassium phosphate, 5-8g/L of ammonium sulfate, 2-4g/L of calcium carbonate and pH=6.5-7.5.
In this step, the liquid fermentation medium is sterilized preferably at 120-125℃for 20-30min, cooled, inoculated with liquid seeds, and cultured preferably at 28-30℃on a shaker at a rotational speed of 220-250r/min for 45-50h to give a fermentation broth.
The preparation steps further comprise:
diluting the obtained culture solution with fresh NB medium to obtain viable bacteria number of about 2×10 10 cfu/mL of salt-tolerant bacillus liquid microbial inoculum. Drying the liquid microbial inoculum to obtain dry powder preparation with viable count of about 2×10 10 cfu/g。
Example preparation of a three-bio-organic fertilizer
This example is used to illustrate the preparation process of the bio-organic fertilizer provided by the present invention.
The formula of the bio-organic fertilizer is shown in Table 2 in detail, wherein the detection results of the material components of mushroom fungus chaff, vetch straw, gibberellic acid and bamboo leaf extract are shown in Table 1 in detail (wherein the water content is calculated based on the total weight of the material, and the rest components are calculated based on the dry matter in the material). The crude protein content is determined by a Kai-type nitrogen determination method, the crude fiber content is determined by an acid hydrolysis-anthrone colorimetric method, the crude fat content is determined by a Soxhlet extraction method, the nitrogen-free extract content is determined by a near infrared analysis method, and the calcium content is determined by a flame atomic absorption spectrometry method. A bio-organic fertilizer was prepared from example 2. The lentinan is purchased from Shanxi Miruinong biotechnology Co., ltd, the gibberellic acid is purchased from Shanghai Fu's Dai biotechnology Co., ltd, the bamboo leaf extract is purchased from Jiangsu si biotechnology Co., ltd, and the collected stalks of the sweet potato are cut off and mixed and fermented for 3-5 months to obtain the stalk of the sweet potato. The specific preparation method of the bio-organic fertilizer comprises the following steps: raw materials are weighed according to the proportion shown in Table 2, 1L/kg of water is added, and the bio-organic fertilizer is obtained after uniform mixing.
TABLE 2 biological organic fertilizer compositions
TABLE 3 biological organic fertilizer formulation
Example four salt-tolerant bacillus and application Effect of bioorganic fertilizer containing the same on plants
The embodiment is used for explaining the application effect of the salt-tolerant bacillus YNK-FB0022 and the bio-organic fertilizer provided by the invention on plants.
(1) The bio-organic fertilizer used in this example was prepared in example 3, and the bio-organic fertilizer prepared in example 3 was applied to plant rhizosphere soil in the amounts shown in table 3, and then simultaneously cultured under other conditions kept consistent. The specific fertilization mode is as follows: 15-20 days before crop planting, applying the bio-organic fertilizer as a base fertilizer. The dosage is that the salt-tolerant bacillus is not less than 1 multiplied by 10 9 cfu/strain/time; the application frequency of the salt tolerant bacillus or the bio-organic fertilizer is that the salt tolerant bacillus or the bio-organic fertilizer is applied for 1 time as a base fertilizer 15-20 days before planting.
(2) The microbial inoculum prepared in example 2 was applied to plant rhizosphere soil as in example 4 (1), designated as D7.
(3) The control group is not treated at all, and the fertilizer group is conventional fertilizer, wherein urea, calcium superphosphate and potassium sulfate are prepared by themselves, and the weight ratio of N, P, K is 16.7:9.2:21.6.
After fertilization, disease onset conditions are investigated in a harvesting period, control effects are recorded and calculated, plant rhizosphere soil is collected, and plant root systems are collected. Specifically measuring the organic carbon content of the rhizosphere soil of each group of crops, measuring the nitrogen absorption and utilization rate of plants, measuring the proline content and the root activity of the root system of the plants, and measuring the prevention and control effects of diseases.
The root rot disease investigation method comprises the following steps:
level 0: asymptomatic condition
Stage 1: the root of the plant has a small amount of disease spots of 0-10%.
3 stages: part of the lesion spots appear in the root 10-25%.
5 stages: root is completely infected.
7 stages: the plants die.
The morbidity and index were calculated.
Incidence = number of diseased plants/total number of investigated plants 100
Disease index = (Σ (number of disease stages of each stage of disease strain)/investigation of total number of plants of highest stage) 100
The method comprises the steps of determining the organic carbon content of plant rhizosphere soil, and determining the organic carbon (g/kg) content by adopting a potassium dichromate oxidation-spectrophotometry;
the proline content (mug/g) and root activity (mg/g/h) of the plant root system were both determined using a kit purchased from suzhou grissin biotechnology limited, methods of use referred to herein.
Determination method of nitrogen absorption and utilization (NRE) of plants: respectively, measurement test treatment groups (G) N ) And control treatment group (G) O ) The nitrogen absorption amount of the root system of the crop is recorded, and the fertilization amount (N N ) The Kjeldahl nitrogen determination method is adopted for determination, and the nitrogen absorption and utilization rate of the plant is calculated by adopting the following formula: NRE (%) = (G) N -G O ）/N N ×100。
As can be seen from the data in tables 4, 5, 6 and 7, the effects of preventing and controlling crop diseases and improving the organic carbon content of plant rhizosphere soil, the proline content of plant root system, the activity of root system and the nitrogen absorption and utilization rate of plants are stronger than those of a control under the condition of applying the bio-organic fertilizer provided by the invention.
TABLE 4 test conditions and results for prevention and control of root rot of crop with bioorganic fertilizer
TABLE 5 Effect of bioorganic fertilizer application on organic carbon content of crop rhizosphere soil
TABLE 6 Effect of bioorganic fertilizer application on crop root proline content and root vigor
TABLE 7 Effect of Bio-organic fertilizer application on Nitrogen uptake utilization in plants
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (7)
1. The preservation number is: cctccc NO: the application of the salt-tolerant bacillus of M20232276 in preventing and controlling crop root rot, improving the organic carbon content of plant rhizosphere soil, improving the nitrogen absorption and utilization rate of plants and improving the proline content of plant root systems.
2. A biological organic fertilizer is characterized in that: the bio-organic fertilizer comprises the following components:
component A: comprises the following components in percentage by weight: 4-6:1-5:0.5-1 of mushroom bran, vetch straw, gibberellic acid and bamboo leaf extract;
component B: the preservation number is: cctccc NO: the salt-tolerant bacillus of M20232276, wherein the consumption of the salt-tolerant bacillus is 0.1-1 wt% of the total weight of the bio-organic fertilizer.
3. The bio-organic fertilizer according to claim 2, wherein: the moisture content of the mushroom bran is 10-15 wt%; in the lentinus edodes bran, the content of crude protein is 10-15 wt%, the content of crude fiber is 8-12 wt%, the content of crude fat is 0.1-0.5 wt%, the content of nitrogen-free extract is 40-50 wt%, and the content of calcium is 2-5 wt% based on dry matter; and/or, the water content in the vetch straw is 20-30%, the cellulose content is 55-65% by weight based on dry matter, and the crude protein content is 5-10% by weight; and/or, the bamboo leaf flavone content in the bamboo leaf extract is 10-15% based on dry matter.
4. The use of the bio-organic fertilizer according to claim 2 for controlling root rot of crops, increasing organic carbon content of plant rhizosphere soil, increasing nitrogen absorption and utilization rate of plants and increasing proline content of plant root systems.
5. Use according to claim 1 or 4, characterized in that: the root rot is: eustoma grandiflorum root rot, tomato root rot, okra root rot and angelica root rot, and the plant is: eustoma grandiflorum, tomato, okra and angelica sinensis.
6. A method for controlling root rot of crops, which is characterized in that: the deposit numbers are: cctccc NO: m20232276 and/or the bioorganic fertilizer of claim 2 or 3 is applied to the soil.
7. The method according to claim 6, wherein: the application amount of the salt-tolerant bacillus and/or the bio-organic fertilizer is as follows: the effective viable count of salt-tolerant bacillus is not less than 1×10 9 cfu/strain/time; the application frequency of the salt-tolerant bacillus and/or the bio-organic fertilizer is 1 time when the salt-tolerant bacillus and/or the bio-organic fertilizer are used as base fertilizer 15-20 days before planting.
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