CN117840011A - Preparation method of hydrophilic array based on hydrophobic substrate - Google Patents
Preparation method of hydrophilic array based on hydrophobic substrate Download PDFInfo
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- CN117840011A CN117840011A CN202410017890.0A CN202410017890A CN117840011A CN 117840011 A CN117840011 A CN 117840011A CN 202410017890 A CN202410017890 A CN 202410017890A CN 117840011 A CN117840011 A CN 117840011A
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- hydrophilic
- mask
- array
- hydrophobic substrate
- hydrophobic
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- 239000000758 substrate Substances 0.000 title claims abstract description 50
- 230000002209 hydrophobic effect Effects 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title abstract description 11
- 238000000151 deposition Methods 0.000 claims abstract description 26
- 239000000126 substance Substances 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000000463 material Substances 0.000 claims abstract description 11
- 238000000889 atomisation Methods 0.000 claims abstract description 8
- 238000005137 deposition process Methods 0.000 claims abstract description 4
- 238000005229 chemical vapour deposition Methods 0.000 claims abstract description 3
- 239000011148 porous material Substances 0.000 claims abstract description 3
- 239000007921 spray Substances 0.000 claims abstract description 3
- 238000009718 spray deposition Methods 0.000 claims abstract description 3
- 239000000725 suspension Substances 0.000 claims abstract description 3
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 25
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 25
- 239000003153 chemical reaction reagent Substances 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 16
- 229920001223 polyethylene glycol Polymers 0.000 claims description 11
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 10
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 10
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 10
- 108010010803 Gelatin Proteins 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000008273 gelatin Substances 0.000 claims description 7
- 229920000159 gelatin Polymers 0.000 claims description 7
- 235000019322 gelatine Nutrition 0.000 claims description 7
- 235000011852 gelatine desserts Nutrition 0.000 claims description 7
- -1 polydimethylsiloxane Polymers 0.000 claims description 7
- 239000002202 Polyethylene glycol Substances 0.000 claims description 5
- 238000003491 array Methods 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 4
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 4
- 230000008020 evaporation Effects 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 3
- 235000010419 agar Nutrition 0.000 claims description 2
- 238000003486 chemical etching Methods 0.000 claims description 2
- 238000010147 laser engraving Methods 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- 239000007769 metal material Substances 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 238000004080 punching Methods 0.000 claims description 2
- 230000003075 superhydrophobic effect Effects 0.000 claims description 2
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 1
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims 1
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims 1
- 230000008021 deposition Effects 0.000 description 12
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 9
- 229940043267 rhodamine b Drugs 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 6
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000005266 casting Methods 0.000 description 3
- 229920006267 polyester film Polymers 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000004381 surface treatment Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 1
- 239000005662 Paraffin oil Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 235000021167 banquet Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Application Of Or Painting With Fluid Materials (AREA)
Abstract
The invention discloses a preparation method of a hydrophilic array based on a hydrophobic substrate, which comprises the following steps: (1) Covering a mask with a plurality of holes uniformly arranged on a hydrophobic substrate; (2) Depositing a solution or suspension of hydrophilic material into the pores on the mask; wherein the deposition method is ultrasonic atomization or spray or chemical vapor deposition; (3) In the deposition process, a movable mask is arranged on a porous mask, and the shielding area of the movable mask is changed by motor driving and manual movement, so that the control of the amounts of hydrophilic substances at different positions in the array is realized; (4) Removing the mask from the hydrophobic substrate such that hydrophilic species are deposited on the hydrophobic substrate forming an array of hydrophilic regions; the invention realizes the efficient, controllable and stable preparation of the hydrophilic array without complex process steps or equipment.
Description
Technical Field
The invention relates to the technical field of substrate surface treatment, in particular to a preparation method of a hydrophilic array based on a hydrophobic substrate.
Background
A hydrophilic array on a hydrophobic substrate is a structure with specific surface properties that consists of a series of hydrophilic areas distributed on the hydrophobic substrate. This structure can be used for droplet generation, i.e. by injecting a liquid into a hydrophilic region on a hydrophobic substrate, utilizing the liquid surface tension to form stable droplets with Rayleigh-Plateau instability. Droplet generation is an important microfluidic technology that can be used in a variety of fields such as biological analysis, chemical reactions, material synthesis, and the like.
The preparation methods of hydrophilic arrays on hydrophobic substrates are mainly of two types: one is to change the nature of the substrate surface by physical or chemical means so that it has different hydrophilicity and hydrophobicity; another type is the formation of hydrophilic regions by depositing hydrophilic species on a hydrophobic substrate. The former generally requires complex process steps such as etching, photolithography, surface treatment, etc.; the latter is relatively simple but has problems such as selection of deposition materials, control of deposition processes, stability after deposition, and the like. In addition, how to reliably control the substances contained in the liquid drops and the occurrence of reactions in the liquid drops is also an important problem to be solved by the liquid drop array.
Disclosure of Invention
The invention aims to: the invention aims to provide a preparation method of a hydrophilic array based on a hydrophobic substrate, which solves the problems existing in the background technology and realizes efficient, controllable and stable preparation of the hydrophilic array.
The technical scheme is as follows: the invention relates to a preparation method of a hydrophilic array based on a hydrophobic substrate, which comprises the following steps:
(1) Covering a mask with a plurality of holes uniformly arranged on a hydrophobic substrate;
(2) Depositing a solution or suspension of hydrophilic material into the pores on the mask; wherein the deposition method is ultrasonic atomization or spray or chemical vapor deposition;
(3) In the deposition process, a movable mask is arranged on a porous mask, and the shielding area of the movable mask is changed by motor driving and manual movement, so that the control of the amounts of hydrophilic substances at different positions in the array is realized;
(4) Removing the mask from the hydrophobic substrate such that hydrophilic species are deposited on the hydrophobic substrate forming an array of hydrophilic regions;
(5) Repeating the steps (2) to (4), depositing hydrophilic substances with different thicknesses at different positions or in different directions, or depositing different kinds of hydrophilic substances with different thicknesses at the same position, thereby obtaining hydrophilic arrays with different properties or functions.
Further, in the step (1), the hole of the mask is circular, square, triangular or any other shape; the size of the holes is 0.1-5mm, and the spacing is 0.2-10mm.
Further, in the step (1), the hydrophobic substrate is polydimethylsiloxane PDMS, polypropylene PP, polymethyl methacrylate PMMA, or surface-modified glass, a metal material, or a super-hydrophobic material.
Further, in the step (1), the mask is a polymer, metal or glass sheet processed by punching, laser engraving and chemical etching.
Further, in the step (2), the hydrophilic substance is polyethylene glycol, polyvinylpyrrolidone, gelatin, agar or a substance containing a reagent.
The invention relates to a liquid drop generation and reagent release method of a hydrophilic array based on a hydrophobic substrate, which comprises the following steps:
placing the hydrophilic array into aqueous liquid and then taking out;
passing an aqueous liquid through the hydrophilic region;
after droplet generation, the different phase liquids are poured onto the droplet array, preventing evaporation of the droplets in air.
Further, the hydrophilic region contains a reagent, and the reagent can be released into the liquid drop during the liquid drop generation process, so that the transfer or reaction of the reagent is realized.
Further, a slow dissolving substance is deposited on the reagent, so that the reagent is released in the liquid drop at regular time.
The beneficial effects are that: compared with the prior art, the invention has the following remarkable advantages: the method has the advantages that the hydrophilic array is simply, controllably and stably prepared on the hydrophobic substrate, and complex process steps or equipment are not required; the invention can be used for research in multiple fields such as biological analysis, chemical reaction, material synthesis and the like.
Drawings
FIG. 1 is a schematic illustration of the present invention;
FIG. 2 is a schematic diagram of a movable mask of the present invention for achieving quality control of hydrophilic substances at different positions;
fig. 3 is a schematic diagram of the present invention for functionalization of hydrophilic regions via multiple deposition and use of a movable mask.
Description of the embodiments
The technical scheme of the invention is further described below with reference to the accompanying drawings.
Example 1
As shown in fig. 1, an embodiment of the present invention provides a method for preparing a hydrophilic array based on a hydrophobic substrate, including the following steps:
(S1) polydimethylsiloxane PDMS is used as a substrate material, and a PDMS substrate 2 with the thickness of 1mm and the surface being hydrophobic is prepared through a casting method;
(S2) covering a polyester film (mask) 1 with a plurality of holes uniformly arranged on a PDMS substrate; wherein the holes have a diameter of 200 μm and a pitch of 500. Mu.m;
(S3) spraying an aqueous solution containing 0.5% polyethylene glycol PEG and 0.01% fluorescein FITC into the holes on the mask (in the direction of arrow 4 in FIG. 1) by ultrasonic atomization; so that PEG and FITC are deposited on the PDMS substrate, forming hydrophilic areas 3;
(S4) removing the mask from the PDMS substrate, resulting in a hydrophilic array 5 with fluorescent properties;
example 2 as shown in FIG. 2
The embodiment of the invention provides a preparation method of a hydrophilic array based on a hydrophobic substrate, which comprises the following steps:
(S6) taking Polydimethylsiloxane (PDMS) as a substrate material, and preparing a PDMS substrate with the thickness of 1mm and the surface being hydrophobic by a casting method;
(S7) covering a polyester film (mask) with a plurality of holes uniformly arranged on the PDMS substrate; wherein the holes have a diameter of 200 μm and a pitch of 500. Mu.m;
(S8) spraying an aqueous solution containing 0.5% polyethylene glycol PEG and 0.01% fluorescein FITC into the holes on the mask (in the direction of arrow in FIG. 2) by ultrasonic atomization; allowing PEG and FITC to deposit on the PDMS substrate to form hydrophilic areas;
(S9) removing the mask from the PDMS substrate to obtain a hydrophilic array having fluorescent properties;
(S10) repeating the steps (S6) to (S9), and depositing hydrophilic substances with different thicknesses at different positions, specifically as follows:
after the first deposition, the porous mask is not removed, and a layer of motor-driven movable mask 7 is added on the porous mask; the movable mask was controlled to slowly remove the porous mask at a fixed rate in the horizontal direction 7 while simultaneously depositing a solution containing 0.5% polyvinylpyrrolidone (PVP) and 0.01% rhodamine B (RhB) using ultrasonic atomization so that PVP and RhB are deposited on the PDMS substrate forming a PVP and RhB gradient in one direction, resulting in a hydrophilic array 8 with a reagent content gradient.
Example 3 as shown in FIG. 3
The embodiment of the invention provides a preparation method of a hydrophilic array based on a hydrophobic substrate, which comprises the following steps:
(S11) taking Polydimethylsiloxane (PDMS) as a substrate material, and preparing a PDMS substrate with the thickness of 1mm and the surface being hydrophobic by a casting method;
(S12) covering a polyester film (mask) with a plurality of holes uniformly arranged on the PDMS substrate; wherein the holes have a diameter of 200 μm and a pitch of 500. Mu.m;
(S13) spraying an aqueous solution containing 0.5% polyethylene glycol PEG and 0.01% fluorescein FITC into the holes on the mask (in the direction of arrow in FIG. 3) by ultrasonic atomization; allowing PEG and FITC to deposit on the PDMS substrate to form hydrophilic areas;
(S14) removing the mask from the PDMS substrate to obtain a hydrophilic array having fluorescent properties;
(S15) repeating the steps (S11) to (S14), depositing hydrophilic substances having different thicknesses at different positions or in different directions, specifically as follows:
after the first deposition, the porous mask is not removed, and a layer of motor-driven movable mask 7 is added on the porous mask; the movable mask is controlled to slowly move away from the porous mask in the horizontal direction 9 of the fixed rate banquet, and simultaneously, a solution containing 0.5% of polyvinylpyrrolidone (PVP) and 0.01% of rhodamine B (RhB) is deposited by ultrasonic atomization, so that PVP and RhB are deposited on a PDMS substrate, and a gradient of PVP and RhB is formed in one direction for second deposition; after the second deposition, the porous mask is not removed and the movable mask is again covered over the porous mask. The movable mask was slowly removed from the porous mask at a fixed rate in a direction 10 perpendicular to the direction of movement of the movable mask during the second deposition, while simultaneously ultrasonic aerosol deposition of a solution containing 0.5% gelatin and 0.01% enzyme-labeled antibody (ELISA) resulted in the deposition of gelatin and ELISA on the PDMS substrate forming a gradient of gelatin and enzyme-labeled antibody in the other direction, which was the third deposition. In this way, hydrophilic arrays 11 having different properties or functions can be obtained.
Example 4
On the basis of example 3, after each reagent deposition was completed, a layer of gelatin or agar solution which was slowly dissolved was further deposited on the reagent, or gelatin or agar solution was added to each deposited reagent to control the reagent release rate and release delay after droplet generation.
Example 5
Droplet generation and reagent release were achieved using the hydrophilic arrays prepared in examples 1-4. The hydrophilic array is placed in deionized water, cell culture solution, microorganism culture solution, PCR reagent, and then removed, or such liquid is passed through the hydrophilic array to form an array of droplets. (e.g., drop array 6 of FIG. 1). After the droplet generation process, the reagents such as fluorescein, rhodamine B, enzyme-labeled antibodies and the like can be released into the droplets, thereby realizing the transfer or reaction of the reagents. Differences in the fluorescent signal or reaction results in the droplet at different positions or orientations can be observed by fluorescence microscopy. After droplet generation, the droplet array may be further placed in a flat bottom container and paraffin oil or other immiscible phase poured onto the droplet array to prevent evaporation of the droplets in air.
Claims (8)
1. A method for preparing a hydrophilic array based on a hydrophobic substrate, comprising the steps of:
(1) Covering a mask with a plurality of holes uniformly arranged on a hydrophobic substrate;
(2) Depositing a solution or suspension of hydrophilic material into the pores on the mask; wherein the deposition method is ultrasonic atomization or spray or chemical vapor deposition;
(3) In the deposition process, a movable mask is arranged on a porous mask, and the shielding area of the movable mask is changed by motor driving and manual movement, so that the control of the amounts of hydrophilic substances at different positions in the array is realized;
(4) Removing the mask from the hydrophobic substrate such that hydrophilic species are deposited on the hydrophobic substrate forming an array of hydrophilic regions;
(5) Repeating the steps (2) to (4), depositing hydrophilic substances with different thicknesses at different positions or in different directions, or depositing different kinds of hydrophilic substances with different thicknesses at the same position, thereby obtaining hydrophilic arrays with different properties or functions.
2. The method according to claim 1, wherein in the step (1), the holes of the mask are circular, square, triangular or any other shape, the size of the holes is 0.1-5mm, and the pitch is 0.2-10mm.
3. The method according to claim 1, wherein in the step (1), the hydrophobic substrate is polydimethylsiloxane PDMS, polypropylene PP, polymethyl methacrylate PMMA, or surface-modified glass, metal material, or super-hydrophobic material.
4. The method of claim 1, wherein in the step (1), the mask is a polymer, metal or glass sheet processed by punching, laser engraving, chemical etching.
5. The method according to claim 1, wherein in the step (2), the hydrophilic substance is polyethylene glycol, polyvinylpyrrolidone, gelatin, agar or a substance containing a reagent.
6. A droplet generation and reagent release method according to claim 1, comprising the steps of:
placing the hydrophilic array into aqueous liquid and then taking out;
passing an aqueous liquid through the hydrophilic region;
after droplet generation, the different phase liquids are poured onto the droplet array, preventing evaporation of the droplets in air.
7. A hydrophilic array based on a hydrophobic substrate according to claim 6, wherein the hydrophilic region contains reagents which can be released into the droplets during droplet generation to effect transfer or reaction of the reagents.
8. A hydrophilic array based on a hydrophobic substrate according to claim 6, wherein a slow dissolving substance is deposited on the reagents to achieve a timed release of the reagents within the droplets.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410017890.0A CN117840011A (en) | 2024-01-05 | 2024-01-05 | Preparation method of hydrophilic array based on hydrophobic substrate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410017890.0A CN117840011A (en) | 2024-01-05 | 2024-01-05 | Preparation method of hydrophilic array based on hydrophobic substrate |
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| Publication Number | Publication Date |
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| CN117840011A true CN117840011A (en) | 2024-04-09 |
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|---|---|---|---|
| CN202410017890.0A Pending CN117840011A (en) | 2024-01-05 | 2024-01-05 | Preparation method of hydrophilic array based on hydrophobic substrate |
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- 2024-01-05 CN CN202410017890.0A patent/CN117840011A/en active Pending
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