CN117800495A - Method for promoting phosphorus accumulating bacteria to reduce phosphorus element in wastewater by utilizing biodegradable microplastic - Google Patents
Method for promoting phosphorus accumulating bacteria to reduce phosphorus element in wastewater by utilizing biodegradable microplastic Download PDFInfo
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- CN117800495A CN117800495A CN202311830909.5A CN202311830909A CN117800495A CN 117800495 A CN117800495 A CN 117800495A CN 202311830909 A CN202311830909 A CN 202311830909A CN 117800495 A CN117800495 A CN 117800495A
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- phosphorus
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 138
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 138
- 239000011574 phosphorus Substances 0.000 title claims abstract description 138
- 241000894006 Bacteria Species 0.000 title claims abstract description 54
- 229920000426 Microplastic Polymers 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000002351 wastewater Substances 0.000 title claims abstract description 34
- 230000001737 promoting effect Effects 0.000 title claims abstract description 27
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 claims abstract description 39
- 229920000903 polyhydroxyalkanoate Polymers 0.000 claims abstract description 39
- 239000002245 particle Substances 0.000 claims abstract description 28
- 229920000747 poly(lactic acid) Polymers 0.000 claims abstract description 27
- 239000004626 polylactic acid Substances 0.000 claims abstract description 27
- 208000034530 PLAA-associated neurodevelopmental disease Diseases 0.000 claims abstract description 3
- 229920013639 polyalphaolefin Polymers 0.000 claims abstract description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 18
- 239000010935 stainless steel Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 241000223651 Aureobasidium Species 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 11
- 241000589291 Acinetobacter Species 0.000 claims description 9
- 239000008187 granular material Substances 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 9
- 238000012258 culturing Methods 0.000 abstract description 8
- 238000002156 mixing Methods 0.000 abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 6
- 229910052799 carbon Inorganic materials 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 2
- 238000004065 wastewater treatment Methods 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 13
- 239000002609 medium Substances 0.000 description 12
- 239000010865 sewage Substances 0.000 description 9
- 241000235058 Komagataella pastoris Species 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 241000223678 Aureobasidium pullulans Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000012856 weighed raw material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for promoting phosphorus accumulating bacteria to reduce phosphorus element in wastewater by utilizing biodegradable microplastic, belonging to the technical field of wastewater treatment. Comprises mixing and culturing phosphorus accumulating bacteria (PAOs) and pretreated biodegradable microplastic, placing into a container, and adding into wastewater to reduce phosphorus element in the wastewater; wherein the biodegradable microplastic comprises one or two of polylactic acid PLA and polyhydroxyalkanoate PHA. The invention can provide additional carbon source for the phosphorus accumulating bacteria by utilizing the biodegradable microplastic, enhance the activity of the phosphorus accumulating bacteria, enable the biodegradable microplastic to be attached by microorganisms more easily, provide more attachment points for the phosphorus accumulating bacteria by adding the biodegradable microplastic, and effectively improve the phosphorus absorbing capacity of the phosphorus accumulating bacteria by adjusting the type and the particle size of the biodegradable microplastic.
Description
Technical Field
The invention belongs to the technical field of wastewater treatment, and particularly relates to a method for promoting phosphorus accumulating bacteria to reduce phosphorus elements in wastewater by utilizing biodegradable microplastic.
Background
Phosphorus is the most important factor in water eutrophication, so that removal of phosphorus from water is particularly important in preventing water eutrophication. At present, the existing sewage treatment plant mostly adopts a biological dephosphorization technology, is mainly completed by leading phosphorus accumulating bacteria, and removes phosphorus in wastewater by utilizing the characteristic that phosphorus is excessively absorbed under an aerobic condition. However, in practical situations, the phosphorus absorption capacity of phosphorus accumulating bacteria is affected by various factors, so that effluent water is difficult to meet the discharge standard of phosphorus.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description summary and in the title of the application, to avoid obscuring the purpose of this section, the description summary and the title of the invention, which should not be used to limit the scope of the invention.
The present invention has been made in view of the above and/or problems occurring in the prior art.
Therefore, the invention aims to overcome the defects in the prior art and provide a method for promoting phosphorus accumulating bacteria to reduce phosphorus element in wastewater by utilizing biodegradable microplastic.
In order to solve the technical problems, the invention provides the following technical scheme: comprising the steps of (a) a step of,
after mixed culture of phosphorus accumulating bacteria (PAOs) and pretreated biodegradable microplastic, placing the mixture into a container and putting the container into wastewater to reduce phosphorus element in the wastewater;
wherein the biodegradable microplastic comprises one or two of polylactic acid PLA and polyhydroxyalkanoate PHA.
As a preferable scheme of the method for promoting the phosphorus accumulating bacteria to reduce the phosphorus element in the wastewater by utilizing the biodegradable microplastic, the invention comprises the following steps: the phosphorus accumulating bacteria is one of the group consisting of the aureobasidium album and the acinetobacter rouxii.
As a preferable scheme of the method for promoting the phosphorus accumulating bacteria to reduce the phosphorus element in the wastewater by utilizing the biodegradable microplastic, the invention comprises the following steps: the pretreatment is cleaning with sterilized water.
As a preferable scheme of the method for promoting the phosphorus accumulating bacteria to reduce the phosphorus element in the wastewater by utilizing the biodegradable microplastic, the invention comprises the following steps: the particle size of the biodegradable microplastic is 1-3 mm, and the particles are in round granules.
As a preferable scheme of the method for promoting the phosphorus accumulating bacteria to reduce the phosphorus element in the wastewater by utilizing the biodegradable microplastic, the invention comprises the following steps: the mixed culture is culture in an enrichment medium.
As a preferable scheme of the method for promoting the phosphorus accumulating bacteria to reduce the phosphorus element in the wastewater by utilizing the biodegradable microplastic, the invention comprises the following steps: the enrichment medium comprises glucose, peptone, yeast powder, na-glutamate, KH 2 PO 4 ，(NH 4 ) 2 SO 4 ，MgSO 4 ·7H 2 O。
As a preferable scheme of the method for promoting the phosphorus accumulating bacteria to reduce the phosphorus element in the wastewater by utilizing the biodegradable microplastic, the invention comprises the following steps: the culture is shake culture on a shaking table at 25-40 ℃ and 120-180 r/min.
As a preferable scheme of the method for promoting the phosphorus accumulating bacteria to reduce the phosphorus element in the wastewater by utilizing the biodegradable microplastic, the invention comprises the following steps: the time of the culture is 3-5 d.
As a preferable scheme of the method for promoting the phosphorus accumulating bacteria to reduce the phosphorus element in the wastewater by utilizing the biodegradable microplastic, the invention comprises the following steps: every 1×10 6 0.5-1.5 g of biodegradable microplastic is added into the phosphorus accumulating bacteria.
As a preferable scheme of the method for promoting the phosphorus accumulating bacteria to reduce the phosphorus element in the wastewater by utilizing the biodegradable microplastic, the invention comprises the following steps: the container is a stainless steel ball, the surface of the container is provided with holes, and the aperture is 0.5-1.2 mm.
The invention has the beneficial effects that:
the phosphorus absorption capacity of the phosphorus accumulating bacteria is easily influenced by the content of organic matters in the wastewater, the biodegradable microplastic can be used for providing an additional carbon source for the phosphorus accumulating bacteria, the activity of the phosphorus accumulating bacteria is enhanced, the biodegradable microplastic is easier to adhere to microorganisms, the biodegradable microplastic is added for providing more adhering points for the phosphorus accumulating bacteria, the phosphorus absorption capacity of the phosphorus accumulating bacteria can be effectively improved by adjusting the type and the particle size of the biodegradable microplastic, and compared with the phosphorus accumulating microphylla, the phosphorus removal rate of the polyhydroxyfatty acid PHA with the particle size of 1mm is improved by 74.1%, and the treated wastewater is effectively ensured to meet the phosphorus emission standard.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:
FIG. 1 shows the phosphorus removal rate of a 1mm particle size microplastic combined with phosphorus accumulating bacteria.
FIG. 2 shows the phosphorus removal rate of the combination of a microplastic with a particle size of 3mm and phosphorus accumulating bacteria.
FIG. 3 is a schematic representation of a medium in which microplastic having a particle size of 1mm is combined with phosphorus accumulating bacteria.
FIG. 4 is a schematic representation of a medium in which microplastic having a particle size of 3mm is combined with phosphorus accumulating bacteria.
Detailed Description
In order that the above-recited objects, features and advantages of the present invention will become more apparent, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present invention is not limited to the specific embodiments disclosed below.
Further, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic can be included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Preparing an enrichment medium:
1) Weighing the following raw materials according to the following formula: glucose 0.5g, peptone 0.5g, yeast powder 0.5g, na-glutamate 0.5g, KH 2 PO 4 0.5g，(NH 4 ) 2 SO 4 0.1g，MgSO 4 ·7H 2 O 0.1g；
2) Putting the weighed raw materials into ultrapure water, stirring uniformly until the raw materials are completely dissolved, and adding the ultrapure water to 1000mL to ensure that the PH is 7.0;
3) Autoclaving at 121deg.C for 20min to obtain enriched culture medium.
The phosphorus removal rate is calculated by the following steps:
the total phosphorus content in the water was measured by ammonium molybdate spectrophotometry (GB 11893-89) and the phosphorus removal rate was calculated.
Dephosphorization rate= (a) TP -B TP )/A Tp X 100%; wherein A is TP For total phosphorus content of the water feed, B TP Is the total phosphorus content of the effluent.
The phosphorus accumulating moon fungus used by the invention is purchased from Beijing North Nakau biological technology institute with the number BNCC337414.
The Acinetobacter rouxii used in the invention is purchased from Beijing North Nakau biological technology institute with the number BNCC139088.
The diameter of the stainless steel ball used in the invention is 9cm, and the aperture of the surface is 0.8mm.
In the bioreactor of the Changzhou Wu Na sewage treatment plant, which is selected by the invention, the total phosphorus content is 10-15 mg/L, and the reactor operates normally after the stainless steel ball is placed.
The raw materials used in the invention are commonly and commercially available in the art unless otherwise specified.
Example 1
The embodiment provides a method for promoting phosphorus accumulating bacteria to reduce phosphorus element in wastewater by utilizing biodegradable microplastic.
1) Selecting polyhydroxyalkanoate PHA with the particle size of 1mm, and cleaning the PHA with sterilized water for 3 times, wherein the particles are in round granules;
2) Take 10 6 Mixing the Aureobasidium album with 1 g polyhydroxyalkanoate PHA with particle diameter of 1mm, adding into enrichment medium, culturing on shaking table at 30deg.C and 150r/min for 5 days, and filling with stainless steel ball;
3) Stainless steel balls are put into a bioreactor of a Changzhou Wu Na sewage treatment plant;
4) The total phosphorus content in the bioreactor was measured and the phosphorus removal rate was calculated.
Example 2
This embodiment differs from embodiment 1 in that: the polyhydroxyalkanoate PHA is replaced by polylactic acid PLA, specifically:
1) Polylactic acid PLA is selected, the grain diameter is 1mm, the grains are in round granules, and the grains are washed with sterilized water for 3 times;
2) Take 10 6 Mixing the phosphorus accumulating aureobasidium parvum with 1 gram of polylactic acid PLA with the particle size of 1mm, adding the mixture into an enrichment medium, culturing the mixture on a shaking table at 30 ℃ for 5 days at 150r/min, and filling the mixture with stainless steel balls;
3) Stainless steel balls are put into a bioreactor of a Changzhou Wu Na sewage treatment plant;
4) The total phosphorus content in the bioreactor was measured and the phosphorus removal rate was calculated.
Example 3
This embodiment differs from embodiment 1 in that: the polyhydroxyalkanoate PHA is replaced by polylactic acid PLA with the grain diameter of 3mm, and the method specifically comprises the following steps:
1) Polylactic acid PLA is selected, the grain diameter is 1mm, the grains are in round granules, and the grains are washed with sterilized water for 3 times;
2) Take 10 6 Mixing the phosphorus accumulating aureobasidium parvum with 1 gram of polylactic acid PLA with the particle size of 3mm, adding the mixture into an enrichment medium, culturing the mixture on a shaking table at 30 ℃ for 5 days at 150r/min, and filling the mixture with stainless steel balls;
3) Stainless steel balls are put into a bioreactor of a Changzhou Wu Na sewage treatment plant;
4) The total phosphorus content in the bioreactor was measured and the phosphorus removal rate was calculated.
Example 4
This embodiment differs from embodiment 1 in that: the particle size of polyhydroxyalkanoate PHA was adjusted to 3mm, specifically:
1) Selecting polyhydroxyalkanoate PHA with the particle size of 3mm, and cleaning the PHA with sterilized water for 3 times, wherein the particles are in round granules;
2) Take 10 6 Mixing the Aureobasidium album with 1 g polyhydroxyalkanoate PHA with particle diameter of 3mm, adding into enrichment medium, culturing at 30deg.C on shaking table of 150r/min for 5 days, and filling with stainless steel ball;
3) Stainless steel balls are put into a bioreactor of a Changzhou Wu Na sewage treatment plant;
4) The total phosphorus content in the bioreactor was measured and the phosphorus removal rate was calculated.
Example 5
The embodiment is used for exploring the influence of the variety of the change bacteria on the phosphorus removal rate, and specifically comprises the following steps:
the Aureobasidium phosphate used in examples 1 to 4 was replaced with Acinetobacter rouxii.
At least 5 sets of parallel tests were performed in examples 1 to 5, and the phosphorus removal rates in examples 1 to 5 were recorded, and the results are shown in table 1.
TABLE 1
From the results in table 1, it can be seen that: the phosphorus removal rate achieved by the embodiment of the invention is very high, because the biodegradable microplastic can provide additional carbon sources for the phosphorus accumulating bacteria, and enhance the activity of the phosphorus accumulating bacteria, thereby promoting the phosphorus removal effect, and especially when polyhydroxyalkanoate PHA with the particle size of 1mm is added, the phosphorus removal rate can reach 62.3 percent at most. Meanwhile, the biodegradable micro plastic has larger specific surface area and pore structure, is favorable for the attachment and growth of phosphorus accumulating bacteria, and further improves the phosphorus removal efficiency. The phosphorus removal rate of the phosphorus accumulating aurea is superior to that of the Acinetobacter rouxii, because the phosphorus accumulating aurea has higher activity and efficiency in the phosphorus removal process, and can utilize compounds such as polymerized phosphate and the like as an endogenous carbon source to convert the compounds into inorganic phosphate through biochemical reaction, thereby realizing the phosphorus removal effect.
Comparative example 1
Comparative example 1 based on example 1, comparative example 1 differs from example 1 in that polyhydroxyalkanoate PHA is not added, specifically:
1) Take 10 6 Adding the Aureobasidium phosphorus bacteria into an enrichment medium, culturing on a shaking table at 30 ℃ and 150r/min for 5 days, and filling the culture medium with stainless steel balls;
2) Stainless steel balls are put into a bioreactor of a Changzhou Wu Na sewage treatment plant;
3) The total phosphorus content in the bioreactor was measured and the phosphorus removal rate was calculated.
Comparative example 2
Comparative example 2 is based on example 1, and comparative example 2 differs from example 1 in that: polylactic acid PLA with a grain diameter of 5mm is used, and concretely comprises the following steps:
1) Polylactic acid PLA with the grain diameter of 5mm is selected, the grains are in round granules, and the grains are washed with sterilized water for 3 times;
2) Take 10 6 Mixing the phosphorus accumulating aureobasidium parvum with 1 gram of polylactic acid PLA with the particle size of 5mm, adding the mixture into an enrichment medium, culturing the mixture on a shaking table at 30 ℃ for 5 days at 150r/min, and filling the mixture with stainless steel balls;
3) Stainless steel balls are put into a bioreactor of a Changzhou Wu Na sewage treatment plant;
4) The total phosphorus content in the bioreactor was measured and the phosphorus removal rate was calculated.
Comparative example 3
Comparative example 3 is based on example 1, and comparative example 3 differs from example 1 in that: the particle size of polyhydroxyalkanoate PHA is adjusted to be 5mm, and the method specifically comprises the following steps:
1) Selecting polyhydroxyalkanoate PHA with the grain diameter of 5mm, and cleaning the PHA with sterilized water for 3 times, wherein the particles are in round granules;
2) Take 10 6 Mixing the Aureobasidium album with 1 g polyhydroxyalkanoate PHA with the grain diameter of 5mm, adding into enrichment medium, culturing on a shaking table at 30deg.C and 150r/min for 5 days, and filling with stainless steel balls; the method comprises the steps of carrying out a first treatment on the surface of the
3) Stainless steel balls are put into a bioreactor of a Changzhou Wu Na sewage treatment plant;
4) The total phosphorus content in the bioreactor was measured and the phosphorus removal rate was calculated.
Comparative example 4
Comparative example 4 was conducted to investigate the effect of the type of the altering bacteria on the phosphorus removal rate, specifically:
the Aureobasidium phosphate used in comparative examples 2 to 3 was replaced with Acinetobacter rouxii.
The phosphorus removal rates of comparative examples 1 to 4 were recorded and compared with example 1, and the results are shown in table 2.
TABLE 2
As can be seen from Table 2, compared with adding only the P.pastoris, the phosphorus removal rate of the polyhydroxyalkanoate PHA with the particle size of 1mm is improved by 74.1%, because the PHA is used as a biodegradable plastic, has the characteristic of large specific surface area, can provide more attachment points for the P.pastoris, and the microplastic with the small particle size is more easily utilized and decomposed by the P.pastoris to provide an additional carbon source, thereby enhancing the activity and promoting the phosphorus removal effect. Meanwhile, PHA has larger specific surface area and pore structure, is favorable for the adhesion and growth of the phosphorus accumulating crescent bacteria, and further improves the phosphorus removal efficiency. When using Acinetobacter rouxii and adding polylactic acid PLA with a particle size of 5mm, the phosphorus removal rate is instead reduced by 25% compared to adding only the P.rouxii, because Acinetobacter rouxii is not suitable for phosphorus removal by using polylactic acid PLA as a carbon source, and polylactic acid PLA with a particle size of 5mm has a certain limit on the adhesion and utilization of Acinetobacter rouxii, resulting in a reduction in the phosphorus removal rate. Therefore, the PHA can improve the dephosphorization efficiency of the phosphorus accumulation aureobasidium pullulans, reduce the pollutant emission and have important significance for environmental protection.
It should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted without departing from the spirit and scope of the technical solution of the present invention, which is intended to be covered in the scope of the claims of the present invention.
Claims (10)
1. A method for promoting phosphorus accumulating bacteria to reduce phosphorus element in wastewater by utilizing biodegradable microplastic is characterized in that: comprising the steps of (a) a step of,
after mixed culture of phosphorus accumulating bacteria (PAOs) and pretreated biodegradable microplastic, placing the mixture into a container and putting the container into wastewater to reduce phosphorus element in the wastewater;
wherein the biodegradable microplastic comprises one or two of polylactic acid PLA and polyhydroxyalkanoate PHA.
2. The method for promoting phosphorus bacteria to reduce phosphorus in wastewater by using biodegradable microplastic according to claim 1, wherein the method comprises the following steps: the phosphorus accumulating bacteria is one of the group consisting of the aureobasidium album and the acinetobacter rouxii.
3. The method for promoting phosphorus bacteria to reduce phosphorus in wastewater by using biodegradable microplastic according to claim 1, wherein the method comprises the following steps: the pretreatment is cleaning with sterilized water.
4. The method for promoting phosphorus bacteria to reduce phosphorus in wastewater by using biodegradable microplastic according to claim 1, wherein the method comprises the following steps: the particle size of the biodegradable microplastic is 1-3 mm, and the particles are in round granules.
5. The method for promoting phosphorus bacteria to reduce phosphorus in wastewater by using biodegradable microplastic according to claim 1, wherein the method comprises the following steps: the mixed culture is culture in an enrichment medium.
6. The method for promoting phosphorus bacteria to reduce phosphorus in wastewater by using biodegradable microplastic according to claim 1, wherein the method comprises the following steps: the enrichment medium comprises glucose, peptone, yeast powder, na-glutamate, KH 2 PO 4 ，(NH 4 ) 2 SO 4 ，MgSO 4 ·7H 2 O。
7. The method for promoting phosphorus bacteria to reduce phosphorus in wastewater by using biodegradable microplastic according to claim 1, wherein the method comprises the following steps: the culture is shake culture on a shaking table at 25-40 ℃ and 120-180 r/min.
8. The method for promoting phosphorus bacteria to reduce phosphorus in wastewater by using biodegradable microplastic according to claim 1, wherein the method comprises the following steps: the time of the culture is 3-5 d.
9. The method for promoting phosphorus bacteria to reduce phosphorus in wastewater by using biodegradable microplastic according to claim 1, wherein the method comprises the following steps: every 1×10 6 0.5-1.5 g of biodegradable microplastic is added into the phosphorus accumulating bacteria.
10. The method for promoting phosphorus bacteria to reduce phosphorus in wastewater by using biodegradable microplastic according to claim 1, wherein the method comprises the following steps: the container is a stainless steel ball, the surface of the container is provided with holes, and the aperture is 0.5-1.2 mm.
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