CN117740693A

CN117740693A - Micro-spectrophotometer

Info

Publication number: CN117740693A
Application number: CN202311563661.0A
Authority: CN
Inventors: 左渡海
Original assignee: Individual
Current assignee: Individual
Priority date: 2023-11-22
Filing date: 2023-11-22
Publication date: 2024-03-22

Abstract

The invention relates to the technical field of spectrophotometers, and provides a micro-spectrophotometer, which at least comprises: the sample measuring piece is provided with a cleaning liquid accommodating groove and a capillary channel which are communicated with each other; wherein the part of the capillary channel for adapting to the slit is a sample measurement channel; after the liquid sample is added from the inlet of the capillary channel, the liquid sample can flow towards the outlet of the capillary channel under the action of capillary action and fills the capillary channel, and the liquid sample can keep the sample at the outlet of the capillary channel in a hanging state without dripping under the action of surface tension so as to fill the sample to be measured in the sample measuring channel; and the negative pressure device can force the cleaning liquid in the cleaning liquid containing groove to flow through the capillary channel so as to flush the capillary channel. The micro spectrophotometer can fully fill the sample to be measured in the sample measuring channel by utilizing the capillary action and the surface tension action of liquid, and can quickly evaporate the residual cleaning liquid in the capillary channel before the next sample adding.

Description

Micro-spectrophotometer

Technical Field

The invention relates to the technical field of spectrophotometers, in particular to a micro-spectrophotometer.

Background

Spectrophotometry is one of the commonly used biochemical test methods and is widely applied to rapid quantitative detection of samples such as sugar, nucleic acid, enzyme or protein.

The micro-spectrophotometer in the prior art is provided with a containing groove, a first measuring unit and a second measuring unit, measuring end faces on the first measuring unit and the second measuring unit extend into the containing groove from the lateral direction, a preset gap is formed between the two measuring end faces, and a sample to be detected is adsorbed in the preset gap to form a liquid column for detection during detection. After detection, adding a cleaning solution into the accommodating groove, soaking the measuring end face through the cleaning solution to digest the sample to be detected, and then discharging the cleaning solution through a liquid outlet on the accommodating groove. However, a part of the cleaning liquid remains on the wetted measuring end surface, and a new sample to be measured is diluted in the next detection, which affects the measurement accuracy.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to overcome the defect that after the micro-spectrophotometer in the prior art is cleaned, a part of cleaning liquid remains on the wetted measuring end surface, and a new sample to be measured is diluted to influence the measuring accuracy in the next detection, so that the micro-spectrophotometer is provided.

In order to solve the technical problems, the technical scheme of the invention is as follows:

the invention provides a micro-spectrophotometer, comprising at least:

the device comprises a sample measuring piece, a sample storage device and a sample storage device, wherein a cleaning liquid containing groove and a capillary channel which are communicated are arranged on the sample measuring piece, and the cleaning liquid containing groove is positioned at the upstream of the capillary channel; wherein the part of the capillary channel for adapting to the slit is a sample measuring channel; after the liquid sample is added from the inlet of the capillary channel, the liquid sample can flow towards the outlet of the capillary channel and fully fill the capillary channel under the action of capillary action, and the liquid sample can keep the sample at the outlet of the capillary channel in a hanging and non-dripping state under the action of surface tension so as to form a stable sample to be measured in the sample measuring channel; the inlet of the negative pressure device is communicated with the outlet of the capillary channel, the negative pressure device can force the cleaning liquid in the cleaning liquid containing groove to flow through the capillary channel so as to flush the capillary channel, and the cleaning liquid remained in the capillary channel is quickly evaporated by utilizing the fast flowing air after flushing.

Further, the sample measuring piece comprises a first columnar part and a second columnar part which are connected; the cleaning liquid containing groove is arranged on the end face of the first columnar part far away from the second columnar part; the capillary passage is provided inside the second cylindrical portion.

Further, the cleaning solution accommodating groove is a groove with a conical structure, the top opening of the cleaning solution accommodating groove is a cleaning solution inlet, and external cleaning solution can enter the cleaning solution accommodating groove through the cleaning solution inlet.

Further, the micro-spectrophotometer also comprises a slit which is in a plate-shaped structure, and a slit is arranged on the plate surface of the slit; the periphery of the second cylindrical part is provided with two light-transmitting planes which are arranged opposite to each other, each light-transmitting plane is provided with a slit, and the slits on the two opposite slits are aligned, so that light can enter the sample measuring channel through one slit and then be emitted through the other slit.

Further, the negative pressure device comprises a negative pressure pump and a negative pressure channel; one end of the negative pressure channel is communicated with the outlet of the capillary channel, and the other end of the negative pressure channel is communicated with the negative pressure pump.

Further, the negative pressure device also comprises a negative pressure tank and a control valve; the air inlet of the negative pressure tank is connected with the negative pressure channel, and the air outlet of the negative pressure tank is connected with the negative pressure pump; the air inlet of the negative pressure tank is provided with the control valve.

Further, the negative pressure device also comprises a negative pressure joint; one end of the negative pressure connector is connected with the negative pressure channel, and the other end of the negative pressure connector is connected with the air inlet of the negative pressure tank.

Further, the sample measuring member further includes a third columnar portion connected to an end of the second columnar portion remote from the first columnar portion, the capillary passage extending to an end face of the third columnar portion toward a direction remote from the cleaning liquid accommodating groove; the third columnar part at least partially stretches into the negative pressure channel, and a sealing ring is arranged between the outer side wall of the third columnar part and the inner side wall of the negative pressure channel.

Further, the micro-spectrophotometer further comprises a sample adding device, wherein a liquid dropping port of the sample adding device can extend to the capillary channel through the cleaning liquid containing groove so as to drop a sample to be detected into the junction of the capillary channel and the cleaning liquid containing groove.

The technical scheme of the invention has the following advantages:

the micro-spectrophotometer provided by the invention can form a stable liquid column for detection in the capillary channel, and because the cleaning liquid containing groove is positioned at the upstream of the capillary channel, when the capillary channel is required to be cleaned after detection is finished, only the cleaning liquid is required to be added into the cleaning liquid containing groove, under the action of the negative pressure device, the cleaning liquid can be continuously flushed through the capillary channel, after the flushing is finished (the cleaning liquid in the cleaning liquid containing groove is completely consumed), external air can be quickly sucked into the capillary channel by utilizing the negative pressure effect, the residual cleaning liquid in the capillary channel can be quickly carried away by the fast flowing air current, the cleaning liquid is quickly evaporated before the next sample is added, the dilution or pollution of the newly added sample to be detected, which is remained before, is prevented, and the detection precision is improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a cross-sectional view of a sample measurement member in a micro-spectrophotometer in accordance with an embodiment of the present invention;

FIG. 2 is an overall schematic view of a sample measurement member in a micro-spectrophotometer according to an embodiment of the present invention;

FIG. 3 is a schematic diagram showing the positional relationship between a slit and a sample measurement member in a micro-spectrophotometer according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of a micro-spectrophotometer in an embodiment of the present invention;

FIG. 5 is a cross-sectional view of a micro-spectrophotometer in an embodiment of the present invention;

FIG. 6 is a schematic diagram showing the positional relationship of each part in the micro-spectrophotometer according to the embodiment of the present invention.

1. A sample measurement member; 2. a cleaning liquid accommodating groove; 3. a capillary channel; 4. a first columnar portion; 5. a second column section; 6. a third columnar portion; 7. a cleaning liquid inlet; 8. a light-transmitting plane; 9. a slit; 10. a slit; 11. a seal ring; 12. a sample addition device; 13. a light source; 14. an optical fiber; 15. a negative pressure joint; 16. a mounting groove; 17. a negative pressure channel; 18. a control valve; 19. a negative pressure tank; 20. a negative pressure pump.

Detailed Description

The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

In addition, the technical features of the different embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.

As shown in fig. 1, this embodiment provides a micro-spectrophotometer, which at least includes: the sample measuring member 1 may be made of transparent glass and has light transmittance. The sample measuring piece 1 is provided with a cleaning liquid containing groove 2 and a capillary channel 3 which are communicated, and the cleaning liquid containing groove 2 is positioned at the upstream of the capillary channel 3; wherein the part of the capillary channel for adapting to the slit is a sample measurement channel; the size of the capillary channel 3 can be designed according to actual needs, so that liquid sample can flow towards the outlet of the capillary channel 3 and fully fill the capillary channel 3 under capillary action after being added from the inlet of the capillary channel 3, and the liquid sample can keep the sample at the outlet of the capillary channel 3 in a hanging state without dripping under the action of surface tension so as to fully fill the sample to be detected in the sample measuring channel, thereby ensuring the detection effect; the inlet of the negative pressure device is communicated with the capillary channel 3, the negative pressure device can force the cleaning liquid in the cleaning liquid containing groove 2 to flow through the capillary channel 3 to flush the capillary channel 3, and the capillary channel 3 is quickly evaporated by using fast flowing air flow after flushing. Wherein, be provided with the mounting groove 16 that suits with sample measurement spare 1 on the laboratory bench of micro-spectrophotometer, during the use, it can to install this sample measurement spare 1 in the mounting groove 16 on the laboratory bench.

It should be noted that the cause of the sample to be measured being able to flow down in the capillary channel 3 is capillary action. Capillary action is due to the interaction forces that exist between the liquid molecules, which force causes the liquid to flow up or down the tubules. In this case, the capillary channel 3 is thin, so that the interaction force between the water molecules causes the sample to be measured to flow down the hole. The reason why the flow of the sample to be measured to the lower end of the capillary channel 3 is stopped is the effect of the surface tension of the liquid. The surface tension of a liquid refers to the interaction force between molecules on the surface of the liquid, and the interaction force can enable the surface of the liquid to form a tension film. In this case, the water surface tension will prevent the sample to be measured from continuing to flow when water flows to the lower end of the capillary channel 3. Moreover, when the sample to be measured flows to the lower end of the capillary channel 3, the end face forms a circular water droplet. The size of the water droplets depends on the diameter of the sample measurement 1 and the diameter of the capillary channel 3. The larger the size of the water droplets, the greater the resistance to water flowing to the lower end of the capillary channel 3, so the slower the speed of water flow. If it is desired to continue the flow of water after it has reached the lower end of the capillary channel 3, the pressure of the water may be increased. For example, more water may be added to the capillary channel 3, or the sample measurement member 1 may be tilted downward.

According to the micro-spectrophotometer provided by the embodiment, a liquid column for detection can be formed in the capillary channel 3, and because the cleaning liquid accommodating groove 2 is located at the upstream of the capillary channel 3, when the capillary channel 3 needs to be cleaned after detection is completed, cleaning liquid is only required to be added into the cleaning liquid accommodating groove 2, under the action of the negative pressure device, the cleaning liquid can be continuously flushed through the capillary channel 3, after flushing is completed (the cleaning liquid in the cleaning liquid accommodating groove 2 is completely consumed), external air can be quickly sucked into the capillary channel by utilizing the negative pressure effect, the residual cleaning liquid in the capillary channel can be quickly taken away by the fast flowing air flow, the cleaning liquid is quickly evaporated before the next sample adding, the newly filled sample to be detected is prevented from being diluted or polluted by the residual cleaning liquid before, and the detection precision is favorably improved.

As shown in fig. 2, specifically, the sample measurement member 1 includes a first columnar portion 4 and a second columnar portion 5 connected; the cleaning liquid accommodating groove 2 is provided on an end surface of the first columnar portion 4 away from the second columnar portion 5; the capillary channel 3 is provided inside the second cylindrical portion 5. For example, the first columnar portion 4 and the second columnar portion 5 may each have a cylindrical structure, and the diameter of the first columnar portion 4 may be smaller than the diameter of the second columnar portion 5. Preferably, the cleaning solution containing groove 2 is a groove with a conical structure, the capillary channel 3 is a pore canal with a circular section, and the two grooves can be coaxially arranged. Wherein the top opening of the cleaning liquid accommodating groove is a cleaning liquid inlet, and external cleaning liquid can enter the cleaning liquid accommodating groove 2 through the cleaning liquid inlet 7. For example, a hole may be formed in the side wall of the laboratory bench, and in the vertical direction, the position of the hole is higher than that of the sample measuring member 1, and a pipe may extend into the hole and extend to above the top opening of the cleaning liquid accommodating tank 2, and the cleaning liquid in the pipe may enter the cleaning liquid accommodating tank 2 from the top opening of the cleaning liquid accommodating tank 2 when flowing out. As shown in fig. 3, 4 and 5, the micro-spectrophotometer further includes a slit 9, the slit is in a plate-shaped structure, a slit 10 is arranged on the plate surface of the slit 9, and the rest areas except the slit 10 on the slit 9 are light-proof; the periphery of the second cylindrical portion 5 is provided with two light-transmitting planes 8 which are arranged opposite to each other, each light-transmitting plane 8 can be provided with a slit 9 through a screw or an adhesive, the slits 10 on the two opposite slits 9 are aligned, a light source 13 and an optical fiber 14 for receiving light are respectively positioned at two sides of the sample measuring piece 1, the light source 13 is adapted to be arranged with the slit 9 at the same side and aligned with the slits 10, the optical fiber 14 is adapted to be arranged with the other slit 9 at the same side and aligned with the slits 10, so that the light emitted by the light source 13 can enter a sample measuring channel through one slit 10 and then be emitted through the other slit 10 and be received by the optical fiber 14, and the optical fiber 14 is connected with a signal processor, so that the light intensity data information is analyzed and processed.

For example, a heating element is provided inside the slit 9 to enable the slit 9 to heat the sample measurement member 1. In the subsequent evaporation process, in order to increase the evaporation rate of the moisture, a slit 9 having a heating function may be selected, for example, a heating element inside the slit 9 may be a heating wire.

Wherein the negative pressure device comprises a negative pressure pump and a negative pressure channel 17; one end of the negative pressure channel 17 is communicated with the outlet of the capillary channel, and the other end is communicated with the negative pressure pump. For example, the sample measuring member 1 further includes a third columnar portion 6, the third columnar portion 6 may have a columnar structure, the third columnar portion 6 is connected to one end of the second columnar portion 5 away from the first columnar portion 4, and the capillary passage extends to an end face of the third columnar portion 6 in a direction away from the cleaning liquid accommodating groove 2; the third columnar portion 6 at least partially extends into the negative pressure channel 17, and a sealing ring 11 is arranged between the outer side wall of the third columnar portion 6 and the inner side wall of the negative pressure channel 17. During flushing, the capillary channel can be flushed by the cleaning liquid under the action of negative pressure generated by the negative pressure pump, and after flushing is finished, air flow is enabled to quickly pass through the capillary channel by utilizing the negative pressure, so that moisture in the capillary channel is quickly evaporated. Compared with the natural evaporation mode, the method can reduce the evaporation time, shorten the interval time between two experiments, and is beneficial to improving the experimental efficiency.

As shown in fig. 6, for example, the negative pressure device further includes a negative pressure tank 19 and a control valve 18; an air inlet of the negative pressure tank 19 is connected with the negative pressure channel 17, and an air outlet of the negative pressure tank 19 is connected with the negative pressure pump 20; the control valve 18 may be provided at the air inlet of the negative pressure tank 19 or on a line connected to the air inlet of the negative pressure tank 19. For example, the negative pressure device further comprises a negative pressure joint 15; one end of the negative pressure joint 15 is connected with the negative pressure channel 17, and the other end is connected with the air inlet of the negative pressure tank 19, for example, a sealing ring 11 can be arranged between the negative pressure joint 15 and the negative pressure channel 17 to improve the overall tightness. When flushing or evaporation is not needed, the control valve 18 is closed, and at the moment, the downward flowing sample compresses the air at the upstream of the control valve 18, and the compressed air can provide a certain pressure so that the liquid drops hung at the outlet of the capillary channel are not easy to drop; the negative pressure pump 20 can form a larger level of vacuum in the negative pressure tank 19 when working; when flushing or evaporation is required, the control valve 18 is opened, and the cleaning liquid or air flow is forced to pass through the capillary channel quickly by using a large-level vacuum difference, so that the cleaning and evaporation effects are improved, and the evaporation of the capillary channel can be completed in a few seconds.

As shown in fig. 4, the micro-spectrophotometer further includes a sample adding device 12, for example, the sample adding device 12 may be a dropper, and a liquid dropping port of the sample adding device 12 can extend to the capillary channel 3 through the cleaning liquid containing groove 2 so as to drop the sample to be tested into the junction of the capillary channel and the cleaning liquid containing groove 2, so that a liquid column for detection can be formed in the capillary channel by using less sample to be tested, the consumption of the sample to be tested is reduced, and the experiment cost is reduced.

It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.

Claims

1. A micro-spectrophotometer, comprising at least:

the device comprises a sample measuring piece, a sample storage device and a sample storage device, wherein a cleaning liquid containing groove and a capillary channel which are communicated are arranged on the sample measuring piece, and the cleaning liquid containing groove is positioned at the upstream of the capillary channel; wherein the part of the capillary channel for adapting to the slit is a sample measuring channel; after the liquid sample is added from the inlet of the capillary channel, the liquid sample can flow towards the outlet of the capillary channel and fill the capillary channel under the action of capillary action, and the liquid sample can keep the sample at the outlet of the capillary channel in a hanging state without dripping under the action of surface tension so as to fill the sample to be measured in the sample measuring channel;

the inlet of the negative pressure device is communicated with the outlet of the capillary channel, the negative pressure device can force the cleaning liquid in the cleaning liquid containing groove to flow through the capillary channel so as to flush the capillary channel, and the residual cleaning liquid in the capillary channel is quickly evaporated by using the fast flowing air after flushing;

the cleaning solution accommodating groove is a groove with a conical structure, the top opening of the cleaning solution accommodating groove is a cleaning solution inlet, and external cleaning solution can enter the cleaning solution accommodating groove through the cleaning solution inlet.

2. The micro-spectrophotometer as claimed in claim 1, wherein,

the sample measuring piece comprises a first columnar part and a second columnar part which are connected;

the cleaning liquid containing groove is arranged on the end face of the first columnar part far away from the second columnar part;

the capillary passage is provided inside the second cylindrical portion.

3. A micro-spectrophotometer as claimed in claim 2, wherein,

the device also comprises a slit which is in a plate-shaped structure, and a slit is arranged on the plate surface of the slit;

the periphery of the second cylindrical part is provided with two light-transmitting planes which are arranged opposite to each other, each light-transmitting plane is provided with a slit, and the slits on the two opposite slits are aligned, so that light can enter the sample measuring channel through one slit and then be emitted through the other slit.

4. A micro-spectrophotometer according to claim 2 or 3, wherein,

the negative pressure device comprises a negative pressure pump and a negative pressure channel;

one end of the negative pressure channel is communicated with the outlet of the capillary channel, and the other end of the negative pressure channel is communicated with the negative pressure pump.

5. The micro-spectrophotometer as claimed in claim 4, wherein,

the negative pressure device also comprises a negative pressure tank and a control valve;

the air inlet of the negative pressure tank is connected with the negative pressure channel, and the air outlet of the negative pressure tank is connected with the negative pressure pump;

the air inlet of the negative pressure tank is provided with the control valve.

6. The micro-spectrophotometer as claimed in claim 5, wherein,

the negative pressure device also comprises a negative pressure joint;

one end of the negative pressure connector is connected with the negative pressure channel, and the other end of the negative pressure connector is connected with the air inlet of the negative pressure tank.

7. The micro-spectrophotometer as claimed in claim 4, wherein,

the sample measuring piece further comprises a third columnar part, the third columnar part is connected with one end of the second columnar part, which is far away from the first columnar part, and the capillary channel extends to the end face of the third columnar part towards the direction far away from the cleaning liquid accommodating groove;

the third columnar part at least partially stretches into the negative pressure channel, and a sealing ring is arranged between the outer side wall of the third columnar part and the inner side wall of the negative pressure channel.

8. The micro-spectrophotometer as claimed in claim 1, wherein,

the sample adding device is characterized by further comprising a sample adding device, wherein a liquid dropping port of the sample adding device can extend to the capillary channel through the cleaning liquid containing groove so as to drop a sample to be tested into the junction of the capillary channel and the cleaning liquid containing groove.