CN117732518A - Universal detection test tube based on micro-magnetic control technology and detection method - Google Patents
Universal detection test tube based on micro-magnetic control technology and detection method Download PDFInfo
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Abstract
The invention discloses a universal detection test tube based on a micro magnetic control technology and a detection method, and relates to the technical field of detection, wherein the universal detection test tube comprises a micro magnetic control tube and a sealing transmission mechanism, the top of the micro magnetic control tube is provided with the sealing transmission mechanism, and the bottom of the micro magnetic control tube is provided with a reaction cavity; the sealing transmission mechanism comprises a top cover, the top cover is arranged at the top of the micro-magnetic control tube, the top cover is provided with a first layer of threads connected with the inner sealing groove and a second layer of threads connected with the tube orifice of the micro-magnetic control tube, the top cover is internally provided with a sealing transmission shaft, the upper end of the sealing transmission shaft is provided with a top clamping head, the lower part of the sealing transmission shaft is provided with a supporting ring with the inner diameter larger than that of the sealing transmission shaft, the lower part of the supporting ring is provided with a bottom clamping head with the inner diameter smaller than that of the supporting ring, and the sealing transmission shaft, the supporting ring, the top clamping head and the bottom clamping head are integrally arranged. The sealing function and the stirring function of the test tube are realized through the sealing transmission mechanism.
Description
Technical Field
The invention relates to the technical field of detection, in particular to a universal detection test tube based on a micro-magnetic control technology and a detection method.
Background
The magnetic bead method nucleic acid extraction principle is based on the same principle as a silica gel membrane centrifugal column, and the superparamagnetic silica nano magnetic beads are prepared by modifying and surface-modifying the surfaces of superparamagnetic nano particles by using a nano technology. The magnetic beads can be specifically identified and efficiently combined with nucleic acid molecules on a microscopic interface. The superparamagnetism of the nano magnetic microsphere coated by the silicon dioxide can separate DNA and RNA from samples of blood, animal tissues, food, pathogenic microorganisms and the like under the action of Chaotopic salt (guanidine hydrochloride, guanidine isothiocyanate and the like) and an external magnetic field, and can be applied to a plurality of fields of clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microorganism detection, food safety detection, molecular biology research and the like.
In modern medical detection, detection items of various samples (such as sputum, blood, cerebrospinal fluid, hydrothorax and ascites, body fluid, pathological sections and the like) all need to be subjected to functions of pre-separation, mixing, stirring, temperature rise and reduction, cleaning, detection and the like, but no universal test tube can realize the function of processing various different samples for different detection based on the prior art and methods.
For example, the nucleic acid amplification detection technology is a process of providing in vitro amplification conditions for nucleic acid fragments, exponentially amplifying the nucleic acid fragments in large quantities, adding fluorescent dye or fluorescent marker in the process of amplifying the nucleic acid, detecting the intensity of a fluorescent signal by adopting an optical device, and obtaining the result of amplifying the nucleic acid by processing and analyzing the fluorescent signal.
However, the nucleic acid extraction amplification test tube in the related art has a problem that the sealing is not tight, which is liable to cause pollution to sample nucleic acid, and the nucleic acid extraction to amplification test in the related art has a problem that the reflection is insufficient.
Moreover, in the related art, the nucleic acid extraction and the nucleic acid amplification are two steps, and the normal existing flow is separated, and the nucleic acid extraction test tube and the nucleic acid extraction instrument and the nucleic acid amplification test tube and the nucleic acid amplification instrument are required to be completed by two sets of reagent tube consumables.
Disclosure of Invention
The invention provides a universal test tube and a test method based on a micro-magnetic control technology, which solve the technical problems through a sealed transmission mechanism.
According to one aspect of the disclosure, a universal detection test tube based on a micro magnetic control technology is provided, and the universal detection test tube comprises a micro magnetic control tube and a sealing transmission mechanism, wherein the sealing transmission mechanism is arranged at the top of the micro magnetic control tube, and a reaction cavity is arranged at the bottom of the micro magnetic control tube;
the sealing transmission mechanism comprises a top cover, the top cover is arranged at the top of the micro-magnetic control pipe, the top cover is provided with a first layer of threads for being connected with the inner sealing groove and a second layer of threads for being connected with the pipe orifice of the micro-magnetic control pipe, a sealing transmission shaft is arranged in the top cover, the upper end of the sealing transmission shaft is provided with a top clamping head, the lower part of the sealing transmission shaft is provided with a supporting ring with the inner diameter larger than that of the sealing transmission shaft, the lower part of the supporting ring is provided with a bottom clamping head with the inner diameter smaller than that of the supporting ring, and the sealing transmission shaft, the supporting ring, the top clamping head and the bottom clamping head are integrated;
the liquid injection stirring mechanism is arranged below the sealing transmission mechanism and is used for carrying out liquid injection stirring, a preset mechanism is arranged on the outer side of the liquid injection stirring mechanism and is used for storing solid or liquid used for reaction in advance;
a sealed liquid injection mechanism and a reaction mechanism are arranged below the liquid injection stirring mechanism,
the sealing liquid injection mechanism is used for isolating the reagent in the micro-magnetic control tube from the reagent in the reaction mechanism; the reaction mechanism is used for cleaning the nucleic acid to be detected; the reaction cavity is used for carrying out the reaction of nucleic acid and reaction liquid.
In one possible implementation manner, a circular first through hole is formed in the middle of the top cover, a circular groove is formed in the first through hole, the circular groove is horizontally formed in the first through hole, the supporting ring is movably connected to the circular groove, and the supporting ring rotates in the circular groove; a circular second through hole is formed in the middle of the inner sealing groove, and threads used for being in threaded connection with the first layer of the top cover are arranged on the inner sealing groove.
In one possible implementation, the top clamping head is connected with an external motor driving mechanism, and rotates under the driving of the motor driving mechanism.
In one possible implementation manner, the liquid injection stirring mechanism comprises a liquid injection hollow rod, a top clamping groove and a double-layer stirring blade, wherein the upper end of the liquid injection hollow rod is clamped with the bottom clamping connector, the double-layer stirring blade is fixedly connected with the lower end of the liquid injection hollow rod in a spiral manner, and the double-layer stirring blade is positioned above the sealed liquid injection mechanism.
In one possible implementation manner, the preset mechanism includes a preset cover and a preset groove, a circular fourth through hole is formed in the middle of the preset cover, a cylindrical groove is formed in the middle of the preset groove, and the sealing transmission shaft penetrates through the cylindrical groove and the fourth through hole.
In one possible implementation manner, the preset groove is divided into a plurality of grooves by the partition boards in the vertical direction, a plurality of clamping grooves are formed in the bottom of the preset cover in a manner of adapting to the positions of the plurality of partition boards in the preset groove, and the bottom of the preset groove is designed into a slope opening.
In one possible implementation manner, the sealing liquid injection mechanism comprises a sealing plate and a liquid injection plate, a plurality of round holes communicated with each cavity of the reaction mechanism are formed in the liquid injection plate, a plurality of cylindrical protrusions corresponding to the round holes are formed in the lower side of the sealing plate, and the protrusions are used for sealing the round holes.
In one possible implementation manner, the reaction mechanism comprises a first communication cavity, a first isolation cavity, a cleaning cavity, a second isolation cavity and a second communication cavity which are circumferentially arranged, wherein the second communication cavity is communicated with the reaction cavity, and the reaction cavity is conical with a wide upper part and a narrow lower part.
According to an aspect of the present disclosure, there is provided a method for detecting amplification of a stirred extracted nucleic acid, the method being applied to the universal detection tube based on micromagnetic control technique, the method comprising,
Opening a cover of the micro-magnetic control tube, and adding a sample into a space between a preset mechanism and a sealed liquid injection mechanism in the micro-magnetic control tube;
the micro-magnetic control tube is sealed through the top cover, the sealing transmission shaft and the inner sealing groove;
the sealing transmission shaft is rotated manually or in a motor driving mode;
mixing the magnetic bead mixed liquid in the preset tank with the detection sample, melting paraffin at the bottom of the preset tank by heating, and flowing the liquid in the bottom of the preset tank into the middle part in the micro-magnetic control tube;
inserting the micro magnetic control test tube into a shaftless ball gear, starting heating and cracking of a detection sample, repeatedly rotating an external magnetic bead to drive the magnetic bead in the micro magnetic control tube to move, combining the cracked nucleic acid with the magnetic bead, and sequentially passing the magnetic bead through a first communication cavity, a first isolation cavity, a cleaning cavity, a second isolation cavity and a second communication cavity under the action of the external magnetic bead so as to finish cleaning;
melting paraffin at the upper part of the reaction cavity in a temperature control mode, and enabling magnetic beads carrying nucleic acid to enter the reaction cavity;
under the action of the reagent in the reaction cavity, the magnetic beads are separated from the nucleic acid, the magnetic beads reach the bottom of the reaction cavity, the nucleic acid reacts with the reaction liquid in the reaction cavity, and the detection instrument collects fluorescence from the side face of the reaction cavity.
In one possible implementation, the method further comprises, prior to performing the detection, performing the steps of,
Sequentially adding the reaction liquid, paraffin and biological reagent into a reaction cavity;
silicone oil is added into a first isolation cavity and a second isolation cavity of the reaction mechanism, and cleaning liquid is added into a cleaning cavity;
presetting solidified paraffin at the bottom of each preset groove;
and adding magnetic bead mixed liquid to paraffin at the bottom of one of the preset tanks.
Compared with the prior art, the invention has the beneficial effects that,
According to the universal detection test tube and the detection method based on the micro magnetic control technology, the reaction is carried out in the micro magnetic control tube, and the spiral sealing effect is better at the top; the sealing transmission mechanism has a sealing function and a stirring function, so that the risk of pollution of sample nucleic acid can be reduced, and the reaction can be fully carried out through stirring. The inner wall of the inner sealing groove is filled with paraffin, so that secondary sealing is performed, and the sealing effect is further improved.
Moreover, annotate liquid rabbling mechanism annotate liquid hollow rod can be convenient for add the sample liquid in the inside of little magnetic control pipe, and double-deck stirring leaf can be with the more even of reaction liquid stirring.
The number of preset grooves in the preset mechanism can be any number, and the preset grooves can be used for putting various reagents and substances in advance and can be separated before use. The sealing liquid injection hole mechanism is used for injecting various reagents and substances into the reaction tank and sealing the reaction tank; different liquids can be injected into various grooves in the reaction groove, the substances can move between different liquids under the action of the magnetic beads to achieve a desired effect, and finally required substances can move together along with the magnetic beads to mix in the reaction cavity; the interior of the reaction chamber is subjected to a series of specific reactions of the internal substances under specific conditions. The inner bottom of the preset groove adopts a slope opening design, so that the reagent can conveniently flow out of the preset groove to the interior of the micro-magnetic control tube. The function of processing a plurality of different samples for different detection can be further realized through the cooperation of the preset mechanism, the reaction mechanism and the like.
The universal detection tube based on the micro magnetic control technology combines the two steps by using a magnetic bead method, so that the operation steps and time space are saved, the cost is also saved, and the transfer of nucleic acid in a sealed environment can not cause aerosol pollution.
Drawings
FIG. 1 illustrates an anatomic view of a first view of a universal test tube based on micromagnetic control technology in accordance with an embodiment of the present disclosure.
FIG. 2 illustrates an anatomic diagram of a second view of a universal test tube based on micromagnetic control technology in accordance with an embodiment of the present disclosure.
Fig. 3 shows a partial anatomic view of a universal test tube based on micromagnetic control technology according to an embodiment of the present disclosure.
FIG. 4 is a schematic view of a sealed liquid injection mechanism, a reaction mechanism and a reaction chamber according to an embodiment of the disclosure.
Fig. 5 shows an exploded view of a universal test tube based on micromagnetic control technology according to an embodiment of the present disclosure.
Fig. 6 shows a schematic diagram of a universal test tube based on micro-magnetic control technology according to an embodiment of the disclosure.
Fig. 7 shows a schematic partial anatomic view of a preset mechanism in accordance with an embodiment of the present disclosure.
In the figure: 1. a micro-magnetic control tube; 2. sealing the transmission mechanism; 21. a top cover; 22. sealing the transmission shaft; 23. a support ring; 24. a top snap-fit; 25. a bottom clamping joint; 26. an inner seal groove; 3. a liquid injection stirring mechanism; 31. a liquid injection hollow rod; 32. a top clamping groove; 33. double-layer stirring blades; 4. a preset mechanism; 41. presetting a cover; 42. presetting a groove; 5. sealing the liquid injection mechanism; 51. a sealing plate; 52. a liquid injection plate; 6. a reaction mechanism; 61. a first communication chamber; 62. a first isolation chamber; 63. cleaning the cavity; 64. a second isolation chamber; 65. a second communication chamber; 7. a reaction chamber.
Detailed Description
Various exemplary embodiments, features and aspects of the disclosure will be described in detail below with reference to the drawings. In the drawings, like reference numbers indicate identical or functionally similar elements. Although various aspects of the embodiments are illustrated in the accompanying drawings, the drawings are not necessarily drawn to scale unless specifically indicated.
The word "exemplary" is used herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.
In addition, numerous specific details are set forth in the following detailed description in order to provide a better understanding of the present disclosure. It will be understood by those skilled in the art that the present disclosure may be practiced without some of these specific details. In some instances, methods, means, elements, and circuits well known to those skilled in the art have not been described in detail in order not to obscure the present disclosure.
Referring to fig. 1-7, the universal detection test tube based on the micro magnetic control technology disclosed by the disclosure, the universal detection test tube based on the micro magnetic control technology, wherein the micro magnetic control test tube comprises a micro magnetic control tube 1, a sealing transmission mechanism 2, a liquid injection stirring mechanism 3, a preset mechanism 4, a sealing liquid injection mechanism 5, a reaction mechanism 6 and a reaction cavity 7;
the sealing transmission mechanism 2 comprises a top cover 21, a sealing transmission shaft 22, a supporting ring 23, a top clamping connector 24, a bottom clamping connector 25 and an inner sealing groove 26, wherein the top cover 21 is provided with a first layer of threads for being connected with the inner sealing groove 26 and a second layer of threads for being connected with a pipe orifice of the micro-magnetic control pipe 1, the top clamping connector 24 is arranged at the upper end of the sealing transmission shaft 22, the supporting ring 23 with the inner diameter larger than that of the sealing transmission shaft 22 is arranged below the sealing transmission shaft 22, the sealing transmission shaft 22 with the inner diameter smaller than that of the supporting ring 23 is arranged below the supporting ring 23, and the supporting ring 23, the top clamping connector 24 and the bottom clamping connector 25 are integrated;
the liquid injection stirring mechanism 3 comprises a liquid injection hollow rod 31, a top clamping groove 32 and a double-layer stirring blade 33, wherein the upper end of the liquid injection hollow rod 31 is clamped with the bottom clamping joint 25, and the double-layer stirring blade 33 is spirally and fixedly connected with the lower end of the liquid injection hollow rod 31;
the middle of the top cover 21 is provided with a circular first through hole, the first through hole is provided with a circular groove which is horizontally arranged, the supporting ring 23 is movably connected with the circular groove, and the supporting ring 23 can rotate in the circular groove;
a circular second through hole is formed in the middle of the inner sealing groove 26, and the inner sealing groove 26 is provided with threads for being in threaded connection with the first layer of the top cover 21;
the presetting mechanism 4 is used for storing solid or liquid used for reaction in advance;
the sealing liquid injection mechanism 5 is used for isolating the reagent in the micro-magnetic control tube 1 from the reagent in the reaction mechanism 6;
the reaction mechanism 6 is used for cleaning the nucleic acid to be detected;
the reaction chamber 7 is used for carrying out the reaction of nucleic acid and a reaction solution;
after the installation is completed, the double-layer stirring blade 33 is positioned above the sealing liquid injection mechanism 5.
According to the universal detection test tube and the detection method based on the micro magnetic control technology, the reaction is carried out in the micro magnetic control tube, and the spiral sealing effect is better at the top; the sealing transmission mechanism has a sealing function and a stirring function, so that the risk of pollution of sample nucleic acid can be reduced, and the reaction can be fully carried out through stirring. The inner wall of the inner seal groove is filled with paraffin, and the function is to carry out secondary sealing. Moreover, annotate liquid rabbling mechanism annotate liquid hollow rod can be convenient for add the sample liquid in the inside of little magnetic control pipe, and double-deck stirring leaf can be with the more even of reaction liquid stirring.
The number of preset grooves in the preset mechanism can be any number, and the preset grooves are used for putting various reagents and substances in advance, so that different liquids can be isolated before the preset mechanism is used. The sealing liquid injection mechanism is used for injecting various reagents and substances into the reaction tank and sealing the reaction tank; different liquids can be injected into various grooves in the reaction groove, the substances can move between different liquids under the action of the magnetic beads to achieve a desired effect, and finally required substances can move together along with the magnetic beads to mix in the reaction cavity; the interior of the reaction chamber is subjected to a series of specific reactions of the internal substances under specific conditions. The inner bottom of the preset groove adopts a slope opening design, so that the reagent can conveniently flow out of the preset groove to the interior of the micro-magnetic control tube.
In one possible implementation, the preset mechanism 4 comprises a preset lid 41 and a preset slot 42;
the preset mechanism 4 comprises a preset cover 41 and a preset groove 42, a circular fourth through hole is formed in the middle of the preset cover 41, a cylindrical groove is formed in the middle of the preset groove 42, and the rotating shaft sealing transmission shaft 22 penetrates through the cylindrical groove and the fourth through hole;
the preset groove 42 is divided into a plurality of grooves by partition plates in the vertical direction, and a plurality of clamping grooves are formed at the bottom of the preset cover 41 and adapt to the positions of the plurality of partition plates in the preset groove 42;
the bottom of the preset groove 42 is designed into a slope opening, and the preset grooves 42 in the preset mechanism 4 can be any number, and the function is to put various reagents and solid matters in advance, so that different liquids and solid matters can be isolated before use. The inner bottom of the pre-groove 42 adopts a slope opening design, so that the reagent can conveniently flow out of the pre-groove 42 to the interior of the micro-magnetic control tube 1.
The magnetic bead method-magnetic particle separation technology-other technical fields such as chemiluminescence, chemiluminescence immunoassay technology and the like can be used, and the same luminescent detection effect is achieved by utilizing magnetic bead adsorption, wherein other grooves of the preset groove 42 are reserved for adding other reagents needing to be isolated when the chemiluminescence immunoassay method and other methods are used.
In one possible implementation, the sealing and liquid injection mechanism 5 comprises a sealing plate 51, a liquid injection plate 52 and the reaction mechanism 6 comprises a first communication cavity 61, a first isolation cavity 62, a cleaning cavity 63, a second isolation cavity 64 and a second communication cavity 65;
wherein, the liquid injection plate 52 is provided with a plurality of round holes communicated with each cavity of the reaction mechanism 6, the lower side of the sealing plate 51 is provided with a plurality of cylindrical bulges corresponding to the round holes, and the bulges are used for sealing the round holes;
the second communicating chamber 65 communicates with the reaction chamber 7;
the reaction chamber 7 has a conical shape with a wide upper part and a narrow lower part.
The seal liquid injection mechanism 5 is used for injecting various reagents and substances into the reaction tank and sealing the reaction tank.
In one possible implementation, the top snap 24 is connected to an external motor drive mechanism that rotates under the drive of the motor drive mechanism.
Alternatively, the seal drive shaft 22 is manually rotated to allow the reaction within the tube to proceed sufficiently, e.g., to accelerate the progress of the lysis reaction, so that the sample is subjected to sufficient lysis.
The invention discloses a detection method for extracting nucleic acid amplification by stirring, which is applied to a universal detection test tube based on a micromagnetic control technology, and comprises the following steps of,
Sequentially adding the reaction solution, paraffin and biological reagent into the reaction cavity 7;
silicone oil is added into a first isolation cavity 62 and a second isolation cavity 64 of the reaction mechanism 6, and cleaning liquid is added into a cleaning cavity 63; the silicone oil and the cleaning fluid are used for cleaning the magnetic beads.
Presetting solidified paraffin at the bottom of each tank of the preset tank 42;
a magnetic bead mixed liquid is added to the paraffin at the bottom of one of the preset tanks 42.
For example, the above steps are encapsulation of detection reagents for a magnetron tube.
Opening the cover of the micro-magnetic control test tube 1, and enabling the gun head of the pipetting gun to penetrate through the pipetting hollow rod 31 and the double-layer stirring blade 33 to penetrate deep into the space between the presetting mechanism 4 and the sealing pipetting mechanism 5 to add sample liquid; the micro-magnetic control tube 1 is sealed through the top cover 21, the sealing transmission shaft 22 and the inner sealing groove 26;
the seal drive shaft 22 is rotated manually or by motor drive;
mixing the magnetic bead mixed liquid in the preset tank 42 with the detection sample, melting paraffin at the bottom of the preset tank 42 by heating, and flowing the liquid in the bottom of the preset tank 42 into the middle part in the micro-magnetic control tube 1;
inserting a micro-magnetic control test tube into a shaftless ball gear, starting heating and cracking a detection sample, repeatedly rotating an external magnetic bead to drive the magnetic bead in the micro-magnetic control tube 1 to move, combining the cracked nucleic acid with the magnetic bead, and sequentially passing the magnetic bead through a first communication cavity 61, a first isolation cavity 62, a cleaning cavity 63, a second isolation cavity 64 and a second communication cavity 65 under the action of the external magnetic bead so as to finish cleaning;
for example, the cleaning chamber 63 contains cleaning liquid for cleaning the impurities on the magnetic beads.
Paraffin at the upper part of the reaction cavity 7 is melted in a temperature control mode, and magnetic beads carrying nucleic acid enter the reaction cavity 7;
under the action of the reagent in the reaction chamber 7, the magnetic beads are separated from the nucleic acid, the magnetic beads reach the bottom of the reaction chamber 7, the nucleic acid reacts with the reaction liquid in the reaction chamber 7, and the detection instrument collects fluorescence from the side surface of the reaction chamber 7.
Application example using micro magnetic control test tube,
The inner bottom of each preset groove 42 is preset with solidified paraffin in advance, magnetic bead mixed liquid is added to the paraffin in one of the preset grooves 42, other grooves can be selectively added with other liquid which needs to be isolated from the preset, and the preset cover 41 is covered to achieve the preset purpose.
When the test tube is used, the sealing transmission mechanism 2 is firstly rotated and opened, the TIP head is inserted by the pipette to absorb a sample, the TIP head penetrates through the liquid injection hollow rod 31 and the double-layer stirring blade 33 to penetrate into the space between the preset mechanism 4 and the sealing liquid injection mechanism 5, sample liquid is added into the micro-magnetic control tube, then the sealing transmission mechanism 2 is rotated and covered, the bottom clamping connector 25 is clamped with the top clamping groove 32, and then the test tube is placed on the test equipment to carry out detection reaction.
During the reaction, paraffin at the bottom of the preset groove 42 is melted by heating, as the inner bottom of the preset groove 42 adopts a slope design, paraffin and magnetic beads mixed liquid and other preset liquid flow out of the preset groove 42 to the middle part in the micro magnetic control tube 1 and the first liquid at the middle part in the micro magnetic control tube 1 are mixed, various liquids and magnetic beads are more fully mixed with the liquid at the middle part in the micro magnetic control tube 1 through the rotation of the bottom clamping connector 25, and meanwhile, under the action of an external magnet, the magnetic beads can be converged right above the first communication cavity 61, and the magnet can sequentially move along the first communication cavity 61, the first isolation cavity 62, the cleaning cavity 63 and the second isolation cavity 64 until moving right above the second communication cavity 65, and finally fall into the reaction cavity 7 through the second communication cavity 65 to react.
According to the universal detection test tube and the detection method based on the micro magnetic control technology, the reaction is carried out in the micro magnetic control tube, and the spiral sealing effect is better at the top; the sealing transmission mechanism has a sealing function and a stirring function, so that the risk of pollution of sample nucleic acid can be reduced, and the reaction can be fully carried out through stirring. The inner wall of the inner seal groove is filled with paraffin, and the function is to carry out secondary sealing.
The number of preset grooves in the preset mechanism can be any number, and the preset grooves can be used for putting various reagents and substances in advance and can be separated before use. The sealing liquid injection mechanism is used for injecting various reagents and substances into the reaction tank and sealing the reaction tank; different liquids can be injected into various grooves in the reaction groove, the substances can move between different liquids under the action of the magnetic beads to achieve a desired effect, and finally required substances can move together along with the magnetic beads to mix in the reaction cavity; the interior of the reaction chamber is subjected to a series of specific reactions of the internal substances under specific conditions. The inner bottom of the preset groove adopts a slope opening design, so that the reagent can conveniently flow out of the preset groove to the interior of the micro-magnetic control tube.
The test tube can realize the general detection of various samples (such as sputum, blood, cerebrospinal fluid, hydrothorax and ascites, body fluid, pathological sections and the like) based on a micro-magnetic control method, and the detection of corresponding nucleic acid amplification detection technology, chemiluminescent immunoassay technology and the like is only one specific implementation mode of the detection method which can be used by the test tube.
It should be noted that, for simplicity of description, the foregoing method embodiments are all described as a series of acts, but it should be understood by those skilled in the art that the present disclosure is not limited by the order of acts described, as some steps may be performed in other orders or concurrently in accordance with the present disclosure. Further, those skilled in the art will also appreciate that the embodiments described in the specification are all alternative embodiments, and that the acts and modules referred to are not necessarily required by the present disclosure.
In the foregoing embodiments, the descriptions of the embodiments are emphasized, and for parts of one embodiment that are not described in detail, reference may be made to related descriptions of other embodiments.
In the several embodiments provided in the present disclosure, it should be understood that the disclosed apparatus may be implemented in other manners. For example, the apparatus embodiments described above are merely illustrative, such as the division of the units, merely a logical function division, and there may be additional manners of dividing the actual implementation, such as multiple units or components may be combined or integrated into another system, or some features may be omitted, or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be an indirect coupling or communication connection via some interfaces, devices or units, or may be in electrical or other forms.
The foregoing has described in detail embodiments of the present disclosure, with specific examples being employed herein to illustrate the principles and implementations of the present disclosure, the above examples being provided solely to assist in understanding the methods of the present disclosure and their core ideas; meanwhile, as one of ordinary skill in the art will have variations in the detailed description and the application scope in light of the ideas of the present disclosure, the present disclosure should not be construed as being limited to the above description.
Various aspects of the present disclosure are described herein with reference to flowchart illustrations and/or block diagrams of methods, apparatus (systems) and computer program products according to embodiments of the disclosure. It will be understood that each block of the flowchart illustrations and/or block diagrams, and combinations of blocks in the flowchart illustrations and/or block diagrams, can be implemented by computer-readable program instructions.
The foregoing description of the embodiments of the present disclosure has been presented for purposes of illustration and description, and is not intended to be exhaustive or limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the technical improvements in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (10)
1. The universal detection test tube based on the micro magnetic control technology is characterized by comprising a micro magnetic control tube (1) and a sealing transmission mechanism (2), wherein the sealing transmission mechanism (2) is arranged at the top of the micro magnetic control tube (1), and a reaction cavity (7) is arranged at the bottom of the micro magnetic control tube (1);
the sealing transmission mechanism (2) comprises a top cover (21), the top cover (21) is arranged at the top of the micro-magnetic control tube (1), the top cover (21) is provided with a first layer of threads connected with an inner sealing groove (26) and a second layer of threads connected with a tube orifice of the micro-magnetic control tube (1), a sealing transmission shaft (22) is arranged in the top cover (21), the upper end of the sealing transmission shaft (22) is provided with a top clamping head (24), the lower part of the sealing transmission shaft (22) is provided with a supporting ring (23) with the inner diameter larger than that of the sealing transmission shaft (22), the lower part of the supporting ring (23) is provided with a bottom clamping head (25) with the inner diameter smaller than that of the supporting ring (23), and the sealing transmission shaft (22), the supporting ring (23), the top clamping head (24) and the bottom clamping head (25) are integrated.
A liquid injection stirring mechanism (3) is arranged below the sealing transmission mechanism (2), the liquid injection stirring mechanism (3) is used for carrying out liquid injection stirring, a preset mechanism (4) is arranged on the outer side of the liquid injection stirring mechanism (3), and the preset mechanism (4) is used for storing solid or liquid used for reaction in advance;
a sealing liquid injection mechanism (5) and a reaction mechanism (6) are arranged below the liquid injection stirring mechanism (3),
the sealing liquid injection mechanism (5) is used for isolating the reagent in the micro-magnetic control tube (1) from the reagent in the reaction mechanism (6); the reaction mechanism (6) is used for cleaning the nucleic acid to be detected; the reaction chamber (7) is used for carrying out the reaction of nucleic acid and reaction liquid.
2. The universal detection test tube based on the micromagnetic control technology according to claim 1, wherein a circular first through hole is arranged in the middle of the top cover (21), the first through hole is provided with a circular groove which is horizontally arranged, the support ring (23) is movably connected with the circular groove, and the support ring (23) rotates in the circular groove; a circular second through hole is formed in the middle of the inner sealing groove (26), and threads used for being in threaded connection with the first layer of the top cover (21) are arranged on the inner sealing groove (26).
3. The universal test tube based on micro-magnetic control technology according to claim 1, wherein the top clamping head (24) is connected with an external motor driving mechanism and is driven to rotate by the motor driving mechanism.
4. The micro-magnetic control technology-based universal detection test tube according to claim 1, wherein the liquid injection stirring mechanism (3) comprises a liquid injection hollow rod (31), a top clamping groove (32) and a double-layer stirring blade (33), the upper end of the liquid injection hollow rod (31) is clamped with the bottom clamping head (25), the double-layer stirring blade (33) is fixedly connected with the lower end of the liquid injection hollow rod (31) in a spiral manner, and the double-layer stirring blade (33) is located above the sealing liquid injection mechanism (5).
5. The micro-magnetic control technology-based universal test tube according to claim 1, wherein the presetting mechanism (4) comprises a presetting cover (41) and a presetting groove (42), a round fourth through hole is formed in the middle of the presetting cover (41), a cylindrical groove is formed in the middle of the presetting groove (42), and the sealing transmission shaft (22) penetrates through the cylindrical groove and the fourth through hole.
6. The micro-magnetic control technology-based universal test tube according to claim 5, wherein the preset groove (42) is divided into a plurality of grooves by partition plates in the vertical direction, a plurality of clamping grooves are formed in the bottom of the preset cover (41) in a manner of adapting to the positions of the plurality of partition plates in the preset groove (42), and a slope opening is formed in the bottom of the preset groove (42).
7. The micro-magnetic control technology-based universal detection test tube according to claim 1, wherein the sealing liquid injection mechanism (5) comprises a sealing plate (51) and a liquid injection plate (52), a plurality of round holes communicated with the cavities of the reaction mechanism (6) are formed in the liquid injection plate (52), a plurality of cylindrical protrusions corresponding to the round holes are formed in the lower side of the sealing plate (51), and the protrusions are used for sealing the round holes.
8. The micro-magnetic control technology-based universal detection test tube according to claim 1, wherein the reaction mechanism (6) comprises a first communication cavity (61), a first isolation cavity (62), a cleaning cavity (63), a second isolation cavity (64) and a second communication cavity (65) which are circumferentially arranged, the second communication cavity (65) is communicated with the reaction cavity (7), and the reaction cavity (7) is conical with a wide upper part and a narrow lower part.
9. A detection method for extracting nucleic acid amplification by stirring is characterized in that the method is applied to the universal detection test tube based on the micromagnetic control technology according to any one of claims 1 to 8, and comprises the following steps,
Opening the cover of the micro magnetic control tube (1) and adding a sample into a space between a preset mechanism (4) and a sealed liquid injection mechanism (5) in the micro magnetic control tube (1);
the micro-magnetic control tube (1) is sealed through the top cover (21), the sealing transmission shaft (22) and the inner sealing groove (26);
-rotating the sealing transmission shaft (22) manually or by motor drive;
mixing the magnetic bead mixed liquid in the preset tank (42) with a detection sample, melting paraffin at the bottom of the preset tank (42) by heating, and allowing the liquid in the bottom of the preset tank (42) to flow into the middle part in the micro-magnetic control tube (1);
inserting the micro magnetic control test tube into a shaftless ball gear, starting heating and cracking of a detection sample, repeatedly rotating an external magnetic bead to drive the magnetic bead in the micro magnetic control tube (1) to move, combining the cracked nucleic acid with the magnetic bead, and sequentially passing the magnetic bead through a first communication cavity (61), a first isolation cavity (62), a cleaning cavity (63), a second isolation cavity (64) and a second communication cavity (65) under the action of the external magnetic bead so as to finish cleaning;
paraffin at the upper part of the reaction cavity (7) is melted in a temperature control mode, and magnetic beads carrying nucleic acid enter the reaction cavity (7);
under the action of reagents in the reaction cavity (7), magnetic beads are separated from nucleic acid, the magnetic beads reach the bottom of the reaction cavity (7), the nucleic acid reacts with reaction liquid in the reaction cavity (7), and a detection instrument collects fluorescence from the side face of the reaction cavity (7).
10. The method according to claim 9, further comprising, before the detection, the steps of
Sequentially adding the reaction solution, paraffin and biological reagent into a reaction cavity (7);
silicone oil is added into a first isolation cavity (62) and a second isolation cavity (64) of the reaction mechanism (6), and cleaning liquid is added into a cleaning cavity (63);
presetting solidified paraffin at the bottom of each groove of the preset groove (42);
and adding magnetic bead mixed liquid to paraffin at the bottom of one of the preset grooves (42).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311754018.6A CN117732518A (en) | 2023-12-20 | 2023-12-20 | Universal detection test tube based on micro-magnetic control technology and detection method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311754018.6A CN117732518A (en) | 2023-12-20 | 2023-12-20 | Universal detection test tube based on micro-magnetic control technology and detection method |
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| CN117732518A true CN117732518A (en) | 2024-03-22 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109706056A (en) * | 2019-01-14 | 2019-05-03 | 北京君立康博科技有限公司 | Nucleic acid-extracting apparatus |
| KR102159936B1 (en) * | 2019-05-21 | 2020-09-25 | 주식회사 골든바이오텍 | Specimen vial container configured to mix plurality of liquid by pressing cap |
| CN114085743A (en) * | 2021-11-11 | 2022-02-25 | 杭州天微基因科技有限公司 | Full-automatic nucleic acid processing amplification detection method and magnetic control detection test tube |
| CN114752489A (en) * | 2022-06-16 | 2022-07-15 | 上海简逸生物科技有限公司 | Nucleic acid rapid detection instrument and kit |
| CN116557681A (en) * | 2023-06-09 | 2023-08-08 | 宁夏大学 | Pipeline detection device of self-adaptation pipe diameter based on micromagnetic detection |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109706056A (en) * | 2019-01-14 | 2019-05-03 | 北京君立康博科技有限公司 | Nucleic acid-extracting apparatus |
| KR102159936B1 (en) * | 2019-05-21 | 2020-09-25 | 주식회사 골든바이오텍 | Specimen vial container configured to mix plurality of liquid by pressing cap |
| CN114085743A (en) * | 2021-11-11 | 2022-02-25 | 杭州天微基因科技有限公司 | Full-automatic nucleic acid processing amplification detection method and magnetic control detection test tube |
| CN114752489A (en) * | 2022-06-16 | 2022-07-15 | 上海简逸生物科技有限公司 | Nucleic acid rapid detection instrument and kit |
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