CN117717507A - Hair-blacking and hair-loss-preventing essence containing plant extracts and preparation method thereof - Google Patents

Hair-blacking and hair-loss-preventing essence containing plant extracts and preparation method thereof Download PDF

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CN117717507A
CN117717507A CN202311741614.0A CN202311741614A CN117717507A CN 117717507 A CN117717507 A CN 117717507A CN 202311741614 A CN202311741614 A CN 202311741614A CN 117717507 A CN117717507 A CN 117717507A
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hair
extract
blacking
lactobacillus
plant extract
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朱永闯
林朝栋
叶海敏
邓燕娇
王瑜
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Guangzhou Youke Biotechnology Co ltd
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Guangzhou Youke Biotechnology Co ltd
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    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract

The invention provides a hair-blacking and hair-loss-preventing essence containing a plant extract, which comprises the following components in parts by mass: 3-6% of composite fermentation liquid, 0.6-1.0% of geranium extract, 0.1-0.4% of glossy privet fruit extract, 0.4-0.9% of chaste tree leaf algae extract, 0.3-0.6% of lemon peel extract, 0.6-1.1% of ganoderma atrum extract, 0.2-0.5% of myrobalan meat extract, 0.7-1.5% of sparrow flower extract, 0.1-0.5% of solubilizer, 1-3% of humectant, 0.1-0.5% of antioxidant, 0.7-1.3% of regulator and 0.3-0.7% of preservative, and the balance of solvent. The essence of the invention has very good effect on seborrheic alopecia symptoms caused by male androgenesis, can penetrate into hair follicles, repair and condition melanin forming passages, and simultaneously realize hair blacking effect.

Description

Hair-blacking and hair-loss-preventing essence containing plant extracts and preparation method thereof
Technical Field
The invention belongs to the technical field of shampoos, and particularly relates to a hair-blacking and hair-loss-preventing essence containing plant extracts and a preparation method thereof.
Background
It is counted that over 2.5 hundred million people worldwide suffer from alopecia, which is not only an appearance problem but also a psychological health problem nowadays, and many people feel spelt because of sparse hair and posterior hairline, and have great impact on their self-confidence. There are many causes of alopecia, such as genetic, age, immune, endocrine dyscrasia, stress, etc., which cause alopecia. Wherein, the incidence of androgenetic alopecia (AGA) accounts for more than 90 percent of alopecia, is the first cause of alopecia at present, and has the characteristics of repeated attack and difficult healing. One of the reasons for the occurrence of AGA is that overexpression of the Androgen Receptor (AR) makes scalp hair follicles abnormally sensitive to androgens. At present, western medicine components are mainly adopted for the AR receptor antagonist, but the side effects of the western medicine components are larger. In the field of scalp care products, research and development of essence products specifically aiming at AR antagonism are increasingly paid attention to, but related products on the market are still fewer at present, and the effect is not ideal.
Disclosure of Invention
The invention aims to provide hair-blacking and hair-loss-preventing essence containing plant extracts and a preparation method thereof, wherein the essence has a very good effect on seborrheic hair loss symptoms caused by male androgenesis, can penetrate into hair follicles, can repair and condition melanin formation passages, and simultaneously realizes hair-blacking effects.
In order to achieve the above object, the present invention provides the following technical solutions:
the hair-blacking and hair-loss-preventing essence containing the plant extracts comprises the following components in parts by weight: 3-6% of composite fermentation liquor, 0.6-1.0% of geranium extract, 0.1-0.4% of glossy privet fruit extract, 0.4-0.9% of aphyllum dulcis extract, 0.3-0.6% of lemon peel extract, 0.6-1.1% of ganoderma atrum extract, 0.2-0.5% of myrobalan meat extract, 0.7-1.5% of sparrow flower extract, 0.1-0.5% of solubilizer, 1-3% of humectant, 0.1-0.5% of antioxidant, 0.7-1.3% of regulator, 0.3-0.7% of preservative and the balance of solvent;
the composite fermentation broth is obtained by fermenting Lactobacillus acidophilus with a preservation number of AS1.1854, bifidobacterium longum with a preservation number of CICC24632, lactobacillus reuteri with a preservation number of CICC6124 and Lactobacillus situm with a preservation number of JCM 2762.
Lactobacillus acidophilus, accession number AS1.1854, source history: the institute of microbiology of the national academy of sciences.
Bifidobacterium longum, deposit No. cic 24632, source history: chinese food fermentation industry institute.
Lactobacillus reuteri, accession number cic 6124, source history: china center for Industrial microorganism culture Collection.
Lactobacillus glutamicum Lactobacillus farraginis, accession number JCM2762, source history: tanasawa.
Further, the preparation method of the composite fermentation broth comprises the following steps:
(1) Washing rice, soaking in distilled water 3-5 times of the rice, soaking for 1-2 hr, and filtering to obtain filtrate A; pulverizing fructus Gleditsiae Abnormalis to 100 mesh, soaking for 1-2 hr, boiling for 15-20min, and filtering to obtain filtrate B.
(2) Filtrate A and filtrate B were mixed in a volume ratio of 1:1 mixing, adding lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus chaff, anaerobic fermenting at 36 ℃ for 8-10 days, sterilizing at 70 ℃ for 30min to obtain a composite fermentation broth.
Dai nationality in Yunnan carries out natural fermentation on rice washing water for washing hair, has the effects of removing dandruff, moisturizing hair, brightening hair, blackening hair and the like, so that hair blackening products using fermented rice also appear on the market, but no effect related to hair loss prevention is found. Researchers have tried to use rice fermentation broth for androgenic alopecia during the development process, but the effect is not ideal. The rice and the Chinese honeylocust fruit are fermented by using the specific four strains at the same time, and the obtained composite fermentation liquor is compounded with various natural plant extracts, so that the composite fermentation liquor has remarkable effect on improving the symptoms of seborrheic alopecia caused by male androgenetic sex. The rice and the Chinese honeylocust fruit are obtained after four strains are fermented, have antagonistic effect on AR, and are compounded with specific plant extracts to play a role in synergy. Meanwhile, the selected multiple plant extracts are compounded with the fermentation liquor, so that the hair follicle environment can be improved, nutrition can be supplemented for hair follicles, hair roots can be nourished, and further the hair blacking effect can be achieved.
Further, the ratio of the total mass of lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus chaff to the total volume of filtrate A and filtrate B is 6-10g/L.
Further, the regulator comprises lauric acid monoglyceride, caprylic acid monoglyceride, citric acid monoglyceride, polyglycerol fatty acid ester, sucrose palmitate.
Further, the regulator is prepared from the following components in percentage by mass: 2-5:0.3-0.6 of lauric acid monoglyceride, sucrose palmitate and polyglycerol fatty acid ester.
Lauric acid monoglyceride, CAS:142-18-7; polyglycerin fatty acid ester: CAS:67784-82-1; sucrose palmitate: CAS:26446-38-8, monoester content 85%, hubei Langbowan biological medicine Co., ltd.
The regulator is added into the system to promote the compatibility between a plurality of plant extracts in the system, fermentation products and a plurality of auxiliary agents, thereby being beneficial to better playing effect of active components. The inventors have also unexpectedly found that the type of conditioning agent affects the normal temperature storage stability of the essence at the same time, when the conditioning agent is in mass ratio of 1:2-5: when lauric acid monoglyceride, sucrose palmitate and polyglycerol fatty acid ester are used in the amount of 0.3-0.6, the storage time of the essence at normal temperature is longer, but the storage time at high temperature is still not ideal, and layering, turbidity or precipitation are easy to occur.
Further, the mass ratio of the inoculum sizes of lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus chaff is 5-8:2-4:1:0.3-0.8.
In order to obtain the optimal anti-hair loss active component, four strains of lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus oryzanol are selected to ferment rice water and Chinese honeylocust fruit water. It was also found that when the mass ratio of the inoculation of the four strains was 5-8:2-4:1: and when the content is 0.3-0.8, the preservation time of the essence at high temperature is also improved. It is hypothesized that the types and activities of metabolites and enzymes produced by different species during fermentation may be different, and these metabolites and enzymes may interact with chemical components in plant extracts to form complexes or change their structures, thereby affecting the thermal stability of the system, and at the same time, the species used in the present invention may produce substances having antioxidant or thermal degradation resistant activities, or substances having protective effects, reducing deterioration of plant extracts at high temperatures. In addition, the microbial metabolites present in the bacterial fermentation broth may have a certain thermal stability, which is capable of stably existing and protecting the plant extract at high temperature.
Further, the solubilizing agent is PEG-40 hydrogenated castor oil. Purchased from jinan rong chemical industry limited.
Further, the antioxidant is tocopherol.
Further, the humectant is selected from one or more of butanediol, propylene glycol, lauryl alcohol, glycerol cocoate.
Further, the preservative is selected from phenoxyethanol or ethylhexyl glycerol.
Further, the solvent is a mixture of water and ethanol; the volume ratio of the water to the ethanol is 1:1.5-2.
The invention also provides a preparation method of the essence, which comprises the following steps of dissolving the solubilizer, the humectant, the regulator and the preservative in the solvent, and heating to 75-80 ℃; cooling the system to 45-50deg.C, adding the rest components, and stirring for 40-50min; finally cooling to normal temperature.
The raw materials used in the invention are all commercially available.
Compared with the prior art, the invention has the advantages that:
1. the invention provides a hair-blacking and hair-loss-preventing essence containing plant extracts and a preparation method thereof, wherein the essence has a very good effect on seborrheic hair loss symptoms caused by male androgenesis, can penetrate into hair follicles, can repair and regulate melanin formation passages, and simultaneously realizes hair-blacking effects.
2. The rice and the Chinese honeylocust fruit are fermented by four strains simultaneously, and the obtained composite fermentation liquor is compounded with various natural plant extracts, so that the composite fermentation liquor has remarkable effect of improving the symptoms of seborrheic alopecia caused by male androgenetic sex.
3. The selected multiple plant extracts are compounded with the fermentation liquor, so that the hair follicle environment can be improved, nutrition is supplemented, hair roots are nourished, melanin is produced, and further the effect of blackening hair is realized.
4. The regulator is added in the system of the invention, which is helpful for better exertion of the active components. When the mass ratio of the regulator is 1:2-5:0.3-0.6 of lauric acid monoglyceride, sucrose palmitate and polyglycerol fatty acid ester, the essence has longer storage time at normal temperature.
5. According to the invention, four strains of lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus oryzanol are selected for fermenting rice water and saponin water, so that active components are obtained, and meanwhile, when the mass ratio of the inoculation amount of the four strains is 5-8:2-4:1: and at 0.3-0.8, the preservation time of the essence at high temperature is also improved.
Drawings
Fig. 1 is a schematic view of hair loss preventing and hair blacking effects of a subject using the essence prepared in example 1; wherein, the left image is before use, and the right image is after use.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The embodiment provides a hair-blacking and hair-loss-preventing essence containing plant extracts, which comprises the following components in parts by mass: 5% of composite fermentation liquor, 0.8% of geranium extract, 0.3% of glossy privet fruit extract, 0.7% of chaste tree leaf algae extract, 0.5% of lime peel extract, 0.9% of ganoderma atrum extract, 0.3% of myrobalan meat extract, 1.1% of sparrow flower extract, 0.3% of solubilizer, 2% of humectant, 0.4% of antioxidant, 1.2% of regulator, 0.5% of preservative and the balance of solvent;
the composite fermentation broth is obtained by fermenting Lactobacillus acidophilus with a preservation number of AS1.1854, bifidobacterium longum with a preservation number of CICC24632, lactobacillus reuteri with a preservation number of CICC6124 and Lactobacillus situm with a preservation number of JCM 2762.
The preparation method of the composite fermentation broth comprises the following steps:
(1) Washing rice, soaking in distilled water of 4 times mass for 1.5 hr, and filtering to obtain filtrate A; pulverizing fructus Gleditsiae Abnormalis to 100 mesh, soaking for 1.5 hr, boiling for 20min, and filtering to obtain filtrate B.
(2) Filtrate A and filtrate B were mixed in a volume ratio of 1:1, adding lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus chaff, anaerobic fermenting at 36 ℃ for 10 days, and sterilizing at 70 ℃ for 30min to obtain a composite fermentation liquid.
The ratio of the total mass of lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus oryzanol to the total volume of each filtrate A and filtrate B is 8g/L.
The mass ratio of the inoculum sizes of lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus chaff is 6:3:1:0.5.
the regulator is prepared from the following components in percentage by mass: 4:0.5 lauric acid monoglyceride, sucrose palmitate and polyglycerol fatty acid ester.
The solubilizer is PEG-40 hydrogenated castor oil.
The antioxidant is tocopherol.
The humectant is propylene glycol.
The preservative is phenoxyethanol.
The solvent is a mixture of water and ethanol in a volume ratio of 1:1.7.
The embodiment also provides a preparation method of the essence, comprising the following steps of dissolving a solubilizer, a humectant, a regulator and a preservative in a solvent, and heating to 77 ℃; cooling the system to 47 ℃, continuously adding the rest components and stirring for 50min; finally cooling to normal temperature.
Example 2
The differences between this embodiment and embodiment 1 are: the hair-blacking and hair-loss-preventing essence containing the plant extracts comprises the following components in parts by weight: 3% of composite fermentation liquor, 1.0% of geranium extract, 0.1% of glossy privet fruit extract, 0.9% of chaste tree leaf algae extract, 0.3% of lime peel extract, 1.1% of ganoderma atrum extract, 0.2% of myrobalan meat extract, 1% of delphinium extract, 0.5% of solubilizer, 1% of humectant, 0.5% of antioxidant, 1% of regulator, 0.7% of preservative and the balance of solvent.
Comparative example 1
The difference between this comparative example and example 1 is: the hair-blacking and hair-loss-preventing essence containing the plant extracts comprises the following components in parts by weight: 2% of composite fermentation liquor, 0.4% of geranium extract, 0.7% of glossy privet fruit extract, 0.3% of chaste tree leaf algae extract, 1.3% of lime peel extract, 0.2% of ganoderma atrum extract, 0.9% of myrobalan meat extract, 0.4% of delphinium extract, 0.3% of solubilizer, 0.2% of humectant, 0.7% of antioxidant, 0.5% of regulator, 0.4% of preservative and the balance of solvent.
Comparative example 2
The difference between this comparative example and example 1 is: the composite fermentation broth is obtained by fermenting lactobacillus acidophilus Lactobacillus acidophilus (source history: separation of major laboratories of the university of northeast agriculture (1.0204)/(the university of dairy science) with a preservation number of CICC 20244, bifidobacterium longum (source history: beijing Kawanda Biotechnology products limited company) with a preservation number of CICC 6068, clostridium acetobutylicum (source history: the institute of China academy of sciences microbiology) with a preservation number of CGMCC 1.5074 and bifidobacterium animalis Bifidobacterium animalis (source history: the institute of Gansu academy of sciences of biology) with a preservation number of ZBM33 with a mass ratio of 1:1:1:1.
Comparative example 3
The difference between this comparative example and example 1 is: the preparation method of the composite fermentation broth comprises the following steps:
(1) Washing rice, soaking in distilled water 4 times of the rice, soaking for 2 hours, and filtering to obtain filtrate A;
(2) Adding lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus chaff into the filtrate A, performing anaerobic fermentation at 36 ℃ for 10 days, and sterilizing at 70 ℃ for 30min to obtain a composite fermentation broth.
Comparative example 4
The difference between this comparative example and example 1 is: the preparation method of the composite fermentation broth comprises the following steps:
(1) Pulverizing fructus Gleditsiae Abnormalis to 100 mesh, soaking for 2 hr, boiling for 20min, and filtering to obtain filtrate B.
(2) Adding lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus chaff into the filtrate B, performing anaerobic fermentation at 36 ℃ for 10 days, and sterilizing at 70 ℃ for 30min to obtain a composite fermentation broth.
Comparative example 5
The difference between this comparative example and example 1 is: the regulator is prepared from the following components in percentage by mass: 1: tween 80, span 60 and fatty alcohol polyoxyethylene ether AEO-9.
Comparative example 6
The difference between this comparative example and example 1 is: the mass ratio of the inoculating amount of lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus oryzanol is 1:1:1:1.
performance testing
1. LNCaP cells contained human post AR cell lysates and AR proteins were quantitatively analyzed using standard western blot assay methods. The specific operation is as follows: the LNCaP cells were incubated with the extracts prepared in examples and comparative examples of the present invention (the extracts account for 10wt% of the incubation system) for 48 hours, respectively, a blank control was not added with any product, and the percentage of residual AR contained in the lysate after incubation (amount of AR protein after incubation/amount of AR protein before incubation×100%) was measured. The results are shown in Table 1.
Table 1 measurement results
As can be seen from Table 1, the product of the present invention has a significant antagonism against AR.
2. The subjects tested for skin irritation by human body spot pasting are selected, the number of the test subjects is 60, 50 men and 10 women are all diagnosed with male hormone alopecia symptoms after the ages of 20-60 years. The products of examples and comparative examples were used for the test of skin irritation on body patches. The specific operation is as follows: the products of the examples and comparative examples (25. Mu.L) were respectively dropped onto a filter paper sheet attached to a plaque tester and placed in the plaque tester; applying the patch test on the forearm curved side of the subject with a non-irritating adhesive tape for applying on skin uniformly by palm light pressure for 24 hr; after removing the spot tester for 30min, the skin reaction is observed after the indentation disappears. If the result is negative, the people of each grade are counted after 24 hours and 48 hours of the patch test are observed again. The identification criteria are shown in Table 2 and the results are shown in Table 3.
Table 2 identification criteria
Grade Authentication criteria (symbol)
0 Negative reaction: no irritation and erythema
1 Can be reacted: mild erythema ±
2 Weak positive reaction: erythema (red spot) +
3 Strong positive reaction: erythema, papule and blister ++
4 Very positive reaction: severe edema, large foam +++
TABLE 3 skin irritation test results for human body Patch
30min 24h 48h
Example 1 0 0 0
Example 2 0 0 0
Comparative example 1 0 0 0
Comparative example 2 0 0 3
Comparative example 3 0 0 0
Comparative example 4 0 0 0
Comparative example 5 0 0 1
Comparative example 6 0 0 1
2. Selecting the subjects passing the human body spot skin irritation test, wherein the number of the subjects is 60, 50 men and 10 women are aged 20-60 years, and the subjects have alopecia symptoms, wherein 10 men are white hair subjects aged 50-60 years, and the trial method is as follows: the subject used the product of example 1 directly, once every two days, for three months. The using method comprises the following steps: after the hair was wet, 10ml of the product of example 1 was added to the hair, the hair was gently rubbed with a finger for 5 minutes, and then rinsed clean with clear water.
After the product is used for three months, 52 people can obviously relieve the symptoms of alopecia (hair loss is less than 50 pieces per day), and 8 people still have little alopecia (hair loss is not less than 50 pieces per day); 38 hair grows from the original hair loss part, 17 vellus hairs grow from the original hair loss part, and 5 no hair grows from the original hair loss part. Subjects with white hair all had varying degrees of blackening of their white hair.
4. Taking essence of the embodiment and the comparative example, respectively pouring the essence into test tubes in equal amounts, sealing, respectively placing the test tubes in an environment of-5 ℃ and 25 ℃ and 40 ℃ for one month and 10 cycles, and observing the appearance, wherein 1 cycle is respectively placed in the environment of-5 and 40 ℃ for 24 hours. The appearance of the product was observed. The results are shown in Table 4.
Table 4 stability test results
While the foregoing is directed to the preferred embodiments of the present invention, it will be appreciated by those skilled in the art that various modifications and adaptations can be made without departing from the principles of the present invention, and such modifications and adaptations are intended to be comprehended within the scope of the present invention.

Claims (10)

1. The hair-blacking and hair-loss-preventing essence containing the plant extracts is characterized by comprising the following components in parts by mass: 3-6% of composite fermentation liquor, 0.6-1.0% of geranium extract, 0.1-0.4% of glossy privet fruit extract, 0.4-0.9% of chaste tree extract, 0.3-0.6% of citrus peel extract, 0.6-1.1% of ganoderma atrum extract, 0.2-0.5% of myrobalan meat extract, 0.7-1.5% of sparrow flower extract, 0.1-0.5% of solubilizer, 1-3% of humectant, 0.1-0.5% of antioxidant, 0.7-1.3% of regulator and 0.3-0.7% of preservative, and the balance of solvent;
the composite fermentation broth is obtained by fermenting Lactobacillus acidophilus with a preservation number of AS1.1854, bifidobacterium longum with a preservation number of CICC24632, lactobacillus reuteri with a preservation number of CICC6124 and Lactobacillus situm with a preservation number of JCM 2762.
2. The plant extract-containing hair blacking and hair loss prevention essence according to claim 1, wherein the mass ratio of lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus oryzanol is 5-8:2-4:1:0.3-0.8.
3. The plant extract-containing hair blacking and hair loss prevention essence according to claim 2, wherein the preparation method of the composite fermentation broth comprises the following steps:
(1) Washing rice, soaking in distilled water 3-5 times of the rice, soaking for 1-2 hr, and filtering to obtain filtrate A; pulverizing fructus Gleditsiae Abnormalis to 100 mesh, soaking for 1-2 hr, boiling for 15-20min, and filtering to obtain filtrate B.
(2) Filtrate A and filtrate B were mixed in a volume ratio of 1:1 mixing, adding lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri and lactobacillus oryzanol, and anaerobic fermenting at 36 ℃ for 8-10 days; sterilizing at 70deg.C for 30min to obtain composite fermentation broth.
4. The plant extract-containing hair blacking and hair loss preventing essence according to claim 1, wherein the regulator comprises one or more of lauric acid monoglyceride, caprylic acid monoglyceride, citric acid monoglyceride, polyglycerin fatty acid ester, sucrose palmitate.
5. The plant extract-containing hair blacking and hair loss preventing essence according to claim 4, wherein the regulator is 1 in mass ratio: 2-5:0.3-0.6 of lauric acid monoglyceride, sucrose palmitate and polyglycerol fatty acid ester.
6. The plant extract-containing hair blacking and hair loss prevention essence according to claim 1, wherein the solubilizing agent is PEG-40 hydrogenated castor oil; the antioxidant is tocopherol.
7. The plant extract-containing hair blacking and hair loss prevention essence according to claim 1, wherein the humectant is selected from one or more of butylene glycol, propylene glycol, lauryl alcohol, glycerin cocoate.
8. The plant extract-containing hair blacking and hair loss preventing essence according to claim 1, wherein the preservative is selected from phenoxyethanol or ethylhexyl glycerol.
9. The plant extract-containing hair blacking and hair loss preventing essence according to claim 1, wherein the solvent is a mixture of water and ethanol.
10. The method for preparing the plant extract-containing hair blacking and hair loss prevention essence according to any one of claims 1 to 9, characterized by comprising the steps of: dissolving solubilizer, humectant, regulator and antiseptic in solvent, and heating to 75-80deg.C; cooling the system to 45-50deg.C, adding the rest components, and stirring for 40-50min; and finally cooling to normal temperature to obtain the essence.
CN202311741614.0A 2023-12-18 2023-12-18 Hair-blacking and hair-loss-preventing essence containing plant extracts and preparation method thereof Pending CN117717507A (en)

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KR20140076240A (en) * 2012-12-12 2014-06-20 주식회사 제닉 Cosmetic composition for improving atopy dermatitis containing fermented herb extract
CN105997836A (en) * 2016-05-13 2016-10-12 江燕馨 Ferment liquid shampoo and preparation method thereof
CN110292553A (en) * 2019-08-01 2019-10-01 天蓝水清健康产业有限公司 A kind of application of ginger ferment in preparation Hair-loss-preventingshampoo shampoo
CN110638697A (en) * 2019-11-01 2020-01-03 上海浅娇生物科技有限公司 Preparation and application of ganoderma lucidum lactobacillus fermentation extract
CN111053711A (en) * 2019-12-31 2020-04-24 山东凤凰生物有限公司 Ganoderma lucidum probiotic compound fermentation product, preparation method and application thereof
KR102258858B1 (en) * 2020-02-28 2021-06-04 (주)나우코스 Shampoo composition for promoting hair growth comprising fermented extract of maca and mistletoe and Polygonum multiflorum
CN112402358A (en) * 2020-12-11 2021-02-26 桂林长发小寨生物科技有限公司 Probiotic plant fermentation product with hair loss prevention function and preparation method thereof
CN112535656A (en) * 2020-12-11 2021-03-23 桂林长发小寨生物科技有限公司 Probiotic plant fermentation product with hair blacking function and preparation method
CN112494407A (en) * 2020-12-29 2021-03-16 广西道之良品生物科技有限公司 Composition for preventing hair loss and nourishing hair and preparation method thereof
CN115778882A (en) * 2023-02-09 2023-03-14 北京普世安生物科技有限公司 Hair-growing essence and preparation method thereof

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