CN117705533A - Neutral gum-free rapid curing cover glass for pathological sections - Google Patents
Neutral gum-free rapid curing cover glass for pathological sections Download PDFInfo
- Publication number
- CN117705533A CN117705533A CN202311796348.1A CN202311796348A CN117705533A CN 117705533 A CN117705533 A CN 117705533A CN 202311796348 A CN202311796348 A CN 202311796348A CN 117705533 A CN117705533 A CN 117705533A
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- China
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- neutral gum
- cover glass
- piece
- glass
- rapid curing
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- 230000007935 neutral effect Effects 0.000 title claims abstract description 48
- 239000006059 cover glass Substances 0.000 title claims abstract description 39
- 230000001575 pathological effect Effects 0.000 title claims abstract description 30
- 230000003287 optical effect Effects 0.000 claims abstract description 8
- 239000011521 glass Substances 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 11
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 10
- 239000011248 coating agent Substances 0.000 claims description 9
- 238000000576 coating method Methods 0.000 claims description 9
- 239000008096 xylene Substances 0.000 claims description 9
- 239000002952 polymeric resin Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 229920003002 synthetic resin Polymers 0.000 claims description 4
- 238000003698 laser cutting Methods 0.000 claims description 3
- 238000009461 vacuum packaging Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 abstract description 51
- 239000002390 adhesive tape Substances 0.000 abstract description 11
- 238000004043 dyeing Methods 0.000 abstract description 11
- 230000007170 pathology Effects 0.000 abstract description 6
- 239000003849 aromatic solvent Substances 0.000 abstract description 5
- 230000006378 damage Effects 0.000 abstract description 5
- 230000001066 destructive effect Effects 0.000 abstract description 5
- 238000001035 drying Methods 0.000 abstract description 5
- 230000007774 longterm Effects 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 239000007789 gas Substances 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 206010028813 Nausea Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 208000002173 dizziness Diseases 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 230000008693 nausea Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000779819 Syncarpia glomulifera Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005304 optical glass Substances 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 239000001739 pinus spp. Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229940036248 turpentine Drugs 0.000 description 1
- 150000003738 xylenes Chemical class 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02B—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO BUILDINGS, e.g. HOUSING, HOUSE APPLIANCES OR RELATED END-USER APPLICATIONS
- Y02B80/00—Architectural or constructional elements improving the thermal performance of buildings
- Y02B80/22—Glazing, e.g. vaccum glazing
Landscapes
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the technical field of sealing sheets after pathological section dyeing, and discloses a neutral gum-free rapid curing cover glass for pathological sections. The neutral gum-free rapid curing cover glass for pathological sections does not need to be added with neutral gum and does not contain aromatic solvents; the neutral gum free quick-curing cover glass sealing piece can be quickly cured and dried after 10 minutes, so that a doctor can read a piece and a patient can check out the piece by means of the slicing, the full-digital scanning and timely archiving and storage of the piece are facilitated, the space for drying the piece in a department is saved, the harm to workers and surrounding environment caused by harmful gas in a long period of time is avoided, the neutral gum free quick-curing cover glass sealing piece has more excellent transparency and optical performance than an imported sealing piece machine adhesive tape, a destructive structure cannot be formed on the piece for a long period of time, and the neutral gum free quick-curing cover glass is applicable to various existing sealing piece machines and manual sealing piece modes in a pathology department.
Description
Technical Field
The invention relates to the technical field of sealing sheets after pathological section dyeing, in particular to a neutral gum-free rapid curing cover glass for pathological sections.
Background
The sealing sheet after pathological section dyeing is the most important ring in clinical pathological diagnosis, whether HE dyeing, bus dyeing, swiss dyeing or immunohistochemical dyeing, in order to facilitate the observation and long-term preservation of the section under a microscope, the dyed section must be thoroughly dehydrated by alcohol, transparent by dimethylbenzene, and then the neutral gum is dripped to cover by a cover glass;
the sealing sheet after pathological section dyeing is the last procedure of the standard step of the conventional tissue section, and the sealing sheet process is to seal the dyed section by using a sealing agent, namely, to bond the cover glass and the glass slide together by using the sealing agent. The measurement of the complete dryness of the sections is that there should be no sliding between the coverslip and the slide when rubbed with two fingers. The slices can be erected after that and stacked back and forth without sticking to each other. At present, a domestic pathology laboratory mostly adopts a method of dripping neutral gum and then sealing with a glass cover plate, and naturally drying at normal temperature, and the sliced slices can be completely dried after being horizontally laid for 2-3 months, so that the method is time-consuming and occupies a lot of space. Before it is completely dried out, the pathological section is not easy to use for full-film digital scanning (easy to slide or card), is not easy to use for patient to take consultation by the section to the external unit and to reserve expert, is not easy to store (easy to adhere with other sections), and the neutral gum used in the conventional staining sealing sheet is an adhesive which adheres the glass slide and the cover glass together and seals the biological tissue section for long-term reservation. The sealing method is very simple, 2-3 drops of gum liquid (note that the gum liquid is dropped together or bubbles are easy to exist) are dropped at the center of the glass slide, then the cover glass is slowly put on, the cover glass is gently squeezed, the gum liquid can spread outwards to the whole lens, and after ventilation and airing for a plurality of hours, the extra gum at the edge of the lens is scrubbed cleanly by using cotton balls to stick xylene or turpentine. Neutral gums are ideal binders with the advantages of high transparency, almost as clear as optical glass, no corrosion of the glass, because it is neutral, less prone to mold growth, and possibly because the solvent xylene of the neutral gum has a fungicidal effect. However, neutral gums have the disadvantage that they use xylene as a solvent and evaporate slowly after encapsulation, and the slides produced cure completely without the smell of xylene at least by airing for 2-3 months in a ventilated place;
the hazards of xylenes are well known and are also being appreciated by the physician technicians in the pathology department. The HE slice just dyed and sealed is sent to a doctor's office, a diagnostician can only observe and read the slice under a microscope immediately in order to report the time, hundreds of slices are stacked on the periphery, if the office environment is not well ventilated, nausea and dizziness poisoning easily occur, and many doctor's offices are not required to open an air conditioner and are also required to open windows for ventilation, so that the physical and mental health is seriously affected after long-term work under the toxic environment. A batch of reports are not sent out, a batch of new slices are also needed to be stored in a special slicing airing cabinet for 2-3 months to volatilize and air, so that a great amount of space of a pathology department is occupied on one hand, and toxic and harmful gases are continuously emitted on the other hand. The traditional sealing method has the advantages of low material consumption, and the cost of sealing each slice is about 0.30 yuan (0.25+0.05) by using the glass cover glass and the neutral resin for sealing the slice. The defect is obvious that xylene pollutes the environment, damages health, and the slicing naturally dries and stores the occupied space, thereby influencing the working efficiency.
Thus, a neutral gum free rapid cure coverslip for pathological sections was proposed.
Disclosure of Invention
The invention aims to provide a neutral gum-free rapid curing cover glass for pathological sections, which solves the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions: a neutral gum free rapid curing coverslip for pathological sections, comprising a slide, a coverslip disposed above the slide, a coating coated below the coverslip;
the top surface of the glass slide is provided with a dyed slice, and xylene is dripped outside the dyed slice.
Preferably, the cover glass adopts ultrathin glass with flat surface, uniform thickness, proper toughness and hardness and excellent optical performance.
Preferably, the coating material is uniformly coated on the ultrathin glass from the middle to the edge, and finally, the ultrathin glass is quickly solidified.
Preferably, the coating material adopts a high polymer resin material capable of being rapidly cured, and the sealing sheet does not need to be added with neutral gum and does not contain aromatic solvents.
Preferably, the laser cutting process is adopted to divide the coated ultrathin glass into cover slips with consistent sizes, and the method is suitable for various existing sealing machines and manual sealing modes of pathology departments, unlike the imported adhesive tape sealing method which requires expensive special imported sealing machines and adhesive tape consumables, is suitable for sealing after biological slice dyeing in all hospitals, scientific research institutions, various laboratories and inspection stations, and has huge social and economic benefits.
Preferably, the cover glass is packaged into the neutral gum-free quick-curing cover glass by adopting a vacuum packaging method, and the sealing piece has better transparency and optical performance than an imported sealing piece machine adhesive tape, does not fade or yellow, and does not form a destructive structure on the sealing piece for a long time.
Compared with the prior art, the invention has the beneficial effects that: the neutral gum free rapid curing cover glass for pathological sections,
1) The neutral gum-free rapid curing cover glass for pathological sections does not need to be added with neutral gum and does not contain aromatic solvents;
2) The neutral gum-free quick-curing cover glass sealing piece for pathological sections can be quickly cured and dried after 10 minutes, so that doctors can read the sections and the patients can consultate the sections by means of the sections, the full-digital scanning and the timely archiving and storage of the sections are facilitated, the drying space of the sections in a department is saved, and the harm to staff and surrounding environment caused by long-term volatilization of harmful gases is avoided;
3) Compared with imported sealing machine adhesive tapes, the neutral gum free rapid curing cover glass sealing piece for pathological sections has more excellent transparency and optical performance, does not fade and yellow, and does not form destructive structures on the sections for a long time;
4) The neutral gum free rapid curing cover glass for pathological sections is applicable to various existing sealing machines and manual sealing modes of pathological departments, does not need expensive special import sealing machines and adhesive tape consumables unlike import adhesive tape sealing, is suitable for sealing after biological sections of all hospitals, scientific research institutions, various laboratories and inspection stations are dyed, and has huge social and economic benefits.
Drawings
FIG. 1 is a schematic view of a portion of a neutral gum free rapid curing coverslip of the present invention;
FIG. 2 is a schematic development of the neutral gum free rapid curing coverslip structure of the present invention.
Description of the embodiments
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples
Referring to fig. 1-2, the present invention provides a technical solution: the neutral gum-free rapid curing cover glass for pathological sections comprises a glass slide, wherein a cover glass is arranged above the glass slide, a coating is coated below the cover glass, a coating material is coated on ultrathin glass and uniformly smeared from the middle to the edge, and finally, the ultrathin glass is rapidly cured;
the coating material adopts a high polymer resin material capable of being rapidly cured, the sealing piece does not need to be added with neutral gum, and the sealing piece does not contain aromatic solvents;
the top surface of the glass slide is provided with a dyed slice, and xylene is dripped outside the dyed slice;
the neutral gum-free quick-curing cover glass sealing piece for pathological sections can be quickly cured and dried after 10 minutes, so that a doctor can conveniently read the sections and a patient can consultate the sections by means of external borrowing, the sections are favorable for full-digital scanning and timely filing and storage, the space for airing the sections in a department is saved, the harm to staff and surrounding environment caused by long-term volatilization of harmful gases is avoided, the problem that a diagnostician can only observe the sections under a microscope immediately in order to report the time is effectively solved, hundreds of sections are piled up on the periphery, and the phenomenon of nausea and dizziness easily occurs if the ventilation of the environment in an office is poor;
the cover glass adopts ultrathin glass with flat preferable surface, uniform thickness, proper toughness and hardness and excellent optical performance;
compared with imported sealing machine adhesive tapes, the neutral gum free rapid curing cover glass sealing piece for pathological sections has more excellent transparency and optical performance, does not fade and yellow, and does not form destructive structures on the sections for a long time;
the coated ultrathin glass is divided into cover slips with consistent sizes by adopting a laser cutting process, and the cover slips are packaged into neutral gum free quick curing cover slips by adopting a vacuum packaging method.
When the adhesive is used, the high polymer resin material is creatively coated on the glass cover glass, so that the adhesive can be used for directly sealing the pathological section after dyeing, neutral gum is not required to be added, a special sealing machine and drying equipment are not required, and the section can be quickly cured within 10 minutes after sealing.
The beneficial effects are that: the neutral gum-free rapid curing cover glass for pathological sections does not need to be added with neutral gum and does not contain aromatic solvents; the neutral gum-free quick-curing cover glass sealing piece can be quickly cured and dried after 10 minutes, so that a doctor can read the piece and a patient can check the piece by means of the slicing, the full digital scanning and timely archiving and storage of the piece are facilitated, the space for drying the piece in a department is saved, the harm to workers and surrounding environment caused by long-term volatilization of harmful gas is avoided, the neutral gum-free quick-curing cover glass sealing piece has more excellent transparency and optical performance than an imported sealing piece machine adhesive tape, the color and yellowing are avoided, a destructive structure is not formed on the piece for a long time, the neutral gum-free quick-curing cover glass is applicable to various existing sealing piece machines and manual sealing piece modes in a pathology department, the imported adhesive tape sealing piece is not required to be expensive, and the adhesive tape sealing piece is suitable for sealing pieces after biological slice dyeing in all hospitals, scientific research institutions, various laboratories and inspection places, and has huge social and economic benefits.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A neutral gum free rapid curing coverslip for pathological sections comprising a slide, characterized in that: a cover glass is arranged above the glass slide, and a coating is coated below the cover glass;
the top surface of the glass slide is provided with a dyed slice, and xylene is dripped outside the dyed slice.
2. A neutral gum free rapid curing coverslip for pathological sections according to claim 1, characterized in that: the cover glass adopts ultrathin glass with flat preferable surface, uniform thickness, proper toughness and hardness and excellent optical performance.
3. A neutral gum free rapid curing coverslip for pathological sections according to claim 1, characterized in that: the coating material is uniformly coated on the ultrathin glass from the middle to the edge, and finally, the ultrathin glass is rapidly solidified.
4. A neutral gum free rapid curing coverslip for pathological sections according to claim 1, characterized in that: the coating material adopts a high polymer resin material capable of being rapidly cured.
5. A neutral gum free rapid curing coverslip for pathological sections according to claim 1, characterized in that: the coated ultrathin glass is divided into cover slips with consistent sizes by adopting a laser cutting process.
6. A neutral gum free rapid curing coverslip for pathological sections according to claim 5, characterized in that: the cover glass is packaged into the neutral gum free quick curing cover glass by adopting a vacuum packaging method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311796348.1A CN117705533A (en) | 2023-12-25 | 2023-12-25 | Neutral gum-free rapid curing cover glass for pathological sections |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311796348.1A CN117705533A (en) | 2023-12-25 | 2023-12-25 | Neutral gum-free rapid curing cover glass for pathological sections |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117705533A true CN117705533A (en) | 2024-03-15 |
Family
ID=90149593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311796348.1A Pending CN117705533A (en) | 2023-12-25 | 2023-12-25 | Neutral gum-free rapid curing cover glass for pathological sections |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117705533A (en) |
-
2023
- 2023-12-25 CN CN202311796348.1A patent/CN117705533A/en active Pending
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