CN117695342A - Traditional Chinese medicine composition for resisting anxiety, and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for resisting anxiety, and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to an anxiolytic traditional Chinese medicine composition, and a preparation method and application thereof, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 40-60 parts of radix rehmanniae, 40-60 parts of armillaria mellea and 20-30 parts of spina date seed; the preparation method of the traditional Chinese medicine composition comprises the following steps: grinding Armillariella mellea and semen Ziziphi Spinosae into powder, decocting radix rehmanniae with water, precipitating with ethanol, and mixing the radix rehmanniae extract and powder to obtain suspension, lyophilizing, and pulverizing. The traditional Chinese medicine composition is proved to be capable of remarkably reducing the number of autonomous activities of mice, remarkably increasing the times of entering the open bin and the residence time of the open bin of anxiety mice, remarkably reducing the sleep latency of the mice and remarkably prolonging the sleep time of the mice through a mouse autonomous activity test, a mouse light-dark box test and a mouse sedative sleep test, and is remarkable in anxiolytic effect and capable of being used for preparing anxiolytic drugs or health care products.
Description
Technical Field
The invention belongs to the technical field of biological medicines, and relates to a traditional Chinese medicine composition for resisting anxiety, and a preparation method and application thereof.
Background
Anxiety is a normal emotional response in humans, and refers to a normal range of psychological emotions with continual feelings of tension, anxiety and fear, whereas anxiety is a disorder, namely anxiety neurosis, which is a neurological disorder characterized by widespread and persistent anxiety or recurrent panic. According to World Health Organization (WHO) statistics, a global prevalence of anxiety disorders was about 3.6% by a total of 2.64 million people worldwide in 2015 (Garakani et al 2020).
The incidence of anxiety disorder also varies greatly with sex. Global female prevalence is 4.6%, far above 2.6% of global male prevalence, whereas in america this difference in male and female prevalence is more pronounced, 7.7% and 3.6% respectively (James et al 2018). Age is also an important factor affecting the incidence of anxiety, which varies from stage to stage. In China, site phobia can occur in childhood, the incidence peak is mostly teenagers and early adulthood, the average incidence age is 17 years, and 2/3 patients develop before 35 years (health committee 2020).
The Chinese medical science has no "anxiety disorder" and the disease is classified as heart disease. In the ancient books of traditional Chinese medicine, there are many records about the disease, for example, in the book of "internal menstruation," the mind is hurt by the ease of mind, the fear is self-lost by the fear, and in the book of "the life is extremely worried, the blood is dissipated by the self-loss, and the mind is unconscious. "mental confusion" and "fear self-loss" are high summaries of anxiety, fear's psychological state, and its dull mental activities. According to the clinical symptoms, the disease is judged to be "mental disease" and "heart disease". The traditional Chinese medicine has the characteristics of less adverse reaction, high safety and the like when being used for treating anxiety, and more traditional Chinese medicines with the anti-anxiety effect are discovered at present, and the traditional Chinese medicines comprise shizandra berry, cortex albiziae, rehmannia root, uncaria, gastrodia elata and the like.
Disclosure of Invention
The primary aim of the invention is to provide a traditional Chinese medicine composition for resisting anxiety, and a preparation method and application thereof, so as to provide a composition which has obvious anxiolytic effect, simple and convenient composition, convenient administration, high safety and less adverse reaction and is easy to accept by patients.
In order to achieve the above purpose, in the basic technical scheme, the invention provides a traditional Chinese medicine composition for resisting anxiety, which comprises the following raw materials in parts by weight:
40-60 parts of radix rehmanniae,
40-60 parts of armillaria mellea,
20-30 parts of wild jujube seed.
Further, in the raw materials of the traditional Chinese medicine composition, the weight ratio of radix rehmanniae, armillaria mellea and spina date seed is 2:2:1.
Further, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 50 parts of radix rehmanniae, 50 parts of armillaria mellea and 25 parts of spina date seed.
The second object of the present invention is to provide a method for preparing the anxiolytic traditional Chinese medicine composition, which comprises the following steps:
s1, weighing raw materials: weighing radix rehmanniae, armillaria mellea and semen Ziziphi Spinosae according to the specified weight parts;
s2, preparing medicinal powder of armillaria mellea and spina date seed: grinding semen Ziziphi Spinosae and Armillariella Mellea, and pulverizing to obtain powder of Armillariella Mellea and semen Ziziphi Spinosae;
s3, preparing a radix rehmanniae extract: extracting radix rehmanniae by water extraction and alcohol precipitation decoction process to obtain radix rehmanniae extract;
s4, mixing the armillaria mellea, the medicinal powder of the wild jujube and the radix rehmanniae extract, transferring the formed suspension to a freeze dryer for drying and crushing to obtain the traditional Chinese medicine composition.
In step S2, the powder of the honey fungus and the jujube kernel is obtained by grinding the jujube kernel and the honey fungus, pulverizing, and sieving with a 100-mesh sieve.
Further, in step S3, the method for preparing the radix rehmanniae extract by adopting the water extraction and alcohol precipitation water decoction process comprises the following specific steps:
s31, water extraction: adding 6-8L of water into every 100g of radix rehmanniae, soaking radix rehmanniae in water for 10-14 hours, extracting at 100deg.C for 1.5-2.5 hours, cooling, collecting supernatant, and concentrating under reduced pressure to obtain water concentrate;
s32, alcohol precipitation: adding ethanol for medicine into the water extraction concentrated solution to obtain ethanol precipitation mixed solution, standing, and taking ethanol precipitation supernatant;
s33, decocting in water: and then decocting the supernatant fluid of the alcohol precipitation with water, and concentrating until the relative density is 1.05-1.08, thus obtaining the radix rehmanniae extract.
A third object of the present invention is to provide the use of the said Chinese medicinal composition for anxiolytic in the preparation of anxiolytic products.
Further, the anxiolytic product is an anxiolytic or a health product.
Further, the anxiolytic product is an anxiolytic honeyed pill prepared by adding auxiliary materials into the anxiolytic traditional Chinese medicine composition.
Further, the auxiliary materials comprise starch and honey.
The anti-anxiety traditional Chinese medicine composition provided by the invention has the beneficial effects that the anti-anxiety traditional Chinese medicine composition, as well as the preparation method and the application thereof, comprises the following raw materials in parts by weight: 40-60 parts of radix rehmanniae, 40-60 parts of armillaria mellea and 20-30 parts of spina date seed; the preparation method of the traditional Chinese medicine composition comprises the following steps: grinding Armillariella mellea and semen Ziziphi Spinosae into powder, decocting radix rehmanniae with water, precipitating with ethanol, and mixing the radix rehmanniae extract and powder to obtain suspension, lyophilizing, and pulverizing. The traditional Chinese medicine composition is proved to be capable of remarkably reducing the number of autonomous activities of mice, remarkably increasing the times of entering the open bin and the residence time of the open bin of anxiety mice, remarkably reducing the sleep latency of the mice and remarkably prolonging the sleep time of the mice through a mouse autonomous activity test, a mouse light-dark box test and a mouse sedative sleep test, and is remarkable in anxiolytic effect and capable of being used for preparing anxiolytic drugs or health care products.
Drawings
Fig. 1 is a schematic diagram of a preparation method of a traditional Chinese medicine composition for resisting anxiety according to an embodiment of the invention.
Detailed Description
The technical solutions of the embodiments of the present invention will be further clearly and completely described below with reference to the accompanying drawings and examples, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and all other examples obtained by those skilled in the art without making creative efforts based on the examples in the present invention are all within the scope of protection of the present invention.
The raw materials of the traditional Chinese medicine composition for resisting anxiety provided by the embodiment mainly comprise radix rehmanniae, armillaria mellea and spina date seed. In the technical conception of the application, the raw materials used in the traditional Chinese medicine composition are screened from anxiolytic prescriptions, and the efficacy and the application of the raw materials comprise: the radix rehmanniae can nourish yin, clear heat, cool blood and enrich blood, and is mainly used for treating febrile polydipsia, internal heat diabetes, bone steaming and fatigue heat, warm disease and spots, blood deficiency and fever, frequent hunger and discouragement, listlessness and lying, chest and diaphragm stuffiness and oppression, hematemesis caused by blood heat and the like; armillaria mellea has effects in calming endogenous wind, suppressing hyperactive liver, dispelling pathogenic wind, dredging collaterals, strengthening tendons and bones, and can be used for treating dizziness, headache, insomnia, numbness of limbs, lumbago and skelalgia, coronary heart disease, hypertension, vascular headache, vertigo syndrome, and epilepsy; semen Ziziphi Spinosae has effects in suppressing hyperactive liver, regulating qi-flowing, moistening lung, nourishing yin, warming spleen and stomach, promoting diuresis, astringing qi, relieving sweating, astringing qi, tranquilizing mind, nourishing marrow, regulating stomach, and activating spleen, and can be used for treating vexation, insomnia, palpitation, sweating, and polydipsia.
The traditional Chinese medicine composition provided by the embodiment is not based on simple superposition of single traditional Chinese medicine effects, but the three traditional Chinese medicines of radix rehmanniae, armillaria mellea and spina date seed supplement each other, and the traditional Chinese medicine composition has the effects of calming and resisting anxiety and is mainly used for treating anxiety, vexation, insomnia and the like.
Unless otherwise indicated, all materials or reagents used in the present embodiment are commercially available products or preparation methods well known to those skilled in the art.
The embodiment provides a traditional Chinese medicine composition for resisting anxiety, which comprises the following raw materials in parts by weight:
40-60 parts of radix rehmanniae,
40-60 parts of armillaria mellea,
20-30 parts of wild jujube seed.
In a specific embodiment, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 50 parts of radix rehmanniae, 50 parts of armillaria mellea and 25 parts of spina date seed.
As shown in fig. 1, the present embodiment also provides a preparation method of a traditional Chinese medicine composition for anxiolytic, the method comprising the following steps:
s1, weighing raw materials: weighing radix rehmanniae, armillaria mellea and semen Ziziphi Spinosae according to the specified weight parts;
s2, preparing medicinal powder of armillaria mellea and spina date seed: grinding semen Ziziphi Spinosae and Armillariella Mellea, and pulverizing to obtain powder of Armillariella Mellea and semen Ziziphi Spinosae;
in a specific embodiment, the powder of the honey fungus and the jujube kernel is obtained by grinding the jujube kernel and the honey fungus, pulverizing, and sieving with a 100-mesh sieve.
S3, preparing a radix rehmanniae extract: extracting radix rehmanniae by water extraction and alcohol precipitation decoction process to obtain radix rehmanniae extract;
in a specific embodiment, the method for preparing the radix rehmanniae extract by adopting the water extraction and alcohol precipitation water decoction process comprises the following specific steps:
s31, water extraction: adding 6-8L of water into every 100g of radix rehmanniae, soaking radix rehmanniae in water for 10-14 hours, extracting at 100deg.C for 1.5-2.5 hours, cooling, collecting supernatant, concentrating under reduced pressure to 1/3 of the volume of the supernatant (within 10% of the deviation), and collecting water concentrate;
s32, alcohol precipitation: adding 95% medicinal ethanol into the water extraction concentrate to obtain ethanol precipitation mixed solution, enabling the final concentration of ethanol in the ethanol precipitation mixed solution to be 80%, standing for 24 hours, and taking ethanol precipitation supernatant;
s33, decocting in water: and then decocting the supernatant fluid of the alcohol precipitation with water, and concentrating until the relative density is 1.05-1.08, thus obtaining the radix rehmanniae extract.
S4, mixing the armillaria mellea, the medicinal powder of the wild jujube and the radix rehmanniae extract, transferring the formed suspension to a freeze dryer for drying and crushing to obtain the traditional Chinese medicine composition.
Example 1 preparation of anxiolytic Chinese medicinal composition
S11, weighing raw materials: weighing 100g of radix rehmanniae, 100g of armillaria mellea and 50g of semen zizyphi spinosae; .
S12, preparing medicinal powder of armillaria mellea and spina date seed;
grinding and pulverizing 50g of spina date seed and 100g of armillaria mellea, and sieving with a 100-mesh sieve to obtain medicinal powder of the armillaria mellea and the spina date seed.
S13, preparing a radix rehmanniae extract;
s131, water extraction: adding 8L of water into 100g of radix rehmanniae, soaking the radix rehmanniae in water for 12 hours, extracting for 2 hours at 100 ℃, cooling, taking water extract supernatant, concentrating the water extract supernatant under reduced pressure to about 1/3 of the volume of the raw water extract supernatant (within 10% of the deviation), and obtaining water extract concentrate;
s132, alcohol precipitation: adding 95% medicinal ethanol into the water extraction concentrate to obtain ethanol precipitation mixed solution, enabling the final concentration of ethanol in the ethanol precipitation mixed solution to be 80%, standing for 24 hours, and taking ethanol precipitation supernatant;
s133, decocting with water: and then decocting the supernatant fluid of the alcohol precipitation with water, and concentrating until the relative density is 1.05-1.08, thus obtaining the radix rehmanniae extract.
S14, mixing the armillaria mellea, the medicinal powder of the wild jujube and the radix rehmanniae extract, transferring the formed suspension to a freeze dryer for drying and crushing to obtain the traditional Chinese medicine composition.
Example 2 mouse voluntary Activity test
2.1 test instruments and reagents
Diazepam, purchased from shandong friendship pharmaceutical limited;
sodium carboxymethyl cellulose, available from the merck group;
the self-made cuboid empty box with length of 100 x and width of 50cm is provided, black paint is fully coated on the inner part of the empty box to form black, and white adhesive tape is used for marking the bottom part of the empty box to form 10 x 10cm small cells.
2.2 method for testing autonomous activity of mice
ICR mice (Beijing vitamin Toril laboratory animal technology Co., ltd., license number: SCXK (Beijing) -2021-0011), 18-22 g weight, 50 male, 5 per cage, raising environment room temperature 20-25 ℃, relative humidity 40-70%, adapted 7d after experiment.
Mice were randomly divided into 5 groups according to body weight, each group being 10, and labeled as control group, positive group, drug high dose group, drug medium dose group, and drug low dose group, respectively. After the experiment is started, the high-dose group, the medium-dose group and the low-dose group are subjected to gastric administration for 7d, wherein the administration doses of the high-dose group, the medium-dose group and the low-dose group are respectively 32g, 16g and 8g crude drugs (the traditional Chinese medicine composition described in the embodiment 1)/kg, the medicines are dissolved by using sodium carboxymethyl cellulose solution (0.5%), and the administration volume is 20ml/kg. The positive and control groups were intragastrically administered the same volume of carboxymethyl cellulose solution. One week later, an voluntary activity test was started, 40min before the test, and the positive group animals were perfused with 4mg/kg of diazepam sodium carboxymethyl cellulose solution.
The number of standing and activity (number of cross) was recorded within 5min for each mouse. After the end of each set of experiments, the next set of experiments was performed after spraying alcohol to remove the odor.
2.3 mice light and dark box test method
Mice in the autonomous activity test described in 2.2 above were used in the light and dark box test. The principle of the light box is based on the psychological conflict of natural aversion emotion of rodents to the open environment and spontaneous exploring behaviors of novel environments, and a box model for determining the anxiolytic activity of the drug by counting the number of times of activities of test animals in the open box and the residence time of the test animals in the open box is taken as evaluation indexes, so that the light box is an important animal model for discovering and screening novel anxiolytic drugs. The whole experiment was carried out in a dark and quiet room and a 40W incandescent lamp and photographic device were mounted above the model light box section. After the last gastric lavage administration for 1h, the mice are sequentially placed in the central area of the open box in a posture that the heads face towards the camera bellows, and the activity condition of the mice in the open box within 5min is counted, wherein the activity condition comprises total residence time and entrance times.
2.4 mice sleep experiment method
ICR mice (Beijing vitamin Toril laboratory animal technology Co., ltd., license number: SCXK (Beijing) -2021-0011), 18-22 g weight, 100 male, 5 per cage, raising environment room temperature 20-25 ℃, relative humidity 40-70%, adapted 7d after experiment.
Mice were randomly divided into 5 groups, each of 20, labeled as control, positive, high, medium, low, respectively, according to body weight. After the experiment is started, the high-dose group, the medium-dose group and the low-dose group are subjected to gastric administration for 7d, wherein the administration doses of the high-dose group, the medium-dose group and the low-dose group are respectively 32g, 16g and 8g crude drugs (namely the traditional Chinese medicine composition described in the embodiment 1)/kg, the drugs are dissolved by using sodium carboxymethyl cellulose solution (0.5%), and the administration volume is 20ml/kg. The positive and control groups were intragastrically administered the same volume of carboxymethyl cellulose solution. After one week, starting a sleep experiment of the mice, and 40 minutes before the experiment, and perfusing the positive group animals with 4mg/kg of diazepam sodium carboxymethyl cellulose solution; half of animals were intraperitoneally injected with a 37mg/kg dose of sodium pentobarbital physiological saline solution, while recording the time from 1min to 30min after disappearance of the eversion and specular reflection; the other half of the animals were intraperitoneally injected with a 50mg/kg dose of sodium pentobarbital physiological saline.
The sleep time and wake-up time (sleep time is based on disappearance of the anti-specular reflection) of each animal, and wake-up time is based on eyelid activity) were recorded.
2.5 test results
The self-activity test carried out in the self-activity box can be used for exploring curiosity and exploring performance of mice, normal mice have a certain curiosity on novelty and exploring behaviors, and the exploring behavior activity of anxiety animals in the self-activity box is far more than that of normal animals, so that the test can be used for anxiety behavior research. The results of the independent activity tests (standing times, number of passing) of the mice in the control group, the positive group, the high-dose group, the medium-dose group, and the low-dose group are shown in Table 1, respectively. The test result shows that the standing times and the grid penetrating number of the positive group are obviously reduced, and each dosage group (a high dosage group, a medium dosage group and a low dosage group) of the traditional Chinese medicine composition can obviously shorten the standing times and the grid penetrating number of the anxiety mice, wherein the high dosage group is most obvious and shows a certain dosage effect relationship.
TABLE 1 results of mice spontaneous Activity test
Rodents are naturally happy and dark, fear of open environments, but curiosity of novel environments, and the method takes the number of times of entering an open box for activity and the stay time of the open box as anxiety condition evaluation indexes through statistics of experimental animals. The results of the light and dark box tests (number of times of entering the open box, open box residence time) of the mice in the control group, the positive group, the high-dose group, the medium-dose group, and the low-dose group are shown in Table 2, respectively. The test result shows that the number of times of entering the open bin and the residence time of the open bin of the positive group are obviously increased, and each dosage group of the traditional Chinese medicine composition can obviously increase the number of times of entering the open bin and the residence time of the open bin of the anxiety mice, wherein the effect of the high dosage group of the medicine is most obvious, and a certain dosage effect relationship is shown among the groups.
TABLE 2 test results of the mouse light and dark box
The anxiety animals have long sleep latency (i.e. difficult sleep), and short sleep time, and the embodiment can judge the effect of the drug on promoting and prolonging sleep through sleep test, i.e. can judge the sedative and anxiolytic effects of the drug. The results of the mice sleep tests (fall asleep time, sleep time) in the control group, the positive group, the high-dose group, the medium-dose group, and the low-dose group are shown in table 3, respectively. The test result shows that the sleep latency of the positive group is obviously shortened, the sleep time is obviously reduced, each dosage group of the traditional Chinese medicine composition can obviously increase the sleep time of anxiety mice, and the sleep time (sleep latency) is obviously shortened, wherein the effect of the high-dosage group of the medicine is most obvious, and a certain dosage effect relationship is shown among the groups.
TABLE 3 mouse sleep test results
Based on the above, the embodiment also provides an application of the traditional Chinese medicine composition for resisting anxiety in preparing an anxiolytic product.
Optionally, the anxiolytic product is an anxiolytic or a health product.
Optionally, the anxiolytic product is an anxiolytic honeyed pill prepared by adding auxiliary materials into the anxiolytic traditional Chinese medicine composition.
In a specific embodiment, the auxiliary materials comprise starch and honey.
EXAMPLE 3 preparation of anxiolytic honeyed pills
Adding appropriate amount of starch and Mel into the Chinese medicinal composition prepared in example 1, mixing, wetting with appropriate amount of ethanol, making soft mass, making into honeyed pill, and packaging to obtain honeyed pill.
The above-described embodiments are merely illustrative of the present invention, which may be embodied in other specific forms or with other specific forms without departing from the spirit or essential characteristics thereof. The described embodiments are, therefore, to be considered in all respects as illustrative and not restrictive. The scope of the invention should be indicated by the appended claims, and any changes that are equivalent to the intent and scope of the claims are intended to be encompassed within the scope of the invention.
Claims (10)
1. The traditional Chinese medicine composition for resisting anxiety is characterized by comprising the following raw materials in parts by weight:
40-60 parts of radix rehmanniae,
40-60 parts of armillaria mellea,
20-30 parts of wild jujube seed.
2. The traditional Chinese medicine composition for resisting anxiety according to claim 1, wherein the weight ratio of radix rehmanniae, armillaria mellea and spina date seed in the raw materials of the traditional Chinese medicine composition is 2:2:1.
3. The traditional Chinese medicine composition for resisting anxiety according to claim 1, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 50 parts of radix rehmanniae, 50 parts of armillaria mellea and 25 parts of spina date seed.
4. A method of preparing a chinese medicinal composition for anxiolytic according to any one of claims 1 to 3, characterized in that the preparation method comprises the steps of:
s1, weighing raw materials: weighing radix rehmanniae, armillaria mellea and semen Ziziphi Spinosae according to the specified weight parts;
s2, preparing medicinal powder of armillaria mellea and spina date seed: grinding semen Ziziphi Spinosae and Armillariella Mellea, and pulverizing to obtain powder of Armillariella Mellea and semen Ziziphi Spinosae;
s3, preparing a radix rehmanniae extract: extracting radix rehmanniae by water extraction and alcohol precipitation decoction process to obtain radix rehmanniae extract;
s4, mixing the armillaria mellea, the medicinal powder of the wild jujube and the radix rehmanniae extract, transferring the formed suspension to a freeze dryer for drying and crushing to obtain the traditional Chinese medicine composition.
5. The method for preparing an anxiolytic Chinese medicinal composition according to claim 4, wherein in step S2, the powder of the honey fungus and the spina date seed is obtained by grinding and powdering the spina date seed and the honey fungus and sieving the ground honey fungus and the honey fungus with a 100-mesh sieve.
6. The method for preparing the anti-anxiety traditional Chinese medicine composition according to claim 4, wherein in the step S3, the method for preparing the radix rehmanniae extract by adopting the water extraction and alcohol precipitation water decoction process comprises the following specific steps:
s31, water extraction: adding 6-8L of water into every 100g of radix rehmanniae, soaking radix rehmanniae in water for 10-14 hours, extracting at 100deg.C for 1.5-2.5 hours, cooling, collecting supernatant, and concentrating under reduced pressure to obtain water concentrate;
s32, alcohol precipitation: adding ethanol for medicine into the water extraction concentrated solution to obtain ethanol precipitation mixed solution, standing, and taking ethanol precipitation supernatant;
s33, decocting in water: and then decocting the supernatant fluid of the alcohol precipitation with water, and concentrating until the relative density is 1.05-1.08, thus obtaining the radix rehmanniae extract.
7. Use of a Chinese medicinal composition for anxiolytic according to any one of claims 1-3 for the preparation of an anxiolytic product.
8. Use of a Chinese medicinal composition for anxiolytic according to claim 7 for the preparation of an anxiolytic product, wherein the anxiolytic product is an anxiolytic or a health product.
9. Use of the anxiolytic Chinese medicinal composition according to claim 7 for preparing an anxiolytic product, wherein the anxiolytic product is an anxiolytic honeyed pill prepared by adding an auxiliary material to the anxiolytic Chinese medicinal composition.
10. Use of a Chinese medicinal composition for anxiolytic according to claim 9 for the preparation of an anxiolytic product, wherein the adjuvant comprises starch, honey.
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