CN117683626B - Multi-bacteriocin culture medium temperature-control bubble-reduction storage protection tank - Google Patents
Multi-bacteriocin culture medium temperature-control bubble-reduction storage protection tank Download PDFInfo
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- CN117683626B CN117683626B CN202410141704.4A CN202410141704A CN117683626B CN 117683626 B CN117683626 B CN 117683626B CN 202410141704 A CN202410141704 A CN 202410141704A CN 117683626 B CN117683626 B CN 117683626B
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- 238000003860 storage Methods 0.000 title claims abstract description 61
- 239000001963 growth medium Substances 0.000 title claims abstract description 31
- 230000007246 mechanism Effects 0.000 claims abstract description 83
- 238000000855 fermentation Methods 0.000 claims abstract description 79
- 230000004151 fermentation Effects 0.000 claims abstract description 79
- 229930185156 spinosyn Natural products 0.000 claims abstract description 47
- 230000001105 regulatory effect Effects 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims description 28
- 239000006260 foam Substances 0.000 claims description 27
- 230000001276 controlling effect Effects 0.000 claims description 21
- 239000003638 chemical reducing agent Substances 0.000 claims description 15
- 238000003825 pressing Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 230000001502 supplementing effect Effects 0.000 claims description 5
- 241000868102 Saccharopolyspora spinosa Species 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims 1
- 238000009434 installation Methods 0.000 claims 1
- JFLRKDZMHNBDQS-UCQUSYKYSA-N CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C(=C[C@H]3[C@@H]2CC(=O)O1)C)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C.CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C=C[C@H]3C2CC(=O)O1)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C Chemical compound CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C(=C[C@H]3[C@@H]2CC(=O)O1)C)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C.CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C=C[C@H]3C2CC(=O)O1)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C JFLRKDZMHNBDQS-UCQUSYKYSA-N 0.000 abstract description 25
- 239000005930 Spinosad Substances 0.000 abstract description 25
- 229940014213 spinosad Drugs 0.000 abstract description 25
- 230000000694 effects Effects 0.000 abstract description 9
- 102000004190 Enzymes Human genes 0.000 abstract description 7
- 108090000790 Enzymes Proteins 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000001133 acceleration Effects 0.000 abstract description 2
- 230000005764 inhibitory process Effects 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 6
- 239000012530 fluid Substances 0.000 description 5
- 241001052560 Thallis Species 0.000 description 3
- 230000005059 dormancy Effects 0.000 description 3
- 230000009036 growth inhibition Effects 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- SRJQTHAZUNRMPR-SFGMUSMWSA-N spinosyn-a Chemical compound C([C@@H](OC(=O)C[C@H]1[C@@H]2C=C[C@@H]3C[C@H](C[C@H]3[C@@H]2C=C1C(=O)[C@@H]1C)O[C@H]2[C@@H]([C@H](OC)[C@@H](OC)[C@H](C)O2)OC)CC)CCC1O[C@H]1CC[C@H](N(C)C)[C@@H](C)O1 SRJQTHAZUNRMPR-SFGMUSMWSA-N 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12M45/00—Means for pre-treatment of biological substances
- C12M45/22—Means for packing or storing viable microorganisms
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Abstract
The invention relates to the technical field of spinosad strain storage and fermentation, in particular to a spinosad culture medium temperature control and bubble reduction storage protection tank, which comprises a storage rack, a fermentation mechanism and a storage protection mechanism, wherein the fermentation mechanism for spinosad strain culture and fermentation and the storage protection mechanism for storage protection of spinosad strain are arranged on the storage rack; the fermentation culture of the spinosyns is carried out through the fermentation mechanism, the storage of the spinosyns is carried out through the storage protection mechanism, the fermentation and preservation factors of the spinosyns are regulated according to actual production requirements, the enzyme activities of the spinosyns in the fermentation mechanism and the storage protection mechanism are influenced, the spinosyns in the fermentation mechanism are controlled to enter a normal growth stage and a growth acceleration stage, the spinosyns in the storage protection mechanism are controlled to enter an inhibition growth stage, the storage and protection of the spinosyns after fermentation are realized, and the yield is controlled.
Description
Technical Field
The invention relates to the technical field of spinosad strain storage fermentation, in particular to a spinosad culture medium temperature-control foam-reducing storage protection tank.
Background
Spinosad is an intracellular secondary metabolite of aerobic fermentation of spinosyns, a finished product is white or light off-white solid crystal, and is a mixture of spinosad A component and spinosad D component, and spinosad is a novel broad-spectrum biological pesticide with contact killing and ingestion toxicity, and is a microbial source biochemical pesticide.
When the spinosad strain is fermented and cultivated, the fermentation period of the spinosad strain is fixed, and when the spinosad strain is fermented, the spinosad is required to be extracted within a specific time, but in industrial production, the fermentation period of the spinosad strain cannot be freely controlled and stored for a long time according to different orders and production requirements;
During fermentation, some gases, such as carbon dioxide and hydrogen, are generated when the spinosad strains metabolize organic substances, and the gases are trapped in the fermentation product to form bubbles, and some substances generated by spinosad metabolism, such as protein and polysaccharide, have surface activity, can reduce the tension of the surface of liquid, so that the bubbles are easier to form and stabilize, air can be stirred in during stirring operation, so that bubbles are formed, the formation of bubbles can be influenced by the nature of the fermentation product, excessive bubbles can occupy the space of the fermentation product, the growth and metabolism of the spinosad strains are influenced, the fermentation effect is influenced, excessive bubbles can cause overflow and pollution, meanwhile, the existing defoaming structure is fixed in the middle of the first tank, and the bubbles in the first tank are required to be accumulated to a certain extent for defoaming.
Disclosure of Invention
The invention solves the technical problems by providing a temperature-control bubble-reducing storage protection tank for a spinosad culture medium.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the temperature-control bubble-reducing storage protection tank for the spinosad culture medium comprises a storage rack, a fermentation mechanism and a storage protection mechanism, wherein the fermentation mechanism for the culture and fermentation of spinosad strains and the storage protection mechanism for the storage protection of spinosad strains are arranged on the storage rack;
The fermentation mechanism comprises a first tank body and a tank cover, a first discharge valve is arranged at the bottom of the first tank body, a support is arranged at the top of the tank cover, a speed reducer is arranged on the support, the output end of the speed reducer is positioned in the fermentation mechanism and is provided with a stirring shaft, stirring blades are arranged on the stirring shaft, the input end of the speed reducer is connected with the output end of a first motor, and a foam reducing component for reducing foam and defoaming is arranged in the fermentation mechanism;
The storage protection mechanism comprises a plurality of second tank bodies, the top of each second tank body is communicated with the first discharge valve through a control valve and a feed pipe, and the bottom of each second tank body is provided with a second discharge valve;
the utility model discloses a jar of high-pressure gas turbine, including first jar of body, a plurality of second jar of body, first jar of body and a plurality of second jar of body are internal all installs spiral coil pipe, and spiral coil pipe both ends communicate with the nozzle respectively, thermometer and manhole are all installed to the internal all of first jar of body and a plurality of second jar, install manometer and a plurality of charging mouth on jar lid and the a plurality of second jar of body.
Preferably, the feeding nozzles are respectively used for adding culture medium and saccharopolyspora spinosa in the fermentation mechanism and the storage protection mechanism, adjusting tank pressure, adjusting air flow, controlling dissolved oxygen, supplementing water and adjusting PH, the speed reducer and the first motor are used for controlling stirring rotation speed, and the spiral coil is used for controlling the internal temperature of the fermentation mechanism.
Preferably, the bubble reducing assembly comprises a rotating plate sleeved on the stirring shaft, a wall body is arranged at the bottom side of one end of the rotating plate, a thorn-shaped sharp corner structure is arranged on the inner wall of the wall body, a scattering device is rotatably arranged in the wall body, and a wall breaking area is arranged between the wall body and the scattering device.
Preferably, the fixed pipe is installed to the wall body bottom side through the support arm, fixed pipe bottom slip sheet is equipped with the movable pipe, the accommodating cover is installed to the movable pipe bottom.
Preferably, the fixed column is installed to the other end downside of revolving plate, the inside slidable mounting traveller of fixed column bottom, traveller bottom elastic mounting has the adjusting column, the clamp plate is installed to adjusting column bottom, and clamp plate outside radian is the same with first jar inner wall radian.
Preferably, the breaker comprises an impeller, a connecting shaft is arranged at the top of the impeller, first rotating teeth are arranged at the top end of the connecting shaft penetrating through the rotating plate, a first inner ring is arranged on the inner wall of the tank cover, and inner teeth meshed with the first rotating teeth are arranged on the inner side of the first inner ring.
Preferably, the sliding column bottom is provided with a movable cavity, a spring is arranged in the movable cavity, and the spring is connected with the adjusting column.
Preferably, a second inner ring is arranged in the first tank body, a guide ring is arranged on the inner side of the second inner ring, and guide sleeves sliding along the guide ring are arranged on the movable pipe and the sliding column.
Preferably, the pinion rack is installed to second inner ring top side symmetry, the different sides of pinion rack are provided with the external tooth respectively, two second motors are installed to first jar external side, the second is changeed the tooth to the second motor output, and the second changes tooth and external tooth meshing.
A temperature-control and bubble-reduction storage protection tank for a spinosyn culture medium comprises the following specific operation steps:
Step one: adding a culture medium and spinosyns into a fermentation mechanism through a feeding nozzle, respectively regulating and controlling tank pressure, air flow, dissolved oxygen, water supplementing and pH (potential of hydrogen) regulation in the fermentation mechanism through the feeding nozzle, working through a first motor, regulating speed by a speed reducer to drive a stirring shaft and stirring blades to rotate so as to control stirring speed, controlling the internal temperature of the fermentation mechanism through circulating cold and hot fluid in a spiral coil pipe, influencing the enzyme activity of the spinosyns of different fermentation mechanisms, controlling the spinosyns in the fermentation mechanism to enter a normal growth stage and accelerate the growth stage, controlling the internal temperature of a storage protection mechanism through circulating cold and hot fluid in the spiral coil pipe, influencing the enzyme activity of the spinosyns of different storage protection mechanisms, respectively controlling the spinosyns in different storage protection mechanisms to enter a growth inhibition or dormancy stage, and storing and protecting the spinosyns;
Step two: when the productivity of spinosad is required to be improved, controlling spinosyns in the fermentation mechanism to enter a growth accelerating stage, and when the productivity of spinosad is required to be reduced, adding spinosyns in the fermentation mechanism after fermentation to a storage protection mechanism to enter a growth inhibiting stage, and storing and protecting the spinosyns after fermentation through the fermentation mechanism;
step three: and in the stirring process, the foam overflowed from the culture medium is defoamed by fermenting the saccharopolyspora spinosa through the foam reducing component, and the gas generated in the culture medium is removed by the foam reducing component in the fermentation process, so that the bubbles in the culture medium are eliminated.
The beneficial effects of the invention are as follows: the fermentation culture of the spinosyns is carried out through the fermentation mechanism, the storage of the spinosyns is carried out through the storage protection mechanism, the fermentation and preservation factors of the spinosyns are regulated according to actual production requirements, the enzyme activities of the spinosyns in the fermentation mechanism and the storage protection mechanism are influenced, the spinosyns in the fermentation mechanism are controlled to enter a normal growth stage and a growth acceleration stage, the spinosyns in the storage protection mechanism are controlled to enter an inhibition growth stage, and the storage and protection of the spinosyns after fermentation are realized.
Realize stirring, the rotation of scattering ware and adjust the position of accomodating cover and clamp plate through first motor work, realize accomodating the high adjustment of cover and clamp plate through second motor work simultaneously, realize absorbing the defoaming to the foam of the internal not co-altitude of first jar and different positions through accomodating the cover, carry out compaction operation to the culture medium through the clamp plate, the gas that fermentation produced in the culture medium and stirring entering is gaseous constantly discharged, avoid reserving a large amount of gas in the culture medium and lead to bubble suddenly increase in a large number in the follow-up fermentation process, realize the bubble defoaming that falls.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the structure of the fermentation mechanism of the present invention;
FIG. 3 is a cross-sectional view of the fermentation mechanism of the present invention;
FIG. 4 is a schematic view of a first configuration of a bubble reducing assembly according to the present invention;
FIG. 5 is a schematic view of a second configuration of the bubble reducing assembly of the present invention;
FIG. 6 is an enlarged view of a portion of the area A of FIG. 3 in accordance with the present invention;
fig. 7 is a partial enlarged view of the area B of fig. 3 in accordance with the present invention.
Legend description:
1. a storage rack; 2. a fermentation mechanism; 3. a first tank; 4. a can lid; 5. a spiral coil; 6. a nozzle; 7. a first discharge valve; 8. a thermometer; 9. a charging nozzle; 10. a pressure gauge; 11. a bracket; 12. a speed reducer; 13. a first motor; 14. a stirring shaft; 15. stirring the leaves; 16. a bubble reduction assembly; 17. a rotating plate; 18. a wall body; 19. a scattering device; 20. breaking the wall area; 21. a support arm; 22. a fixed tube; 23. a movable tube; 24. a housing cover; 25. fixing the column; 26. a spool; 27. an adjusting column; 28. a pressing plate; 29. a first rotating tooth; 30. a connecting shaft; 31. a first inner ring; 32. internal teeth; 33. a movable cavity; 34. a spring; 35. a second inner ring; 36. a guide ring; 37. guide sleeve; 38. a toothed plate; 39. external teeth; 40. a second motor; 41. a second rotating tooth; 42. a storage protection mechanism; 43. a second tank; 44. a control valve; 45. a feed pipe; 46. and a second discharge valve.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Specific examples are given below.
Referring to fig. 1-7, a spinosyn culture medium temperature control and bubble reduction storage protection tank comprises a storage rack 1, a fermentation mechanism 2 and a storage protection mechanism 42, wherein the fermentation mechanism 2 for spinosyn strain culture fermentation and the storage protection mechanism 42 for storage protection of spinosyn strain are arranged on the storage rack 1;
The fermentation mechanism 2 comprises a first tank body 3 and a tank cover 4, a first discharge valve 7 is arranged at the bottom of the first tank body 3, a bracket 11 is arranged at the top of the tank cover 4, a speed reducer 12 is arranged on the bracket 11, the output end of the speed reducer 12 is positioned in the fermentation mechanism 2, a stirring shaft 14 is arranged in the fermentation mechanism 2, stirring blades 15 are arranged on the stirring shaft 14, the input end of the speed reducer 12 is connected with the output end of a first motor 13, and a foam reducing assembly 16 for reducing foam and defoaming is arranged in the fermentation mechanism 2;
The storage protection mechanism 42 comprises a plurality of second tank bodies 43, the top of each second tank body 43 is communicated with the first discharge valve 7 through a control valve 44 and a feed pipe 45, a second discharge valve 46 is arranged at the bottom of each second tank body 43, fermented spinosyns enter the second tank bodies 43 through the first discharge valve 7, the control valve 44 and the feed pipe 45 to be stored and foamed, at the moment, the temperature inside the storage protection mechanism 42 is controlled by circulating cold and hot fluid in the spiral coil 5, the enzyme activity of the spinosyns of different storage protection mechanisms 42 is influenced, the spinosyns in different storage protection mechanisms 42 are respectively controlled to enter a growth inhibition or dormancy stage, and the spinosyns are stored and protected;
The first tank body 3 and the second tank bodies 43 are internally provided with the spiral coil 5, two ends of the spiral coil 5 are respectively communicated with the nozzle 6, the first tank body 3 and the second tank bodies 43 are internally provided with the thermometer 8 and the manhole, the tank cover 4 and the second tank bodies 43 are internally provided with the pressure gauge 10 and the charging nozzles 9, the charging nozzles 9 are respectively used for adding culture medium and spinosyns in the fermentation mechanism 2 and the storage protection mechanism 42, adjusting tank pressure, adjusting air flow, controlling dissolved oxygen, supplementing water and adjusting PH, and the speed reducer 12 and the first motor 13 are used for controlling stirring rotation speed, and the spiral coil 5 is used for controlling the internal temperature of the fermentation mechanism 2.
The fermentation mechanism 2 internally mounted is used for reducing the bubble and defoaming falls bubble subassembly 16, fall bubble subassembly 16 includes the revolving plate 17 of suit on (mixing) shaft 14, the wall 18 is installed to revolving plate 17 one end downside, and wall 18 inner wall is provided with thorn form closed angle structure, install the breaker 19 in the wall 18 internal rotation, set up to broken wall district 20 between wall 18 and the breaker 19, wall 18 downside passes through support arm 21 and installs fixed pipe 22, fixed pipe 22 bottom slip cover is equipped with movable pipe 23, take in the cover 24 is installed to movable pipe 23 bottom, above-mentioned breaker 19 includes the impeller, the connecting axle 30 is installed at the impeller top, the connecting axle 30 top runs through revolving plate 17 and installs first rotating tooth 29, first inner ring 31 is installed to tank cover 4 inner wall, first inner ring 31 inboard is provided with the internal tooth 32 with first rotating tooth 29 meshing, when revolving plate 17 rotates, first rotating tooth 29 and first inner tooth 32 cooperation with first inner ring 31 drives connecting axle 30 and take in the position adjustment of cover 24 and clamp plate 28, foam breaker 19 through the fixed sharp angle structure of taking in the breaker 24, the foam is passed through to the breaker 19 on the fixed pipe 23, the breaker is passed through to the breaker 19 to the broken wall district through to the fixed pipe 19, the efficiency is improved in the breaker 19, the foam is passed through to the breaker is taken in the breaker 19 to the breaker is passed through to the breaker 19.
The fixed column 25 is arranged at the bottom side of the other end of the rotating plate 17, the sliding column 26 is arranged in the bottom end of the fixed column 25 in a sliding manner, the adjusting column 27 is elastically arranged at the bottom of the sliding column 26, the pressing plate 28 is arranged at the bottom of the adjusting column 27, the radian of the outer side of the pressing plate 28 is the same as that of the inner wall of the first tank body 3, the second inner ring 35 is arranged in the first tank body 3, the guide ring 36 is arranged on the inner side of the second inner ring 35, the guide sleeve 37 sliding along the guide ring 36 is arranged on the movable tube 23 and the sliding column 26, and when the accommodating cover 24 and the pressing plate 28 rotate, the guide sleeve 37 moves along the guide ring 36 at the moment to play a guiding role;
The toothed plate 38 is symmetrically arranged on the top side of the second inner ring 35, external teeth 39 are respectively arranged on different sides of the toothed plate 38, two second motors 40 are arranged on the outer side of the first tank body 3, second rotating teeth 41 are arranged at the output end of each second motor 40, the second rotating teeth 41 are meshed with the external teeth 39, the second motors 40 work to drive the second rotating teeth 41 to rotate and are matched with the meshed external teeth 39 to drive the toothed plate 38 to lift, and then the lifting of the second inner ring 35 is achieved, at the moment, the movable tube 23 slides on the outer side of the fixed tube 22, the sliding column 26 slides in the fixed column 25, and the heights of the accommodating cover 24 and the pressing plate 28 are adjusted.
The movable cavity 33 has been seted up to the traveller 26 bottom, movable cavity 33 internally mounted has spring 34, and spring 34 is connected with adjusting column 27, when accomodating cover 24 and clamp plate 28's altitude mixture control back, accomodate cover 24 and clamp plate 28 when moving down, clamp plate 28 contacts with the culture medium this moment, and along with clamp plate 28 keeps moving down until accomodating cover 24 is located culture medium top foam department, adjusting column 27 moves up in movable cavity 33, compress spring 34, spring 34 reaction clamp plate 28, compact the culture medium through clamp plate 28.
A temperature-control and bubble-reduction storage protection tank for a spinosyn culture medium comprises the following specific operation steps:
Step one: adding a culture medium and spinosyns into a fermentation mechanism 2 through a feeding nozzle 9, respectively regulating and controlling tank pressure, air flow, dissolved oxygen, water supplementing and PH (potential of hydrogen) regulation in the fermentation mechanism 2 through the feeding nozzle 9, operating through a first motor 13, regulating speed by a speed reducer 12 to drive a stirring shaft 14 and stirring blades 15 to rotate so as to control stirring rotation speed, controlling the internal temperature of the fermentation mechanism 2 through circulating cold and hot fluid in a spiral coil 5, influencing the enzyme activity of the spinosyns of different fermentation mechanisms 2, controlling the spinosyns in the fermentation mechanism 2 to enter a normal growth stage and accelerate the growth stage, controlling the internal temperature of a storage protection mechanism 42 through circulating cold and hot fluid in the spiral coil 5, influencing the enzyme activity of the spinosyns of different storage protection mechanisms 42, respectively controlling the spinosyns in different storage protection mechanisms 42 to enter a growth inhibition, namely a dormancy stage, and storing and protecting the spinosyns;
When the concentration of the thalli is higher than 40%, adding sterilizing water, and controlling the concentration of the thalli of the fermentation liquid to be 30-40%, and detecting the concentration of the thalli of the fermentation liquid every 6-8 hours;
；
step two: when the productivity of spinosad needs to be improved, controlling spinosyns in the fermentation mechanism 2 to enter a growth accelerating stage, and when the productivity of spinosad needs to be reduced, adding the spinosyns in the fermentation mechanism 2 which is completed by fermentation into the storage protection mechanism 42 to enter a growth inhibiting stage, and storing and protecting the spinosyns which are completed by fermentation through the fermentation mechanism 2;
Step three: and in the stirring process, foam that overflows in the culture medium is subjected to defoaming operation through the foam reducing component 16, and the foam produced in the culture medium is removed through the foam reducing component 16 in the fermentation process, the bubbles in the culture medium are eliminated, stirring shaft 14 rotates and drives rotating plate 17 to rotate, the position of housing cover 24 and pressing plate 28 is adjusted in fermentation mechanism 2, when rotating plate 17 rotates, first rotating teeth 29 are matched with inner teeth 32 of first inner ring 31, connecting shaft 30 and diffuser 19 are driven to rotate, foam is sucked through housing cover 24, the foam enters wall breaking area 20 of diffuser 19 through fixed tube 22 and movable tube 23, the foam is thrown on wall 18, defoaming efficiency is improved through the thorn sharp angle structure, second rotating teeth 41 are driven to rotate through the operation of second motor 40, tooth plate 38 is driven to lift with the engaged outer teeth 39, and further realize the lifting of second inner ring 35, at this moment, movable tube 23 slides outside fixed tube 22, slide column 26 slides inside fixed column 25, the height of housing cover 24 and pressing plate 28 is adjusted, foam is sucked into housing cover 24, high-pressure side of foam is compressed, foam is prevented from being pressed out of the culture medium, and the high-pressure side of foam is prevented from being pressed out of the culture medium, and the foam is greatly absorbed by the high pressure of the culture medium.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (2)
1. The temperature-control and bubble-reduction storage protection tank for the spinosyn culture medium is characterized by comprising a storage rack (1), a fermentation mechanism (2) and a storage protection mechanism (42), wherein the fermentation mechanism (2) for culturing and fermenting spinosyn strains and the storage protection mechanism (42) for storing and protecting spinosyn strains are arranged on the storage rack (1); the fermentation mechanism (2) comprises a first tank body (3) and a tank cover (4), a first discharge valve (7) is arranged at the bottom of the first tank body (3), a support (11) is arranged at the top of the tank cover (4), a speed reducer (12) is arranged on the support (11), the output end of the speed reducer (12) is positioned in the fermentation mechanism (2) and is provided with a stirring shaft (14), stirring blades (15) are arranged on the stirring shaft (14), the input end of the speed reducer (12) is connected with the output end of a first motor (13), and a foam reducing assembly (16) for reducing foam is arranged in the fermentation mechanism (2). The storage protection mechanism (42) comprises a plurality of second tank bodies (43), the tops of the second tank bodies (43) are communicated with the first discharge valve (7) through control valves (44) and feed pipes (45), and second discharge valves (46) are arranged at the bottoms of the second tank bodies (43); the novel plastic tank is characterized in that spiral coils (5) are arranged in the first tank body (3) and the plurality of second tank bodies (43), two ends of each spiral coil (5) are respectively communicated with a nozzle (6), a thermometer (8) and a manhole are arranged in the first tank body (3) and the plurality of second tank bodies (43), and a pressure gauge (10) and a plurality of charging nozzles (9) are arranged on the tank cover (4) and the plurality of second tank bodies (43);
the foam reducing assembly (16) comprises a rotating plate (17) sleeved on a stirring shaft (14), a wall body (18) is arranged at the bottom side of one end of the rotating plate (17), a thorn-shaped sharp corner structure is arranged on the inner wall of the wall body (18), a breaker (19) is rotatably arranged in the wall body (18), a wall breaking area (20) is arranged between the wall body (18) and the breaker (19), a fixed pipe (22) is arranged at the bottom side of the wall body (18) through a supporting arm (21), a movable pipe (23) is slidably sleeved at the bottom end of the fixed pipe (22), a containing cover (24) is arranged at the bottom of the movable pipe (23), a fixed column (25) is arranged at the bottom side of the other end of the rotating plate (17), a sliding installation sliding column (26) is arranged at the bottom of the fixed column (25), a regulating column (27) is elastically arranged at the bottom of the sliding column (26), the outer side of the pressing plate (28) is the same as the inner wall of a first tank (3), the impeller (19) in radian, the impeller (28) comprises a movable pipe (23), an impeller (31) and a rotating ring (30) is arranged in the rotating plate (30), the inner side of the first inner ring (31) is provided with inner teeth (32) meshed with the first rotating teeth (29), a movable cavity (33) is formed in the bottom end of the sliding column (26), a spring (34) is mounted in the movable cavity (33), the spring (34) is connected with the adjusting column (27), the second inner ring (35) is mounted in the first tank (3), a guide ring (36) is arranged on the inner side of the second inner ring (35), guide sleeves (37) sliding along the guide ring (36) are mounted on the movable pipe (23) and the sliding column (26), toothed plates (38) are symmetrically mounted on the top side of the second inner ring (35), outer teeth (39) are respectively arranged on different sides of the toothed plates (38), two second motors (40) are mounted on the outer side of the first tank (3), second rotating teeth (41) are mounted at the output ends of the second motors (40), and the second rotating teeth (41) are meshed with the outer teeth (39).
2. The spinosyn culture medium temperature control and bubble reduction storage protection tank according to claim 1, wherein a plurality of charging nozzles (9) are respectively used for adding culture medium and saccharopolyspora spinosa in a fermentation mechanism (2) and a storage protection mechanism (42), adjusting tank pressure, adjusting air flow, controlling dissolved oxygen, supplementing water and adjusting PH, a speed reducer (12) and a first motor (13) are used for stirring rotation speed control, and a spiral coil (5) is used for controlling the internal temperature of the fermentation mechanism (2).
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